JP2003292427A - Deodorant agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a deodorant agent or an apolipoprotein D-secretion inhibitor useful as a highly safe cosmetic or the like having excellent deodorant activities because of sustainably inhibiting the formation of unpleasant body odor. <P>SOLUTION: The deodorant agent or the apolipoprotein D-secretion inhibitor comprises a plant selected from Phellodendri cortex, Phellodendron amurens Ruprecht, Gallae rhois, Rhus javanica L, seeds of Oryza sativa L., Haematoxylon campechianum L., Arnica montana L., Foeniculi fructus, Isodonis herba, Isodon japonicus Hara, Coptidis rhizome, Asiasari radix, Zanthoxyli fructus, Astragalus sinicus, Hedera helix, Cinnamomi cortex, Melissa officinalis, Aesculus hippocastanum, Uncaria gambir Roxburgh, Upharis rhizome, Centella asiatica and Sanguisorba officinalis L, or an extract thereof. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a deodorant agent for suppressing the generation of unpleasant body odor in humans.

[0002]

2. Description of the Related Art Body odors include sweat odor, breath odor, scalp odor, foot odor and the like throughout the body. Among them, especially with regard to the smell of sweat, it is composed of a mixture of an axillary odor represented by "Wakiga" and an acid odor throughout the body. In particular, armpit odor has been increasingly desired as a representative of the odor that causes an aversion, in recent years. Unlike the Ecklin sweat gland, the apocrine glands, which secrete sweat that causes axillary odor, are often found in the axilla, areola, genital area, etc., but are not localized in those areas and are widely present in the trunk. (Pinkus H; Mehregan AH; Adnexal Nevi and Benign
Adnexoid Tumors, in A Guide to Dermatohistopathlo
gy; 2nd ed, pp528, pp29, by Appleton-Century-Croft
s, New York, 1976), and in recent years, when the tendency toward cleanliness is advancing, it is an issue to continuously remove such axillary odor.

It is known that sweat secreted from the apocrine gland does not give off a strong odor by itself, but it is converted into an odor substance by the skin-resident bacteria existing on the skin surface (Leyden JJ, et al. , J Invest Dermatol, 77: 4
13-416, 1981), recently, one of the odor molecules 3
-Methyl-2-hexenoic acid (3M2H) is also reported to be secreted to the skin surface in a form bound to a carrier protein, Apolipoprotein D (Zeng C, et al, Proc Natl Acad Sci). U SA, 93: 6
626-6630, 1996). In addition, the types of odor molecules also vary according to the type of fatty acid (Zeng XN, et al; Analysis of characteristic odors
from human male axillae., J Chem Ecol, 17: 1469-1
492, 1991) and male hormone derivatives (Labows JN. Et a
l., Steroids, 34: 249-258, 1979, Gower DB, et al.,
J Steroid Biochem Mol Biol, 48: 409-418, 1994)
In addition, it has been reported that there are a wide variety.

[0004] On the other hand, as a main method for preventing body odor, a technique for suppressing microorganisms on the body surface (bactericide,
Antibacterial agents, etc., techniques for suppressing sweat secretion by astringent action (astringents such as metal oxides), techniques for deodorizing unpleasant body odors generated, masking techniques for scents, etc. have been reported.

However, none of these techniques fundamentally eliminates the unpleasant body odor represented by the axillary odor, and the effect is not sufficient. Further, according to the antibacterial technique, it is suggested that the primary barrier function of the skin may be lowered by sterilizing not only the bacteria causing the unpleasant odor but also the bacteria resident in the skin.

An object of the present invention is to provide a deodorant agent having high safety and capable of fundamentally suppressing the generation of unpleasant body odors such as sweat odor, particularly axillary odor.

[0007]

Means for Solving the Problems The present inventors have found that if the synthesis and secretion of apolipoprotein D, which is known as one of the carrier proteins in the apocrine sweat glands, is suppressed, the odor molecules that cause axillary odor are present on the skin surface. As a result of investigating a substance that suppresses the synthesis and secretion of the protein, it was found that a specific plant extract has an apolipoprotein D secretion inhibitory action and is useful as a deodorant agent. .

[0008] That is, the present invention is,
Deodorant agent and apolipo consisting of a plant or its extract selected from rice, logwood, arnica, fennel, trillium, laurel, saishin, salamander, forsythia, eucalyptus, cinnamon, melissa, horse chestnut, acacia yak, kohone, Centella asiatica and waremoko. The present invention provides a protein D secretion inhibitor.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION The plants used as the deodorant agent and the apolipoprotein D secretion inhibitor of the present invention are:
Ohabaku (yellow oak PHELLODENDRI CORTEX; Kihada Phellod
endron amurens Ruprecht or other plants of the same genus), Gobaishi (Gallae Rhois); Nurude Rhus javanica L,
Rhus chinensis Miller or insect ray in young shoots or petioles of the same genus plant), rice (seed of rice Oryza sativa L.), logwood ( Haematoxylon campechia)
num L.), Arnica ( Arnica montana L.),
Fenicul fengar FOENICULI FRUCTUS; fennel Foeniculum vulgare Miller, green pea (ISODONIS HERBA; Hikokoshi Isodon japonicu)
s Hara), Coptis (Coptis Coptidis Rhizoma; Coptis Coptis japonica Mkino or other genus plant), the latest (Hosokarashi ASIASARI RADIX; thin leaf Herba Asari Asiasaru
m sieboldii F. Maekawa or Keirin Saishin Asiasaru
m heterotropoides F. Maekawa var. mandshuricum F.
Maekawa), Sansho ( Zanthoxyli Fructus; Zanthoxylum piperitum De Candolle or other plants of the same genus), Astragalus (Lotus grass, florets, astragalus,
Astragalus sinicus ), Ivy ( Hedera h)
elix ), Keihi (CINNAMOMI CORTEX; Cinnamomum ca
ssia Blume or other genus plant), Melissa (western Yamahakka, perfume mint, Melissa saw Melissa o
fficinalis ), horse chestnut (horse chestnut, horse chestnut, horse chestnut Aesculus hippocastanum ), asen yak (Asenyaku Gambir GAMBIR; Uncaria gambir Roxbur)
gh), Kouhone (Senkotsu river bone NUPHARIS RHIZOMA;
Nuphar japonicum De Cancolle or other plants of the same genus), Centella asiatica , Centella asiatica , Warmoko, Sanguisorba officinalis
L.).

[0010] These plants can be used as they are or whole plants or leaves, roots, rhizomes, fruits, seeds and flowers as they are or after being crushed, but the bark is used for psyllium, insect rays for Goba, and seeds and logwood for rice. Is xylem, Arnica roots and flowers, fennel is fruit, Pleurotus cornucopiae is above-ground, Coptis rhizome, Rhizoma is roots and rhizomes, Pleurotus officinalis, astragalus is above-ground, Astragalus ivy is above-ground, Keihi is bark, Melissa Is above ground, horse chestnut is seed, leaves and bark,
It is preferable to use leaves and young shoots for Acacia catechu, rhizomes for Astragalus japonicum, root parts for Centella asiatica and roots and rhizomes for Valeriania japonica. In addition, rice includes rice bran and fermented rice bran fermented with yeast or the like.

In the present invention, the extract means various solvent extracts obtained by further extracting these plants at room temperature or under heating or by using an extractor such as a Soxhlet extractor, and their dilution. It means a liquid, its concentrated liquid or its dried powder. The extract here may be a mixture obtained from two or more plants.

Solvents used for extraction include water; alcohols such as methanol, ethanol, propanol and butanol; polyhydric alcohols such as propylene glycol and butylene glycol; ketones such as acetone and methyl ethyl ketone; esters such as methyl acetate and ethyl acetate. Chain and cyclic ethers such as tetrahydrofuran and diethyl ether; hydrogen halides such as dichloromethane, chloroform and carbon tetrachloride; hexane, cyclohexane,
Examples thereof include hydrocarbons such as petroleum ether; aromatic hydrocarbons such as benzene and toluene; polyethers such as polyethylene glycol; pyridines and the like, and these can be used alone or as a mixture.

Further, it is also possible to remove the inactive contaminants from the above-mentioned extract by a technique such as liquid distribution, and it is preferable to use such a substance in the present invention. If necessary, these may be used after being subjected to treatments such as deodorization and decolorization by known methods.

The plant or its extract can be used as it is as the deodorant agent and the apolipoprotein D secretion inhibitor of the present invention, but the extract is diluted or concentrated or lyophilized, and then powdered or paste. It can also be prepared into a shape and used. Such a plant or its extract reduces the secretory amount of apolipoprotein D, which is a carrier protein for odorous molecules, and exerts an axillary odor suppressing effect, as shown in Examples below. Therefore, these are useful as deodorant agents capable of fundamentally suppressing the generation of odor-causing substances.

The deodorant agent and the apolipoprotein D secretion inhibitor of the present invention are used as preparations for cosmetics, external medicines or quasi drugs, such as creams, emulsions, lotions, powders, sprays, sticks, sheets or compresses. It can be used as an agent, and it is also possible to use several methods in combination.

Deodorant agent or apolipoprotein D when used as cosmetics, external medicines or quasi drugs
Generally, the content of the secretion inhibitor is preferably 0.1 to 20% by weight, and particularly preferably 0.5 to 10% by weight. The content as a plant extract is generally preferably 0.00001 to 10% by weight in terms of solid content, and particularly preferably 0.0005 to 5% by weight.

In these cosmetics, external medicines or quasi-drugs, various components usually used, for example, oils, surfactants, alcohols, chelating agents and pH adjusters generally used as cosmetic ingredients are used. , Preservatives, thickeners, pigments,
In addition to fragrances, UV absorbers, whitening agents, wrinkle improvers, moisturizers, sebum secretion inhibitors, softeners, keratin protectants, medicinal agents, antioxidants, solvents, etc. Can be converted.

Further, each of the above-mentioned preparations contains fine particles of a natural or synthetic porous metal oxide, an astringent compound containing a metal such as aluminum, zirconium or zinc, a bactericide, an antibacterial agent, an antibiotic. The deodorant effect can be enhanced by appropriately mixing a substance and the like.

The deodorant agent and the apolipoprotein D secretion inhibitor of the present invention can control the generation of body odor by being applied to a place where an unpleasant odor is likely to occur, such as the foot, armpit, head and genital area. The amount of the formulation used in such cases is
Depending on the content of the active ingredient, for example, in the case of a liquid preparation, 1 to ²⁰ mg per cm ^{2 of} skin surface, in the case of a solid preparation,
Similarly, it is preferably 1 to 50 mg.

[0020]

EXAMPLES Production Example 1 bark extract of Preparation bark (Phellodendron Phellodendron amurense Ruprecht cork layer excluding bark of) 40 g in 50v / v% aqueous ethanol solution 400mL was added, was extracted at room temperature for 7 days, the extract was filtered Was obtained (yield: 350 mL, evaporation residue: 0.
87 w / v%).

Production Example 2 The plant extracts shown in Table 1 were prepared according to Production Example 1.

[0022]

[Table 1]

Example 1 Apolipoprotein D secretion inhibitory action (1) Cell culture and sample addition Cell culture was carried out using a breast cancer-derived cell line T-47D (ATCC No. HT).
B-133) and 10% FCS-added DMEM medium (LIFE
TECHNOLOGIES company Cat.11995-065), 37 ℃, 5%
-CO _2, were subcultured in a humidified. Inoculate 6 well plate to 40 × 10 ⁴ cells / well, 10%
The cells were cultured for 2 days in DMEM medium supplemented with FCS (charcoal treatment). After removing the medium, PBS (LIFE TECHNOLOGIES
After washing twice with Cat.14190-144), retinoic acid 10
Add DMEM medium containing ^-6 M and 0.1% of various extracts. Two days later, the medium was exchanged, and two days later, the medium was collected and subjected to Western blotting.

(2) Western blotting pretreatment: The floating cells were removed from the collected medium by centrifugation, 400 μL was concentrated by ultrafiltration with Microcon 10, and then sample buffer for electrophoresis (2 mL10% SDS, 2 mL).
mL 2-mercapoethanol, 2.5mL 1M Tris pH 6.8, 3.5mL 7
0% sucrose) (10 μL) was added, the mixture was heated at 95 ° C. for 5 minutes, and then collected by centrifugation at 2000 rpm for 3 minutes. SDS-PAGE: Tris / Glycine / SDS
Buffer (BIO-RAD, Cat.161-0772), 10% S
DS-polyacrylamide gel (BIO-RAD, Cat.161)
-J331), the pre-treated medium concentrate was added per well to Microcon 10 (MILLIPORE, Cat.424).
07) Apply 1 bottle (equivalent to 400 μL of medium), 1
Electrophoresis was performed at a constant current of 0 to 20 mA. Semi-dry blotting: Blotting buffer is Tris / containing 0.01% SDS and 20% methanol.
Glycine buffer (BIO-RAD, Cat.161-073)
Four). Two pieces of blotting filter paper (BIO-RAD, Cat.170-3932) were immersed in one blotting buffer per gel, and both the gel electrophoresed with the methanolized PVDF membrane and the blotting buffer were immersed. Trans-
Blot SD (Cat.170-3957, manufactured by BIO-RAD) is set from bottom to bottom with two filter papers, a PVDF membrane with the front side facing down, a gel with the front side facing down, and two filter papers in that order, 20V, 60 minutes Energized. Blocking: Block Ace (Snow Brand UK-B25)
The membrane washed with PBS after being energized was dipped in.
(For about 20 hours refrigerated storage) Primary antibody: anti-HUMAN Apolipoprotein D (RDI, Ca
t.RDI-APODabm) was added to Block Ace at a 300-fold dilution, and the mixture was shaken at room temperature for 1 hour. Secondary antibody: Membrane 1 x TBST (DAKO, Cat.
It was rinsed twice with S3306 diluted) and further washed with shaking once for 15 minutes and twice for 5 minutes. Anti-mouse IgG F (ab ')
₂ (Amersham Pharmacia, NA931) was used after being diluted 30000 times with Block Ace, and the membrane was immersed and shaken for 1 hour. Detection: The membrane was rinsed twice with 1 × TBST and further washed with shaking once for 15 minutes and twice for 5 minutes. SuperSig
nal West Femto Maximum Sensitivity Substrate (Pier
It was immersed in ce., Cat.34095) for 5 minutes. Then, it was exposed to an autoradiography film to detect apolipoprotein D. Scan the film (EPSON, G
T-9500WIN) and load it to the computer, and then use Lane & Spot
The band density was quantified with an Analyzer (manufactured by ATTO), and the ratio of the amount of apolipoprotein D to the extract-free sample was calculated. The results are shown in Table 2.

[0025]

[Table 2]

Example 2 Body Odor Suppression Test A placebo sample (Table 3) was applied to one armpit of 20 test subjects having strong body odor and an axillary odor, and an oak extract sample (Table 3) was applied to the other armpit. It was applied twice a day for 4 weeks. The body odor was evaluated by two expert panelists, and evaluated the body odor of the axillary part before and after use on the following five grades. The results are shown in Table 4.

[0027] 1: Slightly smell 2: Smell clearly 3: Slightly strong smell 4: Smell strongly 5: Very strong odor

The value obtained by subtracting the score before use from the score after use indicates a decrease in body odor on the side of the oyster, and it was confirmed that the axillary odor was particularly reduced. When the Wilcoxon signed rank test was performed on the score changes in both axils of each subject, there was a significant difference of less than 1% in the risk rate.

[0029]

[Table 3]

[0030]

[Table 4]

From the above, the effect of suppressing the body odor and especially reducing the axillary odor was recognized in the psyllium extract of the present invention.

[0032]

The deodorant agent and the apolipoprotein D secretion suppression of the present invention can suppress the generation of unpleasant body odor continuously, and therefore, cosmetics, external medicines or pharmaceuticals having an excellent deodorizing effect and high safety. It is useful as an external product.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61K 35/78 A61K 35/78 K MN Q T U A61P 17/00 A61P 17/00 F term (reference) 4C083 AA111 AA112 CC17 EE18 4C088 AB12 AB16 AB26 AB32 AB33 AB38 AB39 AB40 AB51 AB59 AB62 AB73 AC01 AC03 AC04 AC05 AC11 AC13 CA03 MA63 NA14 ZA89

Claims (5)

[Claims] 1. Oataku, Gobaishi, rice, logwood, arnica, fennel, trillium, weeping,
Saishin, salamander, lotus root, Ivy,
Keihi, Melissa, horse chestnut, Acenyak, kohone,
A deodorant agent comprising a plant selected from Centella asiatica and Pleurotus cornucopiae or an extract thereof. 2. An oat, an amberjack, a rice, a logwood, an arnica, a fennel, a green alga, an oren,
Saishin, salamander, lotus root, Ivy,
Keihi, Melissa, horse chestnut, Acenyak, kohone,
An apolipoprotein D secretion inhibitor comprising a plant selected from Centella asiatica and Pleurotus cornucopiae or an extract thereof. 3. The deodorant agent according to claim 1, which suppresses body odor. 4. The deodorant agent according to claim 1, which suppresses the odor of sweat. 5. The deodorant agent according to claim 1, which suppresses the odor of the armpit.