JP2003238394A - Allergen-reducing agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an allergen-reducing agent capable of reducing the allergen such as house dust present in the environment without affecting the human body and without causing problems such as coloring. <P>SOLUTION: The allergen-reducing agent comprises a sesquiterpene alcohol. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an allergen reducing agent for reducing allergens in the environment.

[0002]

2. Description of the Related Art Allergic diseases such as atopic dermatitis, allergic rhinitis and asthma have been increasing in recent years and have become an important social problem. An increase in allergens in the environment is one of the causes of the increase in allergic diseases.In particular, with the improvement of the airtightness of the house, the conditions suitable for the reproduction of mites in the room are set, and the indoor space allergen amount including mite allergen Increase is a problem.

For prevention and treatment of allergic diseases, removal of such allergens is a rational means,
Until now, attempts have been made to prevent contact between people and allergens with air purifiers and highly confidential duvet covers,
The effect was not sufficient. Attempts have also been made to reduce the number of mites with acaricides,
The acaricide itself adversely affects the human body, and even if the acaricide itself is killed, allergenicity remains in the feces and the carcass left behind,
It cannot be said that this is a fundamental solution for reducing the amount of allergen. Attempts have also been made to reduce the number of mites by using repellents, but there is a problem with the persistence of the effect, and the number of mites recovers over time, and even if the mites themselves decrease, the remaining feces and their carcasses will remain. Remains allergenic and cannot be said to be a fundamental solution for reducing the amount of allergen.

Further, attempts have been made to chemically inactivate allergens by natural extracts such as tea extract and tannic acid, but there is a problem of coloring the object due to aging and a problem when using a large amount. There is still a problem in safety, and it is difficult to use it in products.

An object of the present invention is to provide an allergen-reducing agent that has no adverse effect on the human body and has no problems such as coloring.

[0006]

[Means for Solving the Problems] The inventors of the present invention searched for a substance which can stably inactivate allergens in the environment and is highly safe. As a result, sesquiterpene alcohol induces an allergic reaction to allergens. It has been found that it has an effect of reducing the ability and is useful as an allergen reducing agent.

That is, the present invention provides an allergen reducing agent comprising a sesquiterpene alcohol.

[0008]

BEST MODE FOR CARRYING OUT THE INVENTION The sesquiterpene alcohol referred to in the present invention is monovalent, divalent, or trivalent having 15 carbon atoms.
Valued terpene alcohol. Sesquiterpene alcohols are roughly classified into acyclic sesquiterpene alcohols and cyclic sesquiterpene alcohols. Cyclic sesquiterpene alcohols are monocyclic sesquiterpene alcohols and 2
It is classified into cyclic sesquiterpene alcohols and tricyclic sesquiterpene alcohols. As a preferable embodiment of the sesquiterpene alcohol, an acyclic sesquiterpene alcohol, a bicyclic or tricyclic sesquiterpene alcohol is more preferable, and an acyclic sesquiterpene alcohol and a tricyclic sesquiterpene alcohol are particularly preferable.

As a preferred specific embodiment of the sesquiterpene alcohol, farnesol and nerolidol are preferred as the acyclic sesquiterpene alcohol, terpineol is preferred as the monocyclic sesquiterpene alcohol, and widrol and guaiol as the bicyclic sesquiterpene alcohol. , Vetiverol are preferable, and as the tricyclic sesquiterpene alcohol, patchouli alcohol, cedrol, and santalol are preferable. Among them, cedrol, farnesol, santalol, and nerolidol are preferable from the viewpoint of the effect.

The sesquiterpene alcohol in the present invention may be extracted (including steam distillate, pressed product, etc.) from the plant containing it and purified, but a synthetic product may also be used. .

Such sesquiterpene alcohol has the action of diminishing or eliminating the antigenicity of mite allergen, as shown in the Examples below. Therefore, the sesquiterpene alcohol of the present invention diminishes or eliminates the ability to induce allergic reaction to various allergens and is useful as an allergen reducing agent.

[0012] Here, the allergen means a substance that causes an allergic reaction such as asthma, allergic rhinitis, hay fever, and atopic dermatitis upon contact with humans and animals. In the present invention, for example, Plant allergens derived from pollen of plants such as cedar, cypress, ragweed, and duckweed, epidermis and hair of animals such as dogs and cats, parasites, insects such as cockroaches and moths, mites such as leopard mites, mites, and sand mites In addition to animal-derived allergens derived from animals, molds and bacteria, house dust (sand dust, fibrous particles, indoor dust such as mite feces) and the like can be specifically exemplified.

The allergen reduction means to reduce or render harmless the ability of the allergen itself to induce an allergic reaction, and animal allergens are clearly different from repellents. Specifically, for example, in an allergen measurement method by ELISA, under conditions treated with a 10-fold (weight ratio) agent with respect to mite extract (mite extract protein), Derfl (allergen derived from mite is used as a control for treatment with distilled water. When the amount of (protein) (control ratio) is 0.6 or less, more preferably 0.5 or less, still more preferably 0.4 or less, the allergen reducing effect can be obtained. The allergen-reducing effect of the sesquiterpene alcohol of the present invention is particularly effective against pet allergens such as mite allergen, house dust, cedar pollen allergen, and cat.

The sesquiterpene alcohol of the present invention comprises one or more of them, if necessary, as an emulsifier, a sticking agent, a dispersant,
By appropriately adding a wetting agent, a stabilizer, a propellant, etc.,
Oils, emulsions, wettable powders, sprays, aerosols, smoke agents,
It can be formulated in the form of coatings, detergents, shampoos, powders and granules. Specifically, residential cleaning agents, residential finishing agents, air conditioner filter cleaning agents, residential deodorants, air fresheners, residential bleaching agents, laundry detergents, softeners, pastes, clothing deodorants. , Clothing bleaching agents, cleaning paper products, kitchen detergents, kitchen bleaching agents, and the like. These are floor surfaces, tatami mats, carpets, duvets, carpets, tatami mats, walls, beds,
By spraying, spraying, coating or evaporating on a sofa, pillow or closet, washing clothes, curtains, treating the filter in an air purifier, or impregnating the gauze of a hay fever mask, or a futon. The effect can be exerted by treating the cloth of the cover, the sheet, and the pillow.

In addition to the sesquiterpene alcohol of the present invention, it is more effective to add a repellent against insects such as mites, moths, cockroaches, insecticides, etc. to the above-mentioned preparation,
Examples of such agents include insecticides, repellents, synergists, bactericides, antifungal agents, activators, deodorants and fragrances against mites, moths, cockroaches and the like.

For example, as an acaricide, d-phenothrin (3-phenoxybenzyl d-cis / trans-
Chrysanthemate), permethrin (3-phenoxybenzyl dl-cis / trans-2,2-dimethyl-3)
-(2 ', 2'-dichlorovinyl) -cyclopropanecarboxylate), resmethrin ((5-benzyl-3
-Furyl) methyl dl-cis / trans-chrysanthemate), allethrin (dl-3-allyl-2-methyl-4-oxo-2-cyclopentenyl dl-cis / trans-chrysanthemate), phthalthrin ((N −
3,4,5,6, -Tetrahydro-phthalimido) methyl dl-cis / trans-chrysanthate), empentrin (1-ethynyl-2-methyl-2-pentenyl dl-cis / trans-chrysanthate), d ，
Synthetic pyrethroids and derivatives thereof such as dT80-praletrin (d-2-methyl-4-oxo-3-propargylcyclopent-2-enyl d-cis / trans-chrysanthemate), hinokitiol, benzyl benzoate, Anti-mite substances derived from natural essential oil components such as jasmonic acid derivatives can be mentioned.

Examples of tick repellents include diethyl acid, dimethyl phthalate, dibutyl phthalate, MGK.
Repellent 326, Dovetrex, 2-Ethyl-
1,3-hexanediol or the like can be used.

Examples of the synergist and / or acaricide of acaricide include piperonyl butoxide, octachlorodipropyl ether, N- (2-ethylhexyl) -1-
Isopropyl-4-methylbicyclo [2,2,2] oct-5-ene-2,3-dicarboximide, N- (2
-Ethynyl) -bicyclo [2,2,1] -hepta-5-
En-2,3-dicarboximide and the like can be used.

Examples of fungicides and mildew-proofing agents that suppress the growth of fungi or bacteria that can feed indoor dust mites and have their own antigenicity include thiabendazole, triclosan, chlorhexidine, zinc pyrithione, chlorxylenol, densyl chloride. Examples include naturally occurring components such as benzalkonium, diclofluanid, sodium benzoate, methyl p-oxybenzoate, phenoxyethanol, ethanol and chitosan, catechin, thymol, hinokitiol, Moso bamboo extract, mustard essential oil, and wasabi essential oil.

The above-mentioned preparations include tannic acid, tea extract, hydroxyapatite, epicatechin, epigallocatechin gallate, epigallocatechin gallate, gallic acid, which are known as sesquiterpene alcohols of the present invention and known anti-allergen substances. (JP-A-6-279273), clay minerals such as smectite which is an allergen-scavenging substance, and a hydroxybenzoic acid compound known as an allergen-removing agent (JP-A-11-292714) and the like can be used in combination. .

The blending amount of the sesquiterpene alcohol of the present invention in the above-mentioned preparation can be appropriately determined according to the dosage form, treatment method, treatment location, etc., but the total amount of sesquiterpene alcohol in the entire composition is the same. , 0.001 to 20% by weight, more preferably 0.01 to 10.0% by weight, further preferably 0.01 to 3.0% by weight, and further 0.1. ~ 1.0 wt% or 0.5 ~
2.0% by weight is more preferable.

[0022]

EXAMPLES Example 1 Allergen measurement method by ELISA (1) 70% ethanol was used as a solvent so that the sesquiterpene alcohol of the present invention was a 1% solution. As the indoor environment allergen, the amount of Derfl, which is a leopard mite allergen, was measured by a sandwich ELISA method using a mouse monoclonal antibody using a mite f kit manufactured by LCD Allergy Laboratories, Inc. As a control, the allergen reduction effect of the 70% ethanol solution was measured by the same method. The allergen-reducing effect was expressed as a ratio to the control, with the amount of Derfl when the same treatment was performed using distilled water as 1.

(2) Tri-Scratch Extract "Dani"
(Torii Pharmaceutical Co., Ltd.) is put into a dialysis tube and 10% PBS
The solution was dialyzed overnight (4 ° C.) to remove glycerol contained in the extract. The concentration of this dialyzed mite extract is 1 mg
It was prepared with PBS so that it would be / mL. 50 μL of this mite extract and 50 μL of sample were put into a 1.5 mL siliconized microtube, and after stirring with vortex,
It was allowed to stand at room temperature for 2 hours. As a control, the same amount of distilled water was used instead of the sample. The same amount of 1% tannic acid was used as a positive control. Next, 11.25% BS
400 μL of A (dissolved in PBS) was added to each tube to stop the reaction, the mixture was centrifuged at 15,000 rpm at room temperature for 10 minutes, and the supernatant was subjected to ELISA. The amount of Derfl in the above reaction solution was measured using a mite f kit manufactured by LCD according to the attached protocol. D when using distilled water
Derfl treated with each sample with erfl amount of 1
The quantity ratio was determined. The results are shown in Table 1.

[0024]

[Table 1]

From the above, sesquiterpene alcohols such as farnesol, santalol, nerolidol, and cedrol had the same level of allergen reduction effect as tannic acid.

[0026]

By using the allergen reducing agent of the present invention, allergens such as house dust existing in the environment can be reduced without adversely affecting the human body and causing problems such as coloring.

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Kimihiko Hori             Kao Stock Exchange 2606, Akabane, Kai-cho, Haga-gun, Tochigi Prefecture             Company research institute F-term (reference) 4C206 AA01 AA02 CA03 CA08 CA09                       KA01 MA01 MA04 NA14 ZA34                       ZA59 ZB13

Claims (4)

[Claims] 1. An allergen reducing agent comprising a sesquiterpene alcohol. 2. The allergen reducing agent according to claim 1, wherein the allergen is selected from plant allergens, animal allergens, fungi, bacteria and house dust. 3. The allergen reducing agent according to claim 1, wherein the sesquiterpene alcohol is an acyclic sesquiterpene alcohol or a tricyclic sesquiterpene alcohol. 4. The allergen reducing agent according to claim 1, wherein the sesquiterpene alcohol is selected from cedrol, farnesol, santalol, and nerolidol.