JP2003137744A - Hair-restoring and growing agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a hair-restoring and growing agent which has excellent safety and scarcely irrigates skin. SOLUTION: This hair-restoring and growing agent contains the extraction liquid of Euglena cells as an active ingredient. The agent has not only its hair- restoring and growing effect, but has also high safety, scarce skin irrigation, excellent skin-improving effect and excellent moisture-retaining effect.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to improvement of hair condition,
In particular, the present invention relates to a hair-growth / hair-growth agent excellent in aging prevention and hair-growth / hair-growth effects.

[0002]

Conventionally, hair growth and nourishment containing carpronium chloride, minoxidil, pentadecanoic acid glyceride, etc., female hormones such as estradiol, topical stimulants such as capsicum tincture and cantharis tincture, and nutrients such as vitamins and amino acids. Hair cosmetics are manufactured and sold.

[0003] Vitamins and amino acids are expected to have an auxiliary role by nutritional supplementation, but they have no clear hair-growth and hair-growth effects.

[0004] Topical stimulants such as capsicum tincture and cantharis tincture have strong irritation to the skin and are not considered to be excellent in safety. In addition, female hormones such as carpronium chloride, minoxidil, pentadecanoic acid glyceride, and estradiol are known to have side effects, respectively, and cannot be said to be excellent in safety. There is a demand for a natural additive that is highly safe and has excellent hair-growth and hair-growth effects.

[0005]

Originally, cosmetics that are not irritating to the skin or have very low irritation and are excellent in safety and stability are required. In particular, there is a demand for a hair-growth / hair-growth agent that is excellent in hair-growth / hair-growth effects, has no irritation to the scalp, and is stable as a product. Therefore, an object of the present invention is to provide a hair growth / hair nourishing agent which has no or very low skin irritation, is excellent in safety and stability, and has a remarkable hair growth / hair nourishing effect.

[0006]

Means for Solving the Problems The present invention provides a cosmetic for hair-growth and hair-nourishing, which contains a Euglena cell extract as an active ingredient. In the present invention, the "Euglena microbial cell extract" refers to the Euglena intracellular solution obtained by extracting Euglena microbial cells and subjecting it to treatments such as centrifugation and ultrafiltration.
In the present invention, the "hair growth / hair nourishing agent" includes cosmetics provided for the purpose of hair growth / hair nourishing effect, quasi drugs, and pharmaceuticals, and not only those provided as "hair growth / hair nourishing agent", shampoo, Also included are those provided in the form of rinses, hair tonics, hair liquids, hair creams, hair mousses, hair blows, hair sprays and the like.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION The Euglena that can be used in the present invention refers to general protozoa belonging to Euglena. Euglena is widely distributed in freshwater such as ponds and swamps as algae, and these may be collected and used as they are. Alternatively, a mutant strain or variant obtained by mutating a naturally occurring protozoa belonging to these Euglena by a known method such as streptomycin treatment, ultraviolet treatment, or temperature treatment can also be used.
Euglena may be a strain having normal chloroplasts or a chloroplast-deficient strain. Particularly preferred Euglena include Euglena gracilis and mutants thereof, such as Euglena gracilis Z strain and SM-ZK strain. For these mutants and their obtaining methods, see, for example, Shozaburo Kitaoka, “Euglena-Physiology and Biochemistry”.
(Published by Academic Society Publishing Center Co., Ltd.), pages 238 to 239, and available from various research institutes.

In the present invention, although Euglena collected from nature may be used as it is, natural Euglena,
Alternatively, a Euglena mutant strain may be cultured and a cell extract may be prepared from the obtained cells. The medium used for culturing Euglena may be any medium used for ordinary culture, and may be a photoautotrophic medium, a photoheterotrophic medium, or a heterotrophic medium. Specifically, Cramer-Myers medium (Arch, Mikro
biol., 17,384-402 (1952)), Hutner medium (M
ethods Enzymol., 23, 143-162 (1971)), Koren-Hutner medium (J. Protozool., 14, Suppl., 1
7 (1967)) and the like are known.

Further, glucose is used as a carbon source in the medium,
Starch hydrolysates, molasses hydrolysates, glutamic acid, acetic acid, ethanol, mevalonic acid, lanosterol, ergosterol, farnesol, geraniol, various fatty acids (C3 to C18) and various fatty alcohols (C3 to C18), etc. are used. As the nitrogen source, use is made of inorganic nitrogen sources such as ammonium nitrate, ammonium sulfate, dibasic ammonium phosphate and ammonium hydrogen carbonate, amino acids such as glutamic acid, aspartic acid and arginine or organic nitrogen sources such as peptone, yeast extract and corn steve liquor. To be As the inorganic salt, inorganic salts such as potassium, magnesium, calcium, iron, manganese, cobalt, zinc, sodium, copper and nickel are used. In addition, micronutrients such as vitamin B1 and vitamin B12 are added and cultured, but other nutrient sources can be added if necessary. Except for mutant strains that do not have chlorophyll, such as the SM-ZK strain, it is also possible to culture Euglena by photosynthesis using a light-independent medium that does not use a carbon source.

Euglena is usually cultivated in a liquid medium by static culture, shaking culture, aeration stirring culture or the like, but it may be cultured under anaerobic conditions. After culturing under a suitable aerobic condition, culturing may be carried out under anaerobic conditions. Euglena is cultured at 20 to 30 ° C. The best growth is observed at 26 to 30 ° C. The pH of the medium depends on the composition of the medium, but pH2
8 and shows good growth at pH 2-5. Culturing is performed under either light irradiation or darkness, and the conditions of light irradiation and darkness are combined. 50 to 120 per minute during culture
It may be shaken once, but 90 to 105 per minute
The best growth is obtained by shaking once.

By culturing Euglena under the above conditions for 3 to 7 days, the stationary phase of growth is reached, and Euglena of up to about 30 g (wet weight) can be obtained per liter of the culture solution.

The Euglena cells used in the method of the present invention are cultivated in Euglena for the purpose of increasing the trehalose content in the cells, and the cultured cells are subjected to salt stress and / or osmotic stress. It may be placed. Here, "salt stress" refers to placing cells in an environment with a high salt concentration. The high concentration of salt causes inhibition of water absorption into cells, outflow of intracellular water to the outside, inhibition of substance metabolism by salts invading cells, and inhibition of absorption of other ions necessary for growth. The term "osmotic stress" refers to placing cells in a high-concentration environment mainly of soluble organic substances other than salts. As in the case of salt stress, inhibition of cell growth such as inhibition of water absorption into cells and outflow of water occurs.

As the applicants have shown in Japanese Patent Application Laid-Open No. 9-084593, when cultured bacterial cells are placed under such a stress environment, trehalose is synthesized from paramylon accumulated in the bacterial cells. It is considered that this is because Euglena produces trehalose from paramylon as a compatible solute for stress, accumulates in the cells and adapts to stress. Trehalose is a substance that has been conventionally blended as a moisturizing agent in cosmetics and the like, and a hair growth / hair nourishing agent having high moisturizing properties can be prepared by incorporating a bacterial cell extract having a high trehalose content.

Examples of the salt stress load substance used in such an embodiment of the present invention include sodium chloride, potassium chloride, magnesium chloride, calcium chloride, sodium sulfate, sodium phosphate and the like. Preferred is sodium chloride. For salt stress, cells are suspended in an appropriate buffer solution, and a stress-loading substance is added to the cells so that the concentration becomes a predetermined concentration. As the buffer solution, for example, a phosphate buffer solution is preferably used. Since the amount of trehalose synthesized increases depending on the salt concentration, the higher the salt concentration is, the more preferable. However, if the salt concentration is too high, the osmotic pressure around the cells will be too high and the Euglena cells will burst. In the method of the present invention, the preferred concentration of the salt stress load substance is about 200 mM.

Examples of the osmotic stress load substance include glucose, sucrose, fructose, lactose, dextrin, sorbitol, mannitol, glycerin and the like.
Particularly preferred are sorbitol and sucrose. Also in the case of osmotic stress, cells may be suspended in an appropriate buffer solution to dissolve a load substance having a predetermined concentration, as in the case of salt stress. Even when osmotic stress is applied, the amount of trehalose in cells increases in correlation with the concentration of the load substance, but it is necessary to make the concentration such that cells do not burst due to osmotic pressure. In the method of the present invention, the preferable concentration of the osmotic stress-loading substance is about 200 mM.

In the present invention, the time for which the Euglena is placed under stress conditions is 1 to 3 hours, preferably about 2 hours. The amount of trehalose produced reaches a substantially steady state by leaving it under a stress condition for about 2 hours.

In order to obtain an extract of Euglena cells, the cells may be recovered from the culture by centrifugation or the like, the cells may be disrupted by an appropriate method, and the contents inside the cells may be extracted. The method for extracting Euglena cells is not limited, and any known method is preferably used. For example, bacterial cells can be prepared from conventionally known purified water, ethanol, glycerin, 1,3
-A method of destroying cells by using butylene glycol or the like alone or in combination. As the liquid component used for extracting the microbial cells, if the one having the established safety to the human body, especially the skin is used, the microbial cell extract can be blended as it is. Further, physical force such as ultrasonic treatment or mashing of cells may be used in combination for cell extraction. After the extraction operation, cell debris can be removed from the extract by a known means such as filtration or centrifugation to obtain a cell extract.

The safety of the bacterial cell extract thus obtained has already been established, and the extract may be blended as it is with a hair-growing / hair-growing agent.

The amount of the Euglena cell extract to be incorporated in the hair-restoring and hair-restoring agent of the present invention is not particularly limited, and the type of Euglena used, the culturing method, the extracting method, and the hair-growing /
It changes depending on the application target of the hair-growth agent and the required strength of hair-growth and hair-growth effects. Therefore, when a hair growth / hair nourishing agent is prepared using the Euglena cell extract obtained under new conditions, the amount to be blended must be appropriately determined so as to obtain the desired effects. The determination of such blending amount is within the ordinary work of those skilled in the art.

The euglena cell extract has less irritation to the skin and is excellent not only in hair growth and hair nourishing effects but also in skin improving effects. In addition, Euglena bacterial cells, including moisturizing ingredients,
It is known that various skin contains good ingredients,
It is possible to prepare a hair growth and nourishing agent that is gentle on the skin.

In the hair restorer / hair nourishing agent of the present invention, the components other than the Euglena cell extract are not particularly limited, and any of those usually used in the production of cosmetics are preferably used.

Compounding ingredients other than Euglena cell extract include squalane, liquid paraffin, α as an oily base.
Hydrocarbons such as olefin oligomers, paraffin wax, ceresin, microcrystalline wax, corn oil, soybean oil, rapeseed oil, sunflower oil, safflower oil, avocado oil, olive oil, coconut oil, beef tallow, pork oil, mink oil, etc. It may contain animal and vegetable oils, synthetic esters such as isopropyl myristate, cetyl octoate, cetyl palmitate, etc., natural animal and vegetable waxes such as jojoba oil, carnauba wax, candelilla wax, oak and beeswax. Further, glyceryl stearate, sorbitan stearate, polyoxyethylene sorbitan monooleate, polyoxyethylene glyceryl tristearate, polyoxyethylene lauryl ether, decaglyceryl trioleate, sucrose monolaurate,
Surfactants such as polyoxyethylene hydrogenated castor oil, silicone oil or its derivatives such as dimethylpolysiloxane, methylphenylpolysiloxane, fluororesins such as perfluoropolyether, ethanol, isopropanol,
Alcohols such as ethylene glycol, glycerin, 1,3-butylene glycol, propylene glycol, diglycerin, carboxyvinyl polymer, carrageenan,
Water-soluble polymers such as xanthan gum, sodium carboxymethylcellulose, sodium hyaluronate, collagen, elastin, silk, lactoferrin and other proteins and their hydrolysates, titanium dioxide, zinc oxide, talc, mica, silicic anhydride, nylon powder, polyacrylic Alkylate, alumina, powder of iron oxide, etc., as well as UV absorbers, vitamins, anti-inflammatory agents, amino acids and their derivatives, lecithin, colorants, fragrances, preservatives, etc. It is possible.

The hair-growing / hair-growing agents of the present invention include carpronium chloride, minoxidil, glyceride pentadecanoic acid glycerides, female hormones such as estradiol, and local stimulants such as capsicum tincture and cantharisin tincture. You may add the component used.

The method for producing the hair restorer / hair nourishing agent of the present invention is not particularly limited, and a conventionally known method for producing a hair restorer / hair nourishing agent is appropriately adopted according to the formulation, and Euglena cell extract at an appropriate time point. Can be added to prepare.

EXAMPLES The present invention will now be specifically described with reference to examples, but the present invention is not limited to the examples.

Preparation Example 1 The Euglena gracilis SM-ZK strain was subjected to shaking culture (90 to 120 revolutions / minute) at 30 ° C. for 4 days in a Koren-Hattner medium. Centrifugation after culture (500G,
Euglena cells were obtained after 3 minutes. Purified water in an amount equal to the wet weight of Euglena cells was added, and the cells were disrupted using a disper and then centrifuged (3000 G, 3 minutes) to remove cell debris to obtain an Euglena cell extract.

When this Euglena cell extract was subjected to general human patch test, eye irritation test, primary skin irritation test, sensitization test, and sudden reversion mutagenicity test, no irritation was observed. It was confirmed that it is safe as a cosmetic raw material.

The Euglena cell extract obtained in Preparation Example 1 was used to prepare a hair-growth / hair-growth tonic according to the formulation shown in Table 1, and their effects were compared.

[0029]

[Table 1] Preparation method of hair-growth and hair-recovery tonic: Mix all components at room temperature and filter.

Evaluation method 1 A hair growth / hair nourishing effect evaluation test in C3H mice was carried out. The obtained hair-growth / hair-growth tonics of Example 1 and Comparative Example 1 were continuously transdermally administered to 8-week-old C3H mice with depilated back for 21 days, and the change in skin color at the administration site and hair extension were observed. The degree was scored and evaluated based on the area. The respective evaluation criteria are shown in Tables 2 and 3. Furthermore, a total score (comprehensive evaluation) of two items was calculated.

Evaluation criteria

[Table 2]

[0032]

[Table 3]

The results are shown in Table 4.

[Table 4]

As shown in Table 4 above, the change in skin color,
There was no difference in the number of days until hair elongation was observed between Example 1 and Comparative Example 1. However, the score evaluated based on the area where each change was observed and the total score of the two items (comprehensive evaluation) were higher in Example 1 than in Comparative Example 1, and in Example 1, hair growth and nourishment was performed over a wider range. Hair effect was observed.

Evaluation Method 2 The feeling of use of the hair-growth / hair-growth tonics of Preparation Example 1 and Comparative Example 1 was evaluated using an in-house panel. Five in-house panels used the above-mentioned hair-growth and hair-nourishing tonic in the usual way, and had one point in the hair-growth and hair-growth effect, refreshing feeling, irritation, moistness, and dry state after use, with a slight defect Two
It was done by assigning points, 3 for normal, 4 for good, and 5 for good. The results are shown in Table 5. The results are shown by the average value of the evaluation by 5 panelists.

[0036]

[Table 5]

Preparation Example 2 Euglena gracilis strain Z was shake-cultured at 90 ° C. in a Koren-Hattner medium at 90 to 120 rpm.
Minutes) for 4 days. Centrifugation after culture (500G, 3
Euglena cells were obtained by This bacterium is 15 mM
It was washed twice with the phosphate buffer solution (pH 6.8). Further, the suspension was suspended in the same solution, NaCl was added to 200 mM, and the mixture was allowed to stand at 26 ° C. for 2 hours and then treated with salt to recover the bacterial cells. Purified water in the same amount as the wet weight of the cells was added, and the cells were disrupted using a disper and centrifuged (3000 G, 3 minutes) to obtain a Euglena cell extract.

By the salt-loading treatment, trehalose was accumulated in the Euglena cell extract, and a Euglena cell extract having more excellent moisturizing property was obtained.

With respect to the Euglena cell extract obtained in Preparative Example 2, a hair-growth / hair-growth tonic of Example 2 was produced in the same formulation as in Preparative Example 1. When this was subjected to a usability test by a panel of 5 persons in the same manner as in Evaluation Method 2, the evaluation of items other than the moist feeling did not change, and only the moist feeling increased to 4.8 points and the evaluation point increased. It was

EFFECTS OF THE INVENTION A hair-growth / hair-growth agent containing the Euglena cell extract of the present invention has high safety in addition to its hair-growth / hair-growth effect,
It is an extremely useful hair growth and nourishing agent that has less irritation to the skin and has excellent skin improving and moisturizing effects.

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Takatsugu Yoshioka             1-7-11 Doshomachi, Chuo-ku, Osaka-shi, Osaka Prefecture               Iwase Cosfa Co., Ltd. F-term (reference) 4C083 AA031 AA032 AC102 AC122                       AC172 AD532 CC37 DD27                       EE22                 4C088 AA12 AA15 BA09 CA05 NA05                       NA06 NA07 ZA92

Claims (3)

[Claims] 1. A hair restorer / hair nourishing agent, which contains an extract of Euglena cells as an active ingredient. 2. The hair-growth / hair-restoration agent according to claim 1, wherein the Euglena cells are selected from the group consisting of wild-type, Euglena Z strain, and SM-ZK strain. 3. A Euglena cell extract is obtained by culturing Euglena, subjecting the cultured cells to salt stress and / or osmotic stress, and then extracting Euglena cells. The hair restorer / hair nourishing agent according to claim 1 or 2.