JP2003092997A - Method for treating sharkskin and method for obtaining collagen - Google Patents
Method for treating sharkskin and method for obtaining collagenInfo
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- JP2003092997A JP2003092997A JP2001290201A JP2001290201A JP2003092997A JP 2003092997 A JP2003092997 A JP 2003092997A JP 2001290201 A JP2001290201 A JP 2001290201A JP 2001290201 A JP2001290201 A JP 2001290201A JP 2003092997 A JP2003092997 A JP 2003092997A
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- shark skin
- collagen
- sharkskin
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Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、サメ皮から鱗を除
去するのに適したサメ皮の処理方法およびサメ皮からコ
ラーゲンを得るためのコラーゲン製造方法に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for treating shark skin suitable for removing scales from shark skin and a method for producing collagen for obtaining collagen from shark skin.
【０００２】[0002]
【従来の技術】近年、コラーゲンが美容や健康などへの
効果から着目されている。従来、コラーゲンは、牛、豚
等の動物から抽出するのが一般的であるが、魚、特にサ
メ皮の真皮にコラーゲンが多量に含まれていることが知
られている。サメ皮からコラーゲンを抽出する場合、皮
に鱗が付いた状態で抽出を行っている。しかしながら、
皮に鱗が付いた状態で抽出を行うと、コラーゲンに不純
物が混入し、純粋なコラーゲンが抽出できないという課
題があった。2. Description of the Related Art In recent years, collagen has attracted attention because of its effects on beauty and health. Conventionally, collagen is generally extracted from animals such as cows and pigs, but it is known that a large amount of collagen is contained in the dermis of fish, especially shark skin. When extracting collagen from shark skin, it is extracted with scales on the skin. However,
When extraction is carried out with the scales on the skin, there is a problem that impurities are mixed in collagen and pure collagen cannot be extracted.
【０００３】その課題を解決するため、コラーゲン抽出
の前処理として、サメ皮から鱗を除去することが望まれ
る。従来、サメ皮から鱗を除去する方法として、サメ皮
を60℃〜70℃の湯で煮る方法がある。しかしながら、サ
メ皮を煮た場合、コラーゲンは30℃以上の温度で加熱す
ると、変性してしまい、純粋なコラーゲンを抽出するこ
とができないという課題があった。In order to solve the problem, it is desired to remove scales from shark skin as a pretreatment for collagen extraction. Conventionally, as a method of removing scales from shark skin, there is a method of boiling shark skin in hot water at 60 ° C to 70 ° C. However, when shark skin was boiled, collagen was denatured when heated at a temperature of 30 ° C. or higher, and pure collagen could not be extracted.
【０００４】[0004]
【発明が解決しようとする課題】本発明は、このような
従来の課題に着目してなされたもので、コラーゲンを変
性させずにサメ皮から鱗を除去するのに適したサメ皮の
処理方法およびサメ皮から純度が高いコラーゲンを得る
ためのコラーゲン製造方法に関する。SUMMARY OF THE INVENTION The present invention has been made in view of such conventional problems, and is a method for treating shark skin suitable for removing scales from shark skin without denaturing collagen. And a method for producing collagen for obtaining highly pure collagen from shark skin.
【０００５】[0005]
【課題を解決するための手段】上記目的を達成するため
に、本発明に係るサメ皮の処理方法は、サメ皮を硫化ナ
トリウムまたは水酸化ナトリウムで処理する第１工程
と、第１工程後のサメ皮を消石灰で処理する第２工程
と、第２工程後のサメ皮を水洗いする第３工程と、第３
工程後のサメ皮を硫酸アンモニウムおよび塩化ナトリウ
ムで処理する第４工程と、第４工程後のサメ皮を塩酸ま
たは酢酸および塩化ナトリウムの水溶液に浸漬する第５
工程と、第５工程後のサメ皮を水洗いする第６工程と、
第６工程後のサメ皮を水に浸漬する第７工程とを、有す
ることを特徴とする。In order to achieve the above object, a method for treating shark skin according to the present invention comprises a first step of treating shark skin with sodium sulfide or sodium hydroxide, and a step after the first step. A second step of treating the shark skin with slaked lime, a third step of washing the shark skin after the second step with water, and a third step
The fourth step of treating the shark skin after the step with ammonium sulfate and sodium chloride, and the fifth step of immersing the shark skin after the fourth step in an aqueous solution of hydrochloric acid or acetic acid and sodium chloride.
And a sixth step of washing the shark skin after the fifth step with water,
And a seventh step of immersing the shark skin after the sixth step in water.
【０００６】本発明に係るコラーゲン製造方法は、サメ
皮を硫化ナトリウムまたは水酸化ナトリウムで処理する
第１工程と、第１工程後のサメ皮を消石灰で処理する第
２工程と、第２工程後のサメ皮を水洗いする第３工程
と、第３工程後のサメ皮を硫酸アンモニウムおよび塩化
ナトリウムで処理する第４工程と、第４工程後のサメ皮
を塩酸または酢酸および塩化ナトリウムの水溶液に浸漬
する第５工程と、第５工程後のサメ皮を水洗いする第６
工程と、第６工程後のサメ皮を水に浸漬する第７工程
と、第７工程後のサメ皮から鱗を除去する第８工程と、
第８工程後のサメ皮からコラーゲンを抽出する第９工程
とを、有することを特徴とする。The method for producing collagen according to the present invention comprises a first step of treating shark skin with sodium sulfide or sodium hydroxide, a second step of treating shark skin with slaked lime after the first step, and a second step. 3rd step of washing the shark skin with water, a 4th step of treating the shark skin after the 3rd step with ammonium sulfate and sodium chloride, and immersing the shark skin after the 4th step in an aqueous solution of hydrochloric acid or acetic acid and sodium chloride The fifth step and the sixth step of washing the shark skin after the fifth step
A step, a seventh step of immersing the shark skin after the sixth step in water, and an eighth step of removing scales from the shark skin after the seventh step,
And a ninth step of extracting collagen from the shark skin after the eighth step.
【０００７】本発明において、サメ皮には、ヨシキリザ
メその他のサメの皮を用いることができる。なお、本明
細書中で、「サメ皮」は、サメの鰭を含む概念である。
第１工程の処理は、サメ皮を硫化ナトリウムまたは水酸
化ナトリウムの水溶液に浸漬して行っても、その粉末で
覆って行ってもよい。第１工程の処理時間は、20分〜１
時間30分位が好ましい。硫化ナトリウムに浸漬する場合
の濃度は、水１リットルあたり８ｇ乃至１５ｇが好まし
い。水酸化ナトリウムに浸漬する場合の濃度は、水１リ
ットルあたり８ｇ乃至１８ｇが好ましい。In the present invention, as the shark skin, a blue shark or other shark skin can be used. In the present specification, "shark skin" is a concept including shark fins.
The treatment of the first step may be performed by immersing the shark skin in an aqueous solution of sodium sulfide or sodium hydroxide, or by covering it with its powder. The processing time of the first step is 20 minutes to 1
About 30 minutes is preferable. When immersed in sodium sulfide, the concentration is preferably 8 to 15 g per liter of water. When immersed in sodium hydroxide, the concentration is preferably 8 to 18 g per liter of water.
【０００８】第２工程の処理は、サメ皮を浸漬した水溶
液に消石灰を入れて行っても、サメ皮を消石灰の粉末で
覆って行ってもよい。第２工程の処理時間は、20分〜１
時間30分位が好ましい。消石灰に浸漬する場合の濃度
は、水１リットルあたり５０ｇ乃至１６０ｇが好まし
い。第４工程の処理は、サメ皮を硫酸アンモニウムおよ
び塩化ナトリウムの水溶液に浸漬して行っても、それら
の混合粉末で覆って行ってもよい。第４工程の処理時間
は、１時間前後が好ましい。浸漬する場合の濃度は、水
１リットルあたり硫酸アンモニウムが６ｇ乃至１３ｇ、
塩化ナトリウムが８ｇ乃至１８ｇが好ましい。The treatment of the second step may be carried out by adding slaked lime to the aqueous solution in which the shark skin is immersed, or by covering the shark skin with a powder of slaked lime. The processing time of the second step is 20 minutes to 1
About 30 minutes is preferable. When immersed in slaked lime, the concentration is preferably 50 g to 160 g per liter of water. The treatment of the fourth step may be performed by immersing the shark skin in an aqueous solution of ammonium sulfate and sodium chloride, or by covering it with a mixed powder thereof. The treatment time of the fourth step is preferably about 1 hour. When soaking, the concentration of ammonium sulfate is 6 to 13 g per liter of water,
Sodium chloride is preferably 8 g to 18 g.
【０００９】第５工程の処理時間は、30分〜２時間位が
好ましい。第５工程の水溶液の濃度は、水１リットルあ
たり１Ｎ塩酸の場合、７０ｇ乃至１５０ｇ、酢酸の場
合、１５０ｇ乃至２５０ｇ、塩化ナトリウムが８ｇ乃至
１８ｇが好ましい。第７工程の処理時間は、一昼夜が好
ましい。The treatment time of the fifth step is preferably about 30 minutes to 2 hours. The concentration of the aqueous solution in the fifth step is preferably 70 g to 150 g in the case of 1N hydrochloric acid, 150 g to 250 g in the case of acetic acid, and 8 g to 18 g of sodium chloride per liter of water. The processing time of the seventh step is preferably one day and one night.
【００１０】本発明に係るサメ皮の処理方法によれば、
第７工程後のサメ皮は、鱗を容易に除去できるものであ
って、さらに表皮と真皮とに容易に分離することができ
る。分離した真皮は、コラーゲン含量が高く、コラーゲ
ンの材料として適している。本発明に係るサメ皮の処理
方法は、加熱処理を行わないため、コラーゲンを変性さ
せずにサメ皮から鱗を除去する前処理として適してい
る。According to the shark skin treatment method of the present invention,
The shark skin after the 7th step can easily remove scales, and can be easily separated into the epidermis and the dermis. The separated dermis has a high collagen content and is suitable as a collagen material. Since the shark skin treatment method according to the present invention does not perform heat treatment, it is suitable as a pretreatment for removing scales from the shark skin without denaturing collagen.
【００１１】本発明に係るコラーゲン製造方法によれ
ば、第１工程乃至第７工程の処理によりサメ皮は鱗を容
易に除去できるものとなり、さらに表皮と真皮とに容易
に分離できるものとなる。第１工程乃至第８工程の処理
では加熱処理を行わないため、第８工程でコラーゲンを
変性させずにサメ皮から鱗を除去することができる。第
９工程でサメ皮から抽出したコラーゲンは、臭気が除去
され、純度が高いものが抽出される。なお、第９工程で
は、サメ皮から真皮を分離し、分離した真皮からコラー
ゲンを抽出することが好ましい。分離した真皮は、コラ
ーゲン含量が高いためである。しかしながら、第９工程
は、サメ皮から真皮を分離するだけの処理から成っても
よい。According to the method for producing collagen of the present invention, the scales of the shark skin can be easily removed by the treatments of the first to seventh steps, and the epidermis and the dermis can be easily separated. Since heat treatment is not performed in the treatments of the first to eighth steps, scales can be removed from the shark skin without denaturing collagen in the eighth step. The odor is removed from the collagen extracted from the shark skin in the ninth step, and the highly pure collagen is extracted. In the ninth step, it is preferable to separate the dermis from the shark skin and extract collagen from the separated dermis. This is because the separated dermis has a high collagen content. However, the ninth step may consist of just separating the dermis from the shark skin.
【００１２】本発明に係るサメ皮の処理方法の後に鱗を
除去されたサメ皮および本発明に係るコラーゲン製造方
法により製造されたコラーゲンは、食品、化粧品、医薬
品またはそれらの材料として用いることができる。The shark skin from which scales have been removed after the method for treating shark skin according to the present invention and the collagen produced by the method for producing collagen according to the present invention can be used as foods, cosmetics, pharmaceuticals or materials thereof. .
【００１３】[0013]
【実施例】以下、本発明の実施例について説明する。ヨ
シキリザメを解凍し、生皮又は生鰭を切り取った。その
切り取ったサメ皮１ｋｇを、水１リットルあたり４０ｇ
の硫化ナトリウムを溶解させた水溶液１リットルに１時
間、浸漬した。その後、その水溶液中に水１リットルあ
たり６０ｇの消石灰を投入し、攪拌して１時間、浸漬し
た。その後、水溶液からサメ皮を取り出し、十分に水洗
いした。EXAMPLES Examples of the present invention will be described below. The blue shark was thawed and the raw hides or fins were cut off. 1 kg of the cut shark skin is 40 g per 1 liter of water
It was immersed for 1 hour in 1 liter of an aqueous solution containing sodium sulfide dissolved therein. Then, 60 g of slaked lime per 1 liter of water was added to the aqueous solution, and the mixture was stirred and immersed for 1 hour. Then, the shark skin was taken out of the aqueous solution and thoroughly washed with water.
【００１４】水洗い後のサメ皮を、水１リットルあたり
４０ｇの硫酸アンモニウムおよび４０ｇの塩化ナトリウ
ムを溶解させた水溶液１リットルに１時間、浸漬した。
その後、水溶液からサメ皮を取り出し、十分に水洗いし
た。水洗い後のサメ皮を、水１リットルあたり３００ｇ
の１Ｎ塩酸および１００ｇの塩化ナトリウムを溶解させ
た水溶液１リットルに１時間、浸漬した。その後、水溶
液からサメ皮を取り出し、十分に水洗いした。After washing with water, the shark skin was immersed in 1 liter of an aqueous solution in which 40 g of ammonium sulfate and 40 g of sodium chloride were dissolved in 1 liter of water for 1 hour.
Then, the shark skin was taken out of the aqueous solution and thoroughly washed with water. 300g of shark skin after washing with water per liter of water
1 N hydrochloric acid and 100 g of sodium chloride were dissolved in 1 liter of an aqueous solution for 1 hour. Then, the shark skin was taken out of the aqueous solution and thoroughly washed with water.
【００１５】水洗い後のサメ皮を１昼夜、水槽に入れ、
水戻しをした。翌日、水槽から取り出したサメ皮の表面
をこすって、鱗を除去した。こうして、加熱処理を行わ
ずに、サメ皮から鱗を２０℃の常温で容易に除去するこ
とができた。鱗を除去したサメ皮から真皮を剥がし、剥
がした真皮から常法に従ってコラーゲンを抽出した。コ
ラーゲンは真皮に多く含まれているが、前述の処理によ
り、サメ皮は表皮と真皮とウロコとに容易に分けられる
ようになる。抽出したコラーゲンは、変性を起こしてお
らず、臭気が除去され、純度が高いものであった。な
お、除去した鱗もまた、未変成のコラーゲンの材料とし
て利用することができる。After washing the shark skin, put it in the aquarium for one day
I rehydrated. The next day, the scales were removed by rubbing the surface of the shark skin removed from the water tank. Thus, scales could be easily removed from the shark skin at room temperature of 20 ° C. without heat treatment. The dermis was peeled from the shark skin from which the scale had been removed, and collagen was extracted from the peeled dermis according to a conventional method. Although a large amount of collagen is contained in the dermis, the shark skin can be easily divided into the epidermis, the dermis, and the scale by the above-mentioned treatment. The extracted collagen did not undergo denaturation, its odor was removed, and its purity was high. The removed scale can also be used as a material for unmodified collagen.
【００１６】[0016]
【実験】（サメ皮の分析）実験は、前記実施例により20
℃で脱鱗したサメ皮（20℃処理皮）と、67℃で脱鱗した
サメ皮（67℃処理皮）について行った。実験は、示差走
査熱量測定（DSC）および比旋光度測定によりコラーゲ
ンの変性・未変性状態を推定することを目的として行っ
た。[Experiment] (Analysis of shark skin)
It carried out about the shark skin descaled at 20 degreeC (20 degreeC treated skin) and the shark skin descaled at 67 degreeC (67 degreeC treated skin). The experiment was carried out for the purpose of estimating the denatured / undenatured state of collagen by differential scanning calorimetry (DSC) and specific optical rotation measurement.
【００１７】（実験方法−示差走査熱量測定（DSC））
提供試料のサメ皮を軽く水洗後、湿重量として約50mg採
取し、70μｌ容の銀製試料セルに密封して、DSC測定
（装置の商品名：ＳＳＣ5200、DSC120、セイコー電子工
業）を行った。測定条件は、昇温速度：2℃／min、リフ
ァレンス：水、炉内雰囲気：ヘリウムガス（流量40ml／
min）で行った。(Experimental Method-Differential Scanning Calorimetry (DSC))
After gently washing the shark skin of the provided sample with water, about 50 mg as a wet weight was sampled and sealed in a silver sample cell having a volume of 70 μl, and DSC measurement (device brand name: SSC5200, DSC120, Seiko Instruments Inc.) was performed. The measurement conditions are: temperature rising rate: 2 ° C / min, reference: water, furnace atmosphere: helium gas (flow rate 40 ml /
min).
【００１８】得られたDSC曲線から、低温側のベースラ
インと吸熱曲線の最大傾斜点を通る接線との交点を熱変
性温度として読み取った。また、ベースラインと吸熱曲
線で囲まれた面積から熱変性温度に伴う吸熱エネルギー
（熱変性エンタルピー、ΔH）を求めた。From the obtained DSC curve, the intersection of the low temperature side baseline and the tangent line passing through the maximum slope of the endothermic curve was read as the heat denaturation temperature. Also, the endothermic energy (heat denaturation enthalpy, ΔH) accompanying the heat denaturation temperature was determined from the area surrounded by the baseline and the endothermic curve.
【００１９】（実験方法−比旋光度の測定）比旋光度測
定用のコラーゲン溶液を調製し、調製した試料溶液を比
旋光度の測定に供した。測定は、旋光計（商品名：日本
分光（株）旋光計ＤＩＰ−１０００型）を用いて、１０
ｃｍセルで、温度１０℃および３５℃、波長５８９ｎｍ
で行った。(Experimental Method-Measurement of Specific Optical Rotation) A collagen solution for measuring specific optical rotation was prepared, and the prepared sample solution was subjected to measurement of specific optical rotation. The measurement was performed using a polarimeter (trade name: JASCO Corporation polarimeter DIP-1000 type) for 10
cm cell, temperature 10 ° C and 35 ° C, wavelength 589 nm
I went there.
【００２０】ＤＳＣにより測定した熱変性温度と吸熱エ
ネルギー量の結果を表１に示す。比旋光度の結果を表２
に示す。また、水分、全灰分、皮質分の測定結果は、以
下のとおりである。水分（ＪＩＳ Ｋ ６５５０に基づ
く試験）は２０℃処理皮で９５．６％、６７℃処理皮で
８０．３％、全灰分（無水物換算、ＪＩＳ Ｋ ６５５
０に基づく試験）は２０℃処理皮で０．５％、６７℃処
理皮で１．２％、皮質分（無水物換算、但し、全自動窒
素分析装置で窒素分を測定し、タンパク質含有量として
換算した（換算係数５．６２））は２０℃処理皮で９
５．２％、６７℃処理皮で９６．５％であった。Table 1 shows the results of the heat denaturation temperature and the amount of endothermic energy measured by DSC. Table 2 shows the results of specific rotation
Shown in. The measurement results of water content, total ash content, and cortical content are as follows. Moisture (test based on JIS K 6550) is 95.6% for 20 ° C. treated skin, 80.3% for 67 ° C. treated skin, total ash (anhydrous equivalent, JIS K 655
Test based on 0) is 0.5% for 20 ° C. treated skin, 1.2% for 67 ° C. treated skin, cortical content (anhydrous equivalent, but nitrogen content was measured with a fully automatic nitrogen analyzer, protein content Is converted as (conversion coefficient 5.62) is 9 at 20 ° C treated leather.
It was 5.2%, and it was 96.5% in the 67 ° C treated leather.
【００２１】[0021]
【表１】 [Table 1]
【００２２】[0022]
【表２】 [Table 2]
【００２３】ＤＳＣと比旋光度の結果から、２０℃処理
皮は未変性状態であることが推定される。From the results of DSC and specific optical rotation, it is estimated that the skin treated at 20 ° C. is in a non-denatured state.
【００２４】[0024]
【発明の効果】本発明によれば、コラーゲンを変性させ
ずにサメ皮から鱗を除去するのに適したサメ皮の処理方
法およびサメ皮から純度が高いコラーゲンを得るための
コラーゲン製造方法を提供することができる。According to the present invention, a method for treating shark skin suitable for removing scales from shark skin without denaturing collagen and a method for producing collagen for obtaining highly pure collagen from shark skin are provided. can do.
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【手続補正書】[Procedure amendment]
【提出日】平成１３年９月２５日（２００１．９．２
５）[Submission date] September 25, 2001 (2001.9.2)
5)
【手続補正１】[Procedure Amendment 1]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】００１５[Name of item to be corrected] 0015
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【００１５】水洗い後のサメ皮を１昼夜、水槽に入れ、
水戻しをした。翌日、水槽から取り出したサメ皮の表面
をこすって、鱗を除去した。こうして、加熱処理を行わ
ずに、サメ皮から鱗を２０℃の常温で容易に除去するこ
とができた。鱗を除去したサメ皮から真皮を剥がし、剥
がした真皮から常法に従ってコラーゲンを抽出した。コ
ラーゲンは真皮に多く含まれているが、前述の処理によ
り、サメ皮は表皮と真皮とウロコとに容易に分けられる
ようになる。抽出したコラーゲンは、変性を起こしてお
らず、臭気が除去され、純度が高いものであった。な
お、除去した鱗もまた、未変性のコラーゲンの材料とし
て利用することができる。After washing the shark skin, put it in the aquarium for one day
I rehydrated. The next day, the scales were removed by rubbing the surface of the shark skin removed from the water tank. Thus, scales could be easily removed from the shark skin at room temperature of 20 ° C. without heat treatment. The dermis was peeled from the shark skin from which the scale had been removed, and collagen was extracted from the peeled dermis according to a conventional method. Although a large amount of collagen is contained in the dermis, the shark skin can be easily divided into the epidermis, the dermis, and the scale by the above-mentioned treatment. The extracted collagen did not undergo denaturation, its odor was removed, and its purity was high. The removed scale can also be used as a material for the undenatured collagen.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.7 識別記号 ＦＩ テーマコート゛(参考） Ａ６１Ｋ 7/48 Ａ６１Ｋ 7/48 ─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 7 Identification code FI theme code (reference) A61K 7/48 A61K 7/48
Claims (2)
リウムで処理する第１工程と、 第１工程後のサメ皮を消石灰で処理する第２工程と、 第２工程後のサメ皮を水洗いする第３工程と、 第３工程後のサメ皮を硫酸アンモニウムおよび塩化ナト
リウムで処理する第４工程と、 第４工程後のサメ皮を塩酸または酢酸および塩化ナトリ
ウムの水溶液に浸漬する第５工程と、 第５工程後のサメ皮を水洗いする第６工程と、 第６工程後のサメ皮を水に浸漬する第７工程とを、 有することを特徴とするサメ皮の処理方法。1. A first step of treating shark skin with sodium sulfide or sodium hydroxide, a second step of treating shark skin with slaked lime after the first step, and a second step of washing shark skin with water after the second step. 3 steps, 4th step of treating the shark skin after the 3rd step with ammonium sulfate and sodium chloride, 5th step of immersing the shark skin after the 4th step in an aqueous solution of hydrochloric acid or acetic acid and sodium chloride, 5th step A method for treating shark skin, comprising a sixth step of washing the shark skin after the step with water and a seventh step of immersing the shark skin after the sixth step in water.
リウムで処理する第１工程と、 第１工程後のサメ皮を消石灰で処理する第２工程と、 第２工程後のサメ皮を水洗いする第３工程と、 第３工程後のサメ皮を硫酸アンモニウムおよび塩化ナト
リウムで処理する第４工程と、 第４工程後のサメ皮を塩酸または酢酸および塩化ナトリ
ウムの水溶液に浸漬する第５工程と、 第５工程後のサメ皮を水洗いする第６工程と、 第６工程後のサメ皮を水に浸漬する第７工程と、 第７工程後のサメ皮から鱗を除去する第８工程と、 第８工程後のサメ皮からコラーゲンを抽出する第９工程
とを、 有することを特徴とするコラーゲン製造方法。2. A first step of treating shark skin with sodium sulfide or sodium hydroxide, a second step of treating shark skin with slaked lime after the first step, and a second step of washing shark skin with water after the second step. 3 steps, 4th step of treating the shark skin after the 3rd step with ammonium sulfate and sodium chloride, 5th step of immersing the shark skin after the 4th step in an aqueous solution of hydrochloric acid or acetic acid and sodium chloride, 5th step A sixth step of washing the shark skin after the step with water, a seventh step of immersing the shark skin after the sixth step in water, an eighth step of removing scales from the shark skin after the seventh step, and an eighth step A ninth step of extracting collagen from the shark skin afterwards.
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| JP2001290201A JP3616046B2 (en) | 2001-09-21 | 2001-09-21 | Method for treating shark skin and method for producing collagen |
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| JP2003092997A true JP2003092997A (en) | 2003-04-02 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006180744A (en) * | 2004-12-27 | 2006-07-13 | Yaizu Suisankagaku Industry Co Ltd | Method for cleaning fishskin |
| JP2007211053A (en) * | 2006-02-07 | 2007-08-23 | Miyagi Prefecture | Blue dye, blue collagen or gelatin and method for producing those |
| KR101100411B1 (en) | 2008-12-24 | 2011-12-30 | 대구가톨릭대학교산학협력단 | Curd Type Dombaeki Soup and Method for preparing the same |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03209395A (en) * | 1988-08-04 | 1991-09-12 | Univ Bar Ilan | Production of gelatin from fish skin |
| JPH05155900A (en) * | 1991-09-30 | 1993-06-22 | Nippon Kasei Chem Co Ltd | High-purity acid-insoluble fish scale collagen and its production |
| JPH05179300A (en) * | 1992-01-06 | 1993-07-20 | Kitano Kagaku:Kk | Production of reptile leather |
| JPH10276680A (en) * | 1997-04-04 | 1998-10-20 | Ihara Suisan Kk | Production of fish gelatin |
| JP2000050811A (en) * | 1998-08-11 | 2000-02-22 | Ihara Suisan Kk | Production of collagen of fishes |
| JP2000189065A (en) * | 1998-12-31 | 2000-07-11 | Skw Biosystems | Production of fish gelatin |
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2001
- 2001-09-21 JP JP2001290201A patent/JP3616046B2/en not_active Expired - Lifetime
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03209395A (en) * | 1988-08-04 | 1991-09-12 | Univ Bar Ilan | Production of gelatin from fish skin |
| JPH05155900A (en) * | 1991-09-30 | 1993-06-22 | Nippon Kasei Chem Co Ltd | High-purity acid-insoluble fish scale collagen and its production |
| JPH05179300A (en) * | 1992-01-06 | 1993-07-20 | Kitano Kagaku:Kk | Production of reptile leather |
| JPH10276680A (en) * | 1997-04-04 | 1998-10-20 | Ihara Suisan Kk | Production of fish gelatin |
| JP2000050811A (en) * | 1998-08-11 | 2000-02-22 | Ihara Suisan Kk | Production of collagen of fishes |
| JP2000189065A (en) * | 1998-12-31 | 2000-07-11 | Skw Biosystems | Production of fish gelatin |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006180744A (en) * | 2004-12-27 | 2006-07-13 | Yaizu Suisankagaku Industry Co Ltd | Method for cleaning fishskin |
| JP4694831B2 (en) * | 2004-12-27 | 2011-06-08 | 焼津水産化学工業株式会社 | How to clean fish skin |
| JP2007211053A (en) * | 2006-02-07 | 2007-08-23 | Miyagi Prefecture | Blue dye, blue collagen or gelatin and method for producing those |
| KR101100411B1 (en) | 2008-12-24 | 2011-12-30 | 대구가톨릭대학교산학협력단 | Curd Type Dombaeki Soup and Method for preparing the same |
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|---|---|
| JP3616046B2 (en) | 2005-02-02 |
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