JP2003063935A - Hair tonic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a novel hair tonic. SOLUTION: This hair tonic contains one or two or more extracts selected from the group of vegetable extracts consisting those of Mangifera such as mango, bindjai, kuweni mango, and Cucurbita such as Cucurbita moschata, Cucurbita maxima, Cucurbita pepo and zucchini.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a novel hair restorer.

[0002]

BACKGROUND OF THE INVENTION Mango (Mangifera indica) is an evergreen tree whose fruits and seeds are edible. Mango extracts are known to be used in cosmetics, bath agents and detergent compositions (Japanese Patent Laid-Open Nos. 2001-31552 and 2001-2001).
39823). Further, the mango seed extract includes lipstick compositions (JP-A-7-223924) and hair / scalp cosmetics having an effect of repairing damaged hair (JP-A-9-20843).
5), antioxidant compositions (JP-A-9-216838), bacteriostatic agents and antibacterial agents (JP-A-10-324610).

Pumpkin is an annual herbaceous plant and is an important vegetable for food. Pumpkins are known to be used for cosmetics (Japanese Patent Laid-Open No. 2000-290162). In addition, the squash seeds have a carcinogenic inhibitor (Japanese Patent Laid-Open No. 11-13998).
2), therapeutic agent for dysuria (Japanese Patent Laid-Open No. 2000-29019)
2), a method of using it for whitening cosmetics (JP-A-10-109924) is known.

However, a hair restorer containing a mango extract and a hair restorer containing a pumpkin extract are not known. Zucchini (Cucurbita pepo var. Melo
pepo) is similar in appearance to cucumber, but it is a variety of vegetable marlow, a family of pepo pumpkins. The pharmacological action of zucchini is not known, and the hair restorer containing the extract of zucchini is not known.

[0005]

An object of the present invention is to provide one or more extracts selected from the group consisting of extracts of plants belonging to the genus Mangifera and the genus Cucurbita as an active ingredient. It is to provide a hair restorer.

[0006]

Means for Solving the Problems The present invention includes the following (1) to
Regarding (5). (1) A hair restorer containing one or more kinds of extracts selected from the group consisting of extracts of plants belonging to the genus Mango and plants belonging to the genus Pumpkin. (2) The hair restorer according to (1), wherein the plant belonging to the genus Mango is mango, vinsey or odorous mango. (3) The hair restorer according to (1), wherein the plant belonging to the genus Pumpkin is zucchini, Japanese pumpkin, Atlantic squash, or Pepo squash. (4) The plant belonging to the genus Mango is mango (1)
The hair restorer described. (5) The hair restorer according to (1), wherein the plant belonging to the genus Pumpkin is zucchini.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION The hair restorer of the present invention contains one or more extracts selected from the group consisting of extracts of plants belonging to the genus Mangifera and the genus Cucurbita. . Examples of plants belonging to the genus Mango include mango, vinsey, odorous mango and the like. Examples of plants belonging to the genus Pumpkin include Japanese pumpkin, Atlantic squash, pepo squash, zucchini and the like.

The plant extract can be prepared by the following method. Fruits, seeds, leaves of the plant that is the raw material,
Stalks, roots, rhizomes and the like are collected at an appropriate time, and then used as they are or after being subjected to a drying process such as air drying, as a raw material for extraction. When the raw material is a liquid such as plant juice or sap, it can be used as it is as a raw material for extraction.

Extraction of the active ingredient from the above raw materials can be carried out by the following method. After the raw material is crushed or finely chopped, in the case of a liquid, the solvent is directly used for the batch or continuous extraction. As the extraction solvent,
Water or alcohols such as ethanol, methanol, isopropyl alcohol and the like, ketones such as acetone and methyl ethyl ketone, esters such as methyl acetate and ethyl acetate, and ethers such as diethyl ether and tetrahydrofuran. Hydrophilic or lipophilic solvents such as tellurium can be used alone or as a mixed solvent. The extraction can be usually performed at 0 to 100 ° C, preferably 5 to 50 ° C.

When the extraction is performed in a batch system, the extraction time is 1
The time is not less than about 10 days, and the amount of the solvent is usually 1 to 30 times, preferably 5 to 10 times the dry weight of the raw material. The extraction operation may be stirring or immersion. The extraction operation may be repeated a plurality of times as necessary. Examples of the continuous extraction method include a method using a Soxhlet extractor in which a reflux condenser and a siphon are combined, and the amount of solvent, extraction time, and the like are the same as the conditions of the batch extraction method described above.

As the extract of the present invention, the crude extract obtained by the above-mentioned procedure may be used as it is, or the insoluble residue may be further removed by filtration or centrifugation. Further, by purifying the active ingredients described below, water-soluble ionic substances such as salts, nonionic substances such as sugars and polysaccharides, oils, pigments, etc. are removed from the crude extract to improve hair growth activity. The extracted extract may be used.

The purification of the active ingredient from the crude extract can be carried out by any method as long as it is a method for separating and purifying a crude drug.
It is preferable to use a two-phase solvent partitioning method, a column chromatography method, a preparative high performance liquid chromatography method or the like alone or in combination. As the two-phase solvent distribution method, a method of extracting and removing oil-soluble components and pigments from the crude extract with n-hexane, petroleum ether, etc., and n-from the extract
Examples include a method of recovering the extract in the solvent phase by partitioning a solvent such as butanol or methyl ethyl ketone and water. Of these methods, the pH of the crude extract is adjusted to pH 2 to
7, preferably after adjusting the pH to 3 to 5 with hydrochloric acid or sodium hydroxide or the like, and then using a method of recovering the extract into the n-butanol phase by partitioning between n-butanol and water. .

As the column chromatography method, an ion exchange column chromatography method using Amberlite IR-120B (manufactured by Rohm and Haas Co.), Amberlite IRA-402 (manufactured by Rohm and Haas Co.) as a carrier, and a carrier as a carrier Adsorption column chromatography using styrene-divinylbenzene adsorbent resin such as phase silica gel, reverse phase silica gel, Diaion HP-20 (manufactured by Mitsubishi Chemical Co., Ltd.), Sepa beads SP-207 (manufactured by Mitsubishi Chemical Co., Ltd.), Sepha as a carrier Decks LH
A gel filtration method using -20 (manufactured by Pharmacia Biotech) and the like can be mentioned, and these can be used alone or in combination and can be used repeatedly.

Examples of the preparative high performance liquid chromatography method include a method using a reversed phase column using octadecyl silica and the like, a method using a normal phase column using silica gel / silica gel-NH _{2, and the} like. . The hair-growth agent may have any dosage form as long as it can contain the extract as an active ingredient. Used as a liquid or solid hair-growing agent by mixing with a suitable pharmaceutical base. Examples of liquid or solid hair-growing agents include liquid agents such as hair liquid, hair tonic, and hair lotion, and solid agents such as ointment and hair cream. The extract was added to each suitable base. Then, it can be produced by a conventional method. The amount of the extract compounded in the hair restorer of the present invention is usually 0.01 to 100% by weight, preferably 0.01 to 30% by weight, and particularly preferably 0.5 to 10% by weight.

Suitable bases for liquid formulations include those commonly used for hair restorers, such as purified water, ethyl alcohol, polyhydric alcohols, fats and oils, and additives may be added if necessary. Is also good. As polyhydric alcohol,
Glycerol, 1,3-butylene glycol, propylene glycol and the like can be mentioned. Examples of the oils and fats include wheat germ oil, camellia oil, jojoba oil, olive oil, squalane, safflower oil, macadamia nut oil, avocado oil, soybean hydrogenated lecithin and the like. Examples of the additives include fragrances, surfactants, bactericides and the like. Also, antioxidants,
UV absorbers, anti-inflammatory agents, cooling agents, moisturizers, vitamins,
A crude drug extract or the like may be added as appropriate.

As the fragrance, any fragrance may be used as long as it is usually used for cosmetics and the like. As a surfactant,
Polyoxyethylene (60) hydrogenated castor oil, polyoxyethylene (8) oleyl ether, polyoxyethylene monooleate (10), polyoxyethylene (30)
Glyceryl monostearate, sorbitan monostearate, polyoxyethylene (20) sorbitan monooleate, sucrose fatty acid ester, hexaglycerin monolaurate, polyoxyethylene reduced lanolin, polyoxyethylene (25) glyceryl pyroglutamate isostearic acid Examples thereof include diesters and N-acetylglutamine isostearyl esters.

As the bactericide, hinokitiol, triclosan, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol.
Examples include ru, azulene, salicylic acid, photosensitizer, zinc pyrithione and the like. Examples of the antioxidant include butylhydroxyanisole, gallic acid, propyl gallate, and erythorbic acid.

Examples of the ultraviolet absorber include benzophenones such as dihydroxybenzophenone, melanin, ethyl paraaminobenzoate, and paradimethylaminobenzoic acid.
-Ethylhexyl ester, sinoxate, para-methoxycinnamic acid 2-ethylhexyl ester, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, metal oxide fine particles and the like can be mentioned.

Examples of the anti-inflammatory agent include dipotassium glycyrrhizinate and allantoin. As a cooling agent,
Capsicum tincture, l-menthol and the like can be mentioned. Examples of the humectant include pyrrolidonecarboxylic acid, sodium hyaluronate, sodium chondroitin sulfate and the like.

As vitamins, dl-α-tocopherol acetate, dl-α-tocopherol acetate, vitamin E,
Examples thereof include benzyl nicotinate, D-pantothenyl alcohol, pantothenyl ethyl ether, biotin, pyridoxine hydrochloride, riboflavin and the like. Examples of the crude drug extract include senburi extract, garlic extract, carrot extract, aloe extract and the like.

When the above liquid dosage form is used as a spray, it is preferable to use a non-combustible liquefied gas. Examples of solid dosage form bases include petrolatum, solid paraffin, vegetable oils, mineral oils, lanolin, waxes, macrogol, and the like, if necessary, the above additives, emulsifiers such as lecithin,
Lower alcohols such as ethanol and isopropyl alcohol may be added.

The dose of the hair restorer of the present invention is
1 to 600m as an extract per adult, depending on symptoms, therapeutic effect, administration method, treatment time, etc.
g, preferably 10 to 300 mg, is administered transdermally once to several times a day. Hereinafter, examples and test examples of the present invention will be shown.

[0023]

Example 1 A method for preparing a hair-growth tonic containing a mango extract will be described below. (1) Manufacture of Mango Extracted Fraction 3.5 kg of mango fruit (including seeds) was sufficiently crushed with a food processor PS-3000S (manufactured by Chubu Koki Co., Ltd.), and then 7 L of 70 (W / W)% acetone. Aqueous solution [(W / W)% represents the weight percentage with respect to the mixed solution with water. ], And ultrasonic cleaner WT-1200-40
(Honda Electronics Co., Ltd.) ultrasonic treatment was performed and immersion extraction was performed at room temperature for 1 hour. It was filtered using miraculose (Biochem) and separated into a filtrate and a residue. Acetone to the residue 7
L was added, and immersion extraction was performed at room temperature for 1 hour while performing ultrasonic treatment. A filtrate was obtained in the same manner as above and mixed with the previously obtained filtrate. This mixed filtrate was rotary evaporated.
450 ml after removing acetone by vacuum concentration
It was passed through a column packed with DIAION HP-20 resin (manufactured by Mitsubishi Chemical Co., Ltd.) and adsorbed. Methanol 900m
The column was washed with 1 and then eluted with 900 ml of acetone.
The acetone eluate was dried under reduced pressure to obtain 3 g of mango extract. (2) Preparation of hair-growth tonic containing mango extract 6.4 kg of ethyl alcohol, 2 kg of glycerol, 1.6 kg of purified water and 100 g (1% by weight) or 300 g (3%) of the mango extract obtained in the above (1). (% By weight) was uniformly mixed with stirring to dissolve the solid matter to prepare a hair-growth tonic. All the above operations were performed at room temperature. Example 2 A method for preparing a hair-growth tonic containing a zucchini extract is described below. (1) Manufacture of zucchini extract 1.6 kg of zucchini was sufficiently crushed with a food processor, and then 7 L of 50 (V / V)% methanol aqueous solution [(V / V)% was used as a volume percentage relative to a mixed solution with water. Represent The following description is also the same. ] Was added, and immersion extraction was performed at room temperature for 1 hour while performing ultrasonic treatment. It was filtered using miraculose and separated into a filtrate and a residue. The residue was again subjected to immersion extraction using 7 L of 50 (V / V)% methanol aqueous solution. A filtrate was obtained in the same manner as above and mixed with the previously obtained filtrate. This mixed filtrate was rotary evaporated.
450 ml after removing acetone by vacuum concentration
The column was passed through a column packed with Diaion HP-20 resin, adsorbed, and eluted with 900 ml of methanol. The methanol eluate was dried under reduced pressure to obtain 18 g of zucchini extract. (2) Preparation of hair-growth tonic containing zucchini extract By the same method as in Example 1 (2) except that the mango extract obtained in Example 1 was replaced with a zucchini extract.
A hair-growth tonic containing a zucchini extract was prepared. Example 3 250 ml of a hair-growth tonic containing the 3% mango extract prepared in Example 1 and 250 ml of a hair-growth tonic containing the 3% zucchini extract prepared in Example 2 were mixed to obtain a 1.5% mango extract. And 500 ml of a hair growth tonic containing 1.5% zucchini extract was prepared. Test Example 1 Confirmation Test of Hair Growth Effect in Mice With reference to the method of Ogawa et al. (The Journal of Deumatology, vol. 10, p. 45-54, 1983), the test of hair growth effect in mice is performed as follows. I went.

8-week-old C3H / H in the resting period of the hair cycle
The back hair of an eNCrj male mouse (purchased from Charles River Japan, Inc.) was shaved with an electric clipper and an electric shaver, and the hair-growth tonics prepared in Examples 1 and 2 were applied once a day to 150 μl of each hair-shaping portion. Applied. A tonic base (64% ethanol, 20% propylene glycol, 16% purified water) was applied to the control group (five animals in each group). After taking a photograph of the back of the mouse 17 days after the start of application, image processing software (NIH Image 1.
61 / ppc) was used to determine the area ratio of the hair-growth part to the hair-removal part, and the hair-growth effect was determined.

The results are shown in Table 1. In the extract-applied group, no findings showing abnormalities such as inflammation were observed in the applied part. As a result of the test, the 1% and 3% mango extracts were found to have an effect of improving the average hair growth area of 10% or more compared with the control. The 1% zucchini extract fraction was found to have an average hair growth area improving effect of about 10% with respect to the control. The 3% zucchini extract fraction was found to have an effect of improving the average hair growth area of 20% with respect to the control.

[0026]

[Table 1]

[0027]

Industrial Applicability According to the present invention, it is possible to provide a hair restorer containing one or more extracts selected from the group consisting of extracts of plants belonging to the genera Mango and Pumpkin.

   ─────────────────────────────────────────────────── ─── Continued front page    F-term (reference) 4C083 AA111 AA112 AC102 AC122                       CC37 EE22                 4C088 AB19 AB21 AC04 BA08 NA14                       ZA92

Claims (5)

[Claims] 1. A hair restorer containing one or more extracts selected from the group consisting of extracts of plants belonging to the genus Mango and plants belonging to the genus Pumpkin. 2. The hair restorer according to claim 1, wherein the plant belonging to the genus Mango is mango, vinsey or odorous mango. 3. The hair restorer according to claim 1, wherein the plant belonging to the genus Pumpkin is zucchini, Japanese squash, Atlantic squash or Pepo squash. 4. The hair restorer according to claim 1, wherein the plant belonging to the genus Mango is mango. 5. The hair restorer according to claim 1, wherein the plant belonging to the genus Pumpkin is zucchini.