JP2003014766A - Biochemistry analyzing kit containing biochemistry analyzing unit and storage phosphor sheet, and exposure device of the storage phosphor sheet - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a biochemistry analyzing kit containing a biochemistry analyzing unit and a storage phosphor sheet in which examination precision of developing abnormalities of a factor can be raised when a substance derived from an organism labeled by a radioactive labeled substance is coupled to a particularly coupled substance included in a spot-like area formed in the biochemistry analyzing unit by hybridization or the like, and the factor is subjected to developing examination by use of the biochemistry analyzing unit and the storage phosphor sheet formed with a stimulable phosphor layer. SOLUTION: A biochemistry analyzing kit contains a biochemistry analyzing unit provided with an adsorption layer 4 formed with an adsorptive material and the storage phosphor sheet formed with the stimulable phosphor layer, and at least each part of the biochemistry analyzing unit and the storage phosphor sheet has a complementary shape to each other.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a biochemical analysis kit including a biochemical analysis unit and a stimulable phosphor sheet, and an exposure apparatus for the stimulable phosphor sheet. Specific substances contained in a spot-like region formed in the biochemical analysis unit were selectively collected by radiolabeling from a person who had abnormal expression and were labeled with a radiolabeled substance. Bind specifically to the binding substance,
The biochemical analysis unit and the stimulable phosphor layer-formed stimulable phosphor sheet are superposed and exposed with a radioactive labeling substance selectively contained in the spot-shaped region, and then exposed to a radioactive label. The photostimulable phosphor layer exposed by the substance is scanned with excitation light to excite the photostimulable phosphor, and the photostimulable light emitted from the photostimulable phosphor is photoelectrically detected to obtain biochemistry. In addition to generating analysis data, biologically-derived substances collected from healthy subjects and labeled with radioactive labeling substances are selectively included in the spot-like region formed in the biochemical analysis unit by hybridization, etc. Specifically bound to the specific binding substance
The biochemical analysis unit and the stimulable phosphor layer-formed stimulable phosphor sheet are superposed and exposed with a radioactive labeling substance selectively contained in the spot-shaped region, and then exposed to a radioactive label. The photostimulable phosphor layer exposed by the substance is scanned with excitation light to excite the photostimulable phosphor, and the photostimulable light emitted from the photostimulable phosphor is photoelectrically detected to obtain biochemistry. A biochemical analysis unit and a stimulable phosphor sheet that can improve the test accuracy of gene expression abnormality when performing analysis by generating analysis data and comparing the obtained biochemical analysis data The present invention relates to a biochemical analysis kit including: and an exposure apparatus for a stimulable phosphor sheet.

[0002]

2. Description of the Related Art When a radiation is irradiated, the energy of the radiation is absorbed, stored and recorded, and then excited by using an electromagnetic wave of a specific wavelength range. A photostimulable phosphor having the property of emitting a stimulating amount of light is used as a radiation detection material, and a substance having a radioactive label is administered to an organism, and then the organism or tissue of the organism is treated. A portion of the sample is used as a sample, and this sample is overlapped with a stimulable phosphor sheet provided with a stimulable phosphor layer for a certain period of time to store and record radiation energy in the stimulable phosphor. , Scanning the stimulable phosphor layer with electromagnetic waves to excite the stimulable phosphor and photoelectrically detecting the stimulable light emitted from the stimulable phosphor to generate a digital image signal. Image processing, CRT On a recording material such as a display unit or on the photographic film, the autoradiographic analyzing system is configured to reproduce an image has been known (for example, Kokoku 1-70884 and JP Kokoku 1-70
882, Japanese Patent Publication No. 4-3962, etc.).

An autoradiography analysis system using a stimulable phosphor sheet as a radiation detecting material not only requires a chemical treatment called a developing treatment, unlike the case where a photographic film is used, but also was obtained. By subjecting the digital data to data processing, there is an advantage that the analysis data can be reproduced or a quantitative analysis by a computer can be performed as desired.

On the other hand, there is known a fluorescence analysis system using a fluorescent substance such as a fluorescent dye as a labeling substance instead of the radioactive labeling substance in the autoradiography analysis system. According to this fluorescence analysis system, by detecting the fluorescence emitted from the fluorescent substance, the gene sequence, the expression level of the gene, the metabolism, absorption, and excretion routes of the administered substance in the experimental mouse, the state, the separation of the protein, Identification or evaluation of molecular weight and characteristics can be performed. For example, a solution containing plural kinds of protein molecules to be electrophoresed is electrophoresed on the gel support, and then the gel support is subjected to fluorescence. An image is generated by staining the electrophoresed protein by immersing it in a solution containing a dye, exciting the fluorescent dye with excitation light, and detecting the resulting fluorescence, and then producing an image on the gel support. The position and quantitative distribution of protein molecules can be detected. Alternatively, by Western blotting,
A probe and a protein molecule prepared by transferring at least a part of the electrophoresed protein molecule onto a transfer support such as nitrocellulose and labeling an antibody that specifically reacts with the target protein with a fluorescent dye are prepared. By selectively associating and selectively labeling a protein molecule that binds only to an antibody that specifically reacts,
By exciting the fluorescent dye with the excitation light and detecting the generated fluorescence, an image can be generated and the position and quantitative distribution of the protein molecule on the transfer support can be detected. In addition, after adding a fluorescent dye to a solution containing a plurality of DNA fragments to be electrophoresed, the plurality of DNA fragments are electrophoresed on a gel support, or on a gel support containing a fluorescent dye. , A plurality of DNA fragments are electrophoresed, or a plurality of DNA fragments are electrophoresed on a gel support, and then the gel support is immersed in a solution containing a fluorescent dye. DNA fragments are labeled, a fluorescent dye is excited by excitation light, and the resulting fluorescence is detected to generate an image, and the distribution of DNA on the gel support is detected, or a plurality of DNAs are detected.
The fragments are electrophoresed on a gel support, followed by DNA
Denaturation, and then by Southern blotting, at least a part of the denatured DNA fragment is transferred onto a transfer support such as nitrocellulose, and DNA or RNA complementary to the target DNA is fluorescent dye. A probe DNA or probe RN prepared by hybridizing a probe prepared by labeling with
Only the DNA fragment complementary to A is selectively labeled, the fluorescent dye is excited by the excitation light, and the resulting fluorescence is detected to generate an image, so that the DNA of interest on the transfer support is detected. The distribution can be detected. further,
DN containing a target gene labeled with a labeling substance
A DNA probe complementary to A is prepared, hybridized with the DNA on the transcription support, and the enzyme is allowed to bind to the complementary DNA labeled with a labeling substance, and then contacted with a fluorescent substrate for fluorescence. An image is generated by changing the substrate to a fluorescent substance that emits fluorescence, exciting the generated fluorescent substance with excitation light, and detecting the generated fluorescence,
It is also possible to detect the distribution of the target DNA on the transcription support. This fluorescence analysis system has an advantage that gene sequences and the like can be easily detected without using radioactive substances.

Similarly, a substance derived from a living body such as a protein or a nucleic acid is immobilized on a support and is selectively labeled with a labeling substance which causes chemiluminescence by contacting with a chemiluminescent substrate. A selectively labeled biological substance is brought into contact with a chemiluminescent substrate, and chemiluminescence in the visible light wavelength region generated by the contact between the chemiluminescent substrate and the labeled substance is photoelectrically detected to obtain a digital image signal. Chemiluminescence for generating information, reproducing the chemiluminescence image on a display material such as a CRT or a recording material such as a photographic film by performing image processing, and obtaining information on a substance of biological origin such as gene information. Analysis systems are also known.

Further, in recent years, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, etc. have been found at different positions on the surface of a carrier such as a slide glass plate and a membrane filter.
Other proteins, nucleic acids, cDNA, DNA, RNA
Such as specific binding substances that can specifically bind to a substance of biological origin and whose base sequence, base length, composition, etc. are known, are dropped using a spotter device, and a large number of independent Then, a spot is formed, and then hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNAs, DNAs, mRNAs, etc. are collected from the living body by extraction, isolation, etc., or, Substances of biological origin that have been subjected to chemical treatment, chemical modification, etc., labeled with a labeling substance such as a fluorescent substance or a dye, can be specifically bound to a specific binding substance by hybridization. The bound microarray is irradiated with excitation light, and light such as fluorescence emitted from a labeling substance such as a fluorescent substance or dye is photoelectrically detected to obtain a substance derived from a living body. Microarray analysis system that analyzes have been developed. According to this microarray analysis system, a large number of spots of specific binding substances are formed at high density at different positions on the surface of a carrier such as a slide glass plate or a membrane filter, and a substance of biological origin labeled with a labeling substance is used. By hybridizing with, there is an advantage that a substance derived from a living body can be analyzed in a short time.

[0007] Further, hormones, tumor markers, enzymes,
Antibodies, antigens, abzymes, other proteins, nucleic acids,
A specific binding substance, such as cDNA, DNA, or RNA, which can be specifically bound to a substance of biological origin and whose base sequence, base length, composition, etc. is known, is dropped using a spotter device. , Multiple independent spots, then hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNA, DN
Substances derived from a living body, such as A and mRNA, which have been collected from the living body by extraction, isolation, etc., or which have been further subjected to chemical treatment, chemical modification, etc., and which have been labeled with radiolabeled substances , By hybridization or the like, to the specific binding substance, the macroarray specifically bound, is brought into close contact with the stimulable phosphor sheet on which the stimulable phosphor layer containing the stimulable phosphor is formed, The photostimulable phosphor layer is exposed to light, and then the photostimulable phosphor layer is irradiated with excitation light, and the photostimulable light emitted from the photostimulable phosphor layer is photoelectrically detected for biochemical analysis. A macroarray analysis system using a radiolabeled substance that generates use data and analyzes a substance derived from a living body has also been developed.

[0008]

In a macroarray analysis system using a radiolabeled substance, a specific binding substance is dropped onto a biochemical analysis unit such as a membrane filter to form a large number of spot-shaped regions. Formed and collected from a person with abnormal expression of the gene, a substance of biological origin labeled with a radioactive labeling substance, by hybridization etc., is selectively bound to the specific binding substance contained in the spot-like region, By specifically binding, a biochemical analysis unit such as a membrane filter and a stimulable phosphor layer-formed storage phosphor sheet are overlaid,
With a radioactive labeling substance selectively contained in the spot-like region, after exposure, the stimulable phosphor layer exposed by the radioactive labeling substance is scanned with excitation light to excite the stimulable phosphor. Photostimulated photostimulable light emitted from the photostimulable phosphor was detected photoelectrically to generate data for biochemical analysis, while spot-like regions of the same specific binding substance were formed in the same pattern. The same specific binding substance is dropped on a biochemical analysis unit such as a membrane filter to form spot-like regions having the same pattern, collected from a healthy person, and the substance of biological origin labeled with a radiolabel substance is labeled. Selectively binds to the specific binding substance contained in the spot-shaped region by hybridization, etc., and the unit for biochemical analysis such as a membrane filter is used. Of a stimulable fluorescent substance, which is exposed by a radiolabeling substance after being exposed by a radiolabeling substance selectively contained in a spot-shaped region by superimposing a stimulable phosphor sheet on which a luminescent phosphor layer is formed. The body layer is scanned with excitation light to excite the stimulable phosphor, and photoelectrically detect the stimulable light emitted from the stimulable phosphor to generate data for biochemical analysis. By comparison, a method of conducting an inspection is generally adopted.

However, it is generally very difficult to drop the same specific binding substance on a biochemical analysis unit such as a membrane filter to form spot-like regions having the same pattern, and it is also difficult to accumulate the fluorescent light. Since it is generally difficult to uniformly form a stimulable phosphor layer on the body sheet, it is collected from a person whose expression abnormality is recognized in the gene, and a radioactive substance is used to label the substance of biological origin, Selectively obtained by exposing the stimulable phosphor layer of the stimulable phosphor sheet to a specific binding substance, and the radioactive labeling substance selectively contained in the spot-shaped region. Data for biochemical analysis and radio-labeled substances collected from healthy individuals and labeled with a substance of biological origin are selectively bound to a specific binding substance, and spotted. The accuracy of the test is low when the stimulable phosphor layer of the stimulable phosphor sheet is exposed by the radioactive labeling substance selectively contained in the region and the obtained biochemical analysis data is compared. There was a problem.

Therefore, according to the present invention, a substance derived from a living body, which is collected from a person whose gene is abnormally expressed and is labeled with a radiolabeled substance, is selectively formed into a biochemical analysis unit by hybridization or the like. Specific binding substance contained in the spot-shaped area, specifically bound, the biochemical analysis unit, and the stimulable phosphor layer is formed by superimposing the stimulable phosphor sheet formed,
With a radioactive labeling substance selectively contained in the spot-like region, after exposure, the stimulable phosphor layer exposed by the radioactive labeling substance is scanned with excitation light to excite the stimulable phosphor. Photostimulated photostimulable light emitted from the photostimulable phosphor is photoelectrically generated to generate data for biochemical analysis, and at the same time, a substance of biological origin collected from a healthy person and labeled with a radiolabel substance is By means of hybridization, etc., the biochemical analysis unit and the stimulable phosphor layer are selectively bound to the specific binding substance contained in the spot-like region formed in the biochemical analysis unit. A stimulable phosphor layer, which is formed by stacking the formed stimulable phosphor sheet and exposing by the radioactive labeling substance selectively contained in the spot-shaped region, and then exposed by the radioactive labeling substance. Scanning with excitation light to excite the stimulable phosphor, photoelectrically detecting the stimulable light emitted from the stimulable phosphor to generate biochemical analysis data, and obtain the obtained biochemistry. A biochemical analysis kit including a biochemical analysis unit and a stimulable phosphor sheet and a stimulable phosphor that can improve the inspection accuracy of gene expression abnormalities by comparing analysis data and performing a test It is an object of the present invention to provide a sheet exposure apparatus.

[0011]

The object of the present invention is to:
A biochemical analysis unit having an adsorption layer formed of an adsorptive material, and a stimulable phosphor sheet having a stimulable phosphor layer formed thereon, wherein the biochemical analysis unit and the stimulable phosphor sheet are included. Are each at least partly complementary in shape to each other.

According to the present invention, the specific binding substance is dropped in a spot shape onto the adsorption layer of the biochemical analysis unit constituting the biochemical analysis kit to form a plurality of spot-shaped areas separated from each other. , Specific binding contained in multiple spot-like regions selectively by hybridization etc. from biologically-derived substances that were collected from humans or healthy individuals with abnormal gene expression and labeled with radiolabeled substances By specifically binding to a substance and selectively labeling the spot-like region with a radioactive labeling substance, a stimulable phosphor layer is formed, and it is overlaid on a stimulable phosphor sheet that constitutes the same biochemical analysis kit. In addition, by the radioactive labeling substance selectively contained in the plurality of spot-shaped regions,
Exposing, scanning the exposed photostimulable phosphor layer with excitation light, exciting the photostimulable phosphor, photoelectrically detecting the photostimulable light emitted from the photostimulable phosphor, After generating data for chemical analysis, remove biogenic substances from multiple spot-like regions of the biochemical analysis unit by rehybridization, etc., and collect from healthy individuals or people with abnormal gene expression. , A biologically-derived substance labeled with a radiolabeling substance is selectively bound again to a specific binding substance contained in a plurality of spot-like regions by hybridization, etc. , The spot-shaped region is selectively labeled, and the stimulable phosphor layer formed on the stimulable phosphor sheet is exposed by a radioactive labeling substance contained in the spot-shaped region to obtain a healthy image. When generating data for biochemical analysis of humans or people with abnormal expression of genes, the unit for biochemical analysis and the data for biochemical analysis of people with abnormal expression of genes or healthy people were generated. The stimulable phosphor sheet used at this time constitutes one biochemical analysis kit, and at least a part of each has a shape complementary to each other, and therefore is the same as the biochemical analysis unit. Use the biochemical analysis kit to create biochemical analysis data for a person with normal gene expression or healthy individuals, and use the biochemical analysis unit to erase accumulated radiation energy. The stored stimulable phosphor sheet can be reliably selected and exposed using the biochemical analysis unit. Even if the stimulable phosphor layer cannot be formed uniformly on the stimulable phosphor sheet, it is not possible to form a spot-shaped region having the same pattern by dropping a selective binding substance. It is possible to significantly improve the inspection accuracy of abnormal expression.

[0013] In a preferred aspect of the present invention, the biochemical analysis unit and the stimulable phosphor sheet are configured to form a plurality of through holes, and the biochemical analysis unit and the stimulable phosphor are formed. At least one through hole is formed in each of the sheets so as to have a shape complementary to each other.

In a further preferred aspect of the present invention, at least one substantially circular through hole is formed in each of the biochemical analysis unit and the stimulable phosphor sheet so as to have complementary shapes. Has been done.

In another preferred aspect of the present invention, the biochemical analysis unit and the stimulable phosphor sheet are configured to form a plurality of cutouts, and the biochemical analysis unit and the stimulable phosphor sheet are formed. At least one notch is formed in each of the phosphor sheets so as to have a shape complementary to each other.

[0016] In a further preferred aspect of the present invention, the biochemical analysis unit and the stimulable phosphor sheet each have at least one substantially semicircular cutout so as to have complementary shapes to each other. Has been formed.

[0017] In a preferred aspect of the present invention, a specific binding substance having a known structure or characteristic is dropped on the adsorption layer of the biochemical analysis unit to form a plurality of spot-shaped regions separated from each other. ing.

[0018] In a preferred aspect of the present invention, the biochemical analysis unit includes a substrate having a plurality of holes formed therein, and the adsorption layer is constituted by an absorptive region formed in the plurality of holes. Has been done.

[0019] In a preferred aspect of the present invention, the absorptive region of the biochemical analysis unit is formed by filling an absorptive material into the plurality of holes of the substrate.

[0020] In a further preferred aspect of the present invention, the absorptive region of the biochemical analysis unit is formed by press-fitting an absorptive film containing an absorptive material into the plurality of holes of the substrate. Has been done.

In a preferred aspect of the present invention, the absorptive region of the biochemical analysis unit is regularly formed on the substrate.

[0022] In a preferred aspect of the present invention, the absorptive region of the biochemical analysis unit is formed into a substantially circular shape.

[0023] In a preferred aspect of the present invention, the absorptive region of the biochemical analysis unit is formed into a substantially rectangular shape.

[0024] In a preferred aspect of the present invention, 10 or more absorptive regions are formed on the substrate of the biochemical analysis unit.

In a further preferred aspect of the present invention, the substrate of the biochemical analysis unit is formed with 100 or more absorptive regions.

[0026] In a further preferred aspect of the present invention, the substrate of the biochemical analysis unit is provided with 1000
The absorptive region described above is formed.

[0027] In a further preferred aspect of the present invention, the substrate of the biochemical analysis unit is provided with 1000
Zero or more of the absorptive regions are formed.

[0028] In a further preferred aspect of the present invention, the substrate of the biochemical analysis unit is provided with 1000
00 or more of the absorptive regions are formed.

[0029] In a preferred embodiment of the present invention, the adsorptive region has a size of less than 5 mm 2.

[0030] In a further preferred aspect of the present invention, the absorptive region has a size of less than 1 mm 2.

[0031] In a further preferred aspect of the present invention, the absorptive region has a size of less than 0.5 mm 2.

[0032] In a further preferred aspect of the present invention, the absorptive region has a size of less than 0.1 mm 2.

[0033] In a further preferred aspect of the present invention, the absorptive region has a size of less than 0.05 mm 2.

In a further preferred aspect of the present invention, the absorptive region has a size of less than 0.01 mm 2.

[0035] In a preferred aspect of the present invention, the absorptive regions are formed on the substrate of the biochemical analysis unit at a density of 10 or more per cm 2.

[0036] In a further preferred aspect of the present invention, the absorptive regions are formed in the substrate of the biochemical analysis unit at a density of 50 or more per cm 2.

In a further preferred aspect of the present invention, the absorptive regions are formed on the substrate of the biochemical analysis unit at a density of 100 or more per cm 2.

In a further preferred aspect of the present invention, the absorptive regions are formed on the substrate of the biochemical analysis unit at a density of 500 or more per cm 2.

[0039] In a further preferred aspect of the present invention, the absorptive regions are formed in the substrate of the biochemical analysis unit at a density of 1,000 or more per cm 2.

[0040] In a further preferred aspect of the present invention, the absorptive regions are formed in the substrate of the biochemical analysis unit at a density of 5,000 or more per cm 2.

[0041] In a further preferred aspect of the present invention, the absorptive regions are formed in the substrate of the biochemical analysis unit at a density of 10,000 or more per cm 2.

[0042] In a preferred aspect of the present invention, a specific binding substance having a known structure or characteristic is dropped onto the adsorptive region of the biochemical analysis unit.

In the present invention, a porous material or a fibrous material is preferably used as the adsorptive material forming the adsorptive layer or the adsorptive region of the biochemical analysis unit. The porous material and the fiber material may be used in combination to form the adsorption layer or the adsorption region.

In the present invention, the porous material used to form the adsorption layer or the adsorption region of the biochemical analysis unit may be either an organic material or an inorganic material,
It may be an organic / inorganic composite.

In the present invention, the organic porous material used for forming the adsorption layer or the adsorption region of the biochemical analysis unit is not particularly limited,
A carbon porous material such as activated carbon or a porous material capable of forming a membrane filter is preferably used. Specifically, nylon 6, nylon 6,6, nylon 4,
10, such as nylons, cellulose derivatives such as nitrocellulose, cellulose acetate, cellulose acetate butyrate, collagen, alginic acid, calcium alginate, alginic acid such as alginic acid / polylysine polyion complex, polyolefins such as polyethylene and polypropylene, polyvinyl chloride, poly Examples thereof include polyfluoride such as vinylidene chloride, polyvinylidene fluoride, and polytetrafluoride, and copolymers or composites thereof.

In the present invention, the inorganic porous material used for forming the adsorption layer or the adsorption region of the biochemical analysis unit is not particularly limited,
Examples thereof include metals such as platinum, gold, iron, silver, nickel and aluminum, metal oxides such as alumina, silica, titania and zeolite, metal salts such as hydroxyapatite and calcium sulfate, and complexes thereof.

In the present invention, the fibrous material used for forming the adsorption layer or the adsorption region of the biochemical analysis unit is not particularly limited, but for example, nylon 6, nylon 6,6, Nylons such as nylon 4,10, nitrocellulose, cellulose acetate,
Examples thereof include cellulose derivatives such as cellulose butyrate acetate.

In the present invention, the adsorptive region of the biochemical analysis unit is subjected to electrolytic treatment, plasma treatment, oxidation treatment such as arc discharge, primer treatment using silane coupling agent, titanium coupling agent, etc., surfactant. It can also be formed by surface treatment such as treatment.

In a preferred aspect of the present invention, the substrate of the biochemical analysis unit has a property of attenuating radiation.

According to a preferred embodiment of the present invention, since the substrate of the biochemical analysis unit has a property of attenuating radiation, the stimulable phosphor layer of the stimulable phosphor sheet is adsorbed. When exposed by the radioactive labeling substance contained in the area, the electron beam (β-ray) emitted from the radioactive labeling substance contained in each absorptive area is scattered in the substrate and emitted from the adjacent absorptive area. It is possible to reliably prevent the scattered electron beam (β-ray) from entering the region of the stimulable phosphor layer to be exposed by the radioactive labeling substance thus exposed by the radioactive labeling substance. The photostimulable phosphor layer is irradiated with excitation light, and the photostimulable light emitted from the photostimulable phosphor layer is photoelectrically detected to generate data for biochemical analysis, and a substance derived from a living body is detected. Even when analysis is performed, It is possible to effectively prevent the generation of noise in the biochemical analysis data due to the scattering of the electron beam (β-ray).

In a preferred aspect of the present invention, when the substrate of the biochemical analysis unit is exposed to radiation by a distance equal to the distance between the adjacent absorptive regions, the radiation is Energy of 1/5
It has the following property of damping.

In a further preferred aspect of the present invention, when the substrate of the biochemical analysis unit transmits radiation through the substrate by a distance equal to the distance between the adsorbing regions adjacent to each other, The energy of radiation
It has the property of being attenuated to 1/10 or less.

In a further preferred aspect of the present invention, when the substrate of the biochemical analysis unit has radiation transmitted through the substrate by a distance equal to the distance between the adjacent absorptive regions, The energy of radiation
It has the property of being attenuated to 1/50 or less.

In a further preferred aspect of the present invention, when the substrate of the biochemical analysis unit has radiation transmitted through the substrate by a distance equal to the distance between the adjacent absorptive regions, The energy of radiation
It has the property of being attenuated to 1/100 or less.

In a further preferred aspect of the present invention, when the substrate of the biochemical analysis unit has radiation transmitted through the substrate by a distance equal to the distance between the adsorbing regions adjacent to each other, The energy of radiation
It has the property of being attenuated to 1/500 or less.

In a further preferred aspect of the present invention, when the substrate of the biochemical analysis unit has radiation transmitted through the substrate by a distance equal to the distance between the adjacent absorptive regions, The energy of radiation
It has the property of being attenuated to 1/1000 or less.

In the present invention, the material for forming the substrate of the biochemical analysis unit is preferably one having a property of attenuating radiation, and the material having a property of attenuating radiation is not particularly limited. But not
Both inorganic compound materials and organic compound materials can be used, and metal materials, ceramic materials or plastic materials are particularly preferably used.

In the present invention, as the inorganic compound material which can be preferably used for forming the substrate of the biochemical analysis unit, for example, gold, silver, copper, zinc,
Metals such as aluminum, titanium, tantalum, chromium, iron, nickel, cobalt, lead, tin and selenium; alloys such as brass, stainless steel and bronze; silicon materials such as silicon, amorphous silicon, glass, quartz, silicon carbide and silicon nitride. Metal oxides such as aluminum oxide, magnesium oxide and zirconium oxide; inorganic salts such as tungsten carbide, calcium carbonate, calcium sulfate, hydroxyapatite and gallium arsenide. These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic.

In the present invention, a polymer compound is preferably used as the organic compound material which can be preferably used for forming the substrate of the biochemical analysis unit, and the polymer compound preferably used is, for example, Polyolefins such as polyethylene and polypropylene; polymethylmethacrylate, butylacrylate /
Acrylic resins such as methylmethacrylate copolymers; polyacrylonitrile; polyvinyl chloride; polyvinylidene chloride; polyvinylidene fluoride; polytetrafluoroethylene; polychlorotrifluoroethylene; polycarbonate; polyesters such as polyethylene naphthalate and polyethylene terephthalate; nylon 6 Polyamide; Polysulfone; Polyphenylene sulfide; Silicon resin such as polydiphenylsiloxane; Phenolic resin such as novolac; Epoxy resin; Polyurethane; Polystyrene; Butadiene-styrene copolymer; Cellulose Polysaccharides such as cellulose acetate, nitrocellulose, starch, calcium alginate, hydroxypropylmethylcellulose; ; Chitosan; lacquer; gelatin, collagen, copolymers of polyamides and these high molecular compounds, such as keratin, and the like.
These may be a composite material, if necessary, can be filled with metal oxide particles or glass fiber,
It is also possible to blend and use an organic compound material.

Generally, the higher the specific gravity is, the higher the radiation attenuating ability is. Therefore, the substrate of the biochemical analysis unit is preferably formed of a compound material or a composite material having a specific gravity of 1.0 g / cm ³ or more, Specific gravity is 1.5g / c
It is particularly preferable that it is formed of a compound material or a composite material of m ³ or more and 23 g / cm ³ or less.

In a preferred embodiment of the present invention, the stimulable phosphor sheet comprises a support having a plurality of holes formed therein, and the stimulable phosphor layer is formed in the plurality of holes. The photostimulable phosphor layer region is formed according to the same pattern as the absorptive region of the biochemical analysis unit.

In a preferred embodiment of the present invention, the stimulable phosphor layer region of the stimulable phosphor sheet is filled with stimulable phosphor in the plurality of holes of the support, Has been formed.

[0063] In a further preferred aspect of the present invention, the stimulable phosphor layer region of the stimulable phosphor sheet comprises a stimulable phosphor in the plurality of holes of the support. Fluorescent phosphor film is formed by being press-fitted.

[0064] In a preferred aspect of the present invention, the stimulable phosphor sheet includes a support, and the stimulable phosphor layer is provided on the surface of the support, the adsorptive property of the biochemical analysis unit. It is composed of a plurality of photostimulable phosphor layer regions formed according to the same pattern as the regions.

In a preferred embodiment of the present invention, the stimulable phosphor layer region of the stimulable phosphor sheet is 5
It has a size of less than a square millimeter.

In a further preferred aspect of the present invention, the stimulable phosphor layer region of the stimulable phosphor sheet has a size of less than 1 mm 2.

In a further preferred aspect of the present invention, the stimulable phosphor layer region of the stimulable phosphor sheet has a size of less than 0.5 mm 2.

In a further preferred aspect of the present invention, the stimulable phosphor layer region of the stimulable phosphor sheet has a size of less than 0.1 mm 2.

[0069] In a further preferred aspect of the present invention, the stimulable phosphor layer region of the stimulable phosphor sheet has a size of less than 0.05 mm 2.

[0070] In a further preferred aspect of the present invention, the stimulable phosphor layer region of the stimulable phosphor sheet has a size of less than 0.01 mm 2.

In a preferred embodiment of the present invention, the support of the stimulable phosphor sheet has a property of attenuating radiation.

According to a preferred embodiment of the present invention, since the support of the stimulable phosphor sheet has a property of attenuating radiation, the stimulable phosphor layer of the stimulable phosphor sheet is adsorbed. When exposed by the radioactive labeling substance contained in the absorptive region, the electron beam (β-ray) emitted from the radioactive labeling substance contained in each absorptive region scatters inside the support of the stimulable phosphor sheet. , It is possible to reliably prevent the scattered electron beam (β-ray) from entering the region of the stimulable phosphor layer to be exposed by the radioactive labeling substance emitted from the adjacent adsorptive region, Therefore, by irradiating the photostimulable phosphor layer exposed by the radiolabeled substance with excitation light, photoelectrically detecting the photostimulable light emitted from the photostimulable phosphor layer to obtain data for biochemical analysis. When generating and analyzing substances of biological origin Also, it becomes possible to effectively prevent the noise due to the scattering of the electron beam (β-ray) emitted from the radiolabeled substance from being generated in the biochemical analysis data.

[0073] In a preferred aspect of the present invention, the support of the stimulable phosphor sheet is so arranged that the radiation in the support is equal to the distance between the adjacent photostimulable phosphor layer regions. When transmitted, it has the property of attenuating the energy of radiation to ⅕ or less.

In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet is separated by a distance equal to the distance between adjacent photostimulable phosphor layer regions.
It has the property of attenuating the energy of the radiation to 1/10 or less when the radiation passes through the support.

In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet is separated by a distance equal to the distance between adjacent photostimulable phosphor layer regions.
It has the property of attenuating the energy of the radiation to 1/50 or less when the radiation passes through the support.

In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet is separated by a distance equal to the distance between adjacent photostimulable phosphor layer regions.
It has the property of attenuating the energy of the radiation to 1/100 or less when the radiation passes through the support.

In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet is separated by a distance equal to the distance between adjacent photostimulable phosphor layer regions.
It has the property of attenuating the energy of the radiation to 1/500 or less when the radiation passes through the support.

In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet is separated by a distance equal to the distance between adjacent photostimulable phosphor layer regions.
It has the property of attenuating the energy of the radiation to 1/1000 or less when the radiation passes through the support.

In the present invention, the material for forming the support of the stimulable phosphor sheet is preferably one having a property of attenuating radiation, and the material having a property of attenuating radiation is particularly limited. But not
Both inorganic compound materials and organic compound materials can be used, and metal materials, ceramic materials or plastic materials are particularly preferably used.

In the present invention, as the inorganic compound material which can be preferably used for forming the support of the stimulable phosphor sheet, for example, gold, silver, copper, zinc,
Metals such as aluminum, titanium, tantalum, chromium, iron, nickel, cobalt, lead, tin and selenium; alloys such as brass, stainless steel and bronze; silicon materials such as silicon, amorphous silicon, glass, quartz, silicon carbide and silicon nitride. Metal oxides such as aluminum oxide, magnesium oxide and zirconium oxide; inorganic salts such as tungsten carbide, calcium carbonate, calcium sulfate, hydroxyapatite and gallium arsenide. These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic.

In the present invention, a polymer compound is preferably used as the organic compound material that can be preferably used for forming the support of the stimulable phosphor sheet, and a preferable polymer compound is, for example, polyethylene. Polyolefin such as polypropylene and polypropylene; Acrylic resin such as polymethylmethacrylate, butyl acrylate / methylmethacrylate copolymer; Polyacrylonitrile; Polyvinyl chloride; Polyvinylidene chloride; Polyvinylidene fluoride; Polytetrafluoroethylene; Polychlorotrifluoroethylene; Polycarbonate Polyester such as polyethylene naphthalate and polyethylene terephthalate; Nylon such as nylon 6, nylon 6,6 and nylon 4,10; polyimide; polysulfone; polyphenylene Sulfide; Silicon resin such as polydiphenylsiloxane; Phenolic resin such as novolak; Epoxy resin; Polyurethane; Polystyrene; Butadiene-styrene copolymer; Cellulose, cellulose acetate, nitrocellulose, starch, calcium alginate, and hydroxypropylmethylcellulose Chitin; chitosan; sumacum; polyamides such as gelatin, collagen, keratin, and copolymers of these high molecular compounds. These may be composite materials, and may be filled with metal oxide particles, glass fibers, or the like, if desired, or may be used by blending with an organic compound material.

Generally, the higher the specific gravity is, the higher the radiation attenuating ability is. Therefore, the support of the stimulable phosphor sheet is preferably made of a compound material or a composite material having a specific gravity of 1.0 g / cm ³ or more. , Specific gravity is 1.5g / c
It is particularly preferable that it is formed of a compound material or a composite material of m ³ or more and 23 g / cm ³ or less.

The object of the present invention is also to provide a biochemical analysis unit comprising a casing and a lid member formed of a material that attenuates radiation, and an adsorption layer formed of an adsorbent material, and a photostimulable fluorescence unit. The stimulable phosphor sheet on which the body layer is formed, respectively, at least in part,
This is achieved by an exposure apparatus for a stimulable phosphor sheet, which is provided with a detection means for detecting whether or not they have mutually complementary shapes.

According to the present invention, the exposure apparatus for the stimulable phosphor sheet includes a biochemical analysis unit having an adsorption layer formed of an adsorbent material, and a stimulable phosphor layer-formed storage element. Phosphor sheet, each, at least in part, because it comprises a detection means for detecting whether or not they have a mutually complementary shape, the biochemical analysis unit and the stimulable phosphor sheet, The exposure operation is performed only when at least a part of them has shapes complementary to each other, that is, when the biochemical analysis unit and the stimulable phosphor sheet are allowed to be used together. Therefore, the specific binding substance is spotted and dropped on the adsorption layer of the biochemical analysis unit to form a plurality of spot-shaped regions separated from each other, and abnormal expression of a gene is observed. A substance derived from a living body, which is collected from a human or a healthy individual and is labeled with a radiolabeled substance, is selectively bound to a specific binding substance contained in a plurality of spot-shaped regions by hybridization, etc. , A spot-shaped region is selectively labeled with a radioactive labeling substance, and is superposed on a stimulable phosphor sheet on which a stimulable phosphor layer is formed. Exposing with a labeling substance, scanning the exposed photostimulable phosphor layer with excitation light to excite the photostimulable phosphor, and photoelectrically detecting the photostimulable light emitted from the photostimulable phosphor. Then, after generating the biochemical analysis data, from a plurality of spot-like regions of the biochemical analysis unit, the substance of biological origin is removed by rehybridization, etc. A specific binding substance that was collected from a person whose expression was found to be abnormal and was labeled with a radiolabeling substance, and then the substance of biological origin was selectively included in multiple spot-like regions again by hybridization. Specifically labeled to the spot-shaped region with a radioactive labeling substance, and the stimulable phosphor layer formed on the stimulable phosphor sheet is a radioactive labeling substance contained in the spot-shaped region. When generating data for biochemical analysis of a healthy person or a person with abnormal expression of a gene by exposure, the unit for biochemical analysis is used to generate data of a person with a abnormal expression of a gene or a healthy person. Used when the data for biochemical analysis was generated, and the stimulable phosphor sheet from which the accumulated radiation energy was erased was reliably selected and the biochemical analysis user It can be exposed using a knit and therefore
The same specific binding substance was dropped on the biochemical analysis unit to form spot-like regions having the same pattern, and the stimulable phosphor layer was uniformly formed on the stimulable phosphor sheet. Even if it is not possible, it is possible to significantly improve the accuracy of testing for abnormal gene expression.

[0085] In a preferred aspect of the present invention, the detection means has at least one penetrating member formed in the biochemical analysis unit and the stimulable phosphor sheet so as to have complementary shapes to each other. It is configured to detect light transmitted through the hole.

In another preferred aspect of the present invention, at least one of the detection means is formed in the biochemical analysis unit and the stimulable phosphor sheet so as to have complementary shapes to each other. It is configured to detect one notch.

In a further preferred aspect of the present invention, the exposure apparatus for the stimulable phosphor sheet further comprises: the biochemical analysis unit and the stimulable phosphor sheet.
When it is determined that the biochemical analysis unit and the stimulable phosphor sheet do not have complementary shapes to each other, a display means is provided for displaying that both the biochemical analysis unit and the stimulable phosphor sheet cannot be used.

In another preferred embodiment of the present invention, the stimulable phosphor sheet exposure apparatus further comprises: the biochemical analysis unit and the stimulable phosphor sheet.
There is provided alarm means for generating an alarm sound when it is determined that the shapes do not have mutually complementary shapes.

In a further preferred aspect of the present invention, the lid member is further determined when it is determined that the biochemical analysis unit and the stimulable phosphor sheet do not have complementary shapes to each other. It is provided with a locking means for locking so that it cannot be closed.

According to a further preferred embodiment of the present invention, the biochemical analysis unit and the stimulable phosphor sheet set in the exposure apparatus do not have mutually complementary shapes and can be used together. When it is not allowed, the lid member cannot be closed and the exposure operation cannot be executed.Therefore, the specific binding substance is spotted on the adsorption layer of the biochemical analysis unit. Droplets are formed to form multiple spot-shaped regions that are separated from each other, and are collected from humans or healthy individuals with abnormal gene expression, and biologically-derived substances labeled with radioactive labeling substances are selected by hybridization, etc. Specifically, by specifically binding to specific binding substances contained in a plurality of spot-shaped regions and selectively spot-shaped regions by a radiolabeled substance. When the stimulable phosphor layer is formed, the stimulable phosphor layer is superposed on the stimulable phosphor sheet and exposed by a radioactive labeling substance selectively contained in a plurality of spot-shaped regions to expose the stimulable phosphor layer. Scanning the phosphor layer by excitation light, to excite the stimulable phosphor, photoelectrically detecting the stimulable light emitted from the stimulable phosphor, after generating the data for biochemical analysis, From multiple spot-shaped areas of the biochemical analysis unit, substances derived from the living body are removed by rehybridization, etc., and collected from a healthy person or a person whose gene expression abnormality is observed, and the body is labeled with a radiolabeled substance. The derived substance is selectively bound again to the specific binding substance contained in the plurality of spot-shaped regions by hybridization or the like, and then spotted by the radiolabeled substance. Area is selectively labeled, and the stimulable phosphor layer formed on the stimulable phosphor sheet is exposed by a radioactive labeling substance contained in the spot-shaped area, which causes abnormal expression in healthy individuals or genes. When generating data for biochemical analysis of an authorized person,
Using the biochemical analysis unit, when the data for biochemical analysis of a person or a healthy person whose gene expression abnormality is recognized is used, a stimulable phosphor sheet from which accumulated radiation energy has been erased, It can be reliably selected and exposed using the biochemical analysis unit. Therefore, the same specific binding substance is dropped on the biochemical analysis unit to form spot-like regions having the same pattern. Cannot be done, and the stimulable phosphor sheet
Even if the stimulable phosphor layer cannot be formed uniformly,
It is possible to significantly improve the accuracy of the test for abnormal gene expression.

[0091]

Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

FIG. 1 is a schematic perspective view of a biochemical analysis unit according to a preferred embodiment of the present invention.

As shown in FIG. 1, the biochemical analysis unit 1 according to the present embodiment comprises a substrate 2 formed of aluminum and having a large number of substantially circular through holes formed at high density. Nylon 6 is filled inside the large number of through holes to form a large number of dot-shaped absorptive regions 3.

Although not shown exactly in FIG. 1, in this embodiment, through holes having a size of about 10,000 square millimeters of about 10,000 are regularly formed at a density of about 5000 holes / cm 2. , Formed on the substrate 2. The absorptive region 3 is formed by filling a large number of through holes with nylon 6 so that the surface thereof is located at the same height as the surface of the substrate 2.

As shown in FIG. 1, the substrate 2 of the biochemical analysis unit 1 is provided with four matching determination through holes 4 in parallel with one edge of the substrate 2 and parallel with the opposite edge. Two positioning through holes 5 are formed in the.

Here, the positioning through hole 5 includes the biochemical analysis unit 1 and a stimulable phosphor sheet described later.
When the stimulable phosphor layer region of the stimulable phosphor sheet is exposed by the radioactive labeling substance contained in the absorptive region 3 of the biochemical analysis unit 1 after being set in the exposure apparatus by overlapping. , The biochemical analysis unit 1 and the stimulable phosphor sheet are guaranteed to be set at a predetermined position in the exposure apparatus in a predetermined positional relationship.

Further, as will be described later, in this embodiment, the biochemical analysis unit 1 uses the radioactive labeling substance selectively contained in the adsorptive region 3 to cause the stimulable fluorescence of the stimulable phosphor sheet. In the case of exposing the body layer, in order to improve the inspection accuracy of gene expression abnormality, only the specific stimulable phosphor sheet can be exposed, and four through holes for matching judgment are used. 4 is therefore formed on the substrate 2.

FIG. 2 is a schematic front view of the spotting device.

In the biochemical analysis, as shown in FIG. 2, in a large number of absorptive regions 3 regularly formed in the biochemical analysis unit 1, for example, a base sequence as a specific binding substance is used. Multiple known different cDNAs
Are dropped using a spotting device.

As shown in FIG. 2, the spotting device comprises an injector 6 and a CCD camera 7, and a CCD
The tip of the injector 6 and the specific binding substance should be dropped while observing the tip of the injector 6 and the absorptive region 3 of the biochemical analysis unit 1 on which the specific binding substance should be dropped by the camera 7. When the center of the absorptive region 3 coincides, the injector 6 is configured to drop specific binding substances such as a plurality of cDNAs having different known base sequences from each other. Within the many dot-shaped absorptive regions 3, a specific binding substance,
Guaranteed to be able to drip accurately.

FIG. 3 is a schematic vertical sectional view of the hybridization container.

As shown in FIG. 3, the hybridization container 8 has a rectangular cross section, and contains a hybridization solution 9 containing a substance derived from a living body which is a probe labeled with a labeling substance. .

When a specific binding substance such as cDNA is selectively labeled with a radiolabeling substance, a hybridization solution 9 containing a substance derived from a living body which is a probe labeled with the radiolabeling substance is prepared and It is housed in the hybridization container 8.

On the other hand, by using a labeling substance which causes chemiluminescence by contacting with a chemiluminescent substrate, the cDNA is
In the case of selectively labeling a specific binding substance such as, a hybridization solution 9 containing a substance derived from a living body, which is a probe labeled with a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate, is prepared. And is housed in the hybridization container 8.

Further, in the case of selectively labeling a specific binding substance such as cDNA with a fluorescent substance such as a fluorescent dye, a high-molecular substance containing a living body which is a probe labeled with a fluorescent substance such as a fluorescent dye is used. A hybridization solution 9 is prepared and housed in the hybridization container 8.

Biogenic substance labeled with a radiolabeling substance, biogenic substance labeled with a labeling substance that produces chemiluminescence by contacting with a chemiluminescent substrate, and biomolecule labeled with a fluorescent substance such as a fluorescent dye It is also possible to prepare a hybridization solution 9 containing two or more substances derived from the living body among the substances derived from the living body, and store the hybridization solution 9 in the hybridization container 8. In the present embodiment, the living body labeled with the radioactive labeling substance is used. A hybridization solution 9 containing a substance of biological origin and a substance of biological origin labeled with a fluorescent substance such as a fluorescent dye is prepared and housed in the hybridization container 8.

Upon hybridization, cD
The biochemical analysis unit 1 in which a specific binding substance such as NA is adsorbed on a large number of absorptive regions 3 is inserted into the hybridization container 8.

As a result, the specific binding substance adsorbed on the large number of adsorptive regions 3 is separated from the biologically-derived substance labeled with a radioactive labeling substance and the biologically-derived substance labeled with a fluorescent substance such as a fluorescent dye. , Selectively, hybridized.

In this way, the radiation data, the chemiluminescence data, and the fluorescence data of the fluorescent substance such as the fluorescent dye of the labeling substance are recorded in the many absorptive regions 3 of the biochemical analysis unit 1. The fluorescence data recorded in the absorptive region 3 is read by a scanner described later to generate biochemical analysis data.

On the other hand, the radiation data of the radiolabeled substance is
The radiation data transferred to the stimulable phosphor sheet and transferred to the stimulable phosphor sheet is read by a scanner described later to generate biochemical analysis data.

FIG. 4 is a schematic perspective view of a stimulable phosphor sheet according to a preferred embodiment of the present invention.

As shown in FIG. 4, the stimulable phosphor sheet 10 according to this embodiment includes a nickel support 11 in which a large number of substantially circular through holes are regularly formed. A large number of stimulable phosphor layers 12 are embedded in the large number of through-holes formed in 1. to form a large number of stimulable phosphor layer regions 12.

A large number of through holes are provided in the biochemical analysis unit 1.
Of the stimulable phosphor layer region 12 formed on the support 11 in the same pattern as the large number of the absorptive regions 3 formed on the substrate 2 of the biochemical analysis unit 1. It has the same size as the formed large number of absorptive regions 3.

Therefore, although not shown exactly in FIG. 4, in the present embodiment, about 0.
A substantially circular stimulable phosphor layer region 12 having a size of 01 square millimeters is dot-shaped on the support 11 of the stimulable phosphor sheet 10 at a density of about 5000 pieces / square centimeter and in a regular pattern. It is formed into a shape.

Further, in this embodiment, the support 11
Surface and dot-shaped stimulable phosphor layer region 1
2 so that the surface of the support 2 and the surface of the support 2 are at the same height.
The stimulable phosphor is embedded in the through hole formed in
The stimulable phosphor sheet 10 is formed.

As shown in FIG. 4, in the support 11 of the stimulable phosphor sheet 10, three matching determination through holes 13 are formed in parallel to one edge, and are parallel to the opposite edge. Two positioning through-holes 14 are formed in the.

Here, the two positioning through holes 14 are
It is formed at a position corresponding to the positioning through hole 5 formed in the substrate 2 of the biochemical analysis unit 1.

On the other hand, the three matching determination through holes 13
Is a radioactive labeling substance selectively contained in the adsorptive region 3 of the biochemical analysis unit 1 in cooperation with the four matching determination through holes 4 formed in the substrate 2 of the biochemical analysis unit 1. Therefore, when exposing the stimulable phosphor layer region 12 formed on the stimulable phosphor sheet 10, the biochemical analysis unit 1 cannot expose anything other than the specific stimulable phosphor sheet 10. It has a function of improving the inspection accuracy of gene expression abnormality, and is provided at a position complementary to the four matching determination through holes 4 formed in the substrate 2 of the biochemical analysis unit 1. Has been formed.

That is, the stimulable phosphor sheet 10 shown in FIG. 4 is a substrate of the biochemical analysis unit 1 when the biochemical analysis unit 1 is superposed and set in an exposure apparatus described later. The four matching determination through-holes 4 formed in 2 form the support 11 of the stimulable phosphor sheet 10.
And the support 11 of the stimulable phosphor sheet 10
The four matching determination through holes 4 of the biochemical analysis unit 1 and the accumulation are formed in such a positional relationship that the three matching determination through holes 13 formed in the above are blocked by the substrate 2 of the biochemical analysis unit 1. Three through holes 13 for matching determination of the fluorescent phosphor sheet 10 are formed.

FIG. 5 is a schematic perspective view of a stimulable phosphor sheet exposure apparatus according to a preferred embodiment of the present invention, and FIG. 6 is a schematic partial sectional view thereof.

As shown in FIGS. 5 and 6, the exposure apparatus for the stimulable phosphor sheet according to the present embodiment mounts the casing 15, the biochemical analysis unit 1 and the stimulable phosphor sheet 10. Support plate 16 and casing 15
A lid member 17 that can be opened and closed around a shaft 15a formed in
And two positioning pins 1 formed on the support plate 16.
8, 18 and the transparent glass plate 19 formed on the support plate 16
And a fluorescent lamp 20 provided inside the casing 15 below the transparent glass plate 19.

The lid member 17 keeps the inside of the casing 15 in a light-shielded state when closed.
The casing 15 and the lid member 17 attached to the casing 15 are made of metal having a property of attenuating radiation.

Radioactive label in which the dot-shaped stimulable phosphor layer region 12 formed on the stimulable phosphor sheet 10 is included in the dot-shaped absorptive region 3 formed in the biochemical analysis unit 1. When exposing with a substance, first, the biochemical analysis unit 1 and the stimulable phosphor sheet 10 are superposed on each other, and the through hole 5 for positioning and the stimulable fluorescence formed in the substrate 2 of the biochemical analysis unit 1 are stacked. Body sheet 10
In the positioning through hole 14 formed in the support 11 of
Positioning pin 1 formed on the support 16 of the exposure apparatus
8 and 18 are set on the support plate 16 of the exposure apparatus so as to be inserted therethrough.

In this way, the biochemical analysis unit 1
The positioning pins 18, 18 formed on the support 16 of the exposure apparatus are set on the support plate 16 of the exposure apparatus so that the positioning pins 18, 18 formed on the support 16 of the exposure apparatus are inserted into the through-holes 5 for positioning that are formed in the substrate 2, and the accumulation property is improved. The phosphor sheet 10 is provided with a support plate 16 of the exposure apparatus so that the positioning pins 18, 18 formed on the support 16 of the exposure apparatus are inserted into the positioning through holes 14 formed in the support 11. Since it is set on the top, the biochemical analysis unit 1 and the stimulable phosphor sheet 10 can be set at desired positions on the support plate 16 of the exposure apparatus.

When the biochemical analysis unit 1 and the stimulable phosphor sheet 10 are set in the exposure device, the fluorescent lamp 20 is turned on.

In this embodiment, the biochemical analysis unit 1 is used when the stimulable phosphor layer of the stimulable phosphor sheet is exposed to the radioactive labeling substance selectively contained in the adsorptive region 3. Is configured so that it cannot be used except for exposing a specific stimulable phosphor sheet 10 in order to improve the inspection accuracy of gene expression abnormality, and the fluorescent lamp 20 is set in the exposure device. The light is turned on to determine whether the biochemical analysis unit 1 and the stimulable phosphor sheet 10 can be used together.

FIG. 7 shows the substrate 2 of the biochemical analysis unit 1.
FIG. 3 is a schematic plan view for explaining a relative positional relationship between a matching determination through hole 4 formed in 1 and a matching determination through hole 13 formed in a support 11 of the stimulable phosphor sheet 10.

As shown in FIG. 7, the substrate 2 of the biochemical analysis unit 1 and the support 11 of the stimulable phosphor sheet 10 are provided with seven through holes 4 and 13 for matching determination.
The substrate 2 of the biochemical analysis unit 1 shown in FIG. 1, which is configured so as to be formed regularly, has four matching determination through holes 4 formed therein, and the accumulation fluorescence shown in FIG. The support 11 of the body sheet 10 has three matching determination through holes 13 formed therein.

In this embodiment, as shown in FIG. 7A, when the biochemical analysis unit 1 and the stimulable phosphor sheet 10 are superposed, the substrate 2 of the biochemical analysis unit 1 is stacked. All of the matching determination through holes 4 formed in the above are closed by the support 11 of the stimulable phosphor sheet 10, and the matching determination through holes 13 formed in the support 11 of the stimulable phosphor sheet 10 are formed. Is blocked only by the substrate 2 of the biochemical analysis unit 1, that is, the light emitted from the fluorescent lamp 20 is absorbed by the biochemical analysis unit 1 and the stimulable phosphor sheet 10.
It is allowed to use both the biochemical analysis unit 1 and the stimulable phosphor sheet 10 only when the biochemical analysis unit 1 is blocked, and as shown in FIG. 7B, the substrate of the biochemical analysis unit 1 is used. Matching determination through-hole 4 formed in 2
And even if one of the matching determination through-holes 13 formed in the support 11 of the stimulable phosphor sheet 10 has the same position, that is, the biochemical analysis unit 1
The substrate 2 of the biochemical analysis unit 1 when the stimulable phosphor sheet 10 and the stimulable phosphor sheet 10 are stacked and set in an exposure apparatus.
The light emitted from the fluorescent lamp 20 passes through at least one of the matching-determination through-holes 4 formed in 1 and at least one of the matching-determination through-holes 13 formed in the support 11 of the stimulable phosphor sheet 10. When transmitted, the biochemical analysis unit 1 and the stimulable phosphor sheet 10 are prohibited from being used together.

Biochemical analysis unit 1 shown in FIG.
And the stimulable phosphor sheet 10 shown in FIG. 4 are overlapped with each other, and when set in the exposure apparatus, four matching determination through holes formed in the substrate 2 of the biochemical analysis unit 1 4 is closed by the support 11 of the stimulable phosphor sheet 10, and the three matching determination through holes 13 formed in the support 11 of the stimulable phosphor sheet 10 are the substrates of the biochemical analysis unit 1. Since the four matching determination through holes 4 of the biochemical analysis unit 1 and the three matching determination through holes 13 of the stimulable phosphor sheet 10 are formed in such a positional relationship that they are blocked by 2, The light emitted from the lamp 20 is the substrate of the biochemical analysis unit 1 formed of aluminum and the support 1 of the stimulable phosphor sheet 10 formed of nickel.
1 does not impinge on the eyes of the user, and therefore, the user can see that the biochemical analysis unit 1 shown in FIG. 1 and the stimulable phosphor sheet 10 shown in FIG. You can know that it is acceptable to use.

On the other hand, as shown in FIG. 7B, the matching determination through-hole 4 formed in the substrate 2 of the biochemical analysis unit 1 and the support 11 of the stimulable phosphor sheet 10. If even one of the matching determination through-holes 13 formed in the above-mentioned case has the same position, the fluorescent lamp 2
The light emitted from 0, through holes for matching determination 4 formed in the substrate 2 of the biochemical analysis unit 1 and through holes for matching determination 13 formed in the support 11 of the stimulable phosphor sheet 10. The user knows that the biochemical analysis unit 1 and the stimulable phosphor sheet 10 are prohibited from being used together by sensing the transmitted light. Can
The biochemical analysis unit 1 and the stimulable phosphor sheet 10 are removed from the exposure apparatus, and another stimulable phosphor sheet and the biochemical analysis unit 1 are superposed and set in the exposure apparatus.

Therefore, a specific binding substance was dropped onto a large number of absorptive regions 3 of the biochemical analysis unit 1 and collected from a person in which gene expression abnormality was recognized or a healthy person,
A substance of biological origin labeled with a radioactive labeling substance,
By selectively binding specifically binding substances contained in a large number of absorptive regions 3 by hybridization or the like, the absorptive regions 3 are selectively labeled with a radioactive labeling substance, and a large number of bright A large number of stimulable phosphor layer regions are formed by stacking the stimulable phosphor layer regions 12 on the stimulable phosphor sheet 10 and by using the radioactive labeling substance selectively contained in the large number of absorptive regions 3. 12 is exposed, and the exposed photostimulable phosphor layer region 12 is scanned with excitation light,
After exciting the photostimulable phosphor and photoelectrically detecting the photostimulable light emitted from the photostimulable phosphor to generate biochemical analysis data, a large number of adsorptions of the biochemical analysis unit 1 are performed. The biogenic substance is removed from the sex region 3 by rehybridization, and the biogenic substance labeled with a radiolabeled substance is collected again from the healthy subject or a person whose gene expression abnormality is recognized by hybridization. Selectively binding the specific binding substance contained in a large number of the absorptive regions 3 selectively, and selectively labeling the absorptive region 3 with a radioactive labeling substance, and A large number of stimulable phosphor layer regions 12 formed
By the radioactive labeling substance contained in the adsorptive region 3,
When generating data for biochemical analysis of a healthy person or a person with abnormal expression of a gene by exposure, the unit 1 for biochemical analysis is used to generate data of a person with a abnormal expression of a gene or a healthy person. The biochemical analysis unit 1 is used for exposing a stimulable phosphor sheet 10 other than the stimulable phosphor sheet 10 used when the biochemical analysis data is generated.
Can not be used, and when the biochemical analysis unit 1 is used to generate biochemical analysis data of a person with a gene expression abnormality or a healthy person, the accumulated radiation energy is deleted. The biochemical analysis unit 1 for surely selecting the stored stimulable phosphor sheet 10
Can be used to expose the stimulable phosphor layer region 12 of the stimulable phosphor sheet 10, so that the same biochemical analysis unit 1 and the same stimulable phosphor sheet 10 can be used to cause abnormal gene expression. Therefore, it is difficult to form the absorptive region 3 having the same pattern on the biochemical analysis unit 1, and the stimulable phosphor sheet 10 has a large number of stimulable phosphors. Even if it is difficult to form the layer region 12 uniformly, it is possible to significantly improve the accuracy of inspection for abnormal gene expression.

FIG. 8 shows a large number of dot-shaped stimulants formed on the stimulable phosphor sheet 10 by the radioactive labeling substance contained in the large number of absorptive regions 3 formed in the biochemical analysis unit 1. FIG. 6 is a schematic cross-sectional view showing a method of exposing the phosphor layer region 12 to light.

As shown in FIG. 8, upon exposure,
The many absorptive regions 3 formed in the biochemical analysis unit 1 face the many dot-shaped stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10. The stimulable phosphor sheet 10 and the biochemical analysis unit 1 are superposed.

In the present embodiment, since the biochemical analysis unit 1 is formed by filling nylon 6 in a large number of through holes formed in the aluminum substrate 2, hybridization etc. It undergoes almost no expansion and contraction when treated with liquid, and therefore
In order that the many absorptive regions 3 formed in the biochemical analysis unit 1 exactly face the many dot-shaped stimulable phosphor layer regions 12 formed in the stimulable phosphor sheet 10, Storage phosphor sheet 10 and biochemical analysis unit 1
The dot-shaped stimulable phosphor layer regions 12 can be exposed by easily and surely superimposing the above.

Thus, over a predetermined period of time, each of a large number of dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 and a large number of adsorption formed on the biochemical analysis unit 1. By facing the absorptive region 3, a large number of dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 are exposed by the radioactive labeling substance contained in the absorptive region 3.

At this time, an electron beam is emitted from the radioactive labeling substance adsorbed on the adsorbent region 3, but the adsorbent region 3 of the biochemical analysis unit 1 is separated from the substrate 2 formed of aluminum. Aluminum which has a property of attenuating radiation is present around each of the absorptive regions 3 formed in a dot shape, and further, a large number of dot-shaped stimulability of the stimulable phosphor sheet 10 are present. The phosphor layer region 12 is formed by embedding the stimulable phosphor 11 in a plurality of through holes formed in the nickel support 11 having a property of attenuating radiation, and each of the stimulable phosphors is formed. Since the nickel-made support 11 having a property of attenuating radiation is present around the layer region 12, the electron beam emitted from the radiolabel substance contained in the adsorptive region 3 is scattered. Therefore, all the electron beams emitted from the radiolabeled substance contained in the adsorptive region 3 are incident on the stimulable phosphor layer region 12 facing the adsorptive region 3. Thus, it is possible to reliably prevent the light from entering the stimulable phosphor layer region 12 to be exposed by the electron beam emitted from the adsorbing regions 3 adjacent to each other.

Therefore, the large number of dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 are only the radioactive labeling substances contained in the corresponding absorptive regions 3 of the biochemical analysis unit 1. By this, it becomes possible to reliably perform exposure.

In this way, the radiation data of the radioactive labeling substance is recorded in the large number of dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10.

In FIG. 9, the radiation data recorded in the stimulable phosphor layer area of the stimulable phosphor sheet 10 and the fluorescence data recorded in the absorptive area 3 of the biochemical analysis unit 1 are read to obtain the raw data. FIG. 11 is a schematic perspective view of a scanner for generating data for chemical analysis, and FIG. 10 is a schematic perspective view showing details of the scanner near the photomultiplier.

The scanner according to this embodiment is a unit for radiation data and biochemical analysis of radiolabeled substances recorded in a large number of dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10. Fluorescent data such as fluorescent dyes recorded in a large number of absorptive regions 3 can be read.

As shown in FIG. 9, the scanner according to this embodiment includes a first laser excitation light source 21 which emits a laser light 24 having a wavelength of 640 nm and a second laser excitation light source 21 which emits a laser light 24 having a wavelength of 532 nm. Excitation light source 22 and 47
It is provided with a third laser excitation light source 23 which emits a laser beam 24 having a wavelength of 3 nm.

In the present embodiment, the first laser excitation light source 21 is composed of a semiconductor laser light source, and the second laser excitation light source 22 and the third laser excitation light source 23.
Is composed of a second harmonic generation element.

The laser light 24 generated by the first laser excitation light source 21 is collimated by the collimator lens 25 and then reflected by the mirror 26. First
In the optical path of the laser light 24 emitted from the laser excitation light source 21 and reflected by the mirror 26, the first dichroic mirror 27 that transmits the laser light 4 of 640 nm and reflects the light of 532 nm wavelength and the 532 nm or longer A second dichroic mirror 28 that transmits light of a wavelength and reflects light of a wavelength of 473 nm is provided.
Laser light 24 generated by the laser excitation light source 21 of
Passes through the first dichroic mirror 27 and the second dichroic mirror 28 and enters the mirror 29.

On the other hand, the laser light 24 generated by the second laser excitation light source 22 is passed by the collimator lens 30.
After being made into parallel light, it is reflected by the first dichroic mirror 27, its direction is changed by 90 degrees, and
The light passes through the dichroic mirror 28 and enters the mirror 29.

The laser light 24 generated from the third laser excitation light source 23 is collimated by the collimator lens 31, and then the second dichroic mirror 2 is used.
After being reflected by 8 and changing its direction by 90 degrees,
It is incident on the mirror 29.

The laser light 24 incident on the mirror 29 is reflected by the mirror 29, and further incident on the mirror 32 and reflected.

Laser light 2 reflected by the mirror 32
A perforated mirror 34 formed by a concave mirror having a hole 33 formed in the center is arranged in the optical path of No. 4, and the laser light 24 reflected by the mirror 32 passes through the hole 33 of the perforated mirror 34. It passes through and enters the concave mirror 38.

Laser light 24 incident on the concave mirror 38
Is reflected by the concave mirror 38, and the optical head 3
It is incident on 5.

The optical head 35 is provided with a mirror 36 and an aspherical lens 37. The laser light 24 incident on the optical head 35 is reflected by the mirror 36, and the aspherical lens 37 causes the glass plate of the stage 40 to be reflected. It is incident on the stimulable phosphor sheet 10 or the biochemical analysis unit 1 placed on 41. When the biochemical analysis unit 1 is set on the stage 40, the biochemical analysis unit 1 is placed on the glass plate 41 of the stage 40 so that the surface of the absorptive region 3 faces downward. .

Laser light 24 is applied to the stimulable phosphor sheet 10.
Is incident, a large number of dot-shaped stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 are excited, stimulable light 45 is emitted, and the biochemical analysis unit is also used. When the laser light 24 is incident on 1, the fluorescent substance such as a fluorescent dye contained in a large number of the absorptive regions 3 is excited and fluorescence 45 is emitted.

Photostimulable light 45 emitted from a large number of dot-shaped stimulable phosphor layer regions 12 of the stimulable phosphor sheet 10 or fluorescence emitted from a large number of absorptive regions 3 of the biochemical analysis unit 1. 45 is condensed on a mirror 36 by an aspherical lens 37 provided in the optical head 35, reflected on the same side as the optical path of the laser beam 24 by the mirror 36, becomes parallel light, and is reflected by a concave mirror 38. Incident.

The photostimulable light 45 or the fluorescence 45 incident on the concave mirror 38 is reflected by the concave mirror 38,
The light enters the perforated mirror 34.

The photostimulable light 45 or the fluorescence 45 incident on the perforated mirror 34 is reflected downward by the perforated mirror 34 formed by a concave mirror to enter the filter unit 48, as shown in FIG. Then, light of a predetermined wavelength is cut off, enters the photomultiplier 50, and is detected photoelectrically.

As shown in FIG. 10, the filter unit 48 includes four filter members 51a, 51b, 51.
c and 51d, the filter unit 48 is configured to be movable in the left-right direction in FIG. 10 by a motor (not shown).

FIG. 11 is a schematic sectional view taken along the line AA of FIG.

As shown in FIG. 11, the filter member 5
1a includes a filter 52a, and the filter 52a includes a first
Is a filter member used when exciting a fluorescent substance contained in a large number of absorptive regions 3 formed in the biochemical analysis unit 1 by using the laser excitation light source 21 of FIG. Cut the light of 640 nm wavelength,
It has a property of transmitting light having a wavelength longer than 640 nm.

FIG. 12 is a schematic sectional view taken along the line BB of FIG.

As shown in FIG. 12, the filter member 5
1b includes a filter 52b, and the filter 52b includes a second
Is a filter member used when exciting a fluorescent substance contained in a large number of absorptive regions 3 formed in the biochemical analysis unit 1 by using the laser excitation light source 22 of FIG. Cuts light with a wavelength of 532 nm,
It has a property of transmitting light having a wavelength longer than 532 nm.

FIG. 13 is a schematic sectional view taken along the line CC of FIG.

As shown in FIG. 13, the filter member 5
1c includes a filter 52c, and the filter 52c includes a third filter 52c.
Is a filter member used when the fluorescent substance contained in the large number of absorptive regions 3 formed in the biochemical analysis unit 1 is excited by using the laser excitation light source 23 of FIG. , 473 nm is cut off, and light with a wavelength longer than 473 nm is transmitted.

FIG. 14 is a schematic sectional view taken along the line DD of FIG.

As shown in FIG. 14, the filter member 5
1d includes a filter 52d, and the filter 52d includes a first
The stimulable phosphor sheet 1 using the laser excitation light source 21 of
A large number of dot-shaped stimulable phosphor layer regions 1
2 is a filter used when exciting the photostimulable phosphor layer region 12 and reading the photostimulable light 45 emitted from the photostimulable phosphor layer region 12, and the photostimulable light 45 emitted from the photostimulable phosphor layer region 12
Has a property of transmitting only the light in the wavelength range of, and cutting the light of the wavelength of 640 nm.

Therefore, depending on the laser excitation light source to be used, the filter members 51a, 51b, 51c, 51d.
Is selectively located in front of the photomultiplier 50, the photomultiplier 50 can photoelectrically detect only the light to be detected.

The photomultiplier 50 photoelectrically detects the stimulated emission 45, and the generated analog signal is output to the A / D converter 53 and digitized by the A / D converter 53. , Biochemical analysis data is generated and output to the data processing device 54.

On the other hand, the photomultiplier 50 photoelectrically detects the fluorescence 45, and the generated analog signal is output to the A / D converter 53 and digitized by the A / D converter 53. , Biochemical analysis data is generated.

FIG. 15 is a schematic plan view of the scanning mechanism of the optical head 35. In FIG. 15, for simplification, the optical system excluding the optical head 35 and the optical paths of the laser light 24 and the fluorescent light 45 or the stimulated light 45 are omitted.

As shown in FIG. 15, the optical head 35
The scanning mechanism that scans the substrate includes a substrate 60, and a sub-scanning pulse motor 61 and a pair of rails 62, 62 are provided on the substrate 60.
And 6 are fixed on the substrate 60 and are movable in the sub-scanning direction indicated by arrow Y in FIG.
3 and 3 are provided.

A threaded hole (not shown) is formed in the movable substrate 63, and a threaded rod 64 rotated by the sub-scanning pulse motor 61 is formed in this hole. Engaged.

A main scanning stepping motor 65 is provided on the movable substrate 63, and the main scanning stepping motor 65 attaches the endless belt 66 to the adjacent dot-shaped adsorptive properties formed in the biochemical analysis unit 1. The distance between the regions 3, that is, the distance between the adjacent dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10, is intermittently drivable. There is. The optical head 35 is fixed to the endless belt 66, and by the main scanning stepping motor 65,
When the endless belt 66 is driven, it is configured to move in the main scanning direction indicated by an arrow X in FIG. In FIG. 15, 67 is the optical head 3.
Reference numeral 5 is a linear encoder for detecting the position in the main scanning direction, and 68 is a slit of the linear encoder 67.

Therefore, the main scanning stepping motor 6
5, the endless belt 66 is intermittently driven in the main scanning direction, and when scanning of one line is completed, the substrate 63 is intermittently moved in the sub scanning direction by the sub scanning pulse motor 61. The optical head 35 is
In FIG. 15, all the dot-shaped stimulable phosphors formed on the stimulable phosphor sheet 10 by the laser beam 24 are moved in the main scanning direction indicated by the arrow X and the sub-scanning direction indicated by the arrow Y. The entire surface of the layer region 12 or the biochemical analysis unit 1 is scanned.

FIG. 16 is a block diagram showing the control system, input system, drive system and detection system of the scanner according to the preferred embodiment of the present invention.

As shown in FIG. 16, the control system of the scanner is a control unit 7 for controlling the entire scanner.
0, and the input system of the scanner is equipped with a keyboard 71 which is operated by the user and can input various instruction signals.

As shown in FIG. 16, the drive system of the scanner intermittently moves the optical head 35 in the main scanning direction and the main scanning stepping motor 65, and intermittently moves the optical head 35 in the sub scanning direction. Sub-scanning pulse motor 6
1 and 4 filter members 51a, 51b, 51c, 5
A filter unit motor 72 for moving the filter unit 48 including 1d is provided.

The control unit 70 includes a first laser pumping light source 21, a second laser pumping light source 22 or a third laser pumping light source 22.
In addition to selectively outputting a drive signal to the laser excitation light source 23, the drive signal can be output to the filter unit motor 72.

Further, as shown in FIG. 16, the detection system of the scanner comprises a photomultiplier 50 and a linear encoder 67 for detecting the position of the optical head 35 in the main scanning direction.

In the present embodiment, the control unit 70 controls the first laser pumping light source 21, the second laser pumping light source 22 or the third laser pumping light source 22 according to the position detection signal of the optical head 35 input from the linear encoder 67. The laser excitation light source 23 is configured to be on / off controllable.

The scanner according to the present embodiment configured as described above is recorded in a large number of dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 as follows. The radiation data obtained is read and biochemical analysis data is generated.

First, the stimulable phosphor sheet 10 is placed on the glass plate 41 of the stage 40.

[0180] Next, the user operates the keyboard 7
An instruction signal for scanning a large number of dot-shaped photostimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 with a laser beam 24 is input to the display device 1.

The instruction signal input to the keyboard 71 is
Input to the control unit 70, the control unit 70 outputs a drive signal to the filter unit motor 72 according to the instruction signal, and the filter unit 4
Photostimulable light 45 emitted from the photostimulable phosphor by moving 8
The filter member 51d provided with the filter 52d having the property of transmitting only the light in the wavelength range of 640 nm and cutting the light of the wavelength of 640 nm is positioned in the optical path of the stimulated emission light 45.

Further, the control unit 70 outputs a drive signal to the main scanning stepping motor 65 to move the optical head 35 in the main scanning direction, and based on the position detection signal of the optical head 35 input from the linear encoder 67. , In the first dot-shaped stimulable phosphor layer region 12,
When it is confirmed that the optical head 35 has moved to a position where the laser beam 24 can be emitted, a stop signal is output to the main scanning stepping motor 65 and a drive signal is output to the first laser excitation light source 21. Then, the first laser excitation light source 21 is activated to emit the laser light 24 having a wavelength of 640 nm.

The laser light 24 emitted from the first laser excitation light source 21 is made into parallel light by the collimator lens 25, and then enters the mirror 26 and is reflected.

Laser light 2 reflected by the mirror 26
4 passes through the first dichroic mirror 27 and the second dichroic mirror 28, and enters the mirror 29.

The laser light 24 that has entered the mirror 29 is reflected by the mirror 29, and then enters the mirror 32 and is reflected.

Laser light 2 reflected by the mirror 32
4 passes through the hole 33 of the perforated mirror 34 and enters the concave mirror 38.

The laser beam 24 incident on the concave mirror 38
Is reflected by the concave mirror 38, and the optical head 3
It is incident on 5.

Laser light 24 incident on the optical head 35
Is reflected by the mirror 36 and is condensed by the aspherical lens 37 onto the first dot-shaped stimulable phosphor layer region 12 of the stimulable phosphor sheet 10 placed on the stage 40 glass plate 41. It

As a result, the stimulable phosphor contained in the first dot-shaped stimulable phosphor layer region 12 formed on the stimulable phosphor sheet 10 is excited by the laser beam 24 to generate the first stimulable phosphor. The photostimulable light 45 is emitted from the photostimulable phosphor layer region 12.

At this time, since the support 11 of the stimulable phosphor sheet 10 is made of nickel having a property of attenuating light, the laser light 24 is scattered inside the support 11 and becomes the first. The stimulable phosphor contained in the stimulable phosphor layer region 12 adjacent to the stimulable phosphor layer region 12 is excited, and the accumulated radiation energy is emitted in the form of stimulable light 45. Can be effectively prevented, and further, the photostimulable light 45 emitted from the first photostimulable phosphor layer region 12 is scattered in the support 11 and detected by the photomultiplier 50. It becomes possible to effectively prevent that it is not done.

The photostimulable light 45 emitted from the first dot-shaped photostimulable phosphor region 12 is collected by the aspherical lens 37 provided in the optical head 35, and the laser light 24 is reflected by the mirror 36. The light is reflected on the same side as the optical path, becomes parallel light, and enters the concave mirror 38.

The photostimulable light 45 incident on the concave mirror 38 is
The perforated mirror 34 is reflected by the concave mirror 38.
Incident on.

Photostimulation 45 incident on the perforated mirror 34
Is reflected downward by the perforated mirror 34 formed by the concave mirror, and is incident on the filter 52d of the filter unit 48, as shown in FIG.

The filter 52d transmits only the light in the wavelength region of the photostimulable light 45 emitted from the photostimulable phosphor, and emits 640n.
Since it has a property of cutting off the light of wavelength m, the light of wavelength 640 nm which is the excitation light is cut off and the wavelength of photostimulable light 45 emitted from the dot-shaped photostimulable phosphor layer region 12 Only the light in the region passes through the filter 52d and is photoelectrically detected by the photomultiplier 50.

The photomultiplier 50 photoelectrically detects the photostimulable light 45, and the generated analog signal is output to the A / D converter 53 and digitized by the A / D converter 53. , Biochemical analysis data is generated and output to the data processing device 54.

When a predetermined time, for example, several microseconds has elapsed after the first laser excitation light source 21 was turned on, the control unit 70 outputs a drive stop signal to the first laser excitation light source 21, The drive of the first laser excitation light source 21 is stopped, and a drive signal is output to the main scanning stepping motor 65 so that the optical head 35 is driven by the adjacent dots formed on the stimulable phosphor sheet 10. By a pitch equal to the distance between the fluorescent phosphor layer regions 12,
To move.

Based on the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is moved by one pitch equal to the distance between the adjacent dot-shaped stimulable phosphor layer regions 12 to form the laser light 24 emitted from the first laser excitation light source 21 on the stimulable phosphor sheet 10. When it is confirmed that the second dot-shaped photostimulable phosphor layer region 12 thus irradiated has been moved to a position where it can be irradiated, the control unit 70 outputs a drive signal to the first laser excitation light source 21. The stimulability contained in the second dot-shaped stimulable phosphor layer region 12 formed on the stimulable phosphor sheet 10 by turning on the first laser excitation light source 21 and the laser light 24. Excite the phosphor.

Similarly, the laser beam 24 emitted from the first laser excitation light source 21 for a predetermined time is used as the second dot-shaped stimulable phosphor layer formed on the stimulable phosphor sheet 10. The region 12 is irradiated and the stimulable phosphor contained in the second stimulable phosphor layer region 12 is excited,
Photostimulated light 4 emitted from the second photostimulable phosphor layer region 12
5 is photoelectrically detected by the photomultiplier 50 to generate an analog signal, and the A / D converter 53
When the biochemical analysis data is generated from the radiation data digitized and recorded in the second stimulable phosphor layer region 12 by the control unit 70,
An off signal is output to the first laser excitation light source 21 to
The laser excitation light source 21 is turned off, and a drive signal is output to the main scanning stepping motor 65 so that the optical head 35 is moved to the adjacent dot-shaped stimulable phosphor layer region 1.
Move one pitch equal to the distance between two.

Thus, the first laser excitation light source 21 is repeatedly turned on and off in synchronization with the intermittent movement of the optical head 35, and the optical head 35 is detected based on the position detection signal of the optical head 35 input from the linear encoder 67. 35 is moved by one line in the main scanning direction, and when it is confirmed that the scanning of the dot-shaped stimulable phosphor layer region 12 of the first line by the laser light 24 is completed, the control unit 70 Outputs a drive signal to the main scanning stepping motor 65 to return the optical head 35 to its original position and outputs a drive signal to the sub scanning pulse motor 61 to move the movable substrate 63 to the sub scanning direction. Then, move only one line.

Based on the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is returned to its original position, and the movable substrate 63 is
When it is confirmed that the line is moved by one line in the sub-scanning direction, the control unit 70 sequentially applies the first laser excitation to the dot-shaped stimulable phosphor layer region 12 of the first line. Laser light emitted from the first laser excitation light source 21 is sequentially applied to the dot-shaped stimulable phosphor layer region 12 of the second line in the same manner as the irradiation of the laser light 24 emitted from the light source 21. 24 to irradiate 24 to excite the stimulable phosphor contained in the dot-shaped stimulable phosphor layer region 12 to sequentially emit stimulable light 45 emitted from the stimulable phosphor layer region 15. , The photomultiplier 50 is detected photoelectrically.

The photomultiplier 50 photoelectrically detects the photostimulable light 45, and the generated analog signal is output to the A / D converter 53 and digitized by the A / D converter 53. , Biochemical analysis data is generated and output to the data processing device 54.

In this way, the large number of dot-shaped photostimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 are all scanned by the laser light 24 emitted from the first laser excitation light source 21, and a large number of them are scanned. When the stimulable phosphor contained in the dot-shaped stimulable phosphor layer region 12 is excited,
The emitted stimulated light 45 is photoelectrically detected by the photomultiplier 50, and the generated analog signal is
When biochemical analysis data is generated from the radiation data digitized by the A / D converter 53 and recorded in each dot-shaped stimulable phosphor layer region 12, the control unit 70 stops driving. The signal is output to the first laser excitation light source 21, and the driving of the first laser excitation light source 21 is stopped.

On the other hand, when the fluorescence data of the fluorescent substance recorded in the many absorptive regions 3 formed in the biochemical analysis unit 1 is read to generate the biochemical analysis digital data, first, the user The biochemical analysis unit 1 is set on the glass plate 41 of the stage 40.

Next, the user operates the keyboard 7
By inputting an instruction signal for specifying the type of the fluorescent substance, which is the labeling substance, to the first laser excitation light source 2,
From the first, second, and third laser pumping light sources 22 and 23, a laser pumping light source that emits laser light 24 having a wavelength that can most efficiently excite the fluorescent substance that is the labeling substance is selected. At the same time, the light of the wavelength of the laser light 24 used for exciting the fluorescent material is cut out of the three filter members 51a, 51b, 51c,
A filter member having a property of transmitting light having a wavelength longer than that of the excitation light is selected, and by the laser light 24,
The entire surface of the biochemical analysis unit 1 is scanned, and the fluorescence 4 emitted from the fluorescent substance contained in the many absorptive regions 3
5 is photoelectrically detected by the photomultiplier 50 to generate analog data, and the A / D converter 5
In step 3, the data is digitized and biochemical analysis data is generated.

According to this embodiment, when the biochemical analysis unit 1 and the stimulable phosphor sheet 10 which are allowed to be used together are stacked and set in the exposure apparatus, the biochemical analysis is performed. The four matching determination through holes 4 formed in the substrate 2 of the analysis unit are closed by the support 11 of the stimulable phosphor sheet 10, and three through holes 4 for matching determination are formed in the support of the stimulable phosphor sheet 10. The biochemical analysis unit 1 has a positional relationship such that the matching determination through hole 13 is blocked by the substrate 2 of the biochemical analysis unit 1.
Since the four matching determination through-holes 4 and the three matching determination through-holes 13 of the stimulable phosphor sheet 10 are formed, the light emitted from the fluorescent lamp 20 is biochemical analysis formed by aluminum. The biochemical analysis unit 1 and the biochemical analysis unit 1 do not enter the eyes of the user because they are blocked by the substrate of the unit 1 and the support 11 of the stimulable phosphor sheet 10 formed of nickel. Storage phosphor sheet 10
It can be seen that and are allowed to be used together, while the biochemical analysis unit 1 and the stimulable phosphor sheet 10 which are not allowed to be used together are superposed. When set in the exposure apparatus, the light emitted from the fluorescent lamp 20 supports the through holes 4 for matching determination formed in the substrate 2 of the biochemical analysis unit 1 and the stimulable phosphor sheet 10. Since the light is transmitted through the through hole 13 for matching determination formed in the body 11, the user senses the transmitted light so that the biochemical analysis unit 1 and the stimulable phosphor sheet 10 can be used together. You can know that it is prohibited to do.

Therefore, a specific binding substance was dropped onto a large number of absorptive regions 3 of the biochemical analysis unit 1 and collected from a person in which the gene expression abnormality was recognized or a healthy person,
A substance of biological origin labeled with a radioactive labeling substance,
By selectively binding specifically binding substances contained in a large number of absorptive regions 3 by hybridization or the like, the absorptive regions 3 are selectively labeled with a radioactive labeling substance, and a large number of bright A large number of stimulable phosphor layer regions are formed by stacking the stimulable phosphor layer regions 12 on the stimulable phosphor sheet 10 and by using the radioactive labeling substance selectively contained in the large number of absorptive regions 3. 12 is exposed, and the exposed photostimulable phosphor layer region 12 is scanned with excitation light,
After exciting the photostimulable phosphor and photoelectrically detecting the photostimulable light emitted from the photostimulable phosphor to generate biochemical analysis data, a large number of adsorptions of the biochemical analysis unit 1 are performed. The biogenic substance is removed from the sex region 3 by rehybridization, and the biogenic substance labeled with a radiolabeled substance is collected again from the healthy subject or a person whose gene expression abnormality is recognized by hybridization. Selectively binding the specific binding substance contained in a large number of the absorptive regions 3 selectively, and selectively labeling the absorptive region 3 with a radioactive labeling substance, and A large number of stimulable phosphor layer regions 12 formed
By the radioactive labeling substance contained in the adsorptive region 3,
When generating data for biochemical analysis of a healthy person or a person with abnormal expression of a gene by exposure, the unit 1 for biochemical analysis is used to generate data of a person with a abnormal expression of a gene or a healthy person. The biochemical analysis unit 1 is used for exposing a stimulable phosphor sheet 10 other than the stimulable phosphor sheet 10 used when the biochemical analysis data is generated.
Can not be used, and when the biochemical analysis unit 1 is used to generate biochemical analysis data of a person with a gene expression abnormality or a healthy person, the accumulated radiation energy is deleted. The biochemical analysis unit 1 for surely selecting the stored stimulable phosphor sheet 10
Can be used to expose the stimulable phosphor layer region 12 of the stimulable phosphor sheet 10, so that the same biochemical analysis unit 1 and the same stimulable phosphor sheet 10 can be used to cause abnormal gene expression. Therefore, it is difficult to form the absorptive region 3 having the same pattern on the biochemical analysis unit 1, and the stimulable phosphor sheet 10 has a large number of stimulable phosphors. Even if it is difficult to form the layer region 12 uniformly, it is possible to significantly improve the accuracy of inspection for abnormal gene expression.

Furthermore, according to this embodiment, when a large number of dot-shaped stimulable phosphor layer regions 12 are exposed by the radioactive labeling substance contained in a large number of dot-shaped absorptive regions 3, a large number of dots are exposed. A high-energy electron beam is emitted from the radiolabeled substance contained in the absorptive region 3, but the substrate 2 of the biochemical analysis unit 1 is made of aluminum and has a property of attenuating radiation. Therefore, the electron beam emitted from the radiolabeled substance is reliably prevented from being scattered in the substrate 2, and a large number of dot-shaped stimulable phosphor layer regions 12 are provided in the biochemical analysis unit 1. The large number of absorptive regions 3 formed on the substrate 2 and the corresponding absorptive regions 3 formed on the substrate 2 of the biochemical analysis unit 1 in the same regular pattern as the support 11 are formed. Opposed to As described above, since the stimulable phosphor sheet 10 is superposed on the biochemical analysis unit 1, the electron beam emitted from the radiolabeled substance contained in each adsorptive region 3 formed on the substrate 2 Is incident only on the corresponding dot-shaped stimulable phosphor layer region 12, and since the support 11 is made of nickel that attenuates the radiation, the electron beam is stored in the stimulable phosphor sheet 10.
Scattering within the support 11 is also prevented, and therefore only the corresponding dot-shaped stimulable phosphor layer region 12 is formed by the radioactive labeling substance contained in each adsorptive region 3 formed on the substrate 2. Therefore, the region 12 of the stimulable phosphor layer to be exposed by the radioactive labeling substance contained in each absorptive region 3 is included in the adjacent absorptive regions 3. It is possible to prevent noise caused by exposure from being generated in the data for biochemical analysis due to the electron beam emitted from the radiolabeled substance, and to greatly improve the quantitativeness of biochemical analysis. It will be possible.

FIG. 17 is a schematic perspective view of an exposure apparatus for a stimulable phosphor sheet according to another preferred embodiment of the present invention.

As shown in FIG. 17, in the exposure apparatus for a stimulable phosphor sheet according to this embodiment, further,
The sensor 73 that detects the light emitted from the fluorescent lamp 20
A display panel 74 is provided on the lid member 17 and on the side surface of the casing 15.

FIG. 18 is a block diagram showing a control system, a detection system and a display system of an exposure apparatus for a stimulable phosphor sheet according to another preferred embodiment of the present invention.

As shown in FIG. 18, the control system of the exposure apparatus for the stimulable phosphor sheet according to this embodiment has a CPU 75 for controlling the entire exposure apparatus, and the detection system is emitted from the fluorescent lamp 20. A sensor 73 for detecting light is provided, and a detection signal of the sensor 73 is input to the CPU 75.

As shown in FIG. 18, the display system of the exposure apparatus for the stimulable phosphor sheet according to this embodiment comprises a display panel 74 composed of a liquid crystal panel and the like, and an alarm means 75 for issuing an alarm sound. ing.

In this embodiment, the CPU 75 outputs a display signal to the display panel 74 when the detection signal is input from the sensor 73, and the alarm means 75.
Is configured to output an alarm signal to.

Therefore, in this embodiment, when the biochemical analysis unit 1 and the stimulable phosphor sheet 10 which are not allowed to be used together are superposed and set in the exposure apparatus, the fluorescence is not detected. The light emitted from the lamp 20 is used for at least one of the matching determination through holes 4 formed in the substrate 2 of the biochemical analysis unit 1 and for the matching determination formed in the support 11 of the stimulable phosphor sheet 10. The light passes through at least one of the through holes 13 and is detected by the sensor 73, and the detection signal from the sensor 73 is
It is output to the CPU 75.

Upon receiving the detection signal from the sensor 73, the CPU 75 outputs a display signal to the display panel 74 and an alarm signal to the alarm means 75.

When the display panel 74 receives a display signal from the CPU 75, the display panel 74 displays a message indicating that the biochemical analysis unit 1 and the stimulable phosphor sheet 10 are prohibited from being used together. The alarm means 76 is
When an alarm signal is received from the CPU 75, an alarm sound is generated.

Therefore, according to this embodiment, when the biochemical analysis unit 1 and the stimulable phosphor sheet 10 are prohibited from being used together, the user is required to display the display panel 74. Since the fact can be known by the message and alarm sound displayed on the, a person who has a specific expression of a gene by dropping a specific binding substance on many absorptive regions 3 of the biochemical analysis unit 1 Alternatively, a substance derived from a living body, which is collected from a healthy subject and is labeled with a radiolabeled substance, is selectively attached to a large number of absorptive regions 3 by hybridization or the like.
Of the stimulable phosphor layer region 12 having a large number of stimulable phosphor layer regions 12 formed thereon by selectively binding the adsorptive region 3 with a radioactive labeling substance by specifically binding to the specific binding substance contained in A large number of stimulable phosphor layer regions 12 are exposed by a radioactive labeling substance which is superposed on the sheet 10 and selectively contained in a large number of absorptive regions 3, and the exposed stimulable phosphor layer regions are exposed. 12 is scanned with excitation light to excite the stimulable phosphor, photoelectrically detect the stimulable light emitted from the stimulable phosphor to generate biochemical analysis data, and From a large number of absorptive regions 3 of the chemical analysis unit 1, substances derived from a living body are removed by rehybridization, and collected from a healthy person or a person whose gene expression is abnormally expressed, and the body is labeled with a radiolabeled substance. The substance of By hybridization, it selectively binds to a specific binding substance contained in a large number of adsorptive regions 3, and the adsorptive region 3 is selectively labeled with a radiolabeling substance to form a storage phosphor. A biochemical analysis of a healthy person or a person whose gene expression abnormality is recognized by exposing a large number of stimulable phosphor layer regions 12 formed on the sheet 10 with a radiolabel substance contained in the absorptive region 3. Other than the accumulative phosphor sheet 10 used when the biochemical analysis unit 1 is used to generate biochemical analysis data for a person or a healthy person whose gene expression is abnormal Stimulable phosphor sheet 10
When the biochemical analysis unit 1 cannot be used for the exposure of, and the biochemical analysis unit 1 is used to generate biochemical analysis data of a person or a healthy person whose gene expression abnormality is recognized. The stimulable phosphor layer region of the stimulable phosphor sheet 10 is surely selected by using the biochemical analysis unit 1 for surely selecting the stimulable phosphor sheet 10 from which the accumulated radiation energy has been erased. 12 can be exposed to light, and therefore it is guaranteed that the same biochemical analysis unit 1 and the same stimulable phosphor sheet 10 are used to inspect gene expression abnormalities. In addition, even if it is difficult to form the absorptive region 3 having the same pattern and it is difficult to uniformly form a large number of stimulable phosphor layer regions 12 on the stimulable phosphor sheet 10, It is possible to greatly improve the inspection accuracy of the aberrant expression of the child.

FIG. 19 is a schematic partial cross-sectional view showing a mechanism for locking the lid member of the stimulable phosphor sheet exposure apparatus according to another embodiment of the present invention with the casing.

As shown in FIG. 19, the lid member locking mechanism for locking the lid member 17 to the casing 15 is provided on both sides of the lid member 17, one of which is shown in FIG.

As shown in FIG. 19, the locking mechanism of the lid member 17 includes a hook member 77 provided inside both side portions of the lid member 17, and the hook member 77 around the shaft 77a in a clockwise direction in FIG. Compression spring 78 to urge
And an engaging groove 79 provided inside the casing 15.

The hook member 77 is urged by the spring force of the compression spring 78 when the lid member 17 is closed, and the engaging groove 79 provided in the casing 15.
And the lid member 17 is locked.

The lid member locking mechanism further includes a lid member 17
19 are provided inside both side portions of the hook member 77 against the biasing force of the compression spring 78, and the hook member 77 is rotated around the shaft 77a.
In the above, a solenoid 80 is provided which swings counterclockwise to release the engagement between the hook member 77 and the engagement groove 79.

FIG. 20 is a block diagram showing a control system, a detection system, a drive system and a display system of an exposure apparatus for a stimulable phosphor sheet according to another embodiment of the present invention.

As shown in FIG. 20, the control system of the exposure apparatus for the stimulable phosphor sheet according to this embodiment has a CPU 75 for controlling the entire exposure apparatus, and the detection system is emitted from the fluorescent lamp 20. A sensor 73 for detecting light is provided, and a detection signal of the sensor 73 is input to the CPU 75.

As shown in FIG. 20, the display system of the exposure apparatus for the stimulable phosphor sheet according to the present embodiment includes a display panel 74 composed of a liquid crystal panel or the like, and the drive system includes a solenoid 80. ing.

In this embodiment, the CPU 75 is configured to output a drive signal to the solenoid 80 and a display signal to the display panel 74 when a detection signal is input from the sensor 73.

Therefore, in this embodiment, when the biochemical analysis unit 1 and the stimulable phosphor sheet 10 which are not allowed to be used together are superposed and set in the exposure apparatus, the fluorescence is not detected. The light emitted from the lamp 20 is used for at least one of the matching determination through holes 4 formed in the substrate 2 of the biochemical analysis unit 1 and for the matching determination formed in the support 11 of the stimulable phosphor sheet 10. The light passes through at least one of the through holes 13 and is detected by the sensor 73, and the detection signal from the sensor 73 is
It is output to the CPU 75.

When the detection signal is input, the CPU 75
The drive signal is output to the solenoid 80 and the display signal is output to the display panel 74.

As a result, the solenoid 80 is driven,
The hook member 77 is swung clockwise around the shaft 77a in FIG. 19, the engagement between the hook member 77 and the engagement groove 79 is released, and the lid member 17 is opened.

At the same time, when the display panel 74 receives a display signal from the CPU 75, it displays a message indicating that the biochemical analysis unit 1 and the stimulable phosphor sheet 10 are prohibited from being used together.

Therefore, the biochemical analysis unit 1 is not allowed to be used together by the user by mistake.
Since the lid member 17 cannot be closed when the stimulable phosphor sheet 10 and the stimulable phosphor sheet 10 are set in the exposure device, the radioactive labeling substance selectively contained in the many absorptive regions 3 of the biochemical analysis unit 1 is used. However, many stimulable phosphor layer regions 12 of the stimulable phosphor sheet 10 cannot be exposed.

In this embodiment, when the lid member 17 is opened and the biochemical analysis unit 1 and the stimulable phosphor sheet 10 which are superposed are set in the exposure apparatus, a lid member release button (not shown). When (d) is operated, a lid member opening signal is input to the CPU 75, a drive signal is output from the CPU 75 to the solenoid 80, and as a result, the solenoid 80 is driven and the hook member 77 rotates around the shaft 77a. 19, the hook member 77 and the engagement groove 79 are disengaged from each other by being swung clockwise, and the lid member 17 is opened.

According to this embodiment, even if the user does not notice an error, the biochemical analysis unit 1 and the stimulable phosphor sheet 1 are not allowed to be used together.
When 0 is set in the exposure device, the lid member 17 is closed and the exposure operation cannot be performed. Therefore, a specific binding substance is dropped onto a large number of absorptive regions 3 of the biochemical analysis unit 1 to remove the gene. Was collected from a person or a healthy person whose expression abnormality is observed, and the substance of biological origin labeled with a radiolabeled substance was subjected to hybridization, etc.
Selectively binding to the specific binding substance contained in a large number of the absorptive regions 3 and selectively labeling the absorptive region 3 with a radioactive labeling substance, and a large number of stimulable phosphor layers The stimulable phosphor layer region 12 is exposed by the radiolabeling substance selectively contained in the plurality of absorptive regions 3 in superposition with the stimulable phosphor sheet 10 in which the regions 12 are formed, The exposed photostimulable phosphor layer region 12 is scanned with excitation light to excite the photostimulable phosphor, and the photostimulable light emitted from the photostimulable phosphor is photoelectrically detected to obtain biochemistry. After generating the data for analysis, the bio-derived substance is removed by rehybridization from the large number of absorptive regions 3 of the biochemical analysis unit 1, and the substance is collected from a healthy person or a person whose gene expression abnormality is recognized. Labeled with a radioactive labeling substance And the substance derived from a living organism, once again, by hybridization, selective, and specifically coupled to form a large number of specific binding substances contained in the absorptive regions 3,
The absorptive region 3 is selectively labeled with a radioactive labeling substance, and a large number of stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 are treated with the radioactive labeling substance contained in the absorptive region 3. , When generating biochemical analysis data of a healthy person or a person whose gene expression abnormality is recognized by exposure, a person or a healthy person whose gene expression abnormality is recognized by using the biochemical analysis unit 1 Stimulable phosphor sheet 1 used when generating biochemical analysis data
The biochemical analysis unit 1 cannot be used for the exposure of the stimulable phosphor sheet 10 other than 0, and the biochemical analysis unit 1 is used to detect a gene expression abnormality in a person or a healthy person. The stimulable phosphor sheet 10 in which the accumulated radiation energy has been erased is used to be reliably selected when the biochemical analysis data is generated, and the stimulable phosphor sheet 10 is used by using the biochemical analysis unit 1. It is possible to expose 10 photostimulable phosphor layer regions 12, and thus it is guaranteed that the same biochemical analysis unit 1 and the same stimulable phosphor sheet 10 are used to test for abnormal gene expression. Therefore, it is difficult to form the absorptive region 3 having the same pattern on the biochemical analysis unit 1, and a large number of stimulable phosphor layer regions 12 are uniformly formed on the stimulable phosphor sheet 10. Even is difficult to, it is possible to greatly improve the inspection accuracy of aberrant expression of genes.

FIG. 21 is a schematic perspective view of a biochemical analysis unit according to another preferred embodiment of the present invention.

As shown in FIG. 21, the biochemical analysis unit 81 according to the present embodiment comprises a substrate 82 formed of aluminum and having a large number of substantially circular through holes formed at high density. , Inside the many through holes,
Nylon 6 is filled to form a large number of dot-shaped absorptive regions 83.

Although not accurately shown in FIG. 21, in the present embodiment, through holes having a size of about 0.01 square millimeters of about 10,000 are regularly formed at a density of about 5000 holes / square centimeter. , Formed on the substrate 82. The surface of the absorptive region 83 is the substrate 82.
Nylon 6 is filled and formed in a large number of through holes so as to be located at the same height as the surface of the.

In this embodiment, as shown in FIG. 21, four semicircular matching determination cutouts 84 are formed at one edge of the substrate 82 of the biochemical analysis unit 81 and face each other. Positioning through hole 8 parallel to the edge
5 is formed.

In the positioning through hole 85, the biochemical analysis unit 81 and a stimulable phosphor sheet to be described later are superposed and set in the exposure apparatus, and the biochemical analysis unit 81 is adsorbed. When the stimulable phosphor layer region of the stimulable phosphor sheet is exposed by the radioactive labeling substance contained in the luminescent region 83, the biochemical analysis unit 81 and the stimulable phosphor sheet have a predetermined positional relationship. Therefore, it is ensured that it is set at a predetermined position in the exposure apparatus.

The four semicircular matching determination cutouts 84 are formed to ensure that the biochemical analysis unit 81 is used only with a specific stimulable phosphor sheet.

Also in this embodiment, the biochemical analysis unit 81 is formed by the spotting device shown in FIG. 2 in the same manner as the biochemical analysis unit 1 according to the embodiment shown in FIG. Many absorptive areas 8
A specific binding substance such as cDNA is dropped onto 3 and is adsorbed.

Further, as shown in FIG. 3, a hybridization containing a hybridization solution 9 containing a substance of biological origin labeled with a radioactive labeling substance and a substance of biological origin labeled with a fluorescent substance such as a fluorescent dye. A biochemical analysis unit 80 is set in the container 8, and a specific binding substance such as cDNA adsorbed in a large number of absorptive regions 83 is labeled with a radiolabeling substance and contained in the hybridization liquid 9. The substance derived from the living body and the substance derived from the living body, which is labeled with a fluorescent substance such as a fluorescent dye, and contained in the hybridizing liquid 9 are selectively hybridized.

Thus, the biochemical analysis unit 81
Radiation data and fluorescence data are recorded.

The chemiluminescence data recorded in the biochemical analysis unit 81 is read by the scanner shown in FIGS. 10 to 16 in the same manner as in the above embodiment to generate biochemical analysis data. It

On the other hand, the biochemical analysis unit 81
The radiation data of the radio-labeled substance recorded in (1) is transferred to the stimulable phosphor sheet.

FIG. 22 is a schematic perspective view of a stimulable phosphor sheet according to another preferred embodiment of the present invention.

As shown in FIG. 22, the stimulable phosphor sheet 90 according to this embodiment includes a nickel support 91 in which a large number of substantially circular through holes are regularly formed.
The stimulable phosphor is embedded in a large number of through holes formed in the support 91, and a large number of stimulable phosphor layer regions 92 are formed.
It is formed in a dot shape.

A large number of through holes are provided in the biochemical analysis unit 8
A plurality of stimulable phosphor layer regions 92 are formed on the support 91 in the same pattern as the large number of absorptive regions 83 formed on the first substrate 82.
It has the same size as a large number of absorptive regions 83 formed on one substrate 82.

Therefore, although not accurately shown in FIG. 22, in this embodiment, a substantially circular stimulable phosphor layer region 92 having a size of about 10,000 square millimeters of about 10,000 is provided. Dots are formed on the support 91 of the stimulable phosphor sheet 90 in a regular pattern with a density of about 5000 pieces / cm <2>.

In this embodiment, the support 91
Surface and dot-shaped stimulable phosphor layer region 9
The support 91 is placed so that the surface of
The stimulable phosphor is embedded in the through hole formed in
A stimulable phosphor sheet 90 is formed.

As shown in FIG. 22, the support 11 of the stimulable phosphor sheet 10 is provided with three semi-circular matching determination notches 93 at one edge thereof, and the edge portions are opposed to each other. Two positioning through holes 94 are formed in parallel.

Here, the two positioning through holes 14 are
It is formed at a position corresponding to the positioning through hole 5 formed in the substrate 2 of the biochemical analysis unit 1.

On the other hand, the three semicircular matching determination notches 93 are the four semicircular matching determination notches 8 formed on the substrate 82 of the biochemical analysis unit 81.
When the stimulable phosphor layer region 92 of the stimulable phosphor sheet 90 is exposed by the radioactive labeling substance selectively contained in the adsorptive region 83 of the biochemical analysis unit 81 in cooperation with In order to improve the inspection accuracy of the gene expression abnormality, the biochemical analysis unit 1 guarantees that only the specific stimulable phosphor sheet 10 can be exposed, and the inspection accuracy of the gene expression abnormality is improved. The substrate 82 of the biochemical analysis unit 81, which has the function of improving
It is formed at a position complementary to the four matching determination cutouts 84 formed in.

That is, the stimulable phosphor sheet 90 shown in FIG. 22 is placed on the substrate 82 of the biochemical analysis unit 81 when the biochemical analysis unit 81 is overlaid and set in the exposure apparatus. The formed four matching determination notches 84 are the support 9 of the stimulable phosphor sheet 90.
Support 9 of the stimulable phosphor sheet 90 covered by 1.
Three matching determination notches 93 formed in 1
Of the biochemical analysis unit 81 in the positional relationship such that it is covered by the substrate 82 of the biochemical analysis unit 81.
One matching determination notch 84 and three matching determination notches 93 of the stimulable phosphor sheet 90 are formed.

FIG. 23 is a schematic perspective view of an exposure apparatus for a stimulable phosphor sheet according to another preferred embodiment of the present invention.

As shown in FIG. 23, the exposure apparatus for a stimulable phosphor sheet according to the present embodiment has a casing 10
5, a support plate 106 on which the biochemical analysis unit 81 and the stimulable phosphor sheet 90 are placed, and a casing 105.
A lid member 10 that can be opened and closed around a shaft 105a formed in
7, two positioning pins 108, 108 formed on the support plate 106, and a spring (not shown), respectively.
Thus, seven biasing members 109 that are biased toward the inside of the exposure apparatus are provided.

Also in this embodiment, the lid member 107 is used to keep the inside of the casing 105 in a light-shielding state when the lid member 107 is closed.
05, the casing 105 and the lid member 1
07 is formed of a metal having a property of attenuating radiation.

[0257] The dot-shaped stimulable phosphor layer area 92 formed on the stimulable phosphor sheet 90 is contained in the dot-shaped absorptive area 83 formed in the biochemical analysis unit 81. When exposing with a substance, first
The biochemical analysis unit 81 and the stimulable phosphor sheet 90 are overlapped with each other to form the substrate 8 of the biochemical analysis unit 81.
In the positioning through hole 85 formed in 2 and the positioning through hole 94 formed in the support 91 of the stimulable phosphor sheet 90, the positioning pins 108 and 108 formed in the support 106 of the exposure apparatus are provided. It is set on the support plate 106 of the exposure apparatus so as to be inserted.

At this time, the urging member 109 includes the biochemical analysis unit 81 and the stimulable phosphor sheet 90 which are superposed on each other.
And are pushed into the casing 105 of the exposure apparatus against the spring force of the spring.

FIG. 24 shows a matching determination notch 84 formed in a substrate 82 of a biochemical analysis unit 81,
FIG. 6 is a schematic plan view for explaining a relative positional relationship of a matching determination notch 93 formed on a support 91 of a stimulable phosphor sheet 90.

As shown in FIG. 24, the substrate 82 of the biochemical analysis unit 81 and the support 91 of the stimulable phosphor sheet 90 are regularly provided with seven matching determination notches 84 and 93, respectively. 21. Of the biochemical analysis unit 81 shown in FIG. 21, the substrate 82 of the biochemical analysis unit is formed with four matching notches 84, and the stimulable phosphor sheet shown in FIG. In the support 91 of 90, three matching notches 93 are formed among them.

In this embodiment, as shown in FIG. 24A, when the biochemical analysis unit 81 and the stimulable phosphor sheet 90 are superposed, the substrate 82 of the biochemical analysis unit 81 is stacked. All the matching determination cutouts 84 formed in the above are covered with the support 91 of the stimulable phosphor sheet 90, and the matching determination cutouts 93 formed in the support 91 of the stimulable phosphor sheet 90.
Is only covered by the substrate 82 of the biochemical analysis unit 81, the biochemical analysis unit 81
24 and the stimulable phosphor sheet 90 are allowed to be used together. On the other hand, as shown in FIG. 24 (B), a matching determination notch 84 formed on a substrate 82 of the biochemical analysis unit 81 If even one of the matching determination notches 93 formed on the support 91 of the stimulable phosphor sheet 90 is in the same position, the biochemical analysis unit 1 and the stimulable phosphor sheet 10 will be described. The use of both and is prohibited, and the lid member 107 of the exposure apparatus is configured to be held in a locked state.

That is, as shown in FIG. 24B, the matching determination notch 84 formed on the substrate 82 of the biochemical analysis unit 81 and the support 91 of the stimulable phosphor sheet 90 are formed. If even one of the matching determination cutouts 93 has the same position, the biochemical analysis unit 81 and the stimulable phosphor sheet 9 are formed.
When 0 is overlaid and set in the exposure apparatus, the corresponding biasing member 109 resists the spring force of the spring by the overlaid biochemical analysis unit 81 and the stimulable phosphor sheet 90. , The lid member 107 is not pushed into the casing 105 of the exposure apparatus, and therefore all the biasing members 109 are pushed into the casing 105 of the exposure apparatus against the spring force of the springs. To unlock
By configuring the exposure apparatus, the stimulable phosphor layer region 12 of the stimulable phosphor sheet 90 which is not allowed to be used together is included in the absorptive region 83 of the biochemical analysis unit 81. The radioactive labeling substance can reliably prevent exposure.

Therefore, a specific binding substance was dropped onto a large number of absorptive regions 83 of the biochemical analysis unit 81, and the substance was collected from a person with normal gene expression or a healthy person, and was labeled with a radioactive labeling substance. Select substances from living organisms by hybridization, etc.
By specifically binding to a specific binding substance contained in a large number of absorptive regions 83, the absorptive region 8 is provided by a radiolabeling substance.
3 is selectively labeled, and is superposed on the stimulable phosphor sheet 90 in which a large number of stimulable phosphor layer regions 92 are formed, and a radioactive labeling substance selectively contained in a large number of absorptive regions 83. To expose a large number of photostimulable phosphor layer regions 92, and scan the exposed photostimulable phosphor layer regions 92 with excitation light to excite the photostimulable phosphors. After the emitted photostimulable light is photoelectrically detected to generate biochemical analysis data, a substance derived from a living body is removed from a number of absorptive regions 83 of the biochemical analysis unit 81 by rehybridization. Then, a substance derived from a living body, which was collected from a healthy person or a person whose gene expression abnormality was observed and was labeled with a radiolabeled substance, was selectively included in a large number of absorptive regions 83 by hybridization again. A large number of stimulable phosphor layer regions 92 formed on the stimulable phosphor sheet 90 by selectively binding the absorptive region 83 with a radioactive labeling substance by specifically binding to a heterogeneous binding substance, In the case of generating biochemical analysis data of a healthy person or a person whose expression abnormality is found in a gene by exposure with a radiolabel substance contained in the absorptive region 83, the biochemical analysis unit 81 is used. For exposing a stimulable phosphor sheet 90 other than the stimulable phosphor sheet 90 used when the data for biochemical analysis of a person or a healthy person whose gene expression abnormality is recognized,
The biochemical analysis unit 81 cannot be used,
A stimulable phosphor sheet in which the accumulated radiation energy has been erased by using the biochemical analysis unit 81 when generating biochemical analysis data of a person or a healthy person whose gene expression abnormality is recognized Since 90 can be reliably selected and the stimulable phosphor layer region 92 of the stimulable phosphor sheet 90 can be exposed using the biochemical analysis unit 81, the same as the same biochemical analysis unit 81. Using the stimulable phosphor sheet 90, it is guaranteed that the gene expression abnormality is tested, and therefore the biochemical analysis unit 81 is provided with the absorptive region 83 having the same pattern.
Is difficult to form, and the stimulable phosphor sheet 90 is
Even if it is difficult to uniformly form a large number of stimulable phosphor layer regions 92, it becomes possible to greatly improve the accuracy of inspection for gene expression abnormality.

The biochemical analysis unit 8 shown in FIG.
When 1 and the stimulable phosphor sheet 90 shown in FIG. 22 are overlapped and set in the exposure apparatus, four matching determination notches formed on the substrate 82 of the biochemical analysis unit 81. 84 is covered with the support 91 of the stimulable phosphor sheet 90, and the three matching determination notches 93 formed in the support 91 of the stimulable phosphor sheet 90 are the substrates 82 of the biochemical analysis unit 81. Since all the biasing members 109 are pushed into the casing 105 and thus the lid member 107 is unlocked, the lid member 107 is closed and the absorptive region of the biochemical analysis unit 81 is covered. The radioactive labeling substance contained in 83 can expose the dot-shaped stimulable phosphor layer region 92 of the stimulable phosphor sheet 90.

Thus, the radiation data recorded in the large number of dot-shaped stimulable phosphor layer regions 12 formed on the support 91 of the stimulable phosphor sheet 90 is shown in FIG. Through the scanner shown in FIG. 16 to FIG. 16, data is read and biochemical analysis data is generated.

According to this embodiment, when the biochemical analysis unit 81 and the stimulable phosphor sheet 90, which are allowed to be used together, are superposed and set in the exposure apparatus, the biochemical analysis is performed. Substrate 82 of analysis unit 81
The four matching determination cutouts 84 formed in the above are covered with the support 91 of the stimulable phosphor sheet 90, and the three matching determination cutouts 93 formed in the support 91 of the stimulable phosphor sheet 90. However, in the positional relationship such that the biochemical analysis unit 81 is covered by the substrate 82,
Since four matching determination cutouts 84 of the biochemical analysis unit 81 and three matching determination cutouts 93 of the stimulable phosphor sheet 90 are formed, all the biasing members 109 are inside the casing 105. Pushed in to unlock lid member 107, thus
The lid member 107 is closed, and the radioactive labeling substance contained in the absorptive region 83 of the biochemical analysis unit 81
The dot-shaped photostimulable phosphor layer region 92 of the stimulable phosphor sheet 90 can be exposed, while the biochemical analysis unit 81 and the stimulable phosphor sheet 90, which are not allowed to be used together. When they are stacked and set in the exposure apparatus, as shown in FIG.
A matching determination notch 84 formed on a substrate 82 of the biochemical analysis unit 81 and a stimulable phosphor sheet 90.
Notch 9 for matching determination formed in the support 91 of
The position of at least one of the three is the same, and the corresponding biasing member 109 is exposed by the superposed biochemical analysis unit 81 and the stimulable phosphor sheet 90 against the spring force of the spring. Device casing 105
Since the lid member 107 is not unlocked, the stimulable phosphor layer region 12 of the stimulable phosphor sheet 90 is included in the absorptive region 83 of the biochemical analysis unit 81 because it is not pushed inside. The radioactive labeling substance provided can reliably prevent exposure.

Therefore, a specific binding substance was dropped onto a large number of absorptive regions 83 of the biochemical analysis unit 81, and the substance was collected from a person or a healthy person whose gene expression abnormality was recognized and labeled with a radioactive labeling substance. Select substances from living organisms by hybridization, etc.
By specifically binding to a specific binding substance contained in a large number of absorptive regions 83, the absorptive region 8 is provided by a radiolabeling substance.
3 is selectively labeled, and is superposed on the stimulable phosphor sheet 90 in which a large number of stimulable phosphor layer regions 92 are formed, and a radioactive labeling substance selectively contained in a large number of absorptive regions 83. To expose a large number of photostimulable phosphor layer regions 92, and scan the exposed photostimulable phosphor layer regions 92 with excitation light to excite the photostimulable phosphors. After the emitted photostimulable light is photoelectrically detected to generate biochemical analysis data, a substance derived from a living body is removed from a number of absorptive regions 83 of the biochemical analysis unit 81 by rehybridization. Then, a substance derived from a living body, which was collected from a healthy person or a person whose gene expression abnormality was observed and was labeled with a radiolabeled substance, was selectively included in a large number of absorptive regions 83 by hybridization again. A large number of stimulable phosphor layer regions 92 formed on the stimulable phosphor sheet 90 by selectively binding the absorptive region 83 with a radioactive labeling substance by specifically binding to a heterogeneous binding substance, In the case of generating biochemical analysis data of a healthy person or a person whose expression abnormality is found in a gene by exposure with a radiolabel substance contained in the absorptive region 83, the biochemical analysis unit 81 is used. For exposing a stimulable phosphor sheet 90 other than the stimulable phosphor sheet 90 used when the data for biochemical analysis of a person or a healthy person whose gene expression abnormality is recognized,
The biochemical analysis unit 81 cannot be used,
A stimulable phosphor sheet in which the accumulated radiation energy has been erased by using the biochemical analysis unit 81 when generating biochemical analysis data of a person or a healthy person whose gene expression abnormality is recognized Since 90 can be reliably selected and the stimulable phosphor layer region 92 of the stimulable phosphor sheet 90 can be exposed using the biochemical analysis unit 81, the same as the same biochemical analysis unit 81. Using the stimulable phosphor sheet 90, it is guaranteed that the gene expression abnormality is tested, and therefore the biochemical analysis unit 81 is provided with the absorptive region 83 having the same pattern.
Is difficult to form, and the stimulable phosphor sheet 90 is
Even if it is difficult to uniformly form a large number of stimulable phosphor layer regions 92, it becomes possible to greatly improve the accuracy of inspection for gene expression abnormality.

The present invention is not limited to the above embodiments, and various modifications can be made within the scope of the invention described in the claims, and these are also included in the scope of the present invention. It goes without saying that it is a thing.

For example, in the above-described embodiment, the biochemical analysis units 1 and 81 are formed by filling nylon 6 into a large number of through holes formed in the aluminum substrates 2 and 82. Of the biochemical analysis units 1 and 8
It is not always necessary to form the first substrate 2, 82 of aluminum. Biochemical analysis unit 1, 81
The substrates 2 and 82 are preferably made of a material that attenuates radiation, but the material is not particularly limited, and the biochemical analysis unit 1 may be made of an inorganic compound material or an organic compound material. 81 substrates 2,
82 can be formed and metallic, ceramic or plastic materials are particularly preferably used. Examples of the inorganic compound material that can be preferably used to form the substrates 2 and 82 of the biochemical analysis unit 1 and 81 and that have the property of attenuating radiation include gold, silver, copper, zinc, and aluminum. Metals such as titanium, tantalum, chromium, iron, nickel, cobalt, lead, tin and selenium; alloys such as brass, stainless steel and bronze; silicon materials such as silicon, amorphous silicon, glass, quartz, silicon carbide and silicon nitride; oxidation. Metal oxides such as aluminum, magnesium oxide, zirconium oxide;
Inorganic salts such as tungsten carbide, calcium carbonate, calcium sulfate, hydroxyapatite, gallium arsenide and the like can be mentioned. These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic. Also, the biochemical analysis unit 1,
A high molecular compound is preferably used as the organic compound material having a property of attenuating radiation, which can be preferably used for forming the substrates 2 and 82 of 81, and a preferable high molecular compound is, for example, polyethylene or Polyolefin such as polypropylene; acrylic resin such as polymethylmethacrylate or butyl acrylate / methylmethacrylate copolymer; polyacrylonitrile; polyvinyl chloride; polyvinylidene chloride; polyvinylidene fluoride; polytetrafluoroethylene; polychlorotrifluoroethylene; polycarbonate; Polyesters such as polyethylene naphthalate and polyethylene terephthalate; nylons such as nylon 6, nylon 6,6 and nylon 4,10; polyimides; polysulfones;
Polyphenylene sulfide; Silicon resin such as polydiphenyl siloxane; Phenolic resin such as novolac; Epoxy resin; Polyurethane; Polystyrene; Butadiene-styrene copolymer; Cellulose, cellulose acetate, nitrocellulose, starch, calcium alginate, hydroxypropylmethylcellulose Examples include sugars, chitin, chitosan, sumac, polyamide such as gelatin, collagen, keratin, and copolymers of these polymer compounds. These may be composite materials, and may be filled with metal oxide particles, glass fibers, or the like, if desired, or may be used by blending with an organic compound material.

In the above embodiment, the biochemical analysis units 1 and 81 are the aluminum substrates 2 and 8.
Nylon 6 is filled in the large number of through holes formed in 2 to form a large number of dot-shaped absorptive regions 3 and 83 formed.
However, it is possible to form a large number of absorptive regions separated from each other by press-fitting the absorptive film formed of nylon 6 into a large number of through holes formed in the substrate. A porous plate having a large number of through holes may be adhered to one surface of the substrate to form a large number of absorptive regions separated from each other.

Further, in the above-mentioned embodiment, the biochemical analysis units 1 and 81 are the aluminum substrate 2,
Nylon 6 is filled in a large number of through holes formed in 82 to form a large number of dot-shaped absorptive regions 3 and 8 formed.
3, the absorptive region 3 of the biochemical analysis unit 1 and the absorptive region 83 of the biochemical analysis unit 81.
However, it is not always necessary to be formed of nylon 6, and instead of nylon 6, a carbon porous material such as activated carbon or nylons such as nylon 6,6 and nylon 4,10; nitrocellulose, cellulose acetate,
Cellulose derivatives such as cellulose acetate butyrate; collagen; alginic acid, calcium alginate, alginic acid /
Alginic acids such as polylysine polyion complex; polyolefins such as polyethylene and polypropylene; polyvinyl chloride; polyvinylidene chloride; polyfluoride such as polyvinylidene fluoride and polytetrafluoride; and copolymers or composites thereof,
The absorptive region 3 of the biochemical analysis unit 1 and the absorptive region 83 of the biochemical analysis unit 81 can also be formed, and further, a metal such as platinum, gold, iron, silver, nickel or aluminum; alumina, Metal oxides such as silica, titania, and zeolite; metal salts such as hydroxyapatite and calcium sulfate, inorganic porous materials such as composites thereof, or a bundle of a plurality of fibers, and the absorptive region 3 of the biochemical analysis unit 1 Also, the absorptive region 83 of the biochemical analysis unit 81 may be formed.

In the above embodiment, the substrates 2 and 82 of the biochemical analysis unit 1 and 81 are approximately 10,000.
Of substantially circular absorptive areas 3,83 having a size of about 0.01 square millimeters are formed according to a regular pattern with a density of about 5000 pieces / square centimeter. Is not necessarily formed into a substantially circular shape, and the absorptive regions 3 and 83 can be formed into an arbitrary shape, for example, a rectangular shape.

Furthermore, in the above-mentioned embodiment, the substrates 2 and 82 of the biochemical analysis unit 1 and 81 are approximately 1000.
The substantially circular absorptive regions 3, 83 having a size of about 0.01 square millimeter of 0 are formed according to a regular pattern with a density of about 5000 pieces / square centimeter, but the absorptive regions 3, The number and size of 83 can be arbitrarily selected according to the purpose, and preferably, the number of the adsorbent regions 3, 83 having a size of 50 or more and less than 5 mm 2 is 10 or more / cm 2 or more. Then, the biochemical analysis units 1 and 81 are formed.

In the above embodiment, the substrates 2 and 82 of the biochemical analysis unit 1 and 81 are approximately 10,000.
Of substantially circular absorptive areas 3,83 having a size of about 0.01 square millimeters are formed according to a regular pattern with a density of about 5000 pieces / square centimeter. It is not always necessary to form the biochemical analysis units 1 and 81 in a regular pattern.

Further, in the above-described embodiment, the biochemical analysis units 1 and 81 have a substrate 2 in which a large number of through holes formed in the aluminum substrates 2 and 82 are filled with nylon 6. Although a large number of dot-shaped absorptive regions 3 and 83 separated from each other are independently formed by 82, they are separated from each other by the substrates 2 and 82 in the biochemical analysis units 1 and 81. It is not always necessary to form the independent absorptive regions 3 and 83, and a unit for biochemical analysis is formed by an absorptive substrate formed of an absorptive material, and a specific binding substance is spotted on the absorptive substrate. It is also possible to form absorptive regions that are spaced apart from each other.

Further, in the above-mentioned embodiment, the stimulable phosphor sheets 10 and 90 are provided with nickel supports 11 and 91 in which a large number of substantially circular through holes are regularly formed.
A large number of through holes formed in the supports 11 and 91 are filled with stimulable phosphors, and a large number of stimulable phosphor layer regions 1 are formed.
2, 92 are formed in a dot shape, but instead of the through holes, a large number of substantially circular recesses are regularly formed in the supports 11 and 91, and photostimulable phosphors are provided in the recesses. By embedding, a large number of stimulable phosphor layer regions 12, 92 can be formed in a dot shape.

Further, in the above-mentioned embodiment, the stimulable phosphor sheets 10 and 90 are provided with nickel supports 11 and 91 in which a large number of substantially circular through holes are regularly formed. In the many through holes formed in 91,
A large number of stimulable phosphor layer regions 12, 92 are formed in a dot shape by being embedded with a stimulable phosphor, and are made of nickel in which a large number of substantially circular through holes are regularly formed. A large number of stimulable phosphor layer regions 12 and 92 can be formed in a dot shape by press-fitting a stimulable phosphor film containing a stimulable phosphor into the supports 11 and 91.

Further, in the above-mentioned embodiment, the stimulable phosphor sheets 10 and 90 are provided with nickel supports 11 and 91 in which a large number of substantially circular through holes are regularly formed. In the many through holes formed in 91,
A large number of photostimulable phosphor layer regions 12 and 92 are formed in a dot shape in which the photostimulable phosphor is embedded. The fluorescent phosphor layer region 12 can also be formed.

Further, in the above-mentioned embodiment, the stimulable phosphor sheets 10 and 90 are provided with nickel supports 11 and 91 in which a large number of substantially circular through holes are regularly formed.
A large number of through holes formed in the supports 11 and 91 are filled with stimulable phosphors, and a large number of stimulable phosphor layer regions 1 are formed.
Although 2, 92 are formed in a dot shape, it is not always necessary to form the support 11 with nickel,
It is also possible to form the supports 11 and 91 of the stimulable phosphor sheets 10 and 90 by using other materials. The supports 11 and 91 of the stimulable phosphor sheets 10 and 90 are preferably formed of a material having a property of attenuating radiation, but the material is not particularly limited and may be an inorganic compound material or an organic compound material. Both can be used, with metallic, ceramic or plastic materials being particularly preferred. Storage phosphor sheet 1
Examples of inorganic compound materials that can be preferably used to form the supports 11 and 91 of 0 and 90 and can attenuate radiation include gold, silver, copper, zinc, aluminum, titanium, tantalum, and chromium. Metals such as iron, nickel, cobalt, lead, tin and selenium; alloys such as brass, stainless steel and bronze; silicon materials such as silicon, amorphous silicon, glass, quartz, silicon carbide and silicon nitride; aluminum oxide, magnesium oxide, oxidation Metal oxides such as zirconium; tungsten carbide,
Inorganic salts such as calcium carbonate, calcium sulfate, hydroxyapatite and gallium arsenide can be mentioned.
These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic. In addition, the supports 11, 91 of the stimulable phosphor sheets 10, 90
A polymer compound is preferably used as an organic compound material that can be preferably used to form a compound and can attenuate radiation, and a preferable polymer compound is, for example, a polyolefin such as polyethylene or polypropylene; polymethylmethacrylate. Acrylic resin such as butyl acrylate / methyl methacrylate copolymer; polyacrylonitrile; polyvinyl chloride; polyvinylidene chloride; polyvinylidene fluoride; polytetrafluoroethylene; polychlorotrifluoroethylene; polycarbonate; polyethylene naphthalate or polyethylene terephthalate Polyester; nylon 6,
Nylon such as nylon 6,6 and nylon 4,10; polyimide; polysulfone; polyphenylene sulfide; silicon resin such as polydiphenylsiloxane; phenol resin such as novolac; epoxy resin; polyurethane; polystyrene; butadiene-styrene copolymer;
Polysaccharides such as cellulose, cellulose acetate, nitrocellulose, starch, calcium alginate, hydroxypropylmethylcellulose; chitin; chitosan; sumac;
Examples thereof include polyamides such as gelatin, collagen and keratin, and copolymers of these high molecular compounds. These may be composite materials, if necessary, it is possible to fill with metal oxide particles or glass fibers,
Also, an organic compound material can be blended and used.

Furthermore, in the above-mentioned embodiment, the absorptive regions 3 and 8 formed in the biochemical analysis unit 1 and 81.
Corresponding to 3, a substantially circular stimulable phosphor layer region 1 having a size of about 0.01 mm 2 of about 10000.
2, 92 are formed on the stimulable phosphor sheet 10, 90 according to a regular pattern with a density of about 5000 pieces / square centimeter, but the stimulable phosphor layer regions 12, 92 are formed into a substantially circular shape. It is not always necessary to form the photostimulable phosphor layer regions 12 and 92, and the photostimulable phosphor layer regions 12 and 92 can be formed in an arbitrary shape, for example, a rectangular shape.

Further, in the above-mentioned embodiment, the absorptive regions 3 and 83 formed in the biochemical analysis units 1 and 81.
Corresponding to a substantially circular stimulable phosphor layer region 12 having a size of about 0.01 square millimeters of about 10,000,
92 has a density of about 5000 pieces / square centimeter,
Accumulative phosphor sheet 1 according to a regular pattern
0 and 90, the stimulable phosphor layer region 1
The number and size of 2, 92 can be arbitrarily selected according to the purpose, and preferably 10 or more and the stimulable phosphor layer region 1 having a size of less than 5 mm 2.
2, 92 are formed on the stimulable phosphor sheet 10, 90 at a density of 10 pieces / square centimeter or more.

Furthermore, in the above-mentioned embodiment, the absorptive regions 3 and 8 formed in the biochemical analysis units 1 and 81.
Corresponding to 3, a substantially circular stimulable phosphor layer region 1 having a size of about 0.01 mm 2 of about 10000.
2, 92 are formed on the stimulable phosphor sheets 10, 90 in a regular pattern with a density of about 5000 pieces / square centimeter, and the absorptive region 3, of the biochemical analysis units 1, 81, It is not always necessary to form 83 in the biochemical analysis unit 1, 81 in a regular pattern, and therefore, the stimulable phosphor layer region 1
It is not always necessary to form 2, 92 in the stimulable phosphor sheet 10, 90 in a regular pattern, and the stimulable phosphor layer regions 12, 92 are provided in the biochemical analysis unit 1, 81. It is only necessary that the absorptive regions 3 and 83 thus formed are formed on the stimulable phosphor sheets 10 and 90 according to the same pattern.

In the above embodiment, the stimulable phosphor layer regions 12, 92 of the stimulable phosphor sheet 10, 90 are used.
Is formed in the same size as the absorptive regions 3 and 83 formed in the biochemical analysis units 1 and 81, but the stimulable phosphor layer regions 12 and 92 are formed in the biochemical analysis unit 1,
It is not always necessary to form the same size as the absorptive regions 3 and 83 formed in 81, and preferably the absorptive regions 3 and 8 formed in the biochemical analysis unit 1 and 81.
It is formed to have a size of 3 or more.

Furthermore, in the above-mentioned embodiment, the stimulable phosphor sheet 10, 90 is provided with nickel supports 11, 91 in which a large number of substantially circular through holes are regularly formed. In the many through holes formed in 91,
A large number of stimulable phosphor layer regions 12 and 92 are formed in a dot shape by embedding the stimulable phosphor, but the stimulable phosphor sheets 10 and 90 are separated from each other in a dot shape. It is not always necessary to provide the stimulable phosphor layer region 12 formed in 1., and the stimulable phosphor layer can be uniformly formed on the surface of the support.

[0285] In the above embodiment, the hybridizing solution 9 containing the substance of biological origin labeled with a radioactive labeling substance and the substance of biological origin labeled with a fluorescent substance such as a fluorescent dye is prepared, and the adsorptive region is prepared. Although it is hybridized with the specific binding substance dropped on 3, it is not always necessary that the biological substance is labeled with a radioactive substance and a fluorescent substance such as a fluorescent dye. It should have been done.

Furthermore, in the above-mentioned embodiment, the substance of biological origin labeled with a radioactive labeling substance and a fluorescent substance such as a fluorescent dye is hybridized with the specific binding substance. It is not always necessary to hybridize with a specific binding substance,
Instead of using a substance derived from a living body for hybridization,
It is also possible to specifically bind to a specific binding substance by an antigen-antibody reaction or a reaction such as a receptor / ligand.

Further, in the embodiment shown in FIGS. 1 to 4, the substrate 2 of the biochemical analysis unit 1 and the support 11 of the stimulable phosphor sheet 10 have seven circular through holes for matching determination. 4 and 13 are formed so that the substrate 2 of the biochemical analysis unit 1 and the support 11 of the stimulable phosphor sheet 10 are circular through holes 4 for matching determination of 2 or more and less than 7. 13 may be formed, or eight or more circular matching determination through holes 4 and 13 may be formed.

Further, in the embodiment shown in FIGS. 1 to 4, the substrate 2 of the biochemical analysis unit 1 is provided with 4 units.
Although four circular matching determination through-holes 4 are formed, it is not always necessary to form four circular matching determination through-holes 4 in the substrate 2 of the biochemical analysis unit 1, and seven matching matching through-holes 4 are formed. When the determination through holes 4 can be formed, the number of matching determination through holes 4 may be 7 or less.

Further, in the embodiment shown in FIGS. 1 to 4, the support body 11 of the stimulable phosphor sheet 10 has three circular through holes 13 for matching determination. On the support 11 of the fluorescent phosphor sheet 10,
It is not always necessary to form the three circular matching determination through holes 13, and when the seven matching determination through holes 13 can be formed, the number of the matching determination through holes 13 may be 7 or less. .

Further, in the embodiment shown in FIG. 1 to FIG. 4, the substrate 2 of the biochemical analysis unit 1 has four substrates.
Two circular matching determination through holes 4 are formed, and three circular matching determination through holes 13 are formed in the support 11 of the stimulable phosphor sheet 10. It is not always necessary that the determination through hole 13 has a circular shape, and further, the matching determination through hole 4 and the matching determination through hole 13 may have different shapes.

In the embodiment shown in FIGS. 21 to 22, the substrate 82 of the biochemical analysis unit 81 is used.
The support 91 of the stimulable phosphor sheet 90 is configured so that seven semi-circular matching determination notches 84 and 93 can be formed. However, the substrate 82 and the stimulable phosphor of the biochemical analysis unit 81 are formed. The support 91 of the seat 90,
2 or more and less than 7 circular notch for matching judgment 8
4 and 93 may be formed, or 8 or more circular matching determination notches 84 and 93 may be formed.

Further, in the embodiment shown in FIGS. 21 to 22, the substrate 8 of the biochemical analysis unit 81 is used.
Although two semicircular notches 84 for matching determination are formed on the substrate 2, the substrate 8 of the biochemical analysis unit 81 is formed.
It is not always necessary to form four circular matching determination notches 84 in 2, and when seven matching determination notches 84 can be formed, the number of matching determination notches 84 is 7 or less. Yes

Further, in the embodiment shown in FIGS. 21 to 22, the support 91 of the stimulable phosphor sheet 90 is used.
Four semi-circular notches 93 for matching determination are formed in the support member 91 of the stimulable phosphor sheet 90.
In addition, it is not always necessary to form four circular matching determination cutouts 93, and when seven matching determination cutouts 93 can be formed, the number of matching determination cutouts 93 must be 7 or less. Good.

Further, in the embodiment shown in FIGS. 21 to 22, the substrate 8 of the biochemical analysis unit 81 is used.
2, four semi-circular notches 84 for matching determination are formed on the support 91 of the stimulable phosphor sheet 90.
Although two semicircular matching determination cutouts 93 are formed, it is not always necessary that the matching determination cutout 84 and the matching determination cutout 93 have a semicircular shape. The cutout 84 and the matching determination cutout 93 may have different shapes.

Further, in the embodiment shown in FIGS. 1 to 4, four circular matching determination through-holes 4 are formed in the substrate 2 of the biochemical analysis unit 1 and the biochemical analysis unit 1 is used for biochemical analysis. In the embodiment shown in FIGS. 21 to 22, three circular through holes 13 for matching determination are formed in the support 11 of the stimulable phosphor sheet 10 that is allowed to be used with the unit 1. Is a stimulable phosphor in which four semicircular matching determination notches 84 are formed on the substrate 82 of the biochemical analysis unit 81 and which is allowed to be used with the biochemical analysis unit 81. The support 91 of the sheet 90 has four semicircular notches 9 for matching determination.
3 is formed, but the biochemical analysis units 1 and 81 and the stimulable phosphor sheets 10 and 90, which are allowed to be used together, respectively, at least partially,
The complementary shape is sufficient, and the through holes 4 for matching determination and the notches 84 for matching determination are formed in the substrates 2 and 82 of the biochemical analysis units 1 and 81, and the stimulable phosphor sheet 10 is formed. , 90 of the supports 11 and 91, the through hole 13 for matching determination and the notch 93 for matching determination.
Need not be formed, and for example, the substrate 2 of the corresponding biochemical analysis unit 1, 81,
82 and the surfaces of the supports 11, 91 of the stimulable phosphor sheets 10, 90 are formed with complementary irregularities,
Other than the corresponding biochemical analysis units 1 and 81 and the stimulable phosphor sheets 10 and 90, the corresponding biochemical analysis units 1 and 81 and the stimulable phosphor are placed in close contact with each other so that they cannot be exposed. The sheets 10, 90 can also be formed.

[0296]

INDUSTRIAL APPLICABILITY According to the present invention, a biochemical substance, which is collected from a person whose gene is abnormally expressed and is labeled with a radiolabeled substance, is selectively selected by, for example, hybridization. The specific binding substance contained in the spot-shaped region formed in the above, specifically binds, and the biochemical analysis unit and the stimulable phosphor layer-formed stimulable phosphor sheet are superposed. After the exposure with the radioactive labeling substance selectively contained in the spot-shaped region, the photostimulable phosphor layer exposed with the radioactive labeling substance is scanned with excitation light to excite the photostimulable phosphor. , Photoelectrically detecting the stimulable light emitted from the stimulable phosphor to generate data for biochemical analysis, and collect from a healthy person, and a substance of biological origin labeled with a radiolabeled substance. , A specific binding substance contained in the spot-like region formed in the biochemical analysis unit is selectively bound by hybridization, and the biochemical analysis unit and the stimulable phosphor The stimulable phosphor layer exposed by the radio-labeled substance is exposed after being overlapped with the stimulable phosphor sheet on which the layer is formed and exposed by the radio-labeled substance selectively contained in the spot-shaped region. Scanning with excitation light to excite the stimulable phosphor, photoelectrically detecting the stimulable light emitted from the stimulable phosphor to generate biochemical analysis data, and obtain the obtained biochemistry. A biochemical analysis kit including a biochemical analysis unit and a stimulable phosphor sheet and a stimulable phosphor that can improve the inspection accuracy of gene expression abnormalities by comparing analysis data and performing a test It is possible to provide over bets exposure apparatus.

[Brief description of drawings]

FIG. 1 is a schematic perspective view of a biochemical analysis unit according to a preferred embodiment of the present invention.

FIG. 2 is a schematic front view of a spotting device.

FIG. 3 is a schematic vertical sectional view of a hybridization container.

FIG. 4 is a schematic perspective view of a stimulable phosphor sheet according to a preferred embodiment of the present invention.

FIG. 5 is a schematic perspective view of an exposure apparatus for a stimulable phosphor sheet according to a preferred embodiment of the present invention.

FIG. 6 is a schematic partial cross-sectional view of a stimulable phosphor sheet exposure apparatus according to a preferred embodiment of the present invention.

FIG. 7 is a schematic plan view for explaining a relative positional relationship between a through hole formed in a substrate of a biochemical analysis unit and a through hole formed in a support of a stimulable phosphor sheet. It is a figure.

FIG. 8 shows a large number of dot-shaped stimulable phosphors formed on a stimulable phosphor sheet by a radiolabel substance contained in a large number of absorptive regions formed in a biochemical analysis unit. FIG. 7 is a schematic cross-sectional view showing a method of exposing a layer region.

FIG. 9 shows a biochemical analysis by reading the radiation data recorded in the stimulable phosphor layer area of the stimulable phosphor sheet and the fluorescence data recorded in the absorptive area of the biochemical analysis unit. 3 is a schematic perspective view of a scanner for generating usage data. FIG.

FIG. 10 shows a biochemical analysis by reading the radiation data recorded in the stimulable phosphor layer area of the stimulable phosphor sheet and the fluorescence data recorded in the absorptive area of the biochemical analysis unit. FIG. 3 is a schematic perspective view showing details of the vicinity of a photomultiplier of a scanner for generating usage data.

11 is a schematic cross-sectional view taken along the line AA of FIG.

12 is a schematic cross-sectional view taken along the line BB of FIG.

FIG. 13 is a schematic cross-sectional view taken along the line CC of FIG.

FIG. 14 is a schematic cross-sectional view taken along the line DD of FIG.

FIG. 15 is a schematic plan view of a scanning mechanism of an optical head.

FIG. 16 is a block diagram showing a control system, an input system, a drive system and a detection system of the scanner according to the preferred embodiment of the present invention.

FIG. 17 is a schematic perspective view of an exposure apparatus for a stimulable phosphor sheet according to another preferred embodiment of the present invention.

FIG. 18 is a block diagram showing a control system, a detection system and a display system of an exposure apparatus for a stimulable phosphor sheet according to another preferred embodiment of the present invention.

FIG. 19 is a schematic partial cross-sectional view showing a mechanism for locking the lid member of the stimulable phosphor sheet exposure apparatus according to another embodiment of the present invention to the casing.

FIG. 20 is a block diagram showing a control system, a detection system, a drive system and a display system of an exposure apparatus for a stimulable phosphor sheet according to another embodiment of the present invention.

FIG. 21 is a schematic perspective view of a biochemical analysis unit according to another preferred embodiment of the present invention.

FIG. 22 is a schematic perspective view of a stimulable phosphor sheet according to another preferred embodiment of the present invention.

FIG. 23 is a schematic perspective view of a stimulable phosphor sheet exposure apparatus according to another preferred embodiment of the present invention.

FIG. 24 shows the relative positional relationship between the matching determination notch formed on the substrate of the biochemical analysis unit and the matching determination notch formed on the support of the stimulable phosphor sheet. It is a schematic plan view for explaining.

[Explanation of symbols]

1 Biochemical analysis unit 2 substrates 3 Adsorbable area 4 through holes 5 Through hole for positioning 6 injectors 7 CCD camera 8 Hybridization container 9 Hybridization solution 10 Storage phosphor sheet 11 Support 12 Photostimulable phosphor layer area 13 through holes 14 Through hole for positioning 15 casing 15a Lid member shaft 16 Support plate 17 Lid member 18 Positioning pin 19 Transparent glass plate 20 fluorescent light 21 First laser excitation light source 22 Second laser excitation light source 23 Third Laser Excitation Light Source 24 laser light 25 Collimator lens 26 mirror 27 First Dichroic Mirror 28 Second dichroic mirror 29 mirror 30 collimator lens 31 Collimator lens 32 mirror 33 holes for the perforated mirror 34 perforated mirror 35 Optical head 36 mirror 37 Aspherical lens 38 concave mirror 40 stages 41 glass plate 45 Fluorescence or stimulated emission 48 filter units 50 Photomultiplier 51a, 51b, 51c, 51d filter member 52a, 52b, 52c, 52d filters 53 A / D converter 54 Photon Counter 60 substrates 61 Sub-scanning pulse motor 62 a pair of rails 63 Movable substrate 64 rod 65 Main scanning stepping motor 66 endless belt 67 Linear encoder 68 Linear encoder slit 70 Control unit 71 keyboard 72 Filter unit motor 73 sensor 74 Display panel 75 CPU 76 Alarm means 77 Hook member 77a Hook member shaft 78 Compression spring 79 Engagement groove 80 solenoid 81 Biochemical analysis unit 82 substrate 83 Adsorbent area 84 Notch for matching judgment 85 Positioning through hole 90 Storage phosphor sheet 91 Support 92 Photostimulable phosphor layer region 93 Notch for matching judgment 94 Positioning through hole 105 casing 105a Lid member shaft 106 support plate 107 lid member 108 Positioning pin 109 biasing member

Claims (44)

[Claims] 1. A biochemical analysis unit having an adsorption layer formed of an adsorptive material, and a stimulable phosphor sheet having a stimulable phosphor layer formed thereon, the biochemical analysis unit and the biochemical analysis unit. A biochemical analysis kit, wherein each of the stimulable phosphor sheets has a complementary shape to at least a part thereof. 2. The biochemical analysis unit and the stimulable phosphor sheet are configured to form a plurality of through holes, and are complementary to each other in the biochemical analysis unit and the stimulable phosphor sheet. The biochemical analysis kit according to claim 1, wherein at least one through hole is formed so as to have a shape. 3. The biochemical analysis unit and the stimulable phosphor sheet are configured to form a plurality of notches, and are complementary to each other in the biochemical analysis unit and the stimulable phosphor sheet. The biochemical analysis kit according to claim 1, wherein at least one notch is formed so as to have a shape. 4. A specific binding substance having a known structure or characteristic is dropped on the adsorption layer of the biochemical analysis unit to form a plurality of spot-shaped regions separated from each other. The kit for biochemical analysis according to any one of claims 1 to 3. 5. The biochemical analysis unit comprises a substrate having a plurality of holes formed therein, and the adsorption layer is constituted by an absorptive region formed in the plurality of holes. The biochemical analysis kit according to any one of claims 1 to 3. 6. The absorptive region of the biochemical analysis unit is formed by filling an absorptive material in the plurality of holes of the substrate. Biochemical analysis kit. 7. The absorptive region of the biochemical analysis unit is formed by press-fitting an absorptive film containing an absorptive material into the plurality of holes of the substrate. Item 8. A biochemical analysis kit according to item 6. 8. The biochemical analysis kit according to claim 1, wherein the adsorbent material contains a porous material. 9. The biochemical analysis kit according to claim 8, wherein the adsorptive material contains a carbon porous material or a porous material capable of forming a membrane filter. 10. The biochemical analysis kit according to claim 1, wherein the adsorptive material contains a fibrous material. 11. The biochemical analysis kit according to claim 1, wherein the absorptive region is regularly formed. 12. The biochemical analysis kit according to claim 7, wherein 10 or more absorptive regions are formed on the substrate of the biochemical analysis unit. . 13. The biochemical analysis kit according to claim 12, wherein 1000 or more of the absorptive regions are formed on the substrate of the biochemical analysis unit. 14. The biochemical analysis kit according to claim 13, wherein 10,000 or more absorptive regions are formed on the substrate of the biochemical analysis unit. 15. The absorptive region has a size of less than 5 square millimeters.
15. The kit for biochemical analysis according to any one of 1 to 14. 16. The absorptive region has a size of less than one square millimeter.
The kit for biochemical analysis according to item 5. 17. The biochemical analysis kit according to claim 16, wherein the adsorptive region has a size of less than 0.1 mm 2. 18. The substrate according to claim 5, wherein the absorptive regions are formed on the substrate of the biochemical analysis unit at a density of 10 or more per square centimeter. Biochemical analysis kit. 19. The absorptive region is formed on the substrate of the biochemical analysis unit at a density of 1000 pieces / cm 2 or more.
The kit for biochemical analysis according to. 20. The biochemical analysis kit according to claim 19, wherein the absorptive regions are formed on the substrate of the biochemical analysis unit at a density of 10,000 or more per cm 2. . 21. The substrate of the biochemical analysis unit,
The biochemical analysis kit according to any one of claims 5 to 20, which has a property of attenuating radiation. 22. When the radiation of the substrate of the biochemical analysis unit is transmitted through the substrate by a distance equal to the distance between the adjacent absorptive regions, the energy of the radiation is reduced to 1/5. 22. The biochemical analysis kit according to claim 21, which has the following attenuation property. 23. When the radiation of the substrate of the biochemical analysis unit is equal to the distance between the adsorbing regions adjacent to each other, the energy of the radiation is 1/10 or less. 23. The kit for biochemical analysis according to claim 22, which has a property of being attenuated to. 24. When the radiation of the substrate of the biochemical analysis unit is equal to the distance between the adsorbing regions adjacent to each other, the radiation energy is 1/100 or less. 24. The biochemical analysis kit according to claim 23, which has the property of being attenuated. 25. The substrate of the biochemical analysis unit is formed of a material selected from the group consisting of a metal material, a ceramic material, and a plastic material. The kit for biochemical analysis according to. 26. The specific binding substance having a known structure or characteristic is dropped onto the adsorptive region of the biochemical analysis unit. Biochemical analysis kit. 27. The stimulable phosphor layer region in which the stimulable phosphor sheet is provided with a support having a plurality of holes formed therein, and the stimulable phosphor layer is formed in the plurality of holes. The photostimulable phosphor layer region is formed according to the same pattern as the absorptive region of the biochemical analysis unit.
6. The biochemical analysis kit according to any one of 6 above. 28. The photostimulable phosphor layer region of the stimulable phosphor sheet is formed by filling a photostimulable phosphor into the plurality of holes of the support. The kit for biochemical analysis according to claim 27. 29. A photostimulable phosphor film containing a photostimulable phosphor is press-fitted into the holes of the support in the photostimulable phosphor layer region of the stimulable phosphor sheet. 29. The kit for biochemical analysis according to claim 28, which is formed. 30. The stimulable phosphor sheet comprises a support, and the stimulable phosphor layer is formed on the surface of the support in the same pattern as the absorptive region of the biochemical analysis unit. 27. The biochemical analysis kit according to any one of claims 5 to 26, characterized in that it is constituted by a plurality of stimulable phosphor layer regions formed. 31. The biochemical analysis kit according to claim 27, wherein the stimulable phosphor layer region has a size of less than 5 mm 2. 32. The biochemical analysis kit according to claim 31, wherein the stimulable phosphor layer region has a size of less than 1 mm 2. 33. The biochemical analysis kit according to claim 32, wherein the photostimulable phosphor layer region has a size of less than 0.1 mm 2. 34. The biochemical analysis kit according to claim 27, wherein the support of the stimulable phosphor sheet has a property of attenuating radiation. . 35. When radiation is transmitted through the support by a distance equal to the distance between the adjacent photostimulable phosphor layer regions, the support of the stimulable phosphor sheet comprises:
The biochemical analysis kit according to claim 34, which has a property of attenuating the energy of radiation to 1/5 or less. 36. When the support of the stimulable phosphor sheet is such that radiation penetrates through the support by a distance equal to the distance between adjacent photostimulable phosphor layer regions,
The biochemical analysis kit according to claim 35, which has a property of attenuating the energy of radiation to 1/10 or less. 37. When radiation is transmitted through the support by a distance equal to the distance between the adjacent photostimulable phosphor layer regions, the support of the stimulable phosphor sheet is:
The biochemical analysis kit according to claim 36, which has a property of attenuating the energy of radiation to 1/100 or less. 38. The support of the stimulable phosphor sheet is formed of a material selected from the group consisting of metal materials, ceramic materials and plastic materials. The biochemical analysis kit according to item 1. 39. A biochemical analysis unit including a casing and a lid member formed of a material that attenuates radiation, and an adsorption layer formed of an adsorbent material; and a stimulable phosphor layer. An exposure apparatus for a stimulable phosphor sheet, characterized in that each of the stimulable phosphor sheet is provided with a detection means for detecting whether or not the stimulable phosphor sheet has a shape complementary to each other. 40. The detection means detects light transmitted through at least one through hole formed in the biochemical analysis unit and the stimulable phosphor sheet so as to have complementary shapes to each other. 40. The exposure apparatus for a stimulable phosphor sheet according to claim 39, which is configured to: 41. The detection means is configured to detect at least one notch formed in each of the biochemical analysis unit and the stimulable phosphor sheet so as to have a shape complementary to each other. 40. The stimulable phosphor sheet exposure apparatus according to claim 39, wherein 42. Further, when it is determined that the biochemical analysis unit and the stimulable phosphor sheet do not have shapes complementary to each other, the biochemical analysis unit and the stimulable fluorescence sheet. 42. An apparatus for exposing a stimulable phosphor sheet according to any one of claims 39 to 41, characterized in that the body sheet is provided with a display means for indicating that both cannot be used. 43. Further, an alarm means is provided for generating an alarm sound when it is determined that the biochemical analysis unit and the stimulable phosphor sheet do not have complementary shapes. 39. The method according to claim 39,
43. An exposure device for a stimulable phosphor sheet according to any one of 3 to 42. 44. The lid member cannot be closed when it is determined that the biochemical analysis unit and the stimulable phosphor sheet do not have complementary shapes. 44. The exposure apparatus for a stimulable phosphor sheet according to claim 39, further comprising locking means for locking.