JP2002541823A5 - - Google Patents
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- Publication number
- JP2002541823A5 JP2002541823A5 JP2000611727A JP2000611727A JP2002541823A5 JP 2002541823 A5 JP2002541823 A5 JP 2002541823A5 JP 2000611727 A JP2000611727 A JP 2000611727A JP 2000611727 A JP2000611727 A JP 2000611727A JP 2002541823 A5 JP2002541823 A5 JP 2002541823A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- oligonucleotide
- amplification
- reporter
- target nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000003321 amplification Effects 0.000 description 67
- 238000003199 nucleic acid amplification method Methods 0.000 description 67
- 150000007523 nucleic acids Chemical class 0.000 description 55
- 238000000034 method Methods 0.000 description 54
- 108091034117 Oligonucleotide Proteins 0.000 description 53
- 108020004707 nucleic acids Proteins 0.000 description 47
- 102000039446 nucleic acids Human genes 0.000 description 47
- 230000000295 complement effect Effects 0.000 description 26
- 239000003381 stabilizer Substances 0.000 description 26
- 230000000087 stabilizing effect Effects 0.000 description 16
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 12
- 108091028043 Nucleic acid sequence Proteins 0.000 description 8
- 230000000368 destabilizing effect Effects 0.000 description 7
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 6
- 230000002452 interceptive effect Effects 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- 230000014759 maintenance of location Effects 0.000 description 4
- 108091093088 Amplicon Proteins 0.000 description 3
- 229960002685 biotin Drugs 0.000 description 3
- 235000020958 biotin Nutrition 0.000 description 3
- 239000011616 biotin Substances 0.000 description 3
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical class O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 3
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical class [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 3
- 238000005096 rolling process Methods 0.000 description 3
- 238000000137 annealing Methods 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/290,129 | 1999-04-12 | ||
| US09/290,129 US6294884B1 (en) | 1998-04-21 | 1999-04-12 | Vertical deflection circuit and color picture tube apparatus |
| PCT/US2000/008432 WO2000061805A1 (en) | 1999-04-12 | 2000-03-28 | Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2002541823A JP2002541823A (ja) | 2002-12-10 |
| JP2002541823A5 true JP2002541823A5 (https=) | 2007-05-24 |
Family
ID=23114655
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000611727A Pending JP2002541823A (ja) | 1999-04-12 | 2000-03-28 | バイオエレクトニック・マイクロチップを用いる単一核酸多型の決定方法 |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP1173611A4 (https=) |
| JP (1) | JP2002541823A (https=) |
| AU (1) | AU769929B2 (https=) |
| MX (1) | MXPA01010381A (https=) |
| NO (1) | NO20014932L (https=) |
| WO (1) | WO2000061805A1 (https=) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6379897B1 (en) | 2000-11-09 | 2002-04-30 | Nanogen, Inc. | Methods for gene expression monitoring on electronic microarrays |
| US6596487B2 (en) | 2000-03-10 | 2003-07-22 | Ana-Gen Technologies, Inc. | Mutation detection using denaturing gradients |
| KR100437625B1 (ko) * | 2001-09-17 | 2004-06-30 | 주식회사 마이진 | Ｚｉｐ-Ｃｏｄｅ 올리고염기 칩을 이용한 단일염기 다형성검사 방법과 검사 키트 |
| GB0205455D0 (en) | 2002-03-07 | 2002-04-24 | Molecular Sensing Plc | Nucleic acid probes, their synthesis and use |
| DE102004023439B4 (de) * | 2004-05-12 | 2006-03-16 | Eppendorf Ag | Nachweis von RNA über Micro-Arrays |
| JP2006284262A (ja) | 2005-03-31 | 2006-10-19 | Sony Corp | 正弦波電圧を重畳印加する物質間相互作用検出装置及び方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5849486A (en) * | 1993-11-01 | 1998-12-15 | Nanogen, Inc. | Methods for hybridization analysis utilizing electrically controlled hybridization |
| US6017696A (en) * | 1993-11-01 | 2000-01-25 | Nanogen, Inc. | Methods for electronic stringency control for molecular biological analysis and diagnostics |
| US5605662A (en) * | 1993-11-01 | 1997-02-25 | Nanogen, Inc. | Active programmable electronic devices for molecular biological analysis and diagnostics |
| US5632957A (en) * | 1993-11-01 | 1997-05-27 | Nanogen | Molecular biological diagnostic systems including electrodes |
| US5795714A (en) * | 1992-11-06 | 1998-08-18 | Trustees Of Boston University | Method for replicating an array of nucleic acid probes |
| US6207373B1 (en) * | 1998-02-25 | 2001-03-27 | Nanogen, Inc. | Methods for determining nature of repeat units in DNA |
| US5770365A (en) * | 1995-08-25 | 1998-06-23 | Tm Technologies, Inc. | Nucleic acid capture moieties |
-
2000
- 2000-03-28 AU AU39305/00A patent/AU769929B2/en not_active Ceased
- 2000-03-28 MX MXPA01010381A patent/MXPA01010381A/es not_active Application Discontinuation
- 2000-03-28 JP JP2000611727A patent/JP2002541823A/ja active Pending
- 2000-03-28 EP EP00918506A patent/EP1173611A4/en not_active Withdrawn
- 2000-03-28 WO PCT/US2000/008432 patent/WO2000061805A1/en not_active Ceased
-
2001
- 2001-10-10 NO NO20014932A patent/NO20014932L/no not_active Application Discontinuation
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