JP2002526785A - 疎水性防壁によって規定された反応区域を有するスライド - Google Patents
疎水性防壁によって規定された反応区域を有するスライドInfo
- Publication number
- JP2002526785A JP2002526785A JP2000564087A JP2000564087A JP2002526785A JP 2002526785 A JP2002526785 A JP 2002526785A JP 2000564087 A JP2000564087 A JP 2000564087A JP 2000564087 A JP2000564087 A JP 2000564087A JP 2002526785 A JP2002526785 A JP 2002526785A
- Authority
- JP
- Japan
- Prior art keywords
- slide
- reaction zone
- hydrophobic
- substrate
- barrier
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 79
- 230000004888 barrier function Effects 0.000 title claims abstract description 36
- 230000002209 hydrophobic effect Effects 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 claims abstract description 34
- 230000008569 process Effects 0.000 claims abstract description 9
- 238000012545 processing Methods 0.000 claims description 21
- 239000000758 substrate Substances 0.000 claims description 21
- 239000000126 substance Substances 0.000 claims description 13
- 239000004809 Teflon Substances 0.000 claims description 7
- 229920006362 Teflon® Polymers 0.000 claims description 7
- 239000007864 aqueous solution Substances 0.000 claims description 6
- 230000002708 enhancing effect Effects 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 5
- 230000008878 coupling Effects 0.000 claims 3
- 238000010168 coupling process Methods 0.000 claims 3
- 238000005859 coupling reaction Methods 0.000 claims 3
- 210000004027 cell Anatomy 0.000 description 18
- 239000003153 chemical reaction reagent Substances 0.000 description 13
- 239000011521 glass Substances 0.000 description 13
- 239000000976 ink Substances 0.000 description 10
- 239000000463 material Substances 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 9
- 239000005361 soda-lime glass Substances 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- AKEJUJNQAAGONA-UHFFFAOYSA-N sulfur trioxide Chemical compound O=S(=O)=O AKEJUJNQAAGONA-UHFFFAOYSA-N 0.000 description 6
- 238000012744 immunostaining Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 230000021164 cell adhesion Effects 0.000 description 4
- 239000000975 dye Substances 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- BRPQOXSCLDDYGP-UHFFFAOYSA-N calcium oxide Chemical compound [O-2].[Ca+2] BRPQOXSCLDDYGP-UHFFFAOYSA-N 0.000 description 3
- 239000000292 calcium oxide Substances 0.000 description 3
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 238000002405 diagnostic procedure Methods 0.000 description 3
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N iron oxide Inorganic materials [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 3
- 239000000395 magnesium oxide Substances 0.000 description 3
- CPLXHLVBOLITMK-UHFFFAOYSA-N magnesium oxide Inorganic materials [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 description 3
- AXZKOIWUVFPNLO-UHFFFAOYSA-N magnesium;oxygen(2-) Chemical compound [O-2].[Mg+2] AXZKOIWUVFPNLO-UHFFFAOYSA-N 0.000 description 3
- TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 description 3
- NDLPOXTZKUMGOV-UHFFFAOYSA-N oxo(oxoferriooxy)iron hydrate Chemical compound O.O=[Fe]O[Fe]=O NDLPOXTZKUMGOV-UHFFFAOYSA-N 0.000 description 3
- 229920000729 poly(L-lysine) polymer Polymers 0.000 description 3
- CHWRSCGUEQEHOH-UHFFFAOYSA-N potassium oxide Chemical compound [O-2].[K+].[K+] CHWRSCGUEQEHOH-UHFFFAOYSA-N 0.000 description 3
- 229910001950 potassium oxide Inorganic materials 0.000 description 3
- 239000000377 silicon dioxide Substances 0.000 description 3
- 235000012239 silicon dioxide Nutrition 0.000 description 3
- KKCBUQHMOMHUOY-UHFFFAOYSA-N sodium oxide Chemical compound [O-2].[Na+].[Na+] KKCBUQHMOMHUOY-UHFFFAOYSA-N 0.000 description 3
- 229910001948 sodium oxide Inorganic materials 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 230000002380 cytological effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 238000013508 migration Methods 0.000 description 2
- 230000005012 migration Effects 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000002985 plastic film Substances 0.000 description 2
- 229920006255 plastic film Polymers 0.000 description 2
- 230000003252 repetitive effect Effects 0.000 description 2
- ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- 241000611421 Elia Species 0.000 description 1
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 1
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 1
- 101710093543 Probable non-specific lipid-transfer protein Proteins 0.000 description 1
- 150000008044 alkali metal hydroxides Chemical class 0.000 description 1
- 229910001860 alkaline earth metal hydroxide Inorganic materials 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 230000004956 cell adhesive effect Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 239000002274 desiccant Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000006862 enzymatic digestion Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000005338 frosted glass Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229920000724 poly(L-arginine) polymer Polymers 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 108010011110 polyarginine Proteins 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 238000000163 radioactive labelling Methods 0.000 description 1
- 230000002940 repellent Effects 0.000 description 1
- 239000005871 repellent Substances 0.000 description 1
- 238000007788 roughening Methods 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- -1 sodium hydroxide Chemical class 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 229940063673 spermidine Drugs 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Microscoopes, Condenser (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13066198A | 1998-08-07 | 1998-08-07 | |
| US09/130,661 | 1998-08-07 | ||
| PCT/US1999/013382 WO2000008511A1 (en) | 1998-08-07 | 1999-06-14 | Slides with reaction zones defined by hydrophobic barriers |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2002526785A true JP2002526785A (ja) | 2002-08-20 |
| JP2002526785A5 JP2002526785A5 (https=) | 2006-08-10 |
Family
ID=22445741
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000564087A Pending JP2002526785A (ja) | 1998-08-07 | 1999-06-14 | 疎水性防壁によって規定された反応区域を有するスライド |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP1101143A1 (https=) |
| JP (1) | JP2002526785A (https=) |
| WO (1) | WO2000008511A1 (https=) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007506933A (ja) * | 2003-08-01 | 2007-03-22 | キャピタルバイオ コーポレーション | 制御可能な反応体積を有するマイクロアレイデバイス |
| JP2015505966A (ja) * | 2011-12-09 | 2015-02-26 | ザ スクリップス リサーチ インスティテュート | 循環腫瘍細胞を特定するための装置、システム、及び方法 |
| JP2015176136A (ja) * | 2014-03-18 | 2015-10-05 | 株式会社内田洋行 | 顕微鏡観察試料用具 |
| JP7790807B1 (ja) * | 2024-07-02 | 2025-12-23 | 株式会社GramEye | スライドガラスおよび染色方法 |
| WO2026009852A1 (ja) * | 2024-07-02 | 2026-01-08 | 株式会社GramEye | スライドガラスおよび染色方法 |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102009015596A1 (de) | 2009-03-30 | 2010-10-21 | Dcs Innovative Diagnostik-Systeme Dr. Christian Sartori Gmbh & Co. Kg | Verfahren und Vorrichtung zur Behandlung von trägerfixiertem Material |
| WO2020092550A1 (en) * | 2018-11-01 | 2020-05-07 | Leavitt Medical, Inc. | Pathological slides and related systems and methods |
| US20240077712A1 (en) * | 2019-10-16 | 2024-03-07 | Hitachi High-Tech Corporation | Microscope Slide and Method for Selecting the Same |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3515160A1 (de) * | 1985-04-26 | 1986-11-06 | Klaus J. Dr.med. 7800 Freiburg Bross | Verfahren zur herstellung von objekttraegern mit abgegrenzten reaktionsfeldern und die dabei erhaltenen objekttraeger |
-
1999
- 1999-06-14 EP EP99928640A patent/EP1101143A1/en not_active Withdrawn
- 1999-06-14 JP JP2000564087A patent/JP2002526785A/ja active Pending
- 1999-06-14 WO PCT/US1999/013382 patent/WO2000008511A1/en not_active Ceased
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007506933A (ja) * | 2003-08-01 | 2007-03-22 | キャピタルバイオ コーポレーション | 制御可能な反応体積を有するマイクロアレイデバイス |
| JP2015505966A (ja) * | 2011-12-09 | 2015-02-26 | ザ スクリップス リサーチ インスティテュート | 循環腫瘍細胞を特定するための装置、システム、及び方法 |
| JP2015176136A (ja) * | 2014-03-18 | 2015-10-05 | 株式会社内田洋行 | 顕微鏡観察試料用具 |
| JP7790807B1 (ja) * | 2024-07-02 | 2025-12-23 | 株式会社GramEye | スライドガラスおよび染色方法 |
| WO2026009852A1 (ja) * | 2024-07-02 | 2026-01-08 | 株式会社GramEye | スライドガラスおよび染色方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP1101143A1 (en) | 2001-05-23 |
| WO2000008511A1 (en) | 2000-02-17 |
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Legal Events
| Date | Code | Title | Description |
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