JP2002267655A - Plant of genus euonymus family salacia and/or method of judging quality in extract from the plant - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a plant of genus euonymus family salacia that is useful for preventing or treating diabetes, hyperlipemia, and fatness, and/or their extract, a formulation containing them, or a method of judging the quality of food. SOLUTION: The method of judging the quality of the plant of genus euonymus family salacia and/or the extract from the plant has a quality judgment standard where mangiphelin having the following structure expression is contained or not.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to Salacia which is useful for preventing or treating diabetes, hyperlipidemia and obesity.
The present invention relates to a method for determining the quality of plants of the genus and / or extracts therefrom, formulations or food products containing them.

[0002]

2. Description of the Related Art A plant belonging to the genus Salachia (Salixia)
Reticulata (Salacia reticulata) is a vine that grows mainly in warm and humid regions such as Sri Lanka and India.It is used locally to treat inflammation, menses, ulcers, liver protection and diabetes. It has a traditional medicine effect. The plants of the genus Salachia include, in addition to Salachia reticulata, Salachia oblonga, Salacia chinensis, Salacia macrophylla, Salacia exculpta, and Salachia prinoides (Sa).
lacia prinoides), Salakia undurata (Salac)
ia undulata) are known.

[0003] In recent years, various studies on plants of the genus Salachia and extracts thereof have been reported. For example,
Aqueous extract from the roots and stems of Salachia reticulata inhibits blood glucose elevation during carbohydrate loading in rats and humans [Shimoda et al., Journal of the Japanese Society of Nutrition and Food Science, Vol. 51, No. 2,
79-287 (1998)] and prevention of hyperlipidemia [Shimoda et al., Journal of Japanese Society of Nutrition and Food Science, 53, 149-154 (2000)
Year)], safety [Shimoda et al., Food Hygiene Magazine, 40, 19
8-205 (1999)]) and blood glucose improving effect in patients with borderline diabetes [Kajimoto et al., Journal of Japan Society of Nutrition and Food Science, 53
Vol., Pp. 199-205 (2000)]. Furthermore, it has been found that water and methanol extracts from Salachia reticulata also have an effect of suppressing hepatocellular injury due to hepatotoxicants [Japanese Patent Application No. 2000-20993 filed on Jul. 6, 2000; and Shimoda et al. Authors, Abstracts of the 17th Annual Meeting of the Japanese Society of Pharmaceutical Sciences, 63 pages]. In addition, detailed studies have been made on pharmacologically active components related thereto, for example, regarding the blood sugar level-inhibiting effect, a novel thiosugar sulfonium sulfate inner salt structure as a component having high α-glucosidase inhibitory activity has been proposed. Salacinol [Yoshikawa M. et al., Tetrahedron Let
t., 38, 8367-8370 (1997)] and kotalanol (kot
alanol) [Yoshikawa M. et al., Chem. Pharm. Bull., 46
Vol., Pp. 1339-1340 (1998)] were isolated from Salachia reticulata, and their chemical structures have also been elucidated. These two components have also been isolated and identified from Salachia oblonga [Matsuda H. et al., Che.
m. Pharm. Bull., 47, 1725-1729 (1999)].

[0004] As the effectiveness of such plants of the genus Salachia and their extracts against lifestyle-related diseases such as diabetes, obesity and hyperlipidemia has become apparent, the demand for their use as, for example, health maintenance foods has also increased. Is growing. However, in practice, all the plants of the genus Salachia currently distributed in Japan are native to India, Sri Lanka, etc., and the quality control is not performed sufficiently during the growth process and import route. , Mold may be formed or deteriorated.
When such low-quality plants of the genus Salachia are used, desired medicinal effects may not be obtained, or extraction of necessary active ingredients may be difficult or impossible. for that reason,
Before use, it is essential to determine the quality of the plant of the genus Salachia.

As a criterion for judging the quality of a plant belonging to the genus Salachia, for example, the quantification of the aforementioned α-glucosidase-inhibiting active ingredients, salacinol and kotalanol, can be considered. However, according to the quantification of these components, only a trace amount is present in any plant of the genus Salachia,
In addition, it is difficult to detect and has low reproducibility due to low reactivity with the coloring reagent and no absorption in the wavelength region of ultraviolet and visible light; There are various problems in quality judgment, such as difficulty in derivatization for quantification in chromatography, high performance liquid chromatography, and the like.

[0006]

SUMMARY OF THE INVENTION Accordingly, an object of the present invention was to find a criterion for more easily distinguishing the quality of a plant of the genus Salachia and an extract therefrom. In addition, the present inventors have also found a criterion for confirming that a plant of the genus Salachia or an extract therefrom is formulated, even in a formulation or a food.

[0007]

Means for Solving the Problems In order to solve the above-mentioned problems, the present inventors have investigated the components in a plant of the genus Salachia and found that each component (for example, roots and stems) of the plant of the genus Sarachia Mangiferin, a xanthone glycoside known to contain:

Embedded image It is noted that the fact that this is contained in a certain level or more means that the plant of the genus Salachia and the extract therefrom are of good quality, that is, the aforementioned characteristic activities and actions (for example, carbohydrates) Blood glucose rise suppression during loading, etc.)
It has been found that this can be one of the indices for achieving the above, and the present invention has been completed.

Accordingly, the present invention is a method for judging the quality of a plant belonging to the genus Salachia and / or an extract therefrom using the presence of mangiferin as a quality judgment criterion. In the method of the present invention, mangiferin is
It is present in Salachia plants in an amount of at least 0.1% by weight of the total solids, or in extracts from Sarachia plants in an amount of at least 0.9% by weight of the total solids. Is a criterion for determining good quality.

[0009] The present invention also relates to a method for determining the presence of mangiferin in a preparation or food in which a plant belonging to the genus Euonymus and the extract thereof is formulated.
Methods for determining the quality of the formulation or food are also provided.

[0010]

DETAILED DESCRIPTION OF THE INVENTION Manxiferin, a xanthone glycoside, has the following structural formula:

Embedded image An example of isolation from the root bark of Salachia reticulata [Karunanayake EH et al., J. Ethnopharmacology, 1
3, 227-228 (1985)]. Its biological activity also has an antioxidant effect [Sanchez GM et al., Ph.
armacol. Res., 42, 565-573 (2000)], bone resorption inhibitory action [Li H. et al., Biol. Pharm. Bull., 21, 1322-13.
26 (1998)], immunomodulatory effects [Chattopadhyay U. et al., Ca
ncer Lett., 37, 293-299 (1987)], and hypoglycemic action [Ichiki H. et al., Biol. Pharm. Bull., 21, 13
89-1390 (1998)]. Mangiferin is a plant of the genus Salachia other than the aforementioned Salachia reticulata (eg, Salachia oblonga, Salachia reticulata).
It is known that it is also contained in various parts (for example, roots and trunks) of Kinensis, Salachia macrophylla, Salachia excluputa, Salachia purinoides, Salachia undurata and the like.

[0011] Mangiferin is contained in plants of the genus Salachia and extracts thereof, and has the above-mentioned characteristic activities and effects (for example, an effect of suppressing an increase in blood glucose level during carbohydrate load,
It is not a direct component capable of exerting a hyperlipidemia-preventing action, a blood sugar level-improving action in patients with borderline diabetes, etc.). However, in the present invention, under the condition that the plant of the genus Salachia or an extract therefrom can sufficiently exhibit the activity and action, the presence of mangiferin in a certain amount or more is always considered as a quality criterion. By doing so, it is possible to provide a method for easily determining the quality of the product with high reproducibility.

That is, the present invention is basically a method for judging quality based on a quality judgment criterion that the content of mangiferin is not less than a certain level. The content of mangiferin varies depending on whether the mother body to be determined is a plant of the genus Salachia (for example, roots and stems) or an extract therefrom. For example, the plant is a plant of the genus Salachia,
Specifically, when its roots, stems or leaves, mangiferin has at least 0.1 relative to total solids in the mother.
If it is contained in an amount of% by weight, the quality of the mother body is judged to be good. When the parent is an extract from a plant of the genus Salachia (for example, an aqueous extract or an alcoholic solvent extract), the total solid content in the extract is
If it is contained in an amount of at least 0.9% by weight, the quality of the base is judged to be good.

[0013] As a plant of the genus Salachia that can be used as a parent, if the plant contains a component capable of exhibiting a desired action or activity, the plant type or site (for example,
Leaf, bark, root bark, xylem, root xylem, etc.).

On the other hand, the extract from the plant of the genus Salachia as a parent may be water (particularly pure water) or an organic solvent as an extraction solvent. Examples of organic solvents include
Alcohols (eg, methanol, isopropanol, butanol, etc.); esters (eg, ethyl acetate, etc.); ketones (eg, acetone); ethers (eg, methyl ethyl ether, etc.) can be used. Two or more of these water or organic solvents may be used in combination, and a particularly preferred extraction solvent is water or ethanol. An example of the extraction method from the plant is as follows. After drying a desired part of the plant of the genus Salachia, a solvent is added, and the mixture is extracted while warming for 2 to 4 hours. The extract is filtered, and if necessary, the filtrate is heated to 40 ° C.
The desired extract can be obtained by removing the solvent under the following reduced pressure and concentrating or drying. As a mother, in the form of the filtrate after the above extraction, in the form of once concentrated or diluted, or in the form of its dry powder,
Alternatively, any form of a mixture thereof can be used.

In the present invention, the method and means for quantifying mangiferin in these plants and / or extracts thereof are not particularly limited, and high performance liquid chromatography (HPLC), gas chromatography (“GC”)
Although a method and a colorimetric method can be applied, quantification by the HPLC method is most preferable because it can be easily performed in a short time with good reproducibility.

As an example of the quantitative method by the HPLC method,
As a mobile phase, a mixed solution of methanol and an aqueous solution of acetic acid adjusted to pH 2 to 3 is used. For example, the volume mixing ratio of methanol and an aqueous solution of acetic acid is 15:85 v / v% to 65:35.
Gradient elution method changing to v / v% (gradient method)
And measured at a detection wavelength set to 360 nm. Thereby, for example, as shown in FIG. 1, in the fraction eluted with methanol: acetic acid aqueous solution = 50: 50 v / v%, mangiferin is detected at a peak retention time of about 12 minutes.

The determination method of the present invention can also be applied to a preparation or a food in which the above-mentioned plant of the genus Salachia and / or an extract thereof is prescribed. The method for quantifying the content of mangiferin in these preparations or foods may be performed in the same manner as described above.

The preparations to which the present invention can be applied are prepared by preparing the desired site of the plant of the genus Salachia and / or an extract therefrom alone or in combination of two or more. It may be something. The preparation may further contain other drugs (for example, crystalline cellulose, starch, erythritol, maltitol, lactose, talc, ethanol, purified water, etc.) as necessary, and may be a liquid, tablet, powder or capsule. It can be formed into a suitable form.

The concentration of the plant of the genus Salachia and / or the extract thereof contained in the preparation is not particularly limited as long as the desired action or activity can be exerted. If you use
It is preferably contained in an amount of at least 10% by weight based on the total weight of the preparation, and when an extract dry powder is used, the amount is preferably at least 1% by weight based on the total weight of the preparation.

In another embodiment of the present invention, the criterion for mangiferin content in the preparation may vary depending on whether a plant of the genus Salachia or an extract therefrom is prescribed in the preparation. For example, as a clue to the presence of a plant of the genus Salachia in a formulation, mangiferin may be at least 0.0% of the total formulation weight.
As an index, it is confirmed that the plant form of the genus Salachia is contained in the preparation at the above-mentioned preferable content. Alternatively, a clue to the presence of an extract from a plant of the genus Salachia in the formulation may be at least 0.000 of the total weight of the formulation.
The index indicates that mangiferin is contained in an amount of 1% by weight, which confirms that the extract from the plant of the genus Salachia is blended at the above-mentioned preferable content.

As a further aspect of the present invention, the present invention can be applied to foods containing plants of the genus Salachia and / or extracts thereof (eg, drinks, chewing gum, candy, jelly, etc.). The content of the plant of the genus Salachia and / or its extract in the food is not particularly limited as in the case of the above-mentioned preparations. For example, when a dry powder of a plant is used, 10% by weight of the total weight of the food is used. When the dry powder of the extract is used in the above amount or when the extract is used, it is preferably contained in an amount of 1% by weight or more of the total weight of the food.

[0022] In foods, depending on whether the ingredient is a plant of the genus Salachia or an extract therefrom,
The content of mangiferin to be quantified can vary, but if the form of the plant of the genus Salachia is incorporated in the preferred content described above, it will be present in an amount of at least 0.001% by weight of the total weight of the food, or the plant of the genus Salachia In the case where the extract is mixed at the above-mentioned preferable content, it is determined that mangiferin is contained in an amount of at least 0.001% by weight of the total weight of the food.

[0023]

EXAMPLES The present invention will be further described below with reference to examples, but the present invention is not limited to these examples. Example 1 (Mangif from Salakia Reticulata
Preparation of Erin ) Salachia reticulata (Place of origin: India;
1.0 kg of dried roots (hereinafter abbreviated as SR) was crushed, extracted with 10 L of water and refluxed at 100 ° C. for 3 hours. After the obtained extract was separated by filtration, the residue was washed with 1 L of water and combined with the extract. The extract was concentrated and spray-dried under reduced pressure (60 ° C., 750 mmHg) to obtain an aqueous extract (120.7
g, yield: 12.7%). The water extract (120 g) was dissolved in water (1.2 L) by heating at 60 ° C. for 30 minutes and centrifuged (3000 g).
(Revolution / min, 20 minutes), and the solvent was distilled off from the obtained supernatant under reduced pressure to obtain a water-soluble portion (84.8 g, 9.0%). This was used for Sephadex LH-20 column chromatography.
The mobile phase (water
Elution was performed using 100% → water: methanol = 50:50 → methanol 100%), and the water eluted portion (59.1 g,
6.6%), 50% methanol elution (8.3 g, 0.88%) and methanol elution (7.8 g, 0.89%). An operation consisting of normal-phase silica gel column chromatography, preparative high-performance liquid chromatography, and recrystallization was repeated on the 50% methanol eluted portion to obtain mangiferin (624 mg, 0.066%).

The obtained mangiferin contains ¹ H- and
^The product was identified to have the following structural formula by ¹³ C-nuclear magnetic resonance and mass spectrometry. This was used as a criterion in the following examples.

Embedded image

Example 2 (Extraction in SR dried root and its water)
Determination of mangiferin in foods) Preparation of water extract sample: 6 samples of dried root of SR (origin: India) (lot numbers: 981201, 9906A06, 9910A10,
9911B11, 9912B12, 0001A01) with hot water (100
C.) for 3 hours, filtered and concentrated to dryness to prepare an aqueous extract. After accurately weighing 0.1 g of this water extract and dissolving with distilled water to a volume of 100 mL,
The solution filtered through a membrane filter (pore size 0.45 μm) was subjected to the following quantitative analysis method.

2. Preparation of SR dried root sample:
The following quantitative analysis was performed on the solution obtained by extracting 1 g of each of 6 dried root samples of R with hot water (100 ° C.) for 3 hours, filtering the solution, making the volume 100 mL, and filtering the solution with a membrane filter (pore diameter 0.45 μm). Was performed.

3. Quantitative analysis method:-Quantitative analyzer (HPLC): Waters 2690 high-performance liquid chromatography (Waters Corporation)-UV-visible spectrophotometer detector: Waters 2487 (Waters Corporation)-Column: XTerra MS C18 (Waters Corporation) Corporation, filler: octadecylsilylated silica gel,
Column size: length 150 mm x inner diameter 3.9 mm, particle size of filler 5 μm) A mixed solution of methanol as a mobile phase and a 1% acetic acid aqueous solution was subjected to a linear gradient method (0 min, methanol: 1%
Acetic acid aqueous solution = 15:85 → 20 minutes, = 25:75 → 60 minutes, =
65:35) and flow at a flow rate of 1.0 mL / min.
The measurement was performed at 5 ° C. and a measurement wavelength of 360 nm (the injection volume of each water extract sample was 20 μL).

The mangiferin content in the above six specimens (ie, SR dried roots) and the water extract therefrom was as follows:
Each of the obtained chromatographs was determined by an absolute calibration curve method. Table 1 shows the results. Also Lot # 99
Figure 1 shows the chromatogram of the water extract from 10A10.
Shown in According to FIG. 1, the peak of mangiferin was clearly observed at a retention time of about 12 minutes. Similar peaks were observed for other lots at a retention time of about 12 minutes.

[0029]

[Table 1] ^* : Expressed as dry weight (%) based on the weight of extract or plant used.

According to the present invention, a simple quantitative determination by the HPLC method is carried out, and the specific value of mangiferin as a criterion is at least 0.9% by weight of the total solid in the case of an aqueous extract. Alternatively, when used as a plant, it was determined to be good if it was contained in an amount of at least 0.1% by weight of the total solids.

Example 3 (α-glucosidase inhibition test) Six dried root samples of SR used in Example 2 (# 981201, 9906)
A06, 9910A10, 9911B11, 9912B12, 0001A01) and,
Each aqueous solvent extract prepared therefrom in Example 2 was subjected to an α-glucosidase inhibition test using a rat jejunal brush border membrane crude enzyme according to the following procedure. After mixing each sample (50 μL) diluted with 0.1 M maleate buffer (pH 6.0) and 74 mM sucrose (100 μL), 37
Preincubation was performed at 3 ° C. for 3 minutes. To this, 50 μL of a crude enzyme diluted with a buffer solution (prepared so that the amount of glucose produced in the control group was 5 mg / dL) was added, and reacted for 30 minutes. After 30 minutes, purified water (800 μL) was added, and the reaction was stopped by immersing the test tube in a boiling water bath for 2 minutes. After cooling, the amount of produced glucose was measured using the mutarotase / glucose oxidase method (glucose CII Test Wako, Wako Pure Chemical Industries). The activity inhibition rate of sucrase, which is a sucrose hydrolase, was calculated by comparison with a control group. The obtained results are shown in Table 1 above as “50% α-glucosidase inhibitory concentration (IC ₅₀ )”.

Further, the content of mangiferin in the extract of each lot and the 50% α-glucosidase inhibitory activity (IC ₅₀ )
2 is shown in FIG. A high correlation was observed between the mangiferin content and the α-glucosidase inhibitory activity, indicating that the present quantification method can be a criterion for the quality of Salachia reticulata.

Also in this example, mangiferin as a criterion is, as a specific numerical value, not less than 0.9% by weight of the total solids in the case of an aqueous extract, or in the case of being used as a plant. A good product can be determined if it is contained in an amount of 0.1% by weight or more of the solid content.

Example 4 (methods from various plants of the genus Salachia)
Determination of Mangiferin in Tanol Extract) Preparation of methanol extract sample: Plant 4 of the genus Salachia
Methanol extracts were prepared by extracting the roots of seeds (SR, Salachia oblonga, Salachia purinoides and Salachia quinensis) with methanol (80 ° C, 3 hours), filtering and concentrating to dryness, respectively. The yield is shown in Table 3 in terms of the solid content based on the plant weight. Each 0.1 g of these methanol extracts was accurately weighed and dissolved in a mixed solution of methanol and water (volume mixing ratio = 1: 1 v / v) to make 100 mL. This was filtered through a membrane filter (pore size: 0.45 μm), and the solution was used as an analysis sample. The solution was subjected to the same quantitative analysis method as in Example 2 to quantify the mangiferin content. Table 2 shows the obtained results.

2. Preparation of each dried root sample: Further, 1 g of each dried root of the plant was dissolved in methanol, filtered, and filtered.
After adjusting the volume to 0 mL, the membrane filter (pore size 0.45
(μm) was subjected to the same quantitative analysis as in Example 2. Table 2 shows the results.

[0036]

[Table 2] ^* : Expressed as dry weight (%) based on the weight of extract or plant used.

All the chromatograms of the methanol extract from each plant of the genus Salachia are shown in FIG.
It resembled the pattern of the water extract from R (lot # 9910A10). From the results in Table 3 above, the amounts described in Example 2 (at least 0.9% by weight of the total solids in the case of the methanol extract, or the plant extract) were also determined for each Salachia plant and each methanol extract therefrom. At least 0.1% by weight of total solids when used as is)
Showed that mangiferin was present, and that the criteria of the present invention were applicable to other plants of the genus Salachia.

Example 5 (Matrix in tablet formulation containing SR )
Quantification of ngiferin) Commercial SR-containing tablet formulation [Morishita Jintan Co., Ltd., trade name “Salacia (registered trademark) diet granules”; composition = S
Water extract dry powder from R (1 to total solids in the formulation)
3.3% by weight), erythritol, maltitol, sucrose ester, and thrombus extract] were collected, pulverized, and classified by a No. 50 sieve (pore size: 300 μm) to obtain a sample. After accurately weighing 0.1 g of the sample, 10 mL of distilled water was added and stirred under ultrasonic irradiation to prepare a uniform suspension.
This is centrifuged (3000 rpm or more, 10 minutes, room temperature), and the resulting supernatant is passed through a membrane filter (0.45 μm pore size).
The product filtered in m) was used for analysis. Quantification by high performance liquid chromatography was performed under the same conditions as in Example 2. FIG. 3 shows the obtained chromatogram. In FIG. 3, peak 1 indicates mangiferin (retention time 12 minutes).

According to the present invention, mangiferin derived from SR in the preparation can be separated and quantified well, and as a result, it has been clarified that 0.50 mg of mangiferin is contained per 1 tablet (300 mg) of the preparation. Was. From these results, it can be determined that this preparation can incorporate an extract from a plant of the genus Salachia in a sufficient amount.

Example 6 (Mangiferi in food containing SR)
Quantitative determination) Commercially available drink containing salachia reticulata [trade name “Salacia (registered trademark) diet drink; manufactured by Morishita Jintan Co., Ltd .; composition = water extract dry powder from SR: 0.16 weight based on the solid content of all foods” %), Tea extract powder, erythritol, citric acid and purified water] 2mL
Was directly filtered using a membrane filter (pore size: 0.45 μm) to obtain a sample. Quantification by high performance liquid chromatography was performed under the same conditions as in Example 2. as a result,
The chromatogram shown in FIG. 4 was obtained. In FIG. 4, peak 1 shows mangiferin (retention time 12 minutes).

Compared with the result of Example 5 (FIG. 3), the chromatogram shown in FIG. 4 has a plurality of peaks derived from tea extract powder, but in the present invention, mangiferin observed at a retention time of about 12 minutes was used. Focusing on the peak, the content of mangiferin can be quantified therefrom, and the amount can be used to confirm the presence of a plant of the genus Salachia and / or an extract thereof. In the case of this drink, 1 bottle (50 mL)
Since it was found that 1.30 mg of mangiferin was contained per food, it can be determined that this food can be mixed with a sufficient amount of extract from a plant of the genus Salachia.

[0042]

According to the present invention, the quality of a plant belonging to the genus Salicaceae and / or an extract therefrom is determined by applying the content of mangiferin as a criterion for quality determination. it can. Also,
This method is sufficiently applicable to the determination of the quality of a preparation or food that may contain a plant of the genus Salixia and / or an extract therefrom. Furthermore, the measurement results of the mangiferin content in the extract from the plant of the genus Salachia and α-
It was also found that the glucosidase inhibitory activity (IC ₅₀ ) showed a very high correlation. That is, it was also confirmed that the measurement of the content of mangiferin, which is the quality criterion of the present invention, is an effective alternative to the conventional bioassay that requires time and labor.

[Brief description of the drawings]

FIG. 1 is an HPLC chromatograph of an aqueous extract from dried roots of Salachia reticulata (lot # 9910A10) in Example 2 of the present invention.

FIG. 2 is a graph showing the correlation between mangiferin content and α-glucosidase inhibitory activity (IC ₅₀ ) for the water extract of Salachia reticulata tested in Example 3.

FIG. 3 is a HPLC chromatograph of a commercially available tablet food containing Salachia reticulata in Example 5.

FIG. 4 is a HPLC chromatograph of a commercially available drink containing Salachia reticulata in Example 6.

[Explanation of symbols]

 1: Mangiferin peak

──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) // A23L 1/30 A23L 1/30 B A61K 35/78 A61K 35/78 C X (72) Inventor Nishida Norinaga 1-30, Tamatsukuri, Chuo-ku, Osaka-shi, Osaka Inside Morishita Nittan Co., Ltd. (72) Inventor Miki Takada, 1-1-1 Tamazo, Chuo-ku, Osaka-shi, Osaka F-term in Morishita Nittan Co., Ltd. (reference) 4B018 MD61 ME01 ME03 ME04 MF14 4C088 AB12 AC05 AC11 BA08 BA13 CA03 MA52 NA14 NA20 ZA70 ZC33 ZC35

Claims (5)

[Claims] 1. A method for judging the quality of a plant belonging to the genus Salicaceae and / or an extract therefrom, wherein the quality is based on the inclusion of mangiferin having the following structural formula. Embedded image 2. The plant of the genus Salicaceae according to claim 1, wherein the quality criterion is that mangiferin is contained in the plant of the genus Salachia in an amount of at least 0.1% by weight of the total solids. Quality judgment method. 3. The quality criterion is that mangiferin is contained in an extract from a plant of the genus Salachia in an amount of at least 0.9% by weight of the total solids.
A method for judging the quality of an extract from a plant of the genus Salixia belonging to the family Euonymus. 4. A plant of the genus Salachia of the family Euonymus and an extract therefrom, wherein the quality criterion is to confirm the presence of mangiferin in a preparation in which the plant of the genus Salicaceae and an extract thereof are formulated. Quality evaluation method of the drug product. 5. A plant belonging to the genus Salachia of the family Euonymus and an extract thereof, the quality of which is determined by confirming the presence of mangiferin in the food containing the plant of the genus Salachia and the extract thereof. Food quality judgment method.