JP2002212084A - Antioxidative substance - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an antioxidative substance which contains a farm product such as a vegetable as a raw material and has excellent medicinal efficacies such as an excellent carcinostatic efficacy. SOLUTION: This antioxidative substance comprises vitamins and a farm product cultivated under the application of a chelate compound-containing fertilizer. When the antioxidative substance is taken in a body, enzymes comprising glutathione S-transferase molecule species (GST) and glutathione peroxidase (GPx) are induced.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to an antioxidant, particularly an antioxidant derived from a crop grown by applying a fertilizer containing a chelate compound.

[0002]

2. Description of the Related Art In recent years, with the health consciousness of consumers, safe and high value-added foods have been demanded, and many natural foods, health foods, nutrients and the like have been developed. JP-A-8-4092
No. 3 publication contains a mineral mixture having a mineral composition ratio similar to that of the primitive sea, an amino acid, a vitamin, a substance having a scavenging action and / or an antioxidant ability, a phenol compound, and a sugar compound such as a flavonoid and a sugar flavonoid. Shows nutritional supplements that prevent adult diseases. Agricultural crops such as vegetables and fruits are also affected by the recent natural food boom, and cultivation techniques such as organic farming and breeding have been improved, and components of fertilizers have been improved.

[0003]

The fertilizer does not contain harmful substances and contains a large amount of fertilizing components, enables mass production of agricultural crops, and has low hygroscopicity or caking, and is easy and inexpensive to handle. It is desired. In recent years, agricultural products with high commercial value that meet the needs of consumers have been demanded.
Cultivation of safe and medicinal crops is required in line with health consciousness. Therefore, the present invention uses agricultural crops as raw materials,
An object is to provide a safe antioxidant without side effects. It is another object of the present invention to provide an antioxidant that enhances antioxidant action and detoxifies a causative substance such as cancer in the body.

[0004]

An antioxidant according to the present invention comprises a crop grown by applying a fertilizer containing a chelate compound, vitamin A, vitamin B ₁ , vitamin B ₂ , vitamin B ₃ , pantothenic acid, vitamin B _6, vitamin B _12, folic acid, inositol choline, biotin, vitamin C, vitamin D, and a one or more vitamins of the vitamin E and vitamin K, when ingested glutathione S
-Induces enzymes of transferase species (GST) and glutathione peroxidase (GPx).

[0005] Peroxides such as hydrogen peroxide and organic peroxides covalently bind to nucleophilic parts of DNA and proteins to cause mutation, carcinogenesis, and cytotoxicity. And the generated peroxide must be decomposed. When a crop grown by applying a fertilizer containing a chelate compound is ingested into the body, enzymes of glutathione S-transferase molecular species (GST) and glutathione peroxidase (GPx) are produced in the body.
These enzymes act on peroxy radicals generated during oxidation to stop the peroxide-forming oxidation reaction and decompose peroxides in the body. In particular, in the present invention containing a vitamin, the vitamin acts as a synergist for GST and GPx enzymes having antioxidant activity. Synergists are substances that have no direct antioxidant action, but enhance their resistance when co-existing with antioxidants. For this reason, when an antioxidant composed of vitamins and vitamins to which a fertilizer containing a chelate compound is applied is ingested into the body, the production of peroxide can be completely suppressed by the synergistic effect of vitamins. In addition, hydrogen peroxide and organic peroxides in the body can be inactivated by breaking the bond between oxygen and reduced to compounds such as water and small molecular weight alcohols, so peroxide is generated in the body. Mutations, carcinogenesis,
Cell toxicity and the like can be prevented. Furthermore, in the present invention, since a crop cultivated by fertilizing a harmless chelate compound is used as a raw material, it can be safely taken into the body without side effects caused by administration of a synthetic drug.

[0006]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, embodiments of the antioxidant according to the present invention will be described. The antioxidant according to the present invention includes a crop and a vitamin cultivated by applying a fertilizer containing a chelate compound. Vitamins are different from carbohydrates, lipids and proteins that are the components of the body and energy sources.
Although a trace amount has the effect of smoothly performing metabolism and physiological phenomena in a living body, in the present invention, vitamins also act as synergists to enhance the antioxidant effect of antioxidants. When the antioxidant of the present invention is taken into the body, glutathione S-
Induces transferase species (GST) and glutathione peroxidase (GPx) enzymes. These enzymes have an antioxidant effect, and when vitamins coexist with these enzymes, they reliably decompose highly reactive electrophilic compounds such as peroxides and active oxygen in the body. Vitamins are vitamin A, vitamin B _1, vitamin B _2, vitamin B _3,
One or more of pantothenic acid, vitamin B ₆ , vitamin B ₁₂ , folic acid, inositol choline, biotin, vitamin C, vitamin D, vitamin E and vitamin K can be used.

[0007] The fertilizer containing a chelate compound is, on a weight basis,
Metal compound 0.0 forming the central metal of the chelate compound
1-4 parts, 1-5 parts of the amino acid mixture, and nitrogen fertilizer 0.4.
1 to 1 part, and 0.01 to 2 parts of a phosphorus fertilizer, the pH becomes 6.0 to 7.5 when mixed with water, and the amino acid mixture is a mixture of an amino acid and a sugar acid, and a mixture of an amino acid and a fatty acid. Or a mixture of amino acids, sugar acids and fatty acids.
In amino acids, the nitrogen atom of the amino group has a strong electron-donating property and promotes coordination bond with a metal which is an electron acceptor. Therefore, when an amino acid is contained, a chelate compound with a metal is easily formed. In addition, when an amino acid forms a chelate compound with a metal, the carboxyl group and the amino group contained in one molecule of the amino acid generally form a bidentate complex to form a 5-membered chelate ring. Complexes containing a chelate ring have higher stability than the corresponding simple complex, and the five-membered ring is the most stable (chelate effect). Therefore, a chelate compound formed by mixing an amino acid and a metal is left for a long time. However, no precipitation of the metal compound occurs and no denaturation occurs. In addition, when sprayed as fertilizer in the soil, the chelate compound is surely absorbed by the crop without being substantially decomposed by microorganisms, etc., and when the crop is taken into a living body, a catalyst that reduces active oxygen and the like. Can be produced in high yield. As a result, crops having good color and fragrance and having an antiseptic and antifungal action are cultivated. As the amino acid, one or more of glycine, alanine, phenylalanine, valine, arginine, lysine, aspartic acid, glutamic acid, serine, threonine, tyrosine, methionine, cysteine, isoleucine, leucine, tryptophan and valine are used.

If the amount of the metal compound is less than 0.01 part, no chelate compound is formed, and if the amount of the metal compound exceeds 4 parts, the effect as a fertilizer containing the chelate compound is not changed, but an excess amount of the metal compound is not contained in the soil. Must be prevented from accumulating. If the total amount of one or both of the amino acid and the sugar acid and the fatty acid is less than 1 part, no chelate compound is formed, and the effect does not change even if it exceeds 5 parts. When the content of nitrogen fertilizer is 0.1 to 1 part, it promotes plant protein synthesis, cell division and proliferation, root development, leaf stem prosperity, and promotes nutrient absorption and assimilation. . 0.1
If the amount is less than 10 parts, the protein cannot be sufficiently synthesized. When the content of the phosphorus fertilizer is 0.01 to 2 parts, the root growth is promoted and germination is activated. If the amount of the phosphorus fertilizer is less than 0.01 part, the root growth of the crop is not sufficiently promoted. When the nitrogen fertilizer and the phosphorus fertilizer exceed 1 part and 2 parts, respectively, the physiological action of each fertilizer does not change, but an excessive amount of the fertilizer remains in the soil and adversely affects the soil. If the pH of the chelate compound-containing fertilizer is less than 6.0, soil contamination may occur due to excessive acidity, and if it exceeds 7.5, the stability of the complex is affected.

The metal compound is a metal salt, metal oxide or metal hydroxide of sulfate, chloride, nitrate, phosphate, acetate, carbonate, silicate or borate. The metal of the metal compound is phosphorus (P), manganese (Mn), zinc (Zn), copper (Cu), molybdenum (Mo), iron (Fe), selenium (Se), chromium (Cr), cobalt (Co) , Vanadium (V), lithium (Li), nickel (Ni), titanium (T
i), gallium (Ga), silver (Ag), strontium (S
r), indium (In), tin (Sn), gold (Au), zirconium (Zr), aluminum (Al) and germanium (Ge). A chelate compound-containing fertilizer containing a metal necessary for growing the crop or a metal necessary for the body when the crop is ingested can be produced, so that the metal content can be artificially controlled as necessary.

[0010] The fertilizer containing a chelate compound is produced by mixing each substance at the following ratio. For example, on a weight basis, 0.03 to 2 parts of selenium trioxide, 0.5 to 1.5 parts of amino acid, 0.5 to 1.5 parts of pentanoic acid, and 0.5 to 1.5 parts of acetic acid. And 0.3 to 0.8 parts of nitrogen fertilizer and 0.5 to 1 part of phosphorus fertilizer, and water is mixed to adjust the pH to 6.3 to 7.0. Also, on a weight basis, 0.05 to 2 parts of germanium acetate,
1.5 to 2.5 parts of glyceric acid and 0.3 to 0.8 of nitrogen fertilizer
Parts and 0.5 to 1 part of phosphorus fertilizer,
Is set to 6.3 to 7.0.

As the sugar acid, pentalic acid, hexalic acid (including gluconic acid) and the like can be used. Fatty acids are formic, acetic, propionic, lauric, myristic, palmitic,
Saturated fatty acids such as stearic acid, and unsaturated fatty acids such as acrylic acid, crotonic acid, oleic acid, sorbic acid, linoleic acid, and linolenic acid can be used.

Nitrogen fertilizers include ammonium sulfate ((NH ₄ ) ₂ SO ₄ ) and salt ammonium (N
H ₄ Cl), nitrate (NH ₄ NO ₃ ), phosphorus ((NH ₄ ) ₂ HPO ₄ , NH ₄ H ₂ P
O ₄ ), ammonia water fertilizer (NH ₄ OH), ammoniated day coal (ammonium salt of organic acid), ammoniated superphosphate (NH ₄ H ₂ PO ₄ , (NH ₄ ) ₂ SO ₄ ), ammonium nitrate ((NH ₄ ) ₂ SO ₄ + NH ₄
NO ₃ ), ammophos ((NH ₄ ) ₂ SO ₄ + (NH ₄ ) ₂ HPO ₄ ), ammonium phosphate ((NH ₄ ) ₂ SO ₄ + (NH ₄ ) ₂ HPO ₄ ), fertilizer (ammonium salt) And ammonia nitrogen such as fertilizer (ammonium salt). Ammonium nitrate (NH ₄ NO ₃ ), ammonium nitrate ((NH ₄ ) ₂ SO ₄ + NH ₄ NO ₃ ), nitrogen nitrate. Urea (CO (NH ₂ ) ₂ ), urea resinized fertilizer (uraform) (CO (NH ₂ ) ₂ -HCHO condensate), oxamide (NH ₂ · CO
・ CO ・ NH ₂ ), guanylurea salt ([NH ₂・ C (NH) ・ NH ・ CO ・
NH ₂ ] .X), guanidine salt ([NH ₂ .C (NH) .NH ₂ ] .X),
Amino nitrogen such as ammoniated day coal (containing CO (NH ₂ ) ₂ ). Protein nitrogen such as fish manure (containing NH ₂ and NH) and soybean scum (containing NH ₂ and NH) can be used. (X: HCl, H ₂ SO ₄ , H
NO ₃ , H ₃ PO ₄ etc.) Phosphorus fertilizer is ammonium sulfate ((NH ₄ ) ₂ SO ₄ + NH ₄ H
₂ PO ₄ ) etc. can be used.

[0013]

EXAMPLES Hereinafter, the present invention will be described specifically with reference to examples, but the examples do not limit the present invention in any way. All parts are by weight. Example 1 After dissolving 0.07 parts of selenium trioxide (SeO ₃ ) in ₃ parts of water, 1 part of amino acid, 1 part of pentalic acid and 1 part of acetic acid
Was added to a mixed solution of 3 parts of water and stirred for 60 minutes to completely dissolve. Ammonium sulfate ((N
0.5 parts of H ₄ ) ₂ SO ₄ ) and ammonium phosphate ((NH ₄ ) ₂
SO ₄ + NH ₄ H ₂ PO ₄ ) was added to obtain a chelate compound-containing fertilizer whose pH was adjusted to 6.6. The obtained chelate compound-containing fertilizer was diluted 10-fold with water, and sprayed at the base of broccoli strains 100 cc once a week for 2 months. The broccoli cultivated with the fertilizer containing the chelate compound was freeze-dried, and 100 parts of powdered broccoli crushed and classified were mixed with 5 parts of vitamin A to obtain an antioxidant.

Example 2 4 parts of iron acetate was dissolved in 3 parts of water, and 2 parts of glyceric acid was dissolved in 3 parts of water and mixed.
Stir for 0 min to completely dissolve. To this mixture, 0.3 part of an ammonia water fertilizer as a nitrogen fertilizer and 0.6 part of phosphorus ammonium sulphate ((NH ₄ ) ₂ SO ₄ + NH ₄ H ₂ PO ₄ ) as a phosphorus fertilizer were added and stirred to adjust the pH. A fertilizer containing a chelate compound adjusted to 6.5 was obtained. The resulting chelate compound-containing fertilizer was diluted 10-fold with water, and sprayed at the base of brussels sprouts strain once a week at 100 cc for 2 months. The antibacterial substance was obtained by mixing 100 parts of powdery brussels sprouts obtained by freeze-drying and pulverizing and classifying the brussels sprouts cultivated with the fertilizer containing a chelate compound and 7 parts of vitamin E.

[Test Method] The antioxidant obtained in Example 1 and the antioxidant obtained in Example 2 were used as Comparative Examples 1 and 2, and broccoli cultivated without a fertilizer containing a chelate compound. And Brussels sprouts (without mixing vitamins)
And the color, scent,
The amounts of generated enzymes having taste, antiseptic and antifungal effects, detoxification of cancer and the like, and antioxidant effects were compared. (1) The color and fragrance were observed under normal conditions. (2) Placed in a container filled with water and allowed to stand at room temperature, and tested for antiseptic and antifungal action. (3) After feeding to F344 female rats (7 weeks old) for 3 weeks,
The liver and kidney were excised, and the amounts of enzymes contained in these organs, which detoxify cancer and the like and have antioxidant effects, in particular, glutathione S-transferase (GST) molecular species and glutathione peroxidase (GPx) were measured.

Glutathione S-transferase (G
ST) The method for measuring the molecular species is as follows. The extracted organ was homogenized by adding three times the weight of 0.25 M sucrose-10 mM phosphate buffer (pH 7.4). 50 μl of 20% SDS and PB in 20 μl of the homogenized sample
Add 275 μl of S, dilute 10 times with water,
Total protein was measured by the method y). The mixture was adjusted to a protein concentration of 1 mg / ml with a sample diluent, boiled in boiling water for 2 minutes, and 50 μl of 0.1% BPB was added to prepare an electrophoresis sample. 12.5% SDS-polyacrylamide gel (electrophoretic SDS-PAGE) was used and the number of stacking gels was 2
The separating gel ran at 36 mA for 1 hour until it passed through the stacking gel at 8 mA. BIO RAD Trans Blot Cell (TRANS-BLOT CE)
LL) at 80 V for 2 hours and transfer the proteins separated by electrophoresis onto a nitrocellulose membrane (Western Bl
ot).

[0017] Nitrocellulose membrane is 3% skim milk TBS
Blocking treatment was performed for 1 hour with a buffer solution (pH 7.4). rG
STA1 (Ya), rGSTA3 (Yc), rGSTA4 (Y
k), rGSTM1 (Yb1), rGSTM2 (Yb2), rGS
Biotrin International Limited for each of TP1 (Yp)
d) Rabbit polyclonal antibody (manufactured by the company) was washed twice with TBS buffer containing 3% bovine albumin (BSA) for 5 minutes, with TBS buffer containing 0.05% Tween 20 twice for 5 minutes, and then with TBS.
The primary antibody was washed with buffer for 5 minutes.

[0018] Jackson Immuno Research Laboratories, Inc.
ch. Laboratories, Inc.) with an alkaline phosphatase-labeled anti-rabbit IgG goat antibody in a TBS buffer (pH 7.4).
After diluting 000-fold and reacting at room temperature for 1 hour, the secondary antibody was washed in the same manner as the primary antibody. A color reaction was performed using Nitro Blue Tetra (Pierce) as a color reagent. Import the color band of each sample into Adpi Photoshop of photo retouching software, NI
The intensity of the coloring band was measured with an H image analyzer. The obtained results were compared, and the state of induction of GST molecular species by a significant difference test (t-test) was examined. The measured value of the GST molecular species was determined by F
The intensity ratio (without unit) was calculated assuming that the intensity of the coloring band for the liver and kidney of a 344 female rat (7 weeks old) was 1.

[Test Results] As shown in Table 1, the test results are as follows:
With respect to the color, aroma, taste, antiseptic and antifungal effects, Examples 1 and 2 showed remarkable effects in comparison with Comparative Examples 1 and 2, which were broccoli and brussels sprouts cultivated without applying a fertilizer containing a chelate compound. In addition, in Examples 1 and 2, the glutathione S-transferase (GST) molecular species showed significant differences in kidney and liver in Examples 1 and 2 with respect to the amounts of generated enzymes having detoxification and antioxidant effects on cancer and the like. Appeared. Glutathione peroxidase (GPx) showed a significant difference in the kidney. * Indicates that there is a significant difference compared to the comparative example. As a result, the antioxidant obtained by mixing the fertilizer containing the chelate compound of this example with the fertilizer, freeze-drying and pulverized and classifying broccoli and brussels sprouts with vitamin A and vitamin E is capable of detoxifying and antioxidizing cancer and the like in the body. Was found to generate an enzyme with

[0020]

[Table 1]

[0021]

According to the present invention, when a body cultivated by applying a fertilizer containing a chelate compound and an antioxidant according to the present invention containing a vitamin are taken into the body, harmful substances such as peroxides are surely decomposed and their production is reduced. Can be stopped,
It can detoxify cancer and the like and promote health.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI theme coat ゛ (Reference) A61K 31/122 A61K 31/122 4H061 31/14 31/14 31/197 31/197 31/355 31/355 31/375 31/375 31/4188 31/4188 31/4415 31/4415 31/455 31/455 31/51 31/51 31/519 31/519 31/525 31/525 31/59 31/59 31 / 592 31/592 31/724 31/724 A61P 39/06 A61P 39/06 43/00 111 43/00 111 C05G 1/00 C05G 1/00 H 3/00 3/00 Z // A23L 1/30 A23L 1 / 30 BF term (reference) 2B150 DA43 DA71 DA72 DA76 DD44 DD56 4B018 MD01 MD05 MD06 MD23 MD48 ME06 4C086 AA01 AA02 BA09 BA18 BC18 BC83 CB09 CB28 DA15 DA39 MA02 MA04 NA14 ZC02 ZC19 4C088 AC01 BA37 MA03 NA14 A20A02A19 FA42 GA05 GA36 MA02 MA04 NA14 ZC02 ZC19 4H061 AA01 BB01 BB10 BB21 BB32 DD20 EE11 EE27 EE41 EE70 FF24 HH 07 LL25 LL26

Claims (7)

[Claims] 1. A crop grown by applying a fertilizer containing a chelate compound, vitamin A, vitamin B ₁ , vitamin B ₂ , vitamin B ₃ , pantothenic acid, vitamin B ₆ , vitamin B ₁₂ , folic acid, inositol choline, Contains one or more vitamins of biotin, vitamin C, vitamin D, vitamin E and vitamin K, and induces glutathione S-transferase molecular species (GST) and glutathione peroxidase (GPx) when taken into the body Antioxidants. 2. The fertilizer containing a chelate compound comprises, on a weight basis, 0.01 to 4 parts of a metal compound forming a central metal of the chelate compound, 1 to 5 parts of an amino acid mixture, and 0.1 to 1 part of a nitrogen fertilizer. And 0.01 to 2 parts of a phosphorus fertilizer, and when mixed with water, the pH becomes 6.0 to 7.5. The amino acid mixture is a mixture of an amino acid and a sugar acid, a mixture of an amino acid and a fatty acid or an amino acid, a sugar acid. The antioxidant according to claim 1, wherein the antioxidant is a mixture of carboxylic acids and fatty acids. 3. The metal compound is a metal salt, metal oxide or metal hydroxide of sulfate, chloride, nitrate, phosphate, acetate, carbonate, silicate or borate. Item 3. An antioxidant according to Item 2. 4. The metal of the metal compound is phosphorus (P), manganese (Mn), zinc (Zn), copper (Cu), molybdenum (M
o), iron (Fe), selenium (Se), chromium (Cr), cobalt (Co), vanadium (V), lithium (Li), nickel (Ni), titanium (Ti), gallium (Ga), silver ( Ag),
Strontium (Sr), Indium (In), Tin (S
n), gold (Au), zirconium (Zr), aluminum (A
The antioxidant according to claim 2, which is one or more of l) and germanium (Ge). 5. The amino acid is one or more of glycine, alanine, phenylalanine, valine, arginine, lysine, aspartic acid, glutamic acid, serine, threonine, tyrosine, methionine, cysteine, isoleucine, leucine, tryptophan and valine. The antioxidant according to claim 2, comprising: 6. Selenium trioxide 0.03 to 2, based on weight.
Parts, 0.5-1.5 parts of amino acids, 0.5-
1.5 parts, acetic acid 0.5-1.5 parts, nitrogen fertilizer 0.3-
The antioxidant according to claim 2, comprising 0.8 part and 0.5 to 1 part of a phosphorus fertilizer, and having a pH of 6.3 to 7.0 when mixed with water. 7. Germanium acetate 0.05 on a weight basis.
２2 parts, 1.5-2.5 parts of glyceric acid, 0.3-0.8 parts of nitrogen fertilizer, and 0.5-1 part of phosphorus fertilizer, and when mixed with water, the pH is 6.5. 3. The antioxidant according to claim 2, which has a value of 3 to 7.0.