KR19990084146A - Fertilizer containing chelate compounds - Google Patents

Abstract

(1) The technical field to which the invention belongs

The present invention relates to the technical field belonging to fertilizers, especially fertilizers containing amino acids, sugar acids and chelate compounds formed by fatty acids and metals.

(2) Purpose of invention

The present invention is to provide a fertilizer capable of growing crops, such as vegetables, such as good color, aroma, taste, preservative, fungal action, and particularly rich in anticancer effects.

(3) Composition of invention

These fertilizers include 0.01 to 4 parts of metal compounds, 1 to 5 parts of amino acid mixtures, 2 to 8 parts of fertilizers, 0.1 to 1 parts of nitrogen fertilizers, and 0.01 to 2 parts of phosphate fertilizers. The pH is 6.0 to 7.5 and the amino acid mixture is composed of sugar acid, fatty acid or sugar acid, and a mixture of fatty acid and amino acid.

(4) effects of the invention

Crops grown by fertilizing the fertilizer containing a chilate compound according to the present invention have good color, aroma, and taste, and have antiseptic and mildew effects, so that quality is not degraded even when transported to a remote site for a long time. In addition, when ingested in vivo, it causes enzymes having detoxification and antioxidant effects such as cancer, thereby improving health.

Description

Fertilizer containing chelate compounds

The present invention is to provide a fertilizer capable of growing crops, such as vegetables, such as good color, aroma, taste, preservative, fungal action, and particularly rich in anticancer effects.

The present invention relates to the technical field belonging to fertilizers, especially fertilizers containing amino acids, sugar acids and chelate compounds formed by fatty acids and metals.

Conventional Technology

Fertilizers are applied to the soil to feed the crops, increase yields, and prevent the decline of intellect.

Essential ingredients of fertilizer are the three main components of nitrogen (N), phosphorus (P), girly (K) which is necessary for the growth of plants and is required for mass supply.

Nitrogen promotes plant protein synthesis, cell division and proliferation, root growth, leaf growth, as well as nutrient absorption and assimilation.

Phosphorus (P) promotes the development of roots and increases germination by increasing the nutrient absorption surface from the roots.

It also promotes the maturation of plants, prevents pests and at the same time improves the yield. Girly (K) synthesizes carbohydrates and nitrogen compounds, regulates assimilation, root evaporation, and increases resistance to cold and pest.

Other fertilizers include calcium (Ca), magnesium (Mg), silicon (Si), manganese (Mn), zinc (Zn), copper (Cu), molybdenum (Mo), iron (Fe), boron (B), etc. The components of are blended.

For example, as shown in Japanese Unexamined Patent Publication No. 55-15901, urea, nitrogen and phosphorus are suppressed by adding pH which does not harm the growth of the crop while suppressing PH rise by hydrolysis of urea. A method for stabilizing a liquid fertilizer for preparing a liquid fertilizer containing, girly, magnesium and the like is already known.

Japanese Unexamined Patent Publication No. 58-223681 also discloses a liquid fertilizer in which a liquid fertilizer containing phosphoric acid, magnesium and calcium ions is added to prevent the precipitation of crystalline substances.

In addition, Japanese Patent Application Laid-Open No. 9-183681 discloses a chelate that combines a metal compound and a sugar acid to form a chirate compound to grow crops having good color, fragrance, and the like, and having good anticancer effects. A compound fertilizer is disclosed.

Problems to be Solved by the Invention

In the prior art as described above, since the chirate compound is formed only of a sugar acid and a metal, the stability of the chirate compound is low, and the physical properties of the chelate compound may be deteriorated before ingestion in vivo.

Originally, fertilizers do not contain harmful substances, contain large amounts of inactive ingredients, are easy to handle, and inexpensive.

In addition, in recent years, crops with high commodity value that meet consumer needs are required, and the quality of crops is improved and fertilizers are improved because the quality of texture, color, etc. is excellent, the flavor and taste are excellent, and the quality of consumer health-oriented drugs is required. You must do it.

In order to meet the needs of the times as described above, the present invention can cultivate crops having good color, aroma, taste, and antiseptic fungal action, and ingesting these crops detoxifies hydrogen peroxide, organic peroxide, cancer, etc. in the body. An object of the present invention is to provide a fertilizer containing a chilate compound that produces an enzyme that acts as an antioxidant.

In addition, an object of the present invention is to provide a fertilizer containing a chelate compound that can inhibit the degeneration of the chelate compound before ingestion in vivo.

Means to solve the problem

Chirate compound-containing fertilizer according to the present invention comprises 0.01 to 4 parts of the metal compound, 1 to 5 parts of the amino acid mixture, 2 to 8 parts of scallop, 0.1 to 1 part of nitrogen fertilizer, 0.01 to 2 parts of phosphate fertilizer When mixed with, the pH becomes 6.0 to 7.5.

Amino acid mixtures are sugar acids, fatty acids or sugar acids and mixtures of fatty acids with amino acids.

Chilate compounds formed by metals and mixtures of one or both of sugar acids and fatty acids in amino acids are absorbed into crops along with other fertilizer components when applied to soil as fertilizer.

When these crops are ingested by animals such as humans, the chelate compound is linked to peptides present in the body and becomes an active center that reduces free radicals and oxides by the substrate. (GST) molecular species and detoxifying enzymes such as guruthione peroxidase (GP _x ) are induced.

Electrophilic compounds present in the living body are substances that react with electrons of a counter molecule by sharing electrons with a counter molecule by attacking a high electron density place of a counter molecule by a spontaneous reaction such as a sphere electron substitution reaction or a sphere electron addition reaction. to be.

For example, electrophilic compounds attack high-density sites of metal-nitrogen bonds (eg, Si-N bonds, Al-N bonds, BN bonds, etc.) of metal-containing polymers to separate these bonds to form new bonds. It has an electrophilic group to produce.

Such electrophilic compounds are produced by hydroxylation of carcinogens containing at least some of polynuclear aromatic hydrocarbons such as benzopyrene, azo compounds, aromatic amines, and many other chemicals, and are shared with the nucleophilic portion of DNA or proteins. In combination to express mutations, cytotoxicity and cancer.

The detoxification enzyme described above acts as a catalyst for the reduction of electrophilic compounds rich in reactive oxygen, hydrogen peroxide and organic peroxides with water or alcohols.

When an amino acid mixture in which one or both of a sugar acid and a fatty acid is added to an amino acid and a metal are mixed together, the formation of a metal chelate compound is easily performed since a large number of functional groups having a chelating ability such as an amino group and a carboxyl group are included. Particularly, amino acids are generally bidentate complexes by carboxyl groups and amino groups contained in one molecule of amino acid when the nitrogen atom of amino group has strong electron donor and forms a chelate compound with metal as electron acceptor. ) To form a 5-membered ring.

Complexes having a chelated ring are relatively safer than comparable simple complexes, and the 5-membered ring has the safest chelating effect among the chelating compounds. Precipitation of the metal compound does not occur and does not deteriorate. In addition, even when sprayed as a fertilizer in the soil, it is hardly decomposed by microorganisms, so that the chelating compounds in the fertilizer are reliably absorbed by the crops, and when the crop is ingested in vivo, it acts as a catalyst to reduce the active oxygen as described above. Enzymes are produced in high yield.

When the metal compound is less than 0.01 parts by weight, no chirate compound is formed. When the metal compound is more than 4 parts, the effect as a fertilizer containing the chilate compound does not change, but it is not good because the excess metal compound accumulates in the soil. When the total amount of one or both of the sugar acids and fatty acids added together to the amino acid is less than 1 part, the chilate compound is not formed and the effect does not change even after 5 parts.

When the content of scallop fertilizer is 2 to 8 parts, it synthesizes carbohydrates and nitrogen compounds and promotes assimilation, root development, flowering and fruiting.

If it is less than 2 parts, carbohydrates or nitrogen compounds are not sufficiently synthesized.

When the content of nitrogen fertilizer is 0.1 to 1 part, it promotes plant protein synthesis, cell division and proliferation, root development and leaf propagation. It also promotes the absorption and assimilation of nutrients. Can't. When the content of phosphate fertilizer is less than 0.01 part, the roots of crops are not sufficiently developed. If scallop, nitrogen fertilizer and phosphate fertilizer exceed 8 parts, 1 part and 2 parts, respectively, the physiological function of each fertilizer remains unchanged, but excess fertilizer remains in the soil and adversely affects it.

In addition, when the pH of the fertilizer containing fertilizer is less than 6.0, excessive soil contamination is concerned, and when it is more than 7.5, it affects the safety of the chelate complex.

Embodiment of the Invention

EMBODIMENT OF THE INVENTION Below, embodiment of the chirate compound containing fertilizer which concerns on this invention is described.

The fertilizer-containing fertilizer according to the present invention comprises 0.01-4 parts of metal compound, 1-5 parts of amino acid mixture, 2-8 parts of scallop, 0.1-1 part of nitrogen fertilizer and 0.01-2 parts of phosphate fertilizer. PH is 6.0 ~ 7.5 when mixed with.

The metal compound is one or two or more of sulfate, nitrate, phosphate, vinegar salt, carbonate, silicate, borate, chloride, oxide and hydroxide.

The metal of the metal compound forms the core metal of the chilate compound and is composed of calcium (Ca), magnesium (Mg), manganese (Mn), zinc (Zn), copper (Cu), molybdenum (Mo), iron (Fe), and aluminum. (Al), nickel (Ni), cobalt (Co), titanium (Ti), gallium (Ga), selenium (Se), germanium (Ge), vanadium (V), chromium (Cr), silver (Ag), straw One or two or more of lithium (Sr), indium (In), tin (Sn), gold (Au), and zirconium (Zr).

Especially as a metal compound, a selenium (Se) compound is preferable. Selenium is a component of guruthion peroxytase (GPx), an enzyme that reduces electrophilic compounds such as active oxygen to water or alcohols, and is connected to peptides and subjected to redox by substrates. It becomes active center.

Therefore, the fertilizer according to the present invention containing a serenium compound can rapidly induce guruthione peroxidase (GPx) to suppress mutant cells and carcinogenesis.

The amino acids of the amino acid mixture are one kind or two or more kinds of glycine, alanine, borin, arginine, lysine, aspartic acid, gurutamic acid, serine, sleonine, chirosine, methionine and cischin.

In embodiment in this invention, a sugar acid, a fatty acid, and a scouring waste liquid are used as an amino acid mixture. Silk refining fluid is used to dissolve and remove sericin on the colloid surrounding the whibroin component in the silk fiber in heated water, an aqueous solution of soap, or a solution containing an enzyme to purify the silk made of the whibroin alone. It is the waste liquid discharged.

The silk refined liquid contains proteins such as fibroin and sericin, which are polymers and have many functional groups, in addition to amino acids such as glycine, aracin, and serine. Such a scouring liquid contains a large number of multivalent ligands containing a plurality of coordination groups, and forms two or more chelate compounds simultaneously when coordinating with a metal, resulting in the formation of a chilate ring compared to one chelate ring. The effect increases synergistically to form more stable chirate compounds. Therefore, the chirate compound-containing fertilizer produced using the silk refining liquid does not denature even if left for a long time. In addition, sprayed in the soil is hardly decomposed by the microorganisms, so the chelating compound is surely absorbed by the crops.

As the sugar acid, pental acid, hexane acid (including gluconic acid) and the like are used. Fatty acids include saturated fatty acids such as formic acid, vinegar acid, propionic acid, lauric acid, myristic acid, palmitic acid and stearic acid, and unsaturated fatty acids such as acrylic acid, crotonic acid, oleic acid, sorbic acid, linoleic acid and linolenic acid.

The point fertilizer chloride is cerium, cerium hwangsanga, - and light _{(Kcl-Mgcl 2 -6H 2 0} ), fertilizers Kariya ore (containing kcl or K ₂ SO _4), you point Kariya salt (Kcl (+ Nacl + MgSO ₄ )), at least one species is used in the herbivore (K ₂ CO, KHCO ₃ ), seaweed (Kcl.K ₂ SO ₄ ), cement dust (sulphate, carbonate, silicate) and furnace dust (carbonate, sulfate).

Nitrogen fertilizers include ammonium sulfate ((NN ₄ ) ₂ SO ₄ ), ammonium chloride (NH ₄ Cl), ammonium nitrate (NH ₄ NO ₃ ), ammonium phosphate ((NH ₄ ) ₂ HPO ₄ , NH ₄ H ₂ PO ₄ ), Ammonia daitan (ammonium of organic acid), ammonia perlinic acid lime (NH ₄ H ₂ PO ₄ , (NH ₄ ) ₂ SO ₄ ), ammonium sulphate ((NH ₄ ) ₂ SO ₄ + NH ₄ NO ₃ ), Ammohose ((NH ₄ ) ₂ SO ₄ + (NH ₄ ) HPO ₄ ), Ammonium Sulfate ((NH ₄ ) ₂ SO ₄ + (NH ₄ ) ₂ HPO ₄ ), Harvey (Ammonium Salt), and Cattle Fertilizer ( Ammonium salt fertilizers such as ammonium salts), nitrile salt (NH ₄ NO ₃ ), lime nitrate (Ca (NO ₃ ) ₂ ), nitrate (KNO ₃ ), ammonium nitrate (NH ₄ NO ₃ + CaCO ₃ ), knife urea _{(Ca (NO 3) 2.} 4CO (NH 2) 2), such as nitrate, lime nitrogen (CaCN _2), cyan amide nitrogen such as, urea (CO (NH ₂₎ and knife urea (Ca ( _{NO 3) 2. 4CO (NH} 2) 2) of urea nitrogen, urea fertilizer branching coefficient (Ura _{hworeum) (CO (NH 2) 2} -HCHO condensate, oxazolyl polyimide (NH ₂ CO.CONH ₂₎ elements not obtain Salt (NH _2. ((NH). NH.CO.NH ₂ . X), amino-based nitrogen and fish oil (containing NH ₂ NH) of guanidine salt ((NH ₂ .C (NH) .NH ₂ .X) and peat ammonia (CO (NH ₂ ) ₂ )), and soybean meal (NH ₂ .NH content) is used at least one type from a nitrogen-based protein such as (X:., such as _{Hcl, H 2 SO 4, HNO} 3, H 3 PO 4)

Phosphate fertilizers include superphosphate lime (Ca (H ₂ PO ₄ ) 2+ CaSO ₄ ), superphosphate lime (Ca (H2PO ₄ ) ₂ ), gotophosphoric acid (hyperphosphate + chalcopyrite), soluble phosphorus ratio (CaO-MgO-P2O5-SiO ₂ Caras), small phosphorus ratio (Ca ₃ (PO ₄ ) ₂ -CaNaPO ₄ solid solution), mixed phosphorus ratio (hyperphosphate lime + soluble phosphorus ratio) phosphate powder fertilizer (Ca ₃ (PO ₄ ) ₂ ), precipitated phosphate lime (CaHPO ₄ ), Chlorinated ammonium sulfate ((NH ₄ ) ₂ SO ₄ + NH ₄ H ₂ PO ₄ + K ₂ SO ₄ ), solid fertilizer (ammonium sulfate + lime phosphate + gall salt + peat), phosphate form (Ca (H ₂ PO ₄ ) ₂ ) and at least one species of chemical fertilizers ((CaHPO ₄ , Ca ₂ (PO ₄ ) _2, etc.)).

Vegetables cultivated by fertilizing the fertilizer-containing fertilizer, since the fertilizer-containing fertilizer according to the present invention contains a chelate compound formed by amino acids, sugar acids and fatty acids and metals in a complex form that is easily absorbed by the metal in vivo. The crops are not only good in color, aroma, taste, etc., but also have an antiseptic and fungal effect, and can be stored for a long time.In addition, when they are eaten in vivo, detoxification effects such as active oxygen, hydrogen peroxide, organic peroxide, cancer, etc. Enzymes with antioxidant activity are produced.

EXAMPLE

Dissolve 0.07 parts of selenium trioxide (SeO3) in 3 parts of water by weight, add 1 part of amino acid, 1 part of pentalic acid, and 1 part of vinegar acid, extracted from silk refinement, and add 60 parts of water, and stir for 60 minutes. Dissolved. A point in the following fertilizers chomokhoe _{(re) (K 2 CO 3. KHCO} 3) was added 4 parts were stirred for 60 minutes. A nitrogen-containing fertilizer containing 0.5 parts of ammonium sulfate and 1 part of superphosphate lime (Ca (H ₂ PO ₄ ) ₂ + CaSO ₄ ) as a phosphate fertilizer was added to adjust the pH to 6.6.

The fertilizer containing the chelate compound prepared by the method described above was diluted with 10 times of water and sprayed 100cc once a week for 2 months on the roots of Altari radish. In order to compare fermented fermented fermented fermented fermented fertilizer containing fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented with fermented fermented fermented fermented with fermented fermented fertilizer. The amount of enzymes having antiseptic, antifungal and antifungal activity, cancer detoxification and antioxidant activity was investigated.

(1) The color, aroma, taste, etc. of the Altari radish were tested as it was or as it was fried at high temperature with oil.

(2) Altari radish was cut into small pieces and placed in a container containing water to be tested for antiseptic and mold activity under standing.

(3) Altari radish was fed to F 344 male rats (7 weeks old) for 3 weeks, and then liver and kidney were extracted and enzymes having detoxification and antioxidant activities such as cancer contained in these organs, for example, gurutathione S-trans Ferase (GST) molecular species and gurutathione peroxytase (GPx) were measured.

The measurement method of guruthion S-transferase (GST) molecular species is as follows. Three times the weight of 0.25M Scro-S-10mM phosphate buffer (PH7.4) was added to the isolated liver and kidney for homogenization. 50 ml of homogenized organ samples, 20 ml of 20% SDS and 275 ml of PBS were added, diluted 10-fold with water, and the total protein mass was measured by the Lowry method. The sample concentration was adjusted to a protein concentration of 1 mg / ml, boiled with boiling water for 2 minutes, and 50 ml of 0.1% BPB was added to adjust the electrophoretic sample. Using a 12.5% SDS-polyacrylamide gel (electrophoresis SDS-PAGE), the stocking die was electrophoresed at 36 mA for 1 hour until the stocking die passed through the stocking die at 28 mA.

BIO RAD Co., Ltd. was used for 2 hours at 80V using a TRANS-BLOT CELL, and the protein isolated by electrophoresis was transferred to a western blot.

The nitrocellulose membrane was appendixed with 3% Schememilk TBS buffer (PH7.4) for 1 hour.

rTySTAl (Ya), rGSTA3 (Yc), rGSTA4 (Yk), rGSTM1 (Yb1), rGSTM2 (Yb2), rGSTP1 (Yp), for BioTrin, International, Limited (BIOTRIN INTERNATIONAL Limited) The Krona antibody was washed twice with TBS buffer containing 0.05% Tween 20 for 5 minutes with TBS buffer containing 3% bovine albumin (BSA) and again with primary TBS buffer for 5 minutes.

Alkaline phosphatase-labeled anti-toki 1g G goat antibody from Jackson Imuno Reserch Laboratoris Inc was diluted 5000 times with TBS buffer (PH7.4) and reacted at room temperature for 1 hour. The secondary antibody was washed like the primary antibody.

Nitrobula Tetra (Nitro Blue Tetra) manufactured by Pierce as a color development reagent was subjected to color reaction and immunostaining (Immunostaining).

The color bands of each sample were placed in an ad photoshop of Photoretouch Soft, and the intensity of the color bands was measured using an NIH image analyzer.

By comparing the results obtained, the induction of the GST molecular species obtained by the significant difference test (t-test) was investigated.

The measured values of the GST molecular species were determined by setting the intensity of the color band for the liver and kidney of F344 male rat (rat) (7 weeks old) fed with a conventional commercial solid feed for 3 weeks instead of alta radish to 1 (no unit). ) Was calculated.

As shown in the test results of Table 1, the color, aroma, taste, preservation and anticorrosion in the examples of Altari radish containing selenium (Se) compared to the comparative example of Altari radish cultivated without fertilizing the chilate compound-containing fertilizer as shown in the test results of Table 1 Significant effects were shown on fungal action. In addition, in the amount of enzymes having detoxification and antioxidant activity such as cancer, guruthione S-transferase (GST) molecular species showed significant differences with respect to the examples of kidney and liver.

Gurutathione peroxidase (GPx) showed a significant difference in kidney. As a result, it was found that the crops fertilized with the fertilizer-containing fertilizer in the present embodiment produced enzymes having detoxification and antioxidant effects such as cancer when ingested.

.

As described above, crops grown by fertilizing fertilizers containing chirate compounds according to the present invention have good color, aroma, and taste, and have antiseptic and mildew effects, so that even if they are kept fresh for a long time and transported to remote sites, It does not deteriorate.

In addition, when ingested in vivo, it causes enzymes having detoxification and antioxidant activity, such as cancer, thereby improving health.

Claims (7)

0.01 to 4 parts by weight of metal compound, 1 to 5 parts of amino acid mixture, 2 to 8 parts of scallop fertilizer, 0.1 to 1 part of nitrogen fertilizer, and 0.01 to 2 parts of phosphate fertilizer. PH of 6.0 to 7.5 when mixed with water Fertilizer containing a chilate compound, characterized by consisting of sugar acids, fatty acids or mixtures of sugar acids and fatty acids with amino acids The amino acid is one or two or more of glycine, alanine, borin, arginine, lysine, aspartic acid, gurtamic acid, serine, sleonine, tyrosine, methionine and cysten, and the fertilizer containing a chelating compound according to claim 1 The amino acid mixture is a fertilizer containing a chilate compound according to claim 1 or 2, which is a silk refined liquid. The metal compound is a fertilizer containing the chilate compound according to claim 1, which is one or two or more of sulfate, nitrate, phosphate, vinegar, carbonate, silicate, borate, chloride, oxide, and hydroxide. Metals constituting the metal compound include calcium, magnesium, manganese, zinc, copper, molybdenum, iron, aluminum, nickel, cobalt, titanium, garium, selenium, germanium, vanadium, chromium, silver, strontium, indium, tin, gold And fertilizers containing chilate compounds according to claim 1, which are one or two or more of zirconium. The metal compound is a selenium compound, the fertilizer containing a chilate compound according to claim 1 Selenium trioxide 0.03--2 parts, amino acids 0.5-1.5 parts, pentanic acid 0.5-1.5 parts, vinegar 0.5-1.5 parts, scallop fertilizer 3-5 parts, nitrogen fertilizer 0.3-0.8 parts, phosphate fertilizer 0.5-1 Hydrogen ion concentration when mixed with water, including parts, is the fertilizer containing the chilate compound as described in claim 1, which is PH 6.3 to 7.0.