JP2002171855A - Agent and method for treating disease damage to cultured laver and exterminating miscellaneous algae - Google Patents

Abstract

PROBLEM TO BE SOLVED: To effectively get rid of disease damages and to exterminate miscellaneous algae without damaging laver. SOLUTION: Laver or an aquaculture tool such as a laver net stuck with laver is in contact with a treatment solution which contains 0.001 wt.% to 0.01 wt.% of salicylic acid and is adjusted to pH 1.5-4 for 1-20 minutes.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to a treatment agent for controlling the disease of cultured seaweed and the algae, and more particularly to a diatom and other algae, a red rot fungus, a white rot fungus, a pot fungus and the like. The present invention relates to a treatment agent for controlling diseases.

[0002]

2. Description of the Related Art Conventionally, as a treatment method and a treatment liquid for seaweed for exterminating the disease and algae of cultured seaweed, for example, Japanese Patent Publication No. 56-12601, Japanese Patent Publication No. 60-31451, and Japanese Patent Publication No. There is one disclosed in, for example, Japanese Patent No. 31647. All of these prior arts perform an acid treatment using various organic acids or inorganic acids for the purpose of controlling and controlling algae and diseases attached to laver. The invention disclosed in Japanese Patent Application Laid-Open No. 9-201180 is a prior application of the present applicant, in which the specific gravity of seawater was adjusted using an inorganic salt such as sodium chloride or magnesium chloride and an acid. The treatment is performed using a treatment liquid. The conventional method for treating seaweed using the above-mentioned acid has achieved to some extent the purpose of controlling or exterminating algae and diseases attached to the seaweed. However, these conventional acid treatment techniques alone may not always be sufficient. For example, when a large amount of diatoms of the type that grow on the surface of laver adhere to the surface of the laver, they appear in the laver product as green spots generally called "hato feces". However, the conventional acid treatment as described above cannot always be expected to have a sufficient effect on diatoms that affect such products, and particularly, a short-time treatment such as treatment with a mogul boat or through treatment. Or in cold regions with low seawater temperatures, normal acid treatment may not be able to provide a sufficient effect. Therefore,
If the disease or damage from the algae is large, it is necessary to use a large amount of acid, but the use of a large amount of acid may damage the laver.

[0003]

DISCLOSURE OF THE INVENTION The present invention has been made in view of the above-mentioned current state of diseases and algae control in cultured seaweed, and effectively eliminates diseases and algae in cultured seaweed without damaging the seaweed. It is intended to provide a treating agent and a treating method.

[0004]

Means for Solving the Problems The present inventors have conducted intensive studies to achieve the above object, and as a result, by using salicylic acid in combination with acid treatment, it is possible to effectively reduce the disease of cultured seaweed and diatoms such as diatoms. The inventors have found that they can be exterminated and that they damage the laver little, and have completed the present invention.

[0005] That is, the treatment agent of the present invention for controlling the disease of cultured seaweed and seaweeds contains salicylic acid in an amount of 0.0005% by weight or less.
It contains 0.1% by weight and has a pH adjusted to a range of 1 to 4. Furthermore, the combined use of lactic acid and acetic acid improves the disease and harmful algal control effects. In addition, salicylic acid sodium salt or salicylic acid ester can be used instead of salicylic acid.

[0006] The treatment agent for controlling the disease of cultivated laver and the algae according to the present invention as described above exerts a sufficient control effect on the adhered diatom, which is the main cause of the above-mentioned "pigtails" appearing in the laver products. . Further, since a sufficient control effect can be exerted even when the pH of the treatment liquid at the time of use is about 2.0, there is little possibility of damaging the seaweed.

In the treating agent of the present invention, propylene glycol, ethylene glycol, butanediol, polyethylene glycol, polypropylene glycol, polybutylene glycol, ethyl alcohol, methyl alcohol, isopropyl alcohol and the like are blended as a dissolution aid for salicylic acid. be able to.

The treating agent of the present invention may contain an organic acid and an inorganic acid other than lactic acid and acetic acid as a pH adjuster.

[0009] In a preferred method of treating cultured seaweed,
A culture tool such as laver or laver net with laver is contacted for 1 to 20 minutes with a treatment liquid containing salicylic acid in an amount of 0.001% to 0.01% by weight and a pH adjusted to 1.5 to 4. Let it.

In another preferred method of treating cultured seaweed, salicylic acid is contained in an amount of 0.005% by weight to 0.05% by weight,
A culture tool such as laver or a laver net to which laver is adhered is brought into contact with the treatment solution whose pH has been adjusted to a range of 1 to 2.5 for 30 seconds to 2 minutes.

In still another preferred method of treating cultured seaweed, seaweed or seaweed is added to a processing solution containing salicylic acid in an amount of 0.01% by weight to 0.07% by weight and a pH adjusted to a range of 1 to 2.5. 10 seconds to 3 days for culturing tools such as laver nets
Contact for 0 seconds.

[0012]

BEST MODE FOR CARRYING OUT THE INVENTION The treatment agent for controlling the disease and algae of cultured laver according to the present invention is obtained by dissolving salicylic acid, lactic acid, acetic acid, and inorganic or organic acids for adjusting pH in seawater or the like. It is prepared as a liquid. Salicylic acid blended in the treatment solution has low solubility in water, and its solubility is improved by using it in combination with other acids such as lactic acid and acetic acid.However, when the concentration of lactic acid or acetic acid is low, the solubility of salicylic acid is low. May be bad. In such a case, as a dissolution aid,
Propylene glycol, ethylene glycol, butanediol, polyethylene glycol, polypropylene glycol, polybutylene glycol, ethyl alcohol,
It is preferable to mix methyl alcohol and isopropyl alcohol. In this case, in preparing the treatment liquid, it is preferable that salicylic acid is dissolved in the dissolution aid such as acetic acid or propylene glycol, and then mixed with other components.

In the present invention, salicylic acid esters such as sodium salicylate or ethyl salicylate, methyl salicylate, isobutyl salicylate or n-butyl salicylate can be used in place of salicylic acid. In the case of the sodium salicylate, it is soluble in water but not in acid. Therefore, when preparing a treatment liquid, it is preferable to add an acid to water in which sodium salicylate is previously dissolved and mix. In addition,
In addition to the lactic acid and acetic acid, pyruvic acid, propionic acid,
Even when formic acid or the like is used in combination with salicylic acid, the same effects as those of lactic acid and acetic acid can be expected to eliminate the disease of cultured seaweed and the control of algae.

The method for treating seaweed with the treatment agent for controlling diseases and algae according to the present invention as described above is not particularly limited. For example, in a method called immersion treatment, the treatment liquid is transferred to a container such as a treatment liquid tank in a ship. The seaweed or seaweed to which the seaweed is attached is immersed in the treatment liquid to bring the seaweed into contact with the treatment liquid. The concentration of the treatment liquid used in the case of treatment for a relatively long time such as this immersion treatment is such that salicylic acid is 0.0005% by weight to 0.03%.
% By weight, preferably 0.001% to 0.01% by weight
It is preferable to adjust the density to a relatively low density so as to fall within the range of When used in combination with lactic acid or acetic acid, the concentration of lactic acid or acetic acid to be used is in the range of 0.01% by weight to 0.1% by weight, preferably 0.03% by weight to 0.1% by weight. Is 0.001 to 0.01% by weight, preferably 0.001 to 0.005% by weight.
Range. In addition, the immersion time of the nori in the treatment liquid is determined by the growth state of the nori, the status of diatoms and other weeds and diseases, the type of acid used in the treatment agent, their concentration, the pH of the treatment liquid, and the temperature during treatment. Depending on the case, it is usually 1-2
It may be within 0 minutes.

[0015] In a relatively short time, a ship is buried under a nori cultivation net, such as a mogul boat, and passed through the net while passing the processing liquid through the net. Processing can also be performed. In the case of the short-time treatment by the mogul boat, it is preferable to set the concentration of the treatment liquid to be higher than that of the immersion treatment.
Wt% to 0.1 wt%, preferably 0.005 wt% to
0.04% by weight. When used in combination with lactic acid or acetic acid, the concentration of the lactic acid or acetic acid used in combination is 0.1% by weight to 1% by weight.
0% by weight, preferably in the range of 0.2% by weight to 1.0% by weight, and the concentration of salicylic acid is 0.01% by weight to 0.05% by weight.
% By weight, more preferably in the range of 0.01% to 0.03% by weight. The processing time by this mogul boat is 3
It is about 0 seconds to 2 minutes, usually about 40 seconds to 1 minute.

Further, in some areas, there is a method called a through treatment, in which a treatment liquid is stored in a container such as a treatment liquid tank in a small ship, and the ship is sunk under a nori cultivation net. This is a treatment method in which the seaweed is brought into contact with the treatment liquid by passing the seaweed culture net while immersing the same in the liquid. In this case, it is preferable to further increase the concentration of the treatment liquid, and the salicylic acid content is preferably 0.005% to 0.1% by weight, more preferably 0.01% to 0.07% by weight. When used in combination with lactic acid or acetic acid, the concentration of lactic acid or acetic acid used in combination is 0.1% by weight to 1.0% by weight, preferably
Weight% to 1.0 weight%, and the concentration of salicylic acid is 0.01 to 0.1 weight%, preferably 0.01 to 0.0 weight%.
It is in the range of 5% by weight. The processing time of this transparent processing is usually about 10 to 30 seconds.

The above processing solution can be easily adjusted to a desired pH by using hydrochloric acid, phosphoric acid or the like. P
Examples of the acid used for adjusting H include inorganic acids, such as sulfuric acid and nitric acid, in addition to the above hydrochloric acid and phosphoric acid. Examples of the organic acid include malic acid, citric acid, fumaric acid, gluconic acid, maleic acid, malonic acid, formic acid, tartaric acid, acrylic acid, crotonic acid, oxalic acid, succinic acid, and glutaric acid. Further, phytic acid can be used as the organic phosphoric acid, and metaphosphoric acid, polyphosphoric acid, and the like can be used as the polymerized phosphoric acid. These acids can be used alone or in combination of two or more. The amount of these acids used is 1.5 when the pH of the processing solution is relatively long, such as immersion.
In the case of processing in a relatively short time, such as processing within a range of ~ 4, and processing on a moat boat or through-through processing, it is preferable to adjust the amount to a range of 1 ~ 2.5.

Further, nutrients can be added to the treatment liquid of the present invention, if necessary. The added nutrients include ammonium salts such as ammonium chloride, ammonium nitrate, ammonium phosphate and ammonium sulfate; sodium salts such as sodium nitrate, sodium phosphate and sodium sulfate; potassium salts such as potassium nitrate, potassium phosphate and potassium sulfate; Examples include amino acids such as glycine, glutamic acid, and lysine, and decomposed solutions of plant protein decomposed products and animal protein decomposed products. These nutrients can be added and mixed at the same time as salicylic acid and another acid such as lactic acid or acetic acid, or may be added to any of them in advance. Furthermore, you may make it add at the time of dilution with seawater.

[0019]

EXAMPLES [Experimental Example 1] The treating agent components shown in Table 1 below were added to artificial seawater and mixed to prepare a treatment liquid for laver in each test section. The pH of the treatment liquid was adjusted to 2.0 with hydrochloric acid. After immersing the chlorinated vinyl chloride plate in these treatment solutions for 5 minutes and taking it out, it was washed with the same artificial seawater as described above (about 20 seconds), and then returned to fresh artificial seawater.
The extermination effect of diatoms adhering to the vinyl chloride plate was determined. Determination of the diatom control effect indicates the diatom staining rate by erythrosine staining in the range of “−” to “100%”,
"-" Has no effect, and the higher the "%", the more effective. In addition, the influence on the nori leaf was determined based on the staining rate of the nori leaf by erythrosine staining and the degree of damage to the buds observed by microscopy. The determination of the staining rate of the laver leaf and the degree of damage to the buds are as follows. That is,
The staining rate of the nori leaf body is shown in the range of "-" to "+++",
"-" Was not considered to be damaged at all, and the more the number of "+", the worse the damage. In addition, about the damage condition of the bud,
“○; no damage”, “△; slightly damaged”, and “×: damaged”. In addition, a commercially available acid treating agent (W35
0; Fuso Chemical Co., Ltd.) and hydrochloric acid only. The results are shown in Table 1.

[0020]

[Table 1]

As is clear from the results in Table 1, a high diatom control effect was obtained by immersing seaweed in salicylic acid, or a treatment solution in which lactic acid was used in combination with salicylic acid. In addition, since the bud damage of nori caused by acid treatment tends to show a relatively large difference depending on the size of each nori at each growth stage, the degree of soundness at the time of treatment, and furthermore, depending on the cultivation area, the evaluation of the degree of damage of the bud is described above. The results are relative.

Experimental Example 2 The treating agent components shown in Table 2 below were added to artificial seawater and mixed to prepare a treatment liquid for laver in each test section. The pH of the treatment liquid was adjusted to 2.0 with hydrochloric acid. The chlorinated vinyl chloride plate was immersed in these treatment liquids for 10 seconds, taken out, allowed to stand for 50 seconds (1 minute in total), and then washed with artificial seawater as described above (about 20 minutes).
Seconds) and then returned to fresh artificial seawater, and the effect of removing diatoms adhering to the vinyl chloride plate was examined. In addition, the effect on the laver leaf was determined in the same manner as in Experimental Example 1. The results are shown in Table 2.

[0023]

[Table 2]

Experimental Example 2 is a treatment in a processing time of 1 minute assuming treatment by a mogul boat. As is clear from the results in Table 2, salicylic acid, and a treatment agent using lactic acid or acetic acid in combination with salicylic acid was used. Also, diatoms can be effectively eliminated by short-time treatment similar to the treatment using a mogul boat.

[Experimental Example 3] The treating agent components shown in Table 3 below were added to artificial seawater and mixed to prepare a treatment liquid for laver in each test section. The pH of the treatment liquid was adjusted with hydrochloric acid. The chlorinated vinyl chloride plate was immersed in these treatment solutions for 5 seconds, taken out, and left for 10 seconds (total 15 seconds).
Thereafter, after washing with the same artificial seawater as described above (about 20 seconds), it was returned to new artificial seawater, and the diatom control effect was examined as in Experimental Example 1. In addition, the effect on the laver leaf was determined in the same manner as in Experimental Example 1 based on the staining rate of the laver leaf by erythrosine staining and the degree of damage to the buds observed by microscopy. Also,
Commercially available acid treatment agent (W Dash; Fuso Chemical Industry Co., Ltd.)
A similar experiment was performed for The results are shown in Table 3.

[0026]

[Table 3]

Experimental Example 3 was a treatment with a treatment time of 15 seconds assuming a through treatment. As is clear from the results in Table 3, salicylic acid, and a treating agent in which salicylic acid and lactic acid were used in combination, used a mogul boat. Diatoms can be effectively eliminated by short-time treatment similar to the treatment used.

Experimental Example 4 After dissolving sodium salicylate in artificial seawater to a concentration shown in Table 4 below, commercially available acid treatment agents (W-dash, W-clean FX; both manufactured by Fuso Chemical Industry Co., Ltd.) were used. Add, mix, and add 1
A 100-fold dilution was prepared. After immersing the chlorinated vinyl chloride plate in these treatment liquids for 10 seconds and taking them out, they were left for 50 seconds (total 1 minute), and then washed with artificial seawater as described above (about 20 seconds). Then, it was returned to fresh artificial seawater, and the diatom control effect was examined in the same manner as in Experimental Example 1.
In addition, the effect on the laver leaf was determined in the same manner as in Experimental Example 1. The results are shown in Table 4.

[0029]

[Table 4]

[Experimental Example 5] After dissolving sodium salicylate in artificial seawater to the concentration shown in Table 5 below, a commercially available acid treating agent (W350, W700; both are Fuso Chemical Industries, Ltd.) was added and mixed. Thus, a 100-fold dilution of each test plot was prepared. After immersing the vinyl chloride plate on which the diatoms had adhered in these treatment liquids for 5 minutes and taking them out, they were washed with the same artificial seawater as described above (about 20 seconds), and then returned to fresh artificial seawater. The diatom control effect was examined in the same manner as described above. In addition, the effect on the laver leaf was determined in the same manner as in Experimental Example 1. Table 5 shows the results.

[0031]

[Table 5]

As is clear from the results in Tables 4 and 5, diatoms can be effectively controlled by using sodium salicylate in combination with a conventional acid treatment agent.

The acid treatment agents used in Examples 1, 3, 4 and 5 are as shown in Table 6.

[0034]

[Table 6]

[Experimental Example 6] The treating agent components shown in Table 7 below were added to artificial seawater and mixed to prepare a treatment liquid for laver in each test section. The pH of the treatment liquid was adjusted with hydrochloric acid. After immersing the chlorinated vinyl chloride plate in these treatment liquids for 10 seconds and taking them out, they were left for 50 seconds (total 1 minute),
Thereafter, after washing with the same artificial seawater as described above (about 20 seconds), it was returned to new artificial seawater, and the diatom control effect was examined as in Experimental Example 1. In addition, the effect on the laver leaf was determined in the same manner as in Experimental Example 1. The results are shown in Table 7.

[0036]

[Table 7]

As is clear from the results in Table 7, diatoms can be effectively controlled by using lactic acid and salicylic acid ester in combination.

[Experimental Example 7] The treatment agent components shown in Table 8 below were added to artificial seawater and mixed to prepare treatment liquid for laver in each test section. The pH of the treatment liquid was adjusted with hydrochloric acid. The diatom-adhered vinyl chloride plate was immersed in these treatment liquids (10 ° C.) for 10 seconds, taken out, allowed to stand for 50 seconds (total 1 minute), and then washed with the same artificial seawater as above (about 2 minutes).
(0 seconds), and then returned to fresh artificial seawater, and the diatom control effect was examined as in Experimental Example 1. Further, the effect on the laver leaf was determined in the same manner as in Experimental Example 1. The results are shown in Table 8.

[0039]

[Table 8]

As is evident from the results in Table 8, when lactic acid or acetic acid and salicylic acid are used in combination, the diatom control effect is equal to or greater than that when lactic acid or acetic acid is used in combination with caprylic acid or paraoxybenzoate. As well as no seaweed damage.

[Examples] Treatment preparations for controlling the disease of cultured seaweed and exterminating algae as shown in the following formulation examples 1 to 7 were prepared. After diluting a vinyl chloride plate with diatoms for 10 seconds into a treatment solution (10 ° C. or 15 ° C.) prepared by diluting the treatment formulation of each formulation 100-fold with seawater, and taking out,
It was left for 50 seconds (total 1 minute), washed with the same artificial seawater as described above (about 20 seconds), returned to fresh artificial seawater, and examined for diatom control effect in the same manner as in Experimental Example 1. In addition, the effect on the laver leaf was determined in the same manner as in Experimental Example 1. The results are shown in Tables 9 and 10.

(Formulation Example 1) 40% lactic acid (80% solution)
Acetic acid (90% solution) 20%, phosphoric acid (75% solution) 10
%, Hydrochloric acid (35%), 12% salicylic acid, 1% salicylic acid and 17% propylene glycol, dissolve salicylic acid in a mixture of acetic acid and propylene glycol, add lactic acid, phosphoric acid and hydrochloric acid and mix uniformly. A treatment formulation was prepared.

(Formulation Example 2) 50% lactic acid (80% solution)
Acetic acid (90% solution) 15%, phosphoric acid (75% solution) 10
%, Hydrochloric acid (35%), salicylic acid 1%, and propylene glycol 12%, dissolve salicylic acid in a mixture of acetic acid and propylene glycol, and add lactic acid, phosphoric acid and hydrochloric acid thereto, and mix them uniformly. A treatment formulation was prepared.

(Formulation Example 3) 60% of lactic acid (80% solution)
Acetic acid (90% solution) 15%, phosphoric acid (75% solution) 10
%, 6% hydrochloric acid (35%), 1% salicylic acid and 8% propylene glycol, dissolve salicylic acid in a mixture of acetic acid and propylene glycol, and add lactic acid, phosphoric acid and hydrochloric acid thereto, and mix them uniformly. A treatment formulation was prepared.

(Formulation Example 4) 60% lactic acid (80% solution)
Acetic acid (90% solution) 20%, phosphoric acid (75% solution) 10
%, 6% hydrochloric acid (35% solution), 1% salicylic acid and 3% propylene glycol, dissolve salicylic acid in a mixture of acetic acid and propylene glycol, and add lactic acid, phosphoric acid and hydrochloric acid thereto and mix them uniformly. To prepare a preparation for treatment.

(Formulation Example 5) 40% of lactic acid (80% solution)
Acetic acid (90% solution) 20%, phosphoric acid (75% solution) 10
%, 6% hydrochloric acid (35% solution), 1.5% salicylic acid and 22.5% propylene glycol, dissolve salicylic acid in a mixture of acetic acid and propylene glycol, and add lactic acid, phosphoric acid and hydrochloric acid to this. A treatment formulation was prepared by mixing uniformly.

(Formulation Example 6) 50% of lactic acid (80% solution)
Acetic acid (90% solution) 20%, phosphoric acid (75% solution) 10
%, 6% hydrochloric acid (35%), 1.5% salicylic acid and 12.5% propylene glycol, dissolve salicylic acid in a mixture of acetic acid and propylene glycol, and add lactic acid, phosphoric acid and hydrochloric acid to the mixture. To prepare a preparation for treatment.

(Formulation Example 7) 40% of lactic acid (80% solution)
Acetic acid (90% solution) 15%, phosphoric acid (75% solution) 10
%, 6% hydrochloric acid (35%), 2% salicylic acid and 27% propylene glycol, dissolve salicylic acid in a mixture of acetic acid and propylene glycol, and add lactic acid, phosphoric acid and hydrochloric acid thereto and mix them uniformly. A treatment formulation was prepared.

[0049]

[Table 9]

[0050]

[Table 10]

[0051]

As described above, according to the present invention, it is possible to eliminate diatoms and other algae and diseases more effectively than conventional acid treatment without damaging the seaweed during the treatment of cultured seaweed. Can be.

Claims (8)

[Claims] (1) Salicylic acid is contained in an amount of 0.0005% by weight to 0.
An agent for controlling disease and algae of a cultured seaweed containing 1% by weight and adjusting the pH to a range of 1 to 4. 2. The treatment agent according to claim 1, which comprises at least one of lactic acid and acetic acid. 3. The method of claim 1 or 2, wherein sodium salicylate or salicylate is used in place of salicylic acid. 4. The dissolution aid for salicylic acid is at least selected from the group consisting of propylene glycol, ethylene glycol, butanediol, polyethylene glycol, polypropylene glycol, polybutylene glycol, ethyl alcohol, methyl alcohol and isopropyl alcohol. The disease-treating agent for cultured seaweed and the treatment for algae according to any one of claims 1 to 3, which comprises one kind. 5. The treatment agent for controlling disease and harmful alga of cultured seaweed according to any one of claims 1 to 4, wherein at least one of an organic acid and an inorganic acid other than lactic acid and acetic acid is blended as a pH adjuster. . 6. Salicylic acid is added in an amount of 0.001% by weight to 0.0% by weight.
A method for treating cultured seaweed, comprising contacting a seaweed or a seaweed tool such as seaweed net with seaweed for 1 to 20 minutes to a treatment solution containing 1% by weight and adjusting the pH to a range of 1.5 to 4. Method. 7. Salicylic acid is added in an amount of 0.005% by weight to 0.0%.
A culture solution containing 5% by weight and a pH adjusted to a range of 1 to 2.5 is brought into contact with a culture tool such as laver or a laver net to which laver is adhered for 30 seconds to 2 minutes. Processing method. 8. Salicylic acid is added in an amount of 0.01% by weight to 0.07% by weight.
A method for treating cultured seaweed, comprising contacting a culture solution, such as seaweed or a seaweed net with seaweed, for 10 seconds to 30 seconds with a treatment liquid containing 1 wt% and a pH of 1 to 2.5. Method.