JP2001322937A - Antiallergenic composition and method of inactivation for allergen - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an antiallergenic composition for inactivating allergens contained in the environment and provide a method of inactivation for allergen using the composition. SOLUTION: This antiallergenic composition is obtained by including a rare earth salt and the method of inactivation for allergens is carried out by using this composition.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to an anti-allergen composition for inactivating environmental allergens and a method for inactivating allergens using the same.

[0002]

2. Description of the Related Art Allergic diseases such as asthma and atopic dermatitis have been plagued by many people for many years. Causes of these allergic diseases (hereinafter referred to as allergens)
As typical examples, mite, pet hair, pollen, and the like living indoors are well known. At present, pharmacotherapy is mainly applied to the treatment of allergic patients, while removing allergens from the living environment of the patient and rational measures to protect patients directly from exposure to allergens are also possible. It is. Such symptom improvement by allergen removal has been reported in Europe and the United States in addition to Japan.

[0003] As a method of removing allergens, there are suction by an electric vacuum cleaner, removal by an air cleaner, use of a high-density cover for bedding, and the like. However, there is a limit to the amount of allergen that can be removed only by suction using a vacuum cleaner, and an air purifier can only remove allergens that fly in the air. In addition, in the case of a high-density cover of bedding, allergens are removed from the inside, but allergens are not removed from the outside, and these methods are not always satisfactory.

As a method for chemically inactivating allergens, a method using tannic acid is proposed in Japanese Patent Publication No. 2-16731, and a method using tea extract, gallic acid or the like is proposed in JP-A-6-279273. ing. However, in these methods, there is a problem that it is difficult to obtain a stable allergen inactivation effect, and furthermore, some coloring occurs on the processed object.

[0005] Acaricides are generally used to control mites in house dust. Dermatophagoides farinae and Dermatophagoides pteronyssinus in house dust have allergenic properties even after dying. As it breaks down, it gradually releases allergens in particulate form, so killing a tick alone does not inactivate the allergen.

[0006]

Means for Solving the Problems The present inventors have conducted intensive studies to solve such problems, and as a result, it has been found that a composition containing a rare earth salt does not cause coloring of a treated object. The present inventors have found that they have an allergen inactivating effect and that they can stably inactivate allergens in the environment by treating this composition in the environment, and have reached the present invention. That is, the present invention provides an anti-allergen composition containing a rare earth salt, and a method for inactivating the allergen by spraying the composition.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION The rare earth salts in the antiallergen composition of the present invention include scandium salts, yttrium salts, lanthanum salts, cerium salts, praseodymium salts, neodymium salts,
Samarium salts, europium salts, gadolinium salts, terbium salts, dysprosium salts, holmium salts, erbium salts, thulium salts, ytterbium salts and lutetium salts are preferred, with yttrium salts, lanthanum salts and cerium salts being particularly preferred. As the salt, any salt such as acetate, nitrate, chloride, bromide, iodide, carbonate, phosphate, and hydroxide may be used, but sulfate, chloride, and the like may be used. Is preferred. These rare earth salts can be used as they are, but are usually used after being held on a solid carrier or a liquid carrier. The content of rare earth salts is usually
0.01 to 50% by weight, more preferably 0.1 to 50% by weight.
5% by weight. The dosage form of the anti-allergen agent composition of the present invention may be in any form, such as liquid, powder, and paste, as long as the treatment can inactivate the allergen in the environment. It is easy to handle and effective. In order to carry out such a preparation, a solvent suitable for dissolving or dispersing the rare earth salt as an active ingredient can be used alone or as a mixed solvent of two or more kinds.
Although not particularly limited to the solvent, for example, water, methyl alcohol, ethyl alcohol, isopropyl alcohol, benzyl alcohol, acetic acid, acetone, dimethylformamide, dimethylacetamide, dimethylsulfoxide,
Ethylene glycol, propylene glycol, diethylene glycol, triethylene glycol, dipropylene glycol, hexylene glycol, polyethylene glycol, glycerin, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, diethylene glycol monobutyl ether, diethylene glycol Monoethyl ether acetate, γ-butyrolactone, polar solvents such as sulfolane, dimethylnaphthalene, dodecylbenzene,
Non-polar solvents such as liquid paraffin, isophorone, kerosene, dibutyl adipate, diethyl phthalate, diethylene glycol monobutyl ether acetate, propylene carbonate, coconut oil, rapeseed oil, cottonseed oil, castor oil and soybean oil can be used as appropriate. Usually, water, alcohols, and a mixed solvent of water and alcohols can be used to dissolve the rare earth salt.

It is more effective to make the composition of the present invention acidic, and for this purpose, it is effective to add an organic acid. As the organic acid, any acid such as citric acid, lactic acid, malic acid, tartaric acid, malonic acid, succinic acid, ascorbic acid, isoascorbic acid, acetic acid, propionic acid, gluconic acid, maleic acid, and fumaric acid can be used. Although it does not matter, citric acid, lactic acid, malic acid, tartaric acid, ascorbic acid and isoascorbic acid are preferred. The content of these organic acids is usually 0.01 to 20% by weight, more preferably 0.1 to 5% by weight. Further, it is effective to add a polymer having a carboxyl group to the preparation of the present invention so that the inactivating effect of the allergen can be stably obtained. Examples of the polymer having a carboxyl group include polyacrylic acid (salt) and polycarboxylic acid-based surfactant. The polyacrylic acid (salt) may be a vinyl monomer containing sulfonic acid or the like, or a polymer copolymerized with maleic acid or the like. Examples of the polycarboxylic acid surfactant include Poise 520 (manufactured by Kao Corporation), Poise 530 (manufactured by Kao Corporation), and Demol EP (manufactured by Kao Corporation). The molecular weight of the polymer is not particularly limited.
00 or more is preferable. The content of the polymer having a carboxyl group is usually 0.01 to 20% by weight, more preferably 0.1 to 5% by weight.

The anti-allergen composition of the present invention can be used in any convenient form such as an aqueous solution, spray, aerosol, paste, powder, etc., but is not particularly limited.

[0010] In the anti-allergen composition of the present invention, other allergen inactivating agents can be mixed and used. Also,
When used for the purpose of removing allergens from indoor dust mites, the effect can be further maintained by mixing with acaricides. The acaricide to be used is not particularly limited as long as it has a lethal effect or a repellent effect on indoor dust mites, for example, benzyl alcohol,
Benzyl benzoate, phenyl salicylate, cinnamaldehyde, hyssop oil, carrot seed oil and the like can also be used, and natural pyrethrin, phenothrin, pyrethroid compounds such as permethrin, fenitrothion,
Organic phosphorus compounds such as malathion, fenthion, and diazinon, dicofol, chlorbenzylate, hexthiazox, tebufenpyrad, and pyridaben can be used.

In the preparation of the present invention, in addition to the organic acid, solvent and polymer having a carboxyl group used,
If necessary, it is also possible to add a surfactant, a chelating agent, a rust inhibitor, an antibacterial agent, a binder, a thickener, a fragrance, a scale inhibitor, an antifoaming agent, an antistatic agent, a softening agent, and the like. .

The environment of the present invention includes carpets, tatami mats,
Bedding such as floors, floor covers, futons, sofas, stuffed animals, clothes, curtains, etc. Indoor living space and the like can be mentioned. As a method of using the anti-allergen composition of the present invention, generally, carpet contaminated with an allergen,
It is conceivable to inactivate the allergen by directly spraying on tatami, bedding, curtains, stuffed animals, etc., but there is no coloring of the object according to the present invention. It is also effective to directly treat bedding and the like contaminated with allergens, such as gluing during washing. Further, a method of laying a cotton cloth or a non-woven fabric treated with the composition of the present invention on bedding is also effective. Before the treatment of the anti-allergen composition, it is desirable to treat miticides having a high degree of contamination with mites, or to wash the whole with a vacuum cleaner or suction with a vacuum cleaner.

The use of the present invention makes it possible to almost completely inactivate allergens derived from mites in house dust, allergens derived from pet hair and epithelium of dogs and cats, cockroaches, feathers, molds, and plant allergens. And can substantially reduce many types of allergens. Therefore, the present invention is particularly effective when the environmental allergen is mite allergen or house allergen in house dust. There are various types of ticks, such as claw mites and mites, of which Dermatophago
ides farinae, Dermatophagoides
Pteronyssinus is considered an important cause of mite allergy. In these mites, not only the mite itself becomes a mite allergen, but also dead carcasses and feces of a mite become very strong allergens. Mold tends to develop in humid places and becomes an allergen when inhaled into the lungs. Examples of the plant allergen include pollen of various plants, and it is known that pollen such as cedar, cypress, ragweed, giant iris, zelkova, mugwort, and harugaya causes allergy.

[0014]

The present invention will be described in more detail with reference to Formulation Examples and Examples, but the present invention is not limited thereto.

[0015]

[Anti-allergen compositions Examples 1 to 18] The components shown in Tables 1 to 3 were thoroughly stirred to obtain a uniform solution. In addition, all the compounding ratios shown in the table are% by weight.

[0016]

Table 1 Examples of anti-allergen compositions * Made by Kao Corporation

[0017]

Table 2 Examples of anti-allergen compositions

[0018]

[Table 3] Examples of anti-allergen compositions

[0019]

[Table 4] Comparative example of anti-allergen composition

[0020]

Test Example 1 Measurement of the Inactivating Effect of the Composition of the Invention on Mite Allergen Der 2 A standard house dust (mite allergen Der 2) was placed on a circularly cut polyester felt 6.6 cm in diameter.
2 containing about 1000 μg / g) of about 0.03 g
Example 1 to Example 1
The anti-allergen composition No. 8 and Comparative Examples 1 to 6 were sprayed in an amount of about 2 g each. After air-drying at room temperature for 5 hours or more, the felt was put into a vinyl bag with a zipper, and a phosphate buffer solution (p
H7.0, bovine serum albumin 15% by weight) 10m
L was added and kneaded well to extract mite allergen. The extract is centrifuged (12,000 rpm x 60 min),
The mite allergen content of the supernatant was determined. To determine the amount of mite allergen, Mighty Checker (manufactured by Shinto Fine Co., Ltd.)
It was used. Table 5 shows the criteria for determining the amount of mite allergen by the mighty checker. Using the supernatant, the amount of mite allergen was measured by the sandwich method of enzyme immunoassay (ELISA). First, De
rf2 monoclonal antibody 13A4 (1000 ng /
1 μL) with a phosphate buffer (pH 7.4, 0.1% by weight N).
aN3) (500 times dilution with F16 MAXIS)
100 μL was added per well of an ORPNUNC-IMMUNO MODULE plate (manufactured by NUNC) and sensitized at 4 ° C. for 1 day or more. After the sensitization, the solution is discarded, and the blocking reagent ｛1% by weight bovine serum albumin F-
V (manufactured by Nacalai Tesque, Inc.) + phosphate buffer (pH
7.2, containing 0.1% by weight of NaN3) was added in an amount of 100 μL per well and reacted at 37 ° C. for 60 minutes. After the reaction, phosphate buffer (pH 7.2, Tween 20)
(Containing 0.1% by weight). To each well, 90 μL of the supernatant sample and 10 μL of a 10-fold concentration of a phosphate buffer were added and reacted at 37 ° C. for 60 minutes. Next, the peroxidase-labeled Derf2 monoclonal antibody was dissolved in distilled water, and the resulting solution was diluted 10-fold with a phosphate buffer (pH 7.2, containing 1% by weight of bovine serum albumin and 0.1% by weight of Tween 20). , 1
100 μL was added per well. After reaction at 37 ° C. for 60 minutes, first, a phosphate buffer (pH 6.2) 15
Orthophenylenediamine dihydrochloride (30 mg Tablet, SIGMA CHEMIC
AL CO. ) And 30% hydrogen peroxide solution (15 μL) were added in an amount of 100 μL per well.
The reaction was performed at 5 ° C. for 5 minutes. Immediately thereafter, 2 mol / L
The reaction was stopped by adding 50 μL of an aqueous sulfuric acid solution, and the spectrophotometer for a microplate (Bio-Rad Labo) was added.
ratories Inc. (OD490)
nm). The same test was conducted except that the examples and comparative examples were not sprayed. The results are shown in Table 6.

[0021]

[Table 5] Mighty checker criteria for mite allergen amount

[0022]

[Table 6] Measurement results of the inactivation effect of the example on mite allergen Der2

[Test Example 2] Measurement of inactivation effect of Example 4 on cedar pollen Cry j2 The inactivation ratio is shown by a reduction ratio with respect to the blank.

Cedar polle extract of cedar pollen
n Extract-Cj 5 μg / 50 μL ｛carbonate-
Example 4 was added to a bicarbonate buffer (pH 9.5)
The reaction was carried out at 16, 20, 24 μL each,
ro / Titertek E.R. I. A. Microt
Iteration plate (ICN BIOCHEMI
CAL INC. (4 ° C., overnight). After the sensitization, the plate was washed with a phosphate buffer (pH 7.2, containing 0.1% by weight of Tween 20). Next, cedar pollen antibody Anti-Cry j-2 (Lot. 7470)
32) was diluted 200-fold with a phosphate buffer (pH 7.2, containing 1% by weight of bovine serum albumin and 0.1% by weight of Tween 20), added at 50 μL / well, and reacted at 37 ° C. for 60 minutes. I let it. After the completion of the reaction, a phosphate buffer (pH 7.2, containing 200.1% by weight of Tween)
The plate was washed. Peroxidase-labeled anti-rabbit
IgG (γ chain) mouse monoclonal antibody HRP-An
ti-Rabbit IgG (SIGMA CHEMI
CALS CO. , Lot. 097H4852) was diluted 5,000-fold with a phosphate buffer (pH 7.2, containing 1% by weight of bovine serum albumin and 0.1% by weight of Tween 20), and 50 μL / well was added thereto.
The reaction was performed in 60 minutes. After completion of the reaction, first, a phosphate buffer (pH 7.2, containing Tween 20 0.1% by weight) was used.
Then, after washing the plate with distilled water, 0.1 mol /
Ortho-phenylenediamine dihydrochloride (30 mg Ta) was added to 15 mL of L phosphate buffer (pH 6.2).
blet, SIGMA CHEMICAL CO. ) And 15 μL of 30% hydrogen peroxide solution were added in an amount of 100 μL per well, and reacted at 37 ° C. for 15 minutes. Immediately thereafter, 50 μL of a 2 mol / L sulfuric acid aqueous solution is used.
The reaction was stopped by adding each solution, and the absorbance (OD 490 nm) was measured using a microplate spectrophotometer. The results are shown in Table 7.

[0024]

[Table 7] Measurement results of the inactivation effect of Example 4 on cedar pollen Cry j2

[0025]

[Test Example 3] Measurement of inactivation effect on mite allergen Der 2 when the composition of the present invention was treated into a carpet Five carpets each having a side of about 50 cm were prepared, and each carpet was vacuumed for 1 minute or more. , Standard house dust (mite allergen Der 2 for about 10
(Containing 00 μg / g) in an amount of about 0.05 g each, and then, using a trigger spray therefrom, Examples 7 and 13, Comparative Example 5
About 40 g of the anti-allergen composition prepared in and 6 was sprayed. After air drying at room temperature for 5 hours or more, the carpet was suctioned for 1 minute with a vacuum cleaner equipped with a dust sampler (manufactured by Shinto Fine Co., Ltd.) and mighty felt (manufactured by Shinto Fine Co., Ltd.). The felt from which the garbage was collected was put into a vinyl bag with a zipper, and 10 mL of a phosphate buffer solution (pH 7.0, containing 10% skim milk and 15% by weight of bovine serum albumin) was added, and the mixture was thoroughly rubbed to extract mite allergen. The extract was centrifuged (12,000 rpm × 60 min), and the amount of mite allergen in the supernatant was measured. Mighty checker and sandwich ELISA methods were used for the measurement. A blank that was similarly tested except that the Examples and Comparative Examples were not sprayed was used as a blank. The results are shown in Table 8.

[0026]

[Table 8] Measurement results of the inactivating effect of the example on mite allergen Der2 Inactivation rate is shown as a reduction ratio to blank

[0027]

[Test Example 4] Discoloration test on cotton fabric Three cotton fabrics each having a side of about 20 cm were treated with Example 7 or a 3% aqueous solution of tannic acid using a trigger spray for about 4 g, and air-dried at room temperature. One was washed with a laundry detergent attack (manufactured by Kao Corporation), and the other was exposed to sunlight for 5 hours. Using a color difference meter CT-210 (manufactured by Minolta Co., Ltd.), the color of the cotton fabric was measured. The closer the L * value (brightness) is to 100, the whiter the color is.
A value (yellow-blueness) that is larger than 0 indicates yellow, and a value smaller than 0 indicates blue. Table 9 shows the results.

[0028]

[Table 9] Discoloration test results

[0029]

According to the present invention, it is possible to inactivate an allergen by treating the anti-allergen composition containing the rare earth salt of the present invention.

Claims (3)

[Claims] 1. An anti-allergen composition comprising a rare earth salt. 2. The anti-allergen composition according to claim 1, comprising 0.01 to 50.0% by weight of a rare earth salt, an organic acid, a polymer having a carboxyl group, alcohols and water. 3. A method for inactivating an allergen, which comprises spraying the anti-allergen composition according to claim 1 or 2 in an environment to inactivate the allergen.