JP5361123B2 - Allergen reducing composition and allergen reducing method - Google Patents
Allergen reducing composition and allergen reducing method Download PDFInfo
- Publication number
- JP5361123B2 JP5361123B2 JP2006268797A JP2006268797A JP5361123B2 JP 5361123 B2 JP5361123 B2 JP 5361123B2 JP 2006268797 A JP2006268797 A JP 2006268797A JP 2006268797 A JP2006268797 A JP 2006268797A JP 5361123 B2 JP5361123 B2 JP 5361123B2
- Authority
- JP
- Japan
- Prior art keywords
- allergen
- mite
- allergens
- composition
- reducing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000000203 mixture Substances 0.000 title claims description 25
- 238000000034 method Methods 0.000 title claims description 12
- 239000013566 allergen Substances 0.000 title description 62
- 230000001603 reducing effect Effects 0.000 title description 7
- 239000000284 extract Substances 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 229930014669 anthocyanidin Natural products 0.000 claims description 4
- 235000008758 anthocyanidins Nutrition 0.000 claims description 4
- 239000003960 organic solvent Substances 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 150000001452 anthocyanidin derivatives Chemical class 0.000 claims description 2
- 229960004784 allergens Drugs 0.000 description 19
- 239000000428 dust Substances 0.000 description 13
- 241000238876 Acari Species 0.000 description 10
- 241000196324 Embryophyta Species 0.000 description 10
- 239000000463 material Substances 0.000 description 9
- 239000008363 phosphate buffer Substances 0.000 description 8
- -1 diiodomethyl paratolyl sulfone Chemical class 0.000 description 7
- 239000000843 powder Substances 0.000 description 7
- 241000219095 Vitis Species 0.000 description 6
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 4
- 241000220225 Malus Species 0.000 description 4
- 235000001630 Pyrus pyrifolia var culta Nutrition 0.000 description 4
- 244000079529 Pyrus serotina Species 0.000 description 4
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 4
- 235000014787 Vitis vinifera Nutrition 0.000 description 4
- 230000000895 acaricidal effect Effects 0.000 description 4
- 239000000642 acaricide Substances 0.000 description 4
- 229940098773 bovine serum albumin Drugs 0.000 description 4
- 239000003638 chemical reducing agent Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000011347 resin Substances 0.000 description 4
- 229920005989 resin Polymers 0.000 description 4
- WURBVZBTWMNKQT-UHFFFAOYSA-N 1-(4-chlorophenoxy)-3,3-dimethyl-1-(1,2,4-triazol-1-yl)butan-2-one Chemical compound C1=NC=NN1C(C(=O)C(C)(C)C)OC1=CC=C(Cl)C=C1 WURBVZBTWMNKQT-UHFFFAOYSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
- 235000011430 Malus pumila Nutrition 0.000 description 3
- 235000015103 Malus silvestris Nutrition 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 240000000851 Vaccinium corymbosum Species 0.000 description 3
- 235000009754 Vitis X bourquina Nutrition 0.000 description 3
- 235000012333 Vitis X labruscana Nutrition 0.000 description 3
- 208000026935 allergic disease Diseases 0.000 description 3
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 230000000855 fungicidal effect Effects 0.000 description 3
- 239000000417 fungicide Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- YEJRWHAVMIAJKC-UHFFFAOYSA-N 4-Butyrolactone Chemical compound O=C1CCCO1 YEJRWHAVMIAJKC-UHFFFAOYSA-N 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- 241000238713 Dermatophagoides farinae Species 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 206010020751 Hypersensitivity Diseases 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- 235000011400 Pyrus pyrifolia Nutrition 0.000 description 2
- 235000004789 Rosa xanthina Nutrition 0.000 description 2
- 241000220222 Rosaceae Species 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 235000003095 Vaccinium corymbosum Nutrition 0.000 description 2
- 235000017537 Vaccinium myrtillus Nutrition 0.000 description 2
- 230000007815 allergy Effects 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- SESFRYSPDFLNCH-UHFFFAOYSA-N benzyl benzoate Chemical compound C=1C=CC=CC=1C(=O)OCC1=CC=CC=C1 SESFRYSPDFLNCH-UHFFFAOYSA-N 0.000 description 2
- FUWUEFKEXZQKKA-UHFFFAOYSA-N beta-thujaplicin Chemical compound CC(C)C=1C=CC=C(O)C(=O)C=1 FUWUEFKEXZQKKA-UHFFFAOYSA-N 0.000 description 2
- LLEMOWNGBBNAJR-UHFFFAOYSA-N biphenyl-2-ol Chemical compound OC1=CC=CC=C1C1=CC=CC=C1 LLEMOWNGBBNAJR-UHFFFAOYSA-N 0.000 description 2
- 235000021014 blueberries Nutrition 0.000 description 2
- 238000013329 compounding Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- NWKFECICNXDNOQ-UHFFFAOYSA-N flavylium Chemical compound C1=CC=CC=C1C1=CC=C(C=CC=C2)C2=[O+]1 NWKFECICNXDNOQ-UHFFFAOYSA-N 0.000 description 2
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 229940087559 grape seed Drugs 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 229930014626 natural product Natural products 0.000 description 2
- 239000004745 nonwoven fabric Substances 0.000 description 2
- 239000006072 paste Substances 0.000 description 2
- ZQBAKBUEJOMQEX-UHFFFAOYSA-N phenyl salicylate Chemical compound OC1=CC=CC=C1C(=O)OC1=CC=CC=C1 ZQBAKBUEJOMQEX-UHFFFAOYSA-N 0.000 description 2
- WVDDGKGOMKODPV-ZQBYOMGUSA-N phenyl(114C)methanol Chemical compound O[14CH2]C1=CC=CC=C1 WVDDGKGOMKODPV-ZQBYOMGUSA-N 0.000 description 2
- 229920000728 polyester Polymers 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- MGSRCZKZVOBKFT-UHFFFAOYSA-N thymol Chemical compound CC(C)C1=CC=C(C)C=C1O MGSRCZKZVOBKFT-UHFFFAOYSA-N 0.000 description 2
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 2
- 229920002554 vinyl polymer Polymers 0.000 description 2
- SBNFWQZLDJGRLK-RTWAWAEBSA-N (1R)-trans-phenothrin Chemical compound CC1(C)[C@H](C=C(C)C)[C@H]1C(=O)OCC1=CC=CC(OC=2C=CC=CC=2)=C1 SBNFWQZLDJGRLK-RTWAWAEBSA-N 0.000 description 1
- KJPRLNWUNMBNBZ-QPJJXVBHSA-N (E)-cinnamaldehyde Chemical compound O=C\C=C\C1=CC=CC=C1 KJPRLNWUNMBNBZ-QPJJXVBHSA-N 0.000 description 1
- GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 1
- TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 description 1
- FPZWZCWUIYYYBU-UHFFFAOYSA-N 2-(2-ethoxyethoxy)ethyl acetate Chemical compound CCOCCOCCOC(C)=O FPZWZCWUIYYYBU-UHFFFAOYSA-N 0.000 description 1
- SBASXUCJHJRPEV-UHFFFAOYSA-N 2-(2-methoxyethoxy)ethanol Chemical compound COCCOCCO SBASXUCJHJRPEV-UHFFFAOYSA-N 0.000 description 1
- XNWFRZJHXBZDAG-UHFFFAOYSA-N 2-METHOXYETHANOL Chemical compound COCCO XNWFRZJHXBZDAG-UHFFFAOYSA-N 0.000 description 1
- DHVLDKHFGIVEIP-UHFFFAOYSA-N 2-bromo-2-(bromomethyl)pentanedinitrile Chemical compound BrCC(Br)(C#N)CCC#N DHVLDKHFGIVEIP-UHFFFAOYSA-N 0.000 description 1
- ZNQVEEAIQZEUHB-UHFFFAOYSA-N 2-ethoxyethanol Chemical compound CCOCCO ZNQVEEAIQZEUHB-UHFFFAOYSA-N 0.000 description 1
- CMNSJKWAGPRSRK-UHFFFAOYSA-N 2-octyl-3h-1,2-thiazole 1-oxide Chemical compound CCCCCCCCN1CC=CS1=O CMNSJKWAGPRSRK-UHFFFAOYSA-N 0.000 description 1
- 229940099451 3-iodo-2-propynylbutylcarbamate Drugs 0.000 description 1
- WYVVKGNFXHOCQV-UHFFFAOYSA-N 3-iodoprop-2-yn-1-yl butylcarbamate Chemical compound CCCCNC(=O)OCC#CI WYVVKGNFXHOCQV-UHFFFAOYSA-N 0.000 description 1
- IJALWSVNUBBQRA-UHFFFAOYSA-N 4-Isopropyl-3-methylphenol Chemical compound CC(C)C1=CC=C(O)C=C1C IJALWSVNUBBQRA-UHFFFAOYSA-N 0.000 description 1
- OSDLLIBGSJNGJE-UHFFFAOYSA-N 4-chloro-3,5-dimethylphenol Chemical compound CC1=CC(O)=CC(C)=C1Cl OSDLLIBGSJNGJE-UHFFFAOYSA-N 0.000 description 1
- 244000036975 Ambrosia artemisiifolia Species 0.000 description 1
- 235000003129 Ambrosia artemisiifolia var elatior Nutrition 0.000 description 1
- 235000003261 Artemisia vulgaris Nutrition 0.000 description 1
- 240000006891 Artemisia vulgaris Species 0.000 description 1
- 241000237519 Bivalvia Species 0.000 description 1
- 241001674044 Blattodea Species 0.000 description 1
- LVDKZNITIUWNER-UHFFFAOYSA-N Bronopol Chemical compound OCC(Br)(CO)[N+]([O-])=O LVDKZNITIUWNER-UHFFFAOYSA-N 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 235000012766 Cannabis sativa ssp. sativa var. sativa Nutrition 0.000 description 1
- 235000012765 Cannabis sativa ssp. sativa var. spontanea Nutrition 0.000 description 1
- 241000218645 Cedrus Species 0.000 description 1
- RAPBNVDSDCTNRC-UHFFFAOYSA-N Chlorobenzilate Chemical compound C=1C=C(Cl)C=CC=1C(O)(C(=O)OCC)C1=CC=C(Cl)C=C1 RAPBNVDSDCTNRC-UHFFFAOYSA-N 0.000 description 1
- 244000301850 Cupressus sempervirens Species 0.000 description 1
- 244000000626 Daucus carota Species 0.000 description 1
- 235000002767 Daucus carota Nutrition 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 206010012438 Dermatitis atopic Diseases 0.000 description 1
- 101000968532 Dictyocaulus viviparus DVA-1 polyprotein Proteins 0.000 description 1
- RUPBZQFQVRMKDG-UHFFFAOYSA-M Didecyldimethylammonium chloride Chemical compound [Cl-].CCCCCCCCCC[N+](C)(C)CCCCCCCCCC RUPBZQFQVRMKDG-UHFFFAOYSA-M 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000001263 FEMA 3042 Substances 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- PNVJTZOFSHSLTO-UHFFFAOYSA-N Fenthion Chemical compound COP(=S)(OC)OC1=CC=C(SC)C(C)=C1 PNVJTZOFSHSLTO-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 1
- 239000005949 Malathion Substances 0.000 description 1
- 244000070406 Malus silvestris Species 0.000 description 1
- FXHOOIRPVKKKFG-UHFFFAOYSA-N N,N-Dimethylacetamide Chemical compound CN(C)C(C)=O FXHOOIRPVKKKFG-UHFFFAOYSA-N 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 1
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 108010039918 Polylysine Proteins 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- VQXSOUPNOZTNAI-UHFFFAOYSA-N Pyrethrin I Natural products CC(=CC1CC1C(=O)OC2CC(=O)C(=C2C)CC=C/C=C)C VQXSOUPNOZTNAI-UHFFFAOYSA-N 0.000 description 1
- 239000005663 Pyridaben Substances 0.000 description 1
- 244000088401 Pyrus pyrifolia Species 0.000 description 1
- 241001301992 Rana clamitans Species 0.000 description 1
- 241000208422 Rhododendron Species 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 239000005658 Tebufenpyrad Substances 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 235000009392 Vitis Nutrition 0.000 description 1
- 240000002503 Vitis amurensis Species 0.000 description 1
- 241001512058 Vitis labrusca x Vitis vinifera Species 0.000 description 1
- 244000068697 Vitis rotundifolia Species 0.000 description 1
- 235000006359 Vitis rotundifolia var rotundifolia Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 241000190021 Zelkova Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 235000011054 acetic acid Nutrition 0.000 description 1
- 238000004026 adhesive bonding Methods 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 229940074608 allergen extract Drugs 0.000 description 1
- 230000002009 allergenic effect Effects 0.000 description 1
- 230000000172 allergic effect Effects 0.000 description 1
- TUFYVOCKVJOUIR-UHFFFAOYSA-N alpha-Thujaplicin Natural products CC(C)C=1C=CC=CC(=O)C=1O TUFYVOCKVJOUIR-UHFFFAOYSA-N 0.000 description 1
- 235000003484 annual ragweed Nutrition 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 235000021016 apples Nutrition 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 201000008937 atopic dermatitis Diseases 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- 229960000686 benzalkonium chloride Drugs 0.000 description 1
- DMSMPAJRVJJAGA-UHFFFAOYSA-N benzo[d]isothiazol-3-one Chemical compound C1=CC=C2C(=O)NSC2=C1 DMSMPAJRVJJAGA-UHFFFAOYSA-N 0.000 description 1
- 229960002903 benzyl benzoate Drugs 0.000 description 1
- CADWTSSKOVRVJC-UHFFFAOYSA-N benzyl(dimethyl)azanium;chloride Chemical compound [Cl-].C[NH+](C)CC1=CC=CC=C1 CADWTSSKOVRVJC-UHFFFAOYSA-N 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 235000006263 bur ragweed Nutrition 0.000 description 1
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 235000009120 camo Nutrition 0.000 description 1
- 229960001927 cetylpyridinium chloride Drugs 0.000 description 1
- YMKDRGPMQRFJGP-UHFFFAOYSA-M cetylpyridinium chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCC[N+]1=CC=CC=C1 YMKDRGPMQRFJGP-UHFFFAOYSA-M 0.000 description 1
- 235000005607 chanvre indien Nutrition 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229960003333 chlorhexidine gluconate Drugs 0.000 description 1
- YZIYKJHYYHPJIB-UUPCJSQJSA-N chlorhexidine gluconate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O.C1=CC(Cl)=CC=C1NC(=N)NC(=N)NCCCCCCNC(=N)NC(=N)NC1=CC=C(Cl)C=C1 YZIYKJHYYHPJIB-UUPCJSQJSA-N 0.000 description 1
- DHNRXBZYEKSXIM-UHFFFAOYSA-N chloromethylisothiazolinone Chemical compound CN1SC(Cl)=CC1=O DHNRXBZYEKSXIM-UHFFFAOYSA-N 0.000 description 1
- 229940117916 cinnamic aldehyde Drugs 0.000 description 1
- KJPRLNWUNMBNBZ-UHFFFAOYSA-N cinnamic aldehyde Natural products O=CC=CC1=CC=CC=C1 KJPRLNWUNMBNBZ-UHFFFAOYSA-N 0.000 description 1
- 235000020639 clam Nutrition 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 235000003488 common ragweed Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- NMCCNOZOBBWFMN-UHFFFAOYSA-N davicil Chemical compound CS(=O)(=O)C1=C(Cl)C(Cl)=NC(Cl)=C1Cl NMCCNOZOBBWFMN-UHFFFAOYSA-N 0.000 description 1
- FHIVAFMUCKRCQO-UHFFFAOYSA-N diazinon Chemical compound CCOP(=S)(OCC)OC1=CC(C)=NC(C(C)C)=N1 FHIVAFMUCKRCQO-UHFFFAOYSA-N 0.000 description 1
- 229960004670 didecyldimethylammonium chloride Drugs 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- JXSJBGJIGXNWCI-UHFFFAOYSA-N diethyl 2-[(dimethoxyphosphorothioyl)thio]succinate Chemical compound CCOC(=O)CC(SP(=S)(OC)OC)C(=O)OCC JXSJBGJIGXNWCI-UHFFFAOYSA-N 0.000 description 1
- 229940028356 diethylene glycol monobutyl ether Drugs 0.000 description 1
- XXJWXESWEXIICW-UHFFFAOYSA-N diethylene glycol monoethyl ether Chemical compound CCOCCOCCO XXJWXESWEXIICW-UHFFFAOYSA-N 0.000 description 1
- 229940075557 diethylene glycol monoethyl ether Drugs 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- SZXQTJUDPRGNJN-UHFFFAOYSA-N dipropylene glycol Chemical compound OCCCOCCCO SZXQTJUDPRGNJN-UHFFFAOYSA-N 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 210000003746 feather Anatomy 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- ZNOLGFHPUIJIMJ-UHFFFAOYSA-N fenitrothion Chemical compound COP(=S)(OC)OC1=CC=C([N+]([O-])=O)C(C)=C1 ZNOLGFHPUIJIMJ-UHFFFAOYSA-N 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 229940074391 gallic acid Drugs 0.000 description 1
- 235000004515 gallic acid Nutrition 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 229940087603 grape seed extract Drugs 0.000 description 1
- 235000002532 grape seed extract Nutrition 0.000 description 1
- 235000013761 grape skin extract Nutrition 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 239000011487 hemp Substances 0.000 description 1
- 239000012456 homogeneous solution Substances 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 239000001735 hyssopus officinalis l. herb oil Substances 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 229960000453 malathion Drugs 0.000 description 1
- KBHDSWIXRODKSZ-UHFFFAOYSA-N methyl 5-chloro-2-(trifluoromethylsulfonylamino)benzoate Chemical compound COC(=O)C1=CC(Cl)=CC=C1NS(=O)(=O)C(F)(F)F KBHDSWIXRODKSZ-UHFFFAOYSA-N 0.000 description 1
- VTYLJMXGYASCLI-UHFFFAOYSA-N methyl N-(1H-benzimidazol-2-yl)carbamate 2,4,5,6-tetrachlorobenzene-1,3-dicarbonitrile Chemical compound C1=CC=C2NC(NC(=O)OC)=NC2=C1.ClC1=C(Cl)C(C#N)=C(Cl)C(C#N)=C1Cl VTYLJMXGYASCLI-UHFFFAOYSA-N 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- JWZXKXIUSSIAMR-UHFFFAOYSA-N methylene bis(thiocyanate) Chemical compound N#CSCSC#N JWZXKXIUSSIAMR-UHFFFAOYSA-N 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 150000002903 organophosphorus compounds Chemical class 0.000 description 1
- 239000004306 orthophenyl phenol Substances 0.000 description 1
- 235000010292 orthophenyl phenol Nutrition 0.000 description 1
- JCGNDDUYTRNOFT-UHFFFAOYSA-N oxolane-2,4-dione Chemical compound O=C1COC(=O)C1 JCGNDDUYTRNOFT-UHFFFAOYSA-N 0.000 description 1
- RLLPVAHGXHCWKJ-UHFFFAOYSA-N permethrin Chemical compound CC1(C)C(C=C(Cl)Cl)C1C(=O)OCC1=CC=CC(OC=2C=CC=CC=2)=C1 RLLPVAHGXHCWKJ-UHFFFAOYSA-N 0.000 description 1
- 229960000490 permethrin Drugs 0.000 description 1
- 102000013415 peroxidase activity proteins Human genes 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 238000001050 pharmacotherapy Methods 0.000 description 1
- 229960003536 phenothrin Drugs 0.000 description 1
- 229960000969 phenyl salicylate Drugs 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 239000004584 polyacrylic acid Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920000656 polylysine Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- HYJYGLGUBUDSLJ-UHFFFAOYSA-N pyrethrin Natural products CCC(=O)OC1CC(=C)C2CC3OC3(C)C2C2OC(=O)C(=C)C12 HYJYGLGUBUDSLJ-UHFFFAOYSA-N 0.000 description 1
- VJFUPGQZSXIULQ-XIGJTORUSA-N pyrethrin II Chemical compound CC1(C)[C@H](/C=C(\C)C(=O)OC)[C@H]1C(=O)O[C@@H]1C(C)=C(C\C=C/C=C)C(=O)C1 VJFUPGQZSXIULQ-XIGJTORUSA-N 0.000 description 1
- 239000002728 pyrethroid Substances 0.000 description 1
- DWFZBUWUXWZWKD-UHFFFAOYSA-N pyridaben Chemical compound C1=CC(C(C)(C)C)=CC=C1CSC1=C(Cl)C(=O)N(C(C)(C)C)N=C1 DWFZBUWUXWZWKD-UHFFFAOYSA-N 0.000 description 1
- YBBJKCMMCRQZMA-UHFFFAOYSA-N pyrithione Chemical compound ON1C=CC=CC1=S YBBJKCMMCRQZMA-UHFFFAOYSA-N 0.000 description 1
- 235000009736 ragweed Nutrition 0.000 description 1
- 239000005871 repellent Substances 0.000 description 1
- 230000002940 repellent Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- HXJUTPCZVOIRIF-UHFFFAOYSA-N sulfolane Chemical compound O=S1(=O)CCCC1 HXJUTPCZVOIRIF-UHFFFAOYSA-N 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 235000015523 tannic acid Nutrition 0.000 description 1
- LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 description 1
- 229940033123 tannic acid Drugs 0.000 description 1
- 229920002258 tannic acid Polymers 0.000 description 1
- ZZYSLNWGKKDOML-UHFFFAOYSA-N tebufenpyrad Chemical compound CCC1=NN(C)C(C(=O)NCC=2C=CC(=CC=2)C(C)(C)C)=C1Cl ZZYSLNWGKKDOML-UHFFFAOYSA-N 0.000 description 1
- WJCNZQLZVWNLKY-UHFFFAOYSA-N thiabendazole Chemical compound S1C=NC(C=2NC3=CC=CC=C3N=2)=C1 WJCNZQLZVWNLKY-UHFFFAOYSA-N 0.000 description 1
- ZIBGPFATKBEMQZ-UHFFFAOYSA-N triethylene glycol Chemical compound OCCOCCOCCO ZIBGPFATKBEMQZ-UHFFFAOYSA-N 0.000 description 1
- 239000001717 vitis vinifera seed extract Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
- 150000003751 zinc Chemical class 0.000 description 1
- 229930007845 β-thujaplicin Natural products 0.000 description 1
Landscapes
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
- Filtering Materials (AREA)
Description
本発明は、環境中のアレルゲンを低減化するための抗アレルゲン組成物及びこれを用いたアレルゲン低減化方法に関するものである。 The present invention relates to an anti-allergen composition for reducing allergens in the environment and a method for reducing allergens using the same.
喘息やアトピー性皮膚炎などのアレルギー性疾患は、長年にわたり、多くの人が悩まされてきたものである。これらのアレルギー疾患の原因物質(以下アレルゲンと示す)の代表的なものとしては、屋内に棲息するダニやペットの毛、花粉などがよく知られている。現在、アレルギー患者の治療には主に薬物療法が適用されている一方、原因物質であるアレルゲンを患者自身の生活環境から除去することも、患者をアレルゲンの暴露から直接守るという合理的な手段である。このようなアレルゲン除去による症状改善は、日本の他、ヨーロッパやアメリカにおいても報告がなされている。 Allergic diseases such as asthma and atopic dermatitis have been plagued by many people for many years. As typical examples of the causative substances (hereinafter referred to as allergens) of these allergic diseases, mites, pet hair, pollen and the like that are indoors are well known. Currently, pharmacotherapy is mainly applied to the treatment of allergic patients, but removing the causative substance allergen from the patient's own living environment is also a rational means of protecting the patient directly from allergen exposure. is there. Such symptom improvement by allergen removal has been reported not only in Japan but also in Europe and the United States.
アレルゲン除去の方法としては、電気掃除機による吸引、空気清浄機による除去や寝具の高密度カバーの使用などがあげられる。しかしながら、電気掃除機による吸引だけでは除去できるアレルゲン量に限界があり、空気清浄機では空中に舞うアレルゲン除去しかできない。また、寝具の高密度カバーでは、内側からのアレルゲン除去にはなるが、外側からのアレルゲン除去にはならないなど、これらの方法は必ずしも満足できるものではなかった。 Examples of the allergen removal method include suction with an electric vacuum cleaner, removal with an air cleaner, and use of a high-density bedding cover. However, there is a limit to the amount of allergen that can be removed only by suction with a vacuum cleaner, and an air cleaner can only remove allergens that fly in the air. In addition, although the allergen removal from the inside is possible with the high-density cover of the bedding, these methods are not always satisfactory, such as not removing the allergen from the outside.
アレルゲンを低減化できる天然物としては、特許文献1にはタンニン酸、特許文献2には茶抽出物、没食子酸等を用いた方法が提案されている。しかしながら、これらの天然物では安定したアレルゲン低減化効果を得ることが困難である。 As natural products that can reduce allergens, Patent Document 1 proposes a method using tannic acid, and Patent Document 2 uses a tea extract, gallic acid, or the like. However, it is difficult to obtain a stable allergen reducing effect with these natural products.
また、ハウスダスト中のダニ駆除には一般的に殺ダニ剤が用いられるが、ハウスダスト中のコナヒョウヒダニDermatophagoides farinaeやヤケヒョウヒダニDermatophagpides pteroynyssinus等は、死んだ後もアレルゲン性を有し、虫体が分解するに従い、徐々に微粒子のアレルゲンを放出するため、ダニを殺しただけではアレルゲンを低減化したことにはならない。 In addition, acaricides are generally used to control ticks in house dust, but Dermatophagoides farinae, Dermatophagides dermatophagpides pteronyssinus, etc. in house dust have allergenic properties even after they die, and the insect body decomposes. Accordingly, the allergen in the form of fine particles is gradually released. Therefore, simply killing the mite does not reduce the allergen.
アレルゲン低減化組成物及びそれを使用したアレルゲン低減化方法を提供する。また、乾燥条件においても環境中のハウスダスト、ダニ、花粉等に由来するアレルゲンを効率的に低減化するアレルゲン低減化組成物及びそれを加工した抗アレルゲンフィルター材またはシートを提供することが課題である。 An allergen-reducing composition and an allergen-reducing method using the same are provided. Another problem is to provide an allergen-reducing composition that efficiently reduces allergens derived from house dust, mites, pollen, etc. in the environment even under dry conditions, and an anti-allergen filter material or sheet processed therefrom. is there.
本発明者は上記課題を解決するために鋭意検討した結果、ブドウ科ブドウ属から選ばれる植物の種子または果皮の水および/または有機溶媒抽出物が、種々のアレルゲンを低減化することを見出した。さらに、これらの抽出物成分についても種々検討したところ、抽出物中に含まれるアントシアニジンが特に高いアレルゲン低減化効果を有することを見出した。またこれらのアレルゲン低減化剤を室内環境中、例えば、フィルター、マスク、シート、たたみ、じゅうたん、ふとん等に処理することによりアレルゲンを除去できることを見出し本発明に至った。すなわち本発明は、ブドウ科ブドウ属から選ばれる少なくとも1種の植物の種子または果皮の抽出物からなることを特徴とするアレルゲン低減化剤に関するものである。 As a result of intensive studies to solve the above problems, the present inventor has found that water and / or organic solvent extracts of plant seeds or skins selected from the genus Grapeaceae reduce various allergens. . Furthermore, various investigations were conducted on these extract components, and it was found that anthocyanidins contained in the extract have a particularly high allergen reducing effect. Further, the present inventors have found that allergens can be removed by treating these allergen reducing agents in an indoor environment, for example, filters, masks, sheets, tatami, carpets, futons, etc. That is, the present invention relates to an allergen reducing agent comprising an extract of seeds or peels of at least one plant selected from the genus Grapeaceae.
ブドウ科ブドウ属から選ばれる少なくとも1種の植物の種子または果皮の抽出物からなるアレルゲン低減化組成物を処理することにより、アレルゲンを低減化することが可能になった。 It has become possible to reduce allergens by treating an allergen-reducing composition comprising an extract of seeds or peels of at least one plant selected from the genus Grapeaceae.
本発明に用いるブドウ科 Vitaceaeブドウ属 Vitis植物としてはヤマブドウ Vitis coignetiae、ヨーロッパ・ブドウ V. vinifera、アメリカ・ブドウ V. labrusea、マスカダイン V. rotundifolia、マンシュウヤマブドウ V. amurensis、エビヅル V. ficifolia等があげられる。これらの植物の種子または果皮は植物から直接採取しても良いし、乾燥粉末を用いても良い。これらの種子、果皮またはそれらの乾燥粉末を水および/または有機溶媒で抽出した抽出物を用いることもできる。抽出に適する溶媒は例えば水、メチルアルコール、エチルアルコール、イソプロピルアルコール、ベンジルアルコール、酢酸、アセトン、ジメチルホルムアミド、ジメチルアセトアミド、ジメチルスルホキシド、エチレングリコール、プロピレングリコール、ジエチレングリコール、トリエチレングリコール、ジプロピレングリコール、ヘキシレングリコール、ポリエチレングリコール、グリセリン、エチレングリコールモノメチルエーテル、エチレングリコールモノエチルエーテル、ジエチレングリコールモノメチルエーテル、ジエチレングリコールモノエチルエーテル、ジエチレングリコールモノブチルエーテル、ジエチレングリコールモノエチルエーテルアセテート、γ−ブチロラクトン、スルホランなどを適宜使用でき、これらに限定されない。 Vitaceae Vitaceae grape genus Vitis plants used in the present invention include Yama grape, Vitis coigneiae, and European grapes. vinifera, American grapes labrusea, muscadine rotundifolia, Manshuyama grape V. amurensis, shrimp ficifolia and the like. The seeds or pericarps of these plants may be collected directly from the plant, or dry powder may be used. An extract obtained by extracting these seeds, pericarp or dry powder thereof with water and / or an organic solvent can also be used. Suitable solvents for extraction are, for example, water, methyl alcohol, ethyl alcohol, isopropyl alcohol, benzyl alcohol, acetic acid, acetone, dimethylformamide, dimethylacetamide, dimethyl sulfoxide, ethylene glycol, propylene glycol, diethylene glycol, triethylene glycol, dipropylene glycol, Xylene glycol, polyethylene glycol, glycerin, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, diethylene glycol monobutyl ether, diethylene glycol monoethyl ether acetate, γ-butyrolactone, sulfolane, etc. can be used as appropriate. Limited to Not.
これらの植物の種子、果皮やその抽出物またはアントシアニジンはそのまま用いても良いが、一般には適当な形に製剤して用いる。またその使用形態としては、水性溶液、スプレー、エアゾール、ペースト、粉剤等、都合の良い形にてきるが、特に限定されるものではない。またフィルター材またはシートに加工する場合にも、液状、粉体状及びペースト状等、どのような剤型でも差し支えないが、液状とするのが最も加工が容易で有効である。本発明のアレルゲン低減化組成物をフィルター材またはシート材に加工したとき、乾燥後の粉立ちを防ぐために水溶性の樹脂を添加することが好ましい。このような水溶性樹脂は特に限定されないが、ポリビニルアルコール、ポリアクリル酸(塩)、ポリエチレングリコール、ポリビニルピロリドン等が挙げられる。 These plant seeds, pericarps, extracts thereof, or anthocyanidins may be used as they are, but they are generally used in a suitable form. In addition, the usage form is not particularly limited, although it may be in a convenient form such as an aqueous solution, spray, aerosol, paste, powder or the like. Also, when processing into a filter material or a sheet, any dosage form such as liquid, powder, and paste may be used, but liquid processing is the easiest and most effective. When the allergen-reducing composition of the present invention is processed into a filter material or a sheet material, it is preferable to add a water-soluble resin in order to prevent powdering after drying. Such a water-soluble resin is not particularly limited, and examples thereof include polyvinyl alcohol, polyacrylic acid (salt), polyethylene glycol, and polyvinyl pyrrolidone.
本発明アレルゲン低減化組成物をフィルターまたはシートに加工した場合、カビあるいは菌の増殖が懸念される場合がある。そのために防カビ剤または抗菌剤を本発明の抗アレルゲン組成物に配合することもしくは併用することが可能である。防カビ剤または抗菌剤の種類は防カビまたは抗菌効果を有するものであれば特に限定されないが、例えば、5−クロロ−N−メチルイソチアゾロン、メチレンビスチオシアネート、2−ブロモ−2−ニトロプロパン−1,3−ジオール、グルタルアルデヒド、ヨードプロピニルブチルカーバメート、ピリジンチオール−N−オキシドの亜鉛塩、1,2−ベンゾイソチアゾロン、1,2−ジブロモ−2,4−ジシアノブタン、グルコン酸クロルヘキシジン、2−イソプロピル−5−メチルフェノール、3−メチル−4−イソプロピルフェノール、オルトフェニルフェノール、パラオキシ安息香酸メチル、パラオキシ安息香酸エチル、パラオキシ安息香酸プロピル、パラオキシ安息香酸ブチル、パラクロロメタキシレノール、パラクロロメタクレゾール、ポリリジン、塩化ベンザルコニウム、塩化ジデシルジメチルアンモニウム、塩化セチルピリジニウム、N−n−ブチルベンゾイソチアゾロン、N−オクチルイソチアゾロン、2−(4−チアゾリル)ベンズイミダゾール、2−ベンズイミダゾリルカルバミン酸メチル、テトラクロロイソフタロニトリル、ジヨードメチルパラトリルスルホン、パラクロロフェニル−3−ヨードプロパギルホルマール、2,3,5,6−テトラクロロ−4−(メチルスルホニル)ピリジン、脂肪酸グリセリンエステル、ヒノキチオール等を用いることができる。 When the allergen-reducing composition of the present invention is processed into a filter or sheet, there is a possibility that mold or fungus grows. Therefore, it is possible to mix or use a fungicide or an antibacterial agent in the anti-allergen composition of the present invention. The type of the fungicide or antibacterial agent is not particularly limited as long as it has a fungicide or antibacterial effect. For example, 5-chloro-N-methylisothiazolone, methylenebisthiocyanate, 2-bromo-2-nitropropane-1 , 3-diol, glutaraldehyde, iodopropynyl butyl carbamate, zinc salt of pyridinethiol-N-oxide, 1,2-benzisothiazolone, 1,2-dibromo-2,4-dicyanobutane, chlorhexidine gluconate, 2-isopropyl -5-methylphenol, 3-methyl-4-isopropylphenol, orthophenylphenol, methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, butyl paraoxybenzoate, parachlorometaxylenol, parachlorometacres , Polylysine, benzalkonium chloride, didecyldimethylammonium chloride, cetylpyridinium chloride, Nn-butylbenzoisothiazolone, N-octylisothiazolone, 2- (4-thiazolyl) benzimidazole, methyl 2-benzimidazolylcarbamate Tetrachloroisophthalonitrile, diiodomethyl paratolyl sulfone, parachlorophenyl-3-iodopropargyl formal, 2,3,5,6-tetrachloro-4- (methylsulfonyl) pyridine, fatty acid glycerin ester, hinokitiol, etc. Can be used.
本発明アレルゲン低減化組成物には、他のアレルゲン低減化剤を混合して用いることもできる。また、屋内塵性ダニのアレルゲン除去を目的に使用する場合には、殺ダニ剤と混合することにより、その効果をさらに持続させることも可能である。使用する殺ダニ剤は、屋内塵性ダニに対して致死効果や忌避効果のあるものであれば、特に限定はなく、例えば、ベンジルアルコール、ベンジルベンゾエート、サリチル酸フェニル、シンナムアルデヒド、ヒソップ油、ニンジン種子油等を用いることができ、また天然ピレトリン、フェノトリン、ペルメトリン等のピレスロイド系化合物、フェニトロチオン、マラチオン、フェンチオン、ダイアジノン等の有機リン系化合物、ジコホル、クロルベンジレート、ヘキシチアゾクス、テブフェンピラド、ピリダベン、アミドフルメト等を用いることができる。 The allergen-reducing composition of the present invention can be used by mixing with other allergen-reducing agents. Moreover, when using it for the purpose of allergen removal of an indoor dust mite, the effect can be further continued by mixing with an acaricide. The acaricide used is not particularly limited as long as it has a lethal and repellent effect on indoor dust mites, such as benzyl alcohol, benzyl benzoate, phenyl salicylate, cinnamaldehyde, hyssop oil, carrot seeds. Oils can be used, and pyrethroid compounds such as natural pyrethrin, phenothrin, and permethrin, organophosphorus compounds such as fenitrothion, malathion, fenthion, and diazinon, dicophore, chlorbenzilate, hexithiazox, tebufenpyrad, pyridaben, and amidoflumet. Can be used.
本発明の環境としては、カーペット、畳、床面、床カバー、布団などの寝具類、ソファー、ぬいぐるみ、衣類、カーテンなど直接人と接触するもの、あるいはタンス、押し入れなどの直接人と接触するものを収納している場所の空間、あるいは家屋内の居住空間などが挙げられる。本発明の抗アレルゲン組成物の使用方法として、一般にはアレルゲンで汚染されたカーペット、畳、寝具類、カーテン、ぬいぐるみ等に直接散布してアレルゲンを低減化することが考えられる。また、アレルゲンで汚染された寝具類等には、洗濯時の糊付けのように直接処理する方法も効果的である。さらに、本発明組成物を処理した綿布や不織布などを寝具類の上に敷く方法も効果的である。ダニによる汚染度の高いものは、アレルゲン低減化組成物を処理する前に、殺ダニ剤を処理したり、丸洗いや電気掃除機による吸引などをするのが望ましい。 Environments of the present invention include carpets, tatami mats, floors, floor covers, futon bedding, sofas, stuffed animals, clothing, curtains, etc. that are in direct contact with humans, such as clothes, closets, etc. The space of the place where the house is stored or the living space in the house. As a method of using the anti-allergen composition of the present invention, it is generally considered to reduce the allergen by directly spraying it on carpets, tatami mats, bedding, curtains, stuffed animals and the like contaminated with allergens. In addition, for bedding contaminated with allergens, a method of direct treatment such as gluing at the time of washing is also effective. Furthermore, a method of laying a cotton cloth or nonwoven fabric treated with the composition of the present invention on bedding is also effective. Those having a high degree of contamination with mites are preferably treated with an acaricide, or washed with a vacuum or a vacuum cleaner before treating the allergen-reducing composition.
本発明の使用により、ハウスダスト中のダニ由来のアレルゲン、イヌやネコなどのペットの毛や上皮、ゴキブリ、羽毛、カビ由来のアレルゲン、及び植物アレルゲンをほぼ完全に低減化することができ、多種のアレルゲンを実質的に減少させることができる。よって本発明は、環境中のアレルゲンがハウスダスト中のダニアレルゲンや植物アレルゲンの場合に特に効果的に作用するものである。ダニには、ツメダニ、コナダニ等の種類があり、それらの内コナヒョウヒダニ Dermatophagoides farinaeやヤケヒョウヒダニDermatophagpides pteroynyssinusはダニアレルギーを引き起こす原因として重要視されている。これらのダニは、虫体そのものがダニアレルゲンになるだけでなく、ダニの死骸や糞も非常に強いアレルゲンとなる。カビは湿度の高い場所に発生しやすく、肺に吸い込まれた場合にはアレルゲンとなる。植物アレルゲンとしては各種植物の花粉が挙げられ、花粉には、スギ、ヒノキ、ブタクサ、オオアワガエリ、ケヤキ、ヨモギ、ハルガヤ等のものがアレルギーの原因となることが知られている。 By using the present invention, allergens derived from ticks in house dust, pet hair and epithelium such as dogs and cats, cockroaches, feathers, molds, and plant allergens can be almost completely reduced. Allergens can be substantially reduced. Therefore, the present invention works particularly effectively when the allergen in the environment is a mite allergen or a plant allergen in house dust. There are various types of mites, such as clams and mites. Among them, Dermatophagoides farinae and Dermatophagides pteronyssinus are regarded as important causes of mite allergy. These mites are not only mite allergens, but also mite dead bodies and feces are very strong allergens. Mold is likely to occur in humid places and becomes an allergen when inhaled into the lungs. Examples of plant allergens include pollen of various plants, and it is known that pollen such as cedar, cypress, ragweed, green frog, zelkova, mugwort, and hurghaya causes allergies.
アレルゲンを除去、低減化するフィルター材またはシートの用途に関して特に制限されず、例えば空気清浄機、エアコンディショナー等のフィルター、マスク、集塵袋、掃除用ワイパー等任意の用途に用いることができる。フィルター材またはシート材には、ポリエチレン、ポリプロピレン、ポリエステル、ナイロン、ポリカーボネート等のプラスチック材から成る不織布、成型した網を用いることができ、また紙、綿、麻、絹等の天然繊維、さらにガラス繊維等の無機材料を用いることも可能である。これらの材を目的に応じ適宜選択することができる。 The use of the filter material or sheet for removing and reducing allergens is not particularly limited, and can be used for any application such as a filter such as an air cleaner or an air conditioner, a mask, a dust bag, or a cleaning wiper. The filter material or sheet material can be non-woven fabrics and molded nets made of plastic materials such as polyethylene, polypropylene, polyester, nylon, polycarbonate, etc., and natural fibers such as paper, cotton, hemp, silk, and glass fibers. It is also possible to use inorganic materials such as. These materials can be appropriately selected according to the purpose.
本発明を製剤例、実施例により更に説明するが、本発明がこれらによって限定されることはない。 The present invention will be further described with reference to formulation examples and examples, but the present invention is not limited thereto.
<抗アレルゲン組成物実施例1〜3>表1に示す組成のものを、充分攪拌することにより、均一な溶液を得た。なお、ブドウ果皮抽出液及びブドウ種子抽出液は水100g中にそれぞれブドウの果皮を5g、種子を0.1g入れ、ミキサーで粉砕し、その液を濾紙で濾過したろ液のことである。ブドウ種子乾燥粉末濾液は、ブドウ種子乾燥粉末5gを蒸留水95g中に入れ、その懸濁液をろ過した液である。表に示した配合比率はすべて重量%である。 <Anti-allergen compositions Examples 1 to 3> The compositions shown in Table 1 were sufficiently stirred to obtain a uniform solution. The grape skin extract and grape seed extract are filtrates obtained by putting 5 g of grape skin and 0.1 g of seeds in 100 g of water, pulverizing them with a mixer, and filtering the solution with filter paper. The grape seed dry powder filtrate is a liquid obtained by putting 5 g of grape seed dry powder in 95 g of distilled water and filtering the suspension. All the compounding ratios shown in the table are% by weight.
<抗アレルゲン組成物実施例4>アントシアニジンを90%以上含有する粉末(ビノフェロン、株式会社常磐植物化学研究所製)5gを蒸留水95g中に溶解し攪拌することにより均一な溶液を得た。 <Anti-allergen composition example 4> A homogeneous solution was obtained by dissolving 5 g of powder containing 90% or more of anthocyanidin (Binoferon, manufactured by Joban Plant Chemistry Laboratory Co., Ltd.) in 95 g of distilled water and stirring.
<抗アレルゲン組成物比較例1〜7>表2に示す組成のものを、充分攪拌することにより、均一な溶液を得た。リンゴ Malus pumilaはバラ科リンゴ属、ニホンナシ Pyrus pyrifoliaはバラ科ナシ属、ブルーベリーVaccinium corymbosumはツツジ科スノキ属である。なお、各抽出液は水100g中にそれぞれブドウ、リンゴ、ニホンナシ及びブルーベリーの果実を5g、リンゴ、ニホンナシ及びブルーベリーの果皮を5g、リンゴ、ニホンナシの種子を0.1g入れ、ミキサーで粉砕し、その液を濾紙で濾過したろ液のことである。表に示した配合比率はすべて重量%である。 <Antiallergen Composition Comparative Examples 1 to 7> Thoroughly stirring the compositions shown in Table 2 yielded a uniform solution. Apples Malus pumila is a genus of the family Rosaceae, Japanese pear Pyrus pyrifolia is a genus of the family Rosaceae, and Blueberry Vaccinium corymbosum is a genus of Azalea. In addition, 5 g of grape, apple, Japanese pear and blueberry fruits, 5 g of apple, Japanese pear and blueberry peels, and 0.1 g of apple and Japanese pear seeds were put in 100 g of water, and each extract was pulverized with a mixer. It is the filtrate which filtered the liquid with the filter paper. All the compounding ratios shown in the table are% by weight.
<試験例1>ダニアレルゲン対する本発明の組成物の不活化効果の測定1標準ハウスダスト(ダニアレルゲンDer2を約1000μg/g含有)およそ5gを、リン酸緩衝液(pH7.0)100mLに入れてダニアレルゲンを抽出した。ダニアレルゲン抽出液1mLに実施例1〜4及び比較例1〜7をそれぞれ150μg添加し、ダニアレルゲン量の判定を行った。ダニアレルゲン量の判定には、屋内塵性ダニ簡易検査キットであるマイティチェッカー(シントーファイン株式会社製)を使用した。マイティチェッカーによるダニアレルゲン量の判定基準は、表3の通りである。結果を表4に示した。 <Test Example 1> Measurement of the inactivation effect of the composition of the present invention against mite allergen 1 About 5 g of standard house dust (containing mite allergen Der2 of about 1000 μg / g) was put in 100 mL of phosphate buffer (pH 7.0). Mite allergen was extracted. 150 μg of each of Examples 1 to 4 and Comparative Examples 1 to 7 was added to 1 mL of mite allergen extract, and the amount of mite allergen was determined. Mite checker (manufactured by Shinto Fine Co., Ltd.), which is a simple indoor dust mite test kit, was used to determine the amount of mite allergen. Table 3 shows the criteria for determining the mite allergen amount by the Mighty Checker. The results are shown in Table 4.
<試験例2>ダニアレルゲン Der 2 に対する本発明の組成物の低減化効果の測定2
直径6.6cmの円形に切り取ったポリエステル製のフェルトに、標準ハウスダスト(ダニアレルゲン Der 2 を約1000μg/g含有)をおよそ0.03gずつのせ、そこへトリガースプレーにて、実施例1〜3及び比較例1のアレルゲン低減化組成物及を約2g散布した。5時間以上、室温で自然乾燥させた後、フェルトをチャック付きビニル袋に入れ、リン酸緩衝液(pH7.0、牛血清アルブミン 15重量%含有)10mLを加えてよく揉み、ダニアレルゲンを抽出した。抽出液を遠心分離器にかけ(12,000rpm×60min)、上澄み液を用い、酵素免疫測定法(ELISA法)のサンドイッチ法にてダニアレルゲン量の測定を行った。まず、Derf2 モノクローナル抗体13A4(1000ng/1μL)をリン酸緩衝液(pH7.4、0.1重量%NaN3含有)で500倍に希釈し、F16 MAXISORP NUNC−IMMUNO MODULEプレート(NUNC社製)の1ウェルあたり 100μLずつ添加し、4℃にて1日以上感作させた。感作後、液を捨て、ブロッキング試薬{1重量%牛血清アルブミンF−V(ナカライテスク株式会社製)+リン酸緩衝液(pH7.2、0.1重量% NaN3含有)}を1ウェルあたり100μLずつ添加し、37℃、60分間反応させた。反応後、リン酸緩衝液(pH7.2、ツイーン20 0.1重量%含有)にてプレートを洗浄した。ペルオキシダーゼ標識したDerf2モノクローナル抗体を蒸留水に溶解し、それをリン酸緩衝液(pH7.2、牛血清アルブミン 1重量%及びツイーン20 0.1重量%含有)で10倍希釈した液を、1ウェルあたり100μLずつ添加した。37℃、60分間反応させた後、まずリン酸緩衝液(pH6.2)15mLに オルトフェニレンジアミン ジヒドロクロライド(30mg Tablet、SIGMA CHEMICAL CO.製)と30%過酸化水素水 15μLを加えたものを1ウェルあたり100μLずつ添加し、37℃、5分間反応させた。その後直ちに、2mol/L 硫酸水溶液を50μLずつ入れて反応を停止させ、マイクロプレート用分光光度計(Bio−Rad Laboratories Inc.製)で吸光度(OD490nm)を測定した。また、実施例及び比較例にて製剤したアレルゲン低減化剤を散布しない以外は同様に試験を行ったものをブランクとした。結果を表5に示した。
<Test Example 2> Measurement 2 of reduction effect of composition of the present invention against mite allergen Der 2
About 0.03 g of standard house dust (containing about 1000 μg / g of mite allergen Der 2) is placed on a polyester felt cut into a circular shape of 6.6 cm in diameter, and a trigger spray is used to put Examples 1-3. And about 2 g of the allergen-reducing composition of Comparative Example 1 and the same. After naturally drying at room temperature for 5 hours or more, put the felt in a vinyl bag with a chuck, add 10 ml of phosphate buffer (pH 7.0, containing 15% by weight of bovine serum albumin), and thoroughly extract the mite allergen. . The extract was centrifuged (12,000 rpm × 60 min), and the amount of mite allergen was measured by the sandwich method of enzyme immunoassay (ELISA method) using the supernatant. First, Derf2 monoclonal antibody 13A4 (1000 ng / 1 μL) was diluted 500-fold with a phosphate buffer (pH 7.4, containing 0.1 wt% NaN3), and 1 of F16 MAXISORP NUNC-IMMUNO MODULE plate (manufactured by NUNC) was used. 100 μL was added per well and sensitized at 4 ° C. for 1 day or longer. After sensitization, the solution is discarded, and blocking reagent {1 wt% bovine serum albumin F-V (manufactured by Nacalai Tesque) + phosphate buffer (pH 7.2, containing 0.1 wt% NaN3)} per well 100 μL was added and reacted at 37 ° C. for 60 minutes. After the reaction, the plate was washed with a phosphate buffer (pH 7.2, containing 0.1% by weight of Tween 20). A solution obtained by dissolving peroxidase-labeled Derf2 monoclonal antibody in distilled water and diluting it 10 times with phosphate buffer (pH 7.2, containing 1% by weight of bovine serum albumin and 0.1% by weight of Tween 20) was added to 1 well. 100 μL was added per unit. After reacting at 37 ° C. for 60 minutes, first, 15 mL of phosphate buffer (pH 6.2) to which orthophenylenediamine dihydrochloride (30 mg Tablet, manufactured by SIGMA CHEMICAL CO.) And 15 μL of 30% hydrogen peroxide solution were added. 100 μL was added per well and reacted at 37 ° C. for 5 minutes. Immediately thereafter, 50 μL each of 2 mol / L sulfuric acid aqueous solution was added to stop the reaction, and the absorbance (OD490 nm) was measured with a spectrophotometer for microplate (manufactured by Bio-Rad Laboratories Inc.). Moreover, what was similarly tested except not spraying the allergen reducing agent formulated in Examples and Comparative Examples was used as a blank. The results are shown in Table 5.
<試験例3> 抗アレルゲンフィルターによるアレルゲン低減化効果の測定
エアコンディショナー(ダイキン工業株式会社製)の樹脂製フィルターを1/3ずつに区切り、1/3を無処理とし、残りの2箇所の区画にそれぞれ実施例1と比較例1を5gずつ塗布し、常温で乾燥させた。このフィルターを装着し1日に約3時間、送風状態で7日間運転させた。ダストサンプラー(シントーファイン株式会社製)及びマイティフェルト(シントーファイン株式会社製)を装着した掃除機で、樹脂製フィルターの1/3ずつの区画をそれぞれのマイティフェルトに吸引した。ダストを採取したフェルトをチャック付きビニル袋に入れ、リン酸緩衝液(pH7.0、牛血清アルブミン 15重量%含有)10mLを加えてよく揉み、ダニアレルゲンを抽出した。抽出液を遠心分離器にかけ(12,000rpm×60min)、上澄み液のダニアレルゲン量の測定を試験例1と同様に行った。結果を表6に示した。
<Test Example 3> Measurement of allergen reduction effect by anti-allergen filter The resin filter of the air conditioner (Daikin Kogyo Co., Ltd.) was divided into 1/3 each, 1/3 was left untreated, and the remaining two compartments Each 5 g of Example 1 and Comparative Example 1 were applied and dried at room temperature. The filter was attached and operated for about 3 hours per day for 7 days in a blown state. With a vacuum cleaner equipped with a dust sampler (manufactured by Shinto Fine Co., Ltd.) and a mighty felt (manufactured by Shinto Fine Co., Ltd.), 1/3 each of the resin filter was sucked into each mighty felt. The felt from which the dust was collected was put into a vinyl bag with a chuck, and 10 mL of a phosphate buffer (pH 7.0, containing 15% by weight of bovine serum albumin) was added, and the mite allergen was extracted. The extract was centrifuged (12,000 rpm × 60 min), and the amount of mite allergen in the supernatant was measured in the same manner as in Test Example 1. The results are shown in Table 6.
本発明により、ダニや花粉等のアレルゲンのアレルゲン性を低減化させることができる。また、フィルターまたはシートにアレルゲン性を低減化させる機能を付与するためのアレルゲン低減化組成物、およびアレルゲンを低減化できるフィルターまたはシートを提供することができる。 According to the present invention, the allergenicity of allergens such as mites and pollen can be reduced. Moreover, the filter or sheet | seat which can reduce the allergen and the allergen reduction composition for providing the function to reduce allergenicity to a filter or a sheet | seat can be provided.
Claims (3)
A mite allergen-reducing filter or sheet obtained by processing the mite allergen-reducing composition according to claim 1.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2006268797A JP5361123B2 (en) | 2006-09-29 | 2006-09-29 | Allergen reducing composition and allergen reducing method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2006268797A JP5361123B2 (en) | 2006-09-29 | 2006-09-29 | Allergen reducing composition and allergen reducing method |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2008088232A JP2008088232A (en) | 2008-04-17 |
JP5361123B2 true JP5361123B2 (en) | 2013-12-04 |
Family
ID=39372719
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006268797A Expired - Fee Related JP5361123B2 (en) | 2006-09-29 | 2006-09-29 | Allergen reducing composition and allergen reducing method |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP5361123B2 (en) |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4342120B2 (en) * | 2001-04-27 | 2009-10-14 | 住化エンビロサイエンス株式会社 | Anti-allergen composition and method for inactivating allergen |
US7037535B2 (en) * | 2002-11-19 | 2006-05-02 | Kimberly-Clark Worldwide, Inc. | Method and composition for neutralizing house dust mite feces |
JP2005290331A (en) * | 2004-04-06 | 2005-10-20 | Shinto Fine Co Ltd | Antiallergenic composition and method for deactivating allergen |
JP4845349B2 (en) * | 2004-06-02 | 2011-12-28 | 大和化学工業株式会社 | Mite allergen deactivator |
JP4731141B2 (en) * | 2004-08-31 | 2011-07-20 | 住化エンビロサイエンス株式会社 | Anti-allergen composition and method for inactivating allergen |
JP4818612B2 (en) * | 2005-01-11 | 2011-11-16 | ユニ・チャーム株式会社 | Disposable mask |
JP4896409B2 (en) * | 2005-01-28 | 2012-03-14 | 花王株式会社 | Pollen allergy symptom reducing composition |
-
2006
- 2006-09-29 JP JP2006268797A patent/JP5361123B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
JP2008088232A (en) | 2008-04-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP4342120B2 (en) | Anti-allergen composition and method for inactivating allergen | |
EP1588710B9 (en) | Anti-allergic composition and method for denaturing allergens | |
JP4968899B2 (en) | Anti-allergen composition | |
JP4766639B2 (en) | Anti-allergen composition and allergen inactivation method | |
KR20080005510A (en) | Allergen inactivator | |
JP4716466B2 (en) | Anti-allergen composition and allergen inactivation method | |
JP5114692B2 (en) | Anti-allergen composition and method for reducing allergen | |
JP2006265754A (en) | Anti-allergenic composition | |
JP2010116450A (en) | Antiallergenic composition | |
DE60104578T2 (en) | Process for denaturing allergens | |
JP4421127B2 (en) | Anti-allergen composition and allergen inactivation method | |
JP5361123B2 (en) | Allergen reducing composition and allergen reducing method | |
WO2015141712A1 (en) | Allergen-reducing composition, spray agent and surface treating agent containing same, and allergen-reducing method | |
JP2005273099A (en) | Anti-allergen textile product | |
JP2006335934A (en) | Anti-allergen composition and allergen inactivation method | |
JP5295480B2 (en) | Anti-allergen composition | |
JP4780648B2 (en) | Anti-allergen composition | |
JP4731141B2 (en) | Anti-allergen composition and method for inactivating allergen | |
JP2007056394A (en) | Anti-allergenic composition | |
JP5467636B2 (en) | Anti-allergen composition | |
JP4823615B2 (en) | Allergen-reducing composition | |
JP2007077557A (en) | Textile fiber product for antiallergenic cleaning use | |
US8338404B2 (en) | Composition and method for reducing allergen | |
JP2006328108A (en) | Anti-allergic composition | |
JP2020164604A (en) | Allergen-reducing composition |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20090512 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20120104 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20120118 |
|
A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20120202 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20130614 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20130801 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20130903 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 5361123 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313111 |
|
R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
LAPS | Cancellation because of no payment of annual fees |