JP2001226208A - Microbiocidal composition - Google Patents
Microbiocidal compositionInfo
- Publication number
- JP2001226208A JP2001226208A JP2000041092A JP2000041092A JP2001226208A JP 2001226208 A JP2001226208 A JP 2001226208A JP 2000041092 A JP2000041092 A JP 2000041092A JP 2000041092 A JP2000041092 A JP 2000041092A JP 2001226208 A JP2001226208 A JP 2001226208A
- Authority
- JP
- Japan
- Prior art keywords
- hypochlorite
- aqueous solution
- salt
- product
- amine oxide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は殺菌剤組成物に関す
る。[0001] The present invention relates to a fungicide composition.
【０００２】[0002]
【従来の技術及び発明が解決しようとする課題】従来、
広範な環境における殺菌消毒剤として、次亜塩素酸ナト
リウム、次亜塩素酸カルシウム、ジクロロイソシアヌル
酸ナトリウム等の塩素系殺菌剤が広く用いられている。
中でも次亜塩素酸ナトリウム等の次亜塩素酸塩は、価格
面と効果の点で汎用されているが、医療、食品工業等、
種々の分野で要求される微生物の殺菌、滅菌に対して、
更にその効力を向上させるための多くの提案がなされて
いる。例えば、特開昭５７−６１０９９号には、次亜塩
素酸塩、アルカリ性物質及び特定の第四級アンモニウム
塩型カチオン界面活性剤を、それぞれ特定の重量比で含
有する液体殺菌漂白剤組成物が、特開平７−２３３３９
６号には、次亜塩素酸塩、陰イオン界面活性剤、アルカ
リ剤及びキレート剤を含有する人工透析機等の医療機器
用の殺菌洗浄剤が開示されている。2. Description of the Related Art
As a disinfectant in a wide range of environments, chlorine-based disinfectants such as sodium hypochlorite, calcium hypochlorite, sodium dichloroisocyanurate and the like are widely used.
Above all, hypochlorites such as sodium hypochlorite are widely used in terms of price and effect, but medical, food industry, etc.
For sterilization and sterilization of microorganisms required in various fields,
Many proposals have been made to further improve its efficacy. For example, JP-A-57-61099 discloses a liquid germicidal bleach composition containing hypochlorite, an alkaline substance and a specific quaternary ammonium salt type cationic surfactant in a specific weight ratio. JP-A-7-23339
No. 6 discloses a germicidal detergent for medical equipment such as an artificial dialysis machine containing a hypochlorite, an anionic surfactant, an alkali agent and a chelating agent.
【０００３】しかしながら、従来の次亜塩素酸塩系殺菌
剤は、一般細菌やカビ（菌糸）についてはある程度の効
果があるものの、薬品耐性のより高いウイルス、桿菌の
形成する芽胞、カビ胞子に対しては簡易な操作では十分
な効果が期待できない。[0003] However, conventional hypochlorite fungicides, although effective to some extent against common bacteria and mold (hyphae), are effective against viruses with higher drug resistance, spores and fungal spores formed by bacilli. In other words, a simple operation cannot expect a sufficient effect.
【０００４】また、特開平１１−１４８０９８号には高
度さらし粉（次亜塩素酸カルシウム）のような次亜塩素
酸アルカリ土類金属塩を用いた固形殺菌洗浄剤が開示さ
れているが、より高度の殺菌処理について認識されてお
らず、しかもカルシウム等のアルカリ土類金属がスケー
ル、スカムの発生原因となり、殺菌効率の低下をもたら
す。Japanese Unexamined Patent Application Publication No. 11-148098 discloses a solid germicidal detergent using an alkaline earth metal hypochlorite such as highly bleached powder (calcium hypochlorite). Is not recognized, and alkaline earth metals such as calcium cause scale and scum, resulting in lower sterilization efficiency.
【０００５】なお、特開昭５９−９３７９９号には、ア
ミンオキサイドを、次亜塩素酸塩を含有する液体洗浄剤
に配合することが、また、特開昭５９−９８２００号に
は、アミンオキサイドを、次亜塩素酸塩を含有する漂白
剤の増粘剤として用いることが開示されているが、これ
らには殺菌、特に耐性の高い芽胞やウイルスの殺菌に関
しての言及はない。JP-A-59-93799 discloses that an amine oxide is incorporated into a liquid detergent containing hypochlorite, and JP-A-59-98200 discloses an amine oxide. Is disclosed as a thickener for bleach containing hypochlorite, but there is no mention of disinfection, in particular disinfection of highly resistant spores and viruses.
【０００６】本発明は、簡易な処理により、高い殺菌効
果が得られ、且つ安全性、作業性に優れた殺菌剤組成物
を得ることを目的とする。An object of the present invention is to obtain a bactericide composition which can provide a high bactericidal effect by simple processing, and is excellent in safety and workability.
【０００７】[0007]
【課題を解決するための手段】本発明は、次亜塩素酸塩
（Ａ）とアミンオキサイド（Ｂ）とを、（Ａ）／（Ｂ）
＝１０／１〜１／１０の重量比で含有する殺菌剤組成
物、更に有機酸又はその塩（Ｃ）を含有する該殺菌剤組
成物に関する。According to the present invention, a hypochlorite (A) and an amine oxide (B) are combined with (A) / (B)
The present invention relates to a bactericide composition containing the bactericide composition at a weight ratio of 10/1 to 1/10, and further containing the organic acid or its salt (C).
【０００８】また、本発明は、次亜塩素酸塩（Ａ）を含
む成分の製品（Ｉ）と、アミンオキサイド（Ｂ）を含む
成分の製品（II）とからなり、使用時に（Ａ）／（Ｂ）
＝１０／１〜１／１０の重量比で含有する水溶液として
用いられる殺菌剤に関する。Further, the present invention comprises a product (I) containing a component containing hypochlorite (A) and a product (II) containing a component containing an amine oxide (B). (B)
The present invention relates to a disinfectant used as an aqueous solution containing a weight ratio of 10/1 to 1/10.
【０００９】また、本発明は、次亜塩素酸塩（Ａ）を含
む成分の製品（III）とアミンオキサイド（Ｂ）と有機
酸又はその塩（Ｃ）を含む成分の製品（IV）とからな
り、使用時に（Ａ）／（Ｂ）＝１０／１〜１／１０の重
量比で含有する水溶液として用いられる殺菌剤に関す
る。Further, the present invention relates to a product (III) containing a component containing hypochlorite (A), a product (IV) containing a component containing an amine oxide (B) and an organic acid or a salt thereof (C). The present invention relates to a disinfectant used as an aqueous solution containing (A) / (B) at a weight ratio of 10/1 to 1/10 when used.
【００１０】更に本発明は、次亜塩素酸塩（Ａ）とアミ
ンオキサイド（Ｂ）とを含む成分の製品（Ｖ）と、有機
酸又はその塩（Ｃ）を含む成分の製品（VI）とからな
り、使用時に（Ａ）／（Ｂ）＝１０／１〜１／１０の重
量比で含有する水溶液として用いられる殺菌剤に関す
る。The present invention further relates to a product (V) containing a component containing hypochlorite (A) and an amine oxide (B), and a product (VI) containing a component containing an organic acid or a salt thereof (C). And a disinfectant used when used as an aqueous solution containing (A) / (B) at a weight ratio of 10/1 to 1/10.
【００１１】[0011]
【発明の実施の形態】本発明に用いられる次亜塩素酸塩
（Ａ）としては、次亜塩素酸ナトリウム、次亜塩素酸カ
リウム、次亜塩素酸リチウム等が挙げられるが、次亜塩
素酸ナトリウムが好ましい。BEST MODE FOR CARRYING OUT THE INVENTION The hypochlorite (A) used in the present invention includes sodium hypochlorite, potassium hypochlorite, lithium hypochlorite and the like. Sodium is preferred.
【００１２】本発明に用いられるアミンオキサイド
（Ｂ）としては、アルキルジメチルアミンオキサイドが
挙げられ、特に炭素数８〜１８のアルキル基を有するも
のが好ましい。Examples of the amine oxide (B) used in the present invention include alkyldimethylamine oxides, and particularly those having an alkyl group having 8 to 18 carbon atoms are preferred.
【００１３】本発明の殺菌剤組成物は、次亜塩素酸塩
（Ａ）とアミンオキサイド（Ｂ）とを、（Ａ）／（Ｂ）
＝１０／１〜１／１０、好ましくは５／１〜１／５、よ
り好ましくは２／１〜１／２の重量比で含有する。[0013] The fungicide composition of the present invention comprises a mixture of hypochlorite (A) and amine oxide (B), (A) / (B).
= 10/1 to 1/10, preferably 5/1 to 1/5, more preferably 2/1 to 1/2.
【００１４】ただし、（Ａ）成分は有効塩素基準での重
量を示す。However, the component (A) indicates a weight based on available chlorine.
【００１５】本発明の殺菌剤組成物は、殺菌性能向上の
観点から、更に有機酸又はその塩（Ｃ）を含有すること
が好ましい。有機酸又はその塩（Ｃ）としては、マロン
酸、コハク酸、グルタル酸、アジピン酸、セバシン酸等
の飽和二塩基酸又はその塩や、フマル酸、マレイン酸等
の不飽和二塩基酸又はその塩等が挙げられる。好ましく
は飽和二塩基酸又はその塩、より好ましくは炭素数３〜
１０の飽和二塩基酸又はその塩であり、特にコハク酸又
はその塩が好ましい。有機酸又はその塩（Ｃ）は、次亜
塩素酸塩（Ａ）との重量比が（Ｃ）／（Ａ）＝５／１〜
１／１０、更に２／１〜１／５、特に１／１〜１／５と
なるように用いられるのが好ましい。The germicidal composition of the present invention preferably further contains an organic acid or a salt thereof (C) from the viewpoint of improving bactericidal performance. Examples of the organic acid or a salt thereof (C) include a saturated dibasic acid such as malonic acid, succinic acid, glutaric acid, adipic acid and sebacic acid or a salt thereof, and an unsaturated dibasic acid such as fumaric acid and maleic acid or a salt thereof. And the like. Preferably a saturated dibasic acid or a salt thereof, more preferably 3 to 3 carbon atoms
And a saturated dibasic acid or a salt thereof, particularly preferably succinic acid or a salt thereof. The weight ratio of the organic acid or the salt thereof (C) to the hypochlorite (A) is (C) / (A) = 5/1 to 1.
It is preferably used so as to be 1/10, more preferably 2/1 to 1/5, especially 1/1 to 1/5.
【００１６】本発明の殺菌剤組成物は、アルカリ金属の
水酸化物及び／又はアルカリ土類金属の水酸化物（Ｄ）
を含有してもよい。（Ｄ）としては、例えば水酸化ナト
リウム、水酸化カリウム、水酸化カルシウム等が挙げら
れ、水酸化ナトリウム、水酸化カリウムが好ましい。The fungicide composition of the present invention comprises a hydroxide of an alkali metal and / or a hydroxide of an alkaline earth metal (D).
May be contained. Examples of (D) include sodium hydroxide, potassium hydroxide, calcium hydroxide and the like, and sodium hydroxide and potassium hydroxide are preferred.
【００１７】また、本発明の殺菌剤組成物は、無機酸の
アルカリ金属塩及び／又は無機酸のアルカリ土類金属塩
（Ｅ）を含有してもよい。（Ｅ）成分としては硫酸ナト
リウム、硝酸ナトリウム、塩化ナトリウム、炭酸ナトリ
ウム、炭酸水素ナトリウム、硫酸マグネシウム、硝酸マ
グネシウム、塩化マグネシウム、炭酸マグネシウム、リ
ン酸ナトリウム、ポリリン酸ナトリウム、リン酸カリウ
ム等が挙げられ、硫酸ナトリウム、硫酸マグネシウム、
リン酸ナトリウム、ポリリン酸ナトリウム、リン酸カリ
ウムが好ましい。The bactericide composition of the present invention may contain an alkali metal salt of an inorganic acid and / or an alkaline earth metal salt of an inorganic acid (E). Examples of the component (E) include sodium sulfate, sodium nitrate, sodium chloride, sodium carbonate, sodium hydrogen carbonate, magnesium sulfate, magnesium nitrate, magnesium chloride, magnesium carbonate, sodium phosphate, sodium polyphosphate, potassium phosphate, and the like. Sodium sulfate, magnesium sulfate,
Sodium phosphate, sodium polyphosphate and potassium phosphate are preferred.
【００１８】本発明の殺菌剤組成物は、上記（Ａ）成分
と（Ｂ）成分とを特定重量比で含有する水溶液からな
る。この水溶液の有効塩素濃度は５〜５０００ｐｐｍ、
特に５０〜２００ｐｐｍが好ましい。また、水溶液のｐ
Ｈ（２５℃）は好ましくは５〜１２、より好ましくは５
〜１０、更に好ましくは５〜８、特に好ましくは５以上
７未満、更に特に好ましくは６以上７未満である。水溶
液のｐＨの調整は上記有機酸又はその塩（Ｃ）や無機酸
により行うことができる。また、本発明の殺菌剤組成物
となる水溶液中の各成分の量は、（Ａ）成分が５ｐｐｍ
〜１２重量％、（Ｂ）成分が０．５ｐｐｍ〜３５重量％
が好ましく、（Ｃ）成分を配合する場合は、０．５ｐｐ
ｍ〜６０重量％が好ましい。使用にあたっては、この水
溶液を更に希釈した水溶液が用いられるが、この希釈水
溶液は、（Ａ）成分を５〜５０００ｐｐｍ、更に１０〜
５０００ｐｐｍ、特に５０〜２００ｐｐｍ、（Ｂ）成分
を０．５〜５００００ｐｐｍ、更に５〜２０００ｐｐ
ｍ、特に５０〜２００ｐｐｍ、（Ｃ）成分を０．５〜２
５０００ｐｐｍ、更に５〜１０００ｐｐｍ、更にまた２
５〜５００ｐｐｍ、特に２５〜１５０ｐｐｍ含有するこ
とが好ましい。なお、本発明の殺菌剤組成物の対象とな
る微生物とは、一般細菌、糸状菌、ウイルス、カビ胞
子、細菌芽胞等を意味する。The germicidal composition of the present invention comprises an aqueous solution containing the above components (A) and (B) at a specific weight ratio. The effective chlorine concentration of this aqueous solution is 5 to 5000 ppm,
In particular, 50 to 200 ppm is preferable. In addition, p of the aqueous solution
H (25 ° C.) is preferably 5 to 12, more preferably 5 to 12.
It is preferably from 10 to 10, more preferably from 5 to 8, particularly preferably from 5 to less than 7, more preferably from 6 to less than 7. The pH of the aqueous solution can be adjusted with the above organic acid or its salt (C) or inorganic acid. In addition, the amount of each component in the aqueous solution to be the fungicide composition of the present invention is such that the component (A) is 5 ppm.
-12% by weight, component (B) 0.5ppm-35% by weight
When the component (C) is blended, 0.5 pp
m to 60% by weight is preferred. In use, an aqueous solution obtained by further diluting the aqueous solution is used. The diluted aqueous solution contains the component (A) in an amount of 5 to 5000 ppm,
5000 ppm, especially 50 to 200 ppm, component (B) 0.5 to 50000 ppm, and further 5 to 2000 pp
m, especially 50 to 200 ppm, and the component (C) is 0.5 to 2
5000 ppm, 5 to 1000 ppm, and 2
The content is preferably 5 to 500 ppm, particularly preferably 25 to 150 ppm. The microorganism to be used in the fungicide composition of the present invention means general bacteria, filamentous fungi, viruses, mold spores, bacterial spores and the like.
【００１９】本発明の殺菌剤組成物は、殺菌スペクトル
が広く、細菌（カビ）のみならず、ウイルスや芽胞に対
する効果も高いため、幅広い分野での殺菌に有用であ
る。例えば、病院、養護施設、食品加工工場、クリーニ
ング施設、厨房等の壁、床、窓等あるいはそれらで用い
られる器具、備品、及び製品用（例えば飲料液用）容器
等の殺菌に用いられる。The germicidal composition of the present invention has a broad bactericidal spectrum and is highly effective against not only bacteria (mold) but also viruses and spores, and is therefore useful for bactericidal in a wide range of fields. For example, it is used for sterilization of walls, floors, windows, etc. of hospitals, nursing homes, food processing factories, cleaning facilities, kitchens and the like, or instruments, fixtures, and products (for example, beverages) containers used therein.
【００２０】本発明の殺菌剤は、次亜塩素酸塩（Ａ）を
含む成分の製品（Ｉ）とアミンオキサイド（Ｂ）を含む
成分の製品（II）とからなり、使用時に（Ａ）／（Ｂ）
＝１０／１〜１／１０の重量比で含有する水溶液として
用いられる。それぞれの製品には安定性等を損なわない
限り任意成分を含んでいても良い。また、製品（Ｉ）、
（II）と、更にこれら以外の製品により構成されていて
もよい。The disinfectant of the present invention comprises a product (I) containing a component containing hypochlorite (A) and a product (II) containing a component containing an amine oxide (B). (B)
= 10/1 to 1/10 in weight ratio. Each product may contain optional components as long as stability and the like are not impaired. In addition, product (I),
It may be composed of (II) and other products.
【００２１】また、有機酸又はその塩（Ｃ）を用いる場
合は、次亜塩素酸塩（Ａ）とは別に配合することが好ま
しい。その具体例として、次亜塩素酸塩（Ａ）を含む成
分の製品（III）とアミンオキサイド（Ｂ）と有機酸又
はその塩（Ｃ）を含む成分の製品（IV）とからなり、使
用時に（Ａ）／（Ｂ）＝１０／１〜１／１０の重量比で
含有する水溶液として用いられる殺菌剤が挙げられる。
この殺菌剤は、使用時に（Ａ）成分と（Ｃ）成分を、
（Ｃ）／（Ａ）＝５／１〜１／１０、更に２／１〜１／
５、特に１／１〜１／５の重量比で含有する水溶液とし
て用いられるのが好ましい。また、次亜塩素酸塩（Ａ）
とアミンオキサイド（Ｂ）とを含む成分の製品（Ｖ）
と、有機酸又はその塩（Ｃ）を含む成分の製品（VI）と
からなり、使用時に（Ａ）／（Ｂ）＝１０／１〜１／１
０の重量比で含有する水溶液として用いられる殺菌剤が
挙げられる。この殺菌剤は、使用時に（Ａ）成分と
（Ｃ）成分を、（Ｃ）／（Ａ）＝５／１〜１／１０、更
に２／１〜１／５、特に１／１〜１／５の重量比で含有
する水溶液として用いられるのが好ましい。それぞれの
製品には安定性等を損なわない限り任意成分を含んでい
ても良い。また、製品（III）〜（VI）と、更にこれら
以外の製品により構成されていてもよい。When an organic acid or its salt (C) is used, it is preferable to mix it separately from the hypochlorite (A). Specific examples thereof include a product (III) of a component containing hypochlorite (A), a product (IV) of a component containing an amine oxide (B) and an organic acid or a salt thereof (C). A bactericide used as an aqueous solution containing (A) / (B) at a weight ratio of 10/1 to 1/10 is exemplified.
This disinfectant, when used, (A) component and (C) component,
(C) / (A) = 5/1 to 1/10, further 2/1 to 1 /
It is preferably used as an aqueous solution containing 5, especially at a weight ratio of 1/1 to 1/5. In addition, hypochlorite (A)
(V), which is a component containing an amine oxide and an amine oxide (B)
And a product (VI) containing an organic acid or a salt thereof (C), and (A) / (B) = 10/1 to 1/1 when used.
A bactericide used as an aqueous solution containing at a weight ratio of 0 may be mentioned. This disinfectant mixes (A) component and (C) component at the time of use, (C) / (A) = 5/1 to 1/10, further 2/1 to 1/5, particularly 1/1 to 1 / It is preferably used as an aqueous solution containing at a weight ratio of 5. Each product may contain optional components as long as stability and the like are not impaired. Further, it may be constituted by products (III) to (VI) and further products other than these.
【００２２】製品（Ｉ）〜（VI）の形態は限定されず、
好ましくはガラス、金属、プラスチック、紙等の材質を
用いた種々の包装容器に各成分を充填したものである。
また、製品（Ｉ）〜（VI）の内容物はそのままでも、あ
るいは希釈して用いることができる。各製品中の有効分
の濃度は、製品（Ｉ）では次亜塩素酸塩が０．１〜１２
重量％、（II）ではアミンオキサイドが０．１〜３５重
量％、（III）では次亜塩素酸塩が０．１〜１２重量
％、（IV）ではアミンオキサイドと有機酸又はその塩と
の合計が０．２〜６０重量％、（Ｖ）では次亜塩素酸塩
とアミンオキサイドの合計が０．２〜４７重量％、（V
I）では有機酸又はその塩が０．１〜６０重量％が好ま
しい。The forms of the products (I) to (VI) are not limited.
Preferably, various components are filled in various packaging containers using materials such as glass, metal, plastic, and paper.
Further, the contents of the products (I) to (VI) can be used as they are or diluted. The concentration of the active ingredient in each product is 0.1 to 12% for hypochlorite in product (I).
% By weight, (II) 0.1 to 35% by weight of amine oxide, (III) 0.1 to 12% by weight of hypochlorite, (IV) of amine oxide and organic acid or salt thereof. In the case of (V), the total amount of hypochlorite and amine oxide is 0.2 to 47% by weight,
In (I), the organic acid or its salt is preferably 0.1 to 60% by weight.
【００２３】これら、製品（Ｉ）と（II）、又は製品
（III）と（IV）、又は製品（Ｖ）と（VI）とから本発
明の殺菌剤組成物を調製することができる。The fungicide composition of the present invention can be prepared from these products (I) and (II), or products (III) and (IV), or products (V) and (VI).
【００２４】[0024]
【発明の効果】本発明によれば、殺菌効果が高く、且つ
安全性、作業性に優れた殺菌剤組成物が得られる。According to the present invention, a bactericidal composition having a high bactericidal effect and excellent in safety and workability can be obtained.
【００２５】[0025]
【実施例】実施例１〜６及び比較例１〜４ 表１に示す組成の成分からなる水溶液を用いて、以下の
試験を行った。結果を表１に示す。EXAMPLES Examples 1 to 6 and Comparative Examples 1 to 4 The following tests were carried out using aqueous solutions composed of the components shown in Table 1. Table 1 shows the results.
【００２６】なお、実施例１の配合成分は、次亜塩素酸
ナトリウム水溶液（有効塩素濃度６００００ｐｐｍ）と
アミンオキサイド（有効分３５％）を用い、それぞれ最
終配合濃度の２倍までイオン交換水で希釈したものを等
量混合して得たものである。また、実施例２〜４の配合
成分は、次亜塩素酸ナトリウム水溶液（有効塩素濃度６
００００ｐｐｍ）とアミンオキサイドを所定量混合し得
られたものを最終配合濃度の２倍までイオン交換水で希
釈したものとコハク酸を最終配合濃度の２倍までイオン
交換水で希釈したものを等量混合して得たものである。
また、実施例５、６の配合成分は、次亜塩素酸ナトリウ
ム水溶液（有効塩素濃度６００００ｐｐｍ）を最終濃度
の２倍までイオン交換水で希釈したものと、アミンオキ
サイドとコハク酸を所定量混合し得られたものを最終配
合濃度の２倍までイオン交換水で希釈したものとを等量
混合して得たものである。The components of Example 1 were diluted with ion-exchanged water to twice the final compounding concentration using an aqueous solution of sodium hypochlorite (effective chlorine concentration: 60000 ppm) and amine oxide (effective component: 35%). It was obtained by mixing equal amounts of the above. In addition, the compounding components of Examples 2 to 4 were sodium hypochlorite aqueous solution (effective chlorine concentration of 6
0000 ppm) and a predetermined amount of amine oxide were mixed and diluted with ion-exchanged water to twice the final blending concentration, and succinic acid was diluted with ion-exchanged water to twice the final blending concentration. It was obtained by mixing.
The components of Examples 5 and 6 were prepared by diluting an aqueous solution of sodium hypochlorite (effective chlorine concentration: 60000 ppm) with ion-exchanged water to twice the final concentration, and mixing a predetermined amount of amine oxide and succinic acid. It was obtained by mixing equal amounts of the obtained product diluted with ion-exchanged water to twice the final blending concentration.
【００２７】〔１〕殺芽胞試験 芽胞菌として、セレウス菌（Bacillus cereus IFO13
494）、枯草菌（Bacillus subtilis ATCC6051）を用
い、定法により熱処理を行い、得られた芽胞を試験に供
した。すなわち、ＳＣＤ寒天培地（日本製薬（株）製）
に前培養した菌を一白金耳かきとり、１ｍｌの滅菌水に
懸濁し、６５℃、３０分間の熱処理後、２回遠心分離洗
浄を行ったものを試験に用いた。[1] Spore killing test Bacillus cereus IFO13
494) and heat treatment was carried out using Bacillus subtilis ATCC6051 by a standard method, and the obtained spores were subjected to a test. That is, SCD agar medium (manufactured by Nippon Pharmaceutical Co., Ltd.)
The bacteria pre-cultured were scraped with a platinum loop, suspended in 1 ml of sterilized water, heat-treated at 65 ° C. for 30 minutes, and washed twice by centrifugation, and used for the test.
【００２８】この試験用芽胞菌液（約１０9〜１０10cel
l／ｍｌ）を０．１ｍｌとり、表１の成分からなる水溶
液を更に滅菌したイオン交換水で稀釈した水溶液（温度
２５℃）１０ｍｌに１０秒間接触させた後、５０μｌを
採取し、後培養用ＳＣＤＬＰ培地（チオ硫酸ナトリウム
３．３％含有）０．２ｍｌの入ったミクロシャーレ（CO
RNING社製、96-Cell Wells）へ接種した。３０℃で４
８時間培養し、菌の発育を肉眼で観察し、ミクロシャー
レ上で菌が生育しているかどうかを観察し、菌の生育が
ない（つまり１００％殺菌できる）最小の希釈倍率（最
小殺菌有効塩素濃度）を求めた。なお、有効塩素濃度
は、ＪＩＳ Ｋ−０１０１“ヨウ素法”により測定した
ものである。This test spore bacterium solution (about 10 9 to 10 10 cel)
l / ml), contact the aqueous solution comprising the components shown in Table 1 with 10 ml of an aqueous solution (temperature 25 ° C.) further diluted with sterilized ion-exchanged water for 10 seconds, and collect 50 μl of the solution for post-culture. A micro Petri dish containing 0.2 ml of SCDLP medium (containing 3.3% of sodium thiosulfate) (CO
RNING, 96-Cell Wells). 4 at 30 ° C
After culturing for 8 hours, the growth of the bacteria is observed with the naked eye, and whether or not the bacteria are growing on a micro Petri dish is observed. Concentration). The effective chlorine concentration is measured according to JIS K-0101 “Iodine method”.
【００２９】〔２〕殺カビ試験 被験菌としてカビ（真菌、Aspergillus niger IFO634
1）は、ＰＤＡ培地を用い、２５℃で７日間培養した。
得られた菌体をガラス玉法を用い、均一にした後、滅菌
ガーゼで異物を除去し、菌液を得た。この菌液（約１０
9〜１０10cell／ｍｌ）を０．１ｍｌとり、表１の成分
からなる水溶液を更に滅菌したイオン交換水で表１に示
す倍率で稀釈した水溶液（温度２５℃）１０ｍｌに１０
秒間接触させた後、０．１ｍｌを採取し、後培養用ＰＤ
Ａ培地（チオ硫酸ナトリウム３．３％含有）へ接種し
た。２５℃で７時間培養し、菌の発育を肉眼で観察し、
上記同様に評価した。[2] Mold killing test As a test bacterium, a mold (fungus, Aspergillus niger IFO634) was used.
1) was cultured for 7 days at 25 ° C. using a PDA medium.
After the obtained cells were homogenized by a glass ball method, foreign substances were removed with sterile gauze to obtain a bacterial solution. This bacterial solution (about 10
0.1 to 10 ml of an aqueous solution ( 9 to 10 10 cell / ml) was diluted with sterilized ion-exchanged water at the magnification shown in Table 1 to 10 ml of an aqueous solution (temperature 25 ° C.).
After contact for 2 seconds, 0.1 ml was collected and used for post-culture PD.
A medium (containing 3.3% sodium thiosulfate) was inoculated. Incubate at 25 ° C for 7 hours, observe the growth of the bacteria with the naked eye,
Evaluation was performed in the same manner as described above.
【００３０】[0030]
【表１】 [Table 1]
【００３１】実施例７〜１０ 表２の成分からなる水溶液をイオン交換水で表２に示す
倍率で希釈した試験用水溶液（有効塩素濃度２００ｐｐ
ｍ）を調製し、サンプルビンに密閉後、４０℃の恒温槽
で２日間保存した。なお、この水溶液は、次亜塩素酸ナ
トリウム水溶液（有効塩素濃度６００００ｐｐｍ）を最
終配合濃度の２倍までイオン交換水で希釈したものと、
アミンオキサイドと有機酸とを所定量混合し得られたも
のを最終配合濃度の２倍までイオン交換水で希釈したも
のを等量混合して得たものである。Examples 7 to 10 A test aqueous solution (effective chlorine concentration: 200 pp) was prepared by diluting an aqueous solution comprising the components shown in Table 2 with ion-exchanged water at the magnification shown in Table 2.
m) was prepared, sealed in a sample bottle, and stored in a thermostat at 40 ° C. for 2 days. In addition, this aqueous solution was prepared by diluting an aqueous solution of sodium hypochlorite (effective chlorine concentration: 60000 ppm) with ion-exchanged water to twice the final compounding concentration,
A mixture obtained by mixing a predetermined amount of an amine oxide and an organic acid, diluted with ion-exchanged water to twice the final compounding concentration, and then mixed in equal amounts.
【００３２】２日後にサンプルビンを取り出し、試験用
水溶液の有効塩素濃度を測定し、有効塩素濃度の保持率
（％）を次の式により求めた。結果を表２に示す。 保持率（％）＝〔４０℃で２日保存後の有効塩素濃度／
２００〕×１００Two days later, the sample bottle was taken out, the effective chlorine concentration of the test aqueous solution was measured, and the retention (%) of the effective chlorine concentration was determined by the following equation. Table 2 shows the results. Retention (%) = [effective chlorine concentration after storage at 40 ° C for 2 days /
200] × 100
【００３３】[0033]
【表２】 [Table 2]
───────────────────────────────────────────────────── フロントページの続き (72)発明者 岡野 哲也 和歌山県和歌山市湊1334 花王株式会社研 究所内 (72)発明者 田村 成 和歌山県和歌山市湊1334 花王株式会社研 究所内 Ｆターム(参考） 4H003 AB29 AC15 BA14 DA20 EA03 EB07 ED02 EE08 FA01 FA34 4H011 AA02 AA03 AA04 BA01 BA02 BA06 BB04 BB18 BC06 BC18 DA13 DF06 DG05 ──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Tetsuya Okano 1334 Minato, Wakayama-shi, Wakayama Pref. Inside the Kao Research Institute (72) Inventor Shigeru Tamura 1334 Minato, Wakayama-shi, Wakayama Pref. 4H003 AB29 AC15 BA14 DA20 EA03 EB07 ED02 EE08 FA01 FA34 4H011 AA02 AA03 AA04 BA01 BA02 BA06 BB04 BB18 BC06 BC18 DA13 DF06 DG05
Claims (7)
（Ｂ）とを、（Ａ）／（Ｂ）＝１０／１〜１／１０の重
量比で含有する水溶液からなる液状殺菌剤組成物。1. A liquid disinfectant composition comprising an aqueous solution containing a hypochlorite (A) and an amine oxide (B) in a weight ratio of (A) / (B) = 10/1 to 1/10. object.
項１記載の殺菌剤組成物。2. The fungicidal composition according to claim 1, comprising an organic acid or a salt thereof (C).
酸又はその塩である請求項２記載の殺菌剤組成物。3. The fungicidal composition according to claim 2, wherein the organic acid or a salt thereof (C) is a saturated dibasic acid or a salt thereof.
（Ａ）との重量比が（Ｃ）／（Ａ）＝５／１〜１／１０
である請求項２又は３記載の殺菌剤組成物。4. The weight ratio of the organic acid or its salt (C) to the hypochlorite (A) is (C) / (A) = 5/1 to 1/10.
The fungicide composition according to claim 2 or 3, wherein
（Ｉ）と、アミンオキサイド（Ｂ）を含む成分の製品
（II）とからなり、使用時に（Ａ）／（Ｂ）＝１０／１
〜１／１０の重量比で含有する水溶液として用いられる
殺菌剤。5. A product (I) containing a component containing hypochlorite (A) and a product (II) containing a component containing an amine oxide (B), wherein (A) / (B) = 10/1
A fungicide used as an aqueous solution containing a weight ratio of １／1/10.
（III）とアミンオキサイド（Ｂ）と有機酸又はその塩
（Ｃ）を含む成分の製品（IV）とからなり、使用時に
（Ａ）／（Ｂ）＝１０／１〜１／１０の重量比で含有す
る水溶液として用いられる殺菌剤。6. A product (III) containing a component containing hypochlorite (A), a product (IV) containing a component containing an amine oxide (B) and an organic acid or a salt thereof (C), A fungicide used as an aqueous solution containing (A) / (B) at a weight ratio of 10/1 to 1/10.
（Ｂ）とを含む成分の製品（Ｖ）と、有機酸又はその塩
（Ｃ）を含む成分の製品（VI）とからなり、使用時に
（Ａ）／（Ｂ）＝１０／１〜１／１０の重量比で含有す
る水溶液として用いられる殺菌剤。7. A product (V) of a component containing hypochlorite (A) and an amine oxide (B), and a product (VI) of a component containing an organic acid or a salt thereof (C), A disinfectant used as an aqueous solution containing (A) / (B) at a weight ratio of 10/1 to 1/10 when used.
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|---|---|---|---|
| JP2000041092A JP3607555B2 (en) | 1999-12-10 | 2000-02-18 | Disinfectant composition |
| KR1020027007411A KR100737951B1 (en) | 1999-12-10 | 2000-12-08 | Microbicide compositions |
| CN00818808A CN1433270A (en) | 1999-12-10 | 2000-12-08 | Microbicide compositions |
| PCT/JP2000/008716 WO2001041571A1 (en) | 1999-12-10 | 2000-12-08 | Microbicide compositions |
| US10/149,147 US6793846B2 (en) | 1999-12-10 | 2000-12-08 | Microbicide compositions |
| EP00980019A EP1236398A4 (en) | 1999-12-10 | 2000-12-08 | Microbicide compositions |
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| JP35221799 | 1999-12-10 | ||
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| JP2000041092A JP3607555B2 (en) | 1999-12-10 | 2000-02-18 | Disinfectant composition |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001302425A (en) * | 1999-12-10 | 2001-10-31 | Kao Corp | Sterilization method |
| JP2007106727A (en) * | 2005-10-17 | 2007-04-26 | Lion Corp | Cosmetic raw material composition and antisepsis method of cosmetic raw material composition |
| JP2018035123A (en) * | 2016-09-02 | 2018-03-08 | 三菱ケミカルフーズ株式会社 | Composition and method for sterilizing spore-forming bacteria |
-
2000
- 2000-02-18 JP JP2000041092A patent/JP3607555B2/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001302425A (en) * | 1999-12-10 | 2001-10-31 | Kao Corp | Sterilization method |
| JP2007106727A (en) * | 2005-10-17 | 2007-04-26 | Lion Corp | Cosmetic raw material composition and antisepsis method of cosmetic raw material composition |
| JP2018035123A (en) * | 2016-09-02 | 2018-03-08 | 三菱ケミカルフーズ株式会社 | Composition and method for sterilizing spore-forming bacteria |
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