JP2001159630A - Blood collection container - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a blood collection container capable of efficiently and safely performing the process from the collection of blood to pretreatment operation due to a pretreatment reagent, which must not flow toward a human body by a back flow or the like, in the container. SOLUTION: In a blood collection container 1, a container main body consisting of an outer pipe 2 and an inner pipe 3 is partitioned into a first space α and a second space β, a through-hole 3x is formed to the partition wall of the first and second spaces, and the pressure of the first space α is made lower than that of the second space β and a cock 10 is attached to the through-hole 3x by the pressure difference between the first and second spaces, and when the pressure difference becomes almost zero, the cock 10 is detached from the through-hole 3x. A pretreatment reagent 7 is housed in the second space β.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a blood collection container used for clinical examination of humans and animals, and more particularly, to a single container from blood collection to pretreatment of blood components and the like. The present invention relates to a blood collection container that enables the blood collection.

[0002]

2. Description of the Related Art In the field of clinical examination for collecting and measuring blood, a vacuum blood collection tube is used to rapidly collect blood. By the way, when measuring a blood sample,
Many use plasma or serum. In measuring blood components, in order to eliminate the influence of proteins, the measurement is often performed after removing the obtained plasma or serum from proteins.

[0003] As described above, the components measured after performing the protein removal operation include, for example, ammonia, catecholamines, lactic acid, pyruvic acid, and galactose. Is bad. Therefore, when measuring these components, it is necessary to perform a protein removal operation as soon as possible after blood collection, and to add a stabilizer.

[0004] Japanese Patent Application Laid-Open No. Hei 10-248828 discloses a pretreatment container suitable for measuring the above components. Here, the pretreatment liquid is stored in a depressurized bottomed tube, and the opening of the bottomed tube is closed by a plug that can be punctured with an injection needle or the like.

[0005] However, in the inspection requiring the above-mentioned protein removal operation, the protein removal agent used for protein removal and the above-mentioned stabilizer have an adverse effect on the human body. Therefore, once blood was collected in a blood collection container, it was necessary to put a deproteinizing solution and a stabilizing agent in another test tube, and transfer the blood to the other test tube.

That is, if a protein-removing solution is previously placed in a blood collection container, such as a vacuum blood collection tube or a syringe, which communicates with a blood vessel of the human body, the protein-removing solution may flow into the body by backflow or the like. . Therefore, Japanese Patent Laid-Open No. 10-2
It is necessary to prepare a pretreatment container different from the blood collection container as in the pretreatment container described in JP-A-48828, and transfer the collected blood sample to the pretreatment container.

[0007] Therefore, there has been a problem that the transfer operation is complicated, and the blood sample comes into contact with air during the transfer, and the components are denatured. The above problem is not only the measurement of the components that require the protein removal operation, but also the case where it is necessary to perform an extraction operation with an organic solvent such as alcohol, ether or chloroform to analyze hormones and lipids. It was similar. That is, if these organic solvents are put in the blood collection container in advance, the backflow also adversely affects the human body. Therefore, when analyzing hormones and lipids, it is necessary to prepare a pretreatment container containing the above-mentioned organic solvent as a pretreatment solution for extracting these components. Work to transfer the sample was necessary.

[0008]

SUMMARY OF THE INVENTION An object of the present invention is to provide a blood sample which can be suitably used for a test requiring a pretreatment operation with a pretreatment reagent. An object of the present invention is to provide a blood collection container which can omit a complicated sample transfer operation and does not cause danger due to backflow of a pretreatment reagent.

[0009]

According to a first aspect of the present invention, there is provided a blood collection container comprising: a first space into which a blood sample is introduced;
A container body having a second space separated from the first space by a partition wall, the container body having a through-hole formed in the partition wall, and a plug body attached to close the through-hole; The pressure of the first space is lower than the pressure of the second space, so that when the pressure difference between the first and second spaces becomes substantially equal, the plug comes off the through hole, The plug body is attached to the through hole using the pressure difference, and further includes a pretreatment reagent housed in the second space.

In a specific aspect of the blood collection container according to the first invention, the container body has an outer tube and an inner tube each having an open end, and the inner tube constitutes the partition wall. And a stopper made of an elastic material for closing the openings of the outer tube and the inner tube of the container body.

In the blood collection container according to the first invention, preferably, the container body has a plurality of partition walls such that the container body has a plurality of second spaces, and a through hole is formed in each partition wall. Each through-hole is closed by a plug attached by a pressure difference between spaces on both sides of the partition wall.

A blood collection container according to a second aspect of the present invention has a bottomed outer tube and a bottomed tube which is inserted into the outer tube and whose upper end opening is lower than the upper end opening of the outer tube. An inner tube, a plug closing an upper end opening of the outer tube and an upper end opening of the inner tube, and a pretreatment reagent disposed in a gap between the inner tube and the outer tube; A notch extending downward is formed at the upper end opening edge, and the first plug closes the inner tube.
In the state of (2), the plug is pressed into the inner tube beyond the lower end of the notch, the inner tube is closed, and the second plug is pulled out.
In this state, the outer tube is closed, but the notch is exposed to allow communication between the inner tube and the gap.

A blood collection container according to a third aspect of the present invention has a bottomed outer tube and a bottomed tube which is inserted into the outer tube and whose upper end opening is lower than the upper end opening of the outer tube. An inner pipe, a plug body comprising a plurality of elastic pieces that close an upper end opening of the outer pipe and an upper end opening of the inner pipe, and a portion to be pressed into the inner pipe is pressed against an inner wall of the outer pipe; A first pretreatment reagent disposed in a gap between the inner tube and the outer tube, wherein a notch extending downward is formed at an upper end opening edge of the inner tube, and a plug closes the inner tube. In the state (1), the notch is closed by the elastic piece of the plug, and in the second state in which the plug is rotated around the axial direction, the outer tube is closed, but the elastic piece is closed by the notch. , And communication between the inner pipe and the gap is provided.

In the second and third aspects of the present invention, the bottom of the inner tube and the bottom of the outer tube are preferably joined.
Further, the blood collection container according to the fourth invention of the present application is an outer tube having upper and lower ends opened, an inner tube inserted into the outer tube, and having upper and lower ends opened; A pretreatment reagent disposed in a gap between the inner tube and the inner tube; a plug attached to close upper end openings of the outer tube and the inner tube; and a lower end opening of the inner tube and the outer tube. The inner tube and the outer tube so as to be able to assume a first state and a second state in which the inside of the inner tube and a gap between the inner tube and the outer tube communicate with each other by an external operation. A communication operation member attached to a lower end opening of the pipe.

In a specific aspect of the blood collection container according to a fourth aspect of the present invention, the lower end opening of the inner tube is located above the lower end opening of the outer tube, and the communication operation member is provided at the lower end opening of the inner tube. An outer tube insertion portion having a relatively large diameter closing the outer tube at a lower position and an inner tube insertion portion having a relatively small diameter pressed into a lower end opening of the outer tube are provided.

In another specific aspect of the present invention, a flow path having one end of the upper surface of the inner tube insertion portion opened and the other end opened to a side surface of the inner tube insertion portion is provided. A communication hole which is formed in a portion and which communicates with an opening of a flow path on a side surface of the inner tube insertion portion when the inner tube insertion portion is rotated around an axial direction in the vicinity of a lower end opening of the inner tube. Are formed.

In the second to fourth inventions, the gap between the inner pipe and the outer pipe may not be partitioned, or the gap between the inner pipe and the outer pipe may be defined by at least one partition wall extending in the axial direction. The pretreatment reagent is arranged in each section.

[0018]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the details of the blood collection container according to the present invention will be described by describing specific examples of the present invention.

FIG. 1 is a longitudinal sectional view showing a blood collection container according to a first embodiment of the present invention. FIGS. 2 and 3 are sectional views for explaining a method of using the blood collection container. It is a longitudinal cross-sectional view.

The blood collection container 1 has a container body composed of an outer tube 2 and an inner tube 3. The outer pipe 2 and the inner pipe 3 are each formed of a substantially cylindrical container having a bottom and an open end. The upper openings of the outer tube 2 and the inner tube 3 are closed by a stopper 4 made of an elastic material such as rubber.

The outer tube 2 is made of polyethylene terephthalate,
It is made of a synthetic resin such as polyethylene, polystyrene, and polypropylene, and is preferably made of a transparent synthetic resin. The inner tube 3 is also made of a suitable synthetic resin such as polystyrene terephthalate, polyethylene, polystyrene, and polypropylene, and is preferably made of a synthetic resin having transparency.

In this embodiment, the outer tube 2 and the inner tube 3 constitute a container body, and the inner tube 3 constitutes the partition wall in the first invention. That is, inside the container body,
The inner pipe 3 is partitioned into a first space and a second space. Here, the inside of the inner tube 3 is the first space, and the outer tube 2
The gap between the inner tube 3 and the inner tube 3 is a second space. A through hole 3x is formed in the bottom of the inner tube 3. The plug 10 is attached to the through hole 3x.

The plug 10 is made of a flexible material such as synthetic rubber, natural rubber or flexible synthetic resin. The pressure in the inner tube 3 is lower than the gap between the inner tube 3 and the outer tube 2. The first and second
The plug 10 is fixed by the pressure difference in the space. Therefore, when the pressure in the inner tube 3 and the pressure in the gap between the inner tube 3 and the outer tube 2 become substantially equal, the plug 10 is displaced from the through hole 3x by eliminating the pressure difference. Have been.

On the other hand, the opening 2 of the outer tube 2 into which the inner tube 3 is inserted
A plug 4 made of natural rubber or synthetic rubber is inserted from a. The stopper 4 has, at its tip, an inner tube press-fitting portion 4a having a relatively small diameter and press-fit into the opening 3a of the inner tube 3, and a grip portion 4c having a relatively larger diameter than the inner tube press-fitting portion.

The stopper 4 is configured so that it can be pierced by a vacuum blood collection needle or the like. Preferably, a thin portion (not shown) is formed at the center of the inner tube press-fitting portion 4a, thereby facilitating puncturing with a vacuum blood collection needle or the like. That is, by puncturing the stopper 4 with a vacuum blood collection needle or the like, the first
Is configured so that a blood sample is guided to the space.

In the second space, that is, in the gap between the outer tube 2 and the inner tube 3, a pretreatment reagent 7 is disposed. The pretreatment reagent 7 is a liquid reagent contained in the outer tube 2 in this embodiment. However, the pretreatment reagent is not necessarily required to be in a liquid state, but may be a solid, or may be disposed in the second space in a state where the liquid pretreatment reagent is impregnated with filter paper or the like.

When the pretreatment reagent 7 is solid, it may be fixed to the inner peripheral wall of the outer tube 2 or the inner tube 3.
May be fixed to the outer peripheral surface of the main body. As the pretreatment reagent 7, a pretreatment reagent that is dangerous when it flows back to the human body can be appropriately used, and is not particularly limited. Examples of such a pretreatment reagent include, for example, a deproteinizing solution used for measurement of ammonia, catecholamines, lactic acid, pyruvic acid, galactose and the like. Examples of such a deproteinizing solution include acids such as aqueous solutions of sodium phosphotungstate, aqueous solutions of perchloric acid, aqueous solutions of trichloroacetic acid and sulfosalicylic acid, alkalis such as sodium hydroxide, and metal salts such as sodium sulfate, ammonium sulfate and heavy metal ions. Can be mentioned. Further, an organic solvent such as alcohol, ether or chloroform used for extracting hormones and lipids for analyzing them is also used as the pretreatment reagent.

The pressure difference between the first and second spaces is equal to the first pressure difference.
The blood sample can be quickly introduced into the space by a vacuum blood sampling method or the like, and when the blood sample is introduced, the pressure difference between the first and second spaces is selected to be substantially equal.

Next, a method of using the blood collection container 1 according to the first embodiment will be described with reference to FIGS.
First, as shown in FIG. 1A, a vacuum blood collection needle A is punctured into the stopper 4 of the blood collection container 1 to guide the blood sample into the first space, that is, into the inner tube 3. The introduction of the blood sample is performed promptly because the pressure in the first space is reduced.

When the blood sample B is filled in the inner tube 3, the pressure difference between the first space, ie, the inside of the inner tube 3, and the second space, ie, the gap between the outer tube 2 and the inner tube 3, becomes almost equal. Become equal. Therefore, the plug 10 comes off from the through hole 3x (FIG. 2).
(B)).

Thereafter, as shown in FIG. 1 (c), when the stopper 10 is removed, the blood collection needle A is removed. It is desirable that the operation up to this point is performed with the bottom of the blood collection container 1 tilted upward, as shown in FIG. Otherwise, if blood leaks from the through-hole 3x and a backflow or the like occurs, the pretreatment reagent 7 may go back to the human body side.

Next, the blood collection container 1 is set upright so that the stopper 4 is positioned upward. As a result, as shown in FIG. 3A, the blood sample X flows down from the through hole 3x to the second space side and is mixed with the pretreatment reagent 7 (FIG. 3B).

Therefore, the blood collection container 1 can be turned,
By giving vibration to the blood collection container 1, the pretreatment reagent 7 and the blood sample can be mixed, and the pretreatment operation can be performed.

As described above, the through-hole 3x is closed by the stopper 10 from the time when the blood sample is collected until the time when the vacuum blood collection needle A is removed. Therefore, even if a backflow occurs between the vacuum blood collection needle and the blood vessel of the human body, there is no possibility that the pretreatment reagent 7 flows into the blood vessel of the human body.

Therefore, the use of the blood collection container 1 of the present embodiment allows a quick and easy process from blood collection to pretreatment using the pretreatment reagent 7 without transferring a blood sample.

FIGS. 4A and 4B are cross-sectional views for explaining an example of a blood collection container according to a second embodiment of the present invention, and FIG. 5 shows an outer tube thereof. It is a perspective view.
The blood collection container 1 has an outer tube 2 with a bottom, an inner tube 3 inserted into the outer tube, a stopper 4 made of rubber or the like, and a cap 5.

The outer tube 2 is made of polyethylene terephthalate,
It is made of a transparent synthetic resin such as polyethylene, polystyrene, and polypropylene. Similarly, the inner tube 3 is also made of an appropriate transparent synthetic resin such as polyethylene terephthalate, polyethylene, polystyrene, and polypropylene.

A pretreatment reagent 7 is disposed in a gap 6 between the outer tube 2 and the inner tube 3. The pretreatment reagent 7 may be a solution,
In addition, although it is impregnated with filter paper or the like and fixed to the inner peripheral surface of the outer tube 2, it may be fixed to the outer peripheral surface of the inner tube 3 or may be arranged without being fixed between the two. Good.

FIGS. 6A and 6B are a front view and a longitudinal sectional view of the outer tube 2. Below the opening 2a of the outer tube 2, a step 2b is formed on the inner peripheral surface. Step 2
b is formed to extend in the circumferential direction of the inner peripheral surface of the outer tube 2. Further, a notch 2c is formed further downward from a part of the step 2b. The notch 2c is configured so that the outer tube portion above the step 2b and the thickness in the notch 2c are equal.

The step 2b is provided to lock a lower end of a flange portion of the inner tube 3 described later. A flange portion 2d is formed on the outer peripheral edge of the opening 2a of the outer tube 2.

FIGS. 7A and 7B are a front view and a longitudinal sectional view of the inner tube 3. The inner pipe 3 has an opening 3a in the upper part, and a thick flange 3 near the opening edge of the opening 3a.
b is formed.

When the inner tube 3 is inserted into the outer tube 2, the lower end of the flange portion 3b is locked by the above-mentioned step 2b, thereby positioning the inner tube 3 in the outer tube 2.
When inserted into the outer tube 2, the opening 3a of the inner tube 3 is at a position lower than the opening 2a of the outer tube 2 (FIG. 4A).

The inner tube 3 has a notch 3c which is open at a part of the periphery of the opening 3a and extends downward. The notch 3c penetrates the wall surface of the inner pipe 3 and forms a communication part for connecting the outer pipe 2 and the inner pipe 3 as described later.

Returning to FIG. 4, a plug 4 made of natural rubber or synthetic rubber is inserted from the opening 2a of the outer tube 2 into which the inner tube 3 has been inserted. The stopper 4 has an inner tube press-fitting portion 4a which has a relatively small diameter at the tip and is press-fitted into the opening 3a of the inner tube 3 and a relatively larger diameter than the inner tube press-fitting portion. An outer tube press-fitting portion 4b to be press-fitted and a grip portion 4c located above the opening 2a of the outer tube 2 are provided. In the center of the plug 4, a thin portion 4d is formed. The thin portion 4d is provided to facilitate puncturing with a vacuum blood collection needle or the like.

On the other hand, a cap 5 is attached to the stopper 4. The cap 5 has a substantially cylindrical shape that opens downward. The cap 5 can be made of a synthetic resin such as polyethylene, polypropylene, or polystyrene, or a metal such as aluminum. Cap 5
In order to facilitate the operation of sliding the cap 5 together with the stopper 4 on the outer peripheral side surface, a plurality of annular protrusions 5a extending in the circumferential direction are formed.

A through hole 5c is formed in the top plate 5b of the cap 5. The through-hole 5c is provided to facilitate puncturing of the stopper 4 such as a vacuum blood collection needle. 8, a stopper 5d is provided on the inner circumferential surface of the cap 5 at an intermediate height position. The stopper 5d is formed to protrude inward from the inner peripheral surface of the cap 5. Further, a plurality of stoppers 5d are dispersedly formed in the circumferential direction.

The stopper 5d is provided to lock the upper end of the outer tube 2 when the stopper 4 is attached to the outer tube 2 together with the cap 5. That is, since the stopper 5d is formed, as shown in FIG. 4A, when the stopper 4 and the cap 5 are attached, the stopper 4d locks the upper end of the outer tube 2 so that the stopper 4 Inner tube 3 and outer tube 2
It is configured such that the press-fitting operation into is stopped.

On the other hand, as shown in FIG.
Below, is formed a slide stopper 5e which also projects inward. The slide stopper 5e is provided to regulate a slide amount when the stopper 4 is slid in a direction away from the inner tube 3 and the outer tube 2 together with the cap 5 as described later.

That is, the slide operation is stopped when the upper end of the slide stopper 5e contacts the lower end of the flange 2d of the outer tube 2.

Returning to FIG. 4, a method of using the blood collection container of this embodiment will be described. In the present embodiment, in the initial state, as shown in FIG. 4A, the inner tube 3 is inserted into the outer tube 2, and the plug 4 attached to the cap 5 is connected to the inner tube 3 and the outer tube 2. Press-fit. That is, the inner pipe press-fitting portion 4a of the stopper 4 closes the opening 3a of the inner pipe 3, and the outer pipe press-fitting portion 4a is closed.
b closes the opening 2 a of the outer tube 2. In this state, the lower end of the through cutout 3c provided in the inner pipe 3 is located above the lower end of the inner pipe press-fitting portion 4a of the plug 4 in this state. Therefore, the notch 3c is closed by the inner pipe press-fitting portion 4a. Therefore, the inside of the inner tube 3 is sealed.

Preferably, the pressure in the blood collection container 1 is reduced. Next, blood is collected using a vacuum blood collection needle or the like.
When collecting blood, a vacuum blood collection needle is punctured into the thin portion 4 d of the stopper 4. When the pressure inside the blood collection container 1 is reduced, blood is guided into the inner tube 3 by the negative pressure in the blood collection container. Needless to say, the inside of the blood collection container 1 does not necessarily have to be depressurized. Even in this case, the blood flows into the inner tube 3 by puncturing the thin portion 4 d of the stopper 4 with the blood collection needle.

After collecting a predetermined amount of blood, the vacuum blood collection needle or a normal blood collection needle is removed. Thereafter, by leaving the blood collection container 1 from which the blood has been collected, the collected blood is separated into serum and blood clots. Therefore, a serum sample can be obtained. The inner tube 3 of the blood collection container 1
If an anticoagulant such as heparin is introduced into the inside of the container to prevent coagulation of the collected blood, it can be separated into plasma and solid by centrifugation. Alternatively, a serum-separating agent or a plasma-separating agent may be charged into the inner tube 3 to obtain serum or plasma. In some cases, the test may be performed using a whole blood sample without separation into serum or plasma and solids.

As described above, a blood sample consisting of plasma, serum or whole blood is obtained. Then, plug 4 into cap 5
At the same time, slide up. In this case, the cap 5
Is fixed to the stopper 4, the stopper 5 is also slid together with the cap 5 by holding the cap 5 by hand and sliding it upward. The sliding operation is completed when the upper end of the slide stopper 5e contacts the lower end of the flange 2d of the outer tube 2. By the above slide operation, the stopper 4 moves upward, and FIG.
The state shown in FIG.

In the state shown in FIG.
b still seals the opening 2a of the outer tube 2,
When the inner pipe press-fitting portion 4a moves upward, the notch 3c is formed.
Is exposed. Therefore, the inside of the inner tube 3 and the gap 6 communicate with each other by the notch 3c. Therefore, the blood sample in the inner tube 3 can be guided to the gap 6 by tilting or falling the blood collection container 1 in the state shown in FIG. 7 and contact.

Therefore, similarly to the first embodiment, the pretreatment can be performed without transferring the blood sample to another container. In the state shown in FIG. 4B, the outer tube press-fitting portion 4b still seals the opening 2a of the outer tube 2, so that the blood collection container 1 is tilted or turned over as described above. The blood inside will not leak out of the blood collection container 1.

As described above, if the blood collection container 1 is used, the steps from blood collection to blood sample separation and pretreatment can be performed safely and hygienically using only the blood collection container 1. .

FIGS. 9A and 9B are cross-sectional views of a blood collection container according to a third embodiment of the present invention. The blood collection container 11 of the third embodiment includes an outer tube 12, an inner tube 13,
It has a stopper 14 and a cap 15.

The materials constituting the outer tube 12, the inner tube 13, the stopper 14 and the cap 15 are the same as those in the second embodiment, so that the description given in the second embodiment will be referred to. Omitted.

In the blood collection container 1 of the second embodiment, the inside of the inner tube 3 and the gap 6 are communicated by sliding the stopper 4 together with the cap 5 in the direction away from the outer tube 2 and the inner tube 3. The blood collection container 1 of the third embodiment.
In 1, by rotating the stopper 14 fixed to the cap 15, the inside of the inner tube 13 and the gap 16 are communicated.

As shown in FIGS. 10A and 10B, the outer tube 12 has a substantially cylindrical shape with a bottom. A step 12b is formed below the upper end opening 12a of the outer tube 12, and the step 12b extends in the circumferential direction on the inner surface of the outer tube 12.

In addition, on the periphery of the opening 12a of the outer tube 12,
A flange portion 12c is formed on the outside. On the other hand, FIG.
As shown in FIGS. 1A to 1C, the inner tube 13 has a substantially cylindrical shape with a bottom. In the vicinity of the upper opening 13a of the inner tube 13, a plurality of flange portions 1 are provided along the outer periphery of the inner tube 13.
3b is formed. The plurality of flange portions 13b are formed such that outer surfaces thereof come into contact with the inner surface of the outer tube 12 when the inner tube 13 is inserted into the outer tube 12. That is, the plurality of flange portions 13b are provided to stabilize the posture of the inner tube 13 in a state where the inner tube 13 is inserted into the outer tube 12. The inner tube 13 has an opening 13a.
And a notch 13c extending downward is formed. The notch 13c is a penetration notch penetrating the wall surface.

As shown in FIGS. 12A and 12B, the outer surface of the cap 15 having a substantially cylindrical shape opened downward makes it easy to rotate the cap 15 around the axial direction. A plurality of ribs 15a extending in the vertical direction
Are formed. A through hole 15c is formed at the center of the top plate 15b of the cap 15.

Further, a locking portion 15d is formed on the inner peripheral surface of the cap 15 at an intermediate height position. Locking part 1
5d is protruding inward, and includes a stopper 14;
It is provided for rotating the stopper 14 together with the cap 15. As shown in FIGS. 12C and 12D, the stopper 14
Has a plurality of resilient pieces 14a, 1
4a. The elastic piece 14a extends downward from the lower surface of the outer tube press-fitting portion 14b that is pressed into the outer tube.

The length of each elastic piece 14a is
Is formed longer than the notch 13c formed at the bottom. Stopper 14
Above the outer tube press-fitting portion 14b, a gripping portion 14c is connected. Further, a thin portion 14d is formed at the center of the stopper 14.

In the blood collection container 11 of the third embodiment,
As shown in FIG. 9A, the opening 13 a of the inner tube 13 is located at a position lower than the opening 12 a of the outer tube 12. In the initial state, that is, in the first state, the stopper 14 is press-fitted into the outer pipe 12 and the inner pipe 13.
b closes the opening 12a of the outer tube 12, and the lower surface of the outer tube press-fitting portion 14b closes the opening 13a of the inner tube 13. Further, in the first state, the notch 13c is closed so that the elastic piece 14a covers the notch 13c of the inner tube 13. Therefore, the inside of the inner tube 13 and the outer tube 12 is sealed.

The collection and preparation of a blood sample are performed in the same manner as in the case of the blood collection container 1 of the second embodiment. At the time of the test, in the blood collection container 11 of the third embodiment,
The cap 15 is rotated around the axial direction together with the stopper 14.
By this rotation operation, the elastic piece 14a is moved to a position not covering the notch 13c, that is, to the second position, whereby the inside of the inner tube 13 and the gap 16 are communicated via the notch 13c.

Also in this case, the opening 12 of the outer tube 12
Since a is sealed by the stopper 14, the blood sample does not leak out of the blood collection container 11. Therefore, the second
As in the case of the blood collection container 1 of the embodiment, the blood collection, blood sample preparation and pretreatment can be performed hygienically and safely without leaking the blood sample to the outside.

FIG. 13 is a partially cutaway sectional view showing a blood sampling container according to a fourth embodiment of the present invention. In the blood collection container 21, an inner tube 23 having an open upper and lower end is inserted into an outer tube 22 having an open upper and lower end. Also, in the gap 26 between the outer pipe 22 and the inner pipe 23,
A pretreatment reagent 27 is provided. The pretreatment reagent 27 is
It may be impregnated with filter paper or the like and fixed to the inner peripheral surface of the outer tube 22, or may be fixed to the outer peripheral surface of the inner tube 23. Further, the inner tube 23 and the outer tube 22 need not be fixed.

The upper structures of the inner tube 23 and the outer tube 22 are configured so as to be able to press-fit a plug, as in the second and third embodiments. Therefore, the upper structures and plugs of the inner tube 23 and the outer tube 22 or the caps attached as necessary are omitted by referring to the description of the structure of the second and third embodiments.

The feature of the fourth embodiment lies in the structure on the lower end side of the inner tube 23 and the outer tube 22. Lower end opening 23a of inner tube 23
Is located above the lower end opening 22a of the outer tube 22.

The lower end openings 2 of the inner pipe 23 and the outer pipe 22
The communication operation member 28 is inserted so as to close 3a and 22a. The communication operation member 28 is made of an elastic material such as natural rubber or synthetic rubber. The communication operation member 28 has an inner tube insertion portion 28a which is press-fitted into the inner tube 23 at the upper end, and an outer tube insertion portion 28b having a larger diameter than the inner tube insertion portion 28a is provided below the inner tube insertion portion 28a. Is formed. The vertical dimension of the inner tube insertion part 28a is shorter than the vertical dimension of the outer pipe insertion part 28b.
The outer tube insertion portion 28b is configured to seal the lower end opening 22a of the outer tube 22. Further, an operation portion 28c larger than the diameter of the outer tube 22 is formed below the outer tube insertion portion 28b.

In the initial state, as shown in FIG. 13, the inner tube insertion portion 28a is inserted into the lower end opening 23a of the inner tube 23.
Is inserted, whereby the inside of the inner tube 23 is sealed. The outer tube insertion portion 28b is inserted into the lower end opening 22a of the outer tube 22. Moreover, the upper surface of the outer tube insertion portion 28b is pressed against the lower end of the inner tube 23. Therefore, the inside of the inner tube 23 and the gap 26 are separated.

In the blood collection container 21 of the present embodiment, up to collection of the blood sample is performed in the same manner as in the second and third embodiments. Next, at the time of inspection, the communication operation member 28 is moved downward. In this case, the outer tube insertion portion 28b
The communication operation member 28 is moved to such an extent that the communication operation member 28 is not detached from the lower end. As a result, when the inner pipe insertion portion 28a moves away from the lower end of the inner pipe 23, the inside of the inner pipe 23 and the gap 26 communicate with each other. Therefore, the blood sample flows into the gap 26 from inside the inner tube 23 and comes into contact with the pretreatment reagent 27. Even in this case,
Since the outer tube insertion portion 28b seals the outer tube 22, the blood sample does not leak to the outside.

FIGS. 14A and 14B are cross-sectional views showing a modification of the blood collection container according to the fourth embodiment. Here, the inner pipe insertion portion 28a of the communicating operation member 28, the flow passage 28a ₁ having an opening opened to the upper surface and side surfaces are formed. Further, in the vicinity of the lower end of the inner tube 23,
A through hole 23b is formed. The height position of the through-hole 23b, in a state where press-fitting the communicating operation member 28 into the inner tube 23, and is configured to be the same height as the opening opened to the side surface of the channel 28a _1.

[0075] In the initial state, and an open aperture on the side surface of the flow path 28a _1, communicating the operating member 28 so that the communicating hole 23b do not overlap the inner tube 23 is positioned. Therefore, similarly to the fourth embodiment, in the initial state, the inner tube 23
The inside and the gap 26 are separated. After blood samples were collected, in the inspection rotates the communicating operation member 28 about the axial direction, an opening is open to the side of the inner pipe insertion portion 28a of the channel 28a _1, overlapping the communicating hole 23b .
As a result, the inside of the inner tube 23 communicates with the gap 26. Thus, in the case of forming the flow path 28a ₁ and the communication hole 23b, by rotating the communicating operation member 28, and the inner tube 23 can be in communication with the gap 26.

In the blood collection container of the modified example shown in FIG. 14, by rotating the communication operation member 28,
Although the inside of the inner tube and the gap are communicated with each other, the inside of the inner tube and the gap may be communicated by operating the communication operation member so as to be pulled out. For example, FIG. 14 (a), the inner pipe insertion portion 2 of the flow channel 28a ₁ of the communicating operation member 28
The height position of the opening opened on the side surface of FIG.
In (a), as shown by the imaginary line X, it may be located above the through hole 23b. In that case, the flow path 28a
_The portion of the inner tube insertion portion 28a that is open to the side surface is the inner tube 2
3, and the gap 26 is separated from the inside of the inner tube 23 in the initial state. And in the inspection,
Pull the communicating operation member 28 downward, the portions that are open on the side surface of the inner tube insertion portion 28a of the channel 28a ₁ may be caused to overlap with the through-hole 23b. As a result, the inner pipe 23 and the gap 2
6 is communicated.

[0077] Incidentally, contrary to the above, the position of the portion that is open to the side of the inner pipe insertion portion 28a of the channel 28a _1, be configured to be positioned lower than the through-hole 23b in the initial state In such a case, the communication operation member 28 may be moved upward for the inspection.

In the second and third embodiments, the inner tubes 3 and 13 are inserted into the outer tubes 2 and 12, however, the inner tube and the outer tube may be integrally formed. That is, as shown in FIG. 15A, the inner tube and the outer tube may be formed as an integrally molded product in which the inner tube 33 and the outer tube 32 are joined at the lower ends. Further, as shown in FIG.
May be joined at the lower end with an adhesive or the like.

Further, as shown in FIG. 15C, the inside of the outer tube 42 may be separated by a partition wall 43, whereby one side of the partition wall 43 may be an inner tube and the other side may be an outer tube. Further, in the second embodiment, the gap 6 is formed between the inner tube 3 and the outer tube 2 as shown in FIG.
As shown in FIG. 6B, by forming a plurality of partition walls 6 a to 6 c between the inner pipe 3 and the outer pipe 2, the gap 6 is formed.
It may be divided into a plurality of sections 6d to 6f. In this case, by arranging different pretreatment reagents in each of the sections 6d to 6f, various types of pretreatment can be performed using one blood collection container. In this case, the partition walls 6a to 6c are configured so as to reach the lower end of the gap 6 so that the blood sample does not move back and forth.

[0080]

In the blood collection container according to the first aspect of the present invention, in the initial state, the pressure in the first space is relatively low, and the pressure difference between the first and second spaces causes the pressure in the through-hole. A plug is attached. Therefore, since the pressure in the first space is low, the blood sample can be quickly introduced into the first space. Then, when the blood sample is guided and the pressure in the first space becomes substantially equal to the pressure in the second space, the stopper comes off the through hole. Therefore, before the blood sample is guided and the pressure difference between the first and second spaces becomes substantially equal, a blood collection device such as a blood collection needle connected to a human body is separated from the first space of the blood collection container. By performing the blood collection operation by arranging the through-hole of the blood collection container so as to face upward from the part for introducing blood, the pretreatment reagent can be brought into contact with the blood until the blood collection is completed. It can be reliably prevented.

After the blood collection is completed, the plug is removed because the pressure difference between the first and second spaces is almost eliminated. Therefore, the blood sample can be moved from the through hole to the second space side, whereby the blood sample can react with the pretreatment reagent.

Therefore, only one blood collection container can be used to perform the process from blood collection to pretreatment, and the complicated transfer work of a blood sample, which was conventionally required, can be omitted.

Further, there is no possibility that the pretreatment reagent flows into the human body by backflow or the like. Therefore, according to the first aspect, it is possible to efficiently and safely perform a test on a blood sample that requires a preprocessing operation.

The blood collection container according to the second aspect of the present invention is initially in the first state, in which the inner tube and the outer tube are closed by plugs, so that blood is introduced into the inner tube. Thus, blood can be collected in the blood collection container. Then, serum or plasma can be obtained by leaving the blood in this state or by centrifugation. Then, by pulling out the stopper and setting it in the second state, communication between the inside of the inner tube and the gap is established,
A serum, plasma or whole blood sample can flow from within the inner tube into the gap between the inner and outer tubes, thereby bringing the serum, plasma or whole blood sample into contact with the pretreatment reagent. Therefore, using one blood collection container,
From blood collection to pretreatment can be performed safely and hygienically.

Further, there is no possibility that the pretreatment reagent flows into the human body due to backflow or the like. In the blood collection container according to the third invention of the present application, the inner tube and the outer tube are initially closed and in the first state, and the inner tube and the outer tube are closed by the stopper, and the notch of the inner tube is formed by the elastic piece of the stopper. Since it is closed, blood can be collected in the blood collection container by introducing blood into the inner tube. Then, after the blood sample such as serum or plasma is prepared, the stopper is rotated to be in the second state, so that the inside of the inner tube and the gap are connected. Therefore, serum,
A plasma or whole blood sample can flow from within the inner tube into the gap between the inner and outer tubes, thereby allowing the serum, plasma or whole blood sample to contact the pretreatment reagent. Therefore, like the blood collection container according to the first invention, the blood collection container according to the third invention also has 1
Using one blood collection container, the process from blood collection to pretreatment can be performed safely and hygienically.

Further, when the bottom of the inner tube and the bottom of the outer tube are joined, the operation from the collection of blood to the pretreatment can be performed more stably since the inner tube is difficult to move in the outer tube. .
In the blood collection container according to the fourth invention of the present application, a stopper is attached so as to close the upper end openings of the outer tube and the inner tube, and in the first state, the lower end openings of the inner tube and the outer tube are also provided. Since it is closed, the stopper can be punctured with a blood collection needle or the like, and the blood sample can be introduced into the inner tube as in the case of the first invention. Then, serum or plasma can be obtained by allowing the blood sample to stand or by centrifugation. Also,
By operating the communication operation member and setting the second state, the inside of the inner tube and the gap between the inner and outer tubes are communicated with each other.
As in the case of the invention of the above, the serum, plasma or whole blood sample can be brought into contact with a specific pretreatment liquid, so that the operation from blood collection to pretreatment can be performed safely using one blood collection container. And hygienically.

In the fourth invention, the lower end opening of the inner tube is
A relatively large diameter outer pipe insertion portion which is located at a position above the lower end opening of the outer tube and the communication operation member closes the outer tube at a position lower than the lower end opening of the inner tube; In the case of having an inner tube insertion portion having a relatively small diameter that is press-fitted into the lower end opening, the gap between the inner tube and the inner tube and the outer tube is formed by pulling out the communication operation member from the inner tube. Can communicate.

Further, a flow path is formed in the inner tube insertion portion, wherein one end of the upper surface of the inner tube insertion portion is open, and the other end is opened on the side surface of the inner tube insertion portion. In the vicinity of the opening, in the case where the communication hole communicating with the opening of the flow path on the side surface of the inner tube insertion portion is formed when the inner tube insertion portion is rotated around the axial direction, in the first state, The opening of the inner tube insertion section on the side surface of the flow path should not overlap the communication hole, and after collecting the blood sample, insert the inner tube so that the flow path opening and the communication hole overlap. By rotating the part around the axial direction, the serum, plasma or whole blood sample can flow into the gap between the inner tube and the outer tube, whereby the serum, plasma or whole blood sample can be mixed with the pretreatment reagent. Can be contacted.

In the blood collection container according to the second to fourth aspects of the present invention, the gap between the inner tube and the outer tube is defined by at least one partition wall extending in the axial direction. When the pretreatment reagents are arranged, it is possible to easily cope with various types of pretreatment by using a plurality of pretreatment reagents.

[Brief description of the drawings]

FIG. 1 is a longitudinal sectional view of a blood collection container according to a first embodiment of the present invention.

FIGS. 2A to 2C are cross-sectional views illustrating a method of using the blood collection container according to the first embodiment.

FIGS. 3A and 3B are longitudinal sectional views for explaining a method of using the blood collection container according to the first embodiment.

FIGS. 4A and 4B are longitudinal sectional views of a blood collection container according to a second embodiment of the present invention.

FIG. 5 is a perspective view showing an outer tube of a blood collection container according to a second embodiment of the present invention.

FIGS. 6A and 6B are a front view and a longitudinal sectional view showing an outer tube of a blood collection container according to a second embodiment.

FIGS. 7A and 7B are a front view and a longitudinal sectional view of an inner tube used for a blood collection container according to a second embodiment.

FIG. 8 is a longitudinal sectional view of a cap used in the blood collection container of the second embodiment.

FIGS. 9A and 9B are longitudinal sectional views of a blood collection container according to a third embodiment of the present invention and a state in which a notch in an inner tube is closed by an elastic piece of a stopper. Cross-sectional view.

FIGS. 10 (a) and (b) are a front view and a longitudinal sectional view showing an outer tube of a blood collection container of a third embodiment.

FIGS. 11A to 11C are a front view, a longitudinal sectional view, and a plan view of an inner tube used in a blood collection container according to a third embodiment.

FIGS. 12A and 12B are a longitudinal sectional view and a front view of a cap used for a blood collection container according to a third embodiment;
(C) and (d) are the perspective view and bottom view which show the stopper used for the blood collection container of 3rd Example.

FIG. 13 is a partially cutaway longitudinal sectional view for explaining a main part of a blood collection container according to a fourth embodiment of the present invention.

FIGS. 14A and 14B are a partially cutaway longitudinal sectional view and a perspective view showing a communication operation member for describing a modified example of the blood collection container of the fourth embodiment.

FIGS. 15A to 15C are longitudinal sectional views showing modified examples of a combination type of an inner tube and an outer tube.

FIGS. 16A and 16B are cross-sectional views for explaining a gap between an inner tube and an outer tube of the blood collection container according to the second embodiment, and FIG. FIG. 9 is a cross-sectional view for explaining a modification of FIG.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 ... Blood collection container 2 ... Outer tube 2a ... Opening 2b ... Step 2c ... Notch 2d ... Flange part 3 ... Inner tube 3a ... Opening 3b ... Flange part 3c ... Notch 3x ... Through hole 4 ... Plug 4a ... Inner tube press-fitting part 4b: outer tube press-fitting part 5: cap 6: gap 6a to 6c: partition wall 6d to 6f: section 7: pretreatment reagent 10: stopper 11: blood collection container 12: outer tube 13: inner tube 13a: opening 13b ... Flange portion 13c ... Notch 14 ... Plug 14a ... Elastic piece 14b ... Outer tube press-fitting portion 15 ... Cap 21 ... Blood collection container 22 ... Outer tube 22a ... Opening 23 ... Inner tube 23a ... Opening 23b ... Communication hole 26 ... Gap 27 ... Pretreatment reagent 28 ... Communication operation member 28a ... Inner tube insertion part 28a ₁ ... Flow path 28b ... Outer tube press-fitting part

Claims (10)

[Claims] 1. A container body having a first space into which a blood sample is guided, and a second space separated from the first space by a partition wall, wherein a through hole is formed in the partition wall. A plug attached to close the through-hole, wherein a pressure in the first space is lower than a pressure in the second space, and a pressure in the first and second spaces. The plug is attached to the through-hole using the pressure difference so that the plug comes off from the through-hole when the difference becomes substantially equal, and further includes a pretreatment reagent stored in the second space. A blood collection container, characterized in that: 2. The container body has an outer tube and an inner tube each having an open end, the inner tube constituting the partition wall, and the opening of the outer tube and the inner tube of the container body. The blood collection container according to claim 1, further comprising a plug made of a closed elastic material. 3. A plurality of partition walls are provided so that the container body has a plurality of second spaces, and a through hole is formed in each partition wall, and each through hole is provided on both sides of the partition wall. The blood collection container according to claim 1 or 2, wherein the container is closed by a stopper attached by a pressure difference in the space. 4. A bottomed outer tube, a bottomed inner tube inserted into the outer tube and having an upper end opening lower than the upper end opening of the outer tube, and an upper end opening and an inner end of the outer tube. A plug closing an upper end opening of the tube, and a pretreatment reagent disposed in a gap between the inner tube and the outer tube, wherein a notch extending downward is formed at an upper end opening edge of the inner tube. In the first state in which the plug closes the inner tube, the plug is pressed into the inner tube beyond the lower end of the notch, the inner tube is closed, and the second plug is pulled out. In the state of 2, the outer tube is closed, but the notch is exposed to allow communication between the inner tube and the gap. 5. A bottomed outer tube, a bottomed inner tube inserted into the outer tube and having an upper end opening lower than an upper end opening of the outer tube, and an upper end opening and an inner end of the outer tube. A plug body consisting of a plurality of elastic pieces that closes the upper end opening of the pipe and that is pressed into the inner pipe and that is pressed against the inner wall of the outer pipe, and is disposed in a gap between the inner pipe and the outer pipe A notch extending downward at the upper end opening edge of the inner tube, and in the first state in which the stopper closes the inner tube, the notch is formed by an elastic piece of the stopper. In the second state in which the plug is closed and the stopper is rotated around the axial direction, the outer tube is closed, but the elastic piece does not cover the notch, and the gap between the inner tube and the gap is formed. A blood collection container that is communicated. 6. The blood collection container according to claim 4, wherein a bottom portion of the inner tube and a bottom portion of the outer tube are joined. 7. An outer pipe having upper and lower ends opened, an inner pipe inserted into the outer pipe, and having upper and lower ends opened, and being disposed in a gap between the outer pipe and the inner pipe. A pretreatment reagent, a plug attached so as to close upper end openings of the outer tube and the inner tube, a first state closing lower end openings of the inner tube and the outer tube, In order to be able to take a second state in which the inside of the inner tube and the gap between the inner tube and the outer tube communicate with each other by an operation from the outside,
A blood collection container, comprising: a communication operation member attached to lower end openings of the inner tube and the outer tube. 8. The relative position where the lower end opening of the inner tube is located above the lower end opening of the outer tube, and the communication operation member closes the outer tube at a position lower than the lower end opening of the inner tube. An outer tube insertion part with a large diameter,
The blood collection container according to claim 7, further comprising an inner tube insertion portion having a relatively small diameter, which is press-fitted into a lower end opening of the outer tube. 9. A flow path in which one end of the upper surface of the inner tube insertion portion is open and the other end is open to a side surface of the inner tube insertion portion is formed in the inner tube insertion portion. In the vicinity of the lower end opening, a communication hole that communicates with an opening of a flow path on a side surface of the inner tube insertion portion when the inner tube insertion portion is rotated around the axial direction is formed. The blood collection container according to the above. 10. A gap between the inner pipe and the outer pipe is defined by at least one partition wall extending in an axial direction,
The blood collection container according to any one of claims 4 to 9, wherein a measurement reagent is arranged in each compartment.