JPH02277460A - Liquid collecting tube - Google Patents
Liquid collecting tubeInfo
- Publication number
- JPH02277460A JPH02277460A JP1319834A JP31983489A JPH02277460A JP H02277460 A JPH02277460 A JP H02277460A JP 1319834 A JP1319834 A JP 1319834A JP 31983489 A JP31983489 A JP 31983489A JP H02277460 A JPH02277460 A JP H02277460A
- Authority
- JP
- Japan
- Prior art keywords
- blood
- tube
- hydrophobic
- hydrophobic substance
- tube body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000007788 liquid Substances 0.000 title claims abstract description 38
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 63
- 210000004369 blood Anatomy 0.000 claims abstract description 47
- 239000008280 blood Substances 0.000 claims abstract description 47
- 239000000126 substance Substances 0.000 claims abstract description 37
- 208000007536 Thrombosis Diseases 0.000 claims abstract description 31
- 238000007789 sealing Methods 0.000 claims description 19
- -1 polybutylene Polymers 0.000 claims description 11
- 238000005119 centrifugation Methods 0.000 claims description 9
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 6
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 6
- 229920001451 polypropylene glycol Polymers 0.000 claims description 6
- 230000005484 gravity Effects 0.000 claims description 3
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 claims description 3
- 229920001748 polybutylene Polymers 0.000 claims description 3
- 210000002966 serum Anatomy 0.000 abstract description 13
- 210000000601 blood cell Anatomy 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 5
- 238000000926 separation method Methods 0.000 abstract description 3
- 230000007774 longterm Effects 0.000 abstract description 2
- 239000012528 membrane Substances 0.000 abstract description 2
- 238000004321 preservation Methods 0.000 abstract 1
- 239000000306 component Substances 0.000 description 28
- 239000004033 plastic Substances 0.000 description 10
- 229920003023 plastic Polymers 0.000 description 10
- 239000011521 glass Substances 0.000 description 9
- 229920001296 polysiloxane Polymers 0.000 description 9
- 238000012360 testing method Methods 0.000 description 6
- 230000023555 blood coagulation Effects 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 229920000139 polyethylene terephthalate Polymers 0.000 description 4
- 239000005020 polyethylene terephthalate Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000010241 blood sampling Methods 0.000 description 3
- 238000009534 blood test Methods 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 230000004888 barrier function Effects 0.000 description 2
- 230000015271 coagulation Effects 0.000 description 2
- 238000005345 coagulation Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 2
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 2
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 239000012503 blood component Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000010102 injection blow moulding Methods 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 229920002239 polyacrylonitrile Polymers 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 229920006268 silicone film Polymers 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/04—Investigating sedimentation of particle suspensions
- G01N15/05—Investigating sedimentation of particle suspensions in blood
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Dispersion Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
- External Artificial Organs (AREA)
Abstract
Description
【発明の詳細な説明】
［産業上の利用分野］
本発明は疎水性成分を含有する液体を採取するための採
液管であって、当該疎水性成分の管内面への付着を防止
しうる採液管に関する。[Detailed Description of the Invention] [Industrial Application Field] The present invention is a liquid collection tube for collecting a liquid containing a hydrophobic component, which can prevent the hydrophobic component from adhering to the inner surface of the tube. Regarding liquid collection tubes.
［従来の技術１
−Ｍに、採液管たとえば血液の生化学検査に用いられる
採血管としては；ガラス管あるいはプラスチック管が用
いられている。[Prior art 1-M] A glass tube or a plastic tube is used as a blood sampling tube, for example, a blood sampling tube used for biochemical testing of blood.
ところで、このような採血管では、採血後に血液成分の
遠心分離が行なわれ、血清成分と血餅成分とに分離され
るが、このうち血餅成分は疎水性であり、管内面に付着
しやすい。この血餅が管内面の特に上方部に付着すると
、上方に遠心分離された血清中に血球が混入して検査値
に影響を与えるおそれがあり、検査精度が低下する。By the way, in such blood collection tubes, after blood collection, the blood components are centrifuged and separated into serum components and blood clot components. Of these, the blood clot components are hydrophobic and tend to adhere to the inner surface of the tube. . If this blood clot adheres to the inner surface of the tube, particularly in the upper part, blood cells may be mixed into the serum centrifuged upward and affect test values, reducing test accuracy.
このため、従来、ガラス製の採血管では、血餅の付着を
防止するために、その内面に血餅付着防止剤がコーティ
ングされている。そして、ガラス管の場合には、ガラス
自体が血液凝固促進作用を持っているため、その特色を
生かすために血餅付着防止剤として、水溶性シリコーン
がコーティングされていた。すなわち、このガラス管に
おいては、血液が管内に採取されると、水溶性シリコー
ンと採取された血液が接触して水溶性シリコーンは溶解
し、その結果、清浄なガラスの表面が露出する。このた
め血液の一部はガラスの表面に接触することになるが、
清浄なガラスの表面は親水性であるため、血液の凝固が
促進され、血餅の形成が短時間に行われるとともに、管
内面に血餅が付着しないものができる。For this reason, the inner surface of glass blood collection tubes has conventionally been coated with a blood clot-preventing agent in order to prevent blood clots from adhering to the tubes. In the case of glass tubes, since the glass itself has a blood coagulation promoting effect, in order to take advantage of this characteristic, the tubes were coated with water-soluble silicone as an anti-blood clot adhesion agent. That is, in this glass tube, when blood is collected into the tube, the water-soluble silicone comes into contact with the collected blood and the water-soluble silicone dissolves, thereby exposing the clean glass surface. Because of this, some of the blood comes into contact with the glass surface,
Since the surface of clean glass is hydrophilic, blood coagulation is promoted, blood clots are formed in a short time, and blood clots do not adhere to the inner surface of the tube.
［発明が解決しようとする課題］
しかしながら、プラスチック製の採血管の場合には、プ
ラスチックは血液凝固促進作用を有しないので、管内に
別に血液凝固促進剤を入れて凝固を促進させている。こ
のため、プラスチック製の採血管では疎水性シリコーン
を使用する必要はないが、プラスチックは疎水性である
ため別途血餅の付着を防止する方法が要望されている。[Problems to be Solved by the Invention] However, in the case of plastic blood collection tubes, since plastic does not have a blood coagulation promoting effect, a blood coagulation promoter is separately placed inside the tube to promote coagulation. For this reason, it is not necessary to use hydrophobic silicone in plastic blood collection tubes, but since plastic is hydrophobic, there is a need for a separate method to prevent blood clots from adhering.
なお、プラスチック製の採血管においても、管内面を水
溶性シリコーンでコーティングして血餅の付着を防止す
ることも考えられるが、この場合には、長期間（１日〜
２日）保存した後に遠心分離すると、水溶性シリコーン
が血液中に溶けてしまうために、結局管内面に薄く血餅
が付着してしまうという問題があった。It is also possible to coat the inner surface of plastic blood collection tubes with water-soluble silicone to prevent blood clots from adhering;
2 days) When centrifuged after storage, water-soluble silicone dissolves in blood, resulting in a thin blood clot adhering to the inner surface of the tube.
本発明はかかる問題点に鑑みてなされたものであって、
プラスチック製の管体な用いた場合でも、採取した液体
中の疎水性成分が管内面に付着することがなく、したが
って遠心分離後に疎水性成分の他の成分中への混入を防
止することができ、精度良い液体検査を可能とする採液
管を提供することを目的とする。The present invention has been made in view of such problems, and includes:
Even when a plastic tube is used, hydrophobic components in the collected liquid do not adhere to the inner surface of the tube, which prevents the hydrophobic components from mixing with other components after centrifugation. The purpose of the present invention is to provide a liquid collection tube that enables highly accurate liquid testing.
［課題を解決するための手段］
上記課題を解決するために、本発明による採液管は、互
いに比重の異なる２種以上の成分からなるともに当該成
分のうち少なくとも１種以上の成分が疎水性を有する液
体を採取し、かつ該液体を遠心分離するために用いられ
る採液管であって、管内面のうち少なくとも前記遠心分
離後に疎水性成分以外の成分が接触することになる部分
に、所定の遠心力を付加したときに実質的に流動性を示
す疎水性物質を固定させたことを特徴とし、また、本発
明による採液管は、さらに封止部材を備え、該封止部材
の内面にも前記疎水性物質を固定させたことを特徴とす
るものである。[Means for Solving the Problems] In order to solve the above problems, the liquid collection tube according to the present invention is composed of two or more components having mutually different specific gravities, and at least one of the components is hydrophobic. A liquid collection tube used for collecting a liquid having The liquid collection tube according to the present invention is characterized in that a hydrophobic substance that exhibits substantial fluidity is fixed when a centrifugal force of The hydrophobic substance is also immobilized thereon.
この採液管においては、特に疎水性物質を管体の内面お
よび封止部材の内面に膜状に固定し、遠心力を５００〜
１５００Ｇの範囲に設定して、採取される液体を血液と
する場合に有効である。In this liquid collection tube, in particular, a hydrophobic substance is fixed in the form of a film on the inner surface of the tube body and the inner surface of the sealing member, and the centrifugal force is applied to the
It is effective when setting in the range of 1500G and the liquid to be collected is blood.
ここに、「実質的に流動性を示す」とは、流動すること
により付着した疎水性成分（たとえば血餅）をそぎ落と
すか、あるいは付着した疎水性成分とともに流動するこ
とにより特定の部位への疎水性成分の吸着を抑制できる
程度の流動性を示すことを意味するものである。Here, "substantially exhibits fluidity" means that it is capable of scraping off attached hydrophobic components (e.g. blood clots) by flowing, or by flowing together with attached hydrophobic components to a specific site. This means that it exhibits fluidity to the extent that it can suppress adsorption of hydrophobic components.
前記疎水性物質としては、ポリプロピレングリコール、
ポリブチレングリコールまたはポリビニルエチルエーテ
ルを用いることが好ましい。The hydrophobic substance includes polypropylene glycol,
Preference is given to using polybutylene glycol or polyvinylethyl ether.
［作 用］
上記疎水性物質は、所定の遠心力、具体的には、たとえ
ば５００〜１５００Ｇの範囲の遠心力を付加したときに
実質的に流動性を示すものであるため、採取する液体と
の接触面が流動し、−旦付着した疎水性成分はこの流動
面に沿って、あるいは流動面に吸着しながら流下するこ
とになる。[Function] The above-mentioned hydrophobic substance exhibits substantial fluidity when a predetermined centrifugal force is applied, specifically, for example, a centrifugal force in the range of 500 to 1500G, so it is compatible with the liquid to be collected. The contact surface of the fluid flows, and the hydrophobic component that has previously adhered flows down along this fluid surface or while being adsorbed to the fluid surface.
したがって上記疎水性物質を管内面の、特に遠心分離後
に疎水性成分以外の成分が接触することになる部分に固
定させた本発明の採液管にあっては、疎水性成分が付着
しようとしても、前記疎水性物質とともに流動するため
、疎水性成分が当該部分に付着することがない。Therefore, in the liquid collection tube of the present invention in which the above-mentioned hydrophobic substance is immobilized on the inner surface of the tube, especially at the part that will come into contact with components other than the hydrophobic components after centrifugation, even if the hydrophobic components try to adhere, , because it flows together with the hydrophobic substance, the hydrophobic component does not adhere to the part.
すなわち、本発明の採液管は管体がプラスチック製であ
っても、疎水性成分の管内面への付着を効果的に防止す
ることができるものである。That is, even if the tube body of the liquid collection tube of the present invention is made of plastic, it is possible to effectively prevent hydrophobic components from adhering to the inner surface of the tube.
また、上記疎水性物質を封止部材の内面に固定させた採
液管にあっても、同様に疎水性成分の封止部材の内面へ
の付着を防止することができる。Furthermore, even in the case of a liquid collection tube in which the hydrophobic substance is fixed to the inner surface of the sealing member, it is possible to similarly prevent the hydrophobic component from adhering to the inner surface of the sealing member.
さらに、本発明の採液管にあっては、疎水性物質が採取
された液体に実質的に溶けださない状態で管内面に固定
されているために、長期間保存した場合でも、当該疎水
性物質が溶は出すようなことがなく、この点からも管内
面に疎水性成分が付着するようなことがない。Furthermore, in the liquid collection tube of the present invention, since the hydrophobic substance is fixed to the inner surface of the tube in a state that does not substantially dissolve into the collected liquid, even when stored for a long period of time, the hydrophobic substance There is no possibility that hydrophobic substances will dissolve out, and from this point of view, there will be no possibility that hydrophobic components will adhere to the inner surface of the tube.
したがって、本発明の採液管に液体として特に血液を採
取する場合には、管内面若しくは封止部材の内面への血
餅の付着を効果的に防止することができ、遠心分離後に
血球が血清中へ混入するようなことがな（、このため血
液検査の精度が向上する。特に封止部材の内面にも疎水
性物質を固定させた場合には、採液管を傾けた場合でも
、血球の血清中への混入を防止することができる。Therefore, when particularly blood is collected as a liquid into the liquid collection tube of the present invention, it is possible to effectively prevent blood clots from adhering to the inner surface of the tube or the inner surface of the sealing member, and after centrifugation, blood cells are removed from serum. (This improves the accuracy of blood tests. Especially when a hydrophobic substance is also fixed on the inner surface of the sealing member, even if the sample tube is tilted, blood cells will not be mixed in.) can prevent contamination of blood into serum.
［実施例］
以下、本発明の実施例を図面を参照して具体的に説明す
る。[Example] Hereinafter, an example of the present invention will be specifically described with reference to the drawings.
第１図は本発明の一実施例に係る減圧採血管を示す縦断
面図である。図中、ｌはプラスチックにより成形され、
一端部に開口部２ａを有するとともに他端部２ｂが閉塞
された有底管体である。この有底管体ｌの内面の全面に
は、疎水性成分としての血餅の付着を防止するための疎
水性物質３が膜状に固定されている。この疎水性物質３
は、当該採血管に、たとえば５００〜１５００Ｇの範囲
の遠心力を付加したときに実質的に流動性を示し、管底
方向へ移動するものが用いられる。この疎水性物質３と
しては、具体的にはポリプロピレングリコール、ポリブ
チレングリコールおよびポリビニルエチルエーテル中の
少なくともいずれか１種が好ましい。そして、この疎水
性物質３を管体ｌの内面に膜状に形成するには、たとえ
ば当該疎水性物質３の溶液に浸漬させた後に乾燥させる
、いわゆる溶液コート法が用いられる。FIG. 1 is a longitudinal sectional view showing a reduced pressure blood collection tube according to an embodiment of the present invention. In the figure, l is molded from plastic,
It is a bottomed tube having an opening 2a at one end and a closed end 2b. A hydrophobic substance 3 for preventing the adhesion of blood clots as a hydrophobic component is fixed in the form of a film on the entire inner surface of the bottomed tube l. This hydrophobic substance 3
used is one that exhibits substantial fluidity and moves toward the bottom of the tube when a centrifugal force in the range of 500 to 1500 G is applied to the blood collection tube. Specifically, the hydrophobic substance 3 is preferably at least one of polypropylene glycol, polybutylene glycol, and polyvinylethyl ether. In order to form the hydrophobic substance 3 in the form of a film on the inner surface of the tubular body 1, a so-called solution coating method is used, for example, in which the hydrophobic substance 3 is immersed in a solution and then dried.
また、有底管体１の開口部２ａは、封止部材４により内
部に血液凝固促進剤５を封入した状態で、かつ所定の減
圧状態を維持するように封止されている。この封止部材
４は、たとえばアルミニウムにより形成されるとともに
有底管体ｌの開口部２を覆うガスバリヤ−性のフィルム
部材４ａと、このフィルム部材４ａ上で開口部２ａに対
向させて接着固定された再シールゴム部材４ｂとにより
構成され、血液の採取時にはこれらの再シールゴム部材
４ｂおよびフィルム部材４ａには、図示しない採血ホル
ダーに設けられた中空の穿刺針が穿刺され、有底管体ｌ
の内部に刺通されることとなる。すなわち、穿刺針の先
端から導入された血液は、有底管体１の内部の減圧状態
により、穿刺針の中空部を通って採血管の内部に流入し
、これにより採血が行われるものである。Further, the opening 2a of the bottomed tube 1 is sealed by a sealing member 4 so as to maintain a predetermined reduced pressure state with the blood coagulation promoter 5 sealed therein. This sealing member 4 is formed of, for example, aluminum and has a gas barrier film member 4a that covers the opening 2 of the bottomed tube l, and is adhesively fixed on the film member 4a to face the opening 2a. When collecting blood, a hollow puncture needle provided in a blood collection holder (not shown) is inserted into the resealing rubber member 4b and the film member 4a, and the bottomed tube l
It will be pierced inside. That is, blood introduced from the tip of the puncture needle flows into the blood collection tube through the hollow part of the puncture needle due to the reduced pressure inside the bottomed tube body 1, and blood is thus collected. .
上記有底管体ｌのプラスチック素材としては、ポリエチ
レン、ポリプロピレン、ポリ（４−メチルペンテン）、
ポリスチレン、ポリカーボネート、ポリメチルメタアク
リレート、ポリエチレンテレフタレート、ナイロン、ア
クリルニトリル系ポリマー等の透明性をある程度有する
ものが用いられる。なお、有底管体１の内部には、その
他抗凝固剤、血清分離剤等を封入してもよい。Plastic materials for the bottomed tube l include polyethylene, polypropylene, poly(4-methylpentene),
Materials having some degree of transparency such as polystyrene, polycarbonate, polymethyl methacrylate, polyethylene terephthalate, nylon, and acrylonitrile polymers are used. Note that other anticoagulants, serum separating agents, etc. may be sealed inside the bottomed tube 1.
上記本実施例による減圧採血管においては、有底管体ｌ
の内面全体に疎水性物質３が膜状を呈して存在するため
、採血を行った後、管体を第２図（ａ）に示すように直
立させると、管内面において血液７が接触しないことと
なる上部分では血液７が直ちにはじかれ、また血液７が
接触することとなる部分にあっても管内面に血餅が付着
する°ことはない。また、長期間保存した後に５００〜
１５００Ｇの範囲の遠心力で遠心分離を行うと、第２図
（ｂ）に示すように、血清８が上部に、一方血餅９が下
部に分離されるが、このとき管体１の内面上部に付着し
た血餅９は疎水性物質３とともに底部方向に流動する。In the reduced pressure blood collection tube according to the present embodiment, the bottomed tube body l
Since the hydrophobic substance 3 is present in a film form on the entire inner surface of the tube, if the tube body is stood upright as shown in FIG. 2(a) after blood collection, the blood 7 will not come into contact with the inner surface of the tube. The blood 7 is immediately repelled in the upper part, and even in the part where the blood 7 comes into contact, blood clots do not adhere to the inner surface of the tube. In addition, after long-term storage, 500 ~
When centrifugation is performed with a centrifugal force in the range of 1500 G, the serum 8 is separated into the upper part and the blood clot 9 is separated into the lower part, as shown in FIG. 2(b). The blood clot 9 adhering to the surface flows toward the bottom together with the hydrophobic substance 3.
すなわち、血餅９が管内面の上部に付着することがなく
、したがって血球が血清中に混入し、検査に影響を与え
ることがない。That is, the blood clot 9 will not adhere to the upper part of the inner surface of the tube, and therefore blood cells will not be mixed into the serum and affect the test.
以上に実施例を挙げて本発明を説明したが、本発明は上
記実施例に限定されるものではなく、本発明の要旨を変
更しない範囲で種々変更可能である。たとえば、上記実
施例においては、疎水性物質３を有底管体ｌの内面全体
に固定させるようにしたが、この疎水性物質３は少なく
とも有底管体ｌの内面の遠心分離後の血清８が接触する
範囲に固定されていればよい。また、遠心分離後には、
封止部材４の内面にも血餅９が付着することがあり、こ
のため採血管を傾けたときに血清８が血餅９に接触する
おそれがある。Although the present invention has been described above with reference to Examples, the present invention is not limited to the above-mentioned Examples, and can be modified in various ways without changing the gist of the present invention. For example, in the above embodiment, the hydrophobic substance 3 was fixed to the entire inner surface of the bottomed tube l, but the hydrophobic substance 3 was fixed to at least the serum 8 after centrifugation on the inner surface of the bottomed tube l. It suffices if it is fixed within the range where it contacts. In addition, after centrifugation,
Blood clots 9 may also adhere to the inner surface of sealing member 4, and therefore, there is a risk that serum 8 may come into contact with blood clots 9 when the blood collection tube is tilted.
したがって、第３図（ａ）、（ｂ）に示すように疎水性
物質３の膜を有底管体ｌの内面だけでな（封止部材４の
内面にも形成することが好ましい。すなわち、同図（ａ
）は第１図の採血管の封止部材４の内面にも疎水性物質
３の膜を形成した状態を示すものである。このような構
成とすることにより、同図（ｂ）に示すように採血管を
傾けた場合において、血餅９が封止部材４の内面に付着
しようとしても、この血餅９は遠心分離の際に疎水性物
質３とともに流下することになる。なお、この疎水性物
質３の膜は封止部材４の内面のみに形成するようにして
もよい。Therefore, as shown in FIGS. 3(a) and 3(b), it is preferable to form the film of the hydrophobic substance 3 not only on the inner surface of the bottomed tube l (also on the inner surface of the sealing member 4). The same figure (a
) shows a state in which a film of the hydrophobic substance 3 is also formed on the inner surface of the sealing member 4 of the blood collection tube shown in FIG. With this configuration, even if the blood clot 9 tries to adhere to the inner surface of the sealing member 4 when the blood collection tube is tilted as shown in FIG. In this case, it flows down together with the hydrophobic substance 3. Note that the film of the hydrophobic substance 3 may be formed only on the inner surface of the sealing member 4.
さらに、上記実施例においては、疎水性物質３をコーテ
ィングすることにより有底管体ｌの内面に膜を形成させ
るようにしたが、これはたとえばこの疎水性物質３を有
底管体１の素材物質に予め混合させておいてから、通常
の射出成形法やブロー成形法により管体を成形すること
により、疎水性物質３を有底管体１の表面に分散させる
ようにしてもよい。この場合には、若干透明性が損なわ
れるものの一上記効果は得られるものである。さらには
、この方法と上記コーティング法とをイ井用してもよい
。Furthermore, in the above embodiment, a film was formed on the inner surface of the bottomed tube 1 by coating the hydrophobic substance 3. The hydrophobic substance 3 may be dispersed on the surface of the bottomed tube 1 by mixing it with a substance in advance and then molding the tube by a normal injection molding method or blow molding method. In this case, the above-mentioned effect can be obtained although transparency is slightly impaired. Furthermore, this method and the coating method described above may also be used.
また、上記実施例においては、採取される液体を血液と
して、遠心分離後の血球の血清部分への混入を防止する
例について説明したが、一般に疎水性成分を含む互いに
比重の異なる２種以上の成分からなる液体の分離後の疎
水性成分の他の成分中への混入を防止するような場合に
も適用が可能である。In addition, in the above embodiment, an example was explained in which blood is used as the collected liquid to prevent blood cells from being mixed into the serum portion after centrifugation. However, in general, two or more types of liquid containing hydrophobic components and having different specific gravities are used. It can also be applied to cases where a hydrophobic component is prevented from being mixed into other components after separation of a liquid consisting of the components.
次に、本発明者は上記本発明の効果を確認するために以
下のような実験を行った。Next, the inventor conducted the following experiment to confirm the effects of the present invention.
（実験例）
ポリエチレンテレフタレート樹脂（三井ペット（株）製
、商品名ＪＯ２５：ＢＯ１Ｇ＝７：３ ）により作られ
た採血管を、ポリプロピレングリコール０．２％のエタ
ノール溶液に浸漬させた後、乾燥させることにより、内
面全体にポリプロピレングリコールの膜を固定させた。(Experiment example) A blood collection tube made of polyethylene terephthalate resin (manufactured by Mitsui Pet Co., Ltd., trade name JO25:BO1G=7:3) is immersed in an ethanol solution of 0.2% polypropylene glycol, and then dried. As a result, a polypropylene glycol film was fixed to the entire inner surface.
そして、管内に凝固促進フィルム（ポリエチレンテレフ
タレートフィルムに、ガラス粉末：ポリビニルピロリド
ン（ＰＶＰ）＝３＝１をコーティングしたもの）を入れ
た後、ガスバリヤ−性の封止部材で封止した。Then, a coagulation accelerating film (a polyethylene terephthalate film coated with glass powder: polyvinylpyrrolidone (PVP)=3=1) was placed inside the tube, and then the tube was sealed with a gas barrier sealing member.
上記減圧採血管を用いて、採血実験として５人から５本
づつ採血を行った。採血した後、採血管を４℃の温度で
２４時間保存した後に、１２００Ｇで１０分間遠心分離
を行い、管内面の血餅の付着状況について観察した。Using the vacuum blood collection tubes described above, five blood samples were collected from each of five people in a blood sampling experiment. After blood collection, the blood collection tube was stored at a temperature of 4° C. for 24 hours, centrifuged at 1200 G for 10 minutes, and the state of adhesion of blood clots on the inner surface of the tube was observed.
同時に、水溶性シリコーン（トーレシリコーン（株）製
、商品名５Ｈ３７７１） ０．２％のエタノール溶液に
浸漬させた後、乾燥させて水溶性シリコーン膜を固定さ
せたポリエチレンテレフタレート管についても採血して
比較を行った。At the same time, blood was collected from polyethylene terephthalate tubes that had been immersed in a 0.2% ethanol solution of water-soluble silicone (manufactured by Toray Silicone Co., Ltd., trade name 5H3771) and then dried to fix the water-soluble silicone membrane. I did it.
その結果、ポリプロピレングリコールの膜を固定させた
管は、水溶性シリコーンの膜を形成したものよりも、管
内面への血餅付着量が少なく良好な結果が得られた。As a result, the tubes fixed with the polypropylene glycol film showed better results with less blood clots adhering to the inner surface of the tube than the tubes with the water-soluble silicone film.
［発明の効果］
以上説明したように本発明による採液管においては、疎
水性物質は、所定の遠心力を付加したときに実質的に流
動性を示すものであるため、採取する液体との接触面が
流動し、−旦付着した疎水性成分はこの流動面に沿って
、あるいは流動面に吸着しながら流下することになり、
その結果、当該部分に疎水性成分が付着するおそれがな
（なる。特に液体が血液である場合には、血餅の管内面
や封止部材の内面への付着を防止できるとともに、血球
の血清部分中への混入を防止でき、血液検査に影響を及
ぼすことがなく、検査精度が向上するという効果を奏す
る。[Effects of the Invention] As explained above, in the liquid collection tube according to the present invention, the hydrophobic substance substantially exhibits fluidity when a predetermined centrifugal force is applied to it, so that it does not interact with the liquid to be collected. The contact surface flows, and the hydrophobic components that have adhered to it flow down along this flow surface or while being adsorbed to the flow surface.
As a result, there is no risk of hydrophobic components adhering to the area.Especially when the liquid is blood, it is possible to prevent blood clots from adhering to the inner surface of the tube or sealing member, and to prevent the adhesion of blood clots to the inner surface of the tube or sealing member. It is possible to prevent contamination into the blood test area, have no effect on blood tests, and improve test accuracy.
【図面の簡単な説明】
第１図は本発明の一実施例に係る減圧採血管の縦断面図
、第２図（ａ）、（ｂ）はそれぞれ上記採血管の遠心分
離の前後の状態を説明するための縦断面図、第３図（ａ
）、（ｂ）はそれぞれ本発明の他の実施例に係る減圧採
血管を示す縦断面図である。
■・・・有底管体、
３・・・疎水性物質、
７・・・血液、
９・・・血餅
２ａ・・・開口部
４・・・封止部材
８・・・血清
（Ｑ）
第３図[BRIEF DESCRIPTION OF THE DRAWINGS] FIG. 1 is a longitudinal sectional view of a vacuum blood collection tube according to an embodiment of the present invention, and FIGS. 2(a) and (b) show the states of the blood collection tube before and after centrifugation, respectively. A vertical cross-sectional view for explanation, FIG. 3 (a
) and (b) are longitudinal cross-sectional views showing reduced pressure blood collection tubes according to other embodiments of the present invention, respectively. ■...Bottomed tube, 3...Hydrophobic substance, 7...Blood, 9...Blood clot 2a...Opening 4...Sealing member 8...Serum (Q) Figure 3
Claims (6)
に当該成分のうち少なくとも１種以上の成分が疎水性を
有する液体を採取し、かつ該液体を遠心分離するために
用いられる採液管であって、当該管体の内面のうち少な
くとも前記遠心分離後に疎水性成分以外の成分が接触す
ることになる部分に、所定の遠心力を付加したときに実
質的に流動性を示す疎水性物質を固定させたことを特徴
とする採液管。(1) A liquid collection tube used to collect a liquid consisting of two or more components with different specific gravity, at least one of which is hydrophobic, and to centrifuge the liquid. and a hydrophobic substance that exhibits substantial fluidity when a predetermined centrifugal force is applied to at least a portion of the inner surface of the tube that will come into contact with components other than the hydrophobic component after the centrifugation. A liquid collection tube characterized by being fixed.
材の内面にも前記疎水性物質を固定させてなる請求項１
記載の採液管。(2) Claim 1, wherein the liquid collection tube further includes a sealing member, and the hydrophobic substance is also fixed to the inner surface of the sealing member.
Sample tube as described.
面に膜状に固定させてなる請求項２に記載の採液管。(3) The liquid collection tube according to claim 2, wherein the hydrophobic substance is fixed in the form of a film on the inner surface of the tube body and the inner surface of the sealing member.
てなる請求項１ないし３のいずれか１に記載の採液管。(4) The liquid collection tube according to any one of claims 1 to 3, wherein the centrifugal force is set in a range of 500 to 1500G.
ポリブチレングリコールおよびポリビニルエチルエーテ
ルのうちの少なくとも一種類である請求項１ないし４の
いずれか１に記載の採液管。(5) The hydrophobic substance is polypropylene glycol,
The liquid collection tube according to any one of claims 1 to 4, which is at least one of polybutylene glycol and polyvinylethyl ether.
血液中の血餅である請求項５に記載の採液管。(6) The liquid collection tube according to claim 5, wherein the liquid to be collected is blood, and the hydrophobic component is a blood clot in the blood.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1319834A JP2819325B2 (en) | 1988-12-16 | 1989-12-08 | Sample tube |
| FR8916574A FR2640753B1 (en) | 1988-12-16 | 1989-12-14 | TUBE FOR COLLECTING A LIQUID CONTAINING AT LEAST ONE HYDROPHOBIC COMPONENT WHICH AVOID ADHESION TO THE INTERNAL WALL OF THE TUBE |
| IT02270389A IT1237910B (en) | 1988-12-16 | 1989-12-15 | LIQUID COLLECTION TUBE |
| BE8901339A BE1004836A3 (en) | 1988-12-16 | 1989-12-15 | Tube collector fluid. |
| AU46898/89A AU618435B2 (en) | 1988-12-16 | 1989-12-15 | Liquid collecting tube |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63-317756 | 1988-12-16 | ||
| JP31775688 | 1988-12-16 | ||
| JP1319834A JP2819325B2 (en) | 1988-12-16 | 1989-12-08 | Sample tube |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH02277460A true JPH02277460A (en) | 1990-11-14 |
| JP2819325B2 JP2819325B2 (en) | 1998-10-30 |
Family
ID=26569135
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1319834A Expired - Fee Related JP2819325B2 (en) | 1988-12-16 | 1989-12-08 | Sample tube |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JP2819325B2 (en) |
| AU (1) | AU618435B2 (en) |
| BE (1) | BE1004836A3 (en) |
| FR (1) | FR2640753B1 (en) |
| IT (1) | IT1237910B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040059255A1 (en) * | 2002-09-23 | 2004-03-25 | Dimitrios Manoussakis | High bias gel tube and process for making tube |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3920549A (en) * | 1974-03-18 | 1975-11-18 | Corning Glass Works | Method and apparatus for multiphase fluid collection and separation |
| US4257886A (en) * | 1979-01-18 | 1981-03-24 | Becton, Dickinson And Company | Apparatus for the separation of blood components |
| US4308232A (en) * | 1979-07-09 | 1981-12-29 | Sherwood Medical Industries Inc. | Anticoagulant stopper coating |
| AU559940B2 (en) * | 1979-09-11 | 1987-03-26 | Terumo Corp. | Blood collecting tube |
| US4310430A (en) * | 1979-09-11 | 1982-01-12 | Terumo Corporation | α-Olefin-dialkylmaleate-based liquid separating agent |
| JPS59221666A (en) * | 1983-05-31 | 1984-12-13 | Terumo Corp | Blood separating tube |
| FR2555074B3 (en) * | 1983-11-18 | 1986-07-18 | Ohayon Hanania | DEVICE FOR SEPARATING BLOOD AND OBTAINING SERUM |
-
1989
- 1989-12-08 JP JP1319834A patent/JP2819325B2/en not_active Expired - Fee Related
- 1989-12-14 FR FR8916574A patent/FR2640753B1/en not_active Expired - Lifetime
- 1989-12-15 IT IT02270389A patent/IT1237910B/en active IP Right Grant
- 1989-12-15 BE BE8901339A patent/BE1004836A3/en not_active IP Right Cessation
- 1989-12-15 AU AU46898/89A patent/AU618435B2/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| FR2640753B1 (en) | 1993-07-30 |
| BE1004836A3 (en) | 1993-02-09 |
| AU618435B2 (en) | 1991-12-19 |
| IT8922703A0 (en) | 1989-12-15 |
| IT1237910B (en) | 1993-06-18 |
| JP2819325B2 (en) | 1998-10-30 |
| AU4689889A (en) | 1990-07-19 |
| FR2640753A1 (en) | 1990-06-22 |
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