JP2581542Y2 - Blood separation tube - Google Patents

Description

[Detailed description of the invention]

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a blood separation tube.

[0002]

2. Description of the Related Art Conventionally, a blood separation tube for a clinical test is used to separate a serum or plasma component from a blood clot or a blood cell component by inserting a separating agent into the blood separation tube and using a centrifuge. The above components were separated. However, the blood separation tube has a circular cross-sectional shape, and the weight is evenly applied during centrifugation, so that there is a problem in that the separation agent causes inversion failure.

[0003] The reversal failure here means that when a blood sample is put into a blood collection tube containing a separating agent at the bottom and then a separating operation is performed by a centrifuge, sufficient movement of the separating agent occurs. Therefore, a separating agent layer is not formed between the serum or plasma layer and the clot or blood cell layer, and the serum or plasma layer and the clot or blood cell layer are not completely separated by the separating agent layer.

[0004] As a countermeasure, a bottomed blood collection tube main body having one end opened and the other end closed, a stopper for sealing the opening of the blood collection tube main body, and a blood collection tube main body that is housed and separated. A separating agent having an intermediate specific gravity between a serum or plasma component and a clot or blood cell component, and a surface other than a plane adhered to the surface of the separating agent and having the surface of the separating agent orthogonal to the central axis of the blood collection tube main body; And a test reagent held by the fixative, which causes the fixative to elute into the blood and dissolve the test drug into the blood under blood contact. A blood separation tube for separating blood components from the blood cells to the blood clot has been proposed (JP-A-60-5384).

In the blood separation tube, the surface of the separation agent is fixed in an inclined plane with respect to the central axis of the blood collection tube main body by a fixing agent. Under the action of the centrifugal force acting as the direction, it is possible to replace with serum or blood cell components by rapid flow in the tube axis direction.

However, in the above blood separation tube, even if the surface of the separating agent is fixed to a shape other than a plane perpendicular to the central axis of the blood collection tube main body, the surface fluctuates due to the action of heat, vibration and the like. There is a problem in that the separation agent easily fluctuates in the transportation stage or in the use stage, so that the separating agent quickly flows in the tube axis direction to replace serum or blood cells.

[0007] Further, a tubular body which is closed at one end and open at the other end, and a number of spherical particles which are filled and held at the closed end of the tubular body and are hydrophobic and inert to blood, or A blood separation tube comprising a barrier made of a viscous fluid in such an amount as to fill the gaps between the spherical particles and a self-closing stopper sealing the open end is disclosed (Japanese Patent Application Laid-Open No. 53-25438). ).

In this blood separation tube, the operation of dispensing the barrier is easy, there is no danger that the degree of vacuum in the blood collection tube is reduced due to residual air bubbles in the barrier, blood coagulation is promoted, and the barrier during centrifugation is further improved. Ascent is performed quickly, is inert to blood, forms a non-flowable stable separation layer after centrifugation, and therefore transports or preserves blood or blood plasma-separated blood collection tubes as they are To provide a blood separation blood collection tube suitable for

[0009] However, the spherical particles used in the blood separation tube are not suitable for analysis such as quantification of drugs in blood.
There is a problem that the spherical particles must be controlled in the manufacturing process in order to have a possibility of affecting the drug such as adsorption, and to quickly perform the raising operation of the barrier during centrifugation. there were.

[0010]

SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned drawbacks, and has as its object to enable a quick and reliable centrifugation operation without causing inversion inversion of a separating agent and preventing serum separation. Another object of the present invention is to provide a blood separation tube that can obtain a correct test result because it can be collected in a state where there is no risk of contamination of a blood plasma component with a blood clot or a blood cell component.

[0011]

[Means for Solving the Problems]

The blood separation tube of the present invention comprises a bottomed blood collection tube main body, a stopper, and a separating agent contained in the blood collection tube main body.

As shown in FIG. 1, the blood collection tube main body has a shape in which one end is opened and the other end is closed, as shown in FIG. A separating agent 13 is provided on the bottom of the blood collection tube body 11.
Is accommodated, and the opening 14 is sealed by the plug 12.

The cross section of the blood collection tube main body 11 perpendicular to the tube axis has an oval shape as shown in FIG. Blood collection tube 1
The cross-sectional shape of 1 does not need to be oval in the entire tube axis direction, and it is sufficient if at least a portion near the bottom where the separating agent 13 is stored is oval. Further, as shown in FIG. 3, the blood collection tube body 11 may be provided with a taper whose diameter is increased from the bottom 15 toward the opening 14.

The material used for the blood collection tube body 11 is as follows:
There is no particular limitation as long as it is transparent and used for medical devices. For example, glass; synthetic resins such as polypropylene and polyethylene terephthalate are preferably used.

The stopper 12 seals the opening 14 and can maintain a negative pressure state in the blood collection tube main body 11 corresponding to a predetermined blood collection amount, and is inert to blood. There is no particular limitation, and examples of such a material include butyl rubber and styrene-based elastomer.

The separating agent 13 has an intermediate specific gravity between the serum or plasma component to be separated and the blood clot or blood cell component, and is stored in the bottom 15 of the blood collection tube main body 11. Further, it is preferable that the separating agent has hydrophobicity and thixotropic property (thixotropic property), and is substantially inert to blood.

When blood is put into the blood separation tube of the present invention and then separated by a centrifugal separator, the separating agent 13 exhibits fluidity and is placed between the serum or plasma component and the clot or blood cell component. By moving and separating for a certain period of time, a non-flowable stable separation layer is formed. Since the serum or plasma component and the blood clot or blood cell component are separated by this separation layer, the serum or plasma component can be easily taken out and subjected to a blood test.

[0019]

The blood separation tube of the present invention has an oval cross section perpendicular to the tube axis at least near the bottom of the blood separation tube, so that it is accommodated at the bottom of the blood separation tube during centrifugation. Since uneven gravity is applied to the separated separating agent, the separating agent can be easily moved, thereby preventing inversion failure. On the other hand, if the blood separation tube has a circular cross-sectional shape,
In the centrifugal separation operation, gravity is evenly applied to the separating agent accommodated in the bottom of the blood separation tube, so that movement of the separating agent is suppressed, which causes inversion failure.

[0020]

Example 1 Example 1 A blood sample was put into a blood separation tube having an oval cross section perpendicular to the tube axis shown in FIG. 1, set in a swing rotor type centrifuge, and set to 500 G. When centrifugation was performed under the conditions described above, three out of 100 blood specimens showed defective reversal.

(Comparative Example 1) Using a blood separation tube having a circular cross section, centrifugation was performed under the same conditions as in Example 1. As a result, 21 out of 100 blood specimens showed defective reversal. Was done.

Example 2 A refrigerated blood sample was placed in the blood separation tube shown in FIG. 1, set in a swing rotor type centrifuge, and refrigerated at 1000
When centrifugation was performed under the conditions of G, none of the 100 blood specimens that caused inversion inversion was not observed.

(Comparative Example 2) When centrifugation was carried out under the same conditions as in Example 2 using a blood separation tube having a circular cross section, four out of 100 blood samples were found to have poor inversion. Was done.

[0024]

The configuration of the blood separation tube of the present invention is as described above, and the separation of the separating agent does not occur, and there is no danger of mixing serum or plasma components with blood clots or blood cell components. As a result, accurate blood test results can be provided.

[Brief description of the drawings]

FIG. 1 is a cross-sectional view of a blood separation tube according to the present invention in a tube axis direction.

FIG. 2 is a cross-sectional view of the blood separation tube of the present invention in a direction perpendicular to the tube axis direction.

FIG. 3 is a cross-sectional view in the axial direction of another blood separation tube according to the present invention.

[Explanation of symbols]

 Reference Signs List 10 blood separation tube 11 blood collection tube main body 12 plug 13 separating agent 14 opening 15 bottom

Claims (1)

(57) [Scope of request for utility model registration] 1. A bottomed blood collection tube main body, one end of which is open and the other end of which is closed, a stopper for sealing the opening of the blood collection tube main body, and a serum or plasma to be separated and housed in the blood collection tube main body. A blood separation tube comprising a separating agent having an intermediate specific gravity between a component and a blood clot or blood cell component, wherein a cross-sectional shape orthogonal to a tube axis near a bottom portion of the blood separating tube that contains at least the separating agent has an oval shape. A blood separation tube characterized by being made.