JP2001158801A - Complex mucopolysaccharide and method for extraction thereof - Google Patents
Complex mucopolysaccharide and method for extraction thereofInfo
- Publication number
- JP2001158801A JP2001158801A JP34344399A JP34344399A JP2001158801A JP 2001158801 A JP2001158801 A JP 2001158801A JP 34344399 A JP34344399 A JP 34344399A JP 34344399 A JP34344399 A JP 34344399A JP 2001158801 A JP2001158801 A JP 2001158801A
- Authority
- JP
- Japan
- Prior art keywords
- hyaluronic acid
- skin
- complex
- mucopolysaccharide
- extraction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、豚皮より抽出した
複合ムコ多糖に関する発明である。TECHNICAL FIELD The present invention relates to a complex mucopolysaccharide extracted from pig skin.
【０００２】[0002]
【従来の技術】ムコ多糖とは、糖の水酸基がアミノ酸で
置換された構造をもつアミノ糖を有する多糖の総称であ
るが、典型的なムコ多糖の一種としてヒアルロン酸（hy
aluronicacid）が存在する。このヒアルロン酸は、Ｎ−
アセチルグルコサミンとグルクロン酸とが相互に鎖状に
結合した分子量２０万から４０万の高分子化合物であ
り、非常に多量の水と結合してゲル状を呈し、関節の潤
滑作用や皮膚の柔軟性などに関与し、また、その高度な
粘り気によって細菌の浸透を防ぐことにも役立ってい
る。2. Description of the Related Art Mucopolysaccharide is a general term for a polysaccharide having an aminosugar having a structure in which the hydroxyl group of the saccharide is substituted with an amino acid. One of typical mucopolysaccharides is hyaluronic acid (hyaluronic acid).
aluronicacid). This hyaluronic acid is N-
Acetyl glucosamine and glucuronic acid are high molecular compounds with a molecular weight of 200,000 to 400,000, which are linked to each other in a chain form. It also contributes to preventing bacterial penetration by its high degree of stickiness.
【０００３】かかる機能を果たしているヒアルロン酸が
体内で減少すると、皮膚が弾力性を失いシワができるな
どの美容上のトラブルが生じるだけでなく、健康上の弊
害が生じるこになる。そこで、かかる弊害を防止するた
め、ヒアルロン酸を含有する各種の化粧品や健康食品が
提案されている。[0003] When the amount of hyaluronic acid that fulfills such a function is reduced in the body, not only a cosmetic trouble such as loss of elasticity of the skin and wrinkles occurs, but also a bad effect on health. In order to prevent such adverse effects, various cosmetics and health foods containing hyaluronic acid have been proposed.
【０００４】[0004]
【発明が解決しようとする課題】しかしながら、このよ
うなヒアルロン酸は、従来、専ら鶏のトサカなどから抽
出されており、それ以外の効率のよい抽出方法は知られ
ていなかった。However, such hyaluronic acid has heretofore been exclusively extracted from chicken squirrels and the like, and no other efficient extraction method has been known.
【０００５】本発明はかかる実情に鑑みてなされたもの
であって、ヒアルロン酸を含有する複合ムコ多糖を効率
よく抽出することを課題とする。[0005] The present invention has been made in view of such circumstances, and has as its object to efficiently extract a complex mucopolysaccharide containing hyaluronic acid.
【０００６】[0006]
【課題を解決するための手段】上記の課題を解決するた
め、鋭意研究した結果、本発明者は、豚皮の表皮層や真
皮層に存在するヒアルロン酸に着目して本発明を完成さ
せた。Means for Solving the Problems As a result of intensive studies to solve the above-mentioned problems, the present inventors have completed the present invention by focusing on hyaluronic acid present in the epidermis and dermis of pigskin. .
【０００７】すなわち、本発明は、子豚の豚皮の表皮層
や真皮層から抽出したヒアルロン酸を含有する複合ムコ
多糖である。また、本発明は、子豚の豚皮をミンチする
工程、アルカリ液に浸漬する工程、蛋白質分解酵素によ
り蛋白質を分解する工程、脱臭脱色した後、遠心分離器
などにより液体部分を抽出する工程を備えたヒアルロン
酸の抽出方法である。[0007] That is, the present invention is a complex mucopolysaccharide containing hyaluronic acid extracted from the epidermal layer or dermis layer of pigskin of piglets. The present invention also includes a step of mincing pig pig skin, a step of immersing the pig skin in an alkaline solution, a step of decomposing proteins with a protease, a step of deodorizing and decolorizing, and a step of extracting a liquid portion with a centrifuge. This is a method for extracting hyaluronic acid provided.
【０００８】[0008]
【発明の実施の形態】以下、実施例に基づいて、この発
明を更に詳細に説明する。 ［実施例１］ １．３ヶ月程度の子豚の新鮮な表皮を用意し、体毛を抜
くと共に表皮を洗浄する。 ２．次に直径０．５〜０．８ｍｍ程度のミンチ状にす
る。 ３．これを４倍程度の量のＮａＯＨ液（濃度１％）に浸
漬して１２時間程度放置する。 ４．その後、塩酸によってｐＨ値を８．６程度に戻す。 ５．このような液体に対して蛋白質分解酵素（Typsin）
を添加して３７℃、１２時間程度をかけて蛋白質を分解
させる。なお、使用する酵素の量は、液体１Ｋｇに対し
て１ｇ程度である。DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in more detail based on embodiments. [Example 1] Fresh skin of a piglet of about 1.3 months is prepared, the hair is removed, and the skin is washed. 2. Next, it is minced with a diameter of about 0.5 to 0.8 mm. 3. This is immersed in about 4 times the amount of NaOH solution (concentration 1%) and left for about 12 hours. 4. Thereafter, the pH value is returned to about 8.6 with hydrochloric acid. 5. Proteolytic enzymes (Typsin) for such liquids
To decompose the protein at 37 ° C. for about 12 hours. The amount of the enzyme used is about 1 g per 1 kg of the liquid.
【０００９】６．分流器を用いて脱脂して、原料中の脂
肪を０．５％以下する。 ７．脱脂した液体と固形物の混合物に、活性炭を１％程
度加えて２時間程度攪拌することによって脱臭、脱色さ
せる。 ８．６０００回／分の遠心分離機を使用して上清液のみ
を抽出し、沈殿物を捨てる。 ９．６００ｍｍＨｇｈの圧力下、真空乾燥して滅菌す
る。この処理により、白い固体粒状のものが得られる。 １０．この固体物を１００メッシュ以上の粉末にすれ
ば、ヒアルロン酸及びデルマタン硫酸を含有する複合ム
コ多糖が得られる。この複合ムコ多糖粉末は、常温水に
も完全に溶解するという優れた特性を有する。6. The fat in the raw material is reduced to 0.5% or less by degreasing using a flow separator. 7. Activated carbon is added to the mixture of the defatted liquid and the solid at about 1%, and the mixture is stirred for about 2 hours to deodorize and decolorize. 8. Extract only the supernatant using a centrifuge at 6000 rpm and discard the precipitate. 9. Sterilize by vacuum drying under a pressure of 600 mmHgh. By this treatment, white solid granules are obtained. 10. If this solid is made into a powder of 100 mesh or more, a complex mucopolysaccharide containing hyaluronic acid and dermatan sulfate can be obtained. This complex mucopolysaccharide powder has an excellent property that it is completely dissolved in room temperature water.
【００１０】［実施例２］ １．３ヶ月程度の子豚の新鮮な表皮を用意し、体毛を抜
くと共に表皮を洗浄する。 ２．次に直径０．５〜０．８ｍｍ程度のミンチ状にす
る。 ３．常温下、食用アルコールなどを連続的に流通させて
脱脂する。原料中の脂肪は０．５％以下にする ４．脱脂処理後、水を流通させてアルコールを除去す
る。[Example 2] Fresh skin of a piglet of about 1.3 months is prepared, the body hair is removed, and the skin is washed. 2. Next, it is minced with a diameter of about 0.5 to 0.8 mm. 3. Under normal temperature, edible alcohol and the like are continuously circulated to degrease. 3. Fat content should be less than 0.5%. After the degreasing treatment, the alcohol is removed by flowing water.
【００１１】５．真空乾燥機を用いて６０℃程度で原料
を乾燥させた後、８０メッシュ程度の粉末にする。 ６．この粉末を５倍（重量）の蒸留水と共に反応釜に入
れて、良くかき混ぜた上で常温下、ｐＨを７〜８に戻
す。 ７．蛋白質分解酵素を入れて攪拌しながら、２時間程度
かけて酵素分解する。 ８．４０メッシュのスクリーンで濾過し、濾液の５％
（重量）程度の活性炭を加えて、脱臭脱色する。そし
て、濾紙で濾過して活性炭を取り除く。 ９．濾液に少しずつ食用アルコールを入れ、白い沈殿物
が出てから４〜８時間放置し、その後、高分子蛋白の白
い沈殿物を取り除く。 １０．液体中の食用アルコールを減圧圧縮缶で回収す
る。 １１．残存する液体を真空乾燥機で乾燥させて、１００
メッシュの粉末にする。 これは、ヒアルロン酸とデルマタン硫酸を含む複合ムコ
多糖粉末である。5. After the raw material is dried at about 60 ° C. using a vacuum dryer, it is turned into a powder of about 80 mesh. 6. This powder is put into a reaction vessel together with 5 times (weight) of distilled water, mixed well, and the pH is returned to 7 to 8 at room temperature. 7. The proteolytic enzyme is added and stirred for about 2 hours to perform enzymatic decomposition. Filter through a 8.40 mesh screen, 5% of the filtrate
Activated carbon of about (weight) is added to deodorize and decolorize. Then, the activated carbon is removed by filtration through filter paper. 9. Add edible alcohol little by little to the filtrate, leave it for 4 to 8 hours after a white precipitate appears, and then remove the white precipitate of the high molecular protein. 10. The edible alcohol in the liquid is recovered in a vacuum can. 11. The remaining liquid is dried with a vacuum drier,
Make mesh powder. This is a complex mucopolysaccharide powder containing hyaluronic acid and dermatan sulfate.
【００１２】[0012]
【発明の効果】以上説明したように、本発明によれば、
ヒアルロン酸を含有する複合ムコ多糖を効率よく抽出す
ることができる。As described above, according to the present invention,
The complex mucopolysaccharide containing hyaluronic acid can be efficiently extracted.
Claims (2)
るようにしたヒアルロン酸を含有する複合ムコ多糖。1. A complex mucopolysaccharide containing hyaluronic acid which is extracted from the epidermis or dermis of pigskin of piglets.
液に浸漬する工程、蛋白質分解酵素により蛋白質を分解
する工程、脱臭脱色した後、遠心分離器などにより液体
部分を抽出する工程を具備してなるヒアルロン酸の抽出
方法。2. A step of mincing pig skin of piglets, a step of immersing the pigskin in an alkaline solution, a step of decomposing proteins by a protease, and a step of extracting a liquid portion by a centrifugal separator after deodorization and decolorization. Method for extracting hyaluronic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP34344399A JP2001158801A (en) | 1999-12-02 | 1999-12-02 | Complex mucopolysaccharide and method for extraction thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP34344399A JP2001158801A (en) | 1999-12-02 | 1999-12-02 | Complex mucopolysaccharide and method for extraction thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2001158801A true JP2001158801A (en) | 2001-06-12 |
Family
ID=18361563
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP34344399A Pending JP2001158801A (en) | 1999-12-02 | 1999-12-02 | Complex mucopolysaccharide and method for extraction thereof |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2001158801A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004096863A1 (en) * | 2003-05-02 | 2004-11-11 | Hyun Ik Yang | Method for purification of high molecular hyaluronic acid |
-
1999
- 1999-12-02 JP JP34344399A patent/JP2001158801A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004096863A1 (en) * | 2003-05-02 | 2004-11-11 | Hyun Ik Yang | Method for purification of high molecular hyaluronic acid |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20050201 |
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