JP3996854B2 - Facial mask - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a cosmetic facial mask, and more particularly to a facial mask containing chemically reduced collagen.
[0002]
[Prior art]
Collagen facial mask for makeup contains lotion and effectively contacts active ingredients etc. to the skin, or the facial mask itself dissolves and the facial mask ingredients cover the skin, and the active ingredient specific or facial mask configuration Several types are used, such as expecting to bring about collagen specific effects. As the collagen, a freeze-dried solution made of 100% natural collagen fibers is used.
[0003]
[Patent Document 1]
JP-A-4-305600 (October 28, 1992), claim 9, [0001], [0008] and [0011]
[Patent Document 2]
Japanese Patent Laid-Open No. 2-156854 (June 15, 1990), claims (1), (Means for Solving the Problems), and Example 1
[0004]
Also, considered (also referred to as a face mask or pack agent.) Masks collagen as a component constituting the chitin, chitosan, alginic acid, although cellulose scan like are known collagen excellent in moisturizing ability as a cosmetic raw material desired ing. It is known that more specifically used collagen can be insoluble collagen or collagen solubilized by an enzyme or the like. However, since insoluble collagen is insoluble, it is difficult to purify collagen, and there is a problem that it is difficult to make the collagen to be used highly pure. Moreover, regarding the use of solubilized collagen, it is necessary to introduce intermolecular crosslinks because the physical strength of the resulting facial mask is low. However, a chemical crosslinking agent is usually used for this intermolecular crosslinking. Typical examples include aldehyde compounds, isocyanate compounds, and epoxy compounds. Although these compounds are strong and weak, the toxicity (toxicity) of any compound becomes a problem when the crosslinking agent remains. Usually, sufficient cleaning is required to prevent the cross-linking agent from remaining. However, in the case of manufacturing in large quantities, there is a problem that sufficient cleaning is not easy and time and cost are required.
[0005]
Under such circumstances, it has been proposed in Documents 1 and 2 to obtain a sheet-like collagen product with improved strength without using the disadvantageous chemical crosslinking agent. In particular, Document 1 describes providing collagen products for facial masks. Specifically, [0001] of the above-mentioned document 1 provides spongy collagen particularly suitable for use as a face mask agent, and [0008] can be used as a face mask agent. A method for producing spongy collagen that does not contain a crosslinking aid has not been successful in the past. However, the face mask described in this document has water resistance due to surface modification by 3 to 6% by weight by alkali treatment. It is described that the sheet material for use can be obtained by introducing a crosslinked structure by heat treatment. However, this method has a problem in that the manufacturing process includes a process in which chemicals that are not environmentally friendly, such as alkali treatment, must be used.
Further, in claim (1) of Document 2, a method for producing a collagen membrane characterized in that soluble collagen is reconstituted into a fibrous form and treated with a borohydride compound or the like (solves the problem). In the section of (Means for), the soluble collagen solution is reconstituted into a gel in a flat container and gelled, and then the aldol condensate and the Schiff-type crosslinks present in the collagen are reduced with a mild reducing agent. It has been described that collagen is rapidly strengthened to become a reinforced gel by stabilizing it, and it is described that a stable collagen product is obtained by reduction from soluble collagen. As well as solubilization, there is no mention of collagen other than soluble.
[0006]
[Problems to be solved by the invention]
The problem of the present invention is that a collagen dispersion that can be obtained in a large amount as a raw material but cannot be sufficiently purified, particularly a collagen tissue containing a large amount of acid solubility obtained from fish such as sharks, is mechanically disrupted. It is to provide a collagen facial mask having a sufficient physical strength using a collagen dispersion without using an unfriendly chemical or the chemical cross-linking agent.
[0007]
[Means for Solving the Problems]
The present invention reduces the unsaturated bonds present in collagen dispersions prepared from collagen-containing tissues, preferably fish tissues, more preferably fish skin tissues, preferably with sodium borohydride. And a lyophilized solution containing a collagen dispersion washed with an organic solvent, preferably chloroform / methanol, preferably 0.1% to 3.0% by weight of collagen. It is a facial mask.
Basically, the collagen unsaturated bonds are chemically reduced, preferably with sodium borohydride, specifically the reversible cross-linking sites remaining in the collagen dispersion are reduced. Thus, the above-mentioned problem could be solved.
[0008]
Furthermore, the facial mask, cellulose scan, chitin, chitosan, alginic acid, and be contained at least one kind of more selected components derivatives thereof, provided step by proteolytic enzyme of interest that loosening collagen fibers In particular, an enzyme treatment step, preferably a treatment step using an alkaline protease, can be provided before washing with the organic solvent.
[0009]
[Embodiments of the present invention]
The present invention is described in detail below.
The collagen used in the present invention is a collagen dispersion, and this collagen dispersion is not a dispersion made from soluble collagen obtained by solubilizing an enzyme, alkali or the like. That is, it is a collagen dispersion which is not subjected to a solubilization operation by mechanically disrupting a collagen tissue containing a large amount of acid solubility.
[0010]
In tissues containing collagen, collagen molecules regularly gather in a living body to form a higher-order structure, and intermolecular crosslinking exists between the molecules. This intermolecular cross-linking is an oxidative deamination reaction of a specific lysine or hydroxylysine in the telopeptide, an intermolecular cross-linking by a Schiff base by reaction of an aldehyde produced by the reaction with lysine or hydroxylysine of an adjacent collagen molecule. The intermolecular crosslinking is reversible depending on the pH, and the bond is dissociated at an acidic pH, so that the collagen is released into the acidic solution when the bond is released by the acidic solution. Since the acid-soluble collagen retains the original intermolecular cross-linking reactive group, the acid-soluble collagen is re-bonded and insolubilized by maintaining the neutral condition.
Specifically, the acid-soluble collagen having such properties is extracted in a large amount from a tissue containing collagen, for example, connective tissue such as skin, preferably fish. In the present invention, this collagen tissue dispersion containing a large amount of acid-soluble collagen contains a lot of unsaturated binding sites, and is therefore a desirable raw material for chemical reduction.
[0011]
In particular, fish such as sharks are desirable raw material species because a large amount of acid-soluble collagen can be obtained from a tissue containing collagen by acid extraction. More specifically, leather is desirable because it is easily available in large quantities as a raw material. In the case of mammals and birds, since the amount of extraction decreases with time, it is desirable to use fetuses or animal tissues within a few months of birth. Therefore, skin, tendon, etc. are desirable because of their high collagen content.
In addition, the structure | tissue said by this invention means the state which left the higher-order structure instead of solubilization which is the state which separated the collagen into the molecular form as mentioned above, and is not soluble.
[0012]
A tissue containing collagen is placed in an aqueous solution having a pH of 7 to 9, and pulverized mechanically at a temperature lower than the temperature at which the collagen is denatured to form a minced shape. Specifically, it is desirable to perform extraction at 30 ° C. or lower for mammals and birds, and at 20 ° C. or lower for fish.
[0013]
Minced (mechanically broken) collagen disperses in water. This neutral collagen in a dispersed state has many reversible intermolecular crosslinks as described above. This bond becomes an irreversible bond by chemically reducing the intermolecular bridge. Specifically, a reducing agent having a double bond as a saturated bond, sodium borohydride, lithium aluminum hydride, or the like can be used, and sodium borohydride is desirable.
A sodium borohydride in an amount of 1 to 50 times the amount of collagen is added, and the reaction is carried out at a temperature lower than that causing denaturation of the collagen for 0.5 to 3 hours. After the reaction, the insoluble collagen can be sufficiently purified.
[0014]
Although it is indispensable to manufacture the facial mask of the present invention while leaving the fiber structure, it is important to uniformly disperse the fibers in order to obtain a facial mask excellent in use feeling. For this purpose, it is necessary to disperse thick fibers to a certain degree. For this purpose, it is desirable to use a proteolytic enzyme, and this treatment is performed before the chemical reduction step described above. It is desirable. By carrying out this treatment step with an alkaline protease under alkaline conditions of pH 9-11, a dispersion liquid excellent in fiber dispersion and free of coloring and smell can be obtained. It is desirable to use the enzyme concentration within the range of 0.1 to 1% with respect to the drying of the tissue as the raw material. By this enzyme treatment step, impurities other than collagen contained in the collagen fibers, particularly odors, can be easily removed.
[0015]
Disperse the collagen after chemical reduction in water. Specifically, after adjusting to a concentration of 0.1 to 10 g / mL, washing with an organic solvent is performed. Specifically, chloroform, methanol, etc. can be mentioned. The collagen dispersion that has been washed with the organic solvent can be freeze-dried to produce a facial mask. There is no particular limitation on lyophilization, but when the dispersion is neutral, the dispersion is not viscous, so that the collagen component tends to precipitate, so it is desirable to increase the viscosity of the dispersion as slightly acidic.
The cellulose scan as another component, chitin, chitosan, alginic acid, and derivatives thereof can also be added to the dispersion of collagen. If the viscosity is increased by adding other components to the collagen dispersion, the dispersion can be neutralized.
[0016]
The facial mask of the present invention is a material having excellent physical strength, good moisture absorption, and excellent tactile sensation. EXAMPLES The present invention will be specifically described below with reference to examples, but the present invention is not limited to any examples.
[0017]
【Example】
Example 1
About 200 kg of minced (mechanically broken) shark skin is added to 0.2% sodium bicarbonate and 0.15% sodium chloride 800 L and dispersed uniformly. After confirming that the dispersion is between pH 7 and 9, 75 g of sodium borohydride is added and gently stirred for 30 minutes at room temperature. After adding hydrochloric acid to adjust the pH to 4, 40 kg of NaCl is added and left overnight. The deposited precipitate is collected by centrifugation, 100 L of methanol and 100 L of chloroform are added thereto and left overnight, and then filtered to collect the precipitate. This washing with methanol / chloroform was repeated several times, and then the precipitate was dispersed again in water and then lyophilized to obtain a facial mask.
When water was dropped on this facial mask of A4 size, it was a sponge that absorbed water droplets immediately and was excellent in water absorbability. Further, after immersing this A4 facial mask in water and picking up one end of the sponge, the sponge was not cut by its own weight.
[0018]
Example 2
Before the treatment with sodium borohydride in Example 1, 75 g of alkaline protease (Zeoplasecom manufactured by Nagase Chemtech Co., Ltd.) was added, and after gently stirring for 3 hours, the same treatment as in Example 1 was performed. I got a facial mask with no smell.
[0019]
Comparative Example 1
A sponge not subjected to sodium borohydride treatment was prepared from the manufacturing method described in the cited document 1, and after making this A4 size, this facial mask was immersed in water and one end of the sponge was picked up and lifted by its own weight. The sponge has broken.
[0020]
【The invention's effect】
As described above, the present invention brings about an excellent effect that a facial mask that is chemically safe and excellent in physical strength can be provided.

Claims (9)

Following the step of chemically reducing a uniformly dispersed unsaturated bond tissue containing mincing treated fish collagen without performing the process of solubilization, manufacture comprising a step of washing the dispersion of its by organic solvents A facial mask produced by freeze-drying a liquid containing a collagen dispersion obtained by the method. 2. The facial mask according to claim 1, wherein sodium borohydride is used in the chemical reduction. Fe Issy Yarumasuku according to claim 1 which comprises using the skin tissue as the tissue of fish. Claim 1, 2 or the facial mask according to any one of claims 3 to the use of chloroform-methanol as washing with an organic solvent and Features. According to claim 1, facial mask according to any one of claims 4 was 3 or characterized in that it contains 0.1% to 3.0% collagen as one of the at least components in the dispersion. Cellulose scan, chitin, chitosan, alginic acid, and claim 1 that contains at least one more component selected their derivatives, 3, 4 or 5 either facial mask according to the section. Claim 1 comprising the step of loosening the collagen fibrils by proteolytic enzymes, 2, Facial mask according to 6 or in the section was 3,4,5 or. The facial mask according to claim 7 , further comprising an enzyme treatment step before chemical reduction. The facial mask according to claim 8 , wherein an alkaline protease is used as the enzyme.