JP2001114659A - Composition for oral cavity - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a composition for an oral cavity capable of effectively reducing the acid production by Streptococcus mutans which is a causative bacterium of dental caries, thereby suppressing the decalcification and resultantly exhibiting excellent preventing effects on the dental caries and excellent in safety as the composition for the oral cavity. SOLUTION: This composition for the oral cavity comprises one or more kinds selected from rose bengal, phloxine, erythrocin, 2',4',5',7'- tetrabromofluorescein disodium salt and 4',5'-dibromo-2',7'-dinitrofluorescein disodium salt.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION The present invention suppresses the acid production of cariogenic bacteria, and is highly durable and highly safe.
The present invention relates to an oral composition effective for preventing dental caries.

[0002]

2. Description of the Related Art
Regarding the means for preventing dental caries, technical developments based on the etiology of dental caries have been carried out from various fields, for example, dental caries preventive agents using antibodies (Japanese Patent Laid-Open No. 60-338327),
Oral composition containing enzyme (Japanese Patent Application Laid-Open No. 56-11571)
No. 2), an oral composition containing a bactericide (Japanese Unexamined Patent Publication No.
No. 9-101417) has been proposed, but the caries preventive effect of these proposals is not yet sufficient.
The development of more effective means has been desired.

[0003] In recent years, attention has been paid to a technique from the viewpoint of preventing caries by effectively suppressing an acid having a demineralizing action.

However, up to the present, no substance has been found which can continuously suppress the acid production of cariogenic bacteria, has high safety, and can be used for oral use. It is the current situation.

The present invention has been made in view of the above circumstances, and an object of the present invention is to provide a highly safe oral composition that continuously suppresses the acid production of carious bacteria.

[0006]

Means for Solving the Problems and Embodiments of the Invention The present inventors have conducted intensive studies in order to achieve the above object, and as a result, carious bacteria have taken sugar as an energy source,
Finally, metabolism is completed by producing an acid, and sugar is taken in by utilizing energy substances in the cells (Biochim. Biophy).
s. Acta 692: 415-4241982, In
fact. Immun. 58: 1479-1480,
1990, J. Am. Dent. Res. 72 1386-
1390 1993), those that affect the uptake of sugar, an early part of sugar metabolism,
Based on the hypothesis that it will eventually lead to acid production suppression, as a result of further intensive research, we came to focus on substances having affinity for the bacterial cell surface, and when we examined various substances with such properties, It has been found that a specific dye component among food dyes and the like can adhere to carious bacterial cells, and as a result, acid production of the bacterial cells can be effectively suppressed.

That is, based on the above-mentioned hypothesis, the present inventor has conducted various studies on components that affect sugar incorporation, and as shown in Examples described later, it has been used as a dye for various uses such as a food dye. Among the ingredients, there are Rose Bengal, Phloxine, Erythrosin, 2 ',
A specific component selected from 4 ', 5', 7'-tetrabromofluorescein disodium salt and 4 ', 5'-dibromo-2', 7'-dinitrofluorescein disodium salt effectively enhances the acid production of bacterial cells. It was found that it was suppressed.

However, specific dyes such as rose bengal, phloxine, and erythrosine are used for plaque dyeing and the like, and patent applications have been filed (Japanese Patent Application Laid-Open No. 58-144).
No. 313, JP-A-60-75414, JP-A-61-2
91516, JP-A-4-500965, etc.), the gingiva after use is stained depending on the concentration used, and such staining is desirable after use as an oral composition for preventing dental caries. Therefore, it was necessary to prevent gingival staining after use.

Therefore, the present inventor has used the above-mentioned specific component as an active ingredient for suppressing the acid production of carious bacteria and to solve the problem of usability as an oral composition for preventing dental caries. In addition, as a result of further intensive research, by using the above specific component in a predetermined concentration range, it functions as an active ingredient for inhibiting acid production of carious bacterial cells, and as an oral composition for preventing dental caries. The inventors have found that there is no problem in the usability of the present invention, and have accomplished the present invention.

[0010] Accordingly, the present invention relates to rose bengal, phloxine, erythrosine, 2 ', 4', 5 ', 7'-tetrabromofluorescein disodium salt and 4', 5 '
1. one or more components selected from dibromo-2 ′, 7′-dinitrofluorescein disodium salt;
Provided is an oral composition characterized by containing from 5 × 10 ^{−8 to} 5 × 10 ⁻² mass%.

Here, the oral composition further comprises one or more active ingredients selected from caries-preventing enzymes, alkali metal monofluorophosphonates, fluorides, plaque inhibitors and antibacterial agents. Is more preferable.

Hereinafter, the present invention will be described in more detail.

[0013] The oral composition of the present invention comprises rose bengal, phloxine, erythrosin, 2 ', 4', 5 ',
7'-tetrabromofluorescein disodium salt (2 ', 4', 5 ', 7'-tetrabromofl
uorescein, disodium salt)
(Eosin Y), 4 ', 5'-dibromo-2', 7'-
Dinitrofluorescein disodium salt (4 ', 5'-
dibromo-2 ', 7'-dinitrofluo
rescein, disodium salt) (eosin B) is used as an active ingredient for inhibiting the acid production of carious bacteria, and these ingredients are used alone or in combination of two or more in a specific amount. In addition, it suppresses the acid production of cariogenic bacteria, thereby effectively preventing caries.

Here, rose bengal, phloxine and erythrosine are used as edible dyes in various foods, and 2 ', 4', 5 ', 7'-tetrabromofluorescein disodium salt, 4', 5 '-Dibromo-
2 ', 7'-Dinitrofluorescein disodium salt is also used as a vital dye, an adsorption indicator, and the like. In the case of the present invention, as described above, the specific component suppresses acid production of carious bacterial cells. In the composition for oral cavity of the present invention, the above-mentioned specific components are bonded to the surface of bacterial cells by the adsorption / adhesion properties of each component to change the environment of the surface of bacterial cells, thereby causing carious bacteria. The body is restricted from taking sugar,
As a result, it is considered that acid production is suppressed. In addition, from the experimental fact that the bacteria treated with the above-mentioned components also start growing when returned to the culture system, the above-mentioned components do not act bactericidal but affect the uptake of sugars in the cells. It is considered something.

The amount of each of the above components in the oral composition of the present invention is 2.5 × 10 ^{−8 to} 5 × with respect to the whole composition.
10 ^-2 % (% by mass, the same applies hereinafter), preferably 2.5 × 1
0 ^{-7 to} 5 × 10 ^-2 %, more preferably 5 × 10 ^{-6 to} 5 ×
10 ^-2 %, when two or more kinds are combined and blended, they are blended so that the total blended amount of each component falls within the range of the above blended amount.

If the amount is too small, the acid production of the carious cells cannot be sufficiently suppressed. If the amount is too large, the gingiva will be stained after use, causing a problem in use.

[0017] The oral composition of the present invention further comprises one or two selected from caries-preventing enzymes, alkali metal monofluorophosphonates, fluorides, plaque inhibitors and antibacterial agents.
It is more effective to mix more than one kind of active ingredient.

That is, when the above-mentioned caries-preventing enzyme is used in combination with the specific component of the present invention which suppresses the acid production of carious cells,
In addition to the suppression of the acid production of the cariogenic bacteria, the effects of suppressing the entire plaque and eliminating bacteria are added synergistically, so that caries can be more effectively prevented.

Here, examples of the above caries-preventing enzymes include dextranase, amylase, protease, mutanase, lysozyme and the like.
These can be used alone or in combination of two or more. Among these, dextranase and mutanase are more preferred.

When the above-mentioned caries-preventing enzyme is added to the oral composition of the present invention, the amount thereof is not particularly limited, and can be appropriately selected depending on the type of the caries-preventing enzyme. For example, when blending dextranase,
Usually 100 to 5000 units (u) per ml of oral composition
/ Ml oral composition) is preferred. Moreover, when mix | blending mutanase, it is 10-50 normally per 1 ml of compositions for oral cavity.
000 units (u / ml oral composition) are preferred. If the amount is less than the above range, the respective enzymatic effects may be attenuated, and if the amount exceeds the above range, an undesirable case may occur in terms of incorporation.

When the alkali metal phosphonate is added to the oral composition of the present invention, acid production of carious bacteria is suppressed, and remineralization on the tooth surface layer is promoted. Preventive synergistic effects can be expected.

Here, examples of the alkali metal monofluorophosphonate include sodium monofluorophosphate, potassium monofluorophosphate, lithium monofluorophosphate, and the like. More than one species can be used in appropriate combination.

When the above-mentioned alkali metal phosphonate is blended with the oral composition of the present invention, its blending amount is not particularly limited, but usually the concentration used in the whole composition is 0.1 to 100000 ppm. It is preferable to mix them so that Further considering practicality, especially 1
It is more preferable to mix them so as to be 0 to 1000 ppm. If it is less than 0.1 ppm, the concentration may be low and the effect of promoting remineralization may be poor, and if it exceeds 100,000 ppm, it may not be preferable from the viewpoint of mixability.

When the above-mentioned fluoride is added to the composition for oral cavity of the present invention, acid production of carious bacteria is suppressed, and remineralization in the tooth surface layer is promoted. A synergistic effect can be expected. Therefore, the fluoride is used for the same purpose as the alkali metal monofluorophosphonate, and a synergistic effect in preventing dental caries can be expected.

Examples of the fluoride include sodium fluoride, ammonium fluoride, stannous fluoride and the like, and these can be used alone or in an appropriate combination of two or more.

When the above-mentioned fluoride is added to the oral composition of the present invention, the amount thereof is not particularly limited.
It is preferred to be blended to be 0000 ppm,
Practically, it is more preferable that the concentration be 10 to 1000 ppm. If it is less than 0.1 ppm, the concentration may be low and the effect of promoting remineralization may be poor, and if it exceeds 100,000 ppm,
It may not be preferable from the viewpoint of mixability.

Further, examples of the above-mentioned plaque inhibitor include polyphenols, tannic acid, and mutastain, and examples of the antibacterial agents include chlorhexidine, cetylpyridinium chloride, and triclosan. These can be used alone or in combination of two or more.

The oral composition of the present invention may further comprise the above-mentioned plaque inhibitor,
When blending the above antibacterial agent, the blending amount is not particularly limited, and is usually blended so that the use concentration with respect to the whole composition is 0.0005 to 1%, particularly 0.005 to 0.5%. Is preferred. If it is less than 0.0005%, the plaque inhibitory action and the antibacterial action may be poor, and 1%
If it exceeds, it may not be preferable from the viewpoint of compoundability.

The oral composition of the present invention may contain, in addition to the above components, components used in ordinary oral compositions, as long as the effects of the present invention are not impaired. The amount can be a usual amount as long as the effect of the present invention is not hindered.

When the oral composition is a dentifrice, various components such as an abrasive, a binder, a thickener, a surfactant, a sweetener, a preservative, and a coloring agent are blended as the optional components. obtain.

The above-mentioned abrasives include, for example, silica-based abrasives such as precipitated silica, silica gel, aminosilicate and zirconosilicate;
Hydrates and anhydrides, calcium pyrophosphate, calcium carbonate, aluminum hydroxide, alumina, magnesium carbonate, tribasic magnesium phosphate, zeolite, zirconium silicate, synthetic resin-based abrasives and the like are preferably used.

Examples of the above thickener include glycerin,
Sorbit, propylene glycol, polyethylene glycol, xylit, maltit, lactit and the like are preferably used. As the binder, for example, sodium carboxymethylcellulose, hydroxyethylcellulose, carrageenan, sodium alginate, xanthan gum, carbopol, guar gum, montmorillonite, gelatin and the like are preferably used.

As the above-mentioned surfactant, for example, an anionic surfactant, a cationic surfactant, a nonionic surfactant and the like are used. More specifically, for example, sodium lauryl sulfate, α-olefin sulfonic acid Sodium, N-acyl sarcosinate, N-acyl glutamate, 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine, N-acyl taurate, sucrose fatty acid ester, alkylolamide, polyoxyethylene Hardened castor oil, polyglycerin fatty acid ester, pluronic, polyoxyethylene sorbitan monostearate and the like are preferably used.

Examples of the above sweetener include saccharin sodium, stevioside, stevia extract, paramethoxycinamic aldehyde, neohesperidyl dihydrochalcone, perillartin and the like. Examples of the preservative include paraoxybenzoate, sodium benzoate and the like. As the flavor, for example, terpenes such as l-menthol, carvone, anethole, limonene or derivatives thereof and the like are suitable, and as the coloring agent, for example, blue No. 1, yellow No. 4, titanium dioxide and the like are preferable. .

The use of the oral composition of the present invention is not particularly limited. For example, in addition to dentifrices such as toothpaste, liquid toothpaste and powdered toothpaste, mouthwashes, mouthwashes, oral pasta, Lozenges, chewing gum, various uses such as mouthwash, can also be mentioned, candy,
It can be used as a food or drink such as ice cream, but is not limited thereto.

The method for producing the oral composition of the present invention is not particularly limited, and the oral composition may be produced in accordance with a conventional method for each dosage form so that the above essential components are uniformly mixed in the composition. For example, a dentifrice can be produced by adding the above-mentioned active ingredient and the above-mentioned optional ingredients to the above-mentioned essential ingredients, if necessary, and mixing these ingredients with water. The dosage and usage are not particularly limited, and the normal production of each dosage form is used in a conventional manner, thereby effectively suppressing the acid production of carious bacteria and providing excellent caries prevention. The action is exerted.

[0037]

EFFECT OF THE INVENTION The oral composition of the present invention comprises S. cerevisiae, a causative causative bacterium. mutans (Streptococcus mutans) can effectively reduce acid production, thereby suppressing demineralization. As a result, an excellent caries-preventing effect is exhibited, and safety as an oral composition is achieved. Also excellent in nature. Therefore, the oral composition of the present invention can be used particularly effectively as a caries preventive agent such as a caries preventive dentifrice, an acid production suppressing mouthwash used after brushing, and a mouthwash used before going to bed. It is.

[0038]

EXAMPLES Hereinafter, the present invention will be described in more detail with reference to experimental examples and examples, but the present invention is not limited to the following examples. In addition,% in the following examples and tables shows mass%.

First, when non-metabolizable sugars and poorly metabolizable sugars incorporated into the cells coexist, acid production can be suppressed, or a stainable substance having an affinity for the membrane on the cell surface or the like can be obtained. The following Experimental Examples 1 and 2 were conducted using the substances shown in Tables 1 and 2 as test samples in order to examine whether or not there is any effect. The results are shown in Tables 1 and 2.

[Experimental Example 1] <Experiment for confirming effect of inhibiting acid production> mutans NC
The TC10449 strain was anaerobically pre-cultured in a BHI medium, and then anaerobically cultivated in the same medium. Next, after collecting cells by centrifugation, potassium chloride (KCl) buffer (pH 6.6)
Was washed. The cells were adjusted to the final turbidity of 1.4 with the same buffer, and placed in a potassium chloride buffer containing a test sample having the concentrations shown in Tables 1 and 2 and glucose at a final concentration of 15 mM. To start the reaction, and after 3 hours and 12 hours, the pH was measured.

[Experimental Example 2] <Test for confirming the appearance in the oral cavity> Each test sample was kneaded with a dentifrice composition having the following composition so as to have the concentrations shown in Tables 1 and 2, and the dentifrice was used for 3 minutes by the usual method. Polish,
After gargle normally, the oral cavity was observed, and those with a clear difference from the gingival color before toothbrushing were visually observed, and those with no clear difference were judged as ○.

Toothpaste composition component content (% by mass) aluminum hydroxide 45 sorbitol 30 sodium lauryl sulfate 0.8 sodium alginate 0.6 sodium saccharin 0.1 gelatin 0.2 lauric acid diethanolamide 1.6 propylene glycol 5 fragrance 0.3 water balance 100.0

[0043]

[Table 1]

[0044]

[Table 2]

According to the results shown in Table 1, xylitol,
Mannitol, sunset yellow, Nyukokushin,
S. While acid production by mutans cannot be suppressed, specific components of the present invention such as phloxine, erythrosine, rose bengal, and eosin Y (2 ′, 4 ′, 5 ′,
7'-tetrabromofluorescein disodium salt),
Eosin B (4 ', 5'-dibromo-2', 7'-dinitrofluorescein disodium salt) is S.E.
Mutant is found to inhibit acid production.

The specific components of the present invention contain 0.1.
Even at a low concentration of 00025%, the pH after 3 hours and 12 hours is higher than around 5.0 where demineralization is supposed to occur, and also in this experimental system, acid production is continuously suppressed for 12 hours or more. Thus, it is recognized that demineralization of the tooth surface can be prevented.

The number of cells used in the above experiment was about 1
It is the ninth power of 0. On the other hand, S. aureus in the risk of dental caries in the oral cavity. The number of bacteria per ml of mutans saliva is 10 5 in literature, and the number of bacteria in the above experiment is 10,000 times as large. When the cell turbidity is reduced to 1/100, the decrease in pH is about 100 times slower, and the pigment component concentration is correspondingly reduced by 1/100.
Since the suppression is expected to take place at a concentration of / 100,
In the above experimental results, the concentration at which acid production was suppressed is such that the concentration of acid production can be suppressed even at a concentration of 1/10000 with respect to the number of salivary bacteria in the artificial cavity.

Therefore, according to the above experimental results, the specific component of the present invention was contained in the composition in an amount of 0.00000000025%
(2.5 × 10 ⁻⁸ %), the S.C. of those at risk of being susceptible to dental caries. mutans bacteria count 10
It is recognized that even the fifth power can be covered for 12 hours or more.

On the other hand, according to an oral appearance confirmation test after use as an oral composition for preventing dental caries, when the use concentration of the specific component is 0.1%, the pigment component after use is deposited on the gingiva. At 0.025%, no deposition of the pigment component on the gingiva after use was not observed. At a use concentration of 0.05% or less, no deposition of the pigment component on the gingiva after use was observed. It was judged that it was also excellent in usability.

Further, according to the results shown in Table 2, the addition of enzymes and fluorides enhances the acid production inhibitory effect of the specific components of the present invention, phloxine, erythrosine, rose bengal, eosin Y and eosin B, It is recognized that the pH after 12 hours is more stably maintained. Therefore, when the plaque removing effect of the enzyme and the remineralizing effect of the fluoride are added to the acid production suppressing effect of the above specific component, it is expected that the effect of preventing the dental caries, which is a multifactorial disease, will be synergistically exerted.

[Experimental Example 3] <Confirmation of the growth of the pigment-treated bacterial cells in the culture system> The bacterial cells were cultured and washed in the same manner as in Experimental Example 1 above. Was adjusted to _OD550nm = 2.8. To this bacterial solution, either the specific component described above or an ordinary amount of a bactericide (chlorhexidine) in an amount to give a final concentration of 0.025% by mass is added, reacted for 3 minutes, and washed twice with the potassium chloride buffer. After that, the turbidity was again adjusted to 2.
8 and 0.1 ml of it was added to 4 ml of BHI medium
Was added. As a control, 0.1 ml of a bacterial solution treated with a potassium chloride buffer solution (a bacterial solution treated in the same manner without adding the above-mentioned specific pigment component and bactericide) was added to 4 ml of a BHI medium.
Was added. After aerobically culturing them overnight, final cell turbidity and pH were measured. Table 3 shows the results.

[0052]

[Table 3]

According to the results shown in Table 3, the final turbidity and the final pH after cultivation of both the pigment-treated cells and the buffer-treated cells were substantially equal. It can be inferred that the suppression of the decrease is not due to the bactericidal effect, but rather to the sugar uptake suppressing effect due to the change of the microenvironment of the bacterial cell surface due to the specific component bound to the bacterial cell surface.

Experimental Example 4 <S. Confirmation of hydroxyapatite (HA) powder, which is a main component of tooth enamel of mutans sugar metabolite, has a decalcifying effect> HA powder, a main component of enamel 1
0 μg of S.P. mutans 10449 sugar metabolite pH
3.3 and pH 5.1 were added in 4 μl, 40 μl, 40 μl, respectively.
When 0 μl, 2 ml, and 4 ml were added and the solubility after 3 hours was examined, the pH 3.3 solution was about 40 μl and all H
A powder was dissolved. However, the pH 5.1 solution is 2
It did not dissolve even in ml.

From the above experimental results, if the specific pigment component of the present invention suppresses the decrease in pH to about pH 5, demineralization can be sufficiently suppressed. The acid production inhibitory effect of the specific dye component leads to inhibition of dissolution of the HA powder, indicating the usefulness of the present invention. That is, it means that the above-mentioned specific pigment component has a great effect in preventing dental caries, which is essentially demineralization of enamel.

Hereinafter, examples will be described.

[Example 1] Toothpaste Aluminum hydroxide 45% by mass Sorbit 30 Sodium lauryl sulfate 0.8 Sodium alginate 0.6 Sodium saccharin 0.1 Gelatin 0.2 Lauric acid diethanolamide 1.6 Propylene glycol 5 Perfume 3 Sodium lauroyl sarcosine 0.4 Sodium monofluorophosphate 0.75 Dextranase 2000u / g Toothpaste 20u / g Toothpaste Rose Bengal 0.00005 Water Balance 100.0% by mass

[Example 2] Toothpaste Calcium carbonate 45% by mass Sorbite 24 Sodium lauryl sulfate 1.3 Carrageenan 0.7 Sodium alginate 0.3 Saccharin sodium 0.1 Gelatin 0.2 Lauric acid diethanolamide 0.8 Propylene glycol 4 Lysozyme 50u / g Toothpaste Perfume 0.8 1-Menthol 0.3 Lauroylsarcosine sodium 0.4 Sodium monofluorophosphate 0.75 Dextranase 2000u / g Toothpaste Mutanase 30u / g Toothpaste Phloxine 0.0005 Water Balance 100 0.0% by mass

Example 3 Toothpaste Dibasic calcium phosphate dihydrate 50% by mass Sodium lauryl sulfate 1 Carrageenan 0.6 Xanthan gum 0.3 Saccharin sodium 0.1 Gelatin 0.2 Lauric acid diethanolamide 1 Propylene glycol 4 Fragrance 0.8 l-menthol 0.3 lauroyl sarcosine sodium 0.4 sodium monofluorophosphate 0.5 glycerin 0.5 dextranase 3000 u / g dentifrice mutanase 20 u / g dentifrice Rose Bengal 0.0005 water balance 100. 0 mass%

Example 4 Toothpaste Silica 20% by Mass Sorbite 60 Sodium Lauryl Sulfate 0.9 Saccharin Sodium 0.15 Gelatin 0.3 Lauric Diethanolamide 1.5 Propylene Glycol 5 Flavor 1.2 l-menthol 0.3 Lauroyl sarcosine sodium 0.5 stannous fluoride 0.2 xanthan gum 0.5 polyethylene glycol # 4000 0.5 dextranase 3000u / g dentifrice mutanase 30u / g dentifrice phloxine 0.005 water balance 100.0 mass%

Example 5 Toothpaste Modified Aluminum Hydroxide 50% by Mass Sodium Lauryl Sulfate 1.5 Carrageenan 0.5 Saccharin Sodium 0.14 Propylene Glycol 5 Fragrance 1.0 1-Menthol 0.3 Myristoyl Sarcosine Sodium 0 0.3 Sodium monofluorophosphate 0.5 Tannic acid 0.05 Glycerin 20 Silicic anhydride 5 Titanium oxide 0.5 Carboxymethylcellulose sodium 0.1 Dextranase 2000u / g Toothpaste Amylase 20u / g Toothpaste Phloxine 0.0000005 Eosin Y 0.0005 water balance 100.0 mass%

Example 6 Toothpaste Modified Aluminum Hydroxide 50% by Mass Sodium Lauryl Sulfate 1.5 Carrageenan 0.5 Xanthan Gum 0.5 Saccharin Sodium 0.14 Propylene Glycol 5 Fragrance 1.0 1-Menthol 0.3 Myristoyl Sarcosine sodium 0.3 protease 20u / g dentifrice sodium fluoride 0.5 glycerin 20 anhydrous silicate 5 titanium oxide 0.5 carboxymethylcellulose sodium 0.1 dextranase 5000u / g dentifrice mutanase 30u / g dentifrice erythrosine 0.0000005 Eosin B 0.0005 water balance 100.0 mass%

Example 7 Liquid Toothpaste Silica 18% by Mass Sorvit 55 Sodium Lauryl Sulfate 1 Sodium Saccharin 0.1 Gelatin 0.2 Diethanolamide Laurate 1.2 Propylene Glycol 2 Fragrance 1.1 1-Menthol 0.3 Palmitoyl Monkey Cosin sodium 0.4 Sodium fluoride 0.2 Triclosan 0.05 Glycerin 18 Xanthan gum 0.1 Decaglyceryl laurate 0.5 Sodium sulfate (anhydrous) 0.3 Dextranase 3000u / g Toothpaste Mutanase 30u / g Toothpaste Erythrosin 0 .0005 Rose Bengal 0.005 Water balance 100.0% by mass

Example 8 Mouthwash Denatured ethanol 1.6% by mass Polyoxyethylene hydrogenated castor oil 2 Citric acid 0.01 Trisodium citrate 0.3 Perfume 0.2 1-Menthol 0.3 Laurolyl monkey Cosin sodium 0.1 Sodium fluoride 0.08 Glycerin 10 DL-alanine 1 Dextranase 4600u / g Mouthwash 50u / g Mouthwash Phloxine 0.0005 Water Balance 100.0% by mass

Example 9 Mouse Wash 90% Ethanol 20% by Mass Polyoxyethylene (80 mol) Sorbitan Monotaurate 0.5 Fragrance 1.2 1-Menthol 0.3 Sodium Monofluorophosphate 0.15 Lauroyl Monkey Cosin sodium 0.01 Dextranase 4600 u / ml Mouthwash amylase 50 u / ml Mouthwash Rose Bengal 0.0005 Erythrosin 0.005 Water Balance 100.0% by mass

Example 10 Lozenges Arabic Gum 6% by Mass Perfume 0.7 1-Menthol 0.3 Sodium Monofluorophosphate 0.05 Lauroylsarcosine Sodium 0.01 Glucose 36 Palatinose 36 Dextranase 4600u / g Troche Mutanase 50u / g troche erythrosine 0.005 rose bengal 0.005 water balance 100.0 mass%

Example 11 Chewing Gum Gum Base 20% by Mass Fragrance 0.7 1-Menthol 0.3 Sodium Monofluorophosphate 0.1 Sodium Lauroyl Sarcosine 0.01 Calcium Carbonate 2 Sugar Cane 15 Powdered Sugar 58 Dextranase 2000u / G gum mutanase 20u / g gum phloxine 0.0005 water balance 100.0 mass%

Example 12 Foam Toothpaste Sodium Lauryl Sulfate 0.2 POE (60) Hardened Castor Oil 1.0 2-Alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine 0.06 Glycerin (85 %) 10.0 Propylene glycol 1.0 Xanthan gum 0.1 Polyvinylpyrrolidone 0.5 Xylitol 1.0 Sodium fluoride 0.08 Citric acid 0.08 Sodium citrate 0.2 Sodium benzoate 0.3 Methyl paraben 0.2 Perfume 0.5 Erythrosine 0.005 Water Balance 100.0% by mass

[Examples 13 to 16] Mouthwashes The components were mixed with water so as to have the composition shown in Table 4, and mixed until the whole became uniform. Manufactured.

[0070]

[Table 4]

The effect of inhibiting the production of acid by Streptococcus mutans was examined on the oral compositions of the above Examples in the same manner as in Experimental Example 1. As a result, all the oral compositions showed good acid production inhibiting ability. . In addition, it was confirmed that all of the compositions for oral cavity of the above examples can stably incorporate food coloring and other active ingredients.

Claims (2)

[Claims] 1. Rose bengal, phloxine, erythrosine, 2 ′, 4 ′, 5 ′, 7′-tetrabromofluorescein disodium salt and 4 ′, 5′-dibromo-
One or more components selected from 2 ′, 7′-dinitrofluorescein disodium salt are added in an amount of 2.5 × 10 ⁻⁸ to 2.5 × 10 ⁻⁸ .
An oral composition characterized by containing 5 × 10 ^-2 mass%. 2. The method according to claim 1, further comprising one or more active ingredients selected from caries preventive enzymes, alkali metal monofluorophosphonates, fluorides, plaque inhibitors and antibacterial agents. Oral composition.