JP2001091821A5 - - Google Patents

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JP2001091821A5
JP2001091821A5 JP1999266690A JP26669099A JP2001091821A5 JP 2001091821 A5 JP2001091821 A5 JP 2001091821A5 JP 1999266690 A JP1999266690 A JP 1999266690A JP 26669099 A JP26669099 A JP 26669099A JP 2001091821 A5 JP2001091821 A5 JP 2001091821A5
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Japan
Prior art keywords
autofocus
light receiving
active
receiving system
observer
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JP1999266690A
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Japanese (ja)
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JP2001091821A (en
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Priority to JP26669099A priority Critical patent/JP2001091821A/en
Priority claimed from JP26669099A external-priority patent/JP2001091821A/en
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Description

【特許請求の範囲】
【請求項1】
光反射膜を施したスライドガラスまたはカバーガラスと、これらスライドガラスとカバーガラスとの間に封入された観察体を載置するステージと、このステージに載置された観察体と対峙するように配置された対物レンズと、測距光または投影光を投光し、前記スライドガラスまたはカバーガラスに施した反射膜および前記観察体に関する光学像を発生させる投光手段と、前記ステージと前記対物レンズの少なくとも一方を上下動させる駆動手段をもった顕微鏡において、
前記対物レンズを介して得られる前記反射膜に関する光学像を受光するアクティブオートフォーカス受光系と、
前記対物レンズを介して得られる前記観察体に関する光学像であるコントラスト情報を受光するパッシブオートフォーカス受光系と、
前記アクティブオートフォーカス受光系の出力に基づいて前記駆動手段を駆動し、前記反射膜に対する合焦制御を行うアクティブオートフォーカス制御手段と、
前記パッシブオートフォーカス受光系の出力に基づいて前記駆動手段を駆動し、前記観察体に対する合焦制御を行うパッシブオートフォーカス制御手段と、
前記アクティブオートフォーカス受光系の出力および前記パッシブオートフォーカス受光系の出力に基づき、前記アクティブオートフォーカス制御手段と前記パッシブオートフォーカス制御手段の何れかに切換えるオートフォーカス制御切換手段とを備えたことを特徴とする顕微鏡用オートフォーカスシステム。

【請求項2】
前記オートフォーカス制御切換手段は、前記パッシブオートフォーカス制御手段の合焦制御による前記反射膜に対するピント合わせ完了後、前記対物レンズの倍率および前記アクティブオートフォーカス制御手段による合焦制御中の前記ステージの駆動方向の何れか1つを合焦切換条件としたことを特徴とする請求項1記載の顕微鏡用オートフォーカスシステム。

【請求項3】
前記オートフォーカス制御切換手段は、オートフォーカス追従時、前記観察体に関する光学像であるコントラスト情報を合焦切換条件として、前記アクティブオートフォーカス制御手段と前記パッシブオートフォーカス制御手段との切換えを行うことを特徴とする請求項1記載の顕微鏡用オートフォーカスシステム。

【請求項4】
観察体を載置するステージと、このステージに載置された観察体と対峙するように配置された対物レンズと、測距光または投影光を投光し、前記観察体に関する光学像を発生させる投光手段と、前記ステージと前記対物レンズの少なくとも一方を上下動させる駆動手段をもった顕微鏡において、
前記測距光による前記観察体からの反射光を前記対物レンズを介して受光するアクティブオートフォーカス受光系と、
前記観察体に関する光学像であるコントラスト情報を前記対物レンズを介して受光するパッシブオートフォーカス受光系と、
前記アクティブオートフォーカス受光系の出力に基づいて前記駆動手段を駆動し、前記観察体に対する合焦制御を行うアクティブオートフォーカス制御手段と、
前記パッシブオートフォーカス受光系の出力に基づいて前記駆動手段を駆動し、前記観察体に対する合焦制御を行うパッシブオートフォーカス制御手段と、
前記アクティブオートフォーカス受光系の出力および前記パッシブオートフォーカス受光系の出力に基づき、前記アクティブオートフォーカス制御手段と前記パッシブオートフォーカス制御手段の何れかに切換えるオートフォーカス制御切換手段と
を備えたことを特徴とする顕微鏡用オートフォーカスシステム。

【請求項5】
前記オートフォーカス切換手段は、前記アクティブオートフォーカス制御手段によって前記アクティブオートフォーカス受光系の出力が所定量に達したことを検出することで、前記パッシブオートフォーカス制御手段に切換えることを特徴とする請求項4記載の顕微鏡用オートフォーカスシステム。
[Claims]
[Claim 1]
A slide glass or cover glass provided with a light-reflecting film, a stage on which an observation body enclosed between the slide glass and the cover glass is placed, and an observation body placed on this stage are arranged so as to face each other. The objective lens, the light projecting means for projecting the distance measuring light or the projected light to generate an optical image of the reflecting film applied to the slide glass or the cover glass, and the observer, and the stage and the objective lens. In a microscope with a driving means that moves at least one up and down
An active autofocus light receiving system that receives an optical image of the reflective film obtained through the objective lens, and an active autofocus light receiving system.
A passive autofocus light receiving system that receives contrast information that is an optical image of the observer obtained through the objective lens.
An active autofocus control means that drives the drive means based on the output of the active autofocus light receiving system and controls focusing on the reflective film.
A passive autofocus control means that drives the drive means based on the output of the passive autofocus light receiving system and controls focusing on the observer.
Based on the output of the active autofocus light receiving system and the output of the passive autofocus light receiving system, the autofocus control switching means for switching to either the active autofocus control means or the passive autofocus control means is provided. Autofocus system for microscopes.

2.
The autofocus control switching means drives the stage during focusing control by the active autofocus control means and the magnification of the objective lens after the focus control of the passive autofocus control means completes focusing on the reflective film. The autofocus system for a microscope according to claim 1, wherein any one of the directions is set as a focusing switching condition.

3.
The autofocus control switching means switches between the active autofocus control means and the passive autofocus control means when the autofocus tracking means, using contrast information which is an optical image of the observer as a focusing switching condition. The autofocus system for a microscope according to claim 1.

4.
A stage on which the observer is placed, an objective lens arranged so as to face the observer mounted on the stage, and distance measuring light or projected light are projected to generate an optical image of the observer. In a microscope having a light projecting means and a driving means for moving at least one of the stage and the objective lens up and down.
An active autofocus light receiving system that receives reflected light from the observer body by the distance measuring light through the objective lens, and an active autofocus light receiving system.
A passive autofocus light receiving system that receives contrast information, which is an optical image of the observer, via the objective lens.
An active autofocus control means that drives the drive means based on the output of the active autofocus light receiving system and controls focusing on the observer.
A passive autofocus control means that drives the drive means based on the output of the passive autofocus light receiving system and controls focusing on the observer.
An autofocus control switching means for switching to either the active autofocus control means or the passive autofocus control means based on the output of the active autofocus light receiving system and the output of the passive autofocus light receiving system.
An autofocus system for microscopes, which is characterized by being equipped with.

5.
The autofocus switching means is characterized in that it switches to the passive autofocus control means by detecting that the output of the active autofocus light receiving system has reached a predetermined amount by the active autofocus control means. 4. The autofocus system for a microscope according to 4.

【0016】
【課題を解決するための手段】
上記課題を解決するために、本発明に係る顕微鏡用オートフォーカスシステムは、観察体を載置するステージと、このステージに載置された観察体と対峙するように配置された対物レンズと、測距光または投影光を投光し、観察体に関する光学像を発生させる投光手段と、ステージと対物レンズの少なくとも一方を上下動させる駆動手段をもった顕微鏡に、測距光による観察体からの反射光を対物レンズを介して受光するアクティブオートフォーカス受光系と、観察体に関する光学像であるコントラスト情報を対物レンズを介して受光するパッシブオートフォーカス受光系と、アクティブオートフォーカス受光系の出力に基づいて駆動手段を駆動し、観察体に対する合焦制御を行うアクティブオートフォーカス制御手段と、パッシブオートフォーカス受光系の出力に基づいて駆動手段を駆動し、観察体に対する合焦制御を行うパッシブオートフォーカス制御手段と、アクティブオートフォーカス受光系の出力およびパッシブオートフォーカス受光系の出力に基づき、アクティブオートフォーカス制御手段とパッシブオートフォーカス制御手段の何れかに切換えるオートフォーカス制御切換手段とを備えた構成である。
0016.
[Means for solving problems]
In order to solve the above problems, the autofocus system for a microscope according to the present invention measures a stage on which an observer is placed and an objective lens arranged so as to face the observer mounted on the stage. A microscope equipped with a light projecting means that projects distance light or projected light to generate an optical image of the observer and a driving means that moves at least one of the stage and the objective lens up and down, from the observer by distance measurement light. Based on the output of an active autofocus light receiving system that receives reflected light through an objective lens, a passive autofocus light receiving system that receives contrast information that is an optical image of an observer through an objective lens, and an active autofocus light receiving system. Active autofocus control means that drives the drive means to control the focus on the observer, and passive autofocus control that drives the drive means based on the output of the passive autofocus light receiving system to control the focus on the observer. The configuration includes means and an autofocus control switching means for switching to either an active autofocus control means or a passive autofocus control means based on the output of the active autofocus light receiving system and the output of the passive autofocus light receiving system.

本発明は、以上のような構成としたことにより、アクティブAF制御手段では、アクティブAF受光系にて取込んだ観察体からの反射光の受光出力から高速度に観察体にピント合わせ可能であり、一方、パッシブAF制御手段では、パッシブAF受光系にて取込んだ標本に関するコントラスト情報から精度良く標本にピント合わせ可能であるので、AF制御切換手段では、適宜な合焦切換条件のもとにアクティブAF制御手段とパッシブAF制御手段とを切換えてAF制御を行えば、高速度、かつ、高精度に標本にピント合わせ可能である。
With the above configuration, the active AF control means can focus on the observer at high speed from the light receiving output of the reflected light from the observer captured by the active AF light receiving system. On the other hand, in the passive AF control means, it is possible to accurately focus on the sample from the contrast information about the sample captured by the passive AF light receiving system. Therefore, in the AF control switching means, under appropriate focusing switching conditions. If AF control is performed by switching between the active AF control means and the passive AF control means, it is possible to focus on the sample at high speed and with high accuracy.

(1) アクティブAF制御手段の合焦制御による前記反射膜に対するピント合わせ完了。
(1) Focusing on the reflective film is completed by focusing control of the active AF control means.

JP26669099A 1999-09-21 1999-09-21 Autofocusing system for microscope Pending JP2001091821A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP26669099A JP2001091821A (en) 1999-09-21 1999-09-21 Autofocusing system for microscope

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP26669099A JP2001091821A (en) 1999-09-21 1999-09-21 Autofocusing system for microscope

Publications (2)

Publication Number Publication Date
JP2001091821A JP2001091821A (en) 2001-04-06
JP2001091821A5 true JP2001091821A5 (en) 2006-11-02

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JP2002341234A (en) * 2001-05-17 2002-11-27 Olympus Optical Co Ltd Automatic focusing device for microscope
JP5199407B2 (en) * 2003-09-29 2013-05-15 オリンパス株式会社 Microscope system and observation method
US7345814B2 (en) 2003-09-29 2008-03-18 Olympus Corporation Microscope system and microscope focus maintaining device for the same
JP4847690B2 (en) * 2003-09-29 2011-12-28 オリンパス株式会社 Microscope system
JP2006317428A (en) * 2005-04-12 2006-11-24 Nikon Corp Face position detector
JP5108627B2 (en) * 2008-05-23 2012-12-26 オリンパス株式会社 Observation apparatus and observation method
JP5914242B2 (en) * 2012-08-08 2016-05-11 浜松ホトニクス株式会社 Observation device
JP2015108753A (en) * 2013-12-05 2015-06-11 京セラ株式会社 Focusing method and imaging device
EP4303639A3 (en) * 2014-05-30 2024-02-28 Nikon Corporation Focusing device, microscope device, focusing method, and control program
JP7034280B2 (en) * 2018-06-20 2022-03-11 株式会社日立ハイテク Observation device
JP6822535B2 (en) * 2019-09-17 2021-01-27 株式会社ニコン Focus control device, microscope device, focus control method, and control program

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Publication number Priority date Publication date Assignee Title
JPH0246416A (en) * 1988-08-06 1990-02-15 Canon Inc Focus detector
DE3828381C2 (en) * 1988-08-20 1997-09-11 Zeiss Carl Fa Method and device for automatically focusing an optical system
JP2863551B2 (en) * 1989-06-14 1999-03-03 オリンパス光学工業株式会社 Camera with automatic focusing device
JP3349711B2 (en) * 1991-03-29 2002-11-25 オリンパス光学工業株式会社 Automatic focus detection device
JPH1183474A (en) * 1997-09-12 1999-03-26 Canon Inc Distance measuring device
JPH11211468A (en) * 1998-01-29 1999-08-06 Canon Inc Range finding device and optical equipment

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