JP2001088098A - Micro chemical device with depressurized liquid feeding mechanism - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a micro chemical device eliminating the need of a number of liquid feeding means when a number of micro chemical devices are disposed in parallel with each other for reaction, analysis, and inspection and operated simultaneously. SOLUTION: A capillary flow path is formed between two members connected to each other, and a depressurization chamber is provided in connection with the flow path. The depressurization chamber is formed in a member different from the member having the capillary flow path, and these members are fixedly connected to each other.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION The present invention relates to a chemical, biochemical or physical chemistry in which a fine chemical device, that is, a structure in which members such as a fine flow path, a reaction tank, an electrophoresis column, and a membrane separation mechanism are formed. Microreaction devices (microreactors), microanalysis (including diagnosis and testing) devices such as integrated DNA analysis devices, microelectrophoresis devices, microchromatography devices, mass spectra and liquid chromatography More specifically, the present invention relates to a microchemical device for preparing an analytical sample, and more particularly, to a microchemical device having a capillary-shaped flow path formed by bonding a member having a groove-shaped flow path on its surface to another member, and sending liquid. The present invention relates to a microchemical device having a mechanism.

[0002]

2. Description of the Related Art "Science" (1998, No. 282)
Volume, p. 484), a thin groove is formed in a base material such as silicon, quartz, glass, or a polymer by an etching method to form a liquid flow path or a separation gel channel. A microchemical device in which a cover is used by adhering to a surface of the cover is disclosed. When a liquid is allowed to flow through such a device, a method has been adopted in which the liquid is pumped by a micro syringe, a microfluidic pump, or the like.

[0003]

However, when a large number of such microchemical devices are installed in parallel and operated at the same time, for example, when the microchemical device is used as a clinical diagnostic agent, a microsyringe or a small amount of microchemical devices can be used. There is a disadvantage that many liquid feeding means such as a liquid pump are required.

[0004] The problem to be solved by the present invention is to provide a microchemical device which does not require a large number of liquid sending means when a large number of microchemical devices are installed in parallel and operated simultaneously.

[0005]

Means for Solving the Problems The present inventors have conducted intensive studies on a method for solving the above-mentioned problems, and as a result, have found that the diameter is 1 to 1.
In a microchemical device for flowing a liquid through a flow path of about 000 μm, it has been found that it is preferable to use a suction force of a liquid to a decompression chamber provided in the microchemical device as a driving force for liquid transfer, and completed the present invention. I came to.

That is, in order to solve the above-mentioned problems, the present invention provides (1) (a) filling a solid substance between a member (A) and a member (B) except for a portion serving as a flow path. By
Alternatively, (b) by bonding another member (B) to the grooved surface of the member (A) having the groove on the surface, the member (A) and the member (B) bonded to each other A microchemical device having a capillary channel having a width of 1 to 1000 μm and a depth of 1 to 1000 μm as viewed from a direction perpendicular to the bonding surface between the member (A) and the member (B), In contact therewith, a microchemical device having a decompression chamber capable of decompression within the microchemical device (hereinafter referred to as the first microchemical device of the present invention) is provided.

In order to solve the above-mentioned problems, the present invention provides (2) a micro-decompression device according to (1), wherein a decompression chamber is provided between the members (A) and (B). Provide chemical devices.

According to another aspect of the present invention, there is provided a microchemical device according to the above (1) or (2), wherein (3) an exhaust port having a check valve is provided in the decompression chamber. provide.

In order to solve the above problems, the present invention provides (4) the above (1), (2) or (3) wherein the member (A) and the member (B) are formed of an organic high molecular polymer. The present invention provides a microchemical device according to the item (1).

In order to solve the above problems, the present invention provides (5) a member (A) and a member (B) each of which is composed of a styrene polymer, a (meth) acrylic polymer, a polycarbonate polymer, and a polysulfone. The present invention provides the microchemical device according to the above (4), which is formed from a polymer selected from the group consisting of a system polymer, a polyester polymer, and a vinyl chloride polymer.

In order to solve the above-mentioned problems, the present invention provides (6) a bonding surface between the members (A) and (B) between the members (A) and (B) bonded to each other. 1 to 1000 µm wide and 1 to 1000 µ deep when viewed from the direction perpendicular to
m is a microchemical device comprising a member (I) in which a capillary flow path is formed and a member (II) in which a decompression chamber is provided, wherein the pressure of the member (II) is reduced in the flow path of the member (I). Provided is a microchemical device in which the member (II) is fixed to the member (I) so that the chambers communicate with each other (hereinafter, referred to as a second microchemical device of the present invention).

The present invention further provides (7) the microchemical device according to (6), wherein the member (I) and the member (II) are detachable.

In order to solve the above-mentioned problems, the present invention provides (8) a microchemical device as described in (6) or (7), wherein an exhaust port having a check valve is provided in the decompression chamber. provide.

Furthermore, in order to solve the above-mentioned problems, the present invention provides (9) the above (6), (7) or (7) wherein the member (A) and the member (B) are formed of an organic high molecular polymer. 8)
And a microchemical device according to (1).

Further, in order to solve the above-mentioned problems, according to the present invention, (10) the member (A) and the member (B) are each composed of a styrene polymer, a (meth) acrylic polymer, a polycarbonate polymer, The microchemical device according to the above (6), (7) or (8), comprising a polymer selected from the group consisting of a polysulfone-based polymer, a polyester-based polymer, and a vinyl chloride-based polymer.

[0016]

BEST MODE FOR CARRYING OUT THE INVENTION In the first microchemical device of the present invention, a capillary channel (hereinafter simply referred to as a "channel") is provided between a member (A) and a member (B) bonded to each other. ) Is formed. The flow path may be formed by filling a portion other than the flow path between (a) the member (A) and the member (B) with a solid substance, for example, or (b) Another member (B) may be bonded to the grooved surface of the member (A) having the groove on the surface. The flow path in the above (A) has a member (A) on the bottom surface when the member (B) is up, a solid substance filled on the side surface,
The upper surface is composed of the member (B), and the flow path in the above (b) has a member (A) on the bottom and side surfaces and a member (B) on the upper surface.
Alternatively, it is composed of an adhesive applied to the member (B). In the above (a), since the member (A) and the member (B) are bonded by the solid substance filled between the member (A) and the member (B), the member (A) It can also be said that the member (B) and the member (B) are bonded with an adhesive having a space in a flow path shape.

The width of the flow path viewed from a direction perpendicular to the bonding surface between the member (A) and the member (B) is 1 μm or more, preferably 10 μm or more, and 1000 μm or less;
Preferably it is 500 μm or less. The depth of the flow path viewed from a direction perpendicular to the bonding surface between the member (A) and the member (B) is 1
μm or more, preferably 10 μm or more, and 1000 μm or less, and preferably 500 μm or less. If the flow path is smaller than these dimensions, the flow rate will be too low and the production will be difficult. Further, when the flow path is larger than these dimensions, the effect of the present invention tends to decrease, which is not preferable. The width / depth ratio of the flow channel can be arbitrarily set according to the use and purpose, but is generally preferably 0.5 to 10, and more preferably 0.7 to 5. The cross-sectional shape of the channel is arbitrary, such as a rectangle (including a rectangle with rounded corners; the same applies hereinafter), a trapezoid, a circle, a semicircle. In the present invention, the width of the flow channel refers to the maximum width of the cross section of the flow channel. The width of the channel need not be constant.

The shape of the flow path viewed from the direction perpendicular to the bonding surface of the member (A) and the member (B) may be a straight line, a branch, a comb, a curve, a spiral, a zigzag, or any other shape depending on the purpose of use. It may be. In addition to the flow path, the flow path can be used as a reaction field, a mixing field, an extraction field, a separation field, a flow rate measurement unit, a detection unit, and the like. A tank, a reaction tank, a membrane separation mechanism, a connection port outside the device, and the like may be formed.

In the case where the flow path has a structure in which a portion other than the flow path between the member (A) and the member (B) is formed by filling the solid material, the thickness of the solid material is not necessarily uniform. Although it is not, it is preferable that it is uniform.

When the flow path is formed by bonding another member (B) to the grooved surface of the member (A) having a groove on the surface, the groove is lower than its peripheral portion, that is, as a so-called groove. It may be formed, or may be formed between walls standing on the surface of the member (A). The method of providing a groove on the surface of the member (A) is arbitrary. For example, injection molding, solvent casting, melt replica method, cutting, etching, photolithography (including energy beam lithography), etching, vapor deposition, vapor deposition, A method such as a phase polymerization method and a method of bonding a sheet-like member and a plate-like member cut out from a portion to be a groove can be used. The member (A) may be made of a plurality of materials, and for example, may be made of a material whose bottom and side surfaces are different from each other. The member (A) may be provided with a structural portion other than the groove, for example, a structure serving as a liquid storage tank, a reaction tank, an analysis mechanism, or the like.

The shape of the member (A) does not need to be particularly limited, and may take a shape according to the purpose of use. For example, sheet-like (including film, ribbon, etc .; the same applies hereinafter), plate-like,
Although it may be a coating film, a rod, a tube, or a molded product having a complicated shape, the bonding surface is preferably a flat shape from the viewpoint of easy adhesion to the member (B), and a sheet, a plate, Or it is especially preferable that it is rod-shaped. When the member (A) has a groove on the surface, the surface on which the groove is formed preferably has a planar shape. The member (A) may be in a form integrated with another member, for example, a support. When the member (A) is in the form of a coating film, it is used in a state of being integrated with the support. The material and shape of the support are also arbitrary, and for example, the material and shape shown in the case of the member (A) may be used. Multiple microchemical devices in one
It is also possible to form them on one member (A), or to cut them after production to form a plurality of microchemical devices.

The member (B) is filled with a solid substance between the member (A) and the member (B) except for a portion serving as a flow path, so that the member (A) and the member (B) are solid-state. A substance capable of forming a capillary channel with a substance, or a substance having a groove on its surface (A) is adhered to the grooved surface of the member (A), and the groove of the member (A) and the member (B) are adhered. The shape, structure, surface condition, and the like are arbitrary as long as a capillary flow path can be formed. These are the same as in the case of the member (A). The member (B) does not need to have a groove formed on the surface, but may have a groove or a structure other than the groove. For example, the member (B) may be a mirror image of the member (A) having a groove formed on the surface.
When the member (B) is bonded on the member (A) having the groove formed thereon using the energy ray-curable compound as an adhesive, and the energy beam used by the member (A) is not transmitted. The member (B) needs to transmit the energy beam to be used.

A method of forming a structure in which a flow path is formed by filling a portion other than the flow path between the member (A) and the member (B) with a solid substance is described in, for example, the member (A) and the member ( An energy-ray-curable composition is sandwiched between B), and energy rays are irradiated from the outside of the member (A) and / or the member (B) except for a portion serving as a flow path, and uncured energy-ray curing is performed. Method of removing the conductive composition, method of sandwiching an adhesive sheet-like member cut out of a part to be a flow path between members (A) and (B) and bonding them together, protection of a part to be a flow path A method of removing a protective substance after filling and solidifying a substance, for example, a rod-like substance made of tetrafluoroethylene, filling and solidifying an adhesive or a molten resin, or the like can be adopted. Although this method has a small number of steps, it is difficult to remove the uncured energy-ray-curable composition and the protective substance when the flow path diameter is small, and thus it is suitable as a method for forming a flow path having relatively large dimensions. .

When the member (A) has a groove on the surface, the method of bonding the member (A) and the member (B) is such that the groove on the surface of the member (A) is formed as a flow path. It is optional, use of a solvent type adhesive, use of a solventless type adhesive, use of a melt type adhesive, adhesion by applying a solvent to the surface of the member (A) and / or member (B), heat or ultrasonic waves Can be used, but the use of a solventless adhesive is preferable, and a method of using an energy ray-curable resin as the solventless adhesive, curing by energy beam irradiation, and bonding is a small device. This is preferable because it enables precise bonding of the particles and has high productivity. Further, it is also possible to adopt a method in which the groove is filled with a protective material such as a liquid, a gel, or a solid and adhered, and then the protective material is removed. The member (B) may be a cured product of the adhesive itself.

The material of the member (A) is arbitrary, and may be an organic high molecular polymer (hereinafter, simply referred to as “polymer”), glass,
Crystals such as quartz, ceramics, metals, and semiconductors such as silicon may be used, but polymers are preferable from the viewpoint of ease of molding.

The polymer used for the member (A) may be a thermoplastic polymer or a thermosetting polymer.
Thermoplastic polymers are preferred in terms of good moldability.In addition, when grooves are formed on the surface, an energy beam-curable cross-linking layer is formed in terms of easy formation of grooves, high curing speed, and easy surface hydrophilicity. Coalescence is preferred. The member (A) may be composed of a polymer blend or a polymer alloy, or may be a composite or a laminate.

Examples of the polymer which can be preferably used for the member (A) include styrene-based polymers such as polystyrene, high-impact polystyrene, poly-α-methylstyrene, polystyrene / maleic acid copolymer, and polystyrene / acrylonitrile copolymer. Polysulfone polymers such as porsulfone and polyethersulfone; poly (meth) acrylic polymers such as polymethyl methacrylate and polyacrylonitrile; polymaleimide polymers;
Polycarbonate polymers; Cellulose polymers such as cellulose acetate and methyl cellulose; Polyurethane polymers; Chlorine polymers such as vinyl chloride and vinylidene chloride; Polyamide polymers; Polyimide polymers; Polyolefins such as polyethylene and polypropylene Polymer; polyether-based or polythioether-based polymer such as polyphenylene oxide or polyphenylene sulfide; polyester-based polymer such as polyethylene terephthalate or polyarylate, polytetrafluoroethylene, perfluoroalkoxytrifluoroethylene-tetrafluoroethylene Fluorine-based polymers such as polymers (PFA) are exemplified.

As the energy ray-curable crosslinked polymer, a cured product of an energy ray-curable compound having a (meth) acryloyl group and a cured product of an energy ray-curable compound having a maleimide group are preferable. Of course, the polymer may be a homopolymer or a copolymer.

Of these, styrene-based polymers, (meth) acrylic-based polymers, polycarbonate-based polymers, polysulfone-based polymers, and vinyl chloride-based polymers are inexpensive, have good moldability, and have good water resistance and dimensional stability. It is particularly suitable as a material for the member (A) because of its excellent physical properties such as.

The material of the member (B) in the present invention is arbitrary, and those shown as materials usable for the member (A) of the present invention can be used. The material of the member (B) may be the same as or different from that of the member (A).

The first microchemical device according to the present invention comprises:
A decompression chamber is provided in the microchemical device so as to be connected to a flow path formed between the member (A) and the member (B). The decompression chamber and the flow path may be directly connected, or may be connected via a communication flow path.

The lower limit of the volume of the decompression chamber is preferably at least 3 times, more preferably at least 10 times, the total volume from the liquid inflow section to the liquid outflow section of the flow path. The upper limit is preferably 10,000 times or less the total volume of the flow path in the microchemical device of the present invention,
More preferably, it is 1000 times or less. However, the total volume from the liquid inflow portion to the liquid outflow portion of the flow channel here is the total volume of the flow channel, and when a liquid storage tank or other structure connected to the flow channel is provided. , Not including its volume. If the volume of the decompression chamber is less than this value, the amount of liquid that can flow through the flow path is reduced, so that the use is limited.In addition, the degree of decompression tends to decrease due to leakage of air from a check valve or the like. Becomes If the volume of the decompression chamber is larger than this value, there is no problem in function, but it is not preferable because a microchemical device becomes unnecessary and large.

The installation position of the decompression chamber is arbitrary. The decompression chamber may be provided, for example, inside the member (A) or the member (B), or may be provided between the member (A) and the member (B). ) And the member (B) are preferable because the production is easy. When it is provided between the member (A) and the member (B), it can be formed simultaneously with the channel by the same method as the channel of the present microchemical device.

The decompression chamber is provided with an exhaust port for decompressing the decompression chamber and a mechanism for maintaining the decompression. As a mechanism for maintaining the reduced pressure in the decompression chamber, for example, an opening / closing valve, a mechanism for closing an exhaust port with a clamp, a portion to be melt-sealed or adhesively sealed, a check valve, a stopper, or a rubber for air release by a syringe And the like. The pressure reducing chamber may be provided with a leak valve mechanism for releasing the pressure reduction. When the decompression chamber is used as a sample storage tank, a structure that facilitates the use of the sample liquid, such as a rubber part for extracting a sample by a microsyringe, may be provided. Further, the decompression chamber may be divided into a sample storage tank portion and other portions. The shape and internal structure of the decompression chamber are arbitrary. For example, the decompression chamber may have ribs or columns for preventing deformation of the decompression chamber, or the decompression chamber may be porous.

In the microchemical device of the present invention, the flow path may be branched, and other functional parts such as a liquid storage tank, a reaction tank, and an electrophoresis column may be connected to an arbitrary position of the flow path. , An electrode, a chromatographic column, and the like. These may be provided on the member (A), may be provided on the member (B), or may be provided on the member (B) or another member coupled to the member (A). May be. A decompression chamber may be provided in each of the branched flow paths.

The second microchemical device according to the present invention comprises:
A capillary having a width of 1 to 1000 μm and a depth of 1 to 1000 μm between the member (A) and the member (B) bonded to each other when viewed from a direction perpendicular to the bonding surface of the member (A) and the member (B). And a member (II) provided with a decompression chamber.

The members (A) and (B) constituting the member (I), forming a flow path between the members (A) and (B) by bonding them, Regarding the structure of (1), the description is the same as that of the first microchemical device according to the present invention, except that the member (I) does not need to have a decompression chamber.

[0038] The second microchemical device of the present invention comprises:
The member (II) is fixed to the member (I) so that the decompression chamber of the member (II) communicates with the flow path of the member (I); and
The volume of the decompression chamber is 10 to 10 times the volume of the flow path of the member (I).
It differs from the first microchemical device of the present invention in that it is in the range of 00000 times. That is, in the second microchemical device of the present invention, the liquid absorbing portion is formed on a member (II) different from the member (I) and is connected to the flow path of the member (I). This connection may be a direct connection or a connection via a communication channel. The member (II) has a decompression chamber, and the decompression chamber is the member (I)
The structure, the shape, and the positional relationship with the member (I) are arbitrary as long as the shape can be connected to the channel. For example, the member (I
I) may be provided on an extension of the flow path in the same plane as the member (I), or the flow path of the member (A) and / or the member (B) of the member (I) may be formed. It may be provided on a surface opposite to the surface provided, and may be connected to the flow path of the member (I) by a communication flow path penetrating the member (A) and / or the member (B).

In the second microchemical device of the present invention, the lower limit of the volume of the decompression chamber is at least 10 times the total volume from the liquid inflow portion to the liquid outflow portion of the flow path of the member (I). And more preferably 100 times or more. The upper limit is preferably 1,000,000 times or less, more preferably 10,000 times or less, and most preferably 1,000 times or less, of the total volume of the flow path in the microchemical device of the present invention. If the volume of the decompression chamber is less than this value, the amount of liquid that can flow through the flow path is reduced, so the use is limited, and the degree of decompression is reduced due to air leakage from a check valve or the like. Tends to be.
If the volume of the decompression chamber is larger than this value, there is no problem in function, but it is not preferable because the member (II) becomes unnecessarily large. The second microchemical device according to the present invention is suitable for use in flowing a relatively large amount of liquid, such as a chemical or biochemical synthesis device.

In the case of using the device for flowing a larger amount of liquid, when the member (I) of the microchemical device is repeatedly used, or when sampling the liquid, the member (II) is used to facilitate these. And the member (I) are preferably detachable. Detachable mechanism is optional, for example, claw type, bayonet type, screw-in type, screwing,
It can be fixed with an adhesive type, a magic tape, rubber fastening, other fasteners, etc., but a claw type is preferable. In order to hermetically connect the flow path and the decompression section to other portions, it is preferable to use an O-ring or packing for the connection section.

Other aspects of the decompression chamber, such as the shape, internal structure, exhaust port, check valve, and other auxiliary structures, are the same as in the case of the first microchemical device of the present invention. A member for each of the branched channels (II)
May be connected.

The material constituting the member (II) is also arbitrary, and any material that can be used for the member (A) or the member (B) can be used.

[0043]

EXAMPLES Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited to the scope of these examples. In the following examples, "part"
Represents "parts by weight" unless otherwise specified.

<Preparation of energy ray-curable composition> A method for preparing an energy ray-curable composition used in Examples will be described.

[Preparation of energy ray-curable composition [e1]] Urethane acrylate oligomer having an average of three acrylic groups per molecule ("Unidick V-4263" manufactured by Dainippon Ink and Chemicals, Inc.) 40 parts, 60 parts of nonylphenoxy polyethylene glycol (n = 8) acrylate (“M-114” manufactured by Toa Gosei Chemical Co., Ltd.) and 1-hydroxycyclohexyl phenyl ketone (“Irgacure 18” manufactured by Ciba Geigy) as an ultraviolet polymerization initiator
4 ") 2 parts were mixed to form an energy ray-curable composition [e
1] was prepared.

[Preparation of energy ray-curable composition [e2]] In the above-mentioned preparation of the energy ray-curable composition [e1], the ultraviolet ray polymerization initiator "Irgacure 184" was used.
And the polymerization inhibitor 2,4-diphenyl-4-methyl-1-pentene (manufactured by Kanto Chemical Co., Ltd.).
Except for adding 1 part, the energy ray-curable composition [e
Energy ray-curable composition [e2] in the same manner as in [1].
Was prepared.

[Example 1] [Preparation of member (A)] A 2.5 cm x 5 cm x 3 mm plate-like base made of polystyrene ("Dick Styrene XC-520" manufactured by Dainippon Ink and Chemicals, Inc.) After applying the energy ray-curable composition [e1] to the material (1) using a 127 μm bar coater, the pressure-reducing chamber (5) shown in FIG.
Is covered with a photomask, and irradiated with 50 mW / cm ² ultraviolet rays for 30 seconds in a nitrogen atmosphere using a light source unit for a Multilight 200 type exposure apparatus manufactured by Ushio Inc. e1] A cured product layer (2) was formed, and uncured portions were removed with a stream of water. Next, the energy ray-curable composition [e2] is applied thereon using a 50 μm bar coater, and the flow path (4) and the decompression chamber (5) having the shape shown in FIG. It was covered with a mask and irradiated with the same ultraviolet rays as above in a nitrogen atmosphere for 30 seconds. After irradiating with ultraviolet rays, the uncured material is washed and removed with a surfactant aqueous solution, so that the cured material layer (3) made of the energy ray-curable composition [e2] has a width of 33 μm, a depth of 38 μm, A groove to be a flow path (4) having a length of 6 cm, a cured product layer (2) composed of the energy ray-curable composition [e1] and an energy ray-curable composition [e
2] as a defect in both layers of the cured product layer (3)
A square recess having a square shape of 0 mm, a length of 10 mm, and a depth of 140 μm was formed. Then, a drill hole having a diameter of 3 mm was drilled at the end of the groove to become the flow path (4) with a drill to make the inlet (6), and the diameter of 0 was drilled in a part of the concave part to become the decompression chamber (5). A check valve (7) and an exhaust port (8) were provided in a 0.5 mm drill hole to obtain a member [A-1].

[Adhesion of member (B)] The same polystyrene plate (1) as polystyrene plate (9) [B-1]
The energy ray-curable composition [e1] was applied using a 7 μm bar coater, and then irradiated with the same ultraviolet ray for 1 second in a nitrogen atmosphere, and the coating film lost fluidity but was incompletely cured. And the surface of the coating film is referred to as a member [A-
1), the same ultraviolet ray is further irradiated for 30 seconds through a polystyrene plate (9) to completely cure the coating film, and the polystyrene plate (9) and the energy ray-curable composition are adhered to each other. [E1] A plate-like member [B-1] made of an adhesive of the cured product (10) is adhered to the surface of the member [A-1], and a capillary channel (4) is formed between them. The decompression chamber (5) was formed, and the microchemical device [D-1] having the shape shown in FIG. 1 was manufactured.

[Liquid sending test] Microchemical device [D-
1] is placed with the member (A) side up, that is, the inlet (6) side up, and distilled water colored with methylene blue (manufactured by Kanto Chemical Co., Ltd.) is poured into the inlet (6) using a pipette. When one drop was injected and the air at the exhaust port (8) was removed by an aspirator, the water continued to flow along the flow path (4) at a substantially constant flow rate, and the whole amount flowed into the decompression chamber (5).

[Example 2] [Preparation of micro-micro device] In Example 1, the pressure reducing chamber (5) shown in FIG.
Except for having no, a member (I) similar to the microchemical device [D-1] of Example 1 was prepared in the same manner as in Example 1, and connected to the exhaust port (8) of the member (I). Outside diameter 3
mm, soft vinyl chloride tube of 2 mm inside diameter (not shown)
Except that [Member (II)] was provided as a decompression chamber and a fusion sealing portion, the members (I) and (I) were formed in the same manner as in Example 1.
A microchemical device [D-2] consisting of (I) was prepared.

[Liquid sending test] Microchemical device [D-
2] is placed on the member (A) side, that is, with the inlet (6) side up, and distilled water colored with methylene blue (manufactured by Kanto Chemical Co., Ltd.) using an inlet (6) with a pipette. Is dropped, and while the pressure is reduced by an aspirator through a vinyl chloride tube (not shown) connected to the exhaust port (8), the vinyl chloride tube is fused and cut by heat leaving 5 cm of the vinyl chloride tube. When the inside was kept at a reduced pressure, the water passed through the flow path (4), continuously flowed at a substantially constant flow rate, and flowed into a reduced-pressure chamber (not shown) in the vinyl chloride tube in which the entire amount was sealed.

[Example 3] [Production of member (A)] In Example 1, the microchemical device was changed to a member [I-3] (1) having a flow path (4).
3) A member having a decompression chamber (5) [II-3] (14)
Is divided into two members which can be fixed to each other with claws (11, 11 '). The flow path of the member [I-3] (13) is airtight to the outside by the O-ring (12). In the state, the member is connected to the decompression chamber (5) of the member [II-3] (14), and the decompression chamber (5) is 20 mm in width, 15 mm in length, and 1 in depth.
A rectangular shape of 40 μm, and at the inlet (6):
A microchemical device [D-3] having the shape shown in FIG. 2 was produced in the same manner as in Example 1 except that a polystyrene cylinder (15) having an inner diameter of 7 mm and a height of 10 mm was adhered.

[Liquid sending test] Microchemical device [D-
3] is placed with the member (A) side up, that is, with the inlet (6) side up, and colored with methylene blue (Kanto Chemical) using a pipette in the tube (15) of the inlet (6). The distilled water was injected, and the air in the decompression chamber (5) was removed from the exhaust port (8) by an aspirator. The water flowed through the continuous flow path (4) and flowed into the decompression chamber (5).

<Example 4> [Preparation of microchemical device] In Example 1, instead of polystyrene, acrylic resin ("Delpet 670N" manufactured by Asahi Kasei Kogyo Co., Ltd.) was used as the material of the member (A) and the member (B). )), Polycarbonate ("Iupilon S-2000" manufactured by Mitsubishi Engineering-Plastics Corporation), polysulfone ("Udel P-1700" manufactured by Amoco), polyarylate resin ("U-Polymer U-70" manufactured by Unitika Ltd.),
Except for using each transparent hard vinyl chloride resin,
In the same manner as in Example 1, the fine chemical device [D4-
1 to 5].

[Liquid sending test] Microchemical device [D4
-1 to 5], the same test as in Example 1 was performed, and the same result as in Example 1 was obtained.

<Example 5> [Production of microchemical device] In Example 3, instead of polystyrene, acrylic resin ("Delpet 670N" manufactured by Asahi Kasei Kogyo Co., Ltd.) was used as the material of the members (A) and (B). )), Polycarbonate ("Iupilon S-2000" manufactured by Mitsubishi Engineering-Plastics Co., Ltd., polysulfone ("Udel P-1700" manufactured by Amoco)), polyarylate resin ("U-Polymer U-7 manufactured by Unitika Ltd.")
0) and microchemical devices [D5-1-5] were prepared in the same manner as in Example 3 except that a transparent hard vinyl chloride resin was used.

[Liquid sending test] Microchemical device [D5
-1 to 5], the same test as in Example 3 was performed, and the same result as in Example 3 was obtained.

[0058]

The microchemical device of the present invention does not require a liquid feed pump when used for applications such as reaction, analysis, and inspection, so that it is easy to process many devices simultaneously and in parallel. It is possible to improve the efficiency in each application.

[Brief description of the drawings]

FIGS. 1A and 1B are a partial cross-sectional plan view and a front view of a microchemical device manufactured in Example 1 as viewed from a direction perpendicular to the surface of a member (B).

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 Polystyrene board 2 Energy beam curable composition [e1] Cured material layer 3 Energy beam curable composition [e2] Cured material layer 4 Capillary channel 5 Decompression chamber 6 Inlet 7 Check valve 8 Exhaust port 9 Polystyrene Plate 10 Adhesive, energy ray-curable composition [e1] cured product

FIGS. 2A and 2B are a plan view and a front view of the microchemical device manufactured in Example 3 as viewed from a direction perpendicular to the surface of a member (B).

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 Polystyrene board 2 Energy beam curable composition [e1] Cured material layer 3 Energy beam curable composition [e2] Cured material layer 4 Capillary channel 5 Decompression chamber 6 Inlet 7 Check valve 8 Exhaust port 9 Polystyrene Plate 10 Energy ray-curable composition [e1] Cured product, adhesive 11 Hook 11 'Hook 12 O-ring 13 Member (I) 14 Member (II) 15 tube

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) G01N 27/62 G01N 30/60 D 4G075 30/60 31/20 31/20 35/08 A 35/08 C12M 1/00 A // C12M 1/00 G01N 27/26 331E C12N 15/09 C12N 15/00 A F term (reference) 2G042 AA01 CA02 CB03 EA08 HA01 HA03 HA07 2G058 EA03 EA14 EB19 3F060 AA10 GA14 HA00 4B024 AA11 AA29 HA11 4B AA07 AA23 AA27 BB15 BB20 CC01 FA15 4G075 AA13 AA62 CA05 EB21 FB12

Claims (10)

[Claims] (A) between a member (A) and a member (B),
By filling the solid material except for the portion that becomes the flow path, or (b) by bonding another member (B) to the grooved surface of the member (A) having a groove on the surface,
A capillary having a width of 1 to 1000 μm and a depth of 1 to 1000 μm between the member (A) and the member (B) bonded to each other when viewed from a direction perpendicular to the bonding surface of the member (A) and the member (B). A microchemical device in which a flow path of
A microchemical device, characterized by having a decompression chamber in communication with the flow path and capable of decompression inside the microchemical device. 2. The microchemical device according to claim 1, wherein the decompression chamber is provided between the members (A) and (B). 3. The microchemical device according to claim 1, wherein an exhaust port having a check valve is provided in the decompression chamber. 4. The microchemical device according to claim 1, wherein the member (A) and the member (B) are formed of an organic polymer. 5. The member (A) and the member (B) are:
The styrene-based polymer, the (meth) acryl-based polymer, the polycarbonate-based polymer, the polysulfone-based polymer, the polyester-based polymer, and the vinyl chloride-based polymer. Micro chemical device. 6. A member having a width of 1 to 1000 μm and a depth of 1 between a member (A) and a member (B) bonded to each other when viewed from a direction perpendicular to a bonding surface between the member (A) and the member (B). ~ 1
Member having a 000 μm capillary flow path (I)
And a microchemical device comprising a member (II) provided with a decompression chamber, wherein the member (II) is connected to the member (I) so that the decompression chamber of the member (II) communicates with the flow path of the member (I). A microchemical device which is fixed. 7. The microchemical device according to claim 6, wherein the member (I) and the member (II) are detachable. 8. The microchemical device according to claim 6, wherein an exhaust port having a check valve is provided in the decompression chamber. 9. The microchemical device according to claim 6, wherein the member (A) and the member (B) are formed of an organic polymer. 10. The member (A) and the member (B) are a styrene polymer, a (meth) acrylic polymer, a polycarbonate polymer, a polysulfone polymer, a polyester polymer, and a vinyl chloride polymer, respectively. 9. The microchemical device according to claim 6, which is formed of a polymer selected from the group consisting of: