JP2000514300A - ヒトサイトメガロウイルス(cmv)を検出するためのペプチド試薬 - Google Patents
ヒトサイトメガロウイルス(cmv)を検出するためのペプチド試薬Info
- Publication number
- JP2000514300A JP2000514300A JP10505609A JP50560998A JP2000514300A JP 2000514300 A JP2000514300 A JP 2000514300A JP 10505609 A JP10505609 A JP 10505609A JP 50560998 A JP50560998 A JP 50560998A JP 2000514300 A JP2000514300 A JP 2000514300A
- Authority
- JP
- Japan
- Prior art keywords
- peptide
- cmv
- seq
- amino acid
- acid sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
- G01N33/56994—Herpetoviridae, e.g. cytomegalovirus, Epstein-Barr virus
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
- C07K16/081—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from DNA viruses
- C07K16/085—Herpetoviridae, e.g. pseudorabies virus, Epstein-Barr virus
- C07K16/089—Cytomegalovirus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16111—Cytomegalovirus, e.g. human herpesvirus 5
- C12N2710/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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- Molecular Biology (AREA)
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- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
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- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1. サイトメガロウイルスに対する抗体を検出するためのペプチド試薬であっ て、前記試薬がサイトメガロウイルス構造リンタンパク質pp150(UL32 )に由来するペプチドとサイトメガロウイルスタンパク質;pp52(UL44 )、pp28(UL99)、又はgB(UL55)の1つに由来する1又は2以 上のペプチドとを含有していることを特徴とするペプチド試薬。 2. CMV pp150タンパク質に由来する前記ペプチドが配列番号1〜6 に示されているアミノ酸配列の少なくとも一部を含有していることを特徴とする 、請求項1に記載のペプチド試薬。 3. CMV pp150タンパク質に由来する前記ペプチドが配列番号1に示 されているアミノ酸配列の少なくとも一部を含有していることを特徴とする、請 求項2に記載のペプチド試薬。 4. CMV gBタンパク質に由来する前記ペプチドが配列番号7に示されて いるアミノ酸配列の少なくとも一部を含有し ていることを特徴とする、請求項1に記載のペプチド試薬。 5. CMV pp52タンパク質に由来する前記ペプチドが配列番号8に示さ れているアミノ酸配列の少なくとも一部を含有していることを特徴とする、請求 項1に記載のペプチド試薬。 6. CMV pp28タンパク質に由来する前記ペプチドが配列番号9〜10 に示されているアミノ酸配列の少なくとも一部を含有していることを特徴とする 、請求項1に記載のペプチド試薬。 7. CMV pp28タンパク質に由来する前記ペプチドが配列番号10に示 されているアミノ酸配列の少なくとも一部を含有していることを特徴とする、請 求項6に記載のペプチド試薬。 8. 配列番号1に示されているアミノ酸配列を有するペプチドと、配列番号7 に示されているアミノ酸配列を有するペプチドと、さらに配列番号10に示され ているアミノ酸配列を有するペプチドとを含有している、請求項1に記載のペプ チド試薬。 9. 配列番号1に示されているアミノ酸配列を有するペプチドと配列番号8に 示されているアミノ酸配列を有するペプチドとを含有している、請求項1に記載 のペプチド試薬。 10. 配列番号1〜10に示されているアミノ酸配列を有するペプチドのエピ トープに結合するモノクローナル抗体。 11. CMV pp150−タンパク質のエピトープに結合するモノクローナ ル抗体であって、番号No.96071123を付けて欧州動物細胞培養コレク ション(European collection of Animal Ce ll Cultures(ECACC),CAMR(Centerfor Ap plied Microbiology & Research:応用微生物学 研究センター),Salisbury(英国)に寄託されているハイブリドーマ 細胞系によって産生したモノクローナル抗体CMV.OT3Cによって該エピト ープが認識されることを特徴とするモノクローナル抗体。 12. 請求項10〜11のいずれかに記載のモノクローナル抗体を産生するこ とができる不死化細胞系。 13. 請求項10〜11のいずれかに記載の抗体を含む免疫化学的試薬。 14. 試料中に含まれるサイトメガロウイルスの検出方法であって、前記試料 を請求項13に記載の免疫化学的試薬に接触させ、その後に形成された免疫複合 体の存在を検出することか ら成る検出方法。 15. 被験液中に含まれるサイトメガロウイルスに対する抗体の検出方法であ って、被験液を請求項1〜9のいずれかに記載のペプチド試薬と接触させ、さら に被験液中で形成された免疫複合体類の存在を検出することを特徴とする検出方 法。 16. 被験液中に含まれるサイトメガロウイルスの検出方法であって、被験液 を請求項1〜9のいずれかに記載のペプチド試薬と接触させ、それにサイトメガ ロウイルスに対する抗体を接触させ、その後に形成された免疫複合体の存在を検 出することを特徴とする検出方法。 17. 請求項14〜16のいずれかに記載の方法を実行するための試験キット 。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP96201972 | 1996-07-12 | ||
EP96201972.5 | 1996-07-12 | ||
PCT/EP1997/003717 WO1998002746A1 (en) | 1996-07-12 | 1997-07-09 | Peptide reagent for the detection of human cytomegalovirus (cmv) |
Publications (2)
Publication Number | Publication Date |
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JP2000514300A true JP2000514300A (ja) | 2000-10-31 |
JP4218985B2 JP4218985B2 (ja) | 2009-02-04 |
Family
ID=8224180
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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JP50560998A Expired - Lifetime JP4218985B2 (ja) | 1996-07-12 | 1997-07-09 | ヒトサイトメガロウイルス(cmv)を検出するためのペプチド試薬 |
Country Status (12)
Country | Link |
---|---|
US (1) | US6936251B2 (ja) |
EP (1) | EP0960336B1 (ja) |
JP (1) | JP4218985B2 (ja) |
KR (1) | KR100624517B1 (ja) |
AT (1) | ATE225942T1 (ja) |
AU (1) | AU735981B2 (ja) |
DE (1) | DE69716305T2 (ja) |
DK (1) | DK0960336T3 (ja) |
ES (1) | ES2187796T3 (ja) |
ID (1) | ID19063A (ja) |
WO (1) | WO1998002746A1 (ja) |
ZA (1) | ZA976196B (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012005238A1 (ja) * | 2010-07-07 | 2012-01-12 | 富士レビオ株式会社 | ヒトサイトメガロウイルス感染の検出方法 |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
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DE19919121B4 (de) * | 1999-04-27 | 2012-08-02 | F. Hoffmann-La Roche Ag | Multimeres CMV-Antigen |
AU2002214624A1 (en) * | 2000-10-20 | 2002-05-06 | City Of Hope | Immunoreactive peptide ctl epitopes of human cytomegalovirus pp150 |
AUPR593101A0 (en) * | 2001-06-26 | 2001-07-19 | Council Of The Queensland Institute Of Medical Research, The | Cytomegalovirus t cell epitopes |
ITMI20012160A1 (it) * | 2001-10-18 | 2003-04-18 | Edoardo Marchisio | Sistema immunoenzimatico per la caratterizzazione antigenica dell'infezione attiva da cytomegalovirus e cmv confirmation test |
GB0700133D0 (en) | 2007-01-04 | 2007-02-14 | Humabs Llc | Human cytomegalovirus neutralising antibodies and use thereof |
US7947274B2 (en) | 2007-01-04 | 2011-05-24 | Humabs, LLC. | Human cytomegalovirus neutralising antibodies and use thereof |
CN101605808A (zh) * | 2007-02-07 | 2009-12-16 | 希格马托制药工业公司 | 人巨细胞病毒(hcmv)的重组抗原 |
MX343490B (es) | 2008-07-16 | 2016-11-08 | Inst For Res In Biomedicine | Anticuerpos neutralizantes de citomegalovirus humano y uso de los mismos. |
US20120093848A1 (en) * | 2009-06-26 | 2012-04-19 | Vectorite Biomedica Inc. | Immunogenic composition comprising peptides derived from cytomegalovirus and the use thereof |
NZ591514A (en) * | 2011-03-03 | 2013-11-29 | Kode Biotech Ltd | Assay method |
US10611800B2 (en) | 2016-03-11 | 2020-04-07 | Pfizer Inc. | Human cytomegalovirus gB polypeptide |
US11629172B2 (en) | 2018-12-21 | 2023-04-18 | Pfizer Inc. | Human cytomegalovirus gB polypeptide |
TWI810589B (zh) | 2020-06-21 | 2023-08-01 | 美商輝瑞股份有限公司 | 人巨細胞病毒糖蛋白B(gB)多肽 |
Family Cites Families (10)
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WO1987003602A1 (en) * | 1985-12-06 | 1987-06-18 | Teijin Limited | Anticytomegaloviral human monoclonal antibody and process for its preparation |
CA1335429C (en) * | 1986-03-07 | 1995-05-02 | Geoffrey L. Smith | Processes for the production of hcmv glycoproteins, antibodies thereto and hcmv vaccines, and recombinant vectors therefor |
DE3619902A1 (de) * | 1986-06-13 | 1988-03-03 | Biotechnolog Forschung Gmbh | Cmv-dna-strukturen, expressionsvektor dafuer, cmv-proteinstrukturen und deren verwendung |
DE58909550D1 (de) * | 1988-02-24 | 1996-02-15 | Behringwerke Ag | Strukturelles Phosphoprotein (pp28) des menschlichen Cytomegalovirus (HCMV), seine Herstellung und Verwendung |
GB9014652D0 (en) * | 1990-07-02 | 1990-08-22 | Wellcome Found | Antigen |
US5744298A (en) * | 1991-08-29 | 1998-04-28 | Behringwerke Aktiengesellschaft | DNA sequences which encode immunoreactive HCMV-specific peptides |
DE4128684A1 (de) | 1991-08-29 | 1993-03-04 | Behringwerke Ag | Hcmv-spezifische peptide, mittel dazu und ihre verwendung |
IT1266740B1 (it) * | 1994-07-01 | 1997-01-14 | Maria Paola Landini | Materiale proteico ricombinante legante anticorpi contro il citomegalovirus umano, reagenti diagnostici derivati da tale |
EP0702083A3 (de) * | 1994-07-26 | 1997-01-15 | Biotest Ag | Polypeptide und Fusionsproteine bestehend aus dem UL 57 Leserahmen bzw. dem C-terminalen Bereich des Tegumentproteins pp150 aus HCMV, entsprechende Oligonucleotide und Nachweis reagentien |
DE4426453C1 (de) * | 1994-07-26 | 1995-11-02 | Biotest Ag | Rekombinant hergestellte Fusionsproteine des Cytomegalievirus und diese enthaltende diagnostische Testkits |
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1997
- 1997-07-09 AU AU36231/97A patent/AU735981B2/en not_active Expired
- 1997-07-09 EP EP97932819A patent/EP0960336B1/en not_active Expired - Lifetime
- 1997-07-09 DK DK97932819T patent/DK0960336T3/da active
- 1997-07-09 AT AT97932819T patent/ATE225942T1/de not_active IP Right Cessation
- 1997-07-09 ES ES97932819T patent/ES2187796T3/es not_active Expired - Lifetime
- 1997-07-09 KR KR1019997000160A patent/KR100624517B1/ko not_active IP Right Cessation
- 1997-07-09 JP JP50560998A patent/JP4218985B2/ja not_active Expired - Lifetime
- 1997-07-09 DE DE69716305T patent/DE69716305T2/de not_active Expired - Lifetime
- 1997-07-09 WO PCT/EP1997/003717 patent/WO1998002746A1/en active IP Right Grant
- 1997-07-11 ZA ZA976196A patent/ZA976196B/xx unknown
- 1997-07-14 ID IDP972439A patent/ID19063A/id unknown
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2002
- 2002-11-05 US US10/288,220 patent/US6936251B2/en not_active Expired - Lifetime
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012005238A1 (ja) * | 2010-07-07 | 2012-01-12 | 富士レビオ株式会社 | ヒトサイトメガロウイルス感染の検出方法 |
JP2012018051A (ja) * | 2010-07-07 | 2012-01-26 | Fujirebio Inc | ヒトサイトメガロウイルス感染の検出方法 |
Also Published As
Publication number | Publication date |
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JP4218985B2 (ja) | 2009-02-04 |
ZA976196B (en) | 1998-09-01 |
KR20000023701A (ko) | 2000-04-25 |
ES2187796T3 (es) | 2003-06-16 |
AU3623197A (en) | 1998-02-09 |
AU735981B2 (en) | 2001-07-19 |
KR100624517B1 (ko) | 2006-09-18 |
US6936251B2 (en) | 2005-08-30 |
WO1998002746A1 (en) | 1998-01-22 |
EP0960336A1 (en) | 1999-12-01 |
DE69716305D1 (de) | 2002-11-14 |
ID19063A (id) | 1998-06-11 |
EP0960336B1 (en) | 2002-10-09 |
DE69716305T2 (de) | 2003-06-26 |
DK0960336T3 (da) | 2003-02-10 |
US20030119039A1 (en) | 2003-06-26 |
ATE225942T1 (de) | 2002-10-15 |
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