JP2000500967A - 組織特異的および標的rna特異的リボザイム - Google Patents
組織特異的および標的rna特異的リボザイムInfo
- Publication number
- JP2000500967A JP2000500967A JP9518381A JP51838197A JP2000500967A JP 2000500967 A JP2000500967 A JP 2000500967A JP 9518381 A JP9518381 A JP 9518381A JP 51838197 A JP51838197 A JP 51838197A JP 2000500967 A JP2000500967 A JP 2000500967A
- Authority
- JP
- Japan
- Prior art keywords
- ribozyme
- nucleic acid
- sequence
- specific
- rna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000053642 Catalytic RNA Human genes 0.000 title claims abstract description 210
- 108090000994 Catalytic RNA Proteins 0.000 title claims abstract description 210
- 108091092562 ribozyme Proteins 0.000 title claims abstract description 210
- 108091032973 (ribonucleotides)n+m Proteins 0.000 title claims abstract description 74
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 67
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 66
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 66
- 238000000034 method Methods 0.000 claims abstract description 34
- 230000003197 catalytic effect Effects 0.000 claims abstract description 26
- 230000009870 specific binding Effects 0.000 claims abstract description 15
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 14
- 201000010099 disease Diseases 0.000 claims abstract description 13
- 210000001519 tissue Anatomy 0.000 claims description 59
- 238000003776 cleavage reaction Methods 0.000 claims description 43
- 230000007017 scission Effects 0.000 claims description 43
- 239000013598 vector Substances 0.000 claims description 32
- 230000014509 gene expression Effects 0.000 claims description 28
- 210000002307 prostate Anatomy 0.000 claims description 21
- 230000027455 binding Effects 0.000 claims description 20
- 239000002773 nucleotide Substances 0.000 claims description 18
- 125000003729 nucleotide group Chemical group 0.000 claims description 18
- 206010060862 Prostate cancer Diseases 0.000 claims description 16
- 208000000236 Prostatic Neoplasms Diseases 0.000 claims description 16
- 241001465754 Metazoa Species 0.000 claims description 14
- 108090000623 proteins and genes Proteins 0.000 claims description 14
- 230000009261 transgenic effect Effects 0.000 claims description 12
- 108010042121 probasin Proteins 0.000 claims description 9
- 230000002062 proliferating effect Effects 0.000 claims description 8
- 230000004570 RNA-binding Effects 0.000 claims description 7
- 150000001875 compounds Chemical class 0.000 claims description 7
- 208000015181 infectious disease Diseases 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 238000011144 upstream manufacturing Methods 0.000 claims description 6
- 230000004663 cell proliferation Effects 0.000 claims description 5
- 239000012678 infectious agent Substances 0.000 claims description 5
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 4
- 210000004602 germ cell Anatomy 0.000 claims description 4
- 108020004418 ribosomal RNA Proteins 0.000 claims description 4
- 210000001082 somatic cell Anatomy 0.000 claims description 4
- 238000012216 screening Methods 0.000 claims description 3
- 238000010186 staining Methods 0.000 claims 1
- 206010028980 Neoplasm Diseases 0.000 abstract description 15
- 208000036142 Viral infection Diseases 0.000 abstract description 8
- 230000009385 viral infection Effects 0.000 abstract description 8
- 210000004027 cell Anatomy 0.000 description 27
- 238000012384 transportation and delivery Methods 0.000 description 13
- 108020004414 DNA Proteins 0.000 description 11
- 230000000694 effects Effects 0.000 description 11
- 239000002502 liposome Substances 0.000 description 11
- 101150076840 PolI gene Proteins 0.000 description 9
- 238000001727 in vivo Methods 0.000 description 9
- 102000004169 proteins and genes Human genes 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 201000011510 cancer Diseases 0.000 description 8
- 238000010367 cloning Methods 0.000 description 8
- 238000001415 gene therapy Methods 0.000 description 8
- 230000008685 targeting Effects 0.000 description 8
- 230000000692 anti-sense effect Effects 0.000 description 7
- 210000004185 liver Anatomy 0.000 description 7
- 241000699670 Mus sp. Species 0.000 description 6
- 230000030833 cell death Effects 0.000 description 6
- 210000000981 epithelium Anatomy 0.000 description 6
- 239000012634 fragment Substances 0.000 description 6
- 238000000338 in vitro Methods 0.000 description 6
- 238000002347 injection Methods 0.000 description 6
- 239000007924 injection Substances 0.000 description 6
- 238000013518 transcription Methods 0.000 description 6
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 5
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 5
- 241000699660 Mus musculus Species 0.000 description 5
- 238000013459 approach Methods 0.000 description 5
- 239000005090 green fluorescent protein Substances 0.000 description 5
- 230000003902 lesion Effects 0.000 description 5
- 210000004072 lung Anatomy 0.000 description 5
- 201000004792 malaria Diseases 0.000 description 5
- 108020004999 messenger RNA Proteins 0.000 description 5
- 230000002441 reversible effect Effects 0.000 description 5
- 230000035897 transcription Effects 0.000 description 5
- 238000011830 transgenic mouse model Methods 0.000 description 5
- 102100027211 Albumin Human genes 0.000 description 4
- 108010088751 Albumins Proteins 0.000 description 4
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 4
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 4
- 108091034117 Oligonucleotide Proteins 0.000 description 4
- 208000030852 Parasitic disease Diseases 0.000 description 4
- 108010072866 Prostate-Specific Antigen Proteins 0.000 description 4
- 102100038358 Prostate-specific antigen Human genes 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 230000000295 complement effect Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 208000006454 hepatitis Diseases 0.000 description 4
- 231100000283 hepatitis Toxicity 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000011200 topical administration Methods 0.000 description 4
- 238000001890 transfection Methods 0.000 description 4
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 3
- 208000035143 Bacterial infection Diseases 0.000 description 3
- 101000909256 Caldicellulosiruptor bescii (strain ATCC BAA-1888 / DSM 6725 / Z-1320) DNA polymerase I Proteins 0.000 description 3
- 241000700721 Hepatitis B virus Species 0.000 description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
- 101000902592 Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) DNA polymerase Proteins 0.000 description 3
- 102000017143 RNA Polymerase I Human genes 0.000 description 3
- 108010013845 RNA Polymerase I Proteins 0.000 description 3
- 241000251131 Sphyrna Species 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 150000007513 acids Chemical class 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 208000022362 bacterial infectious disease Diseases 0.000 description 3
- 239000003054 catalyst Substances 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 210000003743 erythrocyte Anatomy 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 239000002777 nucleoside Substances 0.000 description 3
- 230000010076 replication Effects 0.000 description 3
- 238000001356 surgical procedure Methods 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 108091026890 Coding region Proteins 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 102100033183 Epithelial membrane protein 1 Human genes 0.000 description 2
- XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 description 2
- 102100034343 Integrase Human genes 0.000 description 2
- WTDRDQBEARUVNC-LURJTMIESA-N L-DOPA Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-LURJTMIESA-N 0.000 description 2
- 108700020796 Oncogene Proteins 0.000 description 2
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 2
- 108020000999 Viral RNA Proteins 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- 230000000890 antigenic effect Effects 0.000 description 2
- 210000002376 aorta thoracic Anatomy 0.000 description 2
- 210000000481 breast Anatomy 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 230000021164 cell adhesion Effects 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 210000000805 cytoplasm Anatomy 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000002716 delivery method Methods 0.000 description 2
- 238000010494 dissociation reaction Methods 0.000 description 2
- 230000005593 dissociations Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 108010008594 epithelial membrane protein-1 Proteins 0.000 description 2
- 210000002950 fibroblast Anatomy 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 238000001990 intravenous administration Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 125000003835 nucleoside group Chemical group 0.000 description 2
- 210000004940 nucleus Anatomy 0.000 description 2
- 244000045947 parasite Species 0.000 description 2
- 230000036281 parasite infection Effects 0.000 description 2
- 238000007911 parenteral administration Methods 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 210000002460 smooth muscle Anatomy 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 230000009897 systematic effect Effects 0.000 description 2
- 230000014621 translational initiation Effects 0.000 description 2
- 201000008827 tuberculosis Diseases 0.000 description 2
- WALUVDCNGPQPOD-UHFFFAOYSA-M 2,3-di(tetradecoxy)propyl-(2-hydroxyethyl)-dimethylazanium;bromide Chemical compound [Br-].CCCCCCCCCCCCCCOCC(C[N+](C)(C)CCO)OCCCCCCCCCCCCCC WALUVDCNGPQPOD-UHFFFAOYSA-M 0.000 description 1
- 108020000948 Antisense Oligonucleotides Proteins 0.000 description 1
- 101100290380 Caenorhabditis elegans cel-1 gene Proteins 0.000 description 1
- 206010008263 Cervical dysplasia Diseases 0.000 description 1
- 102100031673 Corneodesmosin Human genes 0.000 description 1
- 101710139375 Corneodesmosin Proteins 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 101710177611 DNA polymerase II large subunit Proteins 0.000 description 1
- 101710184669 DNA polymerase II small subunit Proteins 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 206010058314 Dysplasia Diseases 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 102000030914 Fatty Acid-Binding Human genes 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 101001091385 Homo sapiens Kallikrein-6 Proteins 0.000 description 1
- 101001128694 Homo sapiens Neuroendocrine convertase 1 Proteins 0.000 description 1
- 101100156155 Human papillomavirus type 16 E7 gene Proteins 0.000 description 1
- 101000954493 Human papillomavirus type 16 Protein E6 Proteins 0.000 description 1
- 102100034866 Kallikrein-6 Human genes 0.000 description 1
- WTDRDQBEARUVNC-UHFFFAOYSA-N L-Dopa Natural products OC(=O)C(N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-UHFFFAOYSA-N 0.000 description 1
- 102000006835 Lamins Human genes 0.000 description 1
- 108010047294 Lamins Proteins 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241000187479 Mycobacterium tuberculosis Species 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 102000008297 Nuclear Matrix-Associated Proteins Human genes 0.000 description 1
- 108010035916 Nuclear Matrix-Associated Proteins Proteins 0.000 description 1
- 102000043276 Oncogene Human genes 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 206010033724 Papilloma viral infections Diseases 0.000 description 1
- 241000223960 Plasmodium falciparum Species 0.000 description 1
- 206010036774 Proctitis Diseases 0.000 description 1
- 102000052575 Proto-Oncogene Human genes 0.000 description 1
- 108700020978 Proto-Oncogene Proteins 0.000 description 1
- 102000007568 Proto-Oncogene Proteins c-fos Human genes 0.000 description 1
- 108010071563 Proto-Oncogene Proteins c-fos Proteins 0.000 description 1
- 101710151532 Replicating protein Proteins 0.000 description 1
- 241000231739 Rutilus rutilus Species 0.000 description 1
- 108091081021 Sense strand Proteins 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 208000000453 Skin Neoplasms Diseases 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 101100388071 Thermococcus sp. (strain GE8) pol gene Proteins 0.000 description 1
- 208000006374 Uterine Cervicitis Diseases 0.000 description 1
- 101710087237 Whey acidic protein Proteins 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 210000004100 adrenal gland Anatomy 0.000 description 1
- 230000033115 angiogenesis Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000000074 antisense oligonucleotide Substances 0.000 description 1
- 238000012230 antisense oligonucleotides Methods 0.000 description 1
- 239000003443 antiviral agent Substances 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 230000037429 base substitution Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 108091092328 cellular RNA Proteins 0.000 description 1
- 230000004745 cervical carcinogenesis Effects 0.000 description 1
- 206010008323 cervicitis Diseases 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 201000003146 cystitis Diseases 0.000 description 1
- 108091092330 cytoplasmic RNA Proteins 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- UMGXUWVIJIQANV-UHFFFAOYSA-M didecyl(dimethyl)azanium;bromide Chemical compound [Br-].CCCCCCCCCC[N+](C)(C)CCCCCCCCCC UMGXUWVIJIQANV-UHFFFAOYSA-M 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 108091022862 fatty acid binding Proteins 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 210000004392 genitalia Anatomy 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000002440 hepatic effect Effects 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000010255 intramuscular injection Methods 0.000 description 1
- 239000007927 intramuscular injection Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000005053 lamin Anatomy 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000006193 liquid solution Substances 0.000 description 1
- 239000006194 liquid suspension Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 230000001613 neoplastic effect Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 210000000299 nuclear matrix Anatomy 0.000 description 1
- 150000003833 nucleoside derivatives Chemical class 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 238000009595 pap smear Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000007542 postnatal development Effects 0.000 description 1
- 210000005267 prostate cell Anatomy 0.000 description 1
- 208000023958 prostate neoplasm Diseases 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 201000000849 skin cancer Diseases 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000012385 systemic delivery Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 108010039429 vascin Proteins 0.000 description 1
- 210000005166 vasculature Anatomy 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- 230000029812 viral genome replication Effects 0.000 description 1
- 230000017613 viral reproduction Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/03—Animal model, e.g. for test or diseases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/11—Antisense
- C12N2310/111—Antisense spanning the whole gene, or a large part of it
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
- C12N2310/121—Hammerhead
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
- C12N2310/127—DNAzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2799/00—Uses of viruses
- C12N2799/02—Uses of viruses as vector
- C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Animal Behavior & Ethology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Virology (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Tropical Medicine & Parasitology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.5’自己触媒的切断リボザイム配列、標的RNA特異的結合部位を含む触媒リ ボザイム、および3’自己触媒的切断リボザイム配列をコードする配列から上流 の標的特異的プロモーター結合部位を含む、核酸。 2.前記標的特異的プロモーター結合部位が、プロバシンプロモーターの結合部 位である、請求項1に記載の核酸。 3.前記5'自己触媒的切断リボザイムをコードする核酸が、配列番号1として 配列表に示されるヌクレオチド9〜62の配列を有する。請求項1に記載の核酸。 4.前記3'自己触媒的切断リボザイムをコードする核酸が、配列番号1として 配列表に示されるヌクレオチド119〜172の配列を有する。請求項1に記載の核酸 。 5.前記核酸が、リボソームRNA(ポリメラーゼI(A))に独特のRNA配列に結合す る標的RNA結合部位を含む触媒リボザイムをコードする、請求項1に記載の核酸 。 6.前記核酸が、配列番号1として配列表に示されるヌクレオチド72〜113の配 列を有する、請求項5に記載の核酸。 7.配列番号1として配列表に示される配列のヌクレオチドから本質的になる、 請求項1に記載の核酸。 8.配列番号1として配列表に示される配列を有する、請求項1に記載の核酸。 9.ベクター中の、請求項1に記載の核酸。 10.ベクター中の、請求項2に記載の核酸。 11.ベクター中の、請求項3に記載の核酸。 12.ベクター中の、請求項4に記載の核酸。 13.ベクター中の、請求項5に記載の核酸。 14.ベクター中の、請求項6に記載の核酸。 15.ベクター中の、請求項7に記載の核酸。 16.ベクター中の、請求項8に記載の核酸。 17.生殖細胞または体細胞中に、請求項1に記載の核酸を含むトランスジェニ ック非ヒト動物であって、ここで、該動物は、5’自己触媒的切断リボザイム配 列、標的RNA特異的結合部位を含む触媒リボザイム、および3’自己触媒的切断 リボザイム配列を含むリボザイムを発現する、トランスジェニック非ヒト動物。 18.生殖細胞または体細胞中に、請求項7に記載の核酸を含むトランスジェニ ック非ヒト動物であって、ここで、該動物は、5’自己触媒的切断リボザイム配 列、標的RNA特異的結合部位を含む触媒リボザイム、および3’自己触媒的切断 リボザイム配列を含むリボザイムを発現する、トランスジェニック非ヒト動物。 19.生殖細胞または体細胞中に、請求項8に記載の核酸を含むトランスジェニ ック非ヒト動物であって、ここで、該動物は、5’自己触媒的切断リボザイム配 列、標的RNA特異的結合部位を含む触媒リボザイム、および3'自己触媒的切断リ ボザイム配列を含むリボザイムを発現する、トランスジェニック非ヒト動物。 20.前記動物が、標的RNAに関連する表現型を発現しない、請求項17に記載 のトランスジェニック非ヒト動物。 21.請求項20に記載の動物に、前記標的RNAに関連する表現型を発現させる 能力について化合物をスクリーニングする方法であって、該化合物を該動物に投 与する工程、および該表現型の発現を引き起こす能力を評価する工程を包含する 、方法。 22.特定の組織の増殖性疾患を有する被験体を、該組織の細胞増殖を阻害する ことによって処置する方法であって、請求項5に記載の核酸を該被験体に投与す る工程を包含し、ここで、標的特異的プロモーター結合配列は、疾患組織に特異 的であり、これにより該核酸にコードされるリボザイムが発現され、該組織にお けるリボソームRNA産生が阻害され、細胞増殖が阻害され、そして該増殖性疾患 が処置される、方法。 23.前立腺癌を有する被験体を処置する方法であって、請求項7に記載の核酸 を該被験体に投与する工程を包含し、これにより、該核酸にコードされるリボザ イムが該前立腺において発現され、そして該前立腺癌が処置される、方法。 24.被験体の感染を処置する方法であって、請求項1に記載の核酸を、被験体 に投与する工程を包含し、ここで、コードされる標的RNA特異的結合部位は、感 染因子に独特のRNAに特異的であり、これにより核酸にコードされるリボザイム が発現され、そして該感染因子が殺傷される、方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/554,369 | 1995-11-08 | ||
US08/554,369 US5824519A (en) | 1995-11-08 | 1995-11-08 | Tissue-specific and target RNA-specific ribozymes |
PCT/US1996/018088 WO1997017433A1 (en) | 1995-11-08 | 1996-11-08 | Tissue-specific and target rna-specific ribozymes |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006283150A Division JP2007084550A (ja) | 1995-11-08 | 2006-10-17 | 組織特異的および標的rna特異的リボザイム |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2000500967A true JP2000500967A (ja) | 2000-02-02 |
JP4033902B2 JP4033902B2 (ja) | 2008-01-16 |
Family
ID=24213065
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP51838197A Expired - Fee Related JP4033902B2 (ja) | 1995-11-08 | 1996-11-08 | 組織特異的および標的rna特異的リボザイム |
JP2006283150A Pending JP2007084550A (ja) | 1995-11-08 | 2006-10-17 | 組織特異的および標的rna特異的リボザイム |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006283150A Pending JP2007084550A (ja) | 1995-11-08 | 2006-10-17 | 組織特異的および標的rna特異的リボザイム |
Country Status (9)
Country | Link |
---|---|
US (1) | US5824519A (ja) |
EP (2) | EP0866852B1 (ja) |
JP (2) | JP4033902B2 (ja) |
CN (1) | CN1207769A (ja) |
AT (1) | ATE294854T1 (ja) |
AU (1) | AU728732B2 (ja) |
DE (1) | DE69634698T2 (ja) |
IL (1) | IL124383A0 (ja) |
WO (1) | WO1997017433A1 (ja) |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998024925A1 (en) * | 1995-11-08 | 1998-06-11 | Medical University Of South Carolina | Tissue specific and target rna-specific ribozymes as antimicrobial therapeutics against microbial pathogens |
US20030125280A1 (en) * | 1996-12-03 | 2003-07-03 | Medical University Of South Carolina | Tissue specific and target RNA-specific ribozymes as antimicrobial therapeutics against microbial pathogens |
ES2233017T3 (es) | 1998-01-15 | 2005-06-01 | King's College London | Acidos nucleicos ribozimales que cortan ccr5 o cxcr4. |
EP1088086A4 (en) * | 1998-06-24 | 2003-01-08 | Musc Found For Res Dev | RIBOZYMES SPECIFIC TO TISSUE AND TARGET RNA |
AU2004201285B2 (en) * | 1998-06-24 | 2007-07-05 | Musc Foundation For Research Development | Tissue-specific and target RNA-specific ribozymes |
US6242589B1 (en) * | 1998-07-14 | 2001-06-05 | Isis Pharmaceuticals, Inc. | Phosphorothioate oligonucleotides having modified internucleoside linkages |
DE60027135T2 (de) * | 1999-01-21 | 2007-01-11 | Metamorphix, Inc. | Inhibitoren für wachstumdifferenzierungsfaktor und ihre anwendungen |
DE19956568A1 (de) * | 1999-01-30 | 2000-08-17 | Roland Kreutzer | Verfahren und Medikament zur Hemmung der Expression eines vorgegebenen Gens |
US6271359B1 (en) | 1999-04-14 | 2001-08-07 | Musc Foundation For Research Development | Tissue-specific and pathogen-specific toxic agents and ribozymes |
WO2000061804A1 (en) * | 1999-04-14 | 2000-10-19 | Musc Foundation For Research Development | Tissue-specific and pathogen-specific toxic agents and ribozymes |
US7829693B2 (en) * | 1999-11-24 | 2010-11-09 | Alnylam Pharmaceuticals, Inc. | Compositions and methods for inhibiting expression of a target gene |
EP1702983A3 (en) | 2000-04-13 | 2007-01-10 | Medical University of South Carolina | Tissue-specific and pathogen-specific toxic agents, ribozymes, DNAzymes and antisense oligonucleotides and methods of use thereof |
US20040209263A1 (en) * | 2000-12-07 | 2004-10-21 | Clawson Gary A. | Selection of catalytic nucleic acids targeted to infectious agents |
US8546143B2 (en) | 2001-01-09 | 2013-10-01 | Alnylam Pharmaceuticals, Inc. | Compositions and methods for inhibiting expression of a target gene |
JP2005537028A (ja) * | 2002-06-26 | 2005-12-08 | ザ ペン ステート リサーチ ファウンデーション | ヒト乳頭腫ウイルス感染症を治療する方法及び材料 |
CA2516425A1 (en) * | 2003-02-21 | 2005-02-24 | The Penn State Research Foundation | Rna interference compositions and methods |
US7892563B2 (en) * | 2003-05-20 | 2011-02-22 | Wyeth Holdings Corporation | Methods for treatment of severe acute respiratory syndrome (SARS) |
US7267960B2 (en) | 2003-07-25 | 2007-09-11 | Amgen Inc. | Antagonists and agonists of LDCAM and methods of use |
GB0522578D0 (en) * | 2005-11-04 | 2005-12-14 | King S College London | Ribozymal nucleic acid |
US8129334B2 (en) | 2006-03-31 | 2012-03-06 | The Regents Of The University Of California | Methods and compositions for treating neurodegenerative disorders and Alzheimer'S disease and improving normal memory |
EP2671891A3 (en) | 2008-06-27 | 2014-03-05 | Amgen Inc. | Ang-2 inhibition to treat multiple sclerosis |
US9186370B2 (en) | 2010-03-19 | 2015-11-17 | University Of South Alabama | Methods and compositions for the treatment of cancer |
WO2012135696A2 (en) | 2011-04-01 | 2012-10-04 | University Of South Alabama | Methods and compositions for the diagnosis, classification, and treatment of cancer |
WO2013103401A1 (en) | 2012-01-06 | 2013-07-11 | University Of South Alabama | Methods and compositions for the treatment of cancer |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MC2115A1 (fr) * | 1987-12-15 | 1991-07-05 | Gene Shears Pty Ltd | Ribozynes |
DE3933384A1 (de) * | 1989-10-06 | 1991-04-18 | Hoechst Ag | Multifunktionelle rna mit selbstprozessierungsaktivitaet, ihre herstellung und ihre verwendung |
JP2507895B2 (ja) * | 1989-12-19 | 1996-06-19 | 工業技術院長 | リボザイムの新規合成系 |
JP2580512B2 (ja) * | 1990-11-02 | 1997-02-12 | 工業技術院長 | リボザイムを用いた新規rna転写システム |
US5500357A (en) * | 1990-11-02 | 1996-03-19 | Agency Of Industrial Science & Technology, Ministry Of International Trade & Industry | RNA transcription system using novel ribozyme |
WO1992010590A1 (en) * | 1990-12-10 | 1992-06-25 | Gilead Sciences, Inc. | Inhibition of transcription by formation of triple helixes |
GB9216851D0 (en) * | 1992-08-07 | 1992-09-23 | Univ Manitoba | Dna sequences of rat probasin gene |
-
1995
- 1995-11-08 US US08/554,369 patent/US5824519A/en not_active Expired - Fee Related
-
1996
- 1996-11-08 JP JP51838197A patent/JP4033902B2/ja not_active Expired - Fee Related
- 1996-11-08 AT AT96940377T patent/ATE294854T1/de not_active IP Right Cessation
- 1996-11-08 CN CN96198727A patent/CN1207769A/zh active Pending
- 1996-11-08 IL IL12438396A patent/IL124383A0/xx unknown
- 1996-11-08 EP EP96940377A patent/EP0866852B1/en not_active Expired - Lifetime
- 1996-11-08 DE DE69634698T patent/DE69634698T2/de not_active Expired - Fee Related
- 1996-11-08 WO PCT/US1996/018088 patent/WO1997017433A1/en active IP Right Grant
- 1996-11-08 EP EP05009695A patent/EP1561815A3/en not_active Withdrawn
- 1996-11-08 AU AU77272/96A patent/AU728732B2/en not_active Ceased
-
2006
- 2006-10-17 JP JP2006283150A patent/JP2007084550A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
CN1207769A (zh) | 1999-02-10 |
ATE294854T1 (de) | 2005-05-15 |
JP2007084550A (ja) | 2007-04-05 |
EP1561815A2 (en) | 2005-08-10 |
DE69634698D1 (de) | 2005-06-09 |
EP0866852A1 (en) | 1998-09-30 |
WO1997017433A1 (en) | 1997-05-15 |
IL124383A0 (en) | 1998-12-06 |
AU7727296A (en) | 1997-05-29 |
JP4033902B2 (ja) | 2008-01-16 |
US5824519A (en) | 1998-10-20 |
EP1561815A3 (en) | 2007-11-07 |
AU728732B2 (en) | 2001-01-18 |
DE69634698T2 (de) | 2006-01-19 |
EP0866852B1 (en) | 2005-05-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP4033902B2 (ja) | 組織特異的および標的rna特異的リボザイム | |
JP6959293B2 (ja) | B型肝炎ウイルスの遺伝子発現を阻害するための組成物および方法 | |
AU2006236453B2 (en) | Delivery of siRNA by neutral lipid compositions | |
US6924096B2 (en) | Delivery construct for antisense nucleic acids and methods of use | |
JPH08502950A (ja) | 癌増殖を抑制する方法及び試薬 | |
US20100286241A1 (en) | Compositions comprising k-ras sirna and methods of use | |
JP2008535504A (ja) | 癌を治療するためのガレクチン‐1を標的とするRNAiに基づく手法の使用 | |
JPH10507067A (ja) | テロメラーゼのrna成分 | |
WO2010006239A2 (en) | Regulation of apoptosis by neural specific splice variants of ig20 | |
KR20110086815A (ko) | 텔로머라제 억제제 및 그의 사용 방법 | |
KR20010042848A (ko) | 인슐린유사 성장인자 ⅱ의 안티센스 올리고뉴클레오타이드서열 및 이를 이용한 세포성장 조절방법 | |
Ho et al. | Antisense oligonucleotides selectively regulate aspartyl β-hydroxylase and its truncated protein isoform in vitro but distribute poorly into A549 tumors in vivo | |
WO2009003211A1 (en) | Treatment of rheumatoid arthritis | |
JP2005510204A (ja) | ヘアレス蛋白質発現を阻害するための核酸及びその使用方法 | |
US20100280097A1 (en) | Compositions comprising hif-1 alpha sirna and methods of use thereof | |
CA2236998C (en) | Tissue-specific and target rna-specific ribozymes | |
WO2009143277A2 (en) | Compositions comprising hscn9a sirna and methods of use thereof | |
US20100273858A1 (en) | Compositions comprising stat5 sirna and methods of use thereof | |
WO2006092714A2 (en) | Inhibition of spag9 expression with sirnas |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20060718 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20061017 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20070206 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20070416 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20071002 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20071024 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20101102 Year of fee payment: 3 |
|
R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
LAPS | Cancellation because of no payment of annual fees |