JP2000500961A - 新規のctla4/cd28リガンド及びその使用 - Google Patents
新規のctla4/cd28リガンド及びその使用Info
- Publication number
- JP2000500961A JP2000500961A JP9501464A JP50146497A JP2000500961A JP 2000500961 A JP2000500961 A JP 2000500961A JP 9501464 A JP9501464 A JP 9501464A JP 50146497 A JP50146497 A JP 50146497A JP 2000500961 A JP2000500961 A JP 2000500961A
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- cells
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- human
- cell
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.B7−2融合タンパク質を符号化する分離核酸であって、B7−2活性を 持つ第1ペプチドを符号化するヌクレオチド配列と、第1ペプチドの可溶性、結 合親和性、又は結合価を変更する部分に対応する第2ペプチドを符号化するヌク レオチド配列と、からなる分離核酸。 2.DNAである請求項1に記載の分離核酸。 3.第1ペプチドが、ヒトB7−2タンパク質の細胞外ドメインを含む、請求項 2に記載の分離核酸。 4.第1ペプチドが、図8(配列同定番号第2番)に示した配列のアミノ酸残基 24乃至245からなる、請求項3に記載の分離核酸。 5.第1ペプチドが、ヒトB7−2の可変部様ドメインからなる、請求項3に記 載の分離核酸。 6.第1ペプチドが、ヒトB7−2の不変部様ドメインからなる、請求項3に記 載の分離核酸。 7.第2ペプチドが、免疫グロブリン不変部からなる、請求項2に記載の分離核 酸。 8.免疫グロブリン不変部が、ヒンジ、CH2、CH3領域を含むCγ1ドメイ ンである、請求項7に記載の分離核酸。 9.免疫グロブリン不変部が、不変部媒介生物学的エフェクタ機能を低下させる ように改質される、請求項7に記載の分離核酸。 10.生物学的エフェクタ機能が、補完活性化、Feレセプター相互作用、及び 補完活性化、及びFeレセプター相互作用からなる群から選択された、請求項9 に記載の分離核酸。 11.免疫グロブリン不変部が、ヒンジ、CH2、CH3領域を含むCγ4ドメ インである、請求項10に記載の分離核酸。 12.CH2の少なくとも一つのアミノ酸残基が、置換、付加、又は欠失によっ て改質される請求項11に記載の分離核酸。 13.B7−2活性を持つ第1ペプチドと、第1ペプチドの可溶性、結合親和性 、又は結合価を変更する部分に対応する第2ペプチドと、からなる分離B7−2 融合タンパク質。 14.第1ペプチドが、ヒトB7−2タンパク質の細胞外ドメインを含む、請求 項13に記載の分離B7−2融合タンパク質。 15.第1ペプチドが、図8(配列同定番号第2番)に示した配列のアミノ酸残 基24乃至245からなる、請求項14に記載の分離B7−2融合タンパク質。 16.第1ペプチドが、ヒトB7−2の可変部様ドメインからなる、請求項14 に記載の分離B7−2融合タンパク質。 17.第1ペプチドが、ヒトB7−2の不変部様ドメインからなる、請求項14 に記載の分離B7−2融合タンパク質。 18.第2ペプチドが、免疫グロブリン不変部からなる、請求項13に記載の分 離B7−2融合タンパク質。 19.免疫グロブリン不変部が、ヒンジ、CH2、CH3領域を含むCγ1ドメ インである、請求項18に記載の分離B7−2融合タンパク質。 20.免疫グロブリン不変部が、不変部媒介生物学的エフェクタ機能を低下させ るように改質される、請求項18に記載の分離B7−2融合タンパク質。 21.生物学的エフェクタ機能が、補完活性化、Feレセプター相互作用、及び 補完活性化、及びFeレセプター相互作用からなる群から選択された、請求項2 0に記載の分離B7−2融合タンパク質。 22.免疫グロブリン不変部が、ヒンジ、CH2、CH3領域を含むCγ4ドメ インである、請求項21に記載の分離B7−2融合タンパク質。 23.CH2ドメインの少なくとも一つのアミノ酸残基が、置換、付加、又は欠 失によって改質される請求項21に記載の分離B7−2融合タンパク質。 24.請求項13に記載の融合タンパク質及び薬学的に適格なキャリアからなる 薬剤投与に適した組成物。 25.請求項14に記載の融合タンパク質及び薬学的に受容可能なキャリアから なる薬剤投与に適した組成物。 26.請求項16に記載の融合タンパク質及び薬学的に適格なキャリアからなる 薬剤投与に適した組成物。 27.請求項18に記載の融合タンパク質及び薬学的に適格なキャリアからなる 薬剤投与に適した組成物。 28.Bリンパ球抗原であるB7−2と、免疫細胞の表面上のその抗原の本来の リガンドとの相互作用を阻止する方法であって、 免疫細胞を、B7−2がその本来のリガンドと結合するのを抑制するB7−2 融合タンパク質と接触させ、よってB7−2リガンド相互作用を通じてその免疫 細胞の相互促進を抑制することを含む、相互作用を抑制する方法。 29.B7−2融合タンパク質が、B7−2活性を持つ第1ペプチドと、第1ペ プチドの可溶性、結合親和性、又は結合価を変更する部分から成る第2ぺプチド と、からなる、請求項28に記載の方法。 30.第1ペプチドが、ヒトB7−2タンパク質の細胞外ドメインを含む、請求 項29に記載の方法。 31.第1ペプチドが、図8(配列同定番号第2番)に示した配列のアミノ酸残 基24乃至245からなる、請求項30に記載の方法。 32.第2ペプチドが、免疫グロブリン不変部からなる、請求項29に記載の方 法。 33.免疫グロブリン不変部が、ヒンジ、CH2、CH3領域を含むCγ1ドメ インである、請求項32に記載の方法。 34.Bリンパ球抗原であるB7−2と免疫細胞の表面上のその抗原の本来のリ ガンドとの相互作用によって媒介される患者の自己免疫疾患を治療する方法であ って、 患者に抑制性の形態のB7−2融合タンパク質を投与し、よってB7−2リガ ンド相互作用を通じてその免疫細胞の相互促進を抑制することを含む、自己免疫 疾患を治療する方法。 35.抑制性の形態のB7−2融合タンパク質が、B7−2タンパク質の細胞外 ドメインを含む第1ペプチドと、免疫グロブリン固定ドメインを含む第2ペプチ ドと、を含むB7−2免疫グロブリン融合タンパク質(B7−2Ig)である、 請求項34に記載の方法。 36.B7−2タンパク質の細胞外ドメインが、図8(配列同定番号第2番)に 示した配列のアミノ酸残基24乃至245からなる、請求項35に記載の方法。 37.Bリンパ球抗原であるB7−2と免疫細胞の表面上のその抗原の本来のリ ガンドとの相互作用によって媒介される患者のアレルギーを治療する方法であっ て、 患者に抑制性の形態のB7−2融合タンパク質を投与し、よってB7−2リガ ンド相互作用を通じてその免疫細胞の相互促進を抑制することを含む、アレルギ ーを治療する方法。 38.B7−2免疫グロブリン様可変部ドメインを含むが、B7−2免疫グロブ リン様不変部ドメインを含まない、B細胞活性化抗原B7−2の分離可変部型。 39.ヒトのものである、請求項38に記載のB7−2可変部型。 40.異形のポリペプチドに機能的に結合しているB7−2可変部ポリペプチド を含む融合タンパク質である、請求項38に記載のB7−2可変部型。 41.B7−2可変部ポリペプチドが、ヒトB7−2可変部ポリペプチドである 、請求項40に記載のB7−2可変部型。 42.ヒトB7−2可変部ポリペプチドが、配列同定番号第2番の概24乃至1 33位置のアミノ酸配列を含む、請求項41のB7−2可変部型。 43.異形のポリペプチドが、免疫グロブリン不変部を含む、請求項40に記載 のB7−2可変部型。 44.免疫グロブリン不変部が、IgG1のヒンジ、CH2、CH3領域を含む 、請求項43に記載のB7−2可変部型。 45.膜内外ドメインに機能的に結合しているB7−2免疫グロブリン様可変部 ドメインを含み、B7−2可変部型が細胞表面に発現している、請求項38に記 載のB7−2可変部型。 46.B7−2免疫グロブリン様可変部ドメインと膜内外ドメインとの間に位置 する非B7−2リンカポリペプチドを更に含む、請求項45に記載のB7−2可 変部型。 47.細胞形質のドメインを更に含む、請求項45のB7−2可変部型。 48.固体支持体に結合されたB7−2免疫グロブリン様可変部ドメインを含む 、請求項38に記載のB7−2可変部型。 49.固体支持体が、ビードまたはプレートである、請求項48に記載のB7− 2可変部型。 50.B7−2免疫グロブリン様可変部ドメインと固体支持体との間に位置する 非B7−2リンカポリペプチドを更に含む、請求項48に記載のB7−2可変部 型。 51.異形のポリペプチドに機能的に結合しているヒトB7−2免疫グロブリン 様可変部ドメインを含む分離B7−2融合タンパク質であって、この分離B7− 2融合タンパク質が、B7−2免疫グロブリン様不変部ドメインを含まない、B 7−2融合タンパク質。 52.ヒトB7−2免疫グロブリン様可変部ドメインが、配列同定番号第2番の 概24乃至133位置のアミノ酸配列を含む、請求項51のB7−2融合タンパ ク質。 53.異形のポリペプチドが、免疫グロブリン不変部ポリペプチドを含む、請求 項51に記載のB7−2融合タンパク質。 54.分離B7−2融合タンパク質の可変部型を符号化する分離核酸分子であっ て、このB7−2融合タンパク質が、異形のポリペプチドに機能的に結合してい るヒトB7−2免疫グロブリン様可変部ドメインを含み、B7−2免疫グロブリ ン様不変部ドメインを含まない、分離核酸分子。 55.異形のポリペプチドが、免疫グロブリン不変部ポリペプチドである、請求 項58に記載の核酸。 56.請求項54に記載の核酸分子を含む組換え発現べクター。 57.請求項56に記載の組換え発現べクターを含む宿主細胞。 58.B7−2の可変部型を符号化する分離核酸分子であって、この核酸が、信 号ペプチドと、ヒトB7−2免疫グロブリン様可変部ドメインと、膜内外ドメイ ンと、細胞形質ドメインとを符号化する隣接のヌクレオチド配列を含む、分離核 酸分子。 59.ヒトB7−2免疫グロブリン様可変部ドメインが、配列同定番号第2番の 概24乃至133位置のアミノ酸配列を含む、請求項58に記載の核酸分子。 60.B7−2免疫グロブリン様可変部ドメインを符号化するヌクレオチド配列 と膜内外ドメインとの間に位置する非B7−2リンカポリペプチドを符号化する ヌクレオチド配列を更に含む、請求項58の核酸分子。 61.請求項58に記載の核酸分子を含む組換え発現ベクター。 62.請求項61に記載の組換え発現ベクターを含む宿主細胞であって、B7− 2の可変部型が細胞表面に発現された、宿主細胞。 63.活性化T細胞によって反応を促進する方法であって、活性化T細胞をB細 胞活性化抗原B7−2の可変部型と接触させることを含み、このB7−2の可変 部型が、活性化T細胞による反応が促進されるように、B7−2免疫グロブリン 様可変部ドメインを含むが、B7−2免疫グロブリン様不変部ドメインを含まな い、反応を促進する方法。 64.ThelperType2(TH2)反応が、優先的に促進される、請求項63に 記載の方法。
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PCT/US1996/009052 WO1996040915A2 (en) | 1995-06-07 | 1996-06-06 | Novel ctla4/cd28 ligands and uses therefor |
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1996
- 1996-06-06 CA CA002222999A patent/CA2222999A1/en not_active Abandoned
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- 1996-06-06 WO PCT/US1996/009052 patent/WO1996040915A2/en active IP Right Grant
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2007
- 2007-12-19 JP JP2007327832A patent/JP2008142084A/ja active Pending
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DE69636890D1 (de) | 2007-03-22 |
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ATE353362T1 (de) | 2007-02-15 |
AU6255496A (en) | 1996-12-30 |
EP1806402A3 (en) | 2007-09-19 |
US20040230051A1 (en) | 2004-11-18 |
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WO1996040915A2 (en) | 1996-12-19 |
JP2008142084A (ja) | 2008-06-26 |
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