JP2000500023A - 正しく折畳まれた生物学的に活性の組換えタンパク質の製造方法 - Google Patents
正しく折畳まれた生物学的に活性の組換えタンパク質の製造方法Info
- Publication number
- JP2000500023A JP2000500023A JP9518783A JP51878397A JP2000500023A JP 2000500023 A JP2000500023 A JP 2000500023A JP 9518783 A JP9518783 A JP 9518783A JP 51878397 A JP51878397 A JP 51878397A JP 2000500023 A JP2000500023 A JP 2000500023A
- Authority
- JP
- Japan
- Prior art keywords
- igf
- protein
- cells
- buffer
- correctly folded
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormones [GH] (Somatotropin)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/65—Insulin-like growth factors (Somatomedins), e.g. IGF-1, IGF-2
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Endocrinology (AREA)
- Genetics & Genomics (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Diabetes (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.(a)原核細胞中でタンパク質またはポリペプチドを発現させる段階と、 (b)細胞を採取する段階と、 (c)pH約8〜11のバッファ中でカオトロピック剤及び還元剤によって細胞 を直接可溶化する段階と、 (d)水及び希釈剤で希釈する段階と、 から成る正しく折畳まれた生物学的に活性の組換えタンパク質またはポリペプチ ドの製造方法。 2.タンパク質が、IGF−I、IGF−IIまたはGHであることを特徴とす る請求項1に記載の方法。 3.タンパク質がIGF−Iであり、 (a)原核細胞系、好ましくは大腸菌中でIGF−I−融合タンパク質を発現さ せる段階と、 (b)細胞を採取する段階と、 (c)pH8〜11のバッファ中でカオトロピック剤及び還元剤によって細胞を 直接可溶化する段階と、 (d)水及び希釈剤で希釈する段階と、 (e)開裂剤を添加する段階と、 (f)生物活性IGF−Iを産生するために精製する段階と、から成ることを特 徴とする請求項1に記載の方法。 4.IGF−I−融合タンパク質がハイブリッドZ−IGF−Iであることを特 徴とする請求項1から3のいずれか一項に記載の方法。 5.段階(c)のバッファがトリスまたはグリシンであることを特徴とする請求 項1から4のいずれか一項に記載の方法。 6.段階(c)のpHが約8であることを特徴とする請求項1から5のいずれか 一項に記載の方法。 7.段階(c)のカオトロピック剤がグアニジンまたは尿素であることを特徴と する請求項1から6のいずれか一項に記載の方法。 8.グアニジンを濃度3〜7Mで使用することを特徴とする請求項7に記載の方 法。 9.段階(c)の還元剤がDTTまたはシステインであることを特徴とする請求 項1から8のいずれか一項に記載の方法。 10.段階(d)の希釈剤がエタノールであることを特徴とする請求項1から9 のいずれか一項に記載の方法。 11.段階(d)後のpHが、好ましくはpH6未満、より好ましくはpH3以 下であることを特徴とする請求項1から10のいずれか一項に記載の方法。 12.段階(d)と(e)との間でpHを3以下に下げることを特徴とする請求 項11に記載の方法。 13.段階(d)と(e)との間に濃縮段階とバッファ交換とを行うことを特徴 とする請求項3に記載の方法。 14.クロマトグラフィー及び/またはイオン交換を使用することを特徴とする 請求項13に記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE9504019-2 | 1995-11-13 | ||
SE9504019A SE9504019D0 (sv) | 1995-11-13 | 1995-11-13 | Method for production of proteins |
PCT/SE1996/001456 WO1997018233A1 (en) | 1995-11-13 | 1996-11-12 | Method for producing a correctly folded, biological active recombinant protein |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2000500023A true JP2000500023A (ja) | 2000-01-11 |
JP3930051B2 JP3930051B2 (ja) | 2007-06-13 |
Family
ID=20400188
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP51878397A Expired - Fee Related JP3930051B2 (ja) | 1995-11-13 | 1996-11-12 | 正しく折畳まれた生物学的に活性の組換えタンパク質の製造方法 |
Country Status (11)
Country | Link |
---|---|
US (1) | US6605706B1 (ja) |
EP (1) | EP0952981B1 (ja) |
JP (1) | JP3930051B2 (ja) |
AT (1) | ATE261985T1 (ja) |
AU (1) | AU705192B2 (ja) |
CA (1) | CA2236751C (ja) |
DE (1) | DE69631906T2 (ja) |
HK (1) | HK1023781A1 (ja) |
NO (1) | NO320175B1 (ja) |
SE (1) | SE9504019D0 (ja) |
WO (1) | WO1997018233A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2022097216A1 (ja) * | 2020-11-05 | 2022-05-12 | シミックホールディングス株式会社 | 組み換えインスリン様成長因子iの製造方法 |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6653098B1 (en) | 1998-02-23 | 2003-11-25 | G. D. Searle & Co. | Method of producing mouse and human endostatin |
WO1999050302A1 (en) * | 1998-03-31 | 1999-10-07 | Tonghua Gantech Biotechnology Ltd. | Chimeric protein containing an intramolecular chaperone-like sequence and its application to insulin production |
GB9913437D0 (en) * | 1999-06-09 | 1999-08-11 | Medical Res Council | Fusion proteins |
FR2820424B1 (fr) * | 2001-02-05 | 2004-01-02 | Merieux Oravax | Procede de purification de alpa |
CN101506222B (zh) | 2006-07-14 | 2013-10-02 | 健泰科生物技术公司 | 重组蛋白的重折叠 |
US9119832B2 (en) | 2014-02-05 | 2015-09-01 | The Regents Of The University Of California | Methods of treating mild brain injury |
WO2017075535A1 (en) | 2015-10-28 | 2017-05-04 | Oxeia Biopharmaceuticals, Inc. | Methods of treating neurodegenerative conditions |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SE8505922D0 (sv) * | 1985-12-13 | 1985-12-13 | Kabigen Ab | Construction of an igg binding protein to facilitate downstream processing using protein engineering |
US5231178A (en) | 1991-01-16 | 1993-07-27 | The Salk Institute Biotechnology/Industrial Associates, Inc. | Method for the purification of intact, correctly-folded insulin-like growth factor-1 |
US5288931A (en) * | 1991-12-06 | 1994-02-22 | Genentech, Inc. | Method for refolding insoluble, misfolded insulin-like growth factor-I into an active conformation |
DK0600372T3 (da) * | 1992-12-02 | 1997-08-11 | Hoechst Ag | Fremgangsmåde til fremstilling af proinsulin med korrekt forbundne cystinbroer. |
US5407810A (en) * | 1993-08-20 | 1995-04-18 | Genentech, Inc. | Aqueous multiple-phase isolation of polypeptide |
US5663304A (en) * | 1993-08-20 | 1997-09-02 | Genentech, Inc. | Refolding of misfolded insulin-like growth factor-I |
-
1995
- 1995-11-13 SE SE9504019A patent/SE9504019D0/xx unknown
-
1996
- 1996-11-12 JP JP51878397A patent/JP3930051B2/ja not_active Expired - Fee Related
- 1996-11-12 AT AT96939406T patent/ATE261985T1/de not_active IP Right Cessation
- 1996-11-12 WO PCT/SE1996/001456 patent/WO1997018233A1/en active IP Right Grant
- 1996-11-12 US US09/051,941 patent/US6605706B1/en not_active Expired - Fee Related
- 1996-11-12 AU AU76596/96A patent/AU705192B2/en not_active Ceased
- 1996-11-12 DE DE69631906T patent/DE69631906T2/de not_active Expired - Fee Related
- 1996-11-12 CA CA002236751A patent/CA2236751C/en not_active Expired - Fee Related
- 1996-11-12 EP EP96939406A patent/EP0952981B1/en not_active Expired - Lifetime
-
1998
- 1998-05-12 NO NO19982155A patent/NO320175B1/no not_active IP Right Cessation
-
2000
- 2000-04-26 HK HK00102454A patent/HK1023781A1/xx not_active IP Right Cessation
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2022097216A1 (ja) * | 2020-11-05 | 2022-05-12 | シミックホールディングス株式会社 | 組み換えインスリン様成長因子iの製造方法 |
Also Published As
Publication number | Publication date |
---|---|
NO320175B1 (no) | 2005-11-07 |
WO1997018233A1 (en) | 1997-05-22 |
CA2236751C (en) | 2007-01-09 |
NO982155D0 (no) | 1998-05-12 |
JP3930051B2 (ja) | 2007-06-13 |
NO982155L (no) | 1998-05-12 |
US6605706B1 (en) | 2003-08-12 |
ATE261985T1 (de) | 2004-04-15 |
AU7659696A (en) | 1997-06-05 |
EP0952981B1 (en) | 2004-03-17 |
DE69631906T2 (de) | 2005-01-20 |
EP0952981A1 (en) | 1999-11-03 |
SE9504019D0 (sv) | 1995-11-13 |
CA2236751A1 (en) | 1997-05-22 |
AU705192B2 (en) | 1999-05-20 |
HK1023781A1 (en) | 2000-09-22 |
DE69631906D1 (de) | 2004-04-22 |
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