CA2236751A1 - Method for producing a correctly folded, biological active recombinant protein - Google Patents

Method for producing a correctly folded, biological active recombinant protein

Info

Publication number
CA2236751A1
CA2236751A1 CA002236751A CA2236751A CA2236751A1 CA 2236751 A1 CA2236751 A1 CA 2236751A1 CA 002236751 A CA002236751 A CA 002236751A CA 2236751 A CA2236751 A CA 2236751A CA 2236751 A1 CA2236751 A1 CA 2236751A1
Authority
CA
Canada
Prior art keywords
producing
recombinant protein
biological active
correctly folded
active recombinant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002236751A
Other languages
French (fr)
Other versions
CA2236751C (en
Inventor
Jan-Gunnar Gustafsson
Johan Ohman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Health AB
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2236751A1 publication Critical patent/CA2236751A1/en
Application granted granted Critical
Publication of CA2236751C publication Critical patent/CA2236751C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Endocrinology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Diabetes (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a method for producing a correctly folded, biological active recombinant protein or polypeptide, comprising the steps of expression of the protein in prokaryotic cells, harvest of the cells, direct solubilization of the cells in a buffer at pH
about 8 to 11 and thereafter dilution with water and a diluent. The protein or polypeptide is preferably GH, IGF-I or IGF-II.
CA002236751A 1995-11-13 1996-11-12 Method for producing a correctly folded, biological active recombinant protein Expired - Fee Related CA2236751C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE9504019-2 1995-11-13
SE9504019A SE9504019D0 (en) 1995-11-13 1995-11-13 Method for production of proteins
PCT/SE1996/001456 WO1997018233A1 (en) 1995-11-13 1996-11-12 Method for producing a correctly folded, biological active recombinant protein

Publications (2)

Publication Number Publication Date
CA2236751A1 true CA2236751A1 (en) 1997-05-22
CA2236751C CA2236751C (en) 2007-01-09

Family

ID=20400188

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002236751A Expired - Fee Related CA2236751C (en) 1995-11-13 1996-11-12 Method for producing a correctly folded, biological active recombinant protein

Country Status (11)

Country Link
US (1) US6605706B1 (en)
EP (1) EP0952981B1 (en)
JP (1) JP3930051B2 (en)
AT (1) ATE261985T1 (en)
AU (1) AU705192B2 (en)
CA (1) CA2236751C (en)
DE (1) DE69631906T2 (en)
HK (1) HK1023781A1 (en)
NO (1) NO320175B1 (en)
SE (1) SE9504019D0 (en)
WO (1) WO1997018233A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6653098B1 (en) * 1998-02-23 2003-11-25 G. D. Searle & Co. Method of producing mouse and human endostatin
US6924120B1 (en) 1998-03-31 2005-08-02 Tonghua Gantech Biotechnology, Ltd. Chaperone protein containing an intramolecular chaperone-like sequence and its application to insulin production
GB9913437D0 (en) * 1999-06-09 1999-08-11 Medical Res Council Fusion proteins
FR2820424B1 (en) * 2001-02-05 2004-01-02 Merieux Oravax ALPA PURIFICATION PROCESS
AU2007272412B2 (en) 2006-07-14 2013-11-07 Genentech, Inc. Refolding of recombinant proteins
US9119832B2 (en) 2014-02-05 2015-09-01 The Regents Of The University Of California Methods of treating mild brain injury
WO2017075535A1 (en) 2015-10-28 2017-05-04 Oxeia Biopharmaceuticals, Inc. Methods of treating neurodegenerative conditions
KR20230098304A (en) * 2020-11-05 2023-07-03 시믹 홀딩스 씨오., 엘티디. Method for producing recombinant insulin-like growth factor I

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE8505922D0 (en) * 1985-12-13 1985-12-13 Kabigen Ab CONSTRUCTION OF AN IGG BINDING PROTEIN TO FACILITATE DOWNSTREAM PROCESSING USING PROTEIN ENGINEERING
US5231178A (en) 1991-01-16 1993-07-27 The Salk Institute Biotechnology/Industrial Associates, Inc. Method for the purification of intact, correctly-folded insulin-like growth factor-1
US5288931A (en) * 1991-12-06 1994-02-22 Genentech, Inc. Method for refolding insoluble, misfolded insulin-like growth factor-I into an active conformation
DK0600372T3 (en) * 1992-12-02 1997-08-11 Hoechst Ag Process for the preparation of proinsulin with properly connected cystine bridges.
US5663304A (en) * 1993-08-20 1997-09-02 Genentech, Inc. Refolding of misfolded insulin-like growth factor-I
US5407810A (en) 1993-08-20 1995-04-18 Genentech, Inc. Aqueous multiple-phase isolation of polypeptide

Also Published As

Publication number Publication date
WO1997018233A1 (en) 1997-05-22
ATE261985T1 (en) 2004-04-15
NO320175B1 (en) 2005-11-07
HK1023781A1 (en) 2000-09-22
JP2000500023A (en) 2000-01-11
SE9504019D0 (en) 1995-11-13
US6605706B1 (en) 2003-08-12
EP0952981B1 (en) 2004-03-17
NO982155L (en) 1998-05-12
AU705192B2 (en) 1999-05-20
AU7659696A (en) 1997-06-05
JP3930051B2 (en) 2007-06-13
DE69631906T2 (en) 2005-01-20
CA2236751C (en) 2007-01-09
NO982155D0 (en) 1998-05-12
DE69631906D1 (en) 2004-04-22
EP0952981A1 (en) 1999-11-03

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