JP2000245832A - Method for inactivating virus - Google Patents
Method for inactivating virusInfo
- Publication number
- JP2000245832A JP2000245832A JP11100474A JP10047499A JP2000245832A JP 2000245832 A JP2000245832 A JP 2000245832A JP 11100474 A JP11100474 A JP 11100474A JP 10047499 A JP10047499 A JP 10047499A JP 2000245832 A JP2000245832 A JP 2000245832A
- Authority
- JP
- Japan
- Prior art keywords
- human body
- plasma components
- blood
- cells
- inactivating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3681—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits by irradiation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3678—Separation of cells using wave pressure; Manipulation of individual corpuscles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3687—Chemical treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3687—Chemical treatment
- A61M1/3689—Chemical treatment by biological cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、ＨＩＶ、ＨＢＶ、ＨＣ
Ｖ、ＨＴＬＶ−１、ＨＴＬＶ−２などの、レトロウイル
スに感染している患者の命を、延命させる治療手段と、
又は肝臓機能障害（肝不全）、又は腎臓機能障害（腎不
全）の患者の命を、延命させるための治療手段に必要な
技術に関するものである。The present invention relates to HIV, HBV, HC
V, HTLV-1, HTLV-2, etc., a therapeutic means for extending the life of a patient infected with a retrovirus,
Alternatively, the present invention relates to a technique required for a therapeutic means for extending the life of a patient with liver dysfunction (liver failure) or kidney dysfunction (renal failure).
【０００２】本発明は、ＨＩＶ、ＨＢＶ、ＨＣＶ、ＨＴ
ＬＶ−１、ＨＴＬＶ−２などの、レトロウイルスに感染
している患者の命を延命させる治療手段と、又は肝臓機
能障害（肝不全）又は腎臓機能障害（腎不全）の患者の
命を延命させるための治療手段を提供することを目的と
する。[0002] The present invention relates to HIV, HBV, HCV, HT
Remedies to prolong the life of patients infected with a retrovirus, such as LV-1, HTLV-2, or to prolong the life of patients with liver dysfunction (liver failure) or kidney dysfunction (renal failure) The purpose is to provide a therapeutic means.
【０００３】[0003]
【課題を解決するための手段】人体の血液を、血液中の
細胞である、赤血球、白血球、血小板などの細胞部分
と、血液中の溶液である、血漿成分とに分離したあと、
血漿成分中に、超音波振動が発生する、波動エネルギー
を使用して、血漿成分中に存在するレトロウイルス、例
えば、ＨＩＶ、ＨＢＶ、ＨＣＶ、ＨＴＬＶ−１、ＨＴＬ
Ｖ−２又は異種動物が保菌している，種々のレトロウイ
ルス、例えば、ブタ内在性レトロウイルス（ＰＥＲＶ）
又は細菌を、超音波振動の波動エネルギーを使用して不
活化（殺す）することで、異種動物の、さまざまな臓器
を、再利用することが出来るようにして、人体の命を延
命させるための治療手段として、異種動物の臓器を利用
する。Means for Solving the Problems After the blood of the human body is separated into cells in blood, such as red blood cells, white blood cells, and platelets, and plasma components in the blood,
Ultrasonic vibrations are generated in the plasma component, and using the wave energy, retroviruses present in the plasma component, such as HIV, HBV, HCV, HTLV-1, HTL
V-2 or various retroviruses harbored by a heterologous animal, for example, porcine endogenous retrovirus (PERV)
Or, by inactivating (killing) bacteria using the wave energy of ultrasonic vibration, it is possible to reuse various organs of foreign animals and extend the life of the human body. Heterologous organs are used as therapeutic means.
【０００４】[0004]
【作用】超音波振動は、溶液中において、圧力波となり
キャビテーションを引き起こす、キャビテーションと
は、溶液中での局所的な圧力低下により形成される、無
数の極めて小さな気泡が連続して潰れることを言い、圧
力波のサイクルが負の時に形成された気泡が、正の時に
潰れ、溶液中の物質に、激しい衝撃を繰り返し与えるこ
とにより、細菌も、種々のウイルスも、さらには、血漿
成分中の重要な蛋白質、酵素、ホルモン、アミノ酸など
をも破砕するけれども、細菌及び種々のウイルスを破砕
（不活化）する、超音波振動の出力と、血漿成分中の蛋
白質、酵素、ホルモン、アミノ酸などを破砕する場合
の、超音波振動の出力を比較すると、細菌及び種々のウ
イルス、例えば、特にＨＩＶなどのレトロウイルスを破
砕する場合のほうが、血漿成分中の蛋白質、酵素、ホル
モン、アミノ酸などを破砕する場合よりも、超音波振動
の出力が、低くくてもよいことが判明した、特にレトロ
ウイルスは、外膜タンパク（ｅｎｖ遺伝子産物）を持っ
ている構造なので、超音波振動を使用しての破砕が効果
的であることが判明した、この現象を利用して、血漿成
分中の蛋白質、酵素、ホルモン、アミノ酸及びその他の
重要な血漿成分中の物質に影響を与えない範囲内の、超
音波振動の出力を使用して、血漿成分中に存在する、種
々の細菌と、種々のレトロウイルスである、ＨＩＶ、Ｈ
ＢＶ、ＨＣＶ、ＨＴＬＶ−１、ＨＴＬ−２又はその他の
異種動物のレトロウイルスを破砕（不活化）することが
出来る現象を、人体に関して、レトロウイルスが関係し
ている、人体の種々の難病の、延命効果のある、治療手
段として使用する。[Function] Ultrasonic vibration causes a pressure wave in a solution to cause cavitation. Cavitation is a phenomenon in which countless extremely small bubbles formed by a local pressure drop in a solution are continuously crushed. The bubbles formed when the pressure wave cycle is negative are crushed when the pressure wave cycle is negative, and repeatedly subject to violent impacts on the substances in the solution, so that bacteria, various viruses, and even plasma components are important. It crushes proteins, enzymes, hormones, amino acids, etc., but also crushes (inactivates) bacteria and various viruses, sonication output, and crushes proteins, enzymes, hormones, amino acids, etc. in plasma components. Comparing the output of ultrasonic vibrations in the case, it is better to disrupt bacteria and various viruses, for example, retroviruses such as HIV in particular. It has been found that the output of ultrasonic vibration may be lower than when crushing proteins, enzymes, hormones, amino acids, etc. in the plasma component. In particular, retroviruses use outer membrane proteins (env gene products) Because of its structure, crushing using ultrasonic vibration has been found to be effective. Utilizing this phenomenon, proteins, enzymes, hormones, amino acids and other important plasma components in plasma components Using the output of ultrasonic vibrations within a range that does not affect the substances in it, the various bacteria and various retroviruses, HIV, H, present in the plasma components
The phenomenon capable of disrupting (inactivating) retroviruses of BV, HCV, HTLV-1, HTL-2 or other heterologous animals is described in the context of the human body by the various incurable diseases of the human body, which are related to the retroviruses. Use as a treatment that has a prolonged life.
【０００５】[0005]
【実施例】図１から図３に関連して、本発明の実施例を
説明する。図１から図３は本例のウイルスを不活化する
手段を示す構造概要説明図で、図１、図２及び図３は縦
断面図で、図中、１は人体で、２、４、５、７及び８は
接続用チューブで、３は分離装置で、６は超音波発生装
置で、９は冷却装置で、１０は異種動物の肝細胞で、１
１及び１２は冷却水出入口で、１３は冷却用ジャケット
で、１４はセラミックス製オリフィスプレートで、１５
はＯリングである。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described with reference to FIGS. 1 to 3 are schematic structural explanatory views showing the means for inactivating the virus of the present example. FIGS. 1, 2 and 3 are longitudinal sectional views, in which 1 is a human body, 2, 4, 5 , 7 and 8 are connecting tubes, 3 is a separation device, 6 is an ultrasonic generator, 9 is a cooling device, 10 is hepatocytes of a different animal, and 1 is
1 and 12 are cooling water inlets and outlets, 13 is a cooling jacket, 14 is a ceramic orifice plate, 15
Is an O-ring.
【０００６】図１に示しているのは、人体の血液を体外
に取り出して、体外に取り出した、人体の血液を、体外
循環させて、血液中の細胞である、赤血球、白血球及び
血小板などの血液中の細胞と、血液中の溶液である、血
漿成分とに分離する、分離装置３にて、血液細胞と血漿
成分とに分離したあと、血漿成分中に存在する、フリー
状態の、ＨＩＶ、ＨＢＶ、ＨＣＶ、ＨＴＬＶ−１、ＨＴ
ＬＶ−２などのレトロウイルスを超音波発生装置６内部
を通過させて、超音波振動による波動エネルギーを使用
して、血漿成分中のレトロウイルス、及びその他のウイ
ルスを不活化したあと、血液中の細胞と血漿成分とを合
流させて、人体に還流することで、血液中に存在する、
フリー状態のレトロウイルスを不活化している状態を示
している。FIG. 1 shows that the blood of the human body is taken out of the body, the blood of the human body taken out of the body is circulated extracorporeally, and the cells in the blood, such as red blood cells, white blood cells and platelets, are collected. Separating into blood cells and plasma components, which are solutions in blood, into blood cells, after being separated into blood cells and plasma components by the separation device 3, HIV, which is present in the plasma components and is in a free state, HBV, HCV, HTLV-1, HT
After a retrovirus such as LV-2 is passed through the inside of the ultrasonic generator 6 and the wave energy of the ultrasonic vibration is used to inactivate the retrovirus and other viruses in the plasma component, By combining cells and plasma components and returning them to the human body, they are present in the blood,
This shows a state in which the free retrovirus is inactivated.
【０００７】図２に示しているのは、人体の血液を体外
に取り出して、体外に取り出した、人体の血液を、体外
循環させて、血液中の細胞である、赤血球、白血球及び
血小板などの血液中の細胞と、血液中の溶液である、血
漿成分とに分離する、分離装置３にて、血液細胞と血漿
成分とに分離したあと、異種動物である、例えば、ブタ
から摘出して活かしている、ブタの肝臓である、ブタの
肝細胞１０内部を通過させて、人体が排泄する老廃物、
例えば、アンモニアなどの排泄物を、ブタの肝細胞１０
を使用して分解させるか、又は種々の蛋白質、酵素、ホ
ルモン、アミノ酸などを、ブタの肝細胞１０に合成させ
た後、人体の血漿成分を、人体に還流する直前に、ブタ
の肝細胞１０内部にて、人体の血漿成分中に感染及び溶
解した、ブタが保菌している、種々のブタ内在性レトロ
ウイルス（人間に感染するレトロウイルス）を、超音波
発生装置６内部を通過させて、超音波振動による波動エ
ネルギーを使用して、人体の血漿成分中に感染及び溶解
している、ブタが保菌している、ブタ内在性レトロウイ
ルスを不活化したあと、血液中の細胞と血漿成分とを合
流させて、人体に還流することで、ブタが保菌してい
る、ブタのレトロウイルスによる影響を受けることな
く、ブタの肝細胞１０を使用して、人体の血漿成分中の
老廃物を、安全に、分解、又は種々の蛋白質、酵素、ホ
ルモン、アミノ酸などを合成させることが出来る状態を
示している。FIG. 2 shows that the blood of the human body is taken out of the body, the blood of the human body taken out of the body is circulated extracorporeally, and the cells in the blood, such as red blood cells, white blood cells and platelets, are collected. Separation into blood cells and blood plasma components, which is a solution in blood, and blood cells and blood plasma components in a separating device 3, which is then removed from a xenogeneic animal, for example, a pig, and utilized. Waste, which is passed through pig hepatocytes 10 which are pig liver and excreted by the human body,
For example, excretions such as ammonia are transferred to pig hepatocytes 10
, Or after synthesizing various proteins, enzymes, hormones, amino acids, and the like into the porcine hepatocytes 10, immediately before the blood components of the human body are returned to the human body. Inside, various porcine endogenous retroviruses (retroviruses that infect humans) that have been infected and dissolved in plasma components of the human body and are carried by pigs are passed through the inside of the ultrasonic generator 6, After inactivating the pig's endogenous retrovirus, which has been infected and dissolved in the plasma components of the human body using the ultrasonic wave vibration, and which is retained by the pig, the blood cells and plasma components Are combined and returned to the human body, so that the porcine hepatocytes 10 are used, without being affected by the porcine retrovirus, to remove waste products in the plasma components of the human body using the porcine hepatocytes 10. Safe, minutes Or various proteins indicate enzymes, hormones, a state in which it is possible to synthesize an amino acid or the like.
【０００８】図３に示しているのは、図１と図２に示し
ている超音波発生装置６を使用して、実際に、血漿成分
中に、連続して、超音波振動を発生させると、血漿成分
中の温度が上昇するので、血漿成分を冷却する場合の、
具体的な方法の、実施例の詳細構造図を示している。FIG. 3 shows that the ultrasonic generator 6 shown in FIGS. 1 and 2 is used to actually generate ultrasonic vibrations in a plasma component continuously. Since the temperature in the plasma component increases, when cooling the plasma component,
FIG. 3 shows a detailed structural diagram of an embodiment of a specific method.
【０００９】[0009]
【発明の効果】本発明を使用して、ＨＩＶ、ＨＢＶ、Ｈ
ＣＶ、ＨＴＬＶ−１、ＨＴＬＶ−２などの、レトロウイ
ルスに感染している患者の体温を、４２℃以上から４５
℃以内の温度に、人体の体温を上昇させると、リンパ節
（白血球）と非共有結合している、ＨＩＶなどのレトロ
ウイルスが、リンパ節より離脱、及び白血球の表面上よ
り離脱してフリー状態となり、血液中に、レトロウイル
スが溶解することが判明している。（上記のことは、米
国のＤｒ．Ｓｔｅｐｈｅｎ Ｒ．ＡｓｈがＡＳＡＩＯ
Ｊｏｕｒｎａｌ Ａｐｒｉｌ１９９７年にて発表してい
る）この血液中に溶解した、ＨＩＶなどのレトロウイル
スを、不活化（殺す）する手段として、血液中の細胞で
ある、赤血球、白血球及び血小板などの細胞と、血液中
の溶液である、血漿成分とに分離したあと、血漿成分中
に感染及び溶解している、ＨＩＶなどのレトロウイルス
を超音波振動の波動エネルギーを使用して不活化したあ
と、血液細胞と血漿成分とを合流させて、人体に還流さ
せる治療方法を、ＨＩＶなどに感染している、エイズ患
者に適用することで、エイズ患者が体内に保菌してい
る、エイズウイルスの総量を、例えば、１／１００以下
に低下させることが出来ることで、エイズ患者の発症を
遅延させて、エイズ患者の生命を、延命させる延命効果
のある治療手段となる。尚、ＨＩＶ、ＨＴＬＶ−１、Ｈ
ＴＬＶ−２などの患者の場合には、ＨＢＶ、ＨＣＶなど
で起こる、肝機能障害（肝不全）の患者ではないので、
肝臓機能が正常に活動している、このような患者の場合
には、血漿成分中に存在するＨＩＶなどのレトロウイル
スを、超音波振動の波動エネルギーを使用して、不活化
（殺す）する過程にて、血漿成分を構成している、蛋白
質、酵素、ホルモン及びアミノ酸などの人体にとって重
要な物質を、多少破壊しても、例えば、１ヶ月間に、一
度位ずつの割合にて、一回の治療を行なうのに、数時間
位の短い時間の範囲内において、超音波振動エネルギー
の波動エネルギーを使用して、血漿成分中の蛋白質、酵
素、ホルモン及びアミノ酸などを破壊しても、治療手段
が終了すれば、肝臓などの臓器が、再び蛋白質、酵素、
ホルモン、アミノ酸などを合成して再生するので、肝不
全の患者に使用する場合の、超音波振動の出力よりも、
ＨＩＶなどのレトロウイルスに感染している患者の場合
には、より一段と強力な超音波振動の波動エネルギーを
使用して、ＨＩＶ、ＨＴＬＶ−１、ＨＴＬＶ−２などの
レトロウイルスを殺す治療を行なっても、別に問題は起
こらない。According to the present invention, HIV, HBV, H
The body temperature of a patient infected with a retrovirus such as CV, HTLV-1, HTLV-2, etc. is increased from 42 ° C. or higher to 45 ° C.
When the human body temperature rises to a temperature within ℃, retroviruses such as HIV, which are non-covalently bound to lymph nodes (white blood cells), detach from the lymph nodes and detach from the surface of the white blood cells to become free. It has been found that retrovirus is dissolved in blood. (The above is due to the fact that Dr. Stephen R. Ash of the United States
As a means of inactivating (killing) retroviruses such as HIV dissolved in the blood, cells such as red blood cells, white blood cells, and platelets, which are cells in the blood, are disclosed in Journal April 1997. After being separated into plasma components, which are solutions in the blood, retroviruses such as HIV, which are infected and dissolved in the plasma components, are inactivated by using the wave energy of ultrasonic vibration, By combining the plasma component with a therapeutic method of refluxing the human body, by applying to an AIDS patient infected with HIV or the like, the AIDS patient is colonized by the AIDS patient, the total amount of the AIDS virus, for example, Being able to reduce it to 1/100 or less, it delays the onset of AIDS patients and prolongs the life of AIDS patients, making it a therapeutic means with a life-extending effect . In addition, HIV, HTLV-1, H
In the case of patients such as TLV-2, they are not patients with liver dysfunction (liver failure) caused by HBV, HCV, etc.
In the case of such a patient whose liver function is operating normally, the process of inactivating (killing) a retrovirus such as HIV present in plasma components using the wave energy of ultrasonic vibration. In the above, even if substances that are important to the human body, such as proteins, enzymes, hormones and amino acids, which make up the plasma components, are somewhat destroyed, for example, once a month Even if the protein, enzymes, hormones, amino acids and the like in the plasma components are destroyed using the wave energy of the ultrasonic vibration energy within a short time of several hours to perform the treatment of Is over, organs such as the liver are turned back into proteins, enzymes,
Because it synthesizes and regenerates hormones and amino acids, it can be used for patients with liver failure, rather than the output of ultrasonic vibration.
In the case of a patient infected with a retrovirus such as HIV, a treatment for killing a retrovirus such as HIV, HTLV-1, or HTLV-2 is performed by using more powerful wave energy of ultrasonic vibration. But no problem arises.
【００１０】さらに、本発明を使用して、肝臓機能障害
（肝不全）の患者の血液を体外循環させて、体外循環さ
せている血液を、血液中の細胞である、赤血球、白血球
及び血小板などの細胞と、血液中の溶液である、血漿成
分とに分離したあと、分離した血漿成分を、異種動物で
ある、例えば、ブタの肝臓を、そのまま活かしている状
態にて使用するか、又はブタから肝臓だけを摘出して、
肝細胞だけを活かしている、肝細胞内部を、人体の血漿
成分を、体外循環させて、人体の血漿成分中の老廃物、
例えば、アンモニアなどの物質を、ブタの肝細胞を使用
して分解させるか、又は種々の蛋白質、酵素、ホルモ
ン、アミノ酸などを合成させたあとの、人体の血漿成分
を、人体に還流させる直前に、超音波振動の波動エネル
ギーを使用して、人体の血漿成分が、ブタの肝細胞内部
を通過する段階にて、血漿成分中に感染及び溶解した、
ブタが保菌している、ブタ特有のブタ内在性レトロウイ
ルスを不活化したあと、血液細胞と血漿成分とを合流さ
せて、人体に血液を還流させる構成ならば、ブタが保菌
している、ブタのブタ内在性レトロウイルスに、人体が
汚染されることなく、ブタの肝臓である、ブタの肝細胞
を使用して、人体が排泄する老廃物を、安全に、分解、
及び再び種々の重要な物質を、ブタの肝細胞に合成させ
ることが出来るので、肝炎、肝臓ガンなどの病気にて起
こる、肝臓機能障害の患者の命を延命させる、血液浄化
システムとしての、治療手段として使用することが出来
る。Further, the blood of a patient with liver dysfunction (liver failure) is extracorporeally circulated using the present invention, and the extracorporeally circulated blood is converted into cells in the blood, such as red blood cells, white blood cells, and platelets. After separating the cells into a solution in blood and a plasma component, the separated plasma component is used as a xenogeneic animal, for example, the liver of a pig as it is, or From the liver,
The hepatocytes that utilize only hepatocytes, the blood components of the human body, extracorporeally circulate, waste products in the plasma components of the human body,
For example, a substance such as ammonia is decomposed using porcine hepatocytes, or after synthesizing various proteins, enzymes, hormones, amino acids, etc., immediately before the blood component of the human body is returned to the human body. Using the wave energy of the ultrasonic vibration, the plasma component of the human body was infected and dissolved in the plasma component at the stage of passing through the inside of the hepatic cell of the pig,
If the pig carries the pig, the pig's endogenous retrovirus specific to the pig is inactivated, and then the blood cells are combined with the plasma components to allow blood to flow back to the human body. Using the pig endogenous retrovirus, the human liver is not contaminated, using the pig liver, pig hepatocytes, to safely decompose waste products excreted by the human body,
And again, various important substances can be synthesized by porcine hepatocytes, so that hepatitis, liver cancer, and other diseases that cause liver dysfunction, prolong the life of patients, and serve as a blood purification system. It can be used as a means.
【００１１】さらに、血漿成分中に存在する、ＨＩＶ、
ＨＢＶ、ＨＣＶ、ＨＴＬＶ−１、ＨＴＬＶ−２、ブタレ
トロウイルスなどのレトロウイルスを不活化（殺す）す
るのに、超音波発生装置を、直列又は並列に配列した複
数の、超音波振動を発生させることが出来る、複数の超
音波発生装置内部を通過させることで、例えば、１ｍｌ
当り、１０８個程度の個数の、ＨＩＶなどレトロウイル
スが血漿成分中に混入していても、複数の超音波発生装
置内部を通過させることで、血漿成分中に存在するＨＩ
Ｖなどのレトロウイルスの数値を０まで落として、血漿
成分中に存在するＨＩＶなどのレトロウイルスを全て不
活化（殺す）することが出来る。[0011] Furthermore, HIV, which is present in plasma components,
In order to inactivate (kill) retroviruses such as HBV, HCV, HTLV-1, HTLV-2 and porcine retrovirus, a plurality of ultrasonic vibrations are generated by arranging ultrasonic generators in series or in parallel. By passing through the inside of a plurality of ultrasonic generators, for example, 1 ml
Even if about 10 8 retroviruses such as HIV are mixed in the plasma component, by passing through the inside of a plurality of ultrasonic generators, the HI existing in the plasma component is reduced.
By reducing the value of the retrovirus such as V to 0, it is possible to inactivate (kill) all retroviruses such as HIV present in the plasma components.
【００１２】さらに、本発明を使用して、腎臓機能障害
（腎不全）の患者の治療手段にも、異種動物の腎臓を使
用して、肝機能障害の治療手段の場合と同様に、本発明
の治療手段を使用して、腎臓機能障害の患者の命を延命
させる、安全な、血液浄化システムとしての、治療手段
としても使用することが出来る。Further, the present invention is applied to the treatment of patients with renal dysfunction (renal insufficiency) using the kidneys of xenogeneic animals in the same manner as the treatment of hepatic dysfunction using kidneys of different animals. Can be used as a safe, blood purification system and as a therapeutic means to prolong the life of patients with renal impairment.
【図１】 本発明の原理説明図で、図１は縦断面図であ
る。FIG. 1 is a view for explaining the principle of the present invention, and FIG. 1 is a longitudinal sectional view.
【図２】 本発明の実施例のウイルスを不活化する手段
を示す構造概要の縦断面図である。FIG. 2 is a longitudinal sectional view of a structural outline showing a means for inactivating a virus according to an embodiment of the present invention.
【図３】 本発明の実施例のウイルスを不活化する手段
を示す構造概要の縦断面図である。FIG. 3 is a longitudinal sectional view of a structural outline showing a means for inactivating a virus according to an embodiment of the present invention.
１ 人体 ２、４，５，７及び８ 血液細胞及び血漿成分を体外循
環させるための、接続用チューブ（略して、接続用チュ
ーブとする） ３ 血液分離装置（略して、分離装置とする） ６ 超音波発生装置 ９ 冷却装置 １０ 異種動物の肝臓（略して、肝細胞とする） １１及び１２ 冷却水出入口 １３ 冷却用ジャケット １４ セラミックス製オリフィスプレート １５ Ｏリング1 Human body 2, 4, 5, 7, and 8 Connection tube (abbreviated as connection tube) for extracorporeal circulation of blood cells and plasma components 3 Blood separation device (abbreviated as separation device) 6 Ultrasonic generator 9 Cooling device 10 Heterologous animal liver (abbreviated as hepatocytes) 11 and 12 Cooling water inlet / outlet 13 Cooling jacket 14 Ceramic orifice plate 15 O-ring
Claims (5)
て、血液中の細胞である、赤血球、、白血球、血小板な
どの細胞と、血漿成分とに分離し、分離した、血漿成分
中に超音波が発生する、波動（振動）エネルギーを使用
して、血漿成分中に存在する、ＨＩＶ、ＨＢＶ、ＨＣ
Ｖ、ＨＴＬＶ−１、ＨＴＬＶ−２、又はその他のレトロ
ウイルスを不活化した後、血液細胞と血漿成分とを合流
させ、血液を人体に還流させる、ウイルスを不活化する
方法。1. The blood of the human body is extracorporeally circulated and separated outside the body into cells such as red blood cells, white blood cells, and platelets, which are cells in the blood, and plasma components. HIV, HBV, HC present in plasma components using wave (vibration) energy generated by ultrasonic waves
V, HTLV-1, HTLV-2, or other retroviruses, and then inactivating the virus, in which blood cells and plasma components are combined and blood is returned to the human body.
て、血液中の細胞である、赤血球、白血球、血小板など
の細胞と、血漿成分とに分離し、分離した血漿成分を、
異種動物の肝臓である、例えば、活きているブタの肝臓
である、肝細胞内部を循環させて、ブタの肝細胞を使用
して、人間の血漿成分中に存在する老廃物である、例え
ば、アンモニアなどの分解を、活きているブタの肝細胞
を使用して分解させるか、又は種々の蛋白質、酵素、ホ
ルモン、アミノ酸などを合成させた後、人体の血漿成分
を、人体に還流する直前に、ブタの肝細胞内部にて、人
体の血漿成分中に、感染及び溶解した、ブタが保菌して
いる、種々のブタ内在性レトロウイルス（ＰＥＲＶ）
を、超音波振動が発生する、波動（振動）エネルギーを
使用して不活化したあと、血液細胞と、血漿成分とを合
流させ、人体に還流させる、ウイルスを不活化する方
法。2. The blood of the human body is extracorporeally circulated, and extracorporeally separated into cells such as cells in blood, such as red blood cells, white blood cells, and platelets, and plasma components.
The liver of a xenogeneic animal, e.g., a living pig liver, is a waste product present in human plasma components, circulating inside hepatocytes, using pig hepatocytes, e.g., After decomposing ammonia and the like using live pig hepatocytes, or after synthesizing various proteins, enzymes, hormones, amino acids, etc., immediately before returning the human body plasma components to the human body Various porcine endogenous retroviruses (PERVs) that are infected and dissolved in the plasma components of the human body, inside the porcine hepatocytes,
A method of inactivating a virus by inactivating using a wave (vibration) energy, which generates ultrasonic vibration, and then merging blood cells and plasma components to return to the human body.
い範囲内の、超音波振動の波動エネルギーを使用して、
ＨＩＶ、ＨＢＶ、ＨＣＶ、ＨＴＬＶ−１、ＨＴＬＶ−
２、又はその他のレトロウイルスを不活化する請求項１
から２に記載の、ウイルスを不活化する方法。3. Use of wave energy of ultrasonic vibration within a range that does not denature various proteins in plasma components,
HIV, HBV, HCV, HTLV-1, HTLV-
2. Inactivating 2 or other retroviruses.
3. The method for inactivating a virus according to items 2 to 2.
ると、超音波振動が発生する、波動エネルギーの作用に
より、血漿成分中の温度が上昇する、この温度を人体の
体温である、３７℃前後に冷却して維持し、人体に還流
する請求項１から３に記載の、ウイルスを不活化する方
法。4. When an ultrasonic vibration is generated in a plasma component, the ultrasonic vibration is generated. The temperature of the plasma component increases by the action of wave energy. This temperature is the body temperature of a human body. The method for inactivating a virus according to claim 1, wherein the virus is cooled to and maintained at about 37 ° C. and refluxed to a human body.
スを不活化するのに、直列、又は並列に配列した複数
の、超音波振動を発生させることが出来る、超音波発生
装置内部を通過させて、レトロウイルスを不活化して、
人体に還流する請求項１から４に記載の、ウイルスを不
活化する方法。5. In order to inactivate a retrovirus such as HIV in a plasma component, a plurality of ultrasonic vibrations arranged in series or in parallel can be generated by passing through an ultrasonic generator. , Inactivating the retrovirus,
5. The method for inactivating a virus according to claim 1, wherein the virus is returned to a human body.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11100474A JP2000245832A (en) | 1999-03-02 | 1999-03-02 | Method for inactivating virus |
| PCT/JP2000/001205 WO2000051665A1 (en) | 1999-03-02 | 2000-03-01 | Method for inactivating viruses |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11100474A JP2000245832A (en) | 1999-03-02 | 1999-03-02 | Method for inactivating virus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2000245832A true JP2000245832A (en) | 2000-09-12 |
Family
ID=14274922
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11100474A Pending JP2000245832A (en) | 1999-03-02 | 1999-03-02 | Method for inactivating virus |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JP2000245832A (en) |
| WO (1) | WO2000051665A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004103443A1 (en) * | 2003-05-22 | 2004-12-02 | Beijing Jingjing Medical Equipment Co., Ltd. | Method for inactivating virus in circular blood and its applictions in treating viral diseases |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH077648U (en) * | 1992-11-11 | 1995-02-03 | 正巳 内山 | Extracorporeal blood flow type AIDS sterilizer |
| JPH0788182A (en) * | 1993-09-14 | 1995-04-04 | Akihiro Fujimura | Body outside circulating blood medical treatment device |
| JPH105328A (en) * | 1996-04-26 | 1998-01-13 | Nippon Ham Kk | Inactivation treatment of virus and device therefor |
-
1999
- 1999-03-02 JP JP11100474A patent/JP2000245832A/en active Pending
-
2000
- 2000-03-01 WO PCT/JP2000/001205 patent/WO2000051665A1/en unknown
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004103443A1 (en) * | 2003-05-22 | 2004-12-02 | Beijing Jingjing Medical Equipment Co., Ltd. | Method for inactivating virus in circular blood and its applictions in treating viral diseases |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2000051665A1 (en) | 2000-09-08 |
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