JP2001340452A - Production method of apparatus and blood preparation which inactivate virus - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an apparatus for life prolonging of patients infected with a retrovirus such as HIV, HBV, HCV, HTLV-1, HTLV-2 or PERV or patients with liver dysfunction (hepatic failure) or renal dysfunction (renal insufficiency). SOLUTION: The apparatus device which inactivates a virus consists of connection tubes 2, 7 and a pump 19 that take out blood from a human body and flow it back to the body and an ultrasonicator 6 that generates ultrasonic wave or ultrasonic irradiation to the whole blood flowing through a connection tube 2 with intensity in the range that does not destroy a cell in the blood such as erythrocyte, leucocyte or blood platelets. After inactivating HIV, HBV, HCV, HTLV-1, HTL-2 or other retroviruses existing in the whole blood by ultrasonic wave or ultrasonic irradiation by the ultrasonicator 6, the whole blood is flown back to the human body.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to HIV, HBV, HC
V, HTLV-1, HTLV-2, or PERV, for prolonging the life of a patient infected with a retrovirus or a patient with liver dysfunction (liver failure) or kidney dysfunction (renal failure) Regarding devices applied to treatment,
Furthermore, it relates to a method for producing a safe blood product.

[0002]

2. Description of the Related Art Conventionally, a patient infected with a retrovirus such as HIV has been administered a drug to suppress the onset of the disease, but inactivating (killing) those viruses in the blood. And was not an effective treatment. Although various studies have been conducted on drugs, there are also problems such as side effects, and it is considered that considerable time is required for the drugs to be useful.

[0003]

The present invention relates to HIV, H
BV, HCV, HTLV-1, HTLV-2, or PE
A therapeutic means for prolonging the life of a patient infected with a retrovirus, such as RV, or a patient with impaired liver function (liver failure) or impaired kidney function (renal failure);
An object is to provide a method for producing a safe blood product.

[0004]

According to the present invention, a retrovirus such as HIV, which is present in a free state in blood or in a non-covalent state on the surface of leukocytes, is inactivated (destructed) by ultrasonic vibration. Based on the finding that blood is drawn out of the human body outside the body and subjected to ultrasonic vibration and then circulated to the human body, the human body carries bacteria, and the human body carries HIV and other retroviruses. By reducing the numerical value and increasing the number of white blood cells, the onset is prevented beforehand.

[0005] Furthermore, the use of ultrasonic vibration or ultrasonic irradiation within a range that does not affect proteins, enzymes, hormones, amino acids, and the like in plasma components allows HIV,
Viruses other than retroviruses, such as various retroviruses such as HCV and parvovirus, are inactivated to produce safe blood products.

Specifically, the present invention relates to a connecting tube and a pump for extracting blood from a human body and circulating the blood into the human body, and adding red blood cells, which are cells in the blood, to whole blood flowing through the connecting tube. An ultrasonic generator for performing ultrasonic vibration or ultrasonic irradiation with an intensity within a range not destroying cells such as white blood cells and platelets, and comprising an ultrasonic vibration or ultrasonic irradiation by the ultrasonic generator, the whole blood , HBV, HCV, HTLV-1, HTL present in the blood of
After inactivating V-2 or other retroviruses,
It is a device that inactivates viruses, which allows whole blood to flow back to the human body.

Further, a plasma separator for separating cells, such as red blood cells, white blood cells, platelets, etc., which are cells in whole blood, taken from the human body before performing ultrasonic vibration or ultrasonic irradiation, and a plasma component. And means for joining the plasma component after the ultrasonic irradiation and the cells in the blood are provided.

Further, blood taken from the human body is passed through hepatocytes of a living liver of a different animal to decompose wastes present in human plasma components, and then subjected to ultrasonic vibration or ultrasonic vibration. Irradiation is performed. In this case, various proteins, enzymes, hormones, amino acids, and the like can be synthesized by the living hepatocytes of the liver.

Further, a cooling device is provided for suppressing a rise in blood temperature due to ultrasonic vibration or ultrasonic irradiation by the ultrasonic generator. In this case, the blood is cooled so as to keep the temperature of the blood at around 37 ° C., which is the body temperature of the human body.

[0010] Further, in order to inactivate retroviruses such as HIV in plasma components, a plurality of ultrasonic generators arranged in series or in parallel can be used.

According to the method for producing a blood product of the present invention, a plasma component is extracted from blood extracted from a human body, and an ultrasonic wave having an intensity within a range that does not affect proteins, enzymes, hormones, amino acids, etc. in the plasma component. Vibration or ultrasonic irradiation to give HIV, HBV, HCV, HTLV-
1. A safe blood product is produced by inactivating viruses other than retroviruses, such as HTLV-2 or other retroviruses, or parvovirus which is a pathogen virus of apple disease, and thereby producing safe blood products. .

[0012]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Cavitation or cavitation by ultrasonic vibration or ultrasonic irradiation according to Bernoulli's law is a pressure wave in a solution, which is formed by a local pressure drop in the solution. Countless extremely small bubbles are said to be continuously crushed, and the cycle of pressure waves is formed by the local pressure drop in the solution at negative times. It means crushing continuously at a positive time, and repeatedly subjecting the substance in the solution to violent impact. This cavitation disrupts bacteria and various viruses in solution, and also crushes important proteins, enzymes, hormones, amino acids, etc. in plasma components, but disrupts bacteria and various viruses. When the output of ultrasonic vibration is compared with the output of ultrasonic vibration when crushing proteins, enzymes, hormones, amino acids, etc. in plasma components, the output of ultrasonic vibration is higher in the former case than in the latter case. It turned out to be good even if it was low. Especially retroviruses,
Since it has a structure having an outer membrane protein (env gene product) (also called an envelope), it has been found that disruption using ultrasonic vibration is effective.

This phenomenon, that is, the use of ultrasonic vibration output within a range that does not affect the proteins, enzymes, hormones, amino acids and other important substances in the plasma component in the plasma component, The various bacteria present in the components and the various retroviruses HIV, HBV, HC
Retroviruses have been implicated in phenomena that can disrupt retroviruses, such as V, HTLV-1, HTLV-2, or other heterologous retroviruses, such as porcine endogenous retrovirus (PERV). It is used as a long-lasting and effective means of treating various incurable diseases of the human body. It can also be used as a technology for producing safe blood products.

Further, even if ultrasonic vibration or ultrasonic irradiation is used within a range that does not destroy cells, such as red blood cells, white blood cells, and platelets, which are cells in blood, the outer membrane proteins of retroviruses such as HIV ( env gene product), which destroys and inactivates retroviruses such as HIV. Whole blood taken out of the body can be used to remove red blood cells, white blood cells, platelets, and other cells from the blood. The retrovirus can be inactivated without separation into the plasma component which is a solution of the above.

Using the present invention, HIV, HBV, HC
V, HTLV-1, HTLV-2, etc., the temperature of a patient infected with a retrovirus is increased from 42 ° C. or higher to 45 ° C.
When the temperature is raised to within, a retrovirus such as HIV, which is non-covalently bound to lymph nodes or leukocytes, detaches from the lymph nodes, and detaches from the surface of leukocytes and becomes free, and In some cases it has been found that retroviruses lyse. (The above is based on the fact that Dr. Stephen R. Ash of the United States
(published in urnal April 1997)
As means for inactivating (killing) retroviruses such as HIV dissolved in the blood, cells in the blood, such as cells such as red blood cells, white blood cells, and platelets, and plasma components, which are solutions in the blood, After inactivation of retroviruses such as HIV, which have been infected and dissolved in plasma components, using the wave energy of ultrasonic vibrations, the blood cells and plasma components are merged, By applying a therapeutic method to AIDS patients infected with HIV or the like, the total amount of the AIDS virus that the AIDS patient carries in the body can be reduced to, for example, 1/100 or less. By being able to do so, the onset of AIDS patients can be delayed, and the life of the AIDS patients can be prolonged.

Note that HIV, HTLV-1, HTLV-
In the case of the patient No. 2 or the like, the patient is not a patient with hepatic dysfunction (liver failure) caused by HBV, HCV, etc., and thus the liver function is normally activated. For such patients,
Retroviruses such as HIV present in plasma components
In the process of inactivating (killing) using the wave energy of ultrasonic vibration, proteins, which constitute plasma components,
Some destruction of substances important to the human body, such as enzymes, hormones and amino acids, does not cause any problems. That is, for example, to perform one treatment at a rate of once every one month, within one month, within a short time range of several hours, using the wave energy of ultrasonic vibration energy, Even if the proteins, enzymes, hormones and amino acids in the components are destroyed, the organs such as the liver are functioning normally and the protein, enzymes, hormones, amino acids, etc. are synthesized and regenerated once the treatment is completed. I do. Therefore, in the case of a patient infected with a retrovirus such as HIV, more powerful wave energy of the ultrasonic vibration is used than in the output of the ultrasonic vibration when used in a patient with liver failure. , HIV, HTLV-1, HTLV-
Treatments that kill 2 or other retroviruses do not cause any problems.

Furthermore, using the present invention, blood of a patient with liver dysfunction (liver failure) is circulated extracorporeally, and the blood circulated extracorporeally is converted into cells in the blood, such as erythrocytes, leukocytes and platelets. After being separated into cells and a plasma component, which is a solution in the blood, the separated plasma component is passed through live hepatocytes to decompose waste products in the plasma components of the human body, such as ammonia, Reflux. As an animal utilizing this hepatocyte, a xenogeneic animal, for example, pig liver, is used in a state in which the pig is kept as it is, or only the liver is extracted from the pig, and the pig liver cell is used alone. can do.

In addition, the wave energy of the ultrasonic vibration is used immediately before the blood components of the human body are returned to the human body after various proteins, enzymes, hormones, amino acids and the like are synthesized in pig hepatocytes. When the plasma component of the human body passes through the inside of the hepatic cells of the pig, it inactivates and dissolves the plasma component and inactivates the pig endogenous porcine endogenous retrovirus (PERV) which is carried by the pig. After that, if blood cells and plasma components are combined and the blood is returned to the human body, the human body is not contaminated by the pig endogenous retrovirus (PERV) of the pig, which is carried by the pig. By using pig hepatocytes, which are pig livers, it is possible to safely decompose waste products excreted by the human body, and to synthesize various important substances again with pig hepatocytes. Hepatitis, liver Occur in diseases such as cancer,
It can be used as a therapeutic means as a blood purification system to prolong the life of patients with liver dysfunction.

Furthermore, HIV, which is present in plasma components,
A plurality of ultrasonic generators arranged in series or in parallel to inactivate (kill) retroviruses such as HBV, HCV, HTLV-1, HTLV-2, porcine endogenous retrovirus, etc. by passing the device inside, for example, per milliliter, of 10 about ^eight number, that retroviruses such as HIV are also be mixed in the plasma component, which is caused to pass through the inside multiple ultrasonic generator Then, the value of the retrovirus such as HIV existing in the plasma component can be reduced to 0 to inactivate (kill) all the retrovirus such as HIV existing in the plasma component.

Further, the present invention can be applied to the treatment of patients with renal dysfunction (renal insufficiency) by using the kidneys of xenogeneic animals in the same manner as in the treatment of hepatic dysfunction using the kidney of a different animal. Can be used as a safe, blood purification system and as a therapeutic means to prolong the life of patients with renal impairment.

[0021]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described with reference to FIGS. 1 to 3 are schematic diagrams showing a means for inactivating the virus of the present example. In the figure, 1 is a human body, 2, 4, 7
And 8 are connecting tubes, 3 is a plasma separator, 6 is an ultrasonic generator, 10 is a hepatocyte of a different animal, 16 is a material such as metal, plastics, or glass.
A coiled tube, 17 is an aqueous solution, 18 is a container, 19 is a pump, 20 is a pressure adjusting connector, and 21 and 22 are blood or plasma component inlets of whole blood. The coiled tube 16 is placed in an aqueous solution 17 inside a container 18, and the ultrasonic waves emitted by the ultrasonic generator 6 are applied to the blood in the coiled tube 16 using the aqueous solution 17 as a medium. .

FIG. 1 shows a first embodiment of the present invention, in which blood of a human body taken out of the human body 1 by the suction pressure of a pump 19 is taken out of the connecting tube 2 and the connecting tube 7. Extracorporeal circulation via When the whole blood passes through the inside of the coiled tube 16 immersed in the aqueous solution 17 in the container 18, the aqueous solution 17 is interposed from outside the outer wall of the coiled tube 16 indirectly. Ultrasonic vibration or ultrasonic irradiation is performed using the ultrasonic generator 6. As a result, the free H present in the blood without affecting the blood cells in the blood
Only the retrovirus, such as HIV, which is non-covalently associated with leukocytes on the surface of the IV, or leukocytes, is targeted to be inactivated, and then the whole blood is recirculated to the human body.

FIG. 2 shows a second embodiment of the present invention, in which the blood of a human body taken out of the body is combined with blood cells such as red blood cells, white blood cells, and platelets in the blood. Separates from plasma components, which are solutions in the blood
A plasma separator 3 is provided. The plasma component separated by the plasma separator 3 is passed through the connecting tube 5 into the coiled tube 16, and subjected to ultrasonic vibration or ultrasonic irradiation using the ultrasonic generator 6. HIV, HBV, HCV, HTLV in free state
-1, the plasma component passing through the connection tube 7 after inactivating the retrovirus such as HTLV-2, and the blood cells in the blood flowing from the plasma separator 3 through the connection tube 4 And are joined by the connection tube 8,
Reflux to the human body.

FIG. 3 shows a third embodiment of the present invention. As in the second embodiment, the blood of the human body taken out of the body is separated into blood cells and plasma components by the plasma separator 3 in the same manner as in the second embodiment. After separation, the plasma component is extracted from a xenogeneic animal, for example, a pig, and is used to pass through the inside of the hepatocyte 10 of the liver, where the hepatocyte 10 excretes waste products excreted by the human body.
For example, it decomposes excrement such as ammonia. At this time, various proteins, enzymes, hormones, amino acids, etc.,
The ultrasonic wave generated by the ultrasonic generator 6 is applied immediately before the blood component of the human body is returned to the human body by allowing the hepatic cells 10 of the pig to synthesize. Thereby, pig hepatocytes 10
Various endogenous porcine endogenous retroviruses (PERV) (retroviruses that infect humans) that are present inside and that are carried by pigs can be prevented from dissolving in the plasma components of the human body and being circulated to the human body. .

Next, an embodiment of the present invention will be described with reference to FIGS. 4 to 6 are schematic diagrams showing other means for inactivating the virus of this example. These embodiments differ from the embodiments of FIGS. 1 to 3 in that the ultrasonic generator 6 is provided with a cooling device. In fact, if ultrasonic vibrations are continuously generated in the blood and plasma components of whole blood, the temperature of the blood and plasma components of the whole blood rises, so that it is necessary to cool the blood and plasma components. Therefore, the cooling water is circulated directly to the ultrasonic vibration part of the ultrasonic generator 6 to cool it, and the temperature of the blood of the human body taken out of the body rises to about 37 ° C. or more, which is the human body temperature. Keep away from and return to the human body.

FIG. 7 shows the details of the cooling device. 3 in the figure
Numeral 0 denotes a cooling device, to which an ultrasonic generator 6 is attached via a packing 15 in a sealed state. 11 is a cooling water inlet, 12 is a cooling water outlet, 13 is a circulation path, 14 is a ceramic orifice plate, 21 is a whole blood or plasma component inlet, and 22 is a whole blood or plasma component outlet. When the whole blood or plasma component entered from the whole blood or plasma component inlet 21 passes through the circulation path 13, the whole blood or plasma component indirectly receives the ultrasonic wave from the ultrasonic transducer of the ultrasonic generator 6, Are inactivated and exit from the whole blood or plasma component outlet 22. During this time, cooling water is taken in from the cooling water inlet 11, and the vibrating portion of the ultrasonic vibrator of the ultrasonic generator 6 is directly cooled by the cooling water and discharged from the cooling water outlet 12.

The embodiment shown in FIGS. 4 to 6 will be specifically described.
In the fourth embodiment shown in FIG.
Blood of the human body taken out of the body by suction pressure is circulated outside the body from the connection tube 2 via the connection tube 7. The whole blood is sent from the whole blood inlet 21 of the cooling device 30 to the inside of the circulation path 13, and when passing through the inside of the circulation path 13, ultrasonic vibration or ultrasonic irradiation is performed by the ultrasonic generator 6. , So that the vibrating part of the ultrasonic transducer does not directly contact the blood of the whole blood,
Ultrasonic vibration is applied during the passage through the inside of No.3. This allows free HIV present in the blood or HIV non-covalently bound to leukocytes on the surface of leukocytes without affecting blood cells in the blood.
After inactivating only the retrovirus such as the target, the whole blood is circulated to the human body from the whole blood outlet 22 through the connection tube 7. At this time, cooling water is supplied from the cooling water inlet 11 so that the whole blood has a temperature of 3
The temperature should not rise above 7 ° C. If the vibrating portion of the ultrasonic transducer of the ultrasonic generator 6 and the blood or plasma components of whole blood are in direct contact with each other, no matter how much cooling is performed from the outer periphery, cooling is not possible. A problem arises in that blood or plasma components are denatured.

FIG. 5 shows a fifth embodiment of the present invention, in which the blood of the human body taken out of the body is extracted from the blood of the human body, such as red blood cells, white blood cells, and platelets, which are cells in the blood. A plasma separator 3, which separates into a solution, a plasma component, is provided. The plasma component separated by the plasma separator 3 is sent from the plasma component inlet 21 of the cooling device 30 to the inside of the circulation path 13 by the connection tube 5,
When passing through the inside of the device, ultrasonic vibration or ultrasonic irradiation is performed using the ultrasonic generator 6, and the free HIV, HBV, HCV, HTLV-
1. After inactivating retroviruses such as HTLV-2, the plasma components passing through the connection tube 7 and the blood cells in the blood flowing from the plasma separator 3 through the connection tube 4 are separated from each other. Then, they are joined by the connecting tube 8 and recirculated to the human body. Also in this embodiment, the cooling water inlet 11
To supply the cooling water to prevent the whole blood from coming into direct contact with the vibrating portion of the ultrasonic vibrator of the ultrasonic generator 6 so that the temperature of the human body becomes about 37 ° C. or higher. Avoid temperature rise.

FIG. 6 shows a sixth embodiment. In the same manner as in the fifth embodiment, after the human blood taken out of the body is separated into blood cells and plasma components by the plasma separator 3, the plasma The component is removed from a heterologous animal, for example, a pig, and is passed through the inside of a hepatocyte 10 of the liver, which is used to decompose waste products excreted by the human body, such as ammonia, by the hepatocyte 10. Let it. At this time, various proteins, enzymes, hormones, amino acids, etc. are transferred to pig hepatocytes 10
The ultrasonic wave generated by the ultrasonic generator 6 is applied immediately before the blood plasma component of the human body is returned to the human body. Thereby, it is present in the porcine hepatocytes 10.
Various endogenous porcine retroviruses (PERVs) (retroviruses that infect humans), which are carried by pigs, can be prevented from dissolving and dissolving in plasma components of the human body and being circulated to the human body. Also in the present embodiment, the cooling water is supplied from the cooling water inlet 11 and the cooling water is brought into direct contact with the vibration portion of the ultrasonic vibrator of the ultrasonic generator 6 to directly cool the vibration portion. Then, the temperature of whole blood is prevented from rising above 37 ° C., which is the body temperature of the human body.

FIGS. 8 and 9 show another example of the coiled tube of the present invention. In the embodiment of FIGS. 1 to 3, the tube is formed in a spiral shape. However, as shown in FIG. 8, by forming the tube 16 in a spiral shape, the ultrasonic wave generated by the ultrasonic generator 6 having a strong directivity can be obtained. Irradiation can be performed efficiently.

The plasma separator 3 shown in FIGS. 2, 3, 5 and 6 is manufactured by Asahi Medical Co., Ltd. under the trade name of Plasma Flow (model number OP-
05W), plasma components can be easily separated. In addition, as a means for separating the plasma component, a blood cell and a plasma component may be separated by using a means such as a centrifuge. Alternatively, other separation means may be used.

As a means for continuously treating the virus in the ultrasonic generator 6 and the plasma components, the use of a Sony Fire manufactured by Branson of the United States and a continuous crushing adapter makes it easier to use the whole. Structure can be assembled.

In the above embodiment, one ultrasonic generator 6 is used, but a plurality of ultrasonic generators 6 arranged in series or in parallel may be used. However, the continuous crushing adapter manufactured by Branson was used in a manner different from the usage manual of the continuous crushing adapter. In the manual, a circulation path 13 through which cooling water is circulated for the purpose of cooling the outer periphery (outside) of the main body of the continuous crushing adapter by passing cooling water through
The purpose of this treatment is to pass a solution such as whole blood or plasma components through the inside of the Branson, and use it in a manner different from the Branson manual. in this case,
Cooling water is circulated through the circulation path 13 for passing a solution such as blood or plasma components of whole blood, and the vibrating part of the ultrasonic generator 6 is brought into direct contact with the cooling water using the cooling water. Then, the vibrating part is cooled directly, so that the vibrating part of the ultrasonic generator 6 and the solution such as whole blood or plasma components do not directly contact the vibrating part. For the same purpose as above, glass, plastics, metal,
Or other material, the coiled tube 16
Is placed in an aqueous solution, and the aqueous solution is interposed, and cavitation by ultrasonic vibration is given to a solution of whole blood, blood, or a plasma component while passing through the inside of the coiled tube 16; The structure is intended to prevent the temperature of a solution of whole blood, such as blood or a plasma component, from increasing by indirectly giving the solution to blood, plasma, or the like.

[0034]

As described above, according to the present invention, the HI
Extend the life of patients infected with a retrovirus, such as V, HBV, HCV, HTLV-1, HTLV-2, or PERV, or patients with liver dysfunction (liver failure) or kidney dysfunction (renal failure) And a method for producing a safe blood product. US Dr. Stephan R. As
h is ASAIO Journal April 199
The body temperature of the human body announced in 7 years, around 42 ℃,
When raised and maintained for several hours, retroviruses such as HIV, which are non-covalently bound to the surface of lymph nodes and leukocytes, are shed, leaving lymph nodes and leukocytes in the blood of the human body. Retroviruses, such as HIV, detached from the surface dissolve in the blood. However, by raising the temperature of the human body to around 42 ° C., it is possible to dissolve retroviruses such as HIV in the blood of the human body, but it is not possible to inactivate (kill) retroviruses such as HIV. Can not. This heat treatment means,
The combined use of the ultrasonic vibration or the cavitation treatment means according to Bernoulli's law makes the treatment means more effective.

[Brief description of the drawings]

FIG. 1 is an explanatory diagram showing a first embodiment of the present invention.

FIG. 2 is an explanatory diagram showing a second embodiment of the present invention.

FIG. 3 is an explanatory view showing a third embodiment of the present invention.

FIG. 4 is an explanatory view showing a fourth embodiment of the present invention.

FIG. 5 is an explanatory view showing a fifth embodiment of the present invention.

FIG. 6 is an explanatory view showing a sixth embodiment of the present invention.

FIG. 7 is a sectional view showing an example of a cooling device of the ultrasonic generator according to the present invention.

FIG. 8 is a plan view showing another example of the coiled tube of the present invention.

FIG. 9 is a sectional view showing another example of the coiled tube of the present invention.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Human body 2,4,5,7,8 Connection tube 3 Plasma separation device 6 Ultrasonic generator 10 Heterogeneous animal liver 11 and 12 Cooling water inlet / outlet 13 Circulation path 14 Ceramic orifice plate 15 O-ring 16 Coiled tube 17 Aqueous solution 18 Container 19 Pump 20 Connector for pressure regulation 21 Whole blood or plasma component inlet 22 Whole blood or plasma component outlet 30 Cooling device

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A61M 1/02 500 A61M 1/02 500

Claims (9)

[Claims] 1. A connection tube and a pump for drawing blood from a human body and circulating the blood into the body of the human body, and red blood cells, which are cells in the blood, to whole blood flowing through the connection tube.
An ultrasonic generator that performs ultrasonic vibration or ultrasonic irradiation with an intensity within a range that does not destroy cells such as white blood cells and platelets, and an ultrasonic vibration or ultrasonic irradiation by the ultrasonic generator, HIV, HB present in blood
V, HCV, HTLV-1, HTLV-2, or other retroviruses are inactivated, and then the whole blood is returned to the human body to inactivate the virus. 2. A connection tube and a pump for drawing blood from a human body and circulating into the body of the human body, cells such as red blood cells, white blood cells, platelets and the like, which are cells in whole blood blood drawn from the human body, and plasma. A plasma separator that separates the components into components, an ultrasonic generator that performs ultrasonic vibration or ultrasonic irradiation on the separated plasma components within a range that does not destroy the plasma components, and a plasma component after the ultrasonic irradiation. And means for merging the cells in the blood with the ultrasonic wave generated by the ultrasonic generator or the ultrasonic irradiation, whereby HIV, HBV, HCV, HTLV-1, and HTL present in the plasma component are provided.
After inactivating V-2 or other retroviruses,
A device that inactivates viruses that allows blood to flow back to the human body. 3. A connection tube and a pump which draws blood from a human body and circulates into the human body, and separates cells such as red blood cells, white blood cells, platelets and the like, which are cells in the blood drawn from the human body, into plasma components. A plasma separator, a means for decomposing waste components present in human plasma components through the inside of the hepatocytes of a living liver of a different animal, and a strength within a range that does not destroy the plasma components. An ultrasonic generator that performs ultrasonic vibration or ultrasonic irradiation, and a plasma component after the ultrasonic irradiation, and a unit that merges the cells in the blood is provided, and the ultrasonic vibration by the ultrasonic generator, or HIV, H present in plasma components by ultrasonic irradiation
A device for inactivating a virus, which inactivates BV, HCV, HTLV-1, HTLV-2, or other retroviruses and then returns blood to the human body. 4. A variety of proteins, which are added to live liver hepatocytes.
4. The apparatus for inactivating a virus according to claim 3, wherein the apparatus synthesizes enzymes, hormones, amino acids and the like. 5. The virus according to claim 1, further comprising a cooling device that suppresses an increase in blood temperature caused by ultrasonic vibration or ultrasonic irradiation by an ultrasonic generator. Device to inactivate. 6. The method according to claim 6, wherein the temperature of the blood is the body temperature of the human body.
6. The apparatus for inactivating a virus according to claim 5, wherein the apparatus is cooled so as to keep the temperature at about ° C. 7. The virus according to claim 1, wherein a plurality of ultrasonic generators arranged in series or in parallel are used to inactivate a retrovirus such as HIV in a plasma component. Device to inactivate. 8. A plasma component is extracted from blood extracted from a human body and subjected to ultrasonic vibration or ultrasonic irradiation having an intensity within a range that does not affect proteins, enzymes, hormones, amino acids and the like in the plasma component. , HIV and HB in plasma components
V, HCV, HTLV-1, HTLV-2, or other retrovirus or parvovirus,
A method for producing a blood product, which comprises producing a safe blood product. 9. When ultrasonic vibration is applied to the blood or plasma component of whole blood, the vibrating portion of the ultrasonic vibrator of the ultrasonic generator directly contacts the blood or plasma component of the whole blood. Without inducing, between the vibrating part and the blood or plasma component of whole blood, a solution such as a cooled aqueous solution is interposed between the vibrating part and the blood or plasma component of whole blood, and the ultrasonic wave is indirectly introduced into the blood or plasma component of whole blood. A device for inactivating (killing) a retrovirus such as HIV, which is present in the blood of whole blood or in a plasma component, by cavitation according to (1).