JPH07155177A - Method for deactivating virus - Google Patents

Abstract

PURPOSE:To kill or deaetivate microbes such as bacteria or virus existing in the blood, plasma components and other solutions. CONSTITUTION:The blood is taken out of the body and subjected to a centrifugal separator 5 to separate it into cells such as erythrocyte, leukocyte and blood platelet and plasma components to transfer the virus in the blood into the plasma components, which are then fed, via a connecting tube 6, to a vessel 1. Using an ultrasonic wave oscillating section 3 set up inside the vessel 1, ultrasonic waves are given into the plasma components to treat them physically and deactivate the virus in the plasma components. The resultant plasma components and a solution containing the aforementioned cells separated by the centrifugal separator 5 are fed, via connecting tubes 7 and 8, into a mixer 9, respectively, where the plasma components and the cells are put together.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a technique necessary for sterilizing and inactivating microorganisms such as bacteria and viruses existing in blood, plasma components and other solutions.

[0002]

2. Description of the Related Art Conventionally, as a method for inactivating and suppressing viruses, vaccines and drugs have been used to inactivate and suppress viruses, but viruses such as HIV virus, hepatitis virus and carcinogenic virus have been used. However, at present, no effective treatment method has been established.

[0003]

It is an object of the present invention to kill and inactivate microorganisms such as bacteria and viruses existing in blood, plasma components and other solutions.

[0004]

FIG. 1 is an explanatory view of the principle of the present invention. FIG. 1 is a longitudinal sectional view, in which 1 is a container made of glass or plastic and 2 is an ultrasonic wave. In the apparatus, 3 is an oscillating portion that oscillates ultrasonic waves, and 4 is a joining portion made of rubber.

[0005]

When the blood of the human body is drawn out of the body and the blood circulating in the body is separated into cells such as red blood cells, white blood cells and platelets and plasma components using the centrifuge 5, for example, blood Since the free HIV virus present therein has a very small size of 200 nm, the majority of the free HIV virus present in blood will be transferred to the plasma component by separating the blood.

Since important cells in blood, such as red blood cells, white blood cells and platelets, are vulnerable to physical pressure waves of ultrasonic waves and are quickly destroyed, ultrasonic waves should be applied to all blood. Since it has a problem with erythrocytes, it is present in blood after being separated into cells such as red blood cells, white blood cells and platelets, which are weak against ultrasonic waves, and protein components such as liquid proteins, which are relatively strong against ultrasonic waves, and plasma components. The majority of the HIV virus in the free state is moved into the plasma component, and the HIV virus in the free state is moved from the blood, without destroying the plasma component by applying ultrasonic waves to the plasma component, Using the physical cavity phenomenon of ultrasonic waves, the HIV virus is destroyed in the envelope part, which is one part of HIV in a free state, or the entire HIV virus, which is present in plasma components. The use of the phenomenon that can be inactivated.

Plasma components after inactivation of free HIV virus present in plasma components using ultrasonic waves and removal of HIV viruses present in plasma components,
Cells such as erythrocytes, leukocytes, and platelets separated by the centrifuge 5 are combined and put together and refluxed to the human body without damaging erythrocytes, leukocytes, and platelets that are vulnerable to the physical cavity phenomenon of ultrasonic waves. , It can reduce the number of HIV virus in the blood.

Non-covalent binding of leukocytes, etc. on the surface by utilizing the physical destructive force of ultrasonic waves to the extent that important cells in blood, such as erythrocytes, leukocytes and platelets, are not destroyed. Cavity phenomenon, which is the destructive force of the ultrasonic physical pressure wave, is caused by selectively resonating only viruses such as HIV virus and non-covalently binding on the surface of infected cells. , The cells are forcibly shaken, separated from the surface of the infected cells, separated into the blood and lysed, and then the blood is centrifuged to remove red blood cells,
Separation into cells such as white blood cells and platelets and plasma components, transfer of viruses such as HIV virus in blood to plasma components,
The HI in the plasma component is treated by applying ultrasonic waves to the plasma component in which a virus such as HIV virus is dissolved
By inactivating the V virus, the virus such as the HIV virus that is non-covalently bound to the surface of the cell is indirectly bonded without damaging the cells such as red blood cells, white blood cells, and platelets, which are weak against ultrasonic destructive power. It can be inactivated.

[0009]

EXAMPLE An example of the present invention will be described below with reference to FIG. FIG. 2 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 2 is a vertical cross-sectional view.
1 is a container made of glass or plastic or the like, 2 is a device for oscillating ultrasonic waves, 3 is an oscillating part for oscillating ultrasonic waves, 4 is a joining part made of rubber or the like, and 5 is a centrifuge. , 6, 7 and 8 are connecting pipes.

FIG. 2 shows that the blood of the human body is drawn out of the body, and the blood drawn out of the body is subjected to a centrifuge 5 to collect red blood cells, white blood cells and white blood cells, which are components in the blood. When separated into platelets and plasma components, the HIV virus has a very small diameter of 200 nm, so it moves into the plasma components. It is transferred into the plasma components using the centrifuge 5, and the HIV virus is contaminated. The plasma component present in the container 1 is sent to the inside of the container 1 using the connecting pipe 6, and the ultrasonic wave oscillating portion 3 provided inside the container 1 is used to convert the plasma component into the inside of the container 1. A sound wave is continuously applied to physically destroy a part or all of the HIV virus present in the plasma component, inactivate the HIV virus in the plasma component, and physically destroy the HIV virus in the plasma component. After removing the plasma component, The solution containing the cells such as red blood cells, white blood cells and platelets separated by the centrifuge 5 in the mixer 9 is sent from the centrifuge 5 to the mixer 9 using the tube 8. It is sent to the mixer 9 via the connecting pipe 7 to inactivate the HIV virus in the plasma component,
The plasma component obtained by removing the HIV virus from the plasma component is merged in the inside of the mixer 9 to be combined, and then the plasma component is refluxed to the human body.

[0011]

EFFECTS OF THE INVENTION Blood is drawn out of the body, and the blood drawn out of the body is subjected to a centrifuge, and cells such as red blood cells, white blood cells and platelets, which are weak against the physical destructive force of ultrasonic waves, and ultrasonic physical waves. Centrifugal separation is used to separate the plasma components that are highly destructive, and the majority of free HIV viruses present in the blood are transferred to the inside of the plasma components, and the HIV viruses that have migrated to the plasma components are separated. After inactivating the HIV virus present in the plasma component using ultrasonic waves without damaging the plasma component, cells such as red blood cells, white blood cells and platelets separated by a centrifuge and the plasma component are separated. By merging, bringing together and returning to the human body, the HIV virus in the free state existing in the blood is damaged without damaging cells such as red blood cells, white blood cells and platelets, which are weak against the physical destructive power of ultrasonic waves. By that can be inactivated,
By lowering the HIV virus level in the blood, it is possible to delay the onset of AIDS, which is effective as a therapeutic means.

[0012] The therapeutic means of the present invention is designed so that the HI
Although it is not possible to completely eradicate viruses such as V virus, HI is used by using the physical destructive power of ultrasonic waves.
Since a virus such as V virus is destroyed, any virus other than HIV virus can be inactivated, and thus the therapeutic means of the present invention can be used as a therapeutic means for all viral diseases.
Since the therapeutic means of the present invention causes no adverse effects such as side effects at all, it has an effect that it can be used as a therapeutic means that can be applied to various and various viral diseases.

[Brief description of drawings]

FIG. 1 is an explanatory view of the principle of the present invention, and FIG. 1 is a vertical sectional view.

FIG. 2 is a schematic structural explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 2 is a vertical sectional view.

[Explanation of symbols]

1. A container made of glass or plastic (abbreviated as
Make a container) 2. Device that oscillates ultrasonic waves 3. 3. Oscillation part that oscillates ultrasonic waves (abbreviated as oscillation part) 4. Joint part made of rubber etc. 5. Centrifuge 6.7 and 8. Connecting pipe

Claims (5)

[Claims] 1. HI present in blood or plasma components
In order to inactivate viruses such as V virus, ultrasonic treatment is applied to blood or plasma components, and viruses existing in blood or plasma components are utilized by utilizing the destructive force of physical pressure waves of ultrasonic waves. How to inactivate. 2. A method of inducing blood out of the human body and inactivating blood or plasma components circulating in the body by applying ultrasonic waves to inactivate viruses such as HIV virus in the blood or plasma components. (1) A method for inactivating the virus according to (1). 3. The HIV virus or the like existing in the blood is drawn out of the human body, and the blood is centrifuged to separate it into cells such as red blood cells, white blood cells and platelets and plasma components. Virus in the blood plasma component,
Connect the plasma component in which the virus in the blood is transferred to the connecting pipe (6)
Is sent to the container (1), and the ultrasonic wave oscillating portion (3) provided inside the container (1) is used to oscillate ultrasonic waves into the plasma component for physical treatment. A red blood cell separated by a centrifuge (5) from a plasma component in which the virus in the plasma component has been inactivated and the virus in the plasma component has been inactivated,
A solution containing cells such as white blood cells and platelets is mixed using a connecting pipe (7) and (8), respectively, into a mixer (9).
The plasma component in which the virus present in the plasma component is inactivated and the cells such as red blood cells, white blood cells and platelets are merged and mixed in the mixer (9) and then refluxed to the human body. A method for inactivating the virus according to 1) or 2). 4. Leukocytes are obtained by treating the blood by irradiating the blood outside the body of the human body with ultrasonic waves to the extent that the cells such as red blood cells, white blood cells and plasma plates are not destroyed in the extracorporeally circulating blood. (1), wherein the virus such as HIV virus which is non-covalently bound to the cell on the surface of the cell such as the above is detached or shaken from the surface of the cell such as the white blood cell and the like to be released into the blood. A method for inactivating the virus according to 2) and 3). 5. Leukocytes are obtained by treating the blood by applying ultrasonic waves within a range that does not destroy cells such as red blood cells, white blood cells and platelets in the blood that is circulated outside the human body by circulating the blood outside the human body. After separating or shaking the virus, such as HIV virus, which is non-covalently bound to the cells, such as white blood cells, from the surface of the cells, such as white blood cells, and separating them into the blood, centrifuge the blood. In a vessel (5), the cells and plasma components are separated, and the plasma components are sonicated to inactivate viruses such as HIV virus in the plasma components,
A method for inactivating the virus according to any one of claims (1), (2), (3) and (4) which is refluxed to the human body.