SU1386169A1 - Method of separating the eggs of opistorchis - Google Patents

Abstract

This invention relates to medicine and veterinary medicine, more specifically to parasitology. Intended for the preparation of antigenic preparations and the production of immune sera. The purpose of the invention is to increase the yield and simplify the method by destroying the bodies of opistorhas suspended in 5-25 ml of 0.85% NaCl solution. Ultrasonic disintegration is carried out using an exponential radiator at a frequency of 44 MHz, power 65–70 W / cm, treatment time 10–20 min, suspension concentration 1.2 opistorch in 1 ml. The number of simultaneously treated opistorch 80-100 pcs. The helminth bodies are destroyed completely, the integrity of the eggs is maintained. 1 tab. WITH

Description

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The invention relates to medicine and veterinary medicine, in particular to parasitology, and can be used in the preparation of antigenic preparations and the production of immune serums.

The aim of the invention is to increase the yield and simplify the method.

The goal is achieved by destroying the bodies of opistorchs; the viscosity of various modes of disintegrating in 5–25 ml of 0.85% is estimated by the minimum required solution of sodium chloride, ultrasonic processing time, and by final disintegration using exponential emitter at a frequency of 44 kHz, power 65-70 W / cm, processing time 10-20 minutes, concentration of suspension-1.2 opistorch in 1 ml The number of opistorhas simultaneously processed 80-100 pcs. In this case the helminth bodies are completely destroyed, but the integrity of the eggs is preserved.

Example 1. 100 opistorchs in 50 ml of a 0.85% sodium chloride solution were ultrasonically disinfected using a UZDN-1 device in operation: frequency 44 kHz, power 70 W / cm, treatment time 20 minutes. Ultrasonic treatment of helminths in this mode ensured complete destruction of the bodies of parasites, but retained the integrity of the opistorha's hens. After dysenteration, the suspension of opistorch eggs was transferred to test tubes and centrifuged at 1000 rpm by destroying the bodies of opistorhs, about t for 3 minutes. The precipitate, consisting of whole eggs, was washed three times with a 0.85% sodium chloride solution with an unspecified pH by centrifugation at 1000 rpm for 2-3 minutes. The obtained opisthorch eggs were stored in a 0.85% solution of sodium chloride at -20 ° C.

At the same time, out of 100 opistorhis, the eggs were isolated by a known method.

The purity of the preparations of eggs was assessed by the presence of parts of the opisthorch bodies, established microscopically. The data characterizing the proposed known methods are shown in the table.

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44 kHz (the frequency values are determined by the fixed characteristics of the domestic disperser UZDN-1, where 44 kHz is the maximum frequency), power 50, 60, 65, 70 and 80 W / cm, duration 10, 20 and 30 min, volume 5.25 and 50 ml and suspension concentrations of opistorhas 1.2 and 3 in 1 ml. Effectiveness of the preparation of eggs and integrity of eggs.

Taking into account the expediency of ensuring the shortest processing time, the largest volume and concentration of opistorch suspension, the following disintegration parameters are optimal: frequency 44 kHz, power 65-70 W / cm, treatment time 20 min, suspension volume 5-25 ml, concentration 1 -2 oyistorhav ml.

Thus, the use of a simple method of ultrasonic disintegration of opisthorch bodies in the selected mode provides for a relatively short time and with a very small amount of work, the production of relatively large quantities of helminth eggs. At the same time, the yield of eggs from the same number of opistorchs almost doubled. Invention Form

Method for isolating opistorch's eggs

characterized in that, in order to increase the yield and simplify the process, the destruction is carried out using ultrasonic disintegration, while a suspension of opisthorses in the amount of 5-25 ml suspended in a 0.85% solution of sodium chloride at a concentration of 1-2 operistor in 1 ml, sonicate at a frequency of 44 kHz and a power of 65-70 W / cm for 10-20 minutes.

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The various disintegration modes were estimated by the minimum required processing time, purely 44 kHz (frequency values are determined by the fixed characteristics of the domestic dispenser UZDN-1, where 44 kHz is the maximum frequency), power 50, 60, 65, 70 and 80 W / cm, duration 10, 20 and 30 min, the volume of 5.25 and 50 ml and the concentration of suspension opistorch 1,2 and 3 in 1 ml. Effective

the various modes of disintegration were estimated by the minimum required duration of treatment, purely by the destruction of the opistorha bodies, o

those of the preparation of eggs and the integrity of the eggs.

Taking into account the expediency of ensuring the shortest processing time, the largest volume and concentration of opistorch suspension, the following disintegration parameters are optimal: frequency 44 kHz, power 65-70 W / cm, treatment time 20 min, suspension volume 5-25 ml, concentration 1 -2 oyistorhav ml.

Thus, the use of a simple method of ultrasonic disintegration of opisthorch bodies in the selected mode provides for a relatively short time and with a very small amount of work, the production of relatively large quantities of helminth eggs. At the same time, the yield of eggs from the same number of opistorchs almost doubled. Invention Form

Method for isolating opistorch's eggs

by destroying the bodies of opistorchus, oh

characterized in that, in order to increase the yield and simplify the process, the destruction is carried out using ultrasonic disintegration, while a suspension of opisthorses in the amount of 5-25 ml suspended in a 0.85% solution of sodium chloride at a concentration of 1-2 operistor in 1 ml, sonicate at a frequency of 44 kHz and a power of 65-70 W / cm for 10-20 minutes.

Claims (1)

The invention. The method of isolation of opisthorchis eggs by destroying the bodies of opisthorchias, characterized in that, in order to increase the yield and simplify the method, the destruction is carried out using ultrasonic disintegration, while the suspension of opisthorchia in the amount of 5-25 mp, suspended in 0.85% solution of sodium chloride at a concentration of 1-2 opisthorch in 1 ml, is treated with ultrasound at a frequency of 44 kHz and a power of 65-70 W / cm ² for 10-20 minutes. Performance indicators Isolation time, min. Number of eggs isolated Egg purity Way Famous 300-350 1080000 The drug is clean Proposed 2,100,000 The drug is clean