JP2000032870A - Stress reaction suppressant, transportation of livign fish using the same and the living fish - Google Patents

Abstract

PROBLEM TO BE SOLVED: To transport fishery organisms such as fishes healthily using a stress reaction suppressant because their stress reaction to various stressors can be mitigated or removed by the aid of the above suppressant. SOLUTION: When fishery organisms such as fishes are to be transported alive, a stress reaction suppressor consisting mainly of seashell fossil as a crystal body with humus solubility consisting of calcareous and/or siliceous matter where various kinds of nectons, planktons, sea weeds including sea algae, etc., are embedded and accumulated, is added to water so as to get cloudy, and in this state, the fishery organisms are transported; thereby, the cloudy state mitigates the stress of the organisms caused by highly dense containment and light, the stress reaction suppressor dispersed in the water adsorbes harmful substances derived from the excreta of the organisms and absorbes carbon dioxide, and prevents the water from pH level lowering, resulting in mitigating and suppressing the stress reaction otherwise developed in the organisms, therefore enabling them to be transported healthily with good body color and liveliness.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to a stress response inhibitor for use in transporting marine animals including shrimp, crab, octopus, squid, shellfish, turtle, etc. while keeping saltwater fish and freshwater fish alive. The present invention relates to a method of transporting live fish using the same and the live fish.

[0002]

2. Description of the Related Art Live fish refers to live and healthy fish, not merely to live fish. Live fish transport refers to live and healthy fish (such as shrimp, crab, shellfish, etc.). (Including animals), transporting them as is, and delivering them to the end user. Therefore, various measures have been devised for transporting live fish.
From the simplest method of transporting live fish to transporting live fish to vehicles, the minimum requirement is that live fish be alive until consumed, and the main factor in the death of live fish is Some vehicles are provided with aeration equipment because of lack of oxygen.

[0003] The marine animals to be transported are subjected to various stimuli such as handling, forced movement, high-density storage, vibration and light, a decrease in the amount of dissolved oxygen in water in the transport container, and deterioration in water quality due to excrement. Can be These stimuli act as stressors on aquatic animals, causing various physiological responses,
Causes stress response. According to Hiroshi Ishioka, Fisheries Science Series No. 39, “Live Fish Transport”, Koseisha Koseikaku (1982), this stress response is a temporary change in the release of hormones into the blood. Metabolic disturbances include metabolic disturbances, osmoregulatory disturbances, changes in various reactions under autonomic innervation, changes in blood properties, and changes in mucus secretion. Therefore, during transport, the marine animal undergoes one of the above-mentioned stress responses, and the stress response varies depending on the type and intensity of the stimulus given, the condition of the marine animal before the stimulus, the water temperature, etc. Different styles and degrees. This leads to a case where the transportation is successful and a case where the transportation is not successful even if the transportation conditions that can be easily controlled artificially, such as the accommodation amount of the live fish and the ventilation amount of the aeration, are the same.

[0004] Under such circumstances, there is a method of transporting live fish while keeping the water temperature in the transport container low while paying attention to the fact that marine animals are metamorphic animals. This is advantageous because it can eliminate many negative factors, such as reducing exercise, reducing metabolism and excretion, reducing oxygen consumption, and increasing the binding strength between hemoglobin and oxygen. Specifically, the water in the transport container is cooled in advance, and a heat insulating material,
There are a method of preventing the temperature rise during transportation by making full use of the shade coating, and a method of mounting a cooling device on the vehicle. The method of cooling the water in the transport container in advance is to install a circulation cooling tank with a septic tank at a relay base near the quay, for example,
When the temperature of the outside seawater is 26 ° C., the seawater in the circulation cooling tank is set to about 15 ° C., and the seawater in the circulation cooling tank and the outside seawater are placed in a transport container on the vehicle at a ratio of 1: 1. The temperature is about 4 ° C. lower than the temperature of the external sea water, that is, about 4 ° C. lower than the body temperature of the marine animal. After that, the marine animals are housed in the shipping container, the seawater in the shipping container is circulated with the seawater at 15 ° C in the circulating cooling tank and gradually lowered to around 15 ° C, and then the live fish is transported. At this time, the heat insulation and the sunshade coating are fully utilized to prevent the temperature rise of the seawater in the shipping container and eliminate many negative factors. It is a matter of course that the method of mounting a cooling device on a vehicle makes full use of heat insulating materials and sunshade painting.However, since it is possible to cool the amount of heat that enters from the outside during transportation, it is possible to use marine animals that are greatly affected by temperature. This is convenient in cases and long-distance transportation.

[0005] There is also a method of improving the condition of marine animals so that the health of the marine animals before transportation is adjusted and the harsh conditions of transportation can be tolerated. For example, the supply of oxygen tends to be inadequate during transportation.However, by adapting marine animals in advance to withstand hypoxia, or adding phospholipids to feed and giving them to marine animals, the stress tolerance of marine animals can be reduced. This is done by raising the diet or controlling the feeding so that iron and vitamins are not deficient so that marine animals are less likely to develop anemia.

[0006]

However, in the above-mentioned method, although the minimum necessary point that the live fish are alive, the decrease in the amount of dissolved oxygen in water in the transport container and the lack thereof are cleared, the above-mentioned problems can be solved. Various stressors such as density containment, water quality deterioration due to vibration, light and excrement cannot be mitigated or eliminated, and it is difficult to hand over to the final user in a healthy fish state. In addition, utilizing the fact that the aquatic animal is a thermophilic animal, it is convenient that the temperature of the water in the transport container can be kept low to slow down the activity and eliminate many negative factors.
However, high-density housing, the stress caused by vibration and light cannot be removed, and the activity of marine animals slows down, and although the degree of water quality deterioration due to excrement decreases, it does not improve, and circulation with a septic tank is not possible. Extensive equipment is required, such as installing a cooling tank and installing a cooling device in the vehicle. Furthermore, methods for improving the constitution of marine animals so as to withstand various stressors are known only in a fragmentary manner to the extent described above, and the types of marine animals, individuals It is not clear how the degree of improvement in constitution changes depending on differences and water temperature.

Therefore, the present invention has been made in view of the above circumstances, and requires little installation of large-scale equipment and modification of existing equipment, and is capable of high-density storage, light,
It can reduce and eliminate stress response of marine animals to various stressors such as deterioration of water quality due to excretion, transport truly lively and healthy marine animals as they are, It is an object of the present invention to provide a stress reaction inhibitor which can be delivered to an animal, a method for transporting live fish using the same, and a live fish thereof.

[0008]

The present inventor has been investigating and studying the composition and properties of shell fossils for many years. In addition, we have continued research on fish farming and the maintenance and management of water quality and bottom sediment in fish farms, and have achieved certain results.
The water quality and bottom quality of the aquaculture fishing grounds have been successfully improved, and it has become possible to produce lively and healthy fish, that is, live fish. This time, on an extension of this, we continue to study vigorously how the live fish successfully produced can be transported while maintaining the same condition and delivered to the final user. I came. As a result, shellfish fossils are added and mixed in the transport container that contains live fish to make it cloudy, and when transported in that state, the cloudy state reduces the stress caused by high-density storage and light, and shellfish fossils dispersed in water excrete fish. The present inventors have found that they can adsorb harmful substances due to substances, absorb carbon dioxide due to the respiratory action of fish, prevent a decrease in pH, and can transport fish with good color, vitality and healthy fish, and have reached the present invention.

That is, the first aspect of the present invention comprises, as a main component, shell fossil, which is a humus-soluble crystalline body in which various nekton, plankton, algae, seaweed, and the like made of calcareous or silicic acid are buried and deposited. And a stress response inhibitor characterized by relaxing and suppressing a stress response generated in a marine animal when the marine animal is transported alive.

The shell fossils used in the present invention are foraminiferal fossils in the archaeological name and calcareous sandstone in the geological name. It is produced in prefectures, Fukushima prefecture and Kagoshima prefecture, but there is no limitation depending on the place of production. Shell fossils from any locality may be used as long as the shell fossils have the following characteristics. Table 1 shows the analytical values of shell fossils in the main production areas.

More specifically, the shell fossils of the present invention are based on the following quantitative analysis table (Table 2) of samples mined at several mines in Toyama Prefecture, and mined from these mines. An analog of the fossil shell of the ingredients shown in Table 2,
It is.

[0012]

[Table 1]

[0013]

[Table 2]

The fossil shells mined in Toyama Prefecture differ greatly in the molecular composition from those of shell fossils mined in other parts of Japan, and contain a certain amount of silicon. Is characterized by a very high content of. In addition, this fossil shell has an aragolite-type crystal structure secreted from living organisms, and has a myriad of small holes having a certain effective diameter, and these numerous small holes do not include those containing crystal water. There are things, too.
These pores that do not contain water of crystallization have adsorption performance similar to activated carbon, and may exhibit tens of times the capacity of activated carbon depending on the type of the substance to be adsorbed. It is within the scope of the present invention to use shell fossils, which are the main components of the stress response suppressor, together with other substances, such as enzymes, vitamins, etc., to achieve a certain effect.

The marine animals include, but are not limited to, those to which live fish are currently transported at the time of filing the present invention. For example, among seawater inhabitants, red sea bream, black porgy, ishidai, ishigakidai, amberjack, Horse mackerel,
Japanese mackerel, flounder, flounder, sea bass, tiger puffer, tiger puffer, riverfish, quailfish, squid, grouper, onion, crocodile, scallop, spider, lobster, prawn, shrimp, crayfish, scallop, octopus, squid, abalone, turtle, scallops, scallops, etc. Inhabiting animals include eels, trout, carp, sweetfish, loach, sculpin, pehelei, and turtle.

The stress response includes any of the above-mentioned stress reactions that occur when a marine animal is transported alive.
As described above, this stress response is, as a primary change, a release of hormone into the blood, and as a result, a secondary change is a metabolic disturbance, a disturbance in osmoregulatory function, and various responses under autonomic innervation. Changes, changes in blood properties, changes in mucus secretion, and the like. Here, in the case of farmed fish, transportation includes everything from the farm to delivery to the final user, and therefore also includes storage by land ikesu. For natural fish, this includes everything from offshore processing to delivery to the final user. In this case, if there is stockpiled by land-based ikesu, it is naturally included.

The use form of the stress response inhibitor containing shell fossils as a main component is as follows. Shell fossils are added to a transport container containing a marine animal, and mixed and turbid using a stirrer and aeration equipment. It depends. It is desirable that the shell fossils be crushed and powdered in order to make the shell fossils more turbid, but there is no problem even if they are in a lump state. On the other hand, the turbid shell fossils charged into the transport container disperse and become cloudy, reducing stress on marine animals due to high-density storage and light. Shell fossils dispersed in water
It absorbs harmful substances from the excrement of marine animals, absorbs carbon dioxide by respiratory action, prevents the pH from lowering, and alleviates and suppresses many stress reactions except for stressors on marine animals. This was apparent in the cortisol, hemoclobin, and hematocrit values, which are indexes reflecting the stress response of marine animals, and was particularly apparent in the cortisol amount.

According to a second aspect of the present invention, the fossil shellfish includes various nektons, planktons, algae, and the like made of calcareous or silicic acid.
A humus-soluble crystal is deposited when seaweeds are buried and deposited.
It is a stress reaction inhibitor that is obtained by heat treatment in the range of 50 ° C. to 300 ° C. to remove and activate water of crystallization.

The heat treatment of the shell fossil in the range of 150 ° C. to 300 ° C. means that the crystallization water contained in the pores is removed, the adsorption performance is improved, and the shell is adhered to the shell fossil by the heat treatment. This is for killing various bacteria. Therefore, when the heat-treated shell fossil is used as a main component, the adsorption performance is increased and the performance as a stress reaction inhibitor is increased by the amount of water of crystallization contained in the small holes.

According to a third aspect of the present invention, the fossil shellfish has a particle size of 5
The stress response inhibitor is a powder containing at least 50% by weight, preferably at least 80% by weight, having a particle size of 0 μm or less.

When the above-mentioned shell fossil is the above-mentioned powder, it becomes a cloudy state, easily disperses in water, and becomes a stress reaction inhibitor which is easy to handle when added to and mixed with a transport container containing a marine animal. In general, the smaller the particle size of shell fossils, the better the adsorption performance. However, if the particle size is smaller than the small holes for adsorbing harmful substances, it is meaningless and the cost of grinding increases. On the other hand, if the particle size is too large, the particles immediately settle even if they are added to and mixed with water in the transport container, and the white turbidity effect cannot be obtained. Therefore, the particle size is in the range of 2 μm to 100 μm, and with the above particle size distribution, the white turbidity effect and the adsorption effect can be simultaneously satisfied in a well-balanced manner, and the performance can be maintained.

According to a fourth aspect of the present invention, there is provided a method for transporting live fish in which a marine animal is transported alive with water in a transport container on a vehicle, wherein 1 ton of water is contained in the transport container. The stress response inhibitor according to 1, 2, or 3,
g to 800 g, preferably 10 g to 300 g, by adding and mixing, thereby reducing and suppressing stress on the marine animals occurring during the period of transportation of the marine animals by the transport container to maintain health. This is a method for transporting live fish.

Each of the stress response inhibitors containing these three kinds of shell fossils as main components respectively has a length of transport time, a water temperature,
Depending on factors such as the type of marine animals and the accommodation density, the ratio of addition to 1 ton of water varies, but if it is less than 4 g, it is not possible to alleviate and suppress stress on marine animals and maintain health, and if it is more than 800 g, conversely Depending on the type of marine animal, it may cause stress, and in addition, the cost may be increased, which may have undesirable results. In this specification, the definition of transport includes everything from picking up marine animals from farms and offshore to passing them on to the final user, and therefore includes both livestock and live fish transport,
In the case of raising livestock, the stress response inhibitor is most preferably in a range of 10 to 100 g per 1 ton of water.
A range of 00 g is most desirable. In the case of live fish transportation,
300 g or more, for example, 500 g of the stress response inhibitor
It is also possible to add more than a certain amount of marine animals in the shipping container by adding in excess. The method of adding and mixing the stress reaction inhibitor with water is not particularly limited, and any method may be used. In the case of transporting live fish, it is important to keep the water constantly stirred and opaque, and if the aeration equipment is installed in the vehicle, use it. That is, shell fossils were directly added to the aeration position of the aeration equipment, or shells were packed in a net, shell fossils were packed in a non-woven fabric, and shell fossils were encapsulated in a non-woven fabric in a cell shape. It is better to put a thing or a thing impregnated with shell fossils, and keep the cloudy state while mixing the shell fossils.

The invention according to claim 5 is a method for transporting live fish in which the stress response inhibitor is dissolved in water in another container when the stress response inhibitor is added to and mixed with the transport container. In this method, water is placed in another container, a stress reaction inhibitor is added thereto, and the mixture is stirred and mixed to make it turbid, and then the turbid liquid is placed in a shipping container. At that time, if the turbid liquid is immediately diffused into a turbid state if it is placed in the aeration position of the aeration equipment of the transport container as described above, the turbidity effect and the adsorption effect are simultaneously satisfied with a good balance, and the performance is maintained. It will be easier.

According to a sixth aspect of the present invention, there is provided a method for transporting live fish in which water is constantly stirred during transportation of the marine animal by the transport container. If the water is constantly stirred, the shell fossils do not settle, the water becomes cloudy, and the shell fossils can be kept dispersed, so the chance of contact with harmful substances and carbon dioxide in the water increases, It becomes easier to adsorb and absorb, which is convenient for water quality conservation.

According to a seventh aspect of the present invention, there is provided a live fish transported by the method for transporting a live fish according to the fourth, fifth or sixth aspect. The live fish transported by the above-mentioned method for transporting live fish are not simply live fish but lively and healthy fish due to various effects of the stress response inhibitor.

[0027]

[Example 1]

1. Testing period December 26-27, 1997 (Heisei 9) 2. Test fish Adult red sea bream 3. Red sea bream capacity 200 liters of seawater in a 200 liter panlight tank
Put 10 red sea bream (11 to 12 kg) per aquarium. 4. Test water temperature Natural seawater temperature 18 ° C and heated seawater temperature 25 °
Perform with C. Note that the temperature was raised to 25 ° C. by placing natural seawater at a water temperature of 18 ° C. in a water tank and accommodating test fish.
Do it over time. 5. Test time 8 hours and 24 hours 6. 6. Amount of added stress response inhibitor 30 g of stress response inhibitor containing shell fossil as a main component per 100 l of seawater (300 g of stress response inhibitor per ton of seawater) Oxygen supply Air and pure oxygen are supplied to maintain saturation. 8. Test items Water quality measurement Water temperature, salt concentration, pH, ammonia nitrogen, COD (Chemical Oxygen Demand) Blood property test Cortisol, hemoglobin, hematocrit Observation Observation of body color and photography For comparison, the above test items The test is also performed on the control group to which the stress response inhibitor is not added. The temperature of natural seawater was 18.0 ° C, and the salt concentration at this time was 3
4.65 °. Table 3 shows the pH, Table 4 shows the ammonia nitrogen, Table 5 shows the COD,
Table 4 shows the results of blood property tests of cortisol amount, hemoclobin amount and hematocrit value, respectively.

[0028]

[Table 3]

According to Table 3, the pH of natural seawater is 8.0, and the pH of seawater in the water tank after 8 hours is 0.4 to 0.5.
Although there was a decrease, there was no difference between the group in which the stress response inhibitor was added (hereinafter simply referred to as “addition group”) and the control group irrespective of the water temperature. However, while the pH after 24 hours is constant without change from the pH after 8 hours regardless of the water temperature,
The pH of the control decreased by 0.3 to 0.4. This indicates that the stress reaction inhibitor of the present invention suppresses a decrease in pH and absorbs carbon dioxide in seawater in the aquarium. Therefore, the carbon dioxide in the seawater in the aquarium gradually decreases, so that the fish can efficiently take oxygen.

[0030]

[Table 4]

According to Table 4, the values of ammonia nitrogen after 8 hours and 24 hours of seawater in the water tank were lower in the addition group than in the control group, regardless of the water temperature. What should be noted is 1
After 8 hours, the ammonia nitrogen in the addition zone at 8 ° C was 0.7
Although it is 7 mg at / l, 0.68 mg after 24 hours
at / l. In the still-water tank, although the amount of ammonia nitrogen derived from fish excreta increases with time, the decrease clearly indicates that the stress response inhibitor of the present invention is adsorbed accordingly. .

[0032]

[Table 5]

According to Table 5, C after 8 hours of seawater in the water tank
As for OD, the addition group showed a considerably lower value than the control group at both 18 ° C. and 25 ° C. However, after 24 hours COD
Showed no difference between the addition group and the control group regardless of the water temperature.

[0034]

[Table 6]

According to Table 6, the amount of cortisol, which is the best indicator reflecting the stress response of fish, is below the measurement limit of 1.0 μg / dl under all conditions in the added group, and is considerably higher than that in the control group. Low, indicating that the stress response inhibitor of the present invention works well. Regarding the hemoglobin amount and the hematocrit value, the measured values are different in the addition group and the control group, and no difference can be recognized. This is a measured value in a short period of at most 24 hours, and it is estimated that the individual difference may have appeared in the measured value as it is.

In the above-mentioned observation, the results of the visual observation of the body color of each of the three addition groups and the control group after the elapse of 24 hours show that the three individual addition groups have a pale red color. On the other hand, each of the three fishes in the control group was dark and had poor color development.

Next, a test is performed on red sea bream. [Example 2] 1. Test period January 6-7, 1998 (Heisei 10) 2. Test fish Red sea bream fry 3. Red Sea Bream Capacity 200 liters of seawater are placed in a 200 liter panlight aquarium, and 200 red sea bream juveniles (average length 8 cm) are placed in each aquarium. 4. Test water temperature Natural seawater temperature 17 ° C and heated seawater temperature 24 °
Perform with C. In addition, after raising the temperature to 24 ° C, natural water of 17 ° C of the water temperature was put in the water tank, and the test fish was stored.
Perform over 5 hours. 5. Test time 4 hours, 8 hours, 24 hours 6. Addition amount of stress response inhibitor 15 g of stress response inhibitor mainly composed of shell fossils per 50 l of seawater (300 g of stress response inhibitor per ton of seawater) Oxygen supply Air is supplied to maintain almost saturation. 8. Test items Water quality measurement Water temperature, salt concentration, pH, dissolved oxygen, ammonia nitrogen, COD (Chemical Oxygen Demand) For comparison, for the above test items, a test was also performed for a control plot without the addition of a stress response inhibitor. I do. The temperature of natural seawater was 17.6 ° C, and the salt concentration at this time was 3 ° C.
It was 4.72 °. Table 5 shows the pH and dissolved oxygen.
Table 6 shows ammonia nitrogen, and Table 7 shows COD.

[0038]

[Table 7]

According to Table 7, the pH of natural seawater was 8.0, and the pH of the seawater in the water tank after 24 hours was 7.8 irrespective of the water temperature, while the control group was pH 7.8 regardless of the water temperature. Water temperature is 17
PH 7.3 at 24 ° C., pH 7.4 at 24 ° C.,
It is lower by 0.3 to 0.4 than in the case of the addition group. Therefore, in the addition group, the carbon dioxide in the seawater in the aquarium is clearly reduced as compared with the control group, so that the fish can efficiently take oxygen. In addition, pH of dissolved oxygen
This indicates that the control group consumes more oxygen than the added group and makes it more difficult for the fish to ingest oxygen.

[0040]

[Table 8]

According to Table 8, the ammonia nitrogen at 8 hours and 24 hours after the seawater in the water tank had a lower value in the addition section than in the control section regardless of the water temperature. Therefore, the advantage of the stress response inhibitor of the present invention is recognized.

[0042]

[Table 9]

According to Table 9, after 4 hours of seawater in the tank, 8
The COD after the lapse of time showed the same or higher values in the addition group than in the control group at both the water temperatures of 17 ° C. and 24 ° C. However, the COD after 24 hours showed a significantly lower value in the addition group than in the control group regardless of the water temperature.

Next, an observation test of the stress state in the aquarium is performed on the adult red sea bream. [Example 3] 1. Test period June 6-7, 1998 (Heisei 10) 2. Test fish Adult red sea bream 3. Red sea bream capacity 40 liters of seawater are placed in a 100 liter acrylic water tank, and three red sea bream fish (average body weight 1.2 kg) are put in each water tank. 4. Test water temperature 25 ° C 5. Testing time 24 hours 6. 6. Addition amount of stress response inhibitor 15 g of stress response inhibitor mainly composed of shell fossils per 40 l of seawater (375 g of stress response inhibitor per 1 ton of seawater) Oxygen supply Air is supplied to maintain almost saturation. 8. Test items Observation (1) Visual observation and photography of the test fish in the aquarium. (2) Visual observation and photographing of the test fish immediately after killing immediately after 24 hours. (3) Immediately after 24 hours, immerse the test fish in ice water for about 10 minutes, and visually observe and photograph the fish. For comparison, the above test items are also tested in a control group to which no stress response inhibitor is added.

In the above observation (1), it was found that the red sea bream in the control group had extremely poor darkening color, whereas the red sea bream in the added group maintained the color peculiar to the red sea bream for 24 hours. Also, in the observation of (2), the red sea bream in the control group was close to the original body color, but also had a darkened body color, whereas the red sea bream in the added group had a beautiful body color. further,
In the observation in (3), when viewed from both the ventral and dorsal sides of the fish, the red sea bream in the control group was slightly pale and slightly darkened, whereas the red sea bream in the added group had a good body color for the most part. Presented.

Next, the surviving rate of adult red sea bream is determined under severe conditions, and the advantage of the stress response inhibitor of the present invention is confirmed. [Embodiment 4] 1. Test period June 16-17, 1998 (Heisei 10) 2. Test fish Adult red sea bream 3. Red sea bream capacity 40 liters of seawater are placed in a 100 liter acrylic water tank, and eight red sea bream fish per tank (total weight about 9 kg, about 22.5% based on water volume). 4. Test water temperature 25 ° C to 26 ° C 5. Testing time 24 hours 6. 6. Addition amount of stress response inhibitor 50 g of stress response inhibitor containing shell fossil as a main component per 100 l of seawater (500 g of stress response inhibitor per ton of seawater) Oxygen supply Air is supplied to maintain almost saturation. 8. Test items Observation (1) Survival rate after 5 hours (survival number) (2) Survival rate after 10 hours (survival number) (3) Survival rate after 24 hours (survival number) For comparison, the above test items , The same test is performed for the control group to which the stress response inhibitor is not added.

The conventional general storage capacity varies somewhat depending on the type of marine animal, but is about 10% by weight of the water volume. By the way, red sea bream is 10%. In this test, the storage capacity was 22.5%, which was twice or more. Table 8 shows the results.

[0048]

[Table 10]

According to Table 10, the survival rates after 5 hours were all alive in the added group and died in the control group. Also,
The survival rate after 10 hours was all alive in the addition group, and two of them survived in the control group since four died. And 24
In the survival rate after 4 hours, 4 animals died and 4 animals survived in the addition group. In the control group, the survival was zero because one animal died. Therefore, it can be said that the live fish can be transported within a transport time of about 5 hours if the stress response inhibitor of the present invention is added in a somewhat large amount at about twice the general capacity.

[0050]

As described in detail above, the stress response inhibitor of the present invention, the method for transporting live fish using the same, and the live fish thereof have the following effects. According to the invention of claim 1, since it is only necessary to add and mix a stress response inhibitor containing shell fossils as a main component to a transport container in which a marine animal is housed, it is necessary to install a large-scale facility or to install an existing facility. With almost no modification required, for various stressors such as high-density storage, light, water quality deterioration due to excrement,
It can reduce and eliminate the stress response of marine animals, and can transport and deliver truly vibrant and healthy marine animals intact and delivered to end users.

According to the second aspect of the present invention, in addition to the above-mentioned effects, the water quality preservation effect other than the cloudiness effect becomes clearer, and a truly vibrant and healthy marine animal is transported while maintaining the same state. And make it easier to deliver to the end user.

According to the third aspect of the present invention, in addition to the above effects, it can be easily dispersed and immediately exerted a function as an inhibitor of a stress reaction.

According to the fourth aspect of the present invention, the use of the stress response inhibitor containing shell fossils as a main component enables the marine plant to be transported in a truly vibrant and healthy state.

The invention of claim 5 provides a method for adding, mixing, and adding a stress response inhibitor to a transport container accommodating a marine animal.
Easy to disperse.

According to the sixth aspect of the present invention, it is easy to maintain the white turbidity effect and the water quality preservation effect of the stress reaction inhibitor.

The invention of claim 7 can be obtained even when a truly lively and healthy marine animal is away from the landing site.

Claims (7)

[Claims] (1) Various types of nekton, plankton, algae, seaweed, and the like made of calcareous or silicic acid are buried and deposited, and the main component is shell fossil, which is a humus-soluble crystalline body, and keeps aquatic animals alive. A stress response suppressant characterized by alleviating and suppressing a stress response occurring in a marine animal when transported. 2. The fossil shellfish is formed by burying and depositing various nekton, plankton, algae, seaweed, and the like made of calcareous or silicic acid, etc., and forming a humus-soluble crystal at 150 ° C. to 300 ° C.
2. The stress reaction inhibitor according to claim 1, wherein the agent is activated by removing the water of crystallization by heat treatment within the range of C. 3. The stress response inhibitor according to claim 1, wherein the shell fossil is a powder containing at least 50% by weight, preferably at least 80% by weight, of particles having a particle size of 50 μm or less. 4. A method for transporting live fish, wherein a live animal is transported alive together with water in a transport container on the vehicle.
The method according to claim 1, wherein the transport container comprises a step of adding and mixing the stress reaction inhibitor according to claim 1, 2 or 3 at a ratio of 4 g to 800 g, preferably 10 g to 300 g per 1 ton of water. A method for transporting live fish, characterized by reducing or suppressing stress on marine animals during transportation of the marine animals by the transport container to maintain health. 5. The method for transporting live fish according to claim 4, wherein said stress reaction inhibitor is dissolved in water in another container when added to and mixed with said transport container. 6. The method for transporting live fish according to claim 4, wherein the water is constantly stirred during the transportation of the marine animal by the transport container. 7. A live fish transported by the method for transporting live fish according to claim 4, 5 or 6.