ITRM20040438A1 - Metodo per la purificazione e l'amplificazione di cellule staminali tumorali. - Google Patents

Metodo per la purificazione e l'amplificazione di cellule staminali tumorali.

Info

Publication number
ITRM20040438A1
ITRM20040438A1 IT000438A ITRM20040438A ITRM20040438A1 IT RM20040438 A1 ITRM20040438 A1 IT RM20040438A1 IT 000438 A IT000438 A IT 000438A IT RM20040438 A ITRM20040438 A IT RM20040438A IT RM20040438 A1 ITRM20040438 A1 IT RM20040438A1
Authority
IT
Italy
Prior art keywords
amplification
purification
stem cells
cancer stem
cancer
Prior art date
Application number
IT000438A
Other languages
English (en)
Inventor
Giorgio Stassi
Matilde Todaro
Original Assignee
Univ Palermo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Palermo filed Critical Univ Palermo
Priority to IT000438A priority Critical patent/ITRM20040438A1/it
Publication of ITRM20040438A1 publication Critical patent/ITRM20040438A1/it
Priority to US11/662,487 priority patent/US20070292389A1/en
Priority to PCT/IT2005/000523 priority patent/WO2006030473A1/en
Priority to EP08012238A priority patent/EP2006374A3/en
Priority to CA002580645A priority patent/CA2580645A1/en
Priority to EP05794564A priority patent/EP1805299A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C12N5/0695—Stem cells; Progenitor cells; Precursor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C12N2501/392—Sexual steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin
IT000438A 2004-09-15 2004-09-15 Metodo per la purificazione e l'amplificazione di cellule staminali tumorali. ITRM20040438A1 (it)

Priority Applications (6)

Application Number Priority Date Filing Date Title
IT000438A ITRM20040438A1 (it) 2004-09-15 2004-09-15 Metodo per la purificazione e l'amplificazione di cellule staminali tumorali.
US11/662,487 US20070292389A1 (en) 2004-09-15 2005-09-14 Method for the Purification and Amplification of Tumoral Stem Cells
PCT/IT2005/000523 WO2006030473A1 (en) 2004-09-15 2005-09-14 Method for the purification and amplification of tumoral stem cells
EP08012238A EP2006374A3 (en) 2004-09-15 2005-09-14 Method for the purification and amplification of tumoral stem cells
CA002580645A CA2580645A1 (en) 2004-09-15 2005-09-14 Method for the purification and amplification of tumoral stem cells
EP05794564A EP1805299A1 (en) 2004-09-15 2005-09-14 Method for the purification and amplification of tumoral stem cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT000438A ITRM20040438A1 (it) 2004-09-15 2004-09-15 Metodo per la purificazione e l'amplificazione di cellule staminali tumorali.

Publications (1)

Publication Number Publication Date
ITRM20040438A1 true ITRM20040438A1 (it) 2004-12-15

Family

ID=35511004

Family Applications (1)

Application Number Title Priority Date Filing Date
IT000438A ITRM20040438A1 (it) 2004-09-15 2004-09-15 Metodo per la purificazione e l'amplificazione di cellule staminali tumorali.

Country Status (5)

Country Link
US (1) US20070292389A1 (it)
EP (2) EP1805299A1 (it)
CA (1) CA2580645A1 (it)
IT (1) ITRM20040438A1 (it)
WO (1) WO2006030473A1 (it)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2629330C (en) 2004-11-12 2018-05-22 Cambridge University Technical Services Ltd. Methods and means related to cancer stem cells
EP2049147A2 (en) * 2006-07-06 2009-04-22 Apogenix GmbH Human il-4 muteins in combination with chemotherapeutics or pro-apoptotic agents in cancer therapy
GB2442059A (en) 2006-09-19 2008-03-26 Ist Superiore Sanita Test for cancer of the gastrointestinal tract
WO2008091908A2 (en) 2007-01-22 2008-07-31 Raven Biotechnologies Human cancer stem cells
EP2318040A4 (en) * 2008-07-24 2013-05-01 Univ Central Florida Res Found THERAPY TREATED ON CANCER STAMPS
EP2529238A4 (en) 2010-01-28 2014-10-08 The Regents Of The University Of Michigan HANGING DRIPPING DEVICES, SYSTEMS AND / OR METHODS
CN102424817B (zh) * 2011-12-31 2013-11-06 广州呼吸疾病研究所 一种来源于人肺腺癌的细胞株及其制备方法
DE102012003700B3 (de) * 2012-02-28 2012-11-29 FLACOD GmbH Verfahren zur Trennung von Verbänden maligner Zellen und Verbänden aus Stromazellen einer Malignomgewebeprobe
EP2951291A1 (en) * 2013-02-01 2015-12-09 Euroclone Spa Serum-free culture medium for cancer stem cells
DE102013010528B3 (de) * 2013-06-25 2014-02-20 FLACOD GmbH Verfahren zur Erstellung einer Datenbank für die Vorabschätzung der Wirksamkeit von Wirkstoffen in der Tumortherapie
JP2018521962A (ja) 2015-04-30 2018-08-09 ナント ホールディングス アイピー エルエルシーＮａｎｔ Ｈｏｌｄｉｎｇｓ ＩＰ， ＬＬＣ 催奇形性医薬化合物を介した患者処置
WO2017173247A1 (en) * 2016-03-31 2017-10-05 City Of Hope Aptamer compositions and the use thereof
CN105807053B (zh) * 2016-04-15 2019-04-02 苏州药明康德新药开发股份有限公司 一种肿瘤解离试剂在流式细胞检测中的应用
CN108070560A (zh) * 2016-11-15 2018-05-25 江苏齐氏生物科技有限公司 一种人原代胃癌细胞的分离及培养方法
CN108070559A (zh) * 2016-11-15 2018-05-25 江苏齐氏生物科技有限公司 一种原代人乳腺癌细胞分离及培养方法
GB201700952D0 (en) * 2017-01-19 2017-03-08 Univ Cape Town In vitro propagation of primary cancer cells
US10767164B2 (en) 2017-03-30 2020-09-08 The Research Foundation For The State University Of New York Microenvironments for self-assembly of islet organoids from stem cells differentiation
US20200377862A1 (en) * 2017-04-21 2020-12-03 Younsei University Industry Foundation Method for preparing cancer stemness cell line through metabolic stress, and cancer cell prepared through same
AU2018347796A1 (en) 2017-10-10 2020-05-07 Medicenna Therapeutics, Inc. IL-4-fusion formulations for treatment of central nervous system (CNS) tumors
EP3896154A4 (en) * 2018-06-13 2022-03-09 Genex Health Co.,Ltd PRIMARY COLORECTAL CANCER SOLID TUMOR CELL AND METHOD FOR CULTIVATING PRIMARY COLORECTAL CANCER ASCITIC FLUID TUMOR CELLS, AND MATCHING REAGENT
CN112760287A (zh) * 2019-11-04 2021-05-07 北京基石生命科技有限公司 一种用于培养泌尿肿瘤实体瘤原代细胞的培养基
CN113817665A (zh) * 2021-09-13 2021-12-21 哈尔滨中科赛恩斯生物技术有限公司 一种小鼠乳腺癌细胞的分离培养及传代培养方法
CN114480290A (zh) * 2021-12-15 2022-05-13 武汉巴菲尔生物技术服务有限公司 一种诱导肿瘤细胞形成肿瘤干细胞的方法
CN115369071A (zh) * 2022-05-26 2022-11-22 上海墨卓生物科技有限公司 一种用于不同组织解离的普适性方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6329148B1 (en) * 1999-02-16 2001-12-11 The Board Of Trustees Of The Leland Stanford University Combined therapy of diterpenoid triepoxides and death domain receptor ligands for synergistic killing of tumor cells
US6984522B2 (en) * 2000-08-03 2006-01-10 Regents Of The University Of Michigan Isolation and use of solid tumor stem cells
EP1639090A4 (en) * 2003-06-09 2008-04-16 Univ Michigan COMPOSITIONS AND METHODS FOR TREATING AND DIAGNOSING CANCER
CA2447400A1 (en) * 2003-09-12 2005-03-12 The Hospital For Sick Children Brain tumor stem cells

Also Published As

Publication number Publication date
EP1805299A1 (en) 2007-07-11
US20070292389A1 (en) 2007-12-20
EP2006374A3 (en) 2009-05-27
EP2006374A2 (en) 2008-12-24
WO2006030473A1 (en) 2006-03-23
CA2580645A1 (en) 2006-03-23

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