IT1271433B - Procedimento per trattare un'immagine di una frazione nell'elettroforesi. - Google Patents
Procedimento per trattare un'immagine di una frazione nell'elettroforesi.Info
- Publication number
- IT1271433B IT1271433B ITMI930448A ITMI930448A IT1271433B IT 1271433 B IT1271433 B IT 1271433B IT MI930448 A ITMI930448 A IT MI930448A IT MI930448 A ITMI930448 A IT MI930448A IT 1271433 B IT1271433 B IT 1271433B
- Authority
- IT
- Italy
- Prior art keywords
- electrophoresis
- positions
- model
- lengths
- image
- Prior art date
Links
- 238000001962 electrophoresis Methods 0.000 title abstract 5
- 238000000034 method Methods 0.000 title 1
- 238000005259 measurement Methods 0.000 abstract 3
- 238000005194 fractionation Methods 0.000 abstract 2
- 210000002966 serum Anatomy 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Un modello elettroforetico ottenuto sottoponendo a elettroforesi siero umano normale viene normalizzato cosicché siano rilevati punti di riferimento (pa, pb) associati con almeno due predeterminate lunghezze di sviluppo di elettroforesi in un'immagine elettroforetica e questi punti di riferimento sono fissati per livellarsi con predeterminate posizioni di dati (100, 200) associate alle precitate lunghezze. Viene similmente normalizzato un modello di misurazione ottenuto sottoponendo un campione ad elettroforesi. Si rileva un punto minimo del modello di misurazione fra posizioni di picco del modello di riferimento, e si determinano le localizzazioni di posizioni di punto di frazionamento (f1, f2, f3, f4) fra frazioni (a', b', c', d', e') sul modello di misurazione, sulla base dei risultati del rilevamento. E' così possibile determinare in breve cori precisione le posizioni del punto di frazionamento sul modello senza influenza di variazioni in lunghezze di sviluppo o posizione di elettroforesi fra i campioni.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP04055359A JP3130629B2 (ja) | 1992-03-13 | 1992-03-13 | 電気泳動における分画処理方法 |
Publications (3)
Publication Number | Publication Date |
---|---|
ITMI930448A0 ITMI930448A0 (it) | 1993-03-09 |
ITMI930448A1 ITMI930448A1 (it) | 1994-09-09 |
IT1271433B true IT1271433B (it) | 1997-05-28 |
Family
ID=12996302
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ITMI930448A IT1271433B (it) | 1992-03-13 | 1993-03-09 | Procedimento per trattare un'immagine di una frazione nell'elettroforesi. |
Country Status (3)
Country | Link |
---|---|
JP (1) | JP3130629B2 (it) |
DE (1) | DE4307736C2 (it) |
IT (1) | IT1271433B (it) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7537938B2 (en) | 2000-04-28 | 2009-05-26 | Monogram Biosciences, Inc. | Biomarker detection in circulating cells |
AU2002310012A1 (en) | 2001-05-21 | 2002-12-03 | Monogram Biosciences, Inc. | Methods and compositions for analyzing proteins |
EP1490533A4 (en) * | 2002-04-02 | 2007-07-04 | Aclara Biosciences Inc | MULTIPLEXASSAYS USING ELECTROPHORETICALLY SEPARATED MOLECULAR MARKERS |
JP4756707B2 (ja) * | 2007-02-22 | 2011-08-24 | アングルトライ株式会社 | スペクトル波形パターンのピーク位置検出方法又は領域分割方法およびプログラム |
JP6740941B2 (ja) * | 2016-08-05 | 2020-08-19 | 株式会社島津製作所 | 電気泳動測定方法、データ処理装置及びデータ処理プログラム |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS55156851A (en) * | 1979-05-25 | 1980-12-06 | Olympus Optical Co Ltd | Fractionation processing method for electrophoresis |
JPS5670455A (en) * | 1979-11-13 | 1981-06-12 | Olympus Optical Co Ltd | Processing method for demarcation in electrophoresis |
JPS5719650A (en) * | 1980-07-10 | 1982-02-01 | Olympus Optical Co Ltd | Split processing method in electrophoresis |
JPS5719666A (en) * | 1980-07-10 | 1982-02-01 | Olympus Optical Co Ltd | Fractionation treating method in electrophoresis |
US4920498A (en) * | 1985-08-17 | 1990-04-24 | Olympus Optical Co., Ltd. | Method of processing and analyzing electrophoretic image, and method of displaying electrophoregram and a medium for recording electrophoregram |
-
1992
- 1992-03-13 JP JP04055359A patent/JP3130629B2/ja not_active Expired - Fee Related
-
1993
- 1993-03-09 IT ITMI930448A patent/IT1271433B/it active IP Right Grant
- 1993-03-11 DE DE4307736A patent/DE4307736C2/de not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
JPH05256819A (ja) | 1993-10-08 |
DE4307736A1 (en) | 1993-09-23 |
ITMI930448A0 (it) | 1993-03-09 |
DE4307736C2 (de) | 1998-06-10 |
ITMI930448A1 (it) | 1994-09-09 |
JP3130629B2 (ja) | 2001-01-31 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
0001 | Granted | ||
TA | Fee payment date (situation as of event date), data collected since 19931001 |
Effective date: 19970328 |