IN2014DN11191A - - Google Patents

Info

Publication number
IN2014DN11191A
IN2014DN11191A IN11191DEN2014A IN2014DN11191A IN 2014DN11191 A IN2014DN11191 A IN 2014DN11191A IN 11191DEN2014 A IN11191DEN2014 A IN 11191DEN2014A IN 2014DN11191 A IN2014DN11191 A IN 2014DN11191A
Authority
IN
India
Prior art keywords
support substrate
magnetic particles
magnetic
magnetic field
aggregations
Prior art date
Application number
Inventor
Koshin Hamasaki
Toshiro Saito
Original Assignee
Hitachi High Tech Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi High Tech Corp filed Critical Hitachi High Tech Corp
Publication of IN2014DN11191A publication Critical patent/IN2014DN11191A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6489—Photoluminescence of semiconductors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

Abstract

In order to quantify a trace amount of a biomolecule biomolecules are specifically captured with magnetic particles and the biomolecules are labeled with fluorescence. The present invention addresses the problems of an efficient fixed reaction of the magnetic particles to a support substrate and of breaking up aggregations of the magnetic particles. A magnetic field generator for attracting the magnetic particles to the support substrate that can be switched on or off is provided on the reverse face of the support substrate and an adhesion layer is provided on the surface of the support substrate to hold the magnetic particles. First a dispersing solution for the magnetic particles is placed on the surface of the support substrate with the magnetic field for the support substrate surface in an off state. Next the magnetic field is turned on and the magnetic particles in solution are attracted to the support substrate surface. The magnetic particles colliding with the support substrate adhere to the adhesion layer of the support substrate surface and then the magnetic field is turned off. Thus aggregations can be broken up while the magnetic particles are held and a magnetic particle layer on the support substrate can be a single layer.
IN11191DEN2014 2012-07-06 2013-06-12 IN2014DN11191A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2012151993 2012-07-06
PCT/JP2013/066143 WO2014007034A1 (en) 2012-07-06 2013-06-12 Analysis device and analysis method

Publications (1)

Publication Number Publication Date
IN2014DN11191A true IN2014DN11191A (en) 2015-10-02

Family

ID=49881794

Family Applications (1)

Application Number Title Priority Date Filing Date
IN11191DEN2014 IN2014DN11191A (en) 2012-07-06 2013-06-12

Country Status (6)

Country Link
US (1) US9964539B2 (en)
EP (1) EP2871464B1 (en)
JP (1) JP5998217B2 (en)
CN (1) CN104471380B (en)
IN (1) IN2014DN11191A (en)
WO (1) WO2014007034A1 (en)

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WO2016151143A1 (en) * 2015-03-26 2016-09-29 Koninklijke Philips N.V. Manufacturing of a biosensor cartridge
PL413909A1 (en) 2015-09-15 2017-03-27 Wrocławskie Centrum Badań Eit + Spółka Z Ograniczoną Odpowiedzialnością Method for detection and selection of hybridoma cells producing the desirable antibodies
JP6783154B2 (en) * 2016-01-31 2020-11-11 アークレイ株式会社 Analysis equipment
JP7130919B2 (en) 2016-03-11 2022-09-06 三菱瓦斯化学株式会社 Biological material immobilization method
KR102026521B1 (en) * 2016-05-26 2019-09-30 서울대학교산학협력단 Kit and Method for Detecting Target Materials using Asymmetric Beads
WO2018208840A1 (en) 2017-05-08 2018-11-15 Dweik Majed Apparatus and method for detecting microbial contamination
CN107290534A (en) * 2017-07-12 2017-10-24 华讯方舟科技有限公司 A kind of blood cell capture chip and method
US10585028B2 (en) 2017-10-20 2020-03-10 Charted Scientific, Inc. Method and apparatus for optical analysis
US11041756B2 (en) 2017-10-20 2021-06-22 Charted Scientific Inc. Method and apparatus of filtering light using a spectrometer enhanced with additional spectral filters with optical analysis of fluorescence and scattered light from particles suspended in a liquid medium using confocal and non confocal illumination and imaging
JP7369017B2 (en) 2018-11-26 2023-10-25 デンカ株式会社 Analyte detection sensitization method using colloidal particles
CN113227342A (en) * 2019-01-09 2021-08-06 株式会社日立高新技术 Substrate for nucleic acid analysis, flow cell for nucleic acid analysis, and image analysis method
US20220276165A1 (en) * 2019-07-11 2022-09-01 Citizen Watch Co., Ltd. Device for detecting substance being measured
CN113376146A (en) * 2020-02-25 2021-09-10 上海交通大学 Detection particle suitable for biomolecule multiplex detection and preparation method and application thereof
WO2023178604A1 (en) * 2022-03-24 2023-09-28 京东方科技集团股份有限公司 Quantum dot light-emitting device and quantum dot layer patterning method

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Also Published As

Publication number Publication date
JPWO2014007034A1 (en) 2016-06-02
US20150153335A1 (en) 2015-06-04
US9964539B2 (en) 2018-05-08
EP2871464A1 (en) 2015-05-13
CN104471380B (en) 2017-11-17
WO2014007034A1 (en) 2014-01-09
CN104471380A (en) 2015-03-25
EP2871464B1 (en) 2021-07-28
EP2871464A4 (en) 2016-01-27
JP5998217B2 (en) 2016-09-28

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