IN2014CN03937A - - Google Patents

Info

Publication number
IN2014CN03937A
IN2014CN03937A IN3937CHN2014A IN2014CN03937A IN 2014CN03937 A IN2014CN03937 A IN 2014CN03937A IN 3937CHN2014 A IN3937CHN2014 A IN 3937CHN2014A IN 2014CN03937 A IN2014CN03937 A IN 2014CN03937A
Authority
IN
India
Prior art keywords
amino acid
seq
acid sequence
proteases
show
Prior art date
Application number
Inventor
Hendrik Hellmuth
Marion Merkel
Brian Laufs
Susanne Wieland
Timothy OCONNELL
Susanne Tondera
Thomas Weber
Original Assignee
Henkel Ag & Co Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Henkel Ag & Co Kgaa filed Critical Henkel Ag & Co Kgaa
Publication of IN2014CN03937A publication Critical patent/IN2014CN03937A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Proteases comprising an amino acid sequence that is at least 70% identical to the amino acid sequence given in SEQ ID NO: 1 over its total length and that have R99E or R99D amino acid substitution in the numbering according to SEQ ID NO: 1 in combination with at least two additional amino acid substitutions selected from the group consisting of S3T V4I and V199I show a very good purification performance especially for blood containing fouling and excellent temperature stability.
IN3937CHN2014 2011-10-28 2012-10-19 IN2014CN03937A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102011118021A DE102011118021A1 (en) 2011-10-28 2011-10-28 Performance-enhanced and temperature-stable protease variants
PCT/EP2012/070721 WO2013060621A1 (en) 2011-10-28 2012-10-19 Performance-enhanced and temperature-resistant protease variants

Publications (1)

Publication Number Publication Date
IN2014CN03937A true IN2014CN03937A (en) 2015-09-04

Family

ID=47022726

Family Applications (1)

Application Number Title Priority Date Filing Date
IN3937CHN2014 IN2014CN03937A (en) 2011-10-28 2012-10-19

Country Status (11)

Country Link
US (3) US20140227764A1 (en)
EP (1) EP2771459B1 (en)
JP (1) JP6498440B2 (en)
KR (1) KR102026497B1 (en)
CN (2) CN108384772A (en)
DE (1) DE102011118021A1 (en)
DK (1) DK2771459T3 (en)
ES (1) ES2752449T3 (en)
IN (1) IN2014CN03937A (en)
PL (1) PL2771459T3 (en)
WO (1) WO2013060621A1 (en)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102012215107A1 (en) 2012-08-24 2014-02-27 Basf Se Solid dishwashing detergent with improved protease performance
DE102013218253A1 (en) 2013-09-12 2015-03-12 Henkel Ag & Co. Kgaa Solid laundry detergent with improved protease performance
EP3399031B1 (en) 2014-12-15 2019-10-30 Henkel AG & Co. KGaA Detergent composition comprising subtilase variants
DE102014226681A1 (en) 2014-12-19 2016-06-23 Henkel Ag & Co. Kgaa Liquid surfactant composition with special surfactant combination and enzyme
DE102015208655A1 (en) * 2015-05-11 2016-11-17 Henkel Ag & Co. Kgaa enzyme stabilizers
CA3005292A1 (en) 2015-12-09 2017-06-15 Basf Se Method of purifying a protein from fermentation solids under desorbing conditions
BR112018077483A2 (en) 2016-07-14 2019-04-02 Basf Se fermentation medium, methods for cultivating a microorganism and for producing a compound of interest, trace element solution for a fermentation process, and use of a chelating agent.
DE102016221849A1 (en) 2016-11-08 2018-05-09 Henkel Ag & Co. Kgaa A surfactant composition containing an amylase
US10047321B2 (en) 2016-12-22 2018-08-14 Henkel Ag & Co. Kgaa Liquid surfactant compositions having a modified oxo-alcohol derivative
US10385291B2 (en) 2016-12-22 2019-08-20 Henkel Ag & Co. Kgaa Liquid surfactant compositions and associated methods
BR112020009093A2 (en) 2017-11-09 2020-10-13 Basf Se enzyme particle, washing or cleaning and food or feed compositions, and use of an organic white pigment
EP3660151B1 (en) 2018-11-29 2023-07-19 Henkel AG & Co. KGaA Variant proteases having improved performance and storage stability
EP3660146B1 (en) 2018-11-29 2023-11-15 Henkel AG & Co. KGaA Storage stable protease variants with improved performance
DE102019204792A1 (en) 2019-04-04 2020-10-08 Henkel Ag & Co. Kgaa Use of mannanase enzyme in combination with catechol derivatives
EP3770237A1 (en) 2019-07-22 2021-01-27 Henkel AG & Co. KGaA Washing and cleaning agents with improved enzyme stability
EP3770241A1 (en) 2019-07-22 2021-01-27 Henkel AG & Co. KGaA Cleaning agent with protease for automatic dosing
CN114846128A (en) * 2019-12-20 2022-08-02 汉高股份有限及两合公司 Cleaning compositions comprising disperse protein VIII
DE102020205400A1 (en) 2020-04-29 2021-11-04 Henkel Ag & Co. Kgaa Highly alkaline laundry detergent with protease
DE102020205381A1 (en) 2020-04-29 2021-11-04 Henkel Ag & Co. Kgaa Highly alkaline laundry detergent with protease
DE102022205588A1 (en) 2022-06-01 2023-12-07 Henkel Ag & Co. Kgaa DETERGENT AND CLEANING AGENTS WITH IMPROVED ENZYME STABILITY
DE102022205594A1 (en) 2022-06-01 2023-12-07 Henkel Ag & Co. Kgaa PERFORMANCE-IMPROVED AND STORAGE-STABLE PROTEASE VARIANTS
DE102022208891A1 (en) 2022-08-16 2024-02-22 Henkel Ag & Co. Kgaa PERFORMANCE IMPROVED PROTEASE VARIANTS
WO2024037686A1 (en) 2022-08-16 2024-02-22 Henkel Ag & Co. Kgaa Performance-enhanced protease variants x
WO2024037685A1 (en) 2022-08-16 2024-02-22 Henkel Ag & Co. Kgaa Performance-enhanced protease variants ix
DE102022208890A1 (en) 2022-08-16 2024-02-22 Henkel Ag & Co. Kgaa PERFORMANCE IMPROVED PROTEASE VARIANTS IX
EP4324900A1 (en) * 2022-08-17 2024-02-21 Henkel AG & Co. KGaA Detergent composition comprising enzymes
DE102022209245A1 (en) 2022-09-06 2024-03-07 Henkel Ag & Co. Kgaa DETERGENT AND CLEANING PRODUCT CONTAINING TANNASE I
DE102022209246A1 (en) 2022-09-06 2024-03-07 Henkel Ag & Co. Kgaa DETERGENT AND CLEANING PRODUCT CONTAINING TANNASE II

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5340735A (en) 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
JP3922391B2 (en) * 1994-02-24 2007-05-30 ヘンケル コマンディットゲゼルシャフト アウフ アクティーン Improved enzyme and detergent containing the same
ES2364774T3 (en) * 1994-02-24 2011-09-14 HENKEL AG & CO. KGAA IMPROVED AND DETERGENT ENZYMES THAT CONTAIN THEM.
DE19700327A1 (en) * 1997-01-08 1998-07-09 Henkel Kgaa Protease and amylase detergent
DE19824705A1 (en) * 1998-06-03 1999-12-09 Henkel Kgaa Detergents and cleaning agents containing amylase and protease
BR0215383A (en) 2001-12-31 2006-11-28 Genencor Int proteases that produce altered immunogenic response and methods of manufacture and use thereof
DE102004019751A1 (en) 2004-04-23 2005-11-17 Henkel Kgaa Novel Alkaline Proteases and Detergents Containing These Novel Alkaline Proteases
DE102007041754A1 (en) * 2007-09-04 2009-03-05 Henkel Ag & Co. Kgaa Polycyclic compounds as enzyme stabilizers
DE102008017103A1 (en) 2008-04-02 2009-10-08 Henkel Ag & Co. Kgaa Detergents and cleaning agents containing proteases from Xanthomonas
DE102009029513A1 (en) 2009-09-16 2011-03-24 Henkel Ag & Co. Kgaa Storage-stable liquid washing or cleaning agent containing proteases
CN102652175B (en) 2009-12-09 2016-02-10 宝洁公司 Fabric and household care product

Also Published As

Publication number Publication date
KR20140085570A (en) 2014-07-07
CN103906836A (en) 2014-07-02
US20210207063A1 (en) 2021-07-08
JP2015502141A (en) 2015-01-22
EP2771459B1 (en) 2019-09-04
WO2013060621A1 (en) 2013-05-02
KR102026497B1 (en) 2019-09-27
DE102011118021A1 (en) 2013-05-02
US20140227764A1 (en) 2014-08-14
ES2752449T3 (en) 2020-04-06
US20170037343A1 (en) 2017-02-09
CN108384772A (en) 2018-08-10
EP2771459A1 (en) 2014-09-03
DK2771459T3 (en) 2019-12-09
PL2771459T3 (en) 2020-03-31
JP6498440B2 (en) 2019-04-10
US10975335B2 (en) 2021-04-13

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