IL84702A - Expression vectors for expression of a protein in higher eukaryotic host cells comprising an enhancer/promoter unit, a protein encoding sequence, signal sequences, and selection marker gene, host cells transformed thereby, a method for producing the protein using the transformed host cells and host cells transformed by the vectors for cloning and multiplication of the vectors - Google Patents

Expression vectors for expression of a protein in higher eukaryotic host cells comprising an enhancer/promoter unit, a protein encoding sequence, signal sequences, and selection marker gene, host cells transformed thereby, a method for producing the protein using the transformed host cells and host cells transformed by the vectors for cloning and multiplication of the vectors

Info

Publication number
IL84702A
IL84702A IL8470287A IL8470287A IL84702A IL 84702 A IL84702 A IL 84702A IL 8470287 A IL8470287 A IL 8470287A IL 8470287 A IL8470287 A IL 8470287A IL 84702 A IL84702 A IL 84702A
Authority
IL
Israel
Prior art keywords
host cells
vectors
protein
transformed
expression
Prior art date
Application number
IL8470287A
Other languages
English (en)
Original Assignee
Ciba Geigy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ciba Geigy filed Critical Ciba Geigy
Publication of IL84702A publication Critical patent/IL84702A/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
IL8470287A 1984-06-14 1987-12-03 Expression vectors for expression of a protein in higher eukaryotic host cells comprising an enhancer/promoter unit, a protein encoding sequence, signal sequences, and selection marker gene, host cells transformed thereby, a method for producing the protein using the transformed host cells and host cells transformed by the vectors for cloning and multiplication of the vectors IL84702A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB8415186A GB8415186D0 (en) 1984-06-14 1984-06-14 Polypeptides

Publications (1)

Publication Number Publication Date
IL84702A true IL84702A (en) 1993-07-08

Family

ID=10562425

Family Applications (1)

Application Number Title Priority Date Filing Date
IL8470287A IL84702A (en) 1984-06-14 1987-12-03 Expression vectors for expression of a protein in higher eukaryotic host cells comprising an enhancer/promoter unit, a protein encoding sequence, signal sequences, and selection marker gene, host cells transformed thereby, a method for producing the protein using the transformed host cells and host cells transformed by the vectors for cloning and multiplication of the vectors

Country Status (6)

Country Link
EP (1) EP0182838A1 (fr)
JP (1) JPS61502377A (fr)
DK (1) DK70486D0 (fr)
GB (1) GB8415186D0 (fr)
IL (1) IL84702A (fr)
WO (1) WO1986000089A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8607502D0 (en) * 1986-03-26 1986-04-30 Antibioticos Sa Penicillium chrysogenum
US4861614A (en) * 1988-02-19 1989-08-29 General Mills, Inc. Instant traditional oatmeal and method of preparation
WO1989009219A1 (fr) * 1988-03-24 1989-10-05 The General Hospital Corporation Vecteur de chromosome artificiel
US5270201A (en) * 1988-03-24 1993-12-14 The General Hospital Corporation Artificial chromosome vector
US6004776A (en) * 1989-07-07 1999-12-21 Unilever Patent Holdings, B.V. Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector
DE69033633T2 (de) * 1989-07-07 2001-05-03 Unilever Nv Verfahren zur Herstellung eines Proteins mittels eines durch Mehrfachkopie-Integrierung eines Expressionsvektors tranformierten Pilzes
US5674728A (en) * 1993-11-03 1997-10-07 Novartis Corporation Aspergillus niger vacuolar aspartyl protease
CA2265519A1 (fr) * 1996-09-24 1998-04-02 Plant Genetic Systems N.V. Produits de synthese d'adn et procedes pour produire des proteines a l'aide de ces produits de synthese d'adn
US6475725B1 (en) 1997-06-20 2002-11-05 Baxter Aktiengesellschaft Recombinant cell clones having increased stability and methods of making and using the same
US9757330B2 (en) 2013-10-18 2017-09-12 Industrial Technology Research Institute Recipe for in-situ gel, and implant, drug delivery system formed thereby

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2125047B (en) * 1982-08-09 1986-02-19 Ciba Geigy Ag Yeast hybrid vectors and their use for the production of polypeptides
AU572108B2 (en) * 1983-01-19 1988-05-05 Genentech Inc. Human tpa production using vectors coding for dhfr protein

Also Published As

Publication number Publication date
DK70486A (da) 1986-02-13
GB8415186D0 (en) 1984-07-18
JPS61502377A (ja) 1986-10-23
DK70486D0 (da) 1986-02-13
WO1986000089A1 (fr) 1986-01-03
EP0182838A1 (fr) 1986-06-04

Similar Documents

Publication Publication Date Title
IL62553A0 (en) Vectors comprising dna sequences and methods for producing recombinant dna molecules and cloning vectors
AU2535784A (en) Recombinant plasmid containing prochymosin coding gene
EP0722461A4 (fr) Expression de polypeptides de fusion transportes hors du cytoplasme sans sequence de tete
GB8424454D0 (en) Recombinant dna cloning vectors
EP0182372A3 (fr) Polypeptides facteur VIII coagulant
IL84702A (en) Expression vectors for expression of a protein in higher eukaryotic host cells comprising an enhancer/promoter unit, a protein encoding sequence, signal sequences, and selection marker gene, host cells transformed thereby, a method for producing the protein using the transformed host cells and host cells transformed by the vectors for cloning and multiplication of the vectors
AU615413B2 (en) Production of thrombin inhibitors
AU3802595A (en) Tripeptidyl aminopeptidase
AU577810B2 (en) Cloning the gene for rabbit tumor necrosis factor polypeptide
AU7489098A (en) Polypeptides having aminopeptidase activity and nucleic acids encoding same
GR3033769T3 (en) Human interferon beta2A, vectors containing genes coding for said interferon, cell lines producing same.
ES8306177A1 (es) Un metodo de proporcionar una muestra de un gen fusionado que codifica un polipeptido eucariotico o procariotico deseado.
EP0132309A3 (fr) Elément promoteur eucaryotique réglable
DK0453969T3 (fr)
ES8706825A1 (es) Un procedimiento para preparar un plasmido recombinante
AU3950285A (en) Recombinant DNA expression vectors and method for gene expression
AU3870285A (en) Cloning vectors containing selectable genetic markers
EP0165759A3 (fr) Promoteurs mammifères utiles pour l'expression dans des levures
ES8704204A1 (es) Un procedimiento para preparar el plasmido pks3
SG74694G (en) Recombinant immunoglobulin preparations, methods for their preparation, DNA sequences, expression vectors and recombinant host cells therefor
IE840578L (en) Cloning factor viii gene
IL172076A0 (en) Nucleic acids, vectors and host cells encoding and expressing heregulin 2-??, method of making polypeptides using the same, heregulin 2-?? polypeptides and compositions containing them
GB8714414D0 (en) Cloning genes
IE840059L (en) Recombinant dna expression vectors