IL43378A - Aqueous buffer solution for use as a plasma substitute - Google Patents
Aqueous buffer solution for use as a plasma substituteInfo
- Publication number
- IL43378A IL43378A IL43378A IL4337873A IL43378A IL 43378 A IL43378 A IL 43378A IL 43378 A IL43378 A IL 43378A IL 4337873 A IL4337873 A IL 4337873A IL 43378 A IL43378 A IL 43378A
- Authority
- IL
- Israel
- Prior art keywords
- buffer solution
- aqueous buffer
- alkali metal
- salt
- carcinoembryonic antigen
- Prior art date
Links
- 239000012062 aqueous buffer Substances 0.000 title claims 13
- 239000000243 solution Substances 0.000 title claims 13
- 239000003058 plasma substitute Substances 0.000 title claims 2
- 108010022366 Carcinoembryonic Antigen Proteins 0.000 claims abstract 6
- 239000000427 antigen Substances 0.000 claims abstract 4
- 210000002381 plasma Anatomy 0.000 claims abstract 4
- 102000036639 antigens Human genes 0.000 claims abstract 3
- 108091007433 antigens Proteins 0.000 claims abstract 3
- 230000005764 inhibitory process Effects 0.000 claims abstract 3
- 238000000954 titration curve Methods 0.000 claims abstract 3
- 102100025475 Carcinoembryonic antigen-related cell adhesion molecule 5 Human genes 0.000 claims 6
- 229910052783 alkali metal Inorganic materials 0.000 claims 5
- 150000001340 alkali metals Chemical class 0.000 claims 5
- 150000003839 salts Chemical class 0.000 claims 5
- FXKZPKBFTQUJBA-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;sodium;dihydrate Chemical compound O.O.[Na].[Na].OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O FXKZPKBFTQUJBA-UHFFFAOYSA-N 0.000 claims 4
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical group [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims 4
- 239000003755 preservative agent Substances 0.000 claims 3
- 230000002335 preservative effect Effects 0.000 claims 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims 2
- 150000008044 alkali metal hydroxides Chemical class 0.000 claims 2
- 229940098773 bovine serum albumin Drugs 0.000 claims 2
- 239000012141 concentrate Substances 0.000 claims 2
- 239000000463 material Substances 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 244000005700 microbiome Species 0.000 claims 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 1
- 101000914324 Homo sapiens Carcinoembryonic antigen-related cell adhesion molecule 5 Proteins 0.000 claims 1
- 101000914321 Homo sapiens Carcinoembryonic antigen-related cell adhesion molecule 7 Proteins 0.000 claims 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- 239000007853 buffer solution Substances 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 239000008367 deionised water Substances 0.000 claims 1
- 239000012153 distilled water Substances 0.000 claims 1
- 229960001484 edetic acid Drugs 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 150000007524 organic acids Chemical class 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 238000004448 titration Methods 0.000 claims 1
- 102000012406 Carcinoembryonic Antigen Human genes 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/96—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood or serum control standard
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/967—Standards, controls, materials, e.g. validation studies, buffer systems
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/811—Test for named disease, body condition or organ function
- Y10S436/813—Cancer
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/106664—Blood serum or blood plasma standard or control
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/108331—Preservative, buffer, anticoagulant or diluent
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicinal Preparation (AREA)
- Peptides Or Proteins (AREA)
Abstract
A composition suitable for use as a substitute for blood plasma extract in producing antiserum titration curves and standard antigen inhibition curves for the determination of carcinoembryonic antigen in human plasma is disclosed.
[US3852415A]
Claims (16)
1. An aqueous buffer solution suitable for use as a plasma substitute during the production of antiserum titration ' .curves-and-s-tanda-rd-an-tigen—i-nhibi-tion-curves~for-th~e~~d¾"ter-mination of carcinoembryonic antigen in human plasma having a pH of about 6.5 comprising per liter, a. salt of ethylenediamine tetraacetic acid with an alkali metal in an amount equivalent to about 1.3 grams per liter of disodium ethylenediamine tetraacetic acid dihydrate, a preservative to inhibit the growth of microorganisms, and an alkali metal hydroxide.
2. An aqueous buffer solution as claimed in claim 1 wherein the alkali metal of the salt is the same as the alkali metal of the hydroxide.
3. An aqueous buffer solution as claimed in claim 1 or claim 2 wherein the alkali metal is sodium.
4. An aqueous buffer solution as. claimed in any one of the preceding claims wherein the salt is disodium ethylenediamine tetraacetic acid dihydrate.
5. An aqueous- buffer- solution- as~ claimed i an one of the preceding claims wherein the salt is present in an amount equivalent to 1.25 to 1.32 gm/liter of disodium ethylenediamine tetraacetic acid dihydrate. 43378/2
6. An aqueous buffer solution as claimed in anyone of the preceding claims having a pH of from 6.45 to 6.55.
7. An aqueous buffer solution as claimed in any one of the preceding claims also containing a proteinaceous material which is inert to CEA antibody-antigen reactions.
8. An aqueous buffer solution as claimed in claim 7, containing, as the proteinaceous material, bovine serum albumin.
9. An aqueous buffer solution as claimed in claim 8 containing 70 ϋΐβΓβ/ϋΐβΓ of bovine serum albumin.
10. An aqueous buffer solution as claimed in any one of the preceding claims wherein the preservative is sodium azide.
11. .11. An aqueous buffer solution as claimed in claim 10 containing 0.17 gm/liter of sodium azide. 43378/2
12. A concentrate suitable for the preparation, by the addition of deionized or distilled water, of an aqueous buffer solution as claimed in any one of the preceding claims.
13. A concentrate as claimed in claim 12 having an pH of about 6.25 comprising, a salt of an organic acid with an alkali metal in an amount equivalent to about 13 grams per liter of disodium ethylenediamine tetra- acetic acid dihydrate, a preservative to inhibit, the growth of microorganisms* and an alkali metal hydroxide.
14. An aqueous buffer solution in concentrated or diluted form as claimed in any one of the preceding claims substantially as hereinbefore described with particular reference to Examples 1 and 2.
15. A process for the preparation of a carcinoembryonic antigen antiserum titration curve or a carcinoembryonic antigen standard antigen inhibition curve, wherein in place of blood plasma extract a buffer solution as claimed in any one of claims 1 to 11 is employed.
16. A process for the preparation of a carcinoembryonic antigen antiserum titration curve or an carcinoembryonic antigen standard antigen inhibition curve substantially as hereinbefore described with particular reference to the foregoing Examples 5/ 6, 8 and 9· AN/PAJHNj
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US00297565A US3852415A (en) | 1972-10-13 | 1972-10-13 | Compositions for use in radioimmunoassay as a substitute for blood plasma extract in determination of carcinoembryonic antigen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL43378A0 IL43378A0 (en) | 1974-01-14 |
| IL43378A true IL43378A (en) | 1976-04-30 |
Family
ID=23146844
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL43378A IL43378A (en) | 1972-10-13 | 1973-10-04 | Aqueous buffer solution for use as a plasma substitute |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US3852415A (en) |
| JP (1) | JPS5315130B2 (en) |
| AU (1) | AU467511B2 (en) |
| BE (1) | BE805991A (en) |
| CA (1) | CA1012458A (en) |
| CH (1) | CH584409A5 (en) |
| DD (1) | DD108151A5 (en) |
| DE (1) | DE2349738C3 (en) |
| FR (1) | FR2203525A5 (en) |
| GB (1) | GB1423244A (en) |
| IL (1) | IL43378A (en) |
| IT (1) | IT1001584B (en) |
| NL (1) | NL161884C (en) |
| SE (1) | SE417460B (en) |
| ZA (2) | ZA723264B (en) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3901870A (en) * | 1974-03-12 | 1975-08-26 | Behringwerke Ag | Derivative of alpha' 1'-fetospecific serum protein and process for its manufacture |
| US4132769A (en) * | 1974-10-30 | 1979-01-02 | Osther Kurt B | Cancer antigen, cancer therapy, and cancer diagnosis |
| US3999944A (en) * | 1975-02-28 | 1976-12-28 | Hoffmann-La Roche Inc. | Detection of breast cancer |
| US4043757A (en) * | 1975-05-20 | 1977-08-23 | Ortho Pharmaceutical Corporation | Method for detection of human mammary carcinoma |
| US4152410A (en) * | 1975-09-03 | 1979-05-01 | Eisai Co., Ltd. | Diagnosis reagent for neoplasm and method for diagnosis of neoplasm |
| US4379839A (en) * | 1977-05-23 | 1983-04-12 | The Trustees Of Columbia University In The City Of New York | Method for detecting cancer |
| JPS562557A (en) * | 1979-06-22 | 1981-01-12 | Green Cross Corp:The | Aqueous solvent for coagulation test |
| US4349528A (en) * | 1979-11-21 | 1982-09-14 | The Wistar Institute | Monocolonal hybridoma antibody specific for high molecular weight carcinoembryonic antigen |
| JPS57208459A (en) * | 1981-06-19 | 1982-12-21 | Eisai Co Ltd | Measuring method using enzyme-labelled antibody and reagent |
| US4631254A (en) * | 1982-12-06 | 1986-12-23 | Hoffmann-La Roche Inc. | Carcinoembryonic antigen determination |
| US4624916A (en) * | 1984-04-06 | 1986-11-25 | International Immunoassay Laboratories, Inc. | Process and composition for the rapid quantitation of small levels of creative kinase-MB isoenzyme |
| JPS61280566A (en) * | 1984-09-03 | 1986-12-11 | Hiroaki Sawai | Direct immunoassay of 2'-5'-oligoadenylic acid |
| US4690772A (en) * | 1985-06-03 | 1987-09-01 | National Medical Care | Sterilant compositions |
| US6013772A (en) * | 1986-08-13 | 2000-01-11 | Bayer Corporation | Antibody preparations specifically binding to unique determinants of CEA antigens or fragments thereof and use of the antibody preparations in immunoassays |
| CA1296253C (en) * | 1986-10-20 | 1992-02-25 | Praveen Tyle | Stabilized growth hormone compositions |
| US5296377A (en) * | 1992-12-15 | 1994-03-22 | Boehringer Mannheim Corporation | Control reagent containing a hydroxylamine or an antioxidant |
| AU2011254206B2 (en) | 2010-05-20 | 2015-06-25 | Ecolab Usa Inc. | Rheology modified low foaming liquid antimicrobial compositions and methods of use thereof |
| KR102384708B1 (en) * | 2020-06-19 | 2022-04-11 | 대한민국 | Artificial blood for bloodstain pattern analysis |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3720760A (en) * | 1968-09-06 | 1973-03-13 | Pharmacia Ab | Method for determining the presence of reagin-immunoglobulins(reagin-ig)directed against certain allergens,in aqueous samples |
| US3697638A (en) * | 1970-06-01 | 1972-10-10 | Hoffmann La Roche | Antigens |
-
1972
- 1972-05-15 ZA ZA723264A patent/ZA723264B/en unknown
- 1972-10-13 US US00297565A patent/US3852415A/en not_active Expired - Lifetime
-
1973
- 1973-10-01 CH CH1403573A patent/CH584409A5/xx not_active IP Right Cessation
- 1973-10-01 ZA ZA737693A patent/ZA737693B/en unknown
- 1973-10-02 CA CA182,470A patent/CA1012458A/en not_active Expired
- 1973-10-03 DE DE2349738A patent/DE2349738C3/en not_active Expired
- 1973-10-04 IL IL43378A patent/IL43378A/en unknown
- 1973-10-08 IT IT29875/73A patent/IT1001584B/en active
- 1973-10-10 AU AU61242/73A patent/AU467511B2/en not_active Expired
- 1973-10-11 FR FR7336328A patent/FR2203525A5/fr not_active Expired
- 1973-10-12 SE SE7313919A patent/SE417460B/en unknown
- 1973-10-12 JP JP11463773A patent/JPS5315130B2/ja not_active Expired
- 1973-10-12 GB GB4780473A patent/GB1423244A/en not_active Expired
- 1973-10-12 DD DD174034A patent/DD108151A5/xx unknown
- 1973-10-12 BE BE136609A patent/BE805991A/en not_active IP Right Cessation
- 1973-10-12 NL NL7314093.A patent/NL161884C/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| DE2349738C3 (en) | 1981-06-19 |
| DE2349738B2 (en) | 1978-01-19 |
| NL161884B (en) | 1979-10-15 |
| JPS5315130B2 (en) | 1978-05-23 |
| US3852415A (en) | 1974-12-03 |
| NL7314093A (en) | 1974-04-16 |
| AU6124273A (en) | 1975-05-01 |
| FR2203525A5 (en) | 1974-05-10 |
| JPS4971133A (en) | 1974-07-10 |
| SE417460B (en) | 1981-03-16 |
| CA1012458A (en) | 1977-06-21 |
| BE805991A (en) | 1974-04-12 |
| AU467511B2 (en) | 1975-12-04 |
| ZA737693B (en) | 1974-08-28 |
| IT1001584B (en) | 1976-04-30 |
| DD108151A5 (en) | 1974-09-05 |
| CH584409A5 (en) | 1977-01-31 |
| IL43378A0 (en) | 1974-01-14 |
| DE2349738A1 (en) | 1974-04-18 |
| ZA723264B (en) | 1974-08-28 |
| GB1423244A (en) | 1976-02-04 |
| NL161884C (en) | 1980-03-17 |
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