IL34930A - Haemolytic assay system - Google Patents
Haemolytic assay systemInfo
- Publication number
- IL34930A IL34930A IL34930A IL3493070A IL34930A IL 34930 A IL34930 A IL 34930A IL 34930 A IL34930 A IL 34930A IL 3493070 A IL3493070 A IL 3493070A IL 34930 A IL34930 A IL 34930A
- Authority
- IL
- Israel
- Prior art keywords
- complement
- agar
- gel
- hemolysin
- antigen
- Prior art date
Links
- 230000002949 hemolytic effect Effects 0.000 title description 6
- 238000003556 assay Methods 0.000 title description 2
- 230000000295 complement effect Effects 0.000 claims description 66
- 239000000427 antigen Substances 0.000 claims description 40
- 108091007433 antigens Proteins 0.000 claims description 40
- 102000036639 antigens Human genes 0.000 claims description 40
- 238000000034 method Methods 0.000 claims description 29
- 239000000499 gel Substances 0.000 claims description 28
- 210000003743 erythrocyte Anatomy 0.000 claims description 26
- 229920001817 Agar Polymers 0.000 claims description 22
- 210000002966 serum Anatomy 0.000 claims description 21
- 238000006243 chemical reaction Methods 0.000 claims description 14
- 108010006464 Hemolysin Proteins Proteins 0.000 claims description 12
- 239000003228 hemolysin Substances 0.000 claims description 12
- 239000013060 biological fluid Substances 0.000 claims description 11
- 238000009792 diffusion process Methods 0.000 claims description 11
- 239000000203 mixture Substances 0.000 claims description 11
- 239000008272 agar Substances 0.000 claims description 7
- 239000007787 solid Substances 0.000 claims description 5
- 241001494479 Pecora Species 0.000 description 16
- 238000012360 testing method Methods 0.000 description 15
- 210000004027 cell Anatomy 0.000 description 12
- 230000009089 cytolysis Effects 0.000 description 10
- 239000003349 gelling agent Substances 0.000 description 9
- 230000000951 immunodiffusion Effects 0.000 description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 7
- 235000010419 agar Nutrition 0.000 description 6
- 238000010790 dilution Methods 0.000 description 5
- 239000012895 dilution Substances 0.000 description 5
- 241000700199 Cavia porcellus Species 0.000 description 4
- -1 polyethylene Polymers 0.000 description 4
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000223205 Coccidioides immitis Species 0.000 description 2
- 206010014979 Epidemic typhus Diseases 0.000 description 2
- 206010018910 Haemolysis Diseases 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000711386 Mumps virus Species 0.000 description 2
- 241000606651 Rickettsiales Species 0.000 description 2
- 206010039207 Rocky Mountain Spotted Fever Diseases 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- KKEYFWRCBNTPAC-UHFFFAOYSA-N Terephthalic acid Chemical compound OC(=O)C1=CC=C(C(O)=O)C=C1 KKEYFWRCBNTPAC-UHFFFAOYSA-N 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 2
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- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
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- ZZSNKZQZMQGXPY-UHFFFAOYSA-N Ethyl cellulose Chemical compound CCOCC1OC(OC)C(OCC)C(OCC)C1OC1C(O)C(O)C(OC)C(CO)O1 ZZSNKZQZMQGXPY-UHFFFAOYSA-N 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 241000206672 Gelidium Species 0.000 description 1
- GVGLGOZIDCSQPN-PVHGPHFFSA-N Heroin Chemical compound O([C@H]1[C@H](C=C[C@H]23)OC(C)=O)C4=C5[C@@]12CCN(C)[C@@H]3CC5=CC=C4OC(C)=O GVGLGOZIDCSQPN-PVHGPHFFSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
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- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- NNLVGZFZQQXQNW-ADJNRHBOSA-N [(2r,3r,4s,5r,6s)-4,5-diacetyloxy-3-[(2s,3r,4s,5r,6r)-3,4,5-triacetyloxy-6-(acetyloxymethyl)oxan-2-yl]oxy-6-[(2r,3r,4s,5r,6s)-4,5,6-triacetyloxy-2-(acetyloxymethyl)oxan-3-yl]oxyoxan-2-yl]methyl acetate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](OC(C)=O)[C@H]1OC(C)=O)O[C@H]1[C@@H]([C@@H](OC(C)=O)[C@H](OC(C)=O)[C@@H](COC(C)=O)O1)OC(C)=O)COC(=O)C)[C@@H]1[C@@H](COC(C)=O)O[C@@H](OC(C)=O)[C@H](OC(C)=O)[C@H]1OC(C)=O NNLVGZFZQQXQNW-ADJNRHBOSA-N 0.000 description 1
- 150000001252 acrylic acid derivatives Chemical class 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
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- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
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- 235000010418 carrageenan Nutrition 0.000 description 1
- 229920001525 carrageenan Polymers 0.000 description 1
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- 229960002069 diamorphine Drugs 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
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- 150000004676 glycans Chemical class 0.000 description 1
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- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 1
- 239000000017 hydrogel Substances 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 239000012678 infectious agent Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
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- 230000000813 microbial effect Effects 0.000 description 1
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- 239000001814 pectin Substances 0.000 description 1
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- 238000009589 serological test Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
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Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US84833869A | 1969-08-07 | 1969-08-07 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL34930A0 IL34930A0 (en) | 1970-09-17 |
| IL34930A true IL34930A (en) | 1973-06-29 |
Family
ID=25303007
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL34930A IL34930A (en) | 1969-08-07 | 1970-07-15 | Haemolytic assay system |
Country Status (4)
| Country | Link |
|---|---|
| JP (1) | JPS5022086B1 (ja) |
| CA (1) | CA937499A (ja) |
| ES (1) | ES382558A1 (ja) |
| IL (1) | IL34930A (ja) |
-
1970
- 1970-07-15 CA CA088272A patent/CA937499A/en not_active Expired
- 1970-07-15 IL IL34930A patent/IL34930A/en unknown
- 1970-08-07 ES ES382558A patent/ES382558A1/es not_active Expired
- 1970-08-07 JP JP6873570A patent/JPS5022086B1/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CA937499A (en) | 1973-11-27 |
| ES382558A1 (es) | 1973-05-01 |
| IL34930A0 (en) | 1970-09-17 |
| JPS5022086B1 (ja) | 1975-07-28 |
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