IL304571A - Methods for the purification of recombinant proteins - Google Patents

Methods for the purification of recombinant proteins

Info

Publication number
IL304571A
IL304571A IL304571A IL30457123A IL304571A IL 304571 A IL304571 A IL 304571A IL 304571 A IL304571 A IL 304571A IL 30457123 A IL30457123 A IL 30457123A IL 304571 A IL304571 A IL 304571A
Authority
IL
Israel
Prior art keywords
purification
methods
recombinant proteins
recombinant
proteins
Prior art date
Application number
IL304571A
Other languages
English (en)
Hebrew (he)
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of IL304571A publication Critical patent/IL304571A/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/10Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1885Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in parallel
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804Affinity chromatography
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/36Extraction; Separation; Purification by a combination of two or more processes of different types
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02Column chromatography
    • G01N30/60Construction of the column
    • G01N30/6034Construction of the column joining multiple columns
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02Column chromatography
    • G01N30/88Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02Column chromatography
    • G01N30/88Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
IL304571A 2021-03-10 2023-07-19 Methods for the purification of recombinant proteins IL304571A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163159217P 2021-03-10 2021-03-10
PCT/US2022/019697 WO2022192504A1 (en) 2021-03-10 2022-03-10 Methods for purification of recombinant proteins

Publications (1)

Publication Number Publication Date
IL304571A true IL304571A (en) 2023-09-01

Family

ID=80952165

Family Applications (1)

Application Number Title Priority Date Filing Date
IL304571A IL304571A (en) 2021-03-10 2023-07-19 Methods for the purification of recombinant proteins

Country Status (9)

Country Link
US (1) US20240051990A1 (https=)
EP (1) EP4305048A1 (https=)
JP (1) JP2024509877A (https=)
KR (1) KR20230154908A (https=)
CN (1) CN117043173A (https=)
AU (1) AU2022234768A1 (https=)
CA (1) CA3209216A1 (https=)
IL (1) IL304571A (https=)
WO (1) WO2022192504A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2024235093A1 (en) * 2023-03-14 2025-09-04 Amgen Inc. Anion exchange chromatography processes using a primary amine ligand
US20240426793A1 (en) * 2023-06-20 2024-12-26 Amgen Inc. Methods for chromatography and chromatography medium reuse
WO2025262319A1 (en) * 2024-06-21 2025-12-26 Boehringer Ingelheim International Gmbh Modifying blue color formation in bioprocesses

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CZ302070B6 (cs) 1998-04-21 2010-09-29 Micromet Ag Jednoretezcový multifunkcní polypeptid, polynukleotid, vektor obsahující tento polynukleotid, bunka transfekovaná tímto polynukleotidem, prostredek obsahující tento polypeptid, polynukleotid nebo vektor a jejich použití a zpusob identifikace aktiváto
DE602004030811D1 (de) 2003-10-16 2011-02-10 Micromet Ag Multispezifische deimmunisierte cd3-bindende moleküle
RS53008B2 (sr) 2007-04-03 2022-12-30 Amgen Res Munich Gmbh Interspecijski specifičan cd3-epsilon vezujući domen
EP2820047B1 (en) 2012-03-01 2018-04-25 Amgen Research (Munich) GmbH Long life polypeptide binding molecules
EP2656892A1 (en) * 2012-04-23 2013-10-30 Merck Patent GmbH Chromatography method
SG10201709131UA (en) * 2013-03-08 2017-12-28 Genzyme Corp Continuous purification of therapeutic proteins
US20140302037A1 (en) 2013-03-15 2014-10-09 Amgen Inc. BISPECIFIC-Fc MOLECULES
AU2014236769B2 (en) 2013-03-15 2018-09-27 Amgen Inc. Heterodimeric bispecific antibodies
US20140308285A1 (en) 2013-03-15 2014-10-16 Amgen Inc. Heterodimeric bispecific antibodies
WO2014140358A1 (en) 2013-03-15 2014-09-18 Amgen Research (Munich) Gmbh Single chain binding molecules comprising n-terminal abp
EP3733710A1 (en) 2013-09-25 2020-11-04 Amgen, Inc Hetrodimeric v-c-fc-v-c antibody
EA039859B1 (ru) 2015-07-31 2022-03-21 Эмджен Рисерч (Мюник) Гмбх Биспецифические конструкты антител, связывающие egfrviii и cd3

Also Published As

Publication number Publication date
US20240051990A1 (en) 2024-02-15
CA3209216A1 (en) 2022-09-15
EP4305048A1 (en) 2024-01-17
AU2022234768A1 (en) 2023-07-27
JP2024509877A (ja) 2024-03-05
KR20230154908A (ko) 2023-11-09
WO2022192504A1 (en) 2022-09-15
CN117043173A (zh) 2023-11-10

Similar Documents

Publication Publication Date Title
IL304571A (en) Methods for the purification of recombinant proteins
IL286745A (en) Continuous production of recombinant proteins
EP3917943A4 (en) PROTEINS PURIFICATION PROCEDURES
EP3521299A4 (en) METHOD FOR PURIFYING RECOMBINANT PROTEINS
IL307832A (en) Engineering of antigen-binding proteins
SG11202012582QA (en) Methods of producing recombinant proteins
EP3247794A4 (en) Method for purification of recombinant human alpha-galactosidase a from material containing contaminant host cell proteins
EP4142905A4 (en) IMPROVED PROCESS FOR PURIFICATION OF PROTEIN
EP4118093A4 (en) METHOD FOR PURIFYING A FC FUSION PROTEIN
IL310595A (en) A standard for determining the glycan profile of proteins
GB201906595D0 (en) Method for purification of plasma proteins
GB202019607D0 (en) Purification of proteins
IL286728A (en) A process for purifying recombinant polypeptides
EP4169938A4 (en) RECOMBINANT PROTEIN PURIFICATION METHOD
PT4263568T (pt) Métodos para a purificação de proteína de fusão fc-péptido reenrolada
GB202018320D0 (en) Methods of producing recombinant complement proteins
HUP2100038A1 (hu) Mutáltatott rekombináns ACE2-Fc fúziós fehérjék COVID-19 fertõzések kezelésére
HK40117981A (en) Method of protein purification
GB202210384D0 (en) Protein purification
GB202210332D0 (en) Protein Purification
GB202110607D0 (en) New uses of proteins
IL308329A (en) A process for the production of recombinant proteins
IL320073A (en) Method for increasing recombinant protein expression
HK40065373A (en) Methods of protein purification
GB202312969D0 (en) Modified proteins