JP2024509877A - 組換えタンパク質の精製方法 - Google Patents

組換えタンパク質の精製方法 Download PDF

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Publication number
JP2024509877A
JP2024509877A JP2023554343A JP2023554343A JP2024509877A JP 2024509877 A JP2024509877 A JP 2024509877A JP 2023554343 A JP2023554343 A JP 2023554343A JP 2023554343 A JP2023554343 A JP 2023554343A JP 2024509877 A JP2024509877 A JP 2024509877A
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Japan
Prior art keywords
chromatography
column
pool
protein
buffer
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JP2023554343A
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English (en)
Japanese (ja)
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JP2024509877A5 (https=
JPWO2022192504A5 (https=
Inventor
ソイス，ニール
コナー，ジェレミー・エス
ハンター，グレン・エム
ショーメイカー，ケネス
ワイリー，ブレット
フリン，ジョン・ビー
ケイオニル，ナコーン
グエン，トゥイ・エヌ
シン，シッダールタ
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アムジエン・インコーポレーテツド
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Publication of JP2024509877A publication Critical patent/JP2024509877A/ja
Publication of JP2024509877A5 publication Critical patent/JP2024509877A5/ja
Publication of JPWO2022192504A5 publication Critical patent/JPWO2022192504A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1885—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in parallel
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6034—Construction of the column joining multiple columns

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  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
JP2023554343A 2021-03-10 2022-03-10 組換えタンパク質の精製方法 Pending JP2024509877A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163159217P 2021-03-10 2021-03-10
US63/159,217 2021-03-10
PCT/US2022/019697 WO2022192504A1 (en) 2021-03-10 2022-03-10 Methods for purification of recombinant proteins

Publications (3)

Publication Number Publication Date
JP2024509877A true JP2024509877A (ja) 2024-03-05
JP2024509877A5 JP2024509877A5 (https=) 2025-03-10
JPWO2022192504A5 JPWO2022192504A5 (https=) 2025-03-10

Family

ID=80952165

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023554343A Pending JP2024509877A (ja) 2021-03-10 2022-03-10 組換えタンパク質の精製方法

Country Status (9)

Country Link
US (1) US20240051990A1 (https=)
EP (1) EP4305048A1 (https=)
JP (1) JP2024509877A (https=)
KR (1) KR20230154908A (https=)
CN (1) CN117043173A (https=)
AU (1) AU2022234768A1 (https=)
CA (1) CA3209216A1 (https=)
IL (1) IL304571A (https=)
WO (1) WO2022192504A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2024235093A1 (en) * 2023-03-14 2025-09-04 Amgen Inc. Anion exchange chromatography processes using a primary amine ligand
US20240426793A1 (en) * 2023-06-20 2024-12-26 Amgen Inc. Methods for chromatography and chromatography medium reuse
WO2025262319A1 (en) * 2024-06-21 2025-12-26 Boehringer Ingelheim International Gmbh Modifying blue color formation in bioprocesses

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015520667A (ja) * 2012-04-23 2015-07-23 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツングＭｅｒｃｋ Ｐａｔｅｎｔ Ｇｅｓｅｌｌｓｃｈａｆｔ ｍｉｔ ｂｅｓｃｈｒａｅｎｋｔｅｒ Ｈａｆｔｕｎｇ クロマトグラフィー法
JP2016510981A (ja) * 2013-03-08 2016-04-14 ジェンザイム・コーポレーション 治療用タンパク質の連続的な精製

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CZ302070B6 (cs) 1998-04-21 2010-09-29 Micromet Ag Jednoretezcový multifunkcní polypeptid, polynukleotid, vektor obsahující tento polynukleotid, bunka transfekovaná tímto polynukleotidem, prostredek obsahující tento polypeptid, polynukleotid nebo vektor a jejich použití a zpusob identifikace aktiváto
DE602004030811D1 (de) 2003-10-16 2011-02-10 Micromet Ag Multispezifische deimmunisierte cd3-bindende moleküle
RS53008B2 (sr) 2007-04-03 2022-12-30 Amgen Res Munich Gmbh Interspecijski specifičan cd3-epsilon vezujući domen
EP2820047B1 (en) 2012-03-01 2018-04-25 Amgen Research (Munich) GmbH Long life polypeptide binding molecules
US20140302037A1 (en) 2013-03-15 2014-10-09 Amgen Inc. BISPECIFIC-Fc MOLECULES
AU2014236769B2 (en) 2013-03-15 2018-09-27 Amgen Inc. Heterodimeric bispecific antibodies
US20140308285A1 (en) 2013-03-15 2014-10-16 Amgen Inc. Heterodimeric bispecific antibodies
WO2014140358A1 (en) 2013-03-15 2014-09-18 Amgen Research (Munich) Gmbh Single chain binding molecules comprising n-terminal abp
EP3733710A1 (en) 2013-09-25 2020-11-04 Amgen, Inc Hetrodimeric v-c-fc-v-c antibody
EA039859B1 (ru) 2015-07-31 2022-03-21 Эмджен Рисерч (Мюник) Гмбх Биспецифические конструкты антител, связывающие egfrviii и cd3

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015520667A (ja) * 2012-04-23 2015-07-23 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツングＭｅｒｃｋ Ｐａｔｅｎｔ Ｇｅｓｅｌｌｓｃｈａｆｔ ｍｉｔ ｂｅｓｃｈｒａｅｎｋｔｅｒ Ｈａｆｔｕｎｇ クロマトグラフィー法
JP2016510981A (ja) * 2013-03-08 2016-04-14 ジェンザイム・コーポレーション 治療用タンパク質の連続的な精製

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JOURNAL OF CHROMATOGRAPHY A, vol. 1579, JPN6026004092, 2018, pages 60 - 72, ISSN: 0005787837 *

Also Published As

Publication number Publication date
US20240051990A1 (en) 2024-02-15
CA3209216A1 (en) 2022-09-15
EP4305048A1 (en) 2024-01-17
AU2022234768A1 (en) 2023-07-27
IL304571A (en) 2023-09-01
KR20230154908A (ko) 2023-11-09
WO2022192504A1 (en) 2022-09-15
CN117043173A (zh) 2023-11-10

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