IL296932B2 - Detection of cytosine modifications at base resolution without bisulfite - Google Patents

Detection of cytosine modifications at base resolution without bisulfite

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Publication number
IL296932B2
IL296932B2 IL296932A IL29693222A IL296932B2 IL 296932 B2 IL296932 B2 IL 296932B2 IL 296932 A IL296932 A IL 296932A IL 29693222 A IL29693222 A IL 29693222A IL 296932 B2 IL296932 B2 IL 296932B2
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IL
Israel
Prior art keywords
dna
nucleic acid
borane
taps
5cac
Prior art date
Application number
IL296932A
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English (en)
Hebrew (he)
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IL296932A (en
IL296932B1 (en
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Ludwig Inst For Cancer Res Ltd
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Publication date
Application filed by Ludwig Inst For Cancer Res Ltd filed Critical Ludwig Inst For Cancer Res Ltd
Publication of IL296932A publication Critical patent/IL296932A/en
Publication of IL296932B1 publication Critical patent/IL296932B1/en
Publication of IL296932B2 publication Critical patent/IL296932B2/en

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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
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    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • C12P17/12—Nitrogen as only ring hetero atom containing a six-membered hetero ring
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    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/16—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing two or more hetero rings
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
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    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6869—Methods for sequencing
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/164—Methylation detection other then bisulfite or methylation sensitive restriction endonucleases
    • C—CHEMISTRY; METALLURGY
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers
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    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01026—DNA alpha-glucosyltransferase (2.4.1.26)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01027—DNA beta-glucosyltransferase (2.4.1.27)

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  • Chemical & Material Sciences (AREA)
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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Biotechnology (AREA)
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  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
IL296932A 2018-01-08 2019-01-08 Detection of cytosine modifications at base resolution without bisulfite IL296932B2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201862614798P 2018-01-08 2018-01-08
US201862660523P 2018-04-20 2018-04-20
US201862771409P 2018-11-26 2018-11-26
PCT/US2019/012627 WO2019136413A1 (en) 2018-01-08 2019-01-08 Bisulfite-free, base-resolution identification of cytosine modifications

Publications (3)

Publication Number Publication Date
IL296932A IL296932A (en) 2022-12-01
IL296932B1 IL296932B1 (en) 2025-09-01
IL296932B2 true IL296932B2 (en) 2026-01-01

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ID=67143801

Family Applications (2)

Application Number Title Priority Date Filing Date
IL296932A IL296932B2 (en) 2018-01-08 2019-01-08 Detection of cytosine modifications at base resolution without bisulfite
IL275850A IL275850B2 (en) 2018-01-08 2019-01-08 Detection of Cytosine Modifications by Bisulfite-Free Base Resolution

Family Applications After (1)

Application Number Title Priority Date Filing Date
IL275850A IL275850B2 (en) 2018-01-08 2019-01-08 Detection of Cytosine Modifications by Bisulfite-Free Base Resolution

Country Status (15)

Country Link
US (5) US12071660B2 (https=)
EP (1) EP3737748A4 (https=)
JP (3) JP7136899B2 (https=)
KR (3) KR20250026369A (https=)
CN (2) CN111971386A (https=)
AU (3) AU2019205416B2 (https=)
BR (1) BR112020013718A2 (https=)
CA (1) CA3087915C (https=)
IL (2) IL296932B2 (https=)
MA (1) MA51633A (https=)
MX (2) MX395068B (https=)
NZ (1) NZ765943A (https=)
SG (1) SG11202006511YA (https=)
WO (1) WO2019136413A1 (https=)
ZA (1) ZA202004769B (https=)

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