IL287541A - Cas9 is engineered with an extended range of target DNA - Google Patents
Cas9 is engineered with an extended range of target DNAInfo
- Publication number
- IL287541A IL287541A IL287541A IL28754121A IL287541A IL 287541 A IL287541 A IL 287541A IL 287541 A IL287541 A IL 287541A IL 28754121 A IL28754121 A IL 28754121A IL 287541 A IL287541 A IL 287541A
- Authority
- IL
- Israel
- Prior art keywords
- broadened
- dna targeting
- engineered cas9
- targeting range
- range
- Prior art date
Links
- 101150038500 cas9 gene Proteins 0.000 title 1
- 230000008685 targeting Effects 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962838498P | 2019-04-25 | 2019-04-25 | |
| PCT/US2020/029855 WO2020219908A1 (en) | 2019-04-25 | 2020-04-24 | Engineered cas9 with broadened dna targeting range |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL287541A true IL287541A (en) | 2021-12-01 |
Family
ID=72941444
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL287541A IL287541A (en) | 2019-04-25 | 2021-10-24 | Cas9 is engineered with an extended range of target DNA |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20220204954A1 (https=) |
| EP (1) | EP3958914A4 (https=) |
| JP (1) | JP2022530055A (https=) |
| KR (1) | KR20220025708A (https=) |
| CN (1) | CN114206394A (https=) |
| AU (1) | AU2020261071A1 (https=) |
| BR (1) | BR112021021306A2 (https=) |
| CA (1) | CA3137903A1 (https=) |
| IL (1) | IL287541A (https=) |
| MX (1) | MX2021012966A (https=) |
| SG (1) | SG11202111814XA (https=) |
| WO (1) | WO2020219908A1 (https=) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2015330699B2 (en) | 2014-10-10 | 2021-12-02 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| EP3823633A4 (en) | 2018-06-29 | 2023-05-03 | Editas Medicine, Inc. | SYNTHETIC LEAD MOLECULES, COMPOSITIONS AND METHODS RELATED THERETO |
| CN115083532B (zh) * | 2022-06-21 | 2024-08-27 | 中国科学技术大学 | 基于分子动力学模拟获取rsv f突变体的方法 |
| CN115927439B (zh) * | 2022-08-29 | 2026-04-21 | 中国农业科学院作物科学研究所 | CRISPR/SaCas9系统在大豆基因编辑中的应用 |
| US20250001010A1 (en) | 2023-06-30 | 2025-01-02 | Christiana Care Gene Editing Institute, Inc. | Nras gene knockout for treatment of cancer |
| CN117866926B (zh) * | 2024-03-07 | 2024-08-16 | 珠海舒桐医疗科技有限公司 | 一种CRISPR-FrCas9蛋白突变体及应用 |
| US20250319206A1 (en) | 2024-04-04 | 2025-10-16 | Christina Care Gene Editing Institute, Inc. | Crispr/cas gene editing of neh4 and/or neh5 domains in nrf2 |
| WO2025226816A1 (en) | 2024-04-23 | 2025-10-30 | Christiana Care Gene Editing Institute, Inc. | Methods of identifying and correlating crispr-induced exon skipping to phenotypic outcomes |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20230156800A (ko) * | 2015-03-03 | 2023-11-14 | 더 제너럴 하스피탈 코포레이션 | 변경된 PAM 특이성을 갖는 조작된 CRISPR-Cas9 뉴클레아제 |
| WO2016205759A1 (en) * | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation |
| US9512446B1 (en) * | 2015-08-28 | 2016-12-06 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| CN110214183A (zh) * | 2016-08-03 | 2019-09-06 | 哈佛大学的校长及成员们 | 腺苷核碱基编辑器及其用途 |
| KR20230125856A (ko) * | 2016-12-23 | 2023-08-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | Pcsk9의 유전자 편집 |
| EP3680331A4 (en) * | 2017-09-05 | 2021-06-09 | The University Of Tokyo | MODIFIED CAS9 PROTEIN AND USE OF IT |
-
2020
- 2020-04-24 EP EP20796242.4A patent/EP3958914A4/en not_active Withdrawn
- 2020-04-24 MX MX2021012966A patent/MX2021012966A/es unknown
- 2020-04-24 US US17/605,932 patent/US20220204954A1/en not_active Abandoned
- 2020-04-24 AU AU2020261071A patent/AU2020261071A1/en not_active Abandoned
- 2020-04-24 CA CA3137903A patent/CA3137903A1/en active Pending
- 2020-04-24 WO PCT/US2020/029855 patent/WO2020219908A1/en not_active Ceased
- 2020-04-24 SG SG11202111814XA patent/SG11202111814XA/en unknown
- 2020-04-24 KR KR1020217038012A patent/KR20220025708A/ko not_active Withdrawn
- 2020-04-24 BR BR112021021306A patent/BR112021021306A2/pt not_active IP Right Cessation
- 2020-04-24 CN CN202080046596.2A patent/CN114206394A/zh active Pending
- 2020-04-24 JP JP2021563030A patent/JP2022530055A/ja active Pending
-
2021
- 2021-10-24 IL IL287541A patent/IL287541A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP3958914A4 (en) | 2023-07-19 |
| JP2022530055A (ja) | 2022-06-27 |
| CN114206394A (zh) | 2022-03-18 |
| KR20220025708A (ko) | 2022-03-03 |
| AU2020261071A1 (en) | 2021-11-25 |
| BR112021021306A2 (pt) | 2022-02-01 |
| MX2021012966A (es) | 2022-04-06 |
| CA3137903A1 (en) | 2020-10-29 |
| SG11202111814XA (en) | 2021-11-29 |
| US20220204954A1 (en) | 2022-06-30 |
| WO2020219908A1 (en) | 2020-10-29 |
| EP3958914A1 (en) | 2022-03-02 |
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