CN114206394A - 一种具有更广dna靶向范围的工程化cas9 - Google Patents

一种具有更广dna靶向范围的工程化cas9 Download PDF

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CN114206394A
CN114206394A CN202080046596.2A CN202080046596A CN114206394A CN 114206394 A CN114206394 A CN 114206394A CN 202080046596 A CN202080046596 A CN 202080046596A CN 114206394 A CN114206394 A CN 114206394A
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sequence
protein
sacas9
amino acid
variant
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丛乐
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Leland Stanford Junior University
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Leland Stanford Junior University
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
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  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202080046596.2A 2019-04-25 2020-04-24 一种具有更广dna靶向范围的工程化cas9 Pending CN114206394A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962838498P 2019-04-25 2019-04-25
US62/838,498 2019-04-25
PCT/US2020/029855 WO2020219908A1 (en) 2019-04-25 2020-04-24 Engineered cas9 with broadened dna targeting range

Publications (1)

Publication Number Publication Date
CN114206394A true CN114206394A (zh) 2022-03-18

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CN202080046596.2A Pending CN114206394A (zh) 2019-04-25 2020-04-24 一种具有更广dna靶向范围的工程化cas9

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US (1) US20220204954A1 (https=)
EP (1) EP3958914A4 (https=)
JP (1) JP2022530055A (https=)
KR (1) KR20220025708A (https=)
CN (1) CN114206394A (https=)
AU (1) AU2020261071A1 (https=)
BR (1) BR112021021306A2 (https=)
CA (1) CA3137903A1 (https=)
IL (1) IL287541A (https=)
MX (1) MX2021012966A (https=)
SG (1) SG11202111814XA (https=)
WO (1) WO2020219908A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115083532A (zh) * 2022-06-21 2022-09-20 中国科学技术大学 基于分子动力学模拟获取rsv f突变体的方法

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2015330699B2 (en) 2014-10-10 2021-12-02 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
EP3823633A4 (en) 2018-06-29 2023-05-03 Editas Medicine, Inc. SYNTHETIC LEAD MOLECULES, COMPOSITIONS AND METHODS RELATED THERETO
CN115927439B (zh) * 2022-08-29 2026-04-21 中国农业科学院作物科学研究所 CRISPR/SaCas9系统在大豆基因编辑中的应用
US20250001010A1 (en) 2023-06-30 2025-01-02 Christiana Care Gene Editing Institute, Inc. Nras gene knockout for treatment of cancer
CN117866926B (zh) * 2024-03-07 2024-08-16 珠海舒桐医疗科技有限公司 一种CRISPR-FrCas9蛋白突变体及应用
US20250319206A1 (en) 2024-04-04 2025-10-16 Christina Care Gene Editing Institute, Inc. Crispr/cas gene editing of neh4 and/or neh5 domains in nrf2
WO2025226816A1 (en) 2024-04-23 2025-10-30 Christiana Care Gene Editing Institute, Inc. Methods of identifying and correlating crispr-induced exon skipping to phenotypic outcomes

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016141224A1 (en) * 2015-03-03 2016-09-09 The General Hospital Corporation Engineered crispr-cas9 nucleases with altered pam specificity
WO2016205759A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation
US20180073012A1 (en) * 2016-08-03 2018-03-15 President And Fellows Of Harvard College Adenosine nucleobase editors and uses thereof
US20180237787A1 (en) * 2016-12-23 2018-08-23 President And Fellows Of Harvard College Gene editing of pcsk9
US20190071657A1 (en) * 2015-08-28 2019-03-07 The General Hospital Corporation Engineered CRISPR-Cas9 Nucleases
WO2019049913A1 (ja) * 2017-09-05 2019-03-14 国立大学法人 東京大学 改変されたＣａｓ９タンパク質及びその用途

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016141224A1 (en) * 2015-03-03 2016-09-09 The General Hospital Corporation Engineered crispr-cas9 nucleases with altered pam specificity
WO2016205759A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation
US20180312824A1 (en) * 2015-06-18 2018-11-01 The Broad Institute Inc. Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation
US20190071657A1 (en) * 2015-08-28 2019-03-07 The General Hospital Corporation Engineered CRISPR-Cas9 Nucleases
US20180073012A1 (en) * 2016-08-03 2018-03-15 President And Fellows Of Harvard College Adenosine nucleobase editors and uses thereof
US20180237787A1 (en) * 2016-12-23 2018-08-23 President And Fellows Of Harvard College Gene editing of pcsk9
WO2019049913A1 (ja) * 2017-09-05 2019-03-14 国立大学法人 東京大学 改変されたＣａｓ９タンパク質及びその用途

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115083532A (zh) * 2022-06-21 2022-09-20 中国科学技术大学 基于分子动力学模拟获取rsv f突变体的方法

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EP3958914A4 (en) 2023-07-19
IL287541A (en) 2021-12-01
JP2022530055A (ja) 2022-06-27
KR20220025708A (ko) 2022-03-03
AU2020261071A1 (en) 2021-11-25
BR112021021306A2 (pt) 2022-02-01
MX2021012966A (es) 2022-04-06
CA3137903A1 (en) 2020-10-29
SG11202111814XA (en) 2021-11-29
US20220204954A1 (en) 2022-06-30
WO2020219908A1 (en) 2020-10-29
EP3958914A1 (en) 2022-03-02

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Application publication date: 20220318