AU2020261071A1 - Engineered Cas9 with broadened DNA targeting range - Google Patents

Engineered Cas9 with broadened DNA targeting range Download PDF

Info

Publication number
AU2020261071A1
AU2020261071A1 AU2020261071A AU2020261071A AU2020261071A1 AU 2020261071 A1 AU2020261071 A1 AU 2020261071A1 AU 2020261071 A AU2020261071 A AU 2020261071A AU 2020261071 A AU2020261071 A AU 2020261071A AU 2020261071 A1 AU2020261071 A1 AU 2020261071A1
Authority
AU
Australia
Prior art keywords
sequence
protein
amino acid
sacas9
pam
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2020261071A
Other languages
English (en)
Inventor
Le Cong
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leland Stanford Junior University
Original Assignee
Leland Stanford Junior University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leland Stanford Junior University filed Critical Leland Stanford Junior University
Publication of AU2020261071A1 publication Critical patent/AU2020261071A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2020261071A 2019-04-25 2020-04-24 Engineered Cas9 with broadened DNA targeting range Abandoned AU2020261071A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962838498P 2019-04-25 2019-04-25
US62/838,498 2019-04-25
PCT/US2020/029855 WO2020219908A1 (en) 2019-04-25 2020-04-24 Engineered cas9 with broadened dna targeting range

Publications (1)

Publication Number Publication Date
AU2020261071A1 true AU2020261071A1 (en) 2021-11-25

Family

ID=72941444

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2020261071A Abandoned AU2020261071A1 (en) 2019-04-25 2020-04-24 Engineered Cas9 with broadened DNA targeting range

Country Status (12)

Country Link
US (1) US20220204954A1 (https=)
EP (1) EP3958914A4 (https=)
JP (1) JP2022530055A (https=)
KR (1) KR20220025708A (https=)
CN (1) CN114206394A (https=)
AU (1) AU2020261071A1 (https=)
BR (1) BR112021021306A2 (https=)
CA (1) CA3137903A1 (https=)
IL (1) IL287541A (https=)
MX (1) MX2021012966A (https=)
SG (1) SG11202111814XA (https=)
WO (1) WO2020219908A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2015330699B2 (en) 2014-10-10 2021-12-02 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
EP3823633A4 (en) 2018-06-29 2023-05-03 Editas Medicine, Inc. SYNTHETIC LEAD MOLECULES, COMPOSITIONS AND METHODS RELATED THERETO
CN115083532B (zh) * 2022-06-21 2024-08-27 中国科学技术大学 基于分子动力学模拟获取rsv f突变体的方法
CN115927439B (zh) * 2022-08-29 2026-04-21 中国农业科学院作物科学研究所 CRISPR/SaCas9系统在大豆基因编辑中的应用
US20250001010A1 (en) 2023-06-30 2025-01-02 Christiana Care Gene Editing Institute, Inc. Nras gene knockout for treatment of cancer
CN117866926B (zh) * 2024-03-07 2024-08-16 珠海舒桐医疗科技有限公司 一种CRISPR-FrCas9蛋白突变体及应用
US20250319206A1 (en) 2024-04-04 2025-10-16 Christina Care Gene Editing Institute, Inc. Crispr/cas gene editing of neh4 and/or neh5 domains in nrf2
WO2025226816A1 (en) 2024-04-23 2025-10-30 Christiana Care Gene Editing Institute, Inc. Methods of identifying and correlating crispr-induced exon skipping to phenotypic outcomes

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20230156800A (ko) * 2015-03-03 2023-11-14 더 제너럴 하스피탈 코포레이션 변경된 PAM 특이성을 갖는 조작된 CRISPR-Cas9 뉴클레아제
WO2016205759A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation
US9512446B1 (en) * 2015-08-28 2016-12-06 The General Hospital Corporation Engineered CRISPR-Cas9 nucleases
CN110214183A (zh) * 2016-08-03 2019-09-06 哈佛大学的校长及成员们 腺苷核碱基编辑器及其用途
KR20230125856A (ko) * 2016-12-23 2023-08-29 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 Pcsk9의 유전자 편집
EP3680331A4 (en) * 2017-09-05 2021-06-09 The University Of Tokyo MODIFIED CAS9 PROTEIN AND USE OF IT

Also Published As

Publication number Publication date
EP3958914A4 (en) 2023-07-19
IL287541A (en) 2021-12-01
JP2022530055A (ja) 2022-06-27
CN114206394A (zh) 2022-03-18
KR20220025708A (ko) 2022-03-03
BR112021021306A2 (pt) 2022-02-01
MX2021012966A (es) 2022-04-06
CA3137903A1 (en) 2020-10-29
SG11202111814XA (en) 2021-11-29
US20220204954A1 (en) 2022-06-30
WO2020219908A1 (en) 2020-10-29
EP3958914A1 (en) 2022-03-02

Similar Documents

Publication Publication Date Title
US20220204954A1 (en) Engineered cas9 with broadened dna targeting range
Tou et al. Precise cut-and-paste DNA insertion using engineered type VK CRISPR-associated transposases
CN105408497B (zh) 使用截短的引导RNA(tru-gRNA)提高RNA引导的基因组编辑的特异性
Findlay et al. Saturation editing of genomic regions by multiplex homology-directed repair
Lister et al. Finding the fifth base: genome-wide sequencing of cytosine methylation
Babu et al. Bridge helix of Cas9 modulates target DNA cleavage and mismatch tolerance
CN112912496A (zh) 提高氨基酸球菌属cpf1的dna切割活性的新型突变
CN111349654B (zh) 使用加标签的向导rna构建体进行高效基因筛选的组合物和方法
EP4165180A2 (en) Engineered mad7 directed endonuclease
WO2024178854A1 (zh) 新型TnpB编程性核酸酶及其应用
WO2021178432A9 (en) Rna-guided genome recombineering at kilobase scale
Köferle et al. CORALINA: a universal method for the generation of gRNA libraries for CRISPR-based screening
Sloan et al. Testing for selection on synonymous sites in plant mitochondrial DNA: the role of codon bias and RNA editing
Yang et al. A genome-phenome association study in native microbiomes identifies a mechanism for cytosine modification in DNA and RNA
Wei et al. Engineering eukaryotic transposon-encoded Fanzor2 system for genome editing in mammals
Wang et al. Characterization and engineering of a novel miniature Eubacterium siraeum CRISPR-Cas12f system
WO2024017189A1 (en) Tnpb-based genome editor
Larsen et al. Computationally optimised DNA assembly of synthetic genes
Wu et al. Two compact Cas9 ortholog-based cytosine base editors expand the DNA targeting scope and applications in vitro and in vivo
WO2024033411A1 (en) Methods for determining the location of a target sequence and uses
WO2024230784A1 (en) Methods and systems for identification of sequence adjacent motif, potency, and fidelity of a nuclease
RU2794774C1 (ru) Система редактирования генома crispr/cas9 ii типа и ее применение
Varapula et al. Recent Applications of CRISPR-Cas9 in Genome Mapping and Sequencing
Li et al. The intragenomic polymorphism of a partially inverted repeat (PIR) in Gallus gallus domesticus, potential role of inverted repeats in satellite DNAs evolution
Shen et al. Discovery and Engineering of a New BvCas12a Nuclease for Mammalian Genome Editing and Nucleic Acid Detection

Legal Events

Date Code Title Description
MK4 Application lapsed section 142(2)(d) - no continuation fee paid for the application