IL166285A0 - Methods for perfusion and plating of primary hepatocytes and a medium therefore - Google Patents

Methods for perfusion and plating of primary hepatocytes and a medium therefore

Info

Publication number
IL166285A0
IL166285A0 IL16628505A IL16628505A IL166285A0 IL 166285 A0 IL166285 A0 IL 166285A0 IL 16628505 A IL16628505 A IL 16628505A IL 16628505 A IL16628505 A IL 16628505A IL 166285 A0 IL166285 A0 IL 166285A0
Authority
IL
Israel
Prior art keywords
perfusion
plating
medium
methods
primary hepatocytes
Prior art date
Application number
IL16628505A
Other languages
English (en)
Original Assignee
Massachusetts Inst Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Inst Technology filed Critical Massachusetts Inst Technology
Publication of IL166285A0 publication Critical patent/IL166285A0/xx

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
IL16628505A 2002-07-30 2005-01-13 Methods for perfusion and plating of primary hepatocytes and a medium therefore IL166285A0 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39949302P 2002-07-30 2002-07-30
PCT/US2003/023869 WO2004011622A2 (en) 2002-07-30 2003-07-30 Methods for perfusion and plating of primary hepatocytes and a medium therefore

Publications (1)

Publication Number Publication Date
IL166285A0 true IL166285A0 (en) 2006-01-15

Family

ID=31188589

Family Applications (1)

Application Number Title Priority Date Filing Date
IL16628505A IL166285A0 (en) 2002-07-30 2005-01-13 Methods for perfusion and plating of primary hepatocytes and a medium therefore

Country Status (9)

Country Link
US (2) US20060110826A1 (de)
EP (1) EP1534821A4 (de)
JP (1) JP2005534303A (de)
KR (1) KR20050035870A (de)
CN (1) CN1685038A (de)
AU (1) AU2003261303B2 (de)
CA (1) CA2493676A1 (de)
IL (1) IL166285A0 (de)
WO (1) WO2004011622A2 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2921257B1 (fr) * 2007-09-21 2009-11-06 Exsymol Sa Utilisation topique de derives thiazolidines contre les consequences du stress oxydatif de la peau
KR20110074773A (ko) 2008-10-24 2011-07-01 가부시키가이샤 구라레 세포 배양 키트, 스크리닝 방법 및 세포 배양 키트의 제조 방법
CN102061284A (zh) * 2010-06-13 2011-05-18 南方医科大学珠江医院 人原代肝细胞分离培养方法
CN102220278A (zh) * 2011-04-29 2011-10-19 南方医科大学 一种分离原代成人肝细胞的方法及其专用无菌器械盒
WO2017202855A1 (en) * 2016-05-26 2017-11-30 F. Hoffmann-La Roche Ag Assay for evaluating anti-hbv effect
CN108070551A (zh) * 2016-11-15 2018-05-25 江苏齐氏生物科技有限公司 一种人原代肝细胞的分离及培养方法
CN109402044A (zh) * 2018-11-12 2019-03-01 苏州瑞徕生物科技有限公司 一种共培养肝细胞和肝窦内皮细胞的技术方法及其应用
CN115279893A (zh) * 2020-03-16 2022-11-01 味之素株式会社 动物细胞的培养方法
CN114586768A (zh) * 2020-12-03 2022-06-07 江苏齐氏生物科技有限公司 一种肝组织保存液及其保存方法和应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62285871A (ja) * 1986-06-03 1987-12-11 Canon Inc シ−ト斜行補正装置
US5198432A (en) * 1988-01-29 1993-03-30 Center For Innovative Technology Method of preventing chlorohydrocarbon toxicity using sterol derivatives
US6231881B1 (en) * 1992-02-24 2001-05-15 Anton-Lewis Usala Medium and matrix for long-term proliferation of cells
JPH11225752A (ja) * 1998-02-16 1999-08-24 Jms Co Ltd 人工肝臓用肝細胞クローンおよび体外循環肝補助装置
JP3435458B2 (ja) * 2000-06-07 2003-08-11 独立行政法人産業技術総合研究所 初代肝細胞の単層シート構造体とその形成方法

Also Published As

Publication number Publication date
JP2005534303A (ja) 2005-11-17
US20090137034A1 (en) 2009-05-28
US20060110826A1 (en) 2006-05-25
KR20050035870A (ko) 2005-04-19
AU2003261303A1 (en) 2004-02-16
EP1534821A4 (de) 2006-10-11
CA2493676A1 (en) 2004-02-05
AU2003261303B2 (en) 2008-07-10
WO2004011622A2 (en) 2004-02-05
EP1534821A2 (de) 2005-06-01
WO2004011622A3 (en) 2004-08-26
CN1685038A (zh) 2005-10-19

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Legal Events

Date Code Title Description
FF Patent granted
MM9K Patent not in force due to non-payment of renewal fees