IL104031A - Gel electrophoresis device and process - Google Patents
Gel electrophoresis device and processInfo
- Publication number
- IL104031A IL104031A IL10403192A IL10403192A IL104031A IL 104031 A IL104031 A IL 104031A IL 10403192 A IL10403192 A IL 10403192A IL 10403192 A IL10403192 A IL 10403192A IL 104031 A IL104031 A IL 104031A
- Authority
- IL
- Israel
- Prior art keywords
- membrane
- gel
- electrophoresis
- module
- electrophoretic transfer
- Prior art date
Links
- 238000001502 gel electrophoresis Methods 0.000 title claims abstract description 9
- 238000000034 method Methods 0.000 title claims description 22
- 230000008569 process Effects 0.000 title claims description 9
- 239000012528 membrane Substances 0.000 claims abstract description 153
- 238000012546 transfer Methods 0.000 claims abstract description 60
- 239000012634 fragment Substances 0.000 claims abstract description 24
- 238000012545 processing Methods 0.000 claims abstract description 19
- 238000000926 separation method Methods 0.000 claims abstract description 19
- 238000001514 detection method Methods 0.000 claims abstract description 8
- 238000001962 electrophoresis Methods 0.000 claims description 40
- 239000000872 buffer Substances 0.000 claims description 18
- 239000000243 solution Substances 0.000 claims description 11
- 239000007853 buffer solution Substances 0.000 claims description 10
- 238000005266 casting Methods 0.000 claims description 4
- 230000003247 decreasing effect Effects 0.000 claims 1
- 239000012530 fluid Substances 0.000 claims 1
- 239000000499 gel Substances 0.000 description 79
- 239000000463 material Substances 0.000 description 11
- 239000007788 liquid Substances 0.000 description 8
- 150000002500 ions Chemical class 0.000 description 6
- 230000008901 benefit Effects 0.000 description 5
- 230000007246 mechanism Effects 0.000 description 5
- 241000894007 species Species 0.000 description 5
- 230000032258 transport Effects 0.000 description 5
- 239000000523 sample Substances 0.000 description 4
- 239000004677 Nylon Substances 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 230000005684 electric field Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229920001778 nylon Polymers 0.000 description 3
- 238000006424 Flood reaction Methods 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 239000003792 electrolyte Substances 0.000 description 2
- 239000008273 gelatin Substances 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
- 235000019322 gelatine Nutrition 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 230000002285 radioactive effect Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 230000007723 transport mechanism Effects 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 241000206672 Gelidium Species 0.000 description 1
- 101100049053 Mus musculus Vash1 gene Proteins 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 235000010419 agar Nutrition 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229920006351 engineering plastic Polymers 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000002218 isotachophoresis Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000000615 nonconductor Substances 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 230000037390 scarring Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000005549 size reduction Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000007693 zone electrophoresis Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Electrotherapy Devices (AREA)
- Electrostatic Separation (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US81651391A | 1991-12-31 | 1991-12-31 | |
| US07/945,553 US5234559A (en) | 1991-12-31 | 1992-09-16 | Apparatus for direct blotting and automated electrophoresis, transfer and detection and processes utilizing the apparatus thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL104031A0 IL104031A0 (en) | 1993-05-13 |
| IL104031A true IL104031A (en) | 1995-03-30 |
Family
ID=27124085
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL10403192A IL104031A (en) | 1991-12-31 | 1992-12-09 | Gel electrophoresis device and process |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US5234559A (de) |
| EP (1) | EP0619882B1 (de) |
| JP (1) | JP3190342B2 (de) |
| AT (1) | ATE199780T1 (de) |
| AU (1) | AU3150393A (de) |
| CA (1) | CA2125949A1 (de) |
| DE (1) | DE69231738T2 (de) |
| IL (1) | IL104031A (de) |
| MX (1) | MX9207629A (de) |
| WO (1) | WO1993013412A1 (de) |
Families Citing this family (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5440822A (en) * | 1993-07-16 | 1995-08-15 | Novel Experimental Technology | Method and apparatus for drying electrophoresis gels |
| US5433837A (en) * | 1993-08-27 | 1995-07-18 | E. I. Du Pont De Nemours And Company | Gel cassette for enhanced electrophoretic separation and processes for the preparation thereof |
| US5474663A (en) * | 1993-08-27 | 1995-12-12 | E. I. Du Pont De Nemours And Company | Bowed framed membrane, processes for the preparation thereof, and uses therefor |
| US5449446A (en) * | 1994-03-09 | 1995-09-12 | Verma; Sumeet | Multi-purpose electrophoresis apparatus |
| US5800993A (en) * | 1994-07-29 | 1998-09-01 | The Regents Of The University Of California | DNA sequencing apparatus and method for a small format gel with a magnified readout |
| US5514256A (en) * | 1994-10-28 | 1996-05-07 | Battelle Memorial Institute | Apparatus for improved DNA sequencing |
| JP3976356B2 (ja) * | 1995-03-31 | 2007-09-19 | シスメックス株式会社 | 自動標本作製装置 |
| US6017496A (en) | 1995-06-07 | 2000-01-25 | Irori | Matrices with memories and uses thereof |
| US5916429A (en) * | 1997-08-01 | 1999-06-29 | Qualicon Inc. | Direct blot electrophoresis apparatus and method |
| US5939022A (en) * | 1997-12-09 | 1999-08-17 | Pharmacia Biotech, Inc. | Article for transporting biological samples during analysis |
| AU3102801A (en) * | 2000-01-19 | 2001-07-31 | Exact Sciences Corporation | Methods of and apparatus for separating and detecting nucleic acid |
| US7435322B2 (en) * | 2001-02-01 | 2008-10-14 | Hartmut Schlichting | Device and method for automatically analysing the constituents of an analyte |
| US20050233390A1 (en) * | 2003-04-09 | 2005-10-20 | Allen John W | Device including a proteinaceous factor, a recombinant proteinaceous factor, and a nucleotide sequence encoding the proteinaceous factor |
| WO2007081001A1 (ja) * | 2006-01-13 | 2007-07-19 | Arkray, Inc. | 遮光手段を備えた分析装置 |
| JP4813244B2 (ja) * | 2006-04-25 | 2011-11-09 | シャープ株式会社 | サンプル分離吸着器具 |
| US20080296158A1 (en) * | 2007-05-31 | 2008-12-04 | Sharp Kabushiki Kaisha | Device for electrophoresis, device for transfer, device for electrophoresis and transfer, chip for electrophoresis and transfer, and method for electrophoresis, method for transfer, and method for electrophoresis and transfer |
| US8399083B2 (en) * | 2007-08-06 | 2013-03-19 | Randy G. Cowan | Label structure incorporating a leaflet for use on small containers |
| JP4431838B2 (ja) * | 2008-06-30 | 2010-03-17 | シャープ株式会社 | サンプル分離吸着器具 |
| JP5702096B2 (ja) * | 2010-09-15 | 2015-04-15 | シャープ株式会社 | 電気泳動用カセット、そのパッケージ、および電気泳動方法 |
| JP5748121B2 (ja) * | 2011-03-04 | 2015-07-15 | シャープ株式会社 | 分離吸着キット、分離吸着装置及び分子の解析方法 |
| US9046454B2 (en) | 2011-06-08 | 2015-06-02 | Bio-Rad Laboratories, Inc. | Processing of analyte supports with oscillating fluid by interrupted rotation |
| BR112014028761A2 (pt) * | 2012-05-31 | 2017-06-27 | Ge Healthcare Bio Sciences Ab | agregado de supressão de pó, e, método de formação de um agregado de supressão de pó |
| JPWO2015151768A1 (ja) * | 2014-03-31 | 2017-04-13 | シャープ株式会社 | サンプル分離吸着用分離媒体カセットおよびサンプル分離吸着用分析装置 |
| US10126264B2 (en) | 2014-07-14 | 2018-11-13 | Li-Cor, Inc. | Analyte separator with electrohydrodynamic Taylor cone jet blotter |
| JP5952379B2 (ja) | 2014-11-20 | 2016-07-13 | シャープ株式会社 | 生体分子分析装置 |
| JP6067655B2 (ja) * | 2014-11-20 | 2017-01-25 | シャープ株式会社 | フレーム部材付き転写膜、生体分子分析装置、試薬槽及び振盪装置 |
| KR101855608B1 (ko) * | 2014-12-17 | 2018-05-04 | 샤프 라이프 사이언스 가부시키가이샤 | 생체 분자 분석 장치 |
| US10041906B2 (en) | 2015-03-03 | 2018-08-07 | Bio-Rad Laboratories, Inc. | Electrophoresis and electroblotting systems and methods |
| JP6030691B1 (ja) | 2015-04-10 | 2016-11-24 | シャープ株式会社 | サンプル分離転写装置およびサンプル分析方法 |
| AU2017213725B2 (en) | 2016-02-01 | 2021-12-23 | Li-Cor, Inc. | Capillary electrophoresis inkjet dispensing |
| US10921285B2 (en) * | 2016-03-04 | 2021-02-16 | Merck Ltd. | Sample separating implement and sample analyzing device |
| JP6461046B2 (ja) * | 2016-06-07 | 2019-01-30 | シャープライフサイエンス株式会社 | 転写膜保持器具及び分離転写装置 |
| US11241689B2 (en) | 2016-08-08 | 2022-02-08 | Li-Cor, Inc. | Microchip electrophoresis inkjet dispensing |
| CN109564187A (zh) | 2016-08-08 | 2019-04-02 | 利康公司 | 用于微流体直接印迹的多鞘流和芯片上的终端电极 |
| JP7130200B2 (ja) * | 2017-10-25 | 2022-09-05 | 日本光電工業株式会社 | 心筋細胞を含むシート状組織の張力測定デバイス、システム及びキット |
| KR102136719B1 (ko) * | 2018-02-13 | 2020-07-22 | 울산대학교 산학협력단 | 유기물 분석장치 |
| US12449396B2 (en) * | 2020-07-28 | 2025-10-21 | Life Technologies Corporation | Electrotransfer and electrophoresis devices, systems, and methods |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3062731A (en) * | 1959-11-18 | 1962-11-06 | Beckman Instruments Inc | Agar-agar system and additive |
| US3129158A (en) * | 1961-01-24 | 1964-04-14 | Raymond Samuel | Process for gel electrophoresis |
| US3047489A (en) * | 1961-05-25 | 1962-07-31 | Raymond Samuel | Apparatus for zone electrophoresis |
| GB1333796A (en) * | 1970-06-02 | 1973-10-17 | Lkb Produkter Ab | Electrophoresis |
| US3674678A (en) * | 1970-10-28 | 1972-07-04 | Millipore Corp | Electrophoretic apparatus |
| SE386742B (sv) * | 1973-11-14 | 1976-08-16 | Lkb Produkter Ab | Apparat for preparativ, isotachoforetisk separation i mikroskala |
| US4152390A (en) * | 1976-12-17 | 1979-05-01 | Eastman Kodak Company | Chemical analyzer |
| USRE30595E (en) * | 1978-06-05 | 1981-04-28 | Eastman Kodak Company | Article container for dispensing reagent slides |
| DE3022527C2 (de) * | 1980-06-16 | 1985-01-31 | Fritz Prof. Dr. 7750 Konstanz Pohl | Verfahren zur elektrophoretischen Trennung sowie Vorrichtung dazu |
| GB2147609B (en) * | 1983-10-10 | 1987-12-02 | Health Lab Service Board | Electrophoretic transfer |
| US4631422A (en) * | 1983-12-19 | 1986-12-23 | Rca Corporation | TTL circuit with a clamping transistor for speedy turn-off of output transistor |
| US4589965A (en) * | 1984-11-14 | 1986-05-20 | International Biotechnologies, Inc. | Method for electroblotting |
| US4622124A (en) * | 1985-01-11 | 1986-11-11 | International Biotechnologies, Inc. | Device for horizontal electroblotting |
| GB8523801D0 (en) * | 1985-09-26 | 1985-10-30 | Jones K W | Vacuum molecular transfer(blotting)apparatus |
| US4726889A (en) * | 1986-02-04 | 1988-02-23 | Oncor, Inc. | Process and apparatus for conducting electrophoresis and transfer |
| US4818360A (en) * | 1987-08-28 | 1989-04-04 | Bios Corporation | Method and apparatus for blotting from electrophoresis gels |
| US4812216A (en) * | 1987-08-28 | 1989-03-14 | Bios Corporation | Method of handling and transporting a transfer membrane used in a blotting apparatus |
| US4913791A (en) * | 1987-08-28 | 1990-04-03 | Bios Corporation | Blot frame and method of handling |
| GB8724528D0 (en) * | 1987-10-20 | 1987-11-25 | Amersham Int Plc | Biological testing |
| JPH01112147A (ja) * | 1987-10-26 | 1989-04-28 | Shimadzu Corp | 核酸の塩基配列決定方法 |
| FR2639253B2 (fr) * | 1988-09-06 | 1991-07-26 | Bertin & Cie | Dispositif d'electrophorese multiple pour assurer la migration controlee de macromolecules dans des plaques rectangulaires de gel |
| US4994166A (en) * | 1989-08-22 | 1991-02-19 | Bio-Rad Laboratories, Inc. | Single apparatus for slab gel electrophoresis and blotting |
| US5013420A (en) * | 1990-04-19 | 1991-05-07 | Life Technologies Inc. | Gel electrophoresis/electro-blot apparatus |
| JPH04213056A (ja) * | 1990-12-06 | 1992-08-04 | Sumitomo Electric Ind Ltd | 物質分画法および分画装置 |
-
1992
- 1992-09-16 US US07/945,553 patent/US5234559A/en not_active Expired - Lifetime
- 1992-12-03 WO PCT/US1992/010281 patent/WO1993013412A1/en not_active Ceased
- 1992-12-03 CA CA002125949A patent/CA2125949A1/en not_active Abandoned
- 1992-12-03 JP JP51166093A patent/JP3190342B2/ja not_active Expired - Lifetime
- 1992-12-03 EP EP92925457A patent/EP0619882B1/de not_active Expired - Lifetime
- 1992-12-03 AU AU31503/93A patent/AU3150393A/en not_active Abandoned
- 1992-12-03 AT AT92925457T patent/ATE199780T1/de active
- 1992-12-03 DE DE69231738T patent/DE69231738T2/de not_active Expired - Lifetime
- 1992-12-09 IL IL10403192A patent/IL104031A/en not_active IP Right Cessation
- 1992-12-30 MX MX9207629A patent/MX9207629A/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO1993013412A1 (en) | 1993-07-08 |
| CA2125949A1 (en) | 1993-07-08 |
| IL104031A0 (en) | 1993-05-13 |
| ATE199780T1 (de) | 2001-03-15 |
| DE69231738T2 (de) | 2001-08-09 |
| AU3150393A (en) | 1993-07-28 |
| JPH07502598A (ja) | 1995-03-16 |
| EP0619882B1 (de) | 2001-03-14 |
| US5234559A (en) | 1993-08-10 |
| DE69231738D1 (de) | 2001-04-19 |
| EP0619882A1 (de) | 1994-10-19 |
| JP3190342B2 (ja) | 2001-07-23 |
| MX9207629A (es) | 1993-07-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RH | Patent void |