IE40600B1 - Process for the isolation of apolyvalent proteinase inhibitor - Google Patents
Process for the isolation of apolyvalent proteinase inhibitorInfo
- Publication number
- IE40600B1 IE40600B1 IE280/75A IE28075A IE40600B1 IE 40600 B1 IE40600 B1 IE 40600B1 IE 280/75 A IE280/75 A IE 280/75A IE 28075 A IE28075 A IE 28075A IE 40600 B1 IE40600 B1 IE 40600B1
- Authority
- IE
- Ireland
- Prior art keywords
- inhibitor
- acid resins
- proteinase inhibitor
- apolyvalent
- isolation
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 239000000137 peptide hydrolase inhibitor Substances 0.000 title abstract 2
- 238000002955 isolation Methods 0.000 title 1
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 abstract 3
- 229920005989 resin Polymers 0.000 abstract 3
- 239000011347 resin Substances 0.000 abstract 3
- ABLZXFCXXLZCGV-UHFFFAOYSA-N Phosphorous acid Chemical group OP(O)=O ABLZXFCXXLZCGV-UHFFFAOYSA-N 0.000 abstract 2
- 239000002253 acid Substances 0.000 abstract 2
- 239000003463 adsorbent Substances 0.000 abstract 2
- 239000003112 inhibitor Substances 0.000 abstract 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 abstract 1
- 239000004793 Polystyrene Substances 0.000 abstract 1
- 229920006322 acrylamide copolymer Polymers 0.000 abstract 1
- 150000001408 amides Chemical class 0.000 abstract 1
- 210000001557 animal structure Anatomy 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- JXLHNMVSKXFWAO-UHFFFAOYSA-N azane;7-fluoro-2,1,3-benzoxadiazole-4-sulfonic acid Chemical compound N.OS(=O)(=O)C1=CC=C(F)C2=NON=C12 JXLHNMVSKXFWAO-UHFFFAOYSA-N 0.000 abstract 1
- 238000004132 cross linking Methods 0.000 abstract 1
- 150000002500 ions Chemical class 0.000 abstract 1
- 210000004072 lung Anatomy 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 229920002223 polystyrene Polymers 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 239000012266 salt solution Substances 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 abstract 1
- 239000003381 stabilizer Substances 0.000 abstract 1
- 230000001225 therapeutic effect Effects 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
- C07K14/8114—Kunitz type inhibitors
- C07K14/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The production of a polyvalent proteinase inhibitor from animal organs, in particular from lung, is carried out by adsorbing the inhibitor from its aqueous solutions by adsorption on an ion exchanger having functional sulphonate or phosphonate groups at pH 0.5 to 10.5 and eluting it with water or a salt solution at pH 1 to 13. A suitable adsorbent, in particular, is an ethenesulphonate/ acrylamide copolymer gel, as is obtained by copolymerisation of ethenesulphonic acid and acrylamide in the weight ratio 20:1 to 2:1 and subsequent crosslinking with 1 to 15 % formaldehyde. Further suitable adsorbents are polyphenolomega -sulphonic acid resins and also polystyrenesulphonic acid resins and polystyrene phosphonic acid resins. The inhibitor obtained by this process is suitable for therapeutic use on account of its high degree of purity. It can additionally be employed, for example, as a stabiliser for standardised protein preparations.
[GB1493960A]
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2406971A DE2406971B2 (en) | 1974-02-14 | 1974-02-14 | Process for obtaining a polyvalent proteinase inhibitor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IE40600L IE40600L (en) | 1975-08-14 |
| IE40600B1 true IE40600B1 (en) | 1979-07-04 |
Family
ID=5907334
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IE280/75A IE40600B1 (en) | 1974-02-14 | 1975-02-13 | Process for the isolation of apolyvalent proteinase inhibitor |
Country Status (14)
| Country | Link |
|---|---|
| JP (1) | JPS50111211A (en) |
| AT (1) | AT338414B (en) |
| BE (1) | BE825570A (en) |
| CH (1) | CH610909A5 (en) |
| DE (1) | DE2406971B2 (en) |
| DK (1) | DK145468B (en) |
| FR (1) | FR2261286B1 (en) |
| GB (1) | GB1493960A (en) |
| IE (1) | IE40600B1 (en) |
| IL (1) | IL46587A (en) |
| IT (1) | IT1043948B (en) |
| LU (1) | LU71826A1 (en) |
| NL (1) | NL7501488A (en) |
| SE (1) | SE7501654L (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2527222A1 (en) * | 1982-05-19 | 1983-11-25 | Christine Fougnot | METHOD FOR SEPARATING AND PURIFYING PROTEASES AND ANTIPROTEASES OF BLOOD COAGULATION, AS WELL AS PROTEASE / ANTIPROTEASE COMPLEX |
-
1974
- 1974-02-14 DE DE2406971A patent/DE2406971B2/en not_active Ceased
-
1975
- 1975-02-07 IL IL46587A patent/IL46587A/en unknown
- 1975-02-07 NL NL7501488A patent/NL7501488A/en not_active Application Discontinuation
- 1975-02-12 LU LU71826A patent/LU71826A1/xx unknown
- 1975-02-12 IT IT20216/75A patent/IT1043948B/en active
- 1975-02-13 AT AT107175A patent/AT338414B/en not_active IP Right Cessation
- 1975-02-13 JP JP50017517A patent/JPS50111211A/ja active Pending
- 1975-02-13 CH CH174675A patent/CH610909A5/en not_active IP Right Cessation
- 1975-02-13 DK DK52175AA patent/DK145468B/en not_active Application Discontinuation
- 1975-02-13 IE IE280/75A patent/IE40600B1/en unknown
- 1975-02-14 FR FR7504757A patent/FR2261286B1/fr not_active Expired
- 1975-02-14 BE BE153395A patent/BE825570A/en unknown
- 1975-02-14 SE SE7501654A patent/SE7501654L/ not_active Application Discontinuation
- 1975-02-14 GB GB6296/75A patent/GB1493960A/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| IE40600L (en) | 1975-08-14 |
| GB1493960A (en) | 1977-12-07 |
| IT1043948B (en) | 1980-02-29 |
| CH610909A5 (en) | 1979-05-15 |
| FR2261286B1 (en) | 1979-04-06 |
| BE825570A (en) | 1975-08-14 |
| AT338414B (en) | 1977-08-25 |
| LU71826A1 (en) | 1976-12-31 |
| IL46587A (en) | 1977-06-30 |
| DE2406971A1 (en) | 1975-08-21 |
| IL46587A0 (en) | 1975-04-25 |
| SE7501654L (en) | 1975-08-15 |
| DK52175A (en) | 1975-10-06 |
| DK145468B (en) | 1982-11-22 |
| FR2261286A1 (en) | 1975-09-12 |
| NL7501488A (en) | 1975-08-18 |
| JPS50111211A (en) | 1975-09-01 |
| DE2406971B2 (en) | 1979-08-09 |
| ATA107175A (en) | 1976-12-15 |
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