JPS5519082A - Preparation of urokinase - Google Patents

Preparation of urokinase

Info

Publication number
JPS5519082A
JPS5519082A JP9288678A JP9288678A JPS5519082A JP S5519082 A JPS5519082 A JP S5519082A JP 9288678 A JP9288678 A JP 9288678A JP 9288678 A JP9288678 A JP 9288678A JP S5519082 A JPS5519082 A JP S5519082A
Authority
JP
Japan
Prior art keywords
aqueous solution
resin
urokinase
polystyrene
crude urokinase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP9288678A
Other languages
Japanese (ja)
Other versions
JPS6040834B2 (en
Inventor
Gorou Shitao
Kazuo Morimoto
Masakazu Tajima
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Tanabe Pharma Corp
Original Assignee
Green Cross Corp Japan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Green Cross Corp Japan filed Critical Green Cross Corp Japan
Priority to JP9288678A priority Critical patent/JPS6040834B2/en
Publication of JPS5519082A publication Critical patent/JPS5519082A/en
Publication of JPS6040834B2 publication Critical patent/JPS6040834B2/en
Expired legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: To make it possible to adsorb and remove colored constituents from crude urokinase effectively, by bringing the aqueous solution of crude urokinase (U) into contact with a strongly basic ion excahnge resin consisting of a polystyrene- divinylbenzene copolymer having quaternary ammonium groups.
CONSTITUTION: An aqueous solution of crude urokinase (U), preferably a solution containing U with a specific activity of 10W1,000 IU/mg, obtained by an adsorbent, e.g. silica gel, is brought into contact with a strongly basic anion exchange resin having quaternary ammonium gooups as exchange groups, comprising a polystyrene-divinylbenzene copolymer as a substrate equilibrated with a buffer solution at pH 7W9, preferably 7.5W 8.5, to remove colored constituents and to recover the remaining aqueous solution of U. The amount of the resin is 20W 100ml per 100cc of the aqueous solution containing 1000IU/ml of U.
COPYRIGHT: (C)1980,JPO&Japio
JP9288678A 1978-07-28 1978-07-28 Production method of urokinase Expired JPS6040834B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP9288678A JPS6040834B2 (en) 1978-07-28 1978-07-28 Production method of urokinase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP9288678A JPS6040834B2 (en) 1978-07-28 1978-07-28 Production method of urokinase

Publications (2)

Publication Number Publication Date
JPS5519082A true JPS5519082A (en) 1980-02-09
JPS6040834B2 JPS6040834B2 (en) 1985-09-12

Family

ID=14066930

Family Applications (1)

Application Number Title Priority Date Filing Date
JP9288678A Expired JPS6040834B2 (en) 1978-07-28 1978-07-28 Production method of urokinase

Country Status (1)

Country Link
JP (1) JPS6040834B2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61227782A (en) * 1985-03-30 1986-10-09 Green Cross Corp:The Purification of urokinase and urokinase precursor
US7641527B1 (en) 2007-11-30 2010-01-05 Brp Us Inc. Marine outboard engine exhaust system

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61227782A (en) * 1985-03-30 1986-10-09 Green Cross Corp:The Purification of urokinase and urokinase precursor
US7641527B1 (en) 2007-11-30 2010-01-05 Brp Us Inc. Marine outboard engine exhaust system

Also Published As

Publication number Publication date
JPS6040834B2 (en) 1985-09-12

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